SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CCNA_02204 FitnessBrowser__Caulo:CCNA_02204 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

C1DFH7 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Azotobacter vinelandii (strain DJ / ATCC BAA-1303) (see paper)
63% identity, 100% coverage: 1:339/339 of query aligns to 1:338/338 of C1DFH7

query
sites
C1DFH7

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D190 (= D191) binding Mg(2+)
E226 (= E227) binding Mg(2+)
E230 (= E231) binding Mg(2+)

4xiyA Crystal structure of ketol-acid reductoisomerase from azotobacter (see paper)
63% identity, 97% coverage: 1:329/339 of query aligns to 1:328/328 of 4xiyA

query
sites
4xiyA

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active site: K130 (= K131), D190 (= D191), E194 (= E195)
binding fe (iii) ion: D190 (= D191), E194 (= E195)
binding magnesium ion: E226 (= E227), E230 (= E231)

8upqB Campylobacter jejuni ketol-acid reductoisomerase in complex with 2,3- dihydroxy-3-isovalerate.
62% identity, 100% coverage: 1:339/339 of query aligns to 2:339/342 of 8upqB

query
sites
8upqB

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binding magnesium ion: D191 (= D191), E195 (= E195)
binding (2R)-2,3-dihydroxy-3-methylbutanoic acid: A132 (≠ G132), P133 (= P133), D191 (= D191), E195 (= E195), E231 (= E231), S252 (= S252), A255 (= A255)

7rduA Crystal structure of campylobacter jejuni keto said reductoisomerase in complex with magnesium and oxidixized and reduced NADPH
62% identity, 97% coverage: 1:329/339 of query aligns to 2:329/329 of 7rduA

query
sites
7rduA

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K

K

A

K

E

A

M

M

K

K

R

G

V

V

L

L

E

K

D

D

I

I

Q

Q

S

N

G

G

K

V

F

F

V

A

R

K

D

D

F

F

M

I

L

L

E

E

N

R

A

R

V

A

G

G

Q

F

P

A

S

R

F

M

K

H

A

A

T

E

R

R

R

K

R

N

S

M

A

N

E

D

H

S

Q

L

I

I

E

E

E

K

V

T

G

G

A

R

R

N

L

L

R

R

G

A

M

M

P

P

W

W

I

I

A

S

binding nadp nicotinamide-adenine-dinucleotide phosphate: G24 (= G23), G26 (= G25), S27 (= S26), Q28 (= Q27), R48 (= R48), S51 (= S51), S53 (≠ T53), A81 (= A81), P82 (= P82), D83 (= D83), I89 (= I89), A107 (= A107), H108 (= H108), P130 (= P130), K131 (= K131), A132 (≠ G132)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G24 (= G23), F25 (≠ Y24), G26 (= G25), S27 (= S26), Q28 (= Q27), S51 (= S51), S53 (≠ T53), L80 (= L80), P82 (= P82), D83 (= D83), I89 (= I89), A107 (= A107), H108 (= H108)

8uppA Campylobacter jejuni ketol-acid reductoisomerase in complex with NADPH and hoe704
62% identity, 97% coverage: 1:328/339 of query aligns to 1:327/327 of 8uppA

query
sites
8uppA

M

I

R

T

V

V

Y

Y

D

D

R

K

D

D

A

C

D

D

L

L

A

N

R

L

I

I

L

K

D

S

R

K

K

K

I

V

A

A

I

I

V

I

G

G

Y
x
F

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

R

R

D

D

S

N

G

G

I

V

K

-

N

N

V

V

A

T

V

I

A

G

L

L

R
|
R

A

E

G

G

S
|
S

P

V

T

S

A

A

K

V

K

K

A

A

E

K

G

N

E

A

G

G

L

F

K

E

V

V

M

M

T

S

V

V

A

S

E

E

A

A

A

S

A

K

W

I

A

A

D

D

L

V

I

I

M

M

I

I

L
|
L

A

A

P
|
P

D
|
D

E

E

H
x
I

Q
|
Q

A

A

A

D

I
|
I

Y

F

K

N

N

V

E

E

I

I

A

K

L

P

N

N

I

L

R

S

D

E

G

G

A

K

A

A

L

I

L

A

F

F

A

A

H
|
H

G

G

L

F

N

N

V

I

H

H

F

Y

G

G

L

Q

I

I

E

V

P

V

K

P

D

K

T

G

I

V

D

D

V

V

L

I

M

M

V

I

A

A

P

P

K

K

G
x
A

P
|
P

G

G

H

H

T

T

V

V

R

R

G

N

E

E

Y

F

Q

T

K

L

G

G

V

T

P

P

C

C

L

L

I

I

A

A

V

I

H

H

H

Q

N

D

A

E

T

S

G

K

N

N

A

A

L

K

D

N

L

L

G

A

L

L

A

S

Y

Y

A

A

S

S

A

A

I

I

G

G

R

R

S

T

G

G

I

I

E

E

T

T

N

T

F

F

R

K

E

A

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

L

V

S

E

A

L

L

V

I

R

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E
|
E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

M

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

N

R

Y

Y

S

S

I
|
I

S
|
S

N

N

T

T

A
|
A

E

E

Y

Y

G

G

E

D

Y

Y

V

I

T

T

G

G

P

P

R

K

I

I

T

T

P

E

E

E

T

T

K

K

A

K

E

A

M

M

K

K

R

G

V

V

L

L

E

K

D

D

I

I

Q

Q

S

N

G

G

K

V

F

F

V

A

R

K

D

D

F

F

M

I

L

L

E

E

N

R

A

R

V

A

G

G

Q

F

P

A

S

R

F

M

K

H

A

A

T

E

R

R

R

K

R

N

S

M

A

N

E

D

H

S

Q

L

I

I

E

E

E

K

V

T

G

G

A

R

R

N

L

L

R

R

G

A

M

M

P

P

W

W

I

I

binding magnesium ion: D190 (= D191), E194 (= E195)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F24 (≠ Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), R47 (= R48), S50 (= S51), L79 (= L80), P81 (= P82), D82 (= D83), I84 (≠ H85), Q85 (= Q86), I88 (= I89), H107 (= H108), P132 (= P133), I250 (= I251), S251 (= S252)
binding (2R)-(dimethylphosphoryl)(hydroxy)acetic acid: A131 (≠ G132), P132 (= P133), D190 (= D191), E194 (= E195), E230 (= E231), I250 (= I251), S251 (= S252), A254 (= A255)

8sxdA Campylobacter jejuni keto-acid reductoisomerase in complex with intermediate and NADP+
62% identity, 97% coverage: 1:328/339 of query aligns to 1:327/327 of 8sxdA

query
sites
8sxdA

M

I

R

T

V

V

Y

Y

D

D

R

K

D

D

A

C

D

D

L

L

A

N

R

L

I

I

L

K

D

S

R

K

K

K

I

V

A

A

I

I

V

I

G
|
G

Y
x
F

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

R

R

D

D

S

N

G

G

I

V

K

-

N

N

V

V

A

T

V

I

A

G

L

L

R

R

A

E

G

G

S
|
S

P

V

T
x
S

A

A

K

V

K

K

A

A

E

K

G

N

E

A

G

G

L

F

K

E

V

V

M

M

T

S

V

V

A

S

E

E

A

A

A

S

A

K

W

I

A

A

D

D

L

V

I

I

M

M

I

I

L
|
L

A
|
A

P
|
P

D
|
D

E

E

H

I

Q

Q

A

A

A

D

I

I

Y

F

K

N

N

V

E

E

I

I

A

K

L

P

N

N

I

L

R

S

D

E

G

G

A

K

A

A

L

I

L

A

F

F

A

A

H

H

G

G

L

F

N

N

V

I

H

H

F

Y

G

G

L

Q

I

I

E

V

P

V

K

P

D

K

T

G

I

V

D

D

V

V

L

I

M

M

V

I

A

A

P
|
P

K

K

G

A

P

P

G

G

H

H

T

T

V

V

R

R

G

N

E

E

Y

F

Q

T

K

L

G

G

V

T

P

P

C

C

L

L

I

I

A

A

V

I

H

H

H

Q

N

D

A

E

T

S

G

K

N

N

A

A

L

K

D

N

L

L

G

A

L

L

A

S

Y

Y

A

A

S

S

A

A

I

I

G

G

R

R

S

T

G

G

I

I

E

E

T

T

N

T

F

F

R

K

E

A

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

L

V

S

E

A

L

L

V

I

R

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

M

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

N

R

Y

Y

S

S

I

I

S

S

N

N

T

T

A

A

E

E

Y

Y

G

G

E

D

Y

Y

V

I

T

T

G

G

P

P

R

K

I

I

T

T

P

E

E

E

T

T

K

K

A

K

E

A

M

M

K

K

R

G

V

V

L

L

E

K

D

D

I

I

Q

Q

S

N

G

G

K

V

F

F

V

A

R

K

D

D

F

F

M

I

L

L

E

E

N

R

A

R

V

A

G

G

Q

F

P

A

S

R

F

M

K

H

A

A

T

E

R

R

R

K

R

N

S

M

A

N

E

D

H

S

Q

L

I

I

E

E

E

K

V

T

G

G

A

R

R

N

L

L

R

R

G

A

M

M

P

P

W

W

I

I

binding magnesium ion: D190 (= D191), E194 (= E195)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G23 (= G23), F24 (≠ Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), S50 (= S51), S52 (≠ T53), L79 (= L80), A80 (= A81), P81 (= P82), D82 (= D83), P129 (= P130)
binding 3-hydroxy-3-methyl-2-oxobutanoic acid: D190 (= D191), E194 (= E195)

8swmA Crystal structure of campylobacter jejuni ketol-acid reductoisomerase in complex with 2-acetolactate
62% identity, 96% coverage: 4:327/339 of query aligns to 1:323/323 of 8swmA

query
sites
8swmA

Y

Y

D

D

R

K

D

D

A

C

D

D

L

L

A

N

R

L

I

I

L

K

D

S

R

K

K

K

I

V

A

A

I

I

V

I

G

G

Y

F

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

R

R

D

D

S

N

G

G

I

V

K

-

N

N

V

V

A

T

V

I

A

G

L

L

R

R

A

E

G

G

S

S

P

V

T

S

A

A

K

V

K

K

A

A

E

K

G

N

E

A

G

G

L

F

K

E

V

V

M

M

T

S

V

V

A

S

E

E

A

A

A

S

A

K

W

I

A

A

D

D

L

V

I

I

M

M

I

I

L

L

A

A

P

P

D

D

E

E

H

I

Q

Q

A

A

A

D

I

I

Y

F

K

N

N

V

E

E

I

I

A

K

L

P

N

N

I

L

R

S

D

E

G

G

A

K

A

A

L

I

L

A

F

F

A

A

H

H

G

G

L

F

N

N

V

I

H

H

F

Y

G

G

L

Q

I

I

E

V

P

V

K

P

D

K

T

G

I

V

D

D

V

V

L

I

M

M

V

I

A

A

P

P

K

K

G

A

P

P

G

G

H

H

T

T

V

V

R

R

G

N

E

E

Y

F

Q

T

K

L

G

G

V

T

P

P

C

C

L

L

I

I

A

A

V

I

H

H

H

Q

N

D

A

E

T

S

G

K

N

N

A

A

L

K

D

N

L

L

G

A

L

L

A

S

Y

Y

A

A

S

S

A

A

I

I

G

G

R

R

S

T

G

G

I

I

E

E

T

T

N

T

F

F

R

K

E

A

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

L

V

S

E

A

L

L

V

I

R

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

M

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

N

R

Y

Y

S

S

I

I

S

S

N

N

T

T

A

A

E

E

Y

Y

G

G

E

D

Y

Y

V

I

T

T

G

G

P

P

R

K

I

I

T

T

P

E

E

E

T

T

K

K

A

K

E

A

M

M

K

K

R

G

V

V

L

L

E

K

D

D

I

I

Q

Q

S

N

G

G

K

V

F

F

V

A

R

K

D

D

F

F

M

I

L

L

E

E

N

R

A

R

V

A

G

G

Q

F

P

A

S

R

F

M

K

H

A

A

T

E

R

R

R

K

R

N

S

M

A

N

E

D

H

S

Q

L

I

I

E

E

E

K

V

T

G

G

A

R

R

N

L

L

R

R

G

A

M

M

P

P

W

W

binding magnesium ion: D187 (= D191), E191 (= E195)
binding (2S)-2-hydroxy-2-methyl-3-oxobutanoic acid: D187 (= D191), E191 (= E195)

8cy8A Apo form cryo-em structure of campylobacter jejune ketol-acid reductoisommerase crosslinked by glutaraldehyde
59% identity, 97% coverage: 1:329/339 of query aligns to 1:312/312 of 8cy8A

query
sites
8cy8A

M

I

R

T

V

V

Y

Y

D

D

R

K

D

D

A

C

D

D

L

L

A

N

R

L

I

I

L

K

D

S

R

K

K

K

I

V

A

A

I

I

V

I

G

G

Y

F

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

A

L

M

N

N

L

L

R

R

D

D

S

N

G

G

I

V

K

-

N

N

V

V

A

T

V

I

A

G

L

L

R

R

A

-

G

-

S

-

P

-

T

-

A

-

K

-

A

-

E

-

G

N

E

A

G

G

L

F

K

E

V

V

M

M

T

S

V

V

A

S

E

E

A

A

A

S

A

K

W

I

A

A

D

D

L

V

I

I

M

M

I

I

L

L

A

A

P

P

D

D

E

E

H

I

Q

Q

A

A

A

D

I

I

Y

F

K

N

N

V

E

E

I

I

A

K

L

P

N

N

I

L

R

S

D

E

G

G

A

K

A

A

L

I

L

A

F

F

A

A

H

H

G

G

L

F

N

N

V

I

H

H

F

Y

G

G

L

Q

I

I

E

V

P

V

K

P

D

K

T

G

I

V

D

D

V

V

L

I

M

M

V

I

A

A

P

P

K

K

G

A

P

P

G

G

H

H

T

T

V

V

R

R

G

N

E

E

Y

-

Q

-

K

-

G

-

V

T

P

P

C

C

L

L

I

I

A

A

V

I

H

H

H

Q

N

D

A

E

T

S

G

K

N

N

A

A

L

K

D

N

L

L

G

A

L

L

A

S

Y

Y

A

A

S

S

A

A

I

I

G

G

R

R

S

T

G

G

I

I

E

E

T

T

N

T

F

F

R

K

E

A

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L
|
L

C

C

G

G

T

L

V

S

E

A

L

L

V

I

R

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

M

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

N

R

Y

Y

S

S

I

I

S

S

N

N

T

T

A

A

E

E

Y

Y

G

G

E

D

Y

Y

V

I

T

T

G

G

P

P

R

K

I

I

T

T

P

E

E

E

T

T

K

K

A

K

E

A

M

M

K

K

R

G

V

V

L

L

E

K

D

D

I

I

Q

Q

S

N

G

G

K

V

F

F

V

A

R

K

D

D

F

F

M

I

L

L

E

E

N

R

A

R

V

A

G

G

Q

F

P

A

S

R

F

M

K

H

A

A

T

E

R

R

R

K

R

N

S

M

A

N

E

D

H

S

Q

L

I

I

E

E

E

K

V

T

G

G

A

R

R

N

L

L

R

R

G

A

M

M

P

P

W

W

I

I

A

S

binding pentanedial: D174 (= D191), E178 (= E195), L182 (= L199)

C8WR67 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Alicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / BCRC 14685 / JCM 5260 / KCTC 1825 / NBRC 15652 / NCIMB 11725 / NRRL B-14509 / 104-IA) (Bacillus acidocaldarius) (see paper)
60% identity, 99% coverage: 2:335/339 of query aligns to 3:334/344 of C8WR67

query
sites
C8WR67

R

K

V

I

Y

Y

D

D

R

A

D

D

A

I

D

S

L

I

A

Q

R

P

I

L

L

A

D

D

R

K

K

R

I

I

A

A

I

V

V

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

Q

N

N

L

L

R

R

D

D

S

S

G

G

I

F

K

-

N

D

V

V

A

V

V

I

A

G

L

L

R
|
R

A

P

G

G

S

S

P
x
S

T

W

A

A

K

K

K

-

A

A

E

E

G

A

E

D

G

G

L

F

K

R

V

V

M

M

T

A

V

V

A

G

E

E

A

A

A

V

A

E

W

E

A

S

D

D

L

V

I

I

M

M

I

I

L

L

A

L

P

P

D
|
D

E
|
E

H
x
R

Q
|
Q

A

P

A

A

I

V

Y

Y

K

E

N

R

E

E

I

I

A

R

L

P

N

Y

I

L

R

T

D

A

G

G

A

K

A

A

L

L

L

A

F

F

A

A

H

H

G

G

L

F

N

N

V

I

H

H

F

F

G

S

L

Q

I

I

E

Q

P

P

K

P

D

K

T

D

I

V

D

D

V

V

L

F

M

M

V

V

A

A

P

P

K

K

G

G

P

P

G
|
G

H

H

T

L

V

V

R

R

G

R

E

V

Y

Y

Q

E

K

A

G

G

V

V

P

P

C

A

L

L

I

I

A

A

V

V

H

H

H

Q

N

D

A

A

T

S

G

G

N

Q

A

A

L

K

D

D

L

L

G

A

L

L

A

A

Y

Y

A

A

S

R

A

G

I

I

G

G

G

A

G

G

R

R

S

A

G

G

I

I

I

L

E

T

T

T

N

T

F

F

R

R

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

L

V

S

E

A

L

L

V

I

R

K

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

Q

P

P

E

E

M

I

A

A

Y

Y

F

F

E
|
E

C

C

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

M

I

Y

Y

E

E

G

G

I

L

A

E

N

Y

M

M

N

R

Y

Y

S

S

I

I

S

S

N

D

T

T

A

A

E

Q

Y

W

G

G

E

D

Y

F

V

T

T

S

G

G

P

P

R

R

I

I

T

N

P

E

E

E

T

T

K

K

A

K

E

E

M

M

K

R

R

R

V

I

L

L

E

A

D

D

I

I

Q

Q

S

S

G

G

K

A

F

F

V

A

R

K

D

S

F

W

M

I

L

L

E

E

N

N

A

Q

V

A

G

N

Q

R

P

P

S

M

F

F

K

N

A

A

T

I

R

N

R

R

S

E

A

L

E

E

H

H

Q

P

I

I

E

E

E

V

V

V

G

G

A

R

R

K

L

L

R

R

G

S

M

M

P

P

W

F

I

I

A

K

K

A

N

K

K

R

L

P

V

G

D

D

YGSQ 25:28 (= YGSQ 24:27) binding NADP(+)
R48 (= R48) binding NADP(+); mutation to P: Inversion of the cofactor specificity from NADPH to NADH.
S52 (≠ P52) binding NADP(+); mutation to D: Inversion of the cofactor specificity from NADPH to NADH.
DERQ 82:85 (≠ DEHQ 83:86) binding NADP(+)
G133 (= G134) binding NADP(+)
D190 (= D191) binding Mg(2+)
E194 (= E195) binding Mg(2+)
E226 (= E227) binding Mg(2+)

4tskA Ketol-acid reductoisomerase from alicyclobacillus acidocaldarius (see paper)
60% identity, 99% coverage: 2:335/339 of query aligns to 2:333/333 of 4tskA

query
sites
4tskA

R

K

V

I

Y

Y

D

D

R

A

D

D

A

I

D

S

L

I

A

Q

R

P

I

L

L

A

D

D

R

K

K

R

I

I

A

A

I

V

V

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

H

A

A

L

Q

N

N

L

L

R

R

D

D

S

S

G

G

I

F

K

-

N

D

V

V

A

V

V

I

A

G

L
|
L

R
|
R

A

P

G

G

S

S

P
x
S

T

W

A

A

K

K

K

-

A

A

E

E

G

A

E

D

G

G

L

F

K

R

V

V

M

M

T

A

V

V

A

G

E

E

A

A

A

V

A

E

W

E

A

S

D

D

L

V

I

I

M

M

I

I

L
|
L

A
x
L

P
|
P

D
|
D

E

E

H

R

Q

Q

A

P

A

A

I

V

Y

Y

K

E

N

R

E

E

I

I

A

R

L

P

N

Y

I

L

R

T

D

A

G

G

A

K

A

A

L

L

L

A

F

F

A

A

H
|
H

G

G

L

F

N

N

V

I

H

H

F

F

G

S

L

Q

I

I

E

Q

P

P

K

P

D

K

T

D

I

V

D

D

V

V

L

F

M

M

V

V

A

A

P

P

K
|
K

G

G

P
|
P

G

G

H

H

T

L

V

V

R

R

G

R

E

V

Y

Y

Q

E

K

A

G

G

V

V

P

P

C

A

L

L

I

I

A

A

V

V

H

H

H

Q

N

D

A

A

T

S

G

G

N

Q

A

A

L

K

D

D

L

L

G

A

L

L

A

A

Y

Y

A

A

S

R

A

G

I

I

G

G

G

A

G

G

R

R

S

A

G

G

I

I

I

L

E

T

T

T

N

T

F

F

R

R

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

L

V

S

E

A

L

L

V

I

R

K

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

Q

P

P

E

E

M

I

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

L

M

K

K

L

L

I

I

V

V

D

D

L

L

M

I

Y

Y

E

E

G

G

I

L

A

E

N

Y

M

M

N
x
R

Y
|
Y

S

S

I
|
I

S

S

N
x
D

T

T

A

A

E
x
Q

Y

W

G

G

E

D

Y

F

V

T

T

S

G

G

P

P

R

R

I

I

T

N

P

E

E

E

T

T

K

K

A

K

E

E

M

M

K

R

R

R

V

I

L

L

E

A

D

D

I

I

Q

Q

S

S

G

G

K

A

F

F

V

A

R

K

D

S

F

W

M

I

L

L

E

E

N

N

A

Q

V

A

G

N

Q

R

P

P

S

M

F

F

K

N

A

A

T

I

R

N

R

R

S

E

A

L

E

E

H

H

Q

P

I

I

E

E

E

V

V

V

G

G

A

R

R

K

L

L

R

R

G

S

M

M

P

P

W

F

I

I

A

K

K

A

N

K

K

R

L

P

V

G

D

D

active site: K129 (= K131), D189 (= D191), E193 (= E195)
binding magnesium ion: D189 (= D191), D189 (= D191), E193 (= E195), E193 (= E195), R246 (≠ N248), Y247 (= Y249), I249 (= I251), D251 (≠ N253), Q254 (≠ E256)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: Y24 (= Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), L46 (= L47), R47 (= R48), S51 (≠ P52), L78 (= L80), L79 (≠ A81), P80 (= P82), D81 (= D83), H106 (= H108), P131 (= P133)

P9WKJ7 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
56% identity, 97% coverage: 1:330/339 of query aligns to 3:331/337 of P9WKJ7

query
sites
P9WKJ7

M

L

R

E

V

M

Y

F

Y

Y

D

D

R

D

D

D

A

A

D

D

L

L

A

S

R

I

I

I

L

Q

D

G

R

R

K

K

I

V

A

G

I

V

V

I

G

G

Y

Y

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

S

L

L

N

S

L

L

R

R

D

D

S

S

G

G

I

V

K

Q

N

-

V

V

A

R

V

V

A

G

L

L

R

K

A

Q

G

G

S

S

P

R

T

S

A

R

K

P

K

K

A

V

E

E

G

E

E

Q

G

G

L

L

K

D

V

V

M

D

T

T

V

P

A

A

E

E

A

V

A

A

A

K

W

W

A

A

D

D

L

V

I

V

M

M

I

V

L

L

A

A

P

P

D

D

E

T

H

A

Q

Q

A

A

A

E

I

I

Y

F

K

A

N

G

E

D

I

I

A

E

L

P

N

N

I

L

R

K

D

P

G

G

A

D

A

A

L

L

L

F

F

F

A

G

H

H

G

G

L

L

N

N

V

V

H

H

F

F

G

G

L

L

I

I

E

K

P

P

K

P

D

A

T

D

I

V

D

A

V

V

L

A

M

M

V

V

A

A

P

P

K

K

G

G

P

P

G

G

H

H

T

L

V

V

R

R

G

R

E

Q

Y

F

Q

V

K

D

G

G

G

K

G

G

V

V

P

P

C

C

L

L

I

V

A

A

V

V

H

E

H

Q

N

D

A

P

T

R

G

G

N

D

A

G

L

L

D

A

L

L

G

A

L

L

A

S

Y

Y

A

A

S

K

A

A

I

I

G

G

G

T

R

R

S

A

G

G

I

V

I

I

E

K

T

T

N

T

F

F

R

K

E

D

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

T

V

V

L

L

C

C

G

G

T

T

V

E

E

E

L

L

V

V

R

K

A

A

G

G

F

F

E

E

T

V

L

M

V

V

E

E

A

A

G

G

Y

Y

A

P

P

A

E

E

M

L

A

A

Y

Y

F

F

E
|
E

C

V

L

L

H

H

E
|
E

L

L

K

K

L

L

I

I

V

V

D

D

L

L

M

M

Y

Y

E

E

G

G

I

L

A

A

N

R

M

M

N

Y

Y

Y

S

S

I

V

S

S

N

D

T

T

A

A

E

E

Y

F

G

G

E

G

Y

Y

V

L

T

S

G

G

P

P

R

R

I

V

I

I

T

D

P

A

E

G

T

T

K

K

A

E

E

R

M

M

K

R

R

D

V

I

L

L

E

R

D

E

I

I

Q

Q

S

D

G

G

K

S

F

F

V

V

R

H

D

K

F

L

M

V

L

A

E

D

N

V

A

E

V

G

G

G

Q

N

P

K

S

Q

F

L

K

E

A

E

T

L

R

R

R

Q

S

N

A

A

E

E

H

H

Q

P

I

I

E

E

E

V

V

V

G

G

A

K

R

K

L

L

R

R

G

D

M

L

M

M

P

S

W

W

I

V

A

D

K

R

D192 (= D191) binding Mg(2+); binding Mg(2+)
E196 (= E195) binding Mg(2+)
E228 (= E227) binding Mg(2+)
E232 (= E231) binding Mg(2+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4ypoA Crystal structure of mycobacterium tuberculosis ketol-acid reductoisomerase in complex with mg2+ (see paper)
57% identity, 96% coverage: 3:328/339 of query aligns to 1:325/325 of 4ypoA

query
sites
4ypoA

V

M

Y

F

Y

Y

D

D

R

D

D

D

A

A

D

D

L

L

A

S

R

I

I

I

L

Q

D

G

R

R

K

K

I

V

A

G

I

V

V

I

G

G

Y

Y

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

S

L

L

N

S

L

L

R

R

D

D

S

S

G

G

I

V

K

Q

N

-

V

V

A

R

V

V

A

G

L

L

R

K

A

Q

G

G

S

S

P

R

T

S

A

R

K

P

K

K

A

V

E

E

G

E

E

Q

G

G

L

L

K

D

V

V

M

D

T

T

V

P

A

A

E

E

A

V

A

A

A

K

W

W

A

A

D

D

L

V

I

V

M

M

I

V

L

L

A

A

P

P

D

D

E

T

H

A

Q

Q

A

A

A

E

I

I

Y

F

K

A

N

G

E

D

I

I

A

E

L

P

N

N

I

L

R

K

D

P

G

G

A

D

A

A

L

L

L

F

F

F

A

G

H

H

G

G

L

L

N

N

V

V

H

H

F

F

G

G

L

L

I

I

E

K

P

P

K

P

D

A

T

D

I

V

D

A

V

V

L

A

M

M

V

V

A

A

P

P

K
|
K

G

G

P

P

G

G

H

H

T

L

V

V

R

R

G

R

E

Q

Y

F

Q

V

K

D

G

G

G

K

G

G

V

V

P

P

C

C

L

L

I

V

A

A

V

V

H

E

H

Q

N

D

A

P

T

R

G

G

N

D

A

G

L

L

D

A

L

L

G

A

L

L

A

S

Y

Y

A

A

S

K

A

A

I

I

G

G

G

T

R

R

S

A

G

G

I

V

I

I

E

K

T

T

N

T

F

F

R

K

E

D

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

T

V

V

L

L

C

C

G

G

T

T

V

E

E

E

L

L

V

V

R

K

A

A

G

G

F

F

E

E

T

V

L

M

V

V

E

E

A

A

G

G

Y

Y

A

P

P

A

E

E

M

L

A

A

Y

Y

F

F

E
|
E

C

V

L

L

H

H

E
|
E

L

L

K

K

L

L

I

I

V

V

D

D

L

L

M

M

Y

Y

E

E

G

G

I

L

A

A

N

R

M

M

N

Y

Y

Y

S

S

I

V

S

S

N

D

T

T

A

A

E

E

Y

F

G

G

E

G

Y

Y

V

L

T

S

G

G

P

P

R

R

I

V

I

I

T

D

P

A

E

G

T

T

K

K

A

E

E

R

M

M

K

R

R

D

V

I

L

L

E

R

D

E

I

I

Q

Q

S

D

G

G

K

S

F

F

V

V

R

H

D

K

F

L

M

V

L

A

E

D

N

V

A

E

V

G

G

G

Q

N

P

K

S

Q

F

L

K

E

A

E

T

L

R

R

R

Q

S

N

A

A

E

E

H

H

Q

P

I

I

E

E

E

V

V

V

G

G

A

K

R

K

L

L

R

R

G

D

M

L

M

M

P

S

W

W

I

V

active site: K128 (= K131), D188 (= D191), E192 (= E195)
binding magnesium ion: D188 (= D191), E192 (= E195), E224 (= E227), E228 (= E231)

8ep7C Crystal structure of the ketol-acid reductoisomerase from bacillus anthracis in complex with NADP
57% identity, 97% coverage: 1:328/339 of query aligns to 1:326/328 of 8ep7C

query
sites
8ep7C

M

M

R

K

V

T

Y

Y

D

E

R

Q

D

D

A

A

D

N

L

V

A

G

R

L

I

L

L

Q

D

G

R

K

K

T

I

V

A

A

I

V

V

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

Q

A

A

L

Q

N

N

L

L

R

R

D

D

S

S

G

G

I

V

K

E

N

-

V

V

A

V

V

V

A

G

L

V

R
|
R

A

P

G

G

S

K

P

-

T
x
S

A

F

K

E

K

V

A

A

E

K

G

A

E

D

G

G

L

F

K

E

V

V

M

M

T

S

V

V

A

S

E

E

A

A

A

V

A

R

W

T

A

A

D

Q

L

V

I

V

M

Q

I

M

L
|
L

A
x
L

P
|
P

D
|
D

E

E

H
x
Q

Q

Q

A

A

A

H

I
x
V

Y

Y

K

K

N

A

E

E

I

V

A

E

L

E

N

N

I

L

R

R

D

E

G

G

A

Q

A

M

L

L

F

F

A

S

H
|
H

G

G

L

F

N

N

V

I

H

H

F

F

G

G

L

Q

I

I

E

N

P

P

K

P

D

S

T

Y

I

V

D

D

V

V

L

A

M

M

V

V

A

A

P

P

K

K

G

S

P

P

G

G

H

H

T

L

V

V

R

R

G

R

E

V

Y

F

Q

Q

K

E

G

G

G

N

G

G

V

V

P

P

C

A

L

L

I

V

A

A

V

V

H

H

H

Q

N

D

A

A

T

T

G

G

N

T

A

A

L

L

D

H

L

V

G

A

L

L

A

A

Y

Y

A

A

S

K

A

G

I

V

G

G

G

C

G

T

R

R

S

A

G

G

I

V

I

I

E

E

T

T

N

T

F

F

R

Q

E

E

C

T

E

E

T

T

D

D

L

L

F

F

G

G

E

E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

V

V

T

E

A

L

L

V

V

R

K

A

A

G

G

F

F

E

E

T

T

L

L

V

T

E

E

A

G

G

G

Y

Y

A

R

P

P

E

E

M

I

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

L

L

K

K

L

L

I

I

V

V

D

D

L

L

M

M

Y

Y

E

E

G

G

I

L

A

T

N

N

M

M

N

R

Y

H

S

S

I

I

S

S

N

D

T

T

A

A

E

E

Y

F

G

G

E

D

Y

Y

V

V

T

T

G

G

P

S

R

R

I

I

I

V

T

T

P

D

E

E

T

T

K

K

A

K

E

E

M

M

K

K

R

R

V

V

L

L

E

T

D

E

I

I

Q

Q

S

Q

G

G

K

E

F

F

V

A

R

K

D

K

F

W

M

I

L

L

E

E

N

N

A

Q

V

A

G

G

Q

R

P

P

S

T

F

Y

K

N

A

A

T

M

R

K

R

A

S

E

A

Q

E

N

H

H

Q

Q

I

L

E

E

E

K

V

V

G

G

A

E

R

E

L

L

R

R

G

E

M

M

P

S

W

W

I

I

binding nadp nicotinamide-adenine-dinucleotide phosphate: Y24 (= Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), R47 (= R48), S51 (≠ T53), L78 (= L80), L79 (≠ A81), P80 (= P82), D81 (= D83), Q83 (≠ H85), V87 (≠ I89), H106 (= H108)

6jx2B Crystal structure of ketol-acid reductoisomerase from corynebacterium glutamicum (see paper)
60% identity, 97% coverage: 1:328/339 of query aligns to 1:327/327 of 6jx2B

query
sites
6jx2B

M

I

R

E

V

L

Y

L

Y

Y

D

D

R

A

D

D

A

A

D

D

L

L

A

S

R

L

I

I

L

Q

D

G

R

R

K

K

I

V

A

A

I

I

V

V

G

G

Y

Y

G

G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

S

L

Q

N

N

L

L

R

R

D

D

S

S

G

G

I

V

K

E

N

-

V

V

A

V

V

I

A

G

L

L

R

R

A

E

G

G

S

S

P

K

T

S

A

A

K

E

K

K

A

A

E

K

G

E

E

A

G

G

L

F

K

E

V

V

M

K

T

T

V

T

A

A

E

E

A

A

W

W

A
|
A

D

D

L

V

I

I

M

M

I

L

L

L

A

A

P

P

D

D

E

T

H

S

Q

Q

A

A

A

E

I

I

Y

F

K

T

N

N

E

D

I

I

A

E

L

P

N

N

I

L

R
x
N

D

A

G

G

A
x
D

A

A

L

L

F

F

A

G

H

H

G

G

L

L

N

N

V

I

H

H

F

F

G

D

L

L

I

I

E

K

P

P

K

A

D

D

T

D

I

I

D

I

V

V

L

G

M

M

V

V

A

A

P

P

K

K

G

G

P

P

G

G

H

H

T

L

V

V

R

R

G

R

E

Q

Y

F

Q

V

K

D

G

G

G

K

G

G

V

V

P

P

C

C

L

L

I

I

A

A

V

V

H

D

H

Q

N

D

A

P

T

T

G

G

N

T

A

A

L

Q

D

A

L

L

G

T

L

L

A

S

Y

Y

A

A

S

A

A

A

I

I

G

G

G

A

R

R

S

A

G

G

I

V

I

I

E

P

T

T

N

T

F

F

R

E

E

A

E

E

C

T

E

V

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

T

V

E

E

E

L

L

V

V

R

K

A

V

G

G

F

F

E

E

T

V

L

L

V

T

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E
|
E

C

V

L

L

H

H

E
|
E

L

L

K

K

L

L

I

I

V

V

D

D

L

L

M

M

Y

F

E

E

G

G

I

I

A

S

N

N

M

M

N

N

Y

Y

S

S

I

V

S

S

N

D

T

T

A

A

E

E

Y

F

G

G

E

G

Y

Y

V

L

T

S

G

G

P

P

R

R

I

V

I

I

T

D

P

A

E

D

T

T

K

K

A

S

E

R

M

M

K

K

R

D

V

I

L

L

E

T

D

D

I

I

Q

Q

S

D

G

G

K

T

F

F

V

T

R

K

D

R

F

L

M

I

L

A

E

N

N

V

A

E

V

N

G

G

Q

N

P

T

S

E

F

L

K

E

A

G

T

L

R

R

R

A

R

S

S

Y

A

N

E

N

H

H

Q

P

I

I

E

E

V

T

G

G

A

A

R

K

L

L

R

R

G

D

M

L

M

M

P

S

W

W

I

V

binding magnesium ion: A73 (= A74), N98 (≠ R99), D101 (≠ A102), D190 (= D191), D190 (= D191), E194 (= E195), E194 (= E195), E226 (= E227), E230 (= E231)

8k32C The complex structure of slkari with nadh at 2.12-angstrom resolution
58% identity, 96% coverage: 2:328/339 of query aligns to 2:332/333 of 8k32C

query
sites
8k32C

R

R

V

M

Y

Y

D

D

R

A

D

D

A

A

D

N

L

L

A

D

R

L

I

L

L

K

D

G

R

K

K

T

I

I

A

A

I

V

V

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

H

H

A

A

H

Q

A

A

L

Q

N

N

L

L

R

H

D

D

S

S

G

G

I

L

K

E

N

-

V

V

A

V

V

V

A

G

L

L

R
|
R

A

K

G

P

S

E

P

D

T

D

A

F

K

T

A

A

E
|
E

-

W

-

N

-

Q

-

V

G

A

E

D

G

G

L

L

K

T

V

P

M

L

T

P

V

V

A

D

E

E

A

A

A

R

W

A

A

A

D

Q

L

I

I

I

M

Q

I

I

L
|
L

A
x
V

P
|
P

D
|
D

E

D

H

I

Q

Q

A

A

A

K

I
x
V

Y

Y

K

R

N

E

E

K

I

I

A

E

L

P

N

Y

I

L

R

N

D

E

G

G

A

D

A

A

L

L

L

G

F

F

A
x
S

H
|
H

G

G

L

F

N

N

V

I

H

H

F

F

G

G

L

Q

I

I

E

V

P

P

K

P

D

P

T

S

I

V

D

D

V

V

L

F

M

M

V

V

A

A

P

P

K

K

G

S

P

P

G

G

H

H

T

L

V

V

R

R

G

R

E

M

Y

Y

Q

R

K

Q

G

G

G

V

G

G

V

V

P

P

C

G

L

L

I

I

A

A

V

V

H

H

H

N

N

D

A

H

T

T

G

G

N

K

A

A

L

L

D

E

L

T

G

G

L

L

A

A

Y

Y

A

A

S

K

A

G

I

I

G

G

G

C

G

T

R

R

S

A

G

G

I

V

I

I

E

A

T

T

N

T

F

F

R

K

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

A

C

V

V

L

L

C

C

G

G

T

V

V

T

E

E

L

L

V

I

R

K

A

A

G

G

F

F

E

D

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

Q

P

P

E

E

M

I

A

A

Y

Y

F

F

E
|
E

C

C

L

L

H

H

E
|
E

L

L

K

K

L

L

I

I

V

V

D

D

L

L

M

I

Y

Y

E

E

G

G

I

I

A

G

N

L

M

M

N

R

Y

Y

S

S

I

V

S

S

N

D

T

T

A

A

E

E

Y

Y

G

G

E

D

Y

L

V

T

T

V

G

G

P

P

R

R

I

I

T

N

P

E

E

N

T

T

K

R

A

A

E

E

M

M

K

K

R

K

V

V

L

L

E

A

D

A

I

I

Q

Q

S

D

G

G

K

T

F

F

V

A

R

R

D

E

F

L

M

L

L

L

E

E

N

F

A

Q

V

V

G

G

Q

R

P

P

S

V

F

F

K

S

A

A

T

L

R

R

R

K

S

G

A

Q

E

E

H

H

Q

L

I

I

E

E

E

K

V

V

G

G

A

K

R

E

L

L

R

R

G

A

M

M

P

P

W

W

I

L

binding magnesium ion: D195 (= D191), E199 (= E195), E231 (= E227), E235 (= E231)
binding 1,4-dihydronicotinamide adenine dinucleotide: Y24 (= Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), R47 (= R48), E57 (= E58), L84 (= L80), V85 (≠ A81), P86 (= P82), D87 (= D83), V93 (≠ I89), S111 (≠ A107), H112 (= H108)

6jx2A Crystal structure of ketol-acid reductoisomerase from corynebacterium glutamicum (see paper)
60% identity, 96% coverage: 1:325/339 of query aligns to 1:324/325 of 6jx2A

query
sites
6jx2A

M

I

R

E

V

L

Y

L

Y

Y

D

D

R

A

D

D

A

A

D

D

L

L

A

S

R

L

I

I

L

Q

D

G

R

R

K

K

I

V

A

A

I

I

V

V

G
|
G

Y
|
Y

G
|
G

S

S

Q

Q

G

G

H

H

A

A

H

H

A

S

L

Q

N

N

L

L

R

R

D

D

S

S

G

G

I

V

K

E

N

-

V

V

A

V

V

I

A

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L

L

R
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R

A

E

G

G

S
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S

P
x
K

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x
S

A

A

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E

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K

A

A

E

K

G

E

E

A

G

G

L

F

K

E

V

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M

K

T

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A

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E

A

A

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A

A

D

D

L

V

I

I

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M

I

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L

A
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A

P
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P

D

D

E

T

H

S

Q

Q

A

A

A

E

I
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I

Y

F

K

T

N

N

E

D

I

I

A

E

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P

N

N

I

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R
x
N

D

A

G

G

A
x
D

A

A

L

L

F

F

A

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H

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G

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A

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A

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D
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D

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E

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A

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C

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A

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G

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N

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M

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N

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Y

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N

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T

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A

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E

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Y

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G

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F

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K

D

R

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L

M

I

L

A

E

N

N

V

A

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N

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G

Q

N

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S

E

F

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E

A

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A

R

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S

Y

A

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N

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L

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M

binding magnesium ion: N98 (≠ R99), D101 (≠ A102), D190 (= D191), E194 (= E195)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G23 (= G23), Y24 (= Y24), G25 (= G25), R47 (= R48), S50 (= S51), K51 (≠ P52), S52 (≠ T53), A80 (= A81), P81 (= P82), I88 (= I89)

D0WGK0 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Slackia exigua (strain ATCC 700122 / DSM 15923 / CIP 105133 / JCM 11022 / KCTC 5966 / S-7) (see paper)
54% identity, 97% coverage: 1:330/339 of query aligns to 12:340/342 of D0WGK0

query
sites
D0WGK0

M

V

R

T

V

I

Y

L

Y

Y

D

E

R

Q

D

D

A

V

D

D

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I

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K

I

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A

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I

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Y
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Y

G
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G

S
|
S

Q
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Q

G

G

H

H

A

A

H

H

A

A

L

L

N

N

L

L

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M

D

D

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S

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N

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A

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R

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S

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x
S

A

W

K

K

K

T

A

A

E

E

G

E

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A

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G

L

L

K

K

V

V

M

T

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D

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M

A

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E

A

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M

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D

E
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E

H
x
I

Q
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Q

A

P

A

K

I

V

Y

Y

K

Q

N

E

E

H

I

I

A

A

L

A

N

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I

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D

A

G

G

A

N

A

T

L

L

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A

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F

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P

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K

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K

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E

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M

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R

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G

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YGSQ 35:38 (= YGSQ 24:27) binding NADP(+)
R58 (= R48) binding NADP(+)
S61 (= S51) binding NADP(+); mutation to D: Inversion of cofactor specificity from NADPH to NADH. Strong decrease of the affinity for NADPH and 2.5-fold decrease of the affinity for NADH. 8-fold decrease of the catalytic efficiency for NADPH and 2-fold increase of the catalytic efficiency for NADH; when associated with D-63.
S63 (≠ T53) binding NADP(+); mutation to D: Inversion of cofactor specificity from NADPH to NADH. Strong decrease of the affinity for NADPH and 2.5-fold decrease of the affinity for NADH. 8-fold decrease of the catalytic efficiency for NADPH and 2-fold increase of the catalytic efficiency for NADH; when associated with D-61.
DEIQ 93:96 (≠ DEHQ 83:86) binding NADP(+)
G144 (= G134) binding NADP(+)
D201 (= D191) binding Mg(2+)
E205 (= E195) binding Mg(2+)
S262 (= S252) binding substrate

4kqxB Mutant slackia exigua kari ddv in complex with NAD and an inhibitor (see paper)
53% identity, 98% coverage: 1:333/339 of query aligns to 3:334/335 of 4kqxB

query
sites
4kqxB

M

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Y

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H

A

A

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L

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-

N

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A

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L

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K

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x
E

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x
V

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x
N

A

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A

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M

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Y

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I
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I

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A

E
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G

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E

Y

Y

V

Y

T

A

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G

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P

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P

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E

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A

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M

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K

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D

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S

F

F

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F

F

M

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D

E

D

N

C

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N

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R

F

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K

L

A

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x
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L
|
L

active site: K132 (= K131), D192 (= D191), E196 (= E195)
binding n-hydroxy-n-isopropyloxamic acid: P134 (= P133), D192 (= D191), E196 (= E195), E232 (= E231), I252 (= I251), S253 (= S252), A256 (= A255)
binding histidine: D333 (≠ K332), L334 (= L333)
binding magnesium ion: G27 (= G25), Q29 (= Q27), G30 (= G28), A72 (= A71), E73 (≠ A72), A75 (= A74), L81 (= L80), N103 (≠ A102), D192 (= D191), E196 (= E195), N249 (= N248), S251 (= S250), I252 (= I251), I252 (= I251), S253 (= S252), N254 (= N253), E257 (= E256)
binding nicotinamide-adenine-dinucleotide: Y26 (= Y24), G27 (= G25), S28 (= S26), Q29 (= Q27), R49 (= R48), L81 (= L80), V82 (≠ A81), P83 (= P82), D84 (= D83), V90 (≠ I89), H109 (= H108), P134 (= P133), S251 (= S250), I252 (= I251), S253 (= S252)

Sites not aligning to the query:

binding histidine: 335

4kqwA The structure of the slackia exigua kari in complex with NADP (see paper)
54% identity, 96% coverage: 3:328/339 of query aligns to 2:326/326 of 4kqwA

query
sites
4kqwA

V

I

Y

L

Y

Y

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D

D

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D

D

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S
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Q
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Q

G

G

H

H

A

A

H

H

A

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N

N

L

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M

D

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S

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G

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D

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R

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x
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A

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x
I

Q
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A

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L

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D

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A

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A

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Y

F

F

E

E

C

C

L

Y

H

H

E

E

L

M

K

K

L

M

I

I

V

V

D

D

L

L

M

M

Y

Y

E

E

G

S

G

G

I

I

A

H

N

F

M

M

N

N

Y

Y

S

S

I
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I

S
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S

N

N

T

T

A

A

E

E

Y

Y

G

G

E

E

Y

Y

V

Y

T

A

G

G

P

P

R

K

I

V

I

I

T

N

P

E

E

Q

T

S

K

R

A

E

E

A

M

M

K

K

R

E

V

I

L

L

E

K

D

R

I

I

Q

Q

S

D

G

G

K

S

F

F

V

A

R

Q

D

E

F

F

M

V

L

D

E

D

N

C

A

N

V

N

G

G

Q

H

P

K

S

R

F

L

K

L

A

E

T

Q

R

R

R

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R

A

S

I

A

N

E

T

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H

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T

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G

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M

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S

W

W

I

I

active site: K129 (= K131), D189 (= D191), E193 (= E195)
binding magnesium ion: D189 (= D191), E193 (= E195)
binding nadp nicotinamide-adenine-dinucleotide phosphate: Y23 (= Y24), G24 (= G25), S25 (= S26), Q26 (= Q27), R46 (= R48), S49 (= S51), S51 (≠ T53), L78 (= L80), V79 (≠ A81), P80 (= P82), D81 (= D83), I83 (≠ H85), Q84 (= Q86), H106 (= H108), P131 (= P133), I249 (= I251), S250 (= S252)

6bulB Crystal structure of staphylococcus aureus ketol-acid reductoisomerase with hydroxyoxamate inhibitor 2
56% identity, 96% coverage: 3:328/339 of query aligns to 3:326/327 of 6bulB

query
sites
6bulB

V

V

Y

Y

D

D

R

Q

D

D

A

V

D

K

L

T

A

D

R

A

I

L

L

Q

D

G

R

K

K

K

I

I

A

A

I

V

V

V

G

G

Y
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Y

G
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G

S
|
S

Q
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Q

G

G

H

H

A

A

H

H

A

A

L

Q

N

N

L

L

R

K

D

D

S

N

G

G

I

Y

K

-

N

D

V

V

A

V

V

I

A

G

L
x
I

R
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R

A

P

G

G

S

R

P

-

T
x
S

A

F

K

D

K

K

A

A

E

K

G

E

E

D

G

G

L

F

K

D

V

V

M

F

T

P

V

V

A

A

E

E

A

A

A

V

A

K

W

Q

A

A

D

D

L

V

I

I

M

M

I

V

L
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L

A
x
L

P
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P

D
|
D

E

E

H

I

Q

Q

A

G

A

D

I

V

Y

Y

K

K

N

N

E

E

I

I

A

E

L

P

N

N

I

L

R

E

D

K

G

H

A

N

A

A

L

L

L

A

F

F

A

A

H
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H

G

G

L

F

N

N

V

I

H

H

F

F

G

G

L

V

I

I

E

Q

P

P

K

P

D

A

T

D

I

V

D

D

V

V

L

F

M

L

V

V

A

A

P

P

K
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K

G

G

P
|
P

G

G

H

H

T

L

V

V

R

R

G

R

E

T

Y

F

Q

V

K

E

G

G

G

S

G

A

V

V

P

P

C

S

L

L

I

F

A

G

V

I

H

Q

N

D

A

A

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G

G

N

Q

A

A

L

R

D

N

L

I

G

A

L

L

A

S

Y

Y

A

A

S

K

A

G

I

I

G

G

G

A

G

T

R

R

S

A

G

G

I

V

I

I

E

E

T

T

N

T

F

F

R

K

E

E

C

T

E

E

T

T

D
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D

L

L

F

F

G

G

E
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E

Q

Q

A

A

V

V

L

L

C

C

G

G

T

V

V

S

E

K

L

L

V

I

R

Q

A

S

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

Q

P

P

E

E

M

L

A

A

Y

Y

F

F

E

E

C

V

L

L

H

H

E
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E

L

M

K

K

L

L

I
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I

V

V

D

D

L

L

M

M

Y

Y

E

E

G

G

I

M

A

E

N

N

M

V

N

R

Y

Y

S
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S

I
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I

S
|
S

N

N

T

T

A
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A

E

E

Y

F

G

G

E

D

Y

Y

V

V

T

S

G

G

P

P

R

R

I

V

I

I

T

T

P

P

E

D

T

V

K

K

A

E

E

N

M

M

K

K

R

A

V

V

L

L

E

T

D

D

I

I

Q

Q

S

N

G

G

K

N

F

F

V

S

R

N

D

R

F

F

M

I

L

E

E

D

N

N

A

K

V

N

G

G

Q

F

P

K

S

E

F

F

K

Y

A

K

T

L

R

R

R

E

S

Q

A

H

E

G

H

H

Q

Q

I

I

E

E

E

K

V

V

G

G

A

R

R

E

L

L

R

R

G

E

M

M

P

P

W

F

I

I

active site: K129 (= K131), D189 (= D191), E193 (= E195)
binding {hydroxy[(1S)-1-phenylethyl]amino}(oxo)acetic acid: P131 (= P133), D189 (= D191), E193 (= E195), E229 (= E231), I233 (= I235), I249 (= I251), S250 (= S252), A253 (= A255)
binding oxo{[(1S)-1-phenylethyl]amino}acetic acid: P131 (= P133), D189 (= D191), E193 (= E195), E229 (= E231), I233 (= I235), I249 (= I251), S250 (= S252), A253 (= A255)
binding magnesium ion: D189 (= D191), E193 (= E195)
binding nadp nicotinamide-adenine-dinucleotide phosphate: Y24 (= Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), I46 (≠ L47), R47 (= R48), S51 (≠ T53), L78 (= L80), L79 (≠ A81), P80 (= P82), D81 (= D83), H106 (= H108), P131 (= P133), S248 (= S250), I249 (= I251), S250 (= S252)

Query Sequence

>CCNA_02204 FitnessBrowser__Caulo:CCNA_02204
MRVYYDRDADLARILDRKIAIVGYGSQGHAHALNLRDSGIKNVAVALRAGSPTAKKAEGE
GLKVMTVAEAAAWADLIMILAPDEHQAAIYKNEIALNIRDGAALLFAHGLNVHFGLIEPK
DTIDVLMVAPKGPGHTVRGEYQKGGGVPCLIAVHHNATGNALDLGLAYASAIGGGRSGII
ETNFREECETDLFGEQAVLCGGTVELVRAGFETLVEAGYAPEMAYFECLHELKLIVDLMY
EGGIANMNYSISNTAEYGEYVTGPRIITPETKAEMKRVLEDIQSGKFVRDFMLENAVGQP
SFKATRRRSAEHQIEEVGARLRGMMPWIAKNKLVDQAKN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory