SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CCNA_02074 FitnessBrowser__Caulo:CCNA_02074 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

6addA The crystal structure of rv2747 from mycobacterium tuberculosis in complex with coa and nlq (see paper)
33% identity, 62% coverage: 43:138/156 of query aligns to 34:125/168 of 6addA

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6addA

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binding coenzyme a: V75 (≠ M81), A76 (≠ H82), V77 (≠ T83), T82 (≠ R88), G83 (= G89), H84 (≠ R90), G85 (= G91), G87 (= G93), H88 (≠ Q94), T110 (= T116), E112 (≠ A118), F115 (= F121)
binding n~2~-acetyl-l-glutamine: T74 (≠ S80), L109 (≠ E115)

Sites not aligning to the query:

binding coenzyme a: 1, 24, 28
binding n~2~-acetyl-l-glutamine: 27, 28, 30, 147

5yo2A The crystal structure of rv2747 from mycobacterium tuberculosis in complex with acetyl coa and l-arginine (see paper)
33% identity, 62% coverage: 43:138/156 of query aligns to 34:125/168 of 5yo2A

query
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5yo2A

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binding acetyl coenzyme *a: I72 (= I78), R73 (≠ K79), T74 (≠ S80), V75 (≠ M81), V77 (≠ T83), T82 (≠ R88), G83 (= G89), H84 (≠ R90), G85 (= G91), G87 (= G93), H88 (≠ Q94), T110 (= T116), E112 (≠ A118), F115 (= F121), F116 (= F122)
binding arginine: R73 (≠ K79), L109 (≠ E115)

Sites not aligning to the query:

binding acetyl coenzyme *a: 24, 28
binding arginine: 26, 29, 30, 147

5ygeA Arga complexed with acecoa and glutamate (see paper)
35% identity, 59% coverage: 43:134/156 of query aligns to 35:122/169 of 5ygeA

query
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5ygeA

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binding acetyl coenzyme *a: I73 (= I78), V76 (≠ M81), A77 (≠ H82), V78 (≠ T83), T83 (≠ R88), G84 (= G89), H85 (≠ R90), G86 (= G91), G88 (= G93), H89 (≠ Q94), T111 (= T116), E113 (≠ A118), F117 (= F122)
binding glutamic acid: E44 (≠ D52), H64 (≠ K69), R74 (≠ K79)

Sites not aligning to the query:

Query Sequence

>CCNA_02074 FitnessBrowser__Caulo:CCNA_02074
MPKPQLRIIAGDFEDPRIIALLAHHFATMRSTGPEESCHVMPLGTMRQADLDFFAAWDGD
ALAGFGAVKQLGDGHGEIKSMHTAAAYRGRGVGQAVLDHLSTHARALGLQRLSLETGAGD
FFVPARAMYARNGFQTCDPFGDYKPDPNSVFMTRML

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory