SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CCNA_02221 FitnessBrowser__Caulo:CCNA_02221 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
64% identity, 98% coverage: 8:889/899 of query aligns to 355:1225/1227 of P13009

query
sites
P13009

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E694 (= E350) binding
GDVHD 756:760 (= GDVHD 414:418) binding
D757 (= D415) mutation to E: Decreases activity by about 70%.; mutation to N: Decreases activity by about 45%.
H759 (= H417) binding axial binding residue; mutation to G: Loss of catalytic activity.
S804 (= S462) binding
T808 (= T466) binding
S810 (= S468) mutation to A: Decreases activity by about 40%.
A860 (= A519) binding
D946 (= D605) binding
R1134 (= R798) binding
YY 1189:1190 (≠ YF 853:854) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
247 binding
310 binding ; mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
311 binding ; mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.

Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
56% identity, 98% coverage: 9:889/899 of query aligns to 371:1263/1265 of Q99707

query
sites
Q99707

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V

K

K

G

G

D

D

V

V

H

H

D

D

I

I

G

G

K

K

N

N

I

I

V

V

G

G

V

V

L

L

Q

G

C

C

N

N

Y

F

E

R

V

V

V

I

D

D

L

L

G

G

V

V

M

M

V

T

P

P

A

C

D

D

R

K

I

I

L

L

D

K

E

A

A

A

K

L

K

D

H

H

K

K

V

A

D

D

M

I

I

I

G

G

L

L

S

S

G

G

L

L

I

I

T

T

P

P

S

S

L

L

D

D

E

E

M

M

V

I

F

F

V

V

A

A

A

K

E

E

M

M

E

E

R

R

Q

L

G

A

F

I

D

R

I

I

P

P

L

L

I

I

G

G

A

A

T

T

S

S

R

K

T

T

H

H

T

T

A

A

V

V

K

K

I

I

E

A

P

P

A

R

Y

Y

R

-

R

S

G

A

P

P

T

V

T

I

Y

H

V

V

V

L

D

D

A

A

S

S

R

K

A

S

V

V

G

V

V

V

V

C

S

S

G

Q

L

L

S

D

E

E

G

N

E

L

R

K

D

D

R

E

I

Y

I

F

A

E

E

E

T

I

R

M

A

E

E

E

Y

Y

V

E

K

D

V

I

R

R

E

Q

Q
x
D

Y

H

A

Y

R

E

G

S

Q

L

T

K

T

E

K

R

A

R

R

Y

A

L

S

P

I

L

Q

S

E

Q

A

A

R

R

K

K

R

S

A

G

F

F

A

Q

I

M

D

D

W

W

K

L

G

S

Y

E

A

P

P

H

P

P

-

V

K

K

P

P

A

T

F

F

I

I

G

G

T

T

R

Q

V

V

F

F

E

E

P
x
D

-

Y

S

D

L

L

A

Q

E

K

L

L

V

V

P

D

F

Y

I

I

D

D

W

W

S

K

P

P

F

F

A

D

S

V

W

W

E

Q

L

L

I

R

G

G

R

K

-

Y

-

P

-

N

-

R

-

G

F

F

P

P

Q

K

I

I

L

F

E

N

D

D

D

K

V

T

V

V

G

G

Q

G

A

E

A

A

T

R

D

K

L

V

Y

Y

R

D

D

D

A

A

R

H

A

N

M

M

L

L

D

N

K

T

V

L

V

I

E

S

E

Q

K

K

W

K

F

L

G

R

A

A

K

R

G

G

V

V

I

V

G

G

F

F

W

W

P

P

A

A

Q

Q

A

S

Q

I

G

Q

D

D

I

I

V

H

L

L

Y

Y

T

A

D

E

E

A

T

A

-

V

-

P

R

Q

V

A

A

A

E

E

-

P

F

I

S

A

R

T

L

F

H

Y

T

G

L

L

R

R

Q

Q

M

A

D

E

K
|
K

G

-

A

-

D

-

K

D

S

S

G

A

E

S

A

T

K

E

A

P

N

Y

V

Y

A

C

L

L

S

S

D

D

F

F

V

I

A

A

P

P

I

L

G

H

Q

S

G

G

A

I

-

R

D

D

Y

Y

V

L

G

G

G

L

F

F

A

A

V

V

T

-

A

A

G

C

H

F

G

G

E

V

D

E

E

E

I

L

V

S

A

K

K

A

F

Y

K

E

A

D

G

G

D

D

Y

Y

N

S

A

S

I

I

M

M

A

V

S

K

A

A

L

L

A

G

D

D

R

R

L

L

A

A

E

E

A

A

F

F

A

A

E

E

W

E

L

L

H

H

Y

E

K

R

A

V

R

R

V

R

E

E

L

L

W

W

G

A

Y

Y

A

C

A

G

D

S

E

E

D

Q

A

L

D

D

V

V

E

A

R

D

L

L

I

R

A

R

E

L

K

R

Y

Y

Q

K

G

G

I

I

R

R

P

P

A

A

P

P

G

G

Y

Y

P

P

A

S

Q

Q

P

P

D

D

H

H

T

T

E

E

K

K

G

L

T

T

L

M

F

W

K

R

L

L

L

A

D

D

A

I

E

E

A

Q

A

S

T

T

G

G

L

I

Q

R

L

L

T

T

E

E

S

S

Y

L

A

A

M

M

T

A

P

P

G

A

A

S

A

A

V

V

S

S

G

G

L

L

F

Y

F

F

S

S

H

N

R

L

Q

K

A

S

H

K

Y

Y

F

F

G

A

V

V

G

G

K

K

I

I

D

S

A

K

D

D

Q

Q

V

V

E

E

D

D

Y

Y

A

A

R

L

R

R

K

K

G

N

W

I

D

S

M

V

E

A

T

E

A

V

E

E

R

K

W

W

L

L

S

G

P

P

I

I

L

L

N

G

Y

Y

D

D

GSR 382:384 (≠ GSA 20:22) binding
D449 (= D87) binding
N470 (= N108) binding
D537 (= D175) binding
N579 (= N217) binding
R585 (= R223) binding
R591 (= R229) binding
D919 (≠ Q552) to G: in dbSNP:rs1805087
D963 (≠ P595) mutation to E: Decreases binding to MTRR; when associated with N-1071.
K1071 (= K694) mutation to N: Decreases binding to MTRR; when associated with E-963.

Sites not aligning to the query:

61 natural variant: R -> K
255 C → Y: in dbSNP:rs1140598

3bulA E. Coli i690c/g743c meth c-terminal fragment (649-1227) (see paper)
61% identity, 65% coverage: 309:889/899 of query aligns to 3:575/577 of 3bulA

query
sites
3bulA

E

E

W

W

R

R

K

S

G

W

T

E

V

V

N

N

E

K

R

R

L

L

T

E

H

Y

A

S

L

L

V

V

H

K

G

G

I

I

T

T

E

E

F

F

I

I

E

E

Q

Q

D

D

T

T

E

E

A

A

R

R

L

Q

A

Q

A

A

E

T

R

R

P

P

L

C

H

E

V

V

I

I

E

E

G

G

P

P

L

L

M

M

D

D

G

G

M

M

N

N

V

V

G

G

D

D

L

L

F

F

G

G

A

E

G

G

K

K

M

M

F

F

L

L

P

P

Q

Q

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

Q

Q

A

A

V

V

A

A

W

Y

L

L

M

E

P

P

F

F

M

I

E

E

A

A

E

S

K

K

E

E

G

-

Q

-

E

Q

R

C

K

K

A

T

A

N

G

G

K

K

V

M

L

V

M

I

A

A

T

T

V

V

K

K

G

G

D
|
D

V
|
V

H
|
H

D

D

I

I

G
|
G

K

K

N

N

I

I

V
|
V

G

G

V

V

L

L

Q

Q

C

C

N

N

Y

Y

E

E

V

I

V

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

A

A

D

E

R

K

I

I

L

L

D

R

E

T

A

A

K

K

K

E

H

V

K

N

V

A

D

D

M

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

V

V

F

N

V

V

A

A

A

K

E

E

M

M

E

E

R

R

Q

Q

G

G

F

F

D

T

I

I

P

P

L

L

L
|
L

I

I

G
|
G

A
|
A

T
|
T

T

T

S

S

R

K

T

A

H

H

T

T

A

A

V

V

K

K

I

I

E

E

P

Q

A

N

Y

Y

R

S

R

-

G

G

P

P

T

T

T

V

Y

Y

V
|
V

V
x
Q

D
x
N

A
|
A

S

S

R

R

A
x
T

V

V

G

G

V

V

S

A

G

A

L

L

S

S

E

D

G

T

E

Q

R

R

D

D

R

D

I

F

I

V

A

A

E

R

T

T

R

R

A

K

E

E

Y

Y

V

E

K

T

V

V

R

R

E

I

Q

Q

Y

H

A

G

R

R

G

K

Q

K

T

P

T

R

K

T

A

P

R

P

A

V

S

T

I

L

Q

E

E

A

A

A

R

R

K

D

R

N

A

D

F

F

A

A

I

F

D

D

W

W

K

Q

G

A

Y

Y

A

T

P

P

K

V

P

A

A

H

F

R

I

L

G

G

T

V

R

Q

V

E

F

V

E

E

P

A

S

S

L

I

A

E

E

T

L

L

V

R

P

N

F

Y

I

I

D

D

W

W

S

T

P

P

F

F

A
x
M

S
x
T

W

W

E

S

L

L

I

A

G

G

R

K

F

Y

P

P

Q

R

I

I

L

L

E

E

D

D

D

E

V

V

G

G

Q

V

A

E

A

A

T

Q

D

R

L

L

Y

F

R

K

D

D

A

A

R

N

A

D

M

M

L

L

D

D

K

K

V

L

V

S

E

A

E

E

K

K

W

T

F

L

G

N

A

P

K

R

G

G

V

V

I

V

G

G

F

L

W

F

P

P

A

A

Q

N

A

R

Q

V

G

G

D

D

I

I

V

E

L

I

Y

Y

T

R

D

D

E

E

T

T

R

R

V

T

A

H

E

V

F

I

S

N

R

V

L

S

H

H

T

H

L

L

R

R

Q

Q

M

T

D

E

K

K

G

-

A

-

D

-

K

-

S

-

G

-

E

T

A

G

K

F

A

A

N

N

V

Y

A

C

L

L

S

A

D

D

F

F

V

V

A

A

P

P

I

K

G

L

Q

S

G

G

-

K

A

A

D

D

Y

Y

V

I

G

G

G

A

F

F

A

A

V

V

T

T

A

G

G

G

H

L

G

E

E

E

D

D

E

A

I

L

V

A

A

D

K

A

F

F

K

E

A

A

A

Q

G

H

D

D

Y

Y

N

N

A

K

I

I

M

M

A

V

S

K

A

A

L

L

A

A

D
|
D

R

R

L

L

A

A

E

E

A

A

F

F

A

A

E

E

W

Y

L

L

H

H

Y

E

K

R

A

V

R

R

V

K

E

V

L

Y

W

W

G

G

Y

Y

A

A

A

P

D

N

E

E

D

N

A

L

D

S

V

N

E

E

R

E

L

L

I

I

A

R

E

E

K

N

Y

Y

Q

Q

G

G

I

I

R

R

P

P

A
|
A

P
|
P

G
|
G

Y
|
Y

P
|
P

A

A

Q

C

P

P

D

E

H
|
H

T

T

E

E

K
|
K

G

A

T

T

L

I

F

W

K

E

L

L

D

E

A

V

E

E

A

K

A

H

T

T

G

G

L

M

Q

K

L

L

T

T

E

E

S

S

Y

F

A
|
A

M
|
M

T

W

P

P

G
|
G

A

A

A
x
S

V
|
V

S
|
S

G

G

L

W

F

Y

F

F

S

S

H

H

R

P

Q

D

A

S

H

K

Y

Y

F

Y

G

A

V

V

G

A

K

Q

I

I

D

Q

A

R

D

D

Q

Q

V

V

E

E

D

D

Y

Y

A

A

R

R

K

K

G

G

W

M

D

S

M

V

E

T

T

E

A

V

E

E

R

R

W

W

L

L

S

A

P

P

I

N

L

L

N

G

Y

Y

D

D

active site: D107 (= D415), H109 (= H417), S160 (= S468)
binding cobalamin: V108 (= V416), H109 (= H417), G112 (= G420), V116 (= V424), G152 (= G460), L153 (= L461), S154 (= S462), L156 (= L464), I157 (= I465), T158 (= T466), L181 (= L489), G183 (= G491), G184 (= G492), A185 (= A493), T186 (= T494), V207 (= V516), Q208 (≠ V517), N209 (≠ D518), A210 (= A519), T213 (≠ A522), M302 (≠ A611), T303 (≠ S612), D443 (= D757), A486 (= A800), P487 (= P801), G488 (= G802), Y489 (= Y803), P490 (= P804), H495 (= H809), K498 (= K812), A520 (= A834), M521 (= M835), G524 (= G838), S526 (≠ A840), V527 (= V841), S528 (= S842)

3ivaA Structure of the b12-dependent methionine synthase (meth) c-teminal half with adohcy bound (see paper)
61% identity, 65% coverage: 309:889/899 of query aligns to 3:575/576 of 3ivaA

query
sites
3ivaA

E

E

W

W

R

R

K

S

G

W

T

E

V

V

N

N

E

K

R

R

L

L

T

E

H

Y

A

S

L

L

V

V

H

K

G

G

I

I

T

T

E

E

F

F

I

I

E

E

Q

Q

D

D

T

T

E

E

A

A

R

R

L

Q

A

Q

A

A

E

T

R

R

P

P

L

C

H

E

V

V

I

I

E

E

G

G

P

P

L

L

M

M

D

D

G

G

M

M

N

N

V

V

G

G

D

D

L

L

F

F

G

G

A

E

G

G

K

K

M

M

F

F

L

L

P

P

Q

Q

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

Q

Q

A

A

V

V

A

A

W

Y

L

L

M

E

P

P

F

F

M

I

E

E

A

A

E

S

K

K

E

E

G

-

Q

-

E

Q

R

C

K

K

A

T

A

N

G

G

K

K

V

M

L

V

M
x
I

A

A

T

T

V

V

K

K

G

G

D
|
D

V
|
V

H
|
H

D

D

I

I

G
|
G

K
|
K

N

N

I
|
I

V
|
V

G

G

V

V

L

L

Q

Q

C

C

N

N

Y

Y

E

E

V

I

V

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

A

A

D

E

R

K

I

I

L

L

D

R

E

T

A

A

K

K

K

E

H

V

K

N

V

A

D

D

M

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

V

V

F

N

V

V

A

A

A

K

E

E

M

M

E

E

R

R

Q

Q

G

G

F

F

D

T

I

I

P

P

L

L

L
|
L

I

I

G
|
G

A
|
A

T

T

S

S

R

K

T

A

H

H

T

T

A

A

V

V

K

K

I

I

E

E

P

Q

A

N

Y

Y

R

S

R

-

G

G

P

P

T

T

T

V

Y

Y

V
|
V

V
x
Q

D
x
N

A
|
A

S

S

R

R

A
x
T

V

V

G

G

V

V

S

A

G

A

L

L

S

S

E

D

G

T

E

Q

R

R

D

D

R

D

I

F

I

V

A

A

E

R

T

T

R

R

A

K

E

E

Y

Y

V

E

K

T

V

V

R

R

E

I

Q

Q

Y

H

A

G

R

R

G

K

Q

K

T

P

T

R

K

T

A

P

R

P

A

V

S

T

I

L

Q

E

E

A

A

A

R

R

K

D

R

N

A

D

F

F

A

A

I

F

D

D

W

W

K

Q

G

A

Y

Y

A

T

P

P

K

V

P

A

A

H

F

R

I

L

G

G

T

V

R

Q

V

E

F

V

E

E

P

A

S

S

L

I

A

E

E

T

L

L

V

R

P

N

F

Y

I

I

D
|
D

W

W

S

T

P
|
P

F

F

A

M

S
x
T

W

W

E

S

L

L

I

A

G

G

R

K

F

Y

P

P

Q

R

I

I

L

L

E

E

D

D

D

E

V

V

G

G

Q

V

A

E

A

A

T

Q

D

R

L

L

Y

F

R

K

D

D

A

A

R

N

A

D

M

M

L

L

D

D

K

K

V

L

V

S

E

A

E

E

K

K

W

T

F

L

G

N

A

P

K

R

G

G

V

V

I

V

G

G

F

L

W

F

P

P

A

A

Q

N

A

R

Q

V

G

G

D

D

I

I

V

E

L

I

Y

Y

T

R

D

D

E

E

T

T

R

R

V

T

A

H

E

V

F

I

S

N

R

V

L

S

H

H

T

H

L

L

R

R

Q

Q

M

T

D

E

K

K

G

-

A

-

D

-

K

-

S

-

G

-

E

T

A

G

K

F

A

A

N

N

V

Y

A

C

L

L

S

A

D

D

F

F

V

V

A

A

P

P

I

K

G

L

Q

S

G

G

-

K

A

A

D

D

Y

Y

V

I

G

G

G

A

F

F

A

A

V

V

T

T

A

G

G

G

H

L

G

E

E

E

D

D

E

A

I

L

V

A

A

D

K

A

F

F

K

E

A

A

A

Q

G

H

D

D

Y

Y

N

N

A

K

I

I

M

M

A

V

S

K

A

A

L

L

A

A

D
|
D

R
|
R

L

L

A

A

E
|
E

A

A

F

F

A

A

E

E

W

Y

L

L

H

H

Y

E

K

R

A

V

R

R

V

K

E

V

L

Y

W

W

G

G

Y

Y

A

A

A

P

D

N

E

E

D

N

A

L

D

S

V

N

E

E

R

E

L

L

I

I

A

R

E
|
E

K

N

Y

Y

Q

Q

G

G

I

I

R
|
R

P
|
P

A
|
A

P
|
P

G
|
G

Y
|
Y

P
|
P

A
|
A

Q

C

P

P

D

E

H
|
H

T

T

E

E

K
|
K

G

A

T

T

L

I

F

W

K

E

L

L

D

E

A

V

E

E

A

K

A

H

T

T

G

G

L

M

Q

K

L

L

T

T

E

E

S

S

Y

F

A
|
A

M
|
M

T

W

P

P

G
|
G

A

A

A
x
S

V
|
V

S
|
S

G

G

L

W

F

Y

F

F

S

S

H

H

R

P

Q

D

A

S

H

K

Y
|
Y

F
x
Y

G

A

V

V

G

A

K

Q

I

I

D

Q

A

R

D

D

Q

Q

V

V

E

E

D

D

Y

Y

A

A

R

R

K

K

G

G

W

M

D

S

M

V

E

T

T

E

A

V

E

E

R

R

W

W

L

L

S

A

P

P

I

N

L

L

N

G

Y

Y

D

D

active site: D107 (= D415), H109 (= H417), S160 (= S468)
binding cobalamin: I101 (≠ M409), V108 (= V416), H109 (= H417), G112 (= G420), K113 (= K421), I115 (= I423), V116 (= V424), G152 (= G460), L153 (= L461), S154 (= S462), L156 (= L464), I157 (= I465), T158 (= T466), L181 (= L489), G183 (= G491), G184 (= G492), A185 (= A493), V207 (= V516), Q208 (≠ V517), N209 (≠ D518), A210 (= A519), T213 (≠ A522), T303 (≠ S612), D443 (= D757), R444 (= R758), A486 (= A800), P487 (= P801), G488 (= G802), Y489 (= Y803), P490 (= P804), H495 (= H809), K498 (= K812), A520 (= A834), M521 (= M835), G524 (= G838), S526 (≠ A840), V527 (= V841), S528 (= S842)
binding s-adenosyl-l-homocysteine: D296 (= D605), P299 (= P608), R444 (= R758), E447 (= E761), E478 (= E792), R484 (= R798), P485 (= P799), A486 (= A800), Y489 (= Y803), P490 (= P804), A491 (= A805), Y539 (= Y853), Y540 (≠ F854)

3k13C Structure of the pterin-binding domain metr of 5- methyltetrahydrofolate-homocysteine methyltransferase from bacteroides thetaiotaomicron
69% identity, 32% coverage: 9:297/899 of query aligns to 4:286/287 of 3k13C

query
sites
3k13C

F

F

V

V

N

N

I

I

G

G

E
|
E

R

R

T

C

N
|
N

V

V

T

A

G
|
G

S
|
S

A
x
R

K

K

F

F

K

L

K

R

L

L

I

V

V

N

E

E

G

K

N

K

Y

Y

P

D

E

E

A

A

L

L

S

S

V

I

A

A

R

R

Q

Q

V

V

E

E

A

D

G

G

A

A

Q

L

V

V

I

I

D

D

V

V

N

N

M

M

D

D

E

D

G

G

L

L

D

D

S

A

Q

R

Q

T

A

E

M

M

V

T

T

T

F

F

L

L

N

N

L

L

M

I

A

M

A

S

E

E

P

P

D

E

I

I

A

A

R

R

V

V

P

P

V

V

M

M

I

I

D
|
D

S

S

K

K

W

W

E

E

V

V

I

I

E

E

A

A

G

G

L

L

K

K

C

C

V

L

Q

Q

G

G

K

K

A

S

I

I

V

V

N
|
N

S

S

I

I

S

S

L

L

K

K

E

E

G

G

E

E

K

V

F

F

L

L

E

E

Q

H

A

A

T

R

L

I

C

I

L

K

R

Q

Y

Y

G

G

A

A

V

T

V

V

M
|
M

A

A

F

F

D

D

E

E

V

K

G

G

Q

Q

A

A

D

D

T

T

E

A

K

A

R

R

K

K

V

I

E

E

I

V

C

C

T

E

R

R

A

A

Y

Y

N

R

T

L

L

L

V

V

D

D

K

K

V

V

G

G

F

F

P

N

P

P

E

H

D

D

I

I

F

F

D
|
D

P

P

N

N

I

V

F

L

A

A

V

V

A

A

T

T

G

G

I

I

E

E

H

H

D

N

N

N

Y

Y

A

A

V

V

D

D

F

F

I

I

E

E

A

A

T

T

R

G

R

W

I

I

K

R

Q

K

M

N

L

L

P

P

Y

G

A

A

R

H

V

V

S

S

G

G

G
|
G

V

V

S
|
S

N
|
N

V

L

S

S

F

F

S

S

F

F

R
|
R

G

G

N

N

E

N

P

Y

V

I

R
|
R

R

E

A

A

I

M

H

H

S

A

V

V

F

F

L

L

Y

Y

H

H

A

A

I

I

N

Q

A

Q

G

G

M

M

D

D

M

M

G

G

I
|
I

V

V

N

N

A

P

G

G

D

S

L

V

P

-

V

L

Y

Y

D

S

D

D

I

I

D

P

P

A

A

D

L

T

R

L

E

E

A

K

V

I

E

E

D

D

V

V

I

V

L

L

N

N

R

R

P

R

Q

P

R

-

D

-

P

-

V

-

M

-

T

D

N

A

T

A

E

E

R

R

L

L

V

I

E

E

M

L

A

A

P

E

R

A

Y

L

K

K

binding n-[4-({[(6s)-2-amino-4-hydroxy-5-methyl-5,6,7,8-tetrahydropteridin-6-yl]methyl}amino)benzoyl]-l-glutamic acid: E9 (= E14), N12 (= N17), G15 (= G20), S16 (= S21), R17 (≠ A22), D82 (= D87), N103 (= N108), M131 (= M136), D170 (= D175), G209 (= G214), S211 (= S216), N212 (= N217), R218 (= R223), R224 (= R229), I244 (= I249)

4cczA Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
64% identity, 32% coverage: 9:293/899 of query aligns to 331:610/611 of 4cczA

query
sites
4cczA

F

F

V

V

N

N

I

I

G

G

E
|
E

R

R

T

C

N
|
N

V

V

T

A

G
|
G

S
|
S

A
x
R

K

K

F

F

K

A

K

K

L

L

I

I

V

M

E

A

G

G

N

N

Y

Y

P

E

E

E

A

A

L

L

S

C

V

V

A

A

R

K

Q

V

Q

Q

V

V

E

E

A

M

G

G

A

A

Q

Q

V

V

I

L

D

D

V

V

N

N

M

M

D

D

E

D

G

G

L

M

L

L

D

D

S

G

Q

P

Q

S

A

A

M

M

V

T

T

R

F

F

L

C

N

N

L

L

M

I

A

A

A

S

E

E

P

P

D

D

I

I

A

A

R

K

V

V

P

P

V

L

M

C

I

I

D
|
D

S

S

K

N

W

F

E

A

V

V

I

I

E

E

A

A

G

G

L

L

K

K

C

C

V

C

Q

Q

G

G

K

K

A

C

I

I

V

V

N
|
N

S

S

I

I

S

S

L

L

K

K

E

E

G

G

E

E

E

D

K

D

F

F

L

L

E

E

Q

K

A

A

T

R

L

K

C

I

L

K

R

K

Y

Y

G

G

A

A

V

M

V

V

M
|
M

A

A

F

F

D

D

E

E

V

E

G

G

Q

Q

A

A

D

T

T

E

E

T

K

D

R

T

K

K

V

I

E

R

I

V

C

C

T

T

R

R

A

A

Y

Y

N

H

T

L

L

L

V

V

D

K

K

K

V

L

G

G

F

F

P

N

P

P

E

N

D

D

I

I

F

F

D
|
D

P

P

N

N

I

I

F

L

A

T

V

I

A

G

T

T

G

G

I

M

E

E

H

H

D

N

N

L

Y

Y

A

A

V

I

D

N

F

F

I

I

E

H

A

A

T

T

R

K

R

V

I

I

K

K

Q

E

M

T

L

L

P

P

Y

G

A

A

R

R

V

I

S

S

G

G

G
|
G

V

L

S
|
S

N
|
N

V

L

S

S

F
|
F

S

S

F

F

R
|
R

G

G

N

M

E

E

P

A

V

I

R
|
R

R

E

A

A

I

M

H

H

S

G

V

V

F

F

L

L

Y

Y

H

H

A

A

I

I

N

K

A

S

G

G

M

M

D

D

M

M

G

G

I
|
I

V

V

N

N

A

A

G

G

D

N

L

L

P

P

V

V

Y

Y

D

D

I

I

D

H

P

K

A

E

L

L

R

L

E

Q

A

L

V

C

E

E

D

D

V

L

I

I

L

W

N

N

R

-

P

-

Q

-

R

K

D

D

P

P

V

-

M

-

T

E

N

A

T

T

E

E

R

K

L

L

V

L

E

R

M

Y

A

A

binding (6s)-5,6,7,8-tetrahydrofolate: E336 (= E14), N339 (= N17), G342 (= G20), S343 (= S21), R344 (≠ A22), D409 (= D87), N430 (= N108), M458 (= M136), D497 (= D175), G536 (= G214), S538 (= S216), N539 (= N217), F542 (= F220), R545 (= R223), R551 (= R229), I571 (= I249)

1bmtA How a protein binds b12: a 3.O angstrom x-ray structure of the b12- binding domains of methionine synthase (see paper)
74% identity, 27% coverage: 309:555/899 of query aligns to 3:246/246 of 1bmtA

query
sites
1bmtA

E

E

W

W

R

R

K

S

G

W

T

E

V

V

N

N

E

K

R

R

L

L

T

E

H

Y

A

S

L

L

V

V

H

K

G

G

I

I

T

T

E

E

F

F

I

I

E

E

Q

Q

D

D

T

T

E

E

A

A

R

R

L

Q

A

Q

A

A

E

T

R

R

P

P

L

I

H

E

V

V

I

I

E
|
E

G

G

P

P

L

L

M
|
M

D

D

G

G

M
|
M

N

N

V

V

G
|
G

D

D

L

L

F
|
F

G

G

A

E

G

G

K

K

M

M

F

F

L
|
L

P

P

Q

Q

V
|
V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

Q

Q

A

A

V

V

A

A

W

Y

L

L

M

E

P

P

F

F

M

I

E

E

A

A

E

S

K

K

E

E

G

-

Q

-

E

Q

R

G

K

K

A

T

A

N

G

G

K

K

V

M

L

V

M
x
I

A

A

T

T

V

V

K

K

G
|
G

D
|
D

V
|
V

H
|
H

D
|
D

I
|
I

G
|
G

K
|
K

N

N

I
|
I

V
|
V

G

G

V

V

L

L

Q

Q

C

C

N

N

Y

Y

E

E

V

I

V

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

A

A

D

E

R

K

I

I

L

L

D

R

E

T

A

A

K

K

K

E

H

V

K

N

V

A

D

D

M

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

V

V

F

N

V

V

A

A

A

K

E

E

M

M

E

E

R

R

Q

Q

G

G

F

F

D

T

I

I

P

P

L

L

L
|
L

I

I

G
|
G

A
|
A

T
|
T

T

T

S

S

R

K

T

A

H

H

T

T

A

A

V

V

K

K

I

I

E

E

P

Q

A

N

Y

Y

R

-

R

S

G

G

P

P

T

T

T

V

Y

Y

V
|
V

V
x
Q

D
x
N

A
|
A

S

S

R

R

A
x
T

V

V

G

G

V

V

S

A

G

A

L

L

S

S

E

D

G

T

E

Q

R

R

D

D

R

D

I

F

I

V

A

A

E

R

T

T

R

R

A

K

E

E

Y

Y

V

E

K

T

V

V

R

R

E

I

Q

Q

Y

H

A

G

R

R

active site: D107 (= D415), H109 (= H417), S160 (= S468)
binding co-methylcobalamin: E44 (= E350), M48 (= M354), M51 (= M357), G55 (= G361), F58 (= F364), L65 (= L371), V68 (= V374), V69 (= V375), I101 (≠ M409), G106 (= G414), D107 (= D415), V108 (= V416), H109 (= H417), D110 (= D418), I111 (= I419), G112 (= G420), K113 (= K421), I115 (= I423), V116 (= V424), G152 (= G460), L153 (= L461), S154 (= S462), L156 (= L464), I157 (= I465), T158 (= T466), L181 (= L489), G183 (= G491), G184 (= G492), A185 (= A493), T186 (= T494), V207 (= V516), Q208 (≠ V517), N209 (≠ D518), A210 (= A519), T213 (≠ A522)

6bdyA Crystal structure of the meth reactivation domain bound to sinefungin (see paper)
53% identity, 36% coverage: 564:889/899 of query aligns to 5:325/326 of 6bdyA

query
sites
6bdyA

S

T

I

L

Q

E

E

A

A

A

R

R

K

D

R

N

A

D

F

F

A

A

I

F

D

D

W

W

K

Q

G

A

Y

Y

A

T

P

P

K

V

P

A

A

H

F

R

I

L

G

G

T

V

R

Q

V

E

F

V

E

E

P

A

S

S

L

I

A

E

E

T

L

L

V

R

P

N

F

Y

I

I

D
|
D

W

W

S

T

P
|
P

F

F

A

M

S

T

W

W

E

S

L

L

I

A

G

G

R

K

F

Y

P

P

Q

R

I

I

L

L

E

E

D

D

D

E

V

V

G

G

Q

V

A

E

A

A

T

Q

D

R

L

L

Y

F

R

K

D

D

A

A

R

N

A

D

M

M

L

L

D

D

K

K

V

L

V

S

E

A

E

E

K

K

W

T

F

L

G

N

A

P

K

R

G

G

V

V

I

V

G

G

F

L

W

F

P

P

A

A

Q

N

A

R

Q

V

G

G

D

D

I

I

V

E

L

I

Y

Y

T

R

D

D

E

E

T

T

R

R

V

T

A

H

E

V

F

I

S

N

R

V

L

S

H

H

T

H

L

L

R

R

Q

Q

M

T

D

E

K

K

G

T

A

G

D

-

K

-

S

-

G

-

E

-

A

-

K

F

A

A

N

N

V

Y

A

C

L

L

S

A

D

D

F

F

V

V

A

A

P

P

I

K

G

L

Q

S

G

G

-

K

A

A

D

D

Y

Y

V

I

G

G

G

A

F

F

A

A

V

V

T

T

A

G

G

G

H

L

G

E

E

E

D

D

E

A

I

L

V

A

A

D

K

A

F

F

K

E

A

A

A

Q

G

H

D

D

Y

Y

N

N

A

K

I

I

M

M

A

V

S

K

A

A

L

L

A

A

D

D

R
|
R

L

L

A

A

E
|
E

A

A

F

F

A

A

E

E

W

Y

L

L

H

H

Y

E

K

R

A

V

R

R

V

K

E

V

L

Y

W

W

G

G

Y

Y

A

A

A

P

D

N

E

E

D

N

A

L

D

S

V

N

E

E

R

E

L

L

I

I

A

R

E

E

K

N

Y

Y

Q

Q

G

G

I

I

R
|
R

P
|
P

A
|
A

P

P

G

G

Y
|
Y

P
|
P

A
|
A

Q

C

P

P

D

E

H

H

T

T

E

E

K

K

G

A

T

T

L

I

F

W

K

E

L

L

D

E

A

V

E

E

A

K

A

H

T

T

G

G

L

M

Q

K

L

L

T

T

E

E

S

S

Y

F

A

A

M

M

T

W

P

P

G

G

A

A

A

S

V

V

S

S

G

G

L

W

F

Y

F

F

S

S

H

H

R

P

Q

D

A

S

H

K

Y
|
Y

F
x
Y

G

A

V

V

G

A

K

Q

I

I

D

Q

A

R

D

D

Q

Q

V

V

E

E

D

D

Y

Y

A

A

R

R

K

K

G

G

W

M

D

S

M

V

E

T

T

E

A

V

E

E

R

R

W

W

L

L

S

A

P

P

I

N

L

L

N

G

Y

Y

D

D

binding sinefungin: D46 (= D605), P49 (= P608), R194 (= R758), E197 (= E761), R234 (= R798), P235 (= P799), A236 (= A800), Y239 (= Y803), P240 (= P804), A241 (= A805), Y289 (= Y853), Y290 (≠ F854)

1mskA Methionine synthase (activation domain) (see paper)
53% identity, 36% coverage: 564:889/899 of query aligns to 5:325/327 of 1mskA

query
sites
1mskA

S

T

I

L

Q

E

E

A

A

A

R

R

K

D

R

N

A

D

F

F

A

A

I

F

D

D

W

W

K

Q

G

A

Y

Y

A

T

P

P

K

V

P

A

A

H

F

R

I

L

G

G

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R

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V

E

F

V

E

E

P

A

S

S

L

I

A

E

E

T

L

L

V

R

P

N

F

Y

I

I

D
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D

W

W

S

T

P

P

F

F

A

M

S

T

W

W

E

S

L

L

I

A

G

G

R

K

F

Y

P

P

Q

R

I

I

L

L

E

E

D

D

D

E

V

V

G

G

Q

V

A

E

A

A

T

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D

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L

L

Y

F

R

K

D

D

A

A

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N

A

D

M

M

L

L

D

D

K

K

V

L

V

S

E

A

E

E

K

K

W

T

F

L

G

N

A

P

K

R

G

G

V

V

I

V

G

G

F

L

W

F

P

P

A

A

Q

N

A

R

Q

V

G

G

D

D

I

I

V

E

L

I

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Y

T

R

D

D

E

E

T

T

R

R

V

T

A

H

E

V

F

I

S

N

R

V

L

S

H

H

T

H

L

L

R

R

Q

Q

M

T

D

E

K

K

G

T

A

G

D

-

K

-

S

-

G

-

E

-

A

-

K

F

A

A

N

N

V

Y

A

C

L

L

S

A

D

D

F

F

V

V

A

A

P

P

I

K

G

L

Q

S

G

G

-

K

A

A

D

D

Y

Y

V

I

G

G

G

A

F

F

A

A

V

V

T

T

A

G

G

G

H

L

G

E

E

E

D

D

E

A

I

L

V

A

A

D

K

A

F

F

K

E

A

A

A

Q

G

H

D

D

Y

Y

N

N

A

K

I

I

M

M

A

V

S

K

A

A

L

L

A

A

D

D

R
|
R

L

L

A

A

E
|
E

A

A

F

F

A

A

E

E

W

Y

L

L

H

H

Y

E

K

R

A

V

R

R

V

K

E

V

L

Y

W

W

G

G

Y

Y

A

A

A

P

D

N

E

E

D

N

A

L

D

S

V

N

E

E

R

E

L

L

I
|
I

A

R

E

E

K

N

Y

Y

Q

Q

G

G

I

I

R
|
R

P
|
P

A
|
A

P

P

G

G

Y
|
Y

P
|
P

A
|
A

Q

C

P

P