SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CCNA_02006 CCNA_02006 lipoprotein releasing system ATP-binding protein lolD to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
48% identity, 98% coverage: 1:223/228 of query aligns to 1:223/233 of P75957

query
sites
P75957

M

M

S

N

D

K

P

I

V

L

L

L

A

Q

L

C

R

D

G

N

L

L

E

C

R

K

V

R

Y

Y

K

Q

T

E

E

G

A

S

G

V

D

Q

L

T

P

D

V

V

L

L

R

H

G

N

V

V

D

S

L

F

D

S

V

V

Y

G

P

E

G

G

E

E

V

M

G

A

L

I

I

V

G
|
G

P

S

S

S

G

G

S

S

G

G

K

K

S

S

S

T

L

L

H

H

S

L

A

L

G

G

L

G

L

L

E

D

R

T

P

P

D

T

A

S

G

G

L

D

V

V

A

I

L

F

E

N

G

G

R

Q

D

P

C

M

S

S

K

K

L

L

S

S

E

S

R

A

A

A

R

K

T

A

R

E

I

L

R

R

L

N

G

Q

T

K

V

L

G

G

F

F

V

I

Y

Y

Q

Q

F

F

H

H

L

L

P

P

E

D

F

F

S

T

A

A

L

L

E

E

N

N

V

V

A

A

M

M

P

P

L

L

T

L

I

I

A

G

G

K

K

K

S

K

R

P

R

A

E

E

A

I

E

N

A

S

R

R

A

A

R

L

E

E

L

M

L

L

E

K

S

A

L

V

G

G

L

L

G

D

H

H

R

R

L

A

N

N

H

H

Q

R

P

P

A

S

Q

E

M

L

S

S

G

G

E

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

V

N

N

R

N

P

P

K

R

L

L

L

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

P

A

A

R

T

N

S

A

T

D

A

S

V

I

F

F

Q

Q

A

L

L

L

Y

G

Q

E

V

L

C

N

R

R

E

L

Q

Q

G

G

V

T

A

A

A

F

V

L

I

V

A

V

T

T

H

H

N

D

M

L

E

Q

L

L

A

A

R

K

Y

R

M

M

D

S

R

R

V

Q

V

L

A

E

L

M

K

R

D

D

G

G

H

R

L

L

G42 (= G42) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
49% identity, 96% coverage: 5:223/228 of query aligns to 2:220/226 of 7mdyC

query
sites
7mdyC

V

L

L

L

A

Q

L

C

R

D

G

N

L

L

E

C

R

K

V

R

Y
|
Y

K

Q

T

E

E

G

A

S

G

V

D

Q

L

T

P

D

V

V

L

L

R

H

G

N

V

V

D

S

L

F

D

S

V

V

Y

G

P

E

G

G

E

E

V

M

G

A

L

I

I

V

G

G

P

S

S

S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

S
x
T

L

L

H

H

S

L

A

L

G

G

L

G

L

L

E

D

R

T

P

P

D

T

A

S

G

G

L

D

V

V

A

I

L

F

E

N

G

G

R

Q

D

P

C

M

S

S

K

K

L

L

S

S

E

S

R

A

A

A

R

K

T

A

R

E

I

L

R

R

L

N

G

Q

T

K

V

L

G

G

F

F

V

I

Y

Y

Q
|
Q

F

F

H

H

L

L

P

P

E

D

F

F

S

T

A

A

L

L

E

E

N

N

V

V

A

A

M

M

P

P

L

L

T

L

I

I

A

G

G

K

K

K

S

K

R

P

R

A

E

E

A

I

E

N

A

S

R

R

A

A

R

L

E

E

L

M

L

L

E

K

S

A

L

V

G

G

L

L

G

D

H

H

R

R

L

A

N

N

H
|
H

Q

R

P

P

A

S

Q
x
E

M

L

S
|
S

G
|
G

E

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

V

N

N

R

N

P

P

K

R

L

L

L

V

L

L

A

A

D

D

E
|
E

P

P

T

T

G

G

N
|
N

L

L

D

D

P

A

A

R

T

N

S

A

T

D

A

S

V

I

F

F

Q

Q

A

L

L

L

Y

G

Q

E

V

L

C

N

R

R

E

L

Q

Q

G

G

V

T

A

A

A

F

V

L

I

V

A

V

T

T

H
|
H

N

D

M

L

E

Q

L

L

A

A

R

K

Y

R

M

M

D

S

R

R

V

Q

V

L

A

E

L

M

K

R

D

D

G

G

H

R

L

L

binding adp orthovanadate: Y12 (= Y15), G42 (= G45), S43 (= S46), G44 (= G47), K45 (= K48), S46 (= S49), T47 (≠ S50), Q91 (= Q94), H138 (= H141), E142 (≠ Q145), S144 (= S147), G145 (= G148), G146 (= G149), E168 (= E171), N172 (= N175), H201 (= H204)
binding magnesium ion: S46 (= S49), Q91 (= Q94)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
49% identity, 96% coverage: 5:223/228 of query aligns to 2:220/222 of 7arlD

query
sites
7arlD

V

L

L

L

A

Q

L

C

R

D

G

N

L

L

E

C

R

K

V

R

Y

Y

K

Q

T

E

E

G

A

S

G

V

D

Q

L

T

P

D

V

V

L

L

R

H

G

N

V

V

D

S

L

F

D

S

V

V

Y

G

P

E

G

G

E

E

V

M

G

A

L

I

I

V

G

G

P

S

S

S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

S

T

L

L

H

H

S

L

A

L

G

G

L

G

L

L

E

D

R

T

P

P

D

T

A

S

G

G

L

D

V

V

A

I

L

F

E

N

G

G

R

Q

D

P

C

M

S

S

K

K

L

L

S

S

E

S

R

A

A

A

R

K

T

A

R

E

I

L

R

R

L

N

G

Q

T

K

V

L

G

G

F

F

V

I

Y

Y

Q

Q

F

F

H

H

L

L

P

P

E

D

F

F

S

T

A

A

L

L

E

E

N

N

V

V

A

A

M

M

P

P

L

L

T

L

I

I

A

G

G

K

K

K

S

K

R

P

R

A

E

E

A

I

E

N

A

S

R

R

A

A

R

L

E

E

L

M

L

L

E

K

S

A

L

V

G

G

L

L

G

D

H

H

R

R

L

A

N

N

H

H

Q

R

P

P

A

S

Q

E

M

L

S

S

G

G

E

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

V

N

N

R

N

P

P

K

R

L

L

L

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

P

A

A

R

T

N

S

A

T

D

A

S

V

I

F

F

Q

Q

A

L

L

L

Y

G

Q

E

V

L

C

N

R

R

E

L

Q

Q

G

G

V

T

A

A

A

F

V

L

I

V

A

V

T

T

H

H

N

D

M

L

E

Q

L

L

A

A

R

K

Y

R

M

M

D

S

R

R

V

Q

V

L

A

E

L

M

K

R

D

D

G

G

H

R

L

L

binding adenosine-5'-diphosphate: G42 (= G45), G44 (= G47), K45 (= K48), S46 (= S49)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
48% identity, 96% coverage: 5:223/228 of query aligns to 4:222/229 of 7v8iD

query
sites
7v8iD

V

L

L

L

A

Q

L

C

R

D

G

N

L

L

E

C

R

K

V

R

Y

Y

K

Q

T

E

E

G

A

S

G

V

D

Q

L

T

P

D

V
|
V

L

L

R

H

G

N

V

V

D

S

L

F

D

S

V

V

Y

G

P

E

G

G

E

E

V

M

G

A

L

I

I

V

G

G

P

S

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

S
x
T

L

L

H

H

S

L

A

L

G

G

L

G

L

L

E

D

R

T

P

P

D

T

A

S

G

G

L

D

V

V

A

I

L

F

E

N

G

G

R

Q

D

P

C

M

S

S

K

K

L

L

S

S

E

S

R

A

A

A

R

K

T

A

R

E

I

L

R

R

L

N

G

Q

T

K

V

L

G

G

F

F

V

I

Y

Y

Q
|
Q

F

F

H

H

L

L

P

P

E

D

F

F

S

T

A

A

L

L

E

E

N

N

V

V

A

A

M

M

P

P

L

L

T

L

I

I

A

G

G

K

K

K

S

K

R

P

R

A

E

E

A

I

E

N

A

S

R

R

A

A

R

L

E

E

L

M

L

L

E

K

S

A

L

V

G

G

L

L

G

D

H

H

R
|
R

L

A

N

N

H
|
H

Q

R

P

P

A

S

Q
x
E

M

L

S
|
S

G

G

G
|
G

E
|
E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

V

N

N

R

N

P

P

K

R

L

L

L

V

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

N

L

L

D

D

P

A

A

R

T

N

S

A

T

D

A

S

V

I

F

F

Q

Q

A

L

L

L

Y

G

Q

E

V

L

C

N

R

R

E

L

Q

Q

G

G

V

T

A

A

A

F

V

L

I

V

A

V

T

T

H
|
H

N

D

M

L

E

Q

L

L

A

A

R

K

Y

R

M

M

D

S

R

R

V

Q

V

L

A

E

L

M

K

R

D

D

G

G

H

R

L

L

binding phosphoaminophosphonic acid-adenylate ester: V23 (= V24), S43 (= S44), G44 (= G45), G46 (= G47), K47 (= K48), S48 (= S49), T49 (≠ S50), Q93 (= Q94), R137 (= R138), H140 (= H141), E144 (≠ Q145), S146 (= S147), G148 (= G149), E149 (= E150), H203 (= H204)
binding magnesium ion: S48 (= S49), Q93 (= Q94)

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
49% identity, 90% coverage: 24:228/228 of query aligns to 18:223/223 of 2pclA

query
sites
2pclA

V

I

L

L

R

K

G

G

V

I

D

S

L

L

D

S

V

V

Y

K

P

K

G

G

E

E

V

F

V

V

G

S

L

I

I

I

G

G

P

A

S

S

G

G

S

S

G

G

K

K

S

S

S

T

L

L

H

Y

S

I

A

L

G

G

L

L

E

D

R

A

P

P

D

T

A

E

G

G

L

K

V

V

A

F

L

L

E

E

G

G

R

K

D

E

C

V

S

D

K

Y

L

T

S

N

E

E

R

K

A

E

R

L

T

S

R

L

I

L

R

R

L

N

G

R

T

K

V

L

G

G

F

F

V

V

Y

F

Q

Q

F

F

H

H

H

Y

L

L

L

I

P

P

E

E

F

L

S

T

A

A

L

L

E

E

N

N

V

V

A

I

M

V

P

P

L

M

T

L

I

K

A

M

G

G

K

K

S

P

R

K

E

E

A

A

E

K

A

E

R

R

A

G

R

E

E

Y

L

L

E

S

S

E

L

L

G

G

L

L

G

G

H

D

R

K

L

L

N

S

H

R

Q

K

P

P

A

Y

Q

E

M

L

S

S

G

G

E

E

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

N

N

R

E

P

P

K

I

L

L

L

F

A

A

D

D

E
|
E

P
|
P

T
|
T

G

G

N

N

L

L

D

D

P

S

A

A

T

N

S

T

T

K

A

R

V

V

F

M

Q

D

A

I

L

F

Y

L

Q

K

V

I

C

-

R

N

E

E

Q

G

G

G

V

T

A

S

A

I

V

V

I

M

A

V

T
|
T

H

H

N

E

M

R

E

E

L

L

A

A

R

E

Y

L

M

T

D

H

R

R

V

T

V

L

A

E

L

M

K

K

D

D

G

G

H

K

L

V

-

V

-

G

E

E

L

I

Q

T

R

R

V

V

binding magnesium ion: E165 (= E171), P166 (= P172), T167 (= T173), T196 (= T203)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
41% identity, 96% coverage: 5:224/228 of query aligns to 1:224/232 of 1f3oA

query
sites
1f3oA

V

M

L

I

A

K

L

L

R

K

G

N

L

V

E

T

R

K

V

T

Y
|
Y

K

K

T

M

E

G

A

E

G

E

D

I

L

I

P

Y

V

A

L

L

R

K

G

N

V

V

D

N

L

L

D

N

V

I

Y

K

P

E

G

G

E

E

V

F

V

V

G

S

L

I

I

M

G

G

P

P

S

S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

S
x
T

L

M

L

L

H

N

S

I

A

I

G

G

L

C

L

L

E

D

R

K

P

P

D

T

A

E

G

G

L

E

V

V

A

Y

L

I

E

D

G

N

R

I

D

K

C

T

S

N

K

D

L

L

S

D

E

D

R

D

A

E

R

L

T

T

R

K

I

I

R

R

L

R

G

D

T

K

V

I

G

G

F

F

V

V

Y

F

Q

Q

F

Q

H

F

H

N

L

L

L

I

P

P

E

L

F

L

S

T

A

A

L

L

E

E

N

N

V

V

A

E

M

L

P

P

L

L

T

I

-

F

-

K

-

Y

-

R

-

G

-

A

I

M

A

S

G

G

K

E

S

E

R

R

E

K

A

-

E

-

A

-

R

R

A

A

R

L

E

E

L

C

L

L

E

K

S

M

L

A

G

E

L

L

G

E

H

E

R

R

L

F

-

A

N

N

H

H

Q

K

P

P

A

N

Q

Q

M

L

S

S

G

G

E

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

N

N

R

N

P

P

K

P

L

I

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

P

S

A

K

T

T

S

G

T

E

A

K

V

I

F

M

Q

Q

A

L

L

L

Y

K

Q

K

V

L

C

N

R

E

E

E

Q

D

G

G

V

K

A

T

A

V

V

V

I

V

A

V

T

T

H

H

N

D

M

I

E

N

L

V

A

A

R

R

Y

F

M

G

D

E

R

R

V

I

A

Y

L

L

K

K

D

D

G

G

H

E

L

V

E

E

binding adenosine-5'-diphosphate: Y11 (= Y15), G41 (= G45), G43 (= G47), K44 (= K48), S45 (= S49), T46 (≠ S50)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
41% identity, 96% coverage: 5:224/228 of query aligns to 1:224/230 of 1l2tA

query
sites
1l2tA

V

M

L

I

A

K

L

L

R

K

G

N

L

V

E

T

R

K

V

T

Y
|
Y

K

K

T

M

E

G

A

E

G

E

D

I

L

I

P

Y

V

A

L

L

R

K

G

N

V

V

D

N

L

L

D

N

V

I

Y

K

P

E

G

G

E

E

V

F

V

V

G

S

L

I

I

M

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

S
x
T

L

M

L

L

H

N

S

I

A

I

G

G

L

C

L

L

E

D

R

K

P

P

D

T

A

E

G

G

L

E

V

V

A

Y

L

I

E

D

G

N

R

I

D

K

C

T

S

N

K

D

L

L

S

D

E

D

R

D

A

E

R

L

T

T

R

K

I

I

R

R

L

R

G

D

T

K

V

I

G

G

F

F

V

V

Y

F

Q

Q

F

Q

H

F

H

N

L

L

L

I

P

P

E

L

F

L

S

T

A

A

L

L

E

E

N

N

V

V

A

E

M

L

P

P

L

L

T

I

-

F

-

K

-

Y

-

R

-

G

-

A

I

M

A

S

G

G

K

E

S

E

R

R

E

K

A

-

E

-

A

-

R

R

A

A

R

L

E

E

L

C

L

L

E

K

S

M

L

A

G

E

L

L

G

E

H

E

R

R

L
x
F

-

A

N

N

H

H

Q

K

P

P

A

N

Q
|
Q

M

L

S
|
S

G

G

G
|
G

E
x
Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

N

N

R

N

P

P

K

P

L

I

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

A

L

L

D

D

P

S

A

K

T

T

S

G

T

E

A

K

V

I

F

M

Q

Q

A

L

L

L

Y

K

Q

K

V

L

C

N

R

E

E

E

Q

D

G

G

V

K

A

T

A

V

V

V

I

V

A

V

T

T

H
|
H

N

D

M

I

E

N

L

V

A

A

R

R

Y

F

M

G

D

E

R

R

V

I

A

Y

L

L

K

K

D

D

G

G

H

E

L

V

E

E

binding adenosine-5'-triphosphate: Y11 (= Y15), S40 (= S44), G41 (= G45), S42 (= S46), G43 (= G47), K44 (= K48), S45 (= S49), T46 (≠ S50), F138 (≠ L139), Q145 (= Q145), S147 (= S147), G149 (= G149), Q150 (≠ E150), H204 (= H204)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
41% identity, 95% coverage: 5:221/228 of query aligns to 3:218/226 of 5xu1B

query
sites
5xu1B

V

L

L

I

A

S

L

L

R

K

G

N

L

I

E

F

R

R

V

S

Y

Y

K

R

T

N

E

G

A

D

G

Q

D

E

L

L

P

Q

V

V

L

L

R

K

G

N

V

I

D

N

L

L

D

E

V

V

Y

N

P

E

G

G

E

E

V

F

V

V

G

A

L

I

I

M

G

G

P

P

S

S

G

G

S

S

G
|
G

K
|
K

S
|
S

S

T

L

L

L

M

H

N

S

T

A

I

G

G

L

M

L

L

E

D

R

T

P

P

D

T

A

S

G

G

L

E

V

Y

A

Y

L

L

E

E

G

G

R

Q

D

E

C

V

S

A

K

G

L

L

S

G

E

E

R

K

A

Q

R

L

T

A

R

K

I

V

R

R

L

N

G

Q

T

Q

V

I

G

G

F

F

V

V

Y

F

Q

Q

F

Q

H

F

L

L

P

S

E

K

F

L

S

N

A

A

L

L

E

Q

N

N

V

V

A

E

M

L

P

P

L

L

T

I

I

Y

A

A

G

G

K

V

S

S

R

S

E

K

A

R

E

R

A

K

R

L

A

A

R

E

E

E

L

Y

L

L

E

D

S

K

L

V

G

E

L

L

G

T

H

E

R

R

L

S

N

H

H

H

Q

L

P

P

A

S

Q

E

M

L

S

S

G

G

E

Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

V

N

N

R

N

P

P

K

S

L

I

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

P

T

A

K

T

T

S

G

T

N

A

Q

V

I

F

M

Q

Q

A

L

L

L

Y

V

Q

D

V

L

C

N

R

K

E

E

Q

-

G

G

V

K

A

T

A

I

V

I

I

M

A

V

T

T

H

H

N

E

M

P

E

E

L

I

A

A

R

A

Y

Y

M

A

D

K

R

R

V

Q

V

I

A

V

L

I

K

R

D

D

G

G

binding magnesium ion: G45 (= G47), K46 (= K48), S47 (= S49)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
41% identity, 96% coverage: 4:223/228 of query aligns to 3:221/648 of P75831

query
sites
P75831

P

P

V

L

L

L

A

E

L

L

R

K

G

D

L

I

E

R

R

R

V

S

Y

Y

K

P

T

A

E

G

A

D

G

E

D

Q

L

V

P

E

V

V

L

L

R

K

G

G

V

I

D

S

L

L

D

D

V

I

Y

Y

P

A

G

G

E

E

V

M

V

V

G

A

L

I

I

V

G

G

P

A

S

S

G

G

S

S

G

G

K
|
K

S

S

S

T

L

L

L

M

H

N

S

I

A

L

G

G

L

C

L

L

E

D

R

K

P

A

D

T

A

S

G

G

L

T

V

Y

A

R

L

V

E

A

G

G

R

Q

D

D

C

V

S

A

K

T

L

L

S

D

E

A

R

D

A

A

R

L

T

A

R

Q

I

L

R

R

L

R

G

E

T

H

V

F

G

G

F

F

V

I

Y

F

Q

Q

F

R

H

Y

H

H

L

L

P

S

E

H

F

L

S

T

A

A

L

E

E

Q

N

N

V

V

A

E

M

V

P

P

L

A

T

V

I

Y

A

A

G

G

K

L

S

E

R

R

R

K

E

Q

A

R

E

L

A

L

R

R

A

A

R

Q

E

E

L

L

E

Q

S

R

L

L

G

G

L

L

G

E

H

D

R

R

L

T

N

E

H

Y

Q

Y

P

P

A

A

Q

Q

M

L

S

S

G

G

E

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

A

M

N

N

R

G

P

G

K

Q

L

V

L

I

L

L

A

A

D
|
D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

P

S

A

H

T

S

S

G

T

E

A

E

V

V

F

M

Q

A

A

I

L

L

Y

H

Q

Q

V

L

C

-

R

R

E

D

Q

R

G

G

V

H

A

T

A

V

V

I

I

I

A

V

T

T

H

H

N

D

M

P

E

Q

L

V

A

A

R

A

Y

Q

M

A

D

E

R

R

V

V

V

I

A

E

L

I

K

R

D

D

G

G

H

E

L

I

K47 (= K48) mutation to L: Lack of activity.
D169 (= D170) mutation to N: Lack of activity.

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
38% identity, 96% coverage: 5:223/228 of query aligns to 4:221/650 of 5ws4A

query
sites
5ws4A

V

L

L

L

A

E

L

V

R

S

G

N

L

L

E

V

R

R

V

E

Y

F

K

P

T

A

E

G

A

E

G

S

D

T

L

I

P

Q

V

I

L

L

R

K

G

G

V

I

D

D

L

L

D

T

V

I

Y

Y

P

E

G

G

E

E

V

L

V

V

G

A

L

I

I

V

G

G

P

Q

S
|
S

G
|
G

S

S

G

G

K

K

S
|
S

S

T

L

L

L

M

H

N

S

I

A

L

G

G

L

C

L

L

E

D

R

R

P

P

D

T

A

S

G

G

L

S

V

Y

A

K

L

V

E

N

G

G

R

Q

D

E

C

T

S

G

K

K

L

L

S

E

E

P

R

D

A

Q

R

L

T

A

R

Q

I

L

R

R

L

R

G

E

T

Y

V

F

G

G

F

F

V

I

Y

F

Q

Q

F

R

H

Y

H

H

L

L

P

G

E

D

F

L

S

S

A

A

L

E

E

G

N

N

V

V

A

E

M

V

P

P

L

A

T

V

I

Y

A

A

G

G

K

V

S

T

R

P

R

A

E

D

A

R

E

K

A

Q

R

R

A

A

R

T

E

A

L

L

E

T

S

E

L

L

G

G

L

L

G

G

H

T

R

K

L

T

N

Q

H

N

Q

R

P

P

A

S

Q

Q

M

L

S

S

G

G

E

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

A

M

N

N

R

G

P

G

K

D

L

V

L

I

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

P

S

A

H

T

S

S

G

T

V

A

E

V

V

F

M

Q

R

A

I

L

L

Y

R

Q

E

V

L

C

-

R

N

E

A

Q

A

G

G

V

H

A

T

A

I

V

I

I

L

A

V

T

T

H

H

N

D

M

M

E

Q

L

V

A

A

R

K

Y

N

M

A

D

T

R

R

V

I

A

E

L

I

K

S

D

D

G

G

H

E

L

I

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (= S44), G44 (= G45), S48 (= S49)

8w6iD Cryo-em structure of escherichia coli str k12 ftsex complex with atp- gamma-s in peptidisc
42% identity, 94% coverage: 11:224/228 of query aligns to 4:216/219 of 8w6iD

query
sites
8w6iD

L

F

E

E

R

H

V

V

Y

S

K

K

T

A

E
x
Y

A

L

G

G

D

G

L

R

P

Q

V

A

L

L

R

Q

G

G

V

V

D

T

L

F

D

H

V

M

Y

Q

P

P

G

G

E

E

V

M

V

A

G

F

L

L

I

T

G

G

P

H

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

S
x
T

L

L

H

K

S

L

A

I

G

C

L

G

L

I

E

E

R

R

P

P

D

S

A

A

G

G

L

K

V

I

A

W

L

F

E

S

G

G

R

H

D

D

C

I

S

T

K

R

L

L

S

K

E

N

R

R

A

E

R

V

T

P

R

F

I

L

R

R

L

R

G

-

T

Q

V

I

G

G

F

M

V

I

Y

F

Q
|
Q

F

D

H

H

L

L

P

M

E

D

F

R

S

T

A

V

L

Y

E

D

N

N

V

V

A

A

M

I

P

P

L

L

T

I

I

I

A

A

G

G

K

A

S

S

R

G

R

D

E

D

A

I

E

R

A

R

R

R

A

V

R

S

E

A

L

A

L

L

E

D

S

K

L

V

G

G

L

L

G

L

H

D

R
x
K

L

A

N

K

H

N

Q

F

P

P

A

I

Q
|
Q

M

L

S
|
S

G
|
G

E
|
E

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

L

V

A

V

N

N

R

K

P

P

K

A

L

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

P

D

A

A

T

L

S

S

T

E

A

G

V

I

F

L

Q

R

A

-

L

L

Y

F

Q

E

V

E

C

F

R

N

E

R

Q

V

G

G

V

V

A

T

A

V

V

L

I

M

A

A

T

T

H
|
H

N

D

M

I

E

N

L

L

-

I

A

S

R

R

Y

R

M

S

D

Y

R

R

V

M

V

L

A

T

L

L

K

S

D

D

G

G

H

H

L

L

E

H

binding phosphothiophosphoric acid-adenylate ester: Y11 (≠ E18), S37 (= S44), G38 (= G45), A39 (≠ S46), G40 (= G47), K41 (= K48), S42 (= S49), T43 (≠ S50), Q86 (= Q94), K130 (≠ R138), Q137 (= Q145), S139 (= S147), G140 (= G148), G141 (= G149), E142 (= E150), H195 (= H204)

P0A9R7 Cell division ATP-binding protein FtsE from Escherichia coli (strain K12) (see paper)
42% identity, 94% coverage: 11:224/228 of query aligns to 4:216/222 of P0A9R7

query
sites
P0A9R7

L

F

E

E

R

H

V

V

Y

S

K

K

T

A

E

Y

A

L

G

G

D

G

L

R

P

Q

V

A

L

L

R

Q

G

G

V

V

D

T

L

F

D

H

V

M

Y

Q

P

P

G

G

E

E

V

M

V

A

G

F

L

L

I

T

G

G

P

H

S

S

G

G

S

A

G

G

K
|
K

S

S

S

T

L

L

H

K

S

L

A

I

G
x
C

L

G

L

I

E

E

R

R

P

P

D

S

A

A

G

G

L

K

V

I

A

W

L

F

E

S

G

G

R

H

D

D

C

I

S

T

K

R

L

L

S

K

E

N

R

R

A

E

R

V

T

P

R

F

I

L

R

R

L

R

G

-

T

Q

V

I

G

G

F

M

V

I

Y

F

Q

Q

F

D

H

H

L

L

P

M

E

D

F

R

S

T

A

V

L

Y

E

D

N

N

V

V

A

A

M

I

P

P

L

L

T

I

I

I

A

A

G

G

K

A

S

S

R

G

R

D

E

D

A

I

E

R

A

R

R

R

A

V

R

S

E

A

L

A

L

L

E

D

S

K

L

V

G

G

L

L

G

L

H

D

R

K

L

A

N

K

H

N

Q

F

P

P

A

I

Q

Q

M

L

S

S

G

G

E

E

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

L

V

A

V

N

N

R

K

P

P

K

A

L

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

P

D

A

A

T

L

S

S

T

E

A

G

V

I

F

L

Q

R

A

-

L

L

Y

F

Q

E

V

E

C

F

R

N

E

R

Q

V

G

G

V

V

A

T

A

V

V

L

I

M

A

A

T

T

H

H

N

D

M

I

E

N

L

L

-

I

A

S

R

R

Y

R

M

S

D

Y

R

R

V

M

V

L

A

T

L

L

K

S

D

D

G

G

H

H

L

L

E

H

K41 (= K48) mutation to R: Does not bind ATP.
C49 (≠ G56) mutation to A: Prevents dimer formation. Does not alter ATP-binding.

8hd0A Cell divisome spg hydrolysis machinery ftsex-envc
42% identity, 94% coverage: 11:224/228 of query aligns to 4:216/218 of 8hd0A

query
sites
8hd0A

L

F

E

E

R

H

V

V

Y

S

K

K

T

A

E
x
Y

A

L

G

G

D

G

L
x
R

P

Q

V
x
A

L

L

R

Q

G

G

V

V

D

T

L

F

D

H

V

M

Y

Q

P

P

G

G

E

E

V

M

V

A

G

F

L

L

I

T

G

G

P

H

S

S

G

G

S

A

G
|
G

K
|
K

S
|
S

S
x
T

L

L

H

K

S

L

A

I

G

C

L

G

L

I

E

E

R

R

P

P

D

S

A

A

G

G

L

K

V

I

A

W

L

F

E

S

G

G

R

H

D

D

C

I

S

T

K

R

L

L

S

K

E

N

R

R

A

E

R

V

T

P

R

F

I

L

R

R

L

R

G

-

T

Q

V

I

G

G

F

M

V

I

Y

F

Q

Q

F

D

H

H

L

L

P

M

E

D

F

R

S

T

A

V

L

Y

E

D

N

N

V

V

A

A

M

I

P

P

L

L

T

I

I

I

A

A

G

G

K

A

S

S

R

G

R

D

E

D

A

I

E

R

A

R

R

R

A

V

R

S

E

A

L

A

L

L

E

D

S

K

L

V

G

G

L

L

G

L

H

D

R

K

L

A

N

K

H

N

Q

F

P

P

A

I

Q

Q

M

L

S
|
S

G

G

G
|
G

E

E

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

L

V

A

V

N

N

R

K

P

P

K

A

L

V

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

N

L

L

D

D

P

D

A

A

T

L

S

S

T

E

A

G

V

I

F

L

Q

R

A

-

L

L

Y

F

Q

E

V

E

C

F

R

N

E

R

Q

V

G

G

V

V

A

T

A

V

V

L

I

M

A

A

T

T

H

H

N

D

M

I

E

N

L

L

-

I

A

S

R

R

Y

R

M

S

D

Y

R

R

V

M

V

L

A

T

L

L

K

S

D

D

G

G

H

H

L

L

E

H

binding adenosine-5'-triphosphate: Y11 (≠ E18), R15 (≠ L22), A17 (≠ V24), G40 (= G47), K41 (= K48), S42 (= S49), T43 (≠ S50), S139 (= S147), G141 (= G149)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
36% identity, 96% coverage: 5:223/228 of query aligns to 3:220/592 of 5lj7A

query
sites
5lj7A

V

I

L

I

A

E

L

I

R

K

G

Q

L

L

E

N

R

R

V

Y

Y

F

K

G

T

E

E

G

A

E

G

N

D

R

L

V

P

H

V
|
V

L

L

R

K

G

D

V

I

D

S

L

L

D

S

V

I

Y

E

P

R

G

G

E

D

V

F

V

V

G

A

L

I

I

M

G

G

P

Q

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

S
x
T

L

L

L

M

H

N

S

I

A

I

G

G

L

C

L

L

E

D

R

T

P

A

D

T

A

G

G

G

L

S

V

S

A

K

L

I

E

D

G

G

R

K

D

E

C

T

S

I

K

E

L

L

S

T

E

N

R

D

A

Q

R

L

T

S

R

D

I

L

R

R

L

S

G

Q

T

K

V

F

G

G

F

F

V

I

Y

F

Q
|
Q

F

R

H

Y

H

N

L

L

P

S

E

S

F

L

S

T

A

A

L

A

E

E

N

N

V

V

A

A

M

L

P

P

L

A

T

I

I

Y

A

A

G

G

K

M

S

P

R

Q

R

S

E

Q

A

R

E

L

A

E

R

R

A

A

R

K

E

Q

L

L

E

E

S

K

L

L

G

G

L

L

G

G

H

D

R
x
K

L

W

N

Q

H

N

Q

K

P

P

A

N

Q
|
Q

M

L

S
|
S

G

G

G
|
G

E
x
Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

A

M

N

N

R

G

P

G

K

E

L

I

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

A

L

L

D

D

P

S

A

H

T

S

S

G

T

E

A

N

V

V

F

M

Q

E

A

I

L

L

Y

R

Q

Q

V

L

C

-

R

H

E

E

Q

E

G

G

V

H

A

T

A

I

V

I

I

M

A

V

T

T

H

H

N

D

M

K

E

H

L

I

A

A

R

A

Y

S

M

A

D

N

R

R

V

I

A

E

L

I

K

K

D

D

G

G

H

E

L

I

binding adenosine-5'-triphosphate: V22 (= V24), S42 (= S44), G43 (= G45), G45 (= G47), K46 (= K48), S47 (= S49), T48 (≠ S50), Q92 (= Q94), K136 (≠ R138), Q143 (= Q145), S145 (= S147), G147 (= G149), Q148 (≠ E150)
binding magnesium ion: S47 (= S49), Q92 (= Q94)

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
36% identity, 96% coverage: 5:223/228 of query aligns to 3:220/615 of 5lilA

query
sites
5lilA

V

I

L

I

A

E

L

I

R

K

G

Q

L

L

E

N

R

R

V

Y

Y
x
F

K

G

T

E

E

G

A

E

G

N

D

R

L

V

P

H

V
|
V

L

L

R

K

G

D

V

I

D

S

L

L

D

S

V

I

Y

E

P

R

G

G

E

D

V

F

V

V

G

A

L

I

I

M

G

G

P

Q

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

S
x
T

L

L

L

M

H

N

S

I

A

I

G

G

L

C

L

L

E

D

R

T

P

A

D

T

A

G

G

G

L

S

V

S

A

K

L

I

E

D

G

G

R

K

D

E

C

T

S

I

K

E

L

L

S

T

E

N

R

D

A

Q

R

L

T

S

R

D

I

L

R

R

L

S

G

Q

T

K

V

F

G

G

F

F

V

I

Y

F

Q
|
Q

F

R

H

Y

H

N

L

L

P

S

E

S

F

L

S

T

A

A

L

A

E

E

N

N

V

V

A

A

M

L

P

P

L

A

T

I

I

Y

A

A

G

G

K

M

S

P

R

Q

R

S

E

Q

A

R

E

L

A

E

R

R

A

A

R

K

E

Q

L

L

E

E

S

K

L

L

G

G

L

L

G

G

H

D

R
x
K

L

W

N

Q

H

N

Q

K

P

P

A

N

Q
|
Q

M

L

S
|
S

G

G

G
|
G

E
x
Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

A

M

N

N

R

G

P

G

K

E

L

I

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

A

L

L

D

D

P

S

A

H

T

S

S

G

T

E

A

N

V

V

F

M

Q

E

A

I

L

L

Y

R

Q

Q

V

L

C

-

R

H

E

E

Q

E

G

G

V

H

A

T

A

I

V

I

I

M

A

V

T

T

H

H

N

D

M

K

E

H

L

I

A

A

R

A

Y

S

M

A

D

N

R

R

V

I

A

E

L

I

K

K

D

D

G

G

H

E

L

I

binding adenosine-5'-triphosphate: F13 (≠ Y15), V22 (= V24), S42 (= S44), G43 (= G45), G45 (= G47), K46 (= K48), S47 (= S49), T48 (≠ S50), Q92 (= Q94), K136 (≠ R138), Q143 (= Q145), S145 (= S147), G147 (= G149), Q148 (≠ E150)
binding magnesium ion: S47 (= S49), Q92 (= Q94)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
42% identity, 91% coverage: 14:221/228 of query aligns to 7:212/240 of 4ymuJ

query
sites
4ymuJ

V

V

Y

Y

K

K

T

N

E

-

A
x
F

G

G

D

S

L

L

P

E

V
|
V

L

L

R

K

G

G

V

V

D

T

L

L

D

K

V

V

Y

N

P

K

G

G

E

E

V

V

G

V

L

I

I

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

S
x
T

L

L

H

R

S

C

A

I

G

N

L

L

E

E

R

E

P

P

D

T

A

K

G

G

L

E

V

V

A

F

L

I

E

D

G

G

R

V

D

K

C

I

S

N

K

N

L

-

S

G

E

K

R

V

A

N

R

I

T

N

R

K

I

V

R

R

L

-

G

Q

T

K

V

V

G

G

F

M

V

V

Y

F

Q

Q

F

H

H

F

H

N

L

L

L

F

P

P

E

H

F

L

S

T

A

A

L

I

E

E

N

N

V

I

A

T

M

L

-

A

P

P

L

V

T

K

I

V

A

K

G

K

K

M

S

N

R

K

E

E

A

A

E

E

A

E

R

L

A

A

R

V

E

D

L

L

E

A

S

K

L

V

G

G

L

L

G

L

H

D

R

K

L

K

N

D

H

Q

Q

Y

P

P

A

I

Q

K

M

L

S

S

G

G

E

Q

Q

K

Q

Q

R

R

V

L

A

A

I

I

A

A

R

R

A

A

L

L

A

A

N

M

R

Q

P

P

K

E

L

V

L

M

L

L

A

F

D

D

E
|
E

P

P

T

T

G

S

N

A

L

L

D

D

P

P

A

E

T

M

S

V

T

K

A

E

V

V

F

L

Q

N

A

V

L

M

Y

K

Q

Q

V

L

C

A

R

N

E

E

Q

-

G

G

V

M

A

T

A

M

V

V

I

V

A

V

T

T

H
|
H

N

E

M

M

E

G

L

F

A

A

R

R

Y

E

M

V

-

G

D

D

R

R

V

V

V

I

A

F

L

M

K

D

D

D

G

G

binding adenosine-5'-triphosphate: F11 (≠ A19), V16 (= V24), S36 (= S44), G37 (= G45), S38 (= S46), G39 (= G47), K40 (= K48), S41 (= S49), T42 (≠ S50), E162 (= E171), H194 (= H204)
binding magnesium ion: S41 (= S49), E162 (= E171)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
38% identity, 96% coverage: 5:223/228 of query aligns to 1:219/343 of P30750

query
sites
P30750

V

M

L

I

A

K

L

L

R

S

G

N

L

I

E

T

R

K

V

V

Y

F

K

H

T

Q

E

G

A

T

G

R

D

T

L

I

P

Q

V

A

L

L

R

N

G

N

V

V

D

S

L

L

D

H

V

V

Y

P

P

A

G

G

E

Q

V

I

V

Y

G

G

L

V

I

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

S
x
T

L

L

L

I

H

R

S

C

A

V

G

N

L

L

E

E

R

R

P

P

D

T

A

E

G

G

L

S

V

V

A

L

L

V

E

D

G

G

R

Q

D

E

C

L

S

T

K

T

L

L

S

S

E

E

R

S

A

E

R

L

T

T

R

K

I

A

R

R

L

-

G

R

T

Q

V

I

G

G

F

M

V

I

Y

F

Q

Q

F

H

H

F

H

N

L

L

P

S

E

S

F

R

S

T

A

V

L

F

E

G

N

N

V

V

A

A

M

L

P

P

L

L

T

E

I

L

A

D

G

N

K

T

S

P

R

K

R

D

E

E

A

V

E

K

A

R

R

R

A

V

R

T

E

E

L

L

E

S

S

L

L

V

G

G

L

L

G

G

H

D

R

K

L

H

N

D

H

S

Q

Y

P

P

A

S

Q

N

M

L

S

S

G

G

E

Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

N

S

R

N

P

P

K

K

L

V

L

L

A

C

D

D

E
|
E

P

A

T

T

G

S

N

A

L

L

D

D

P

P

A

A

T

T

S

T

T

R

A

S

V

I

F

L

Q

E

A

L

L

L

Y

K

Q

D

V

I

C

N

R

R

E

R

Q

L

G

G

V

L

A

T

A

I

V

L

I

L

A

I

T

T

H

H

N

E

M

M

E

D

L

V

A

V

-

K

R

R

Y

I

M

C

D

D

R

C

V

V

V

A

A

V

L

I

K

S

D

N

G

G

H

E

L

L

40:46 (vs. 44:50, 71% identical) binding
E166 (= E171) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

6z4wA Ftse structure from streptococcus pneumoniae in complex with adp (space group p 1) (see paper)
40% identity, 87% coverage: 25:223/228 of query aligns to 20:217/230 of 6z4wA

query
sites
6z4wA

L

L

R

R

G

G

V

V

D

S

L

V

D

S

V

V

Y

Q

P

P

G

G

E

E

V

F

V

A

G

Y

L

I

I

V

G

G

P

P

S

S

G
|
G

S

A

G
|
G

K
|
K

S
|
S

S
x
T

L

F

L

I

H

R

S

S

A

L

G

Y

L

R

L

E

E

V

R

K

P

I

D

D

A

K

G

G

L

S

V

L

A

S

L

V

E

A

G

G

R

F

D

N

C

L

S

V

K

K

L

I

S

K

E

K

R

K

A

D

R

V

T

P

R

L

I

L

R

R

L

R

G

-

T

S

V

V

G

G

F

V

V

V

Y

F

Q

Q

F

D

H

Y

H

K

L

L

P

P

E

K

F

K

S

T

A

V

L

Y

E

E

N

N

V

I

A

A

M

Y

P

A

L

M

T

E

I

V

A

I

G

G

K

E

S

N

R

R

E

N

A

I

E

K

A

R

R

R

A

V

R

M

E

E

L

V

L

L

E

D

S

L

L

V

G

G

L

L

G

K

H

H

R

K

L

V

N

R

H

S

Q

F

P

P

A

N

Q

E

M

L

S

S

G

G

E

E

Q

Q

R

R

V

I

A

A

I

I

A

A

R

R

A

A

L

I

A

V

N

N

R

N

P

P

K

K

L

V

L

L

L

I

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

P

P

A

D

T

N

S

S

T

W

A

E

V

I

F

M

Q

N

A

L

L

L

Y

E

Q

R

V

I

C

N

R

L

E

-

Q

Q

G

G

V

T

A

T

A

I

V

L

I

M

A

A

T

T

H

H

N

N

M

S

E

Q

L

I

A

V

R

N

Y

T

M

L

-

R

D

H

R

R

V

V

V

I

A

A

L

I

K

E

D

N

G

G

H

R

L

V

binding adenosine-5'-diphosphate: G40 (= G45), G42 (= G47), K43 (= K48), S44 (= S49), T45 (≠ S50)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 13, 17, 19

6z67B Ftse structure of streptococcus pneumoniae in complex with amppnp at 2.4 a resolution (see paper)
40% identity, 87% coverage: 25:223/228 of query aligns to 20:217/229 of 6z67B

query
sites
6z67B

L

L

R

R

G

G

V

V

D

S

L

V

D

S

V

V

Y

Q

P

P

G

G

E

E

V

F

V

A

G

Y

L

I

I

V

G

G

P

P

S
|
S

G
|
G

S

A

G
|
G

K
|
K

S
|
S

S
x
T

L

F

L

I

H

R

S

S

A

L

G

Y

L

R

L

E

E

V

R

K

P

I

D

D

A

K

G

G

L

S

V

L

A

S

L

V

E

A

G

G

R

F

D

N

C

L

S

V

K

K

L

I

S

K

E

K

R

K

A

D

R

V

T

P

R

L

I

L

R

R

L

R

G

-

T

S

V

V

G

G

F

V

V

V

Y

F

Q

Q

F

D

H

Y

H

K

L

L

P

P

E

K

F

K

S

T

A

V

L

Y

E

E

N

N

V

I

A

A

M

Y

P

A

L

M

T

E

I

V

A

I

G

G

K

E

S

N

R

R

E

N

A

I

E

K

A

R

R

R

A

V

R

M

E

E

L

V

L

L

E

D

S

L

L

V

G

G

L

L

G

K

H

H

R

K

L

V

N

R

H

S

Q

F

P

P

A

N

Q

E

M

L

S

S

G

G

E

E

Q

Q

R

R

V

I

A

A

I

I

A

A

R

R

A

A

L

I

A

V

N

N

R

N

P

P

K

K

L

V

L

L

L

I

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

P

P

A

D

T

N

S

S

T

W

A

E

V

I

F

M

Q

N

A

L

L

L

Y

E

Q

R

V

I

C

N

R

L

E

-

Q

Q

G

G

V

T

A

T

A

I

V

L

I

M

A

A

T

T

H

H

N

N

M

S

E

Q

L

I

A

V

R

N

Y

T

M

L

-

R

D

H

R

R

V

V

V

I

A

A

L

I

K

E

D

N

G

G

H

R

L

V

binding phosphoaminophosphonic acid-adenylate ester: S39 (= S44), G40 (= G45), G42 (= G47), K43 (= K48), S44 (= S49), T45 (≠ S50)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13, 16, 19

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
37% identity, 96% coverage: 5:223/228 of query aligns to 2:220/344 of 6cvlD

query
sites
6cvlD

V

M

L

I

A

K

L

L

R

S

G

N

L

I

E

T

R

K

V

V

Y
x
F

K

H

T
x
Q

E

G

A

T

G

R

D

T

L
x
I

P

Q

V

A

L

L

R

N

G

N

V

V

D

S

L

L

D

H

V

V

Y

P

P

A

G

G

E

Q

V

I

V

Y

G

G

L

V

I

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

S
x
T

L

L

L

I

H

R

S

C

A

V

G

N

L

L

E

E

R

R

P

P

D

T

A

E

G

G

L

S

V

V

A

L

L

V

E

D

G

G

R

Q

D

E

C

L

S

T

K

T

L

L

S

S

E

E

R

S

A

E

R

L

T

T

R

K

I

A

R

R

L

-

G

R

T

Q

V

I

G

G

F

M

V

I

Y

F

Q

Q

F

H

H

F

H

N

L

L

P

S

E

S

F

R

S

T

A

V

L

F

E

G

N

N

V

V

A

A

M

L

P

P

L

L

T

E

I

L

A

D

G

N

K

T

S

P

R

K

R

D

E

E

A

V

E

K

A

R

R

R

A

V

R

T

E

E

L

L

E

S

S

L

L

V

G

G

L

L

G

G

H

D

R

K

L

H

N

D

H

S

Q

Y

P

P

A

S

Q
x
N

M

L

S
|
S

G

G

E
x
Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

N

S

R

N

P

P

K

K

L

V

L

L

A

C

D

D

E

Q

P

A

T

T

G

S

N

A

L

L

D

D

P

P

A

A

T

T

S

T

T

R

A

S

V

I

F

L

Q

E

A

L

L

L

Y

K

Q

D

V

I

C

N

R

R

E

R

Q

L

G

G

V

L

A

T

A

I

V

L

I

L

A

I

T

T

H
|
H

N

E

M

M

E

D

L

V

A

V

-

K

R

R

Y

I

M

C

D

D

R

C

V

V

V

A

A

V

L

I

K

S

D

N

G

G

H

E

L

L

binding phosphothiophosphoric acid-adenylate ester: F12 (≠ Y15), Q14 (≠ T17), I19 (≠ L22), S41 (= S44), G42 (= G45), A43 (≠ S46), G44 (= G47), K45 (= K48), S46 (= S49), T47 (≠ S50), N141 (≠ Q145), S143 (= S147), Q146 (≠ E150), H200 (= H204)

Query Sequence

>CCNA_02006 CCNA_02006 lipoprotein releasing system ATP-binding protein lolD
MSDPVLALRGLERVYKTEAGDLPVLRGVDLDVYPGEVVGLIGPSGSGKSSLLHSAGLLER
PDAGLVALEGRDCSKLSERARTRIRLGTVGFVYQFHHLLPEFSALENVAMPLTIAGKSRR
EAEARARELLESLGLGHRLNHQPAQMSGGEQQRVAIARALANRPKLLLADEPTGNLDPAT
STAVFQALYQVCREQGVAAVIATHNMELARYMDRVVALKDGHLELQRV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory