SitesBLAST
Comparing CCNA_02725 FitnessBrowser__Caulo:CCNA_02725 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
37% identity, 96% coverage: 1:535/555 of query aligns to 19:572/578 of 5nccA
- active site: R347 (≠ G324), L420 (≠ V385), I421 (≠ C386), S507 (≠ I470), A509 (≠ H472), G552 (= G515), Q553 (≠ N516)
- binding flavin-adenine dinucleotide: G30 (= G12), G32 (= G14), T33 (≠ S15), A34 (= A16), L53 (= L35), E54 (= E36), A55 (= A37), F74 (≠ I56), W80 (= W64), A98 (≠ P82), G100 (= G84), G105 (= G89), S106 (= S90), N110 (= N94), A111 (≠ G95), T112 (≠ M96), L113 (≠ I97), V238 (≠ T221), A278 (= A257), H282 (≠ Q261), L286 (≠ I265), N508 (≠ Y471), Q553 (≠ N516), T554 (= T517), G555 (≠ N518), V558 (≠ T521)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
37% identity, 96% coverage: 1:535/555 of query aligns to 79:639/654 of A0A248QE08
- TA 93:94 (≠ SA 15:16) binding
- E114 (= E36) binding
- L162 (≠ G86) binding
- S166 (= S90) binding
- NATL 170:173 (≠ NGMI 94:97) binding
- V298 (≠ T221) binding
- C432 (≠ L342) binding
- R451 (≠ H362) binding
- Y466 (≠ E377) binding
- Q486 (≠ H384) binding
- G622 (≠ N518) binding
6yrvAAA structure of fap after illumination at 100k (see paper)
37% identity, 96% coverage: 1:535/555 of query aligns to 3:563/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H362), N499 (≠ Y471)
- binding flavin-adenine dinucleotide: G14 (= G12), G16 (= G14), T17 (≠ S15), A18 (= A16), L37 (= L35), E38 (= E36), A39 (= A37), F58 (≠ I56), W64 (= W64), A82 (≠ P82), G89 (= G89), S90 (= S90), N94 (= N94), A95 (≠ G95), T96 (≠ M96), L97 (≠ I97), M191 (≠ T190), V222 (≠ T221), C264 (≠ S256), A265 (= A257), G266 (= G258), H269 (≠ Q261), N499 (≠ Y471), A534 (= A506), Q544 (≠ N516), T545 (= T517), G546 (≠ N518)
- binding heptadecane: V377 (= V364), G379 (≠ A366), M380 (≠ I367), G386 (≠ K373), T389 (≠ V376), Y390 (≠ E377), F393 (vs. gap), T408 (= T382), Q410 (≠ H384)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
40% identity, 96% coverage: 3:537/555 of query aligns to 2:457/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G12), G13 (= G14), S14 (= S15), A15 (= A16), E35 (= E36), A36 (= A37), W47 (= W64), P65 (= P82), G67 (= G84), V180 (≠ T221), A214 (= A257), G215 (= G258), A218 (≠ Q261), T270 (≠ K322), Y391 (= Y471), A424 (= A506), I435 (≠ T517), N436 (= N518)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
37% identity, 96% coverage: 1:533/555 of query aligns to 9:527/527 of 2jbvA
- active site: I333 (≠ S344), P377 (≠ V385), N378 (≠ C386), V464 (≠ I470), H466 (= H472), V509 (≠ G515), N510 (= N516)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G14), S23 (= S15), E44 (= E36), A45 (= A37), W71 (= W64), A90 (≠ G84), G95 (= G89), C96 (≠ S90), H99 (≠ I93), N100 (= N94), S101 (≠ G95), I103 (= I97), R231 (≠ C220), A232 (≠ T221), T269 (≠ A257), G270 (= G258), D273 (≠ Q261), V464 (≠ I470), Y465 (= Y471), H466 (= H472), D499 (= D505), A500 (= A506), N510 (= N516), P511 (≠ T517), N512 (= N518), V515 (≠ T521)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
37% identity, 96% coverage: 1:533/555 of query aligns to 9:527/532 of 4mjwA
- active site: I333 (≠ S344), P377 (≠ V385), N378 (≠ C386), V464 (≠ I470), H466 (= H472), V509 (≠ G515), N510 (= N516)
- binding flavin-adenine dinucleotide: G20 (= G12), G22 (= G14), S23 (= S15), E44 (= E36), A45 (= A37), W71 (= W64), R89 (= R83), A90 (≠ G84), G95 (= G89), C96 (≠ S90), H99 (≠ I93), N100 (= N94), S101 (≠ G95), I103 (= I97), R231 (≠ C220), A232 (≠ T221), T269 (≠ A257), G270 (= G258), D273 (≠ Q261), Y465 (= Y471), H466 (= H472), A500 (= A506), N510 (= N516), P511 (≠ T517), N512 (= N518), V515 (≠ T521)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
37% identity, 96% coverage: 1:533/555 of query aligns to 9:527/530 of 3ljpA
- active site: I333 (≠ S344), P377 (≠ V385), N378 (≠ C386), A464 (≠ I470), H466 (= H472), V509 (≠ G515), N510 (= N516)
- binding dihydroflavine-adenine dinucleotide: G22 (= G14), S23 (= S15), E44 (= E36), A45 (= A37), W71 (= W64), R89 (= R83), A90 (≠ G84), G95 (= G89), C96 (≠ S90), H99 (≠ I93), N100 (= N94), S101 (≠ G95), I103 (= I97), A232 (≠ T221), T269 (≠ A257), D273 (≠ Q261), Y465 (= Y471), H466 (= H472), D499 (= D505), A500 (= A506), N510 (= N516), P511 (≠ T517), N512 (= N518), V515 (≠ T521)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
30% identity, 95% coverage: 6:533/555 of query aligns to 2:562/565 of 5oc1A
- active site: V339 (= V325), N413 (≠ Q387), A414 (vs. gap), I499 (= I470), H501 (= H472), A544 (≠ G515), H545 (≠ N516)
- binding 4-methoxybenzoic acid: Y91 (≠ G95), I356 (≠ F332), I390 (≠ V364), F396 (≠ D370), T412 (≠ C386), I499 (= I470), H501 (= H472), H545 (≠ N516)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), N11 (≠ S15), A12 (= A16), E32 (= E36), A33 (= A37), W60 (= W64), P78 (= P82), G80 (= G84), G85 (= G89), S86 (= S90), H90 (≠ N94), Y91 (≠ G95), V93 (≠ I97), V230 (≠ T221), S270 (= S256), A271 (= A257), G272 (= G258), F500 (≠ Y471), H545 (≠ N516), T546 (= T517), Q547 (≠ N518), I550 (≠ T521)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
29% identity, 95% coverage: 6:533/555 of query aligns to 2:562/565 of 3fimB
- active site: V339 (= V325), N413 (≠ Q387), A414 (vs. gap), I499 (= I470), H501 (= H472), A544 (≠ G515), H545 (≠ N516)
- binding flavin-adenine dinucleotide: G8 (= G12), N11 (≠ S15), A12 (= A16), E32 (= E36), A33 (= A37), W60 (= W64), P78 (= P82), G80 (= G84), G85 (= G89), S86 (= S90), H90 (≠ N94), Y91 (≠ G95), V93 (≠ I97), V230 (≠ T221), S270 (= S256), A271 (= A257), F500 (≠ Y471), H501 (= H472), H545 (≠ N516), T546 (= T517), Q547 (≠ N518), I550 (≠ T521)
3q9tA Crystal structure analysis of formate oxidase (see paper)
30% identity, 95% coverage: 6:535/555 of query aligns to 7:572/577 of 3q9tA
- active site: A335 (vs. gap), D422 (≠ C386), A508 (≠ I470), H510 (= H472), C552 (≠ G515), R553 (≠ N516)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl(2R,3S,4S)-5-(8-formyl-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)-2,3,4-trihydroxypentyl dihydrogendiphosphate: G15 (= G14), T16 (≠ S15), E37 (= E36), A38 (= A37), W65 (= W64), T85 (vs. gap), R86 (= R83), G87 (= G84), G92 (= G89), S93 (= S90), N97 (= N94), Y98 (≠ G95), F99 (≠ M96), T100 (≠ I97), S229 (≠ T221), S265 (= S256), Q266 (≠ A257), E270 (≠ Q261), F509 (≠ Y471), D542 (= D505), A543 (= A506), R553 (≠ N516), I554 (≠ T517), Q555 (≠ N518), V558 (≠ T521)
5zu2A Effect of mutation (r554a) on fad modification in aspergillus oryzae rib40formate oxidase (see paper)
30% identity, 95% coverage: 6:535/555 of query aligns to 7:572/577 of 5zu2A
- binding flavin-adenine dinucleotide: G15 (= G14), T16 (≠ S15), E37 (= E36), A38 (= A37), W65 (= W64), T85 (vs. gap), R86 (= R83), G87 (= G84), G92 (= G89), S93 (= S90), N97 (= N94), Y98 (≠ G95), T100 (≠ I97), S229 (≠ T221), S265 (= S256), Q266 (≠ A257), E270 (≠ Q261), F509 (≠ Y471), D542 (= D505), A543 (= A506), A553 (≠ N516), I554 (≠ T517), Q555 (≠ N518), V558 (≠ T521)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
33% identity, 96% coverage: 1:532/555 of query aligns to 1:527/531 of E4QP00
- V101 (≠ I93) mutation to H: Abolishes activity.
- M103 (≠ G95) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (= V385) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ Q387) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ I470) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ Y471) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H472) mutation to A: Abolishes activity.
- N511 (= N516) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
33% identity, 95% coverage: 6:532/555 of query aligns to 2:523/525 of 4udqA
- active site: L331 (≠ F341), F364 (≠ C386), W365 (≠ Q387), V461 (≠ I470), H463 (= H472), A506 (≠ G515), N507 (= N516)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), T11 (≠ S15), A12 (= A16), E32 (= E36), A33 (= A37), W64 (= W67), G88 (= G84), G93 (= G89), G94 (≠ S90), N98 (= N94), M99 (≠ G95), V101 (≠ I97), V229 (≠ T221), T261 (≠ S256), A262 (= A257), W462 (≠ Y471), H463 (= H472), A497 (= A506), N507 (= N516), T508 (= T517), N509 (= N518), T512 (= T521)
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
34% identity, 94% coverage: 7:529/555 of query aligns to 3:501/508 of 4ha6A
- active site: F360 (≠ V385), G361 (≠ C386), H444 (≠ I470), H446 (= H472), G487 (= G515), P488 (≠ N516)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), S11 (= S15), A12 (= A16), E32 (= E36), A33 (= A37), W58 (= W64), R77 (= R83), G78 (= G84), G83 (= G89), S84 (= S90), L87 (≠ I93), H88 (≠ N94), A89 (≠ G95), M90 (= M96), G91 (≠ I97), V218 (≠ T221), A251 (= A257), G252 (= G258), E255 (≠ Q261), H445 (≠ Y471), A478 (= A506), P488 (≠ N516), I489 (≠ T517), H490 (≠ N518)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (≠ G95), S314 (≠ L342), H444 (≠ I470), H446 (= H472)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
34% identity, 94% coverage: 7:529/555 of query aligns to 3:501/509 of 3t37A
- active site: F360 (≠ V385), G361 (≠ C386), H444 (≠ I470), H446 (= H472), G487 (= G515), P488 (≠ N516)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), S11 (= S15), A12 (= A16), E32 (= E36), A33 (= A37), W58 (= W64), R77 (= R83), G78 (= G84), R79 (≠ K85), G83 (= G89), S84 (= S90), H88 (≠ N94), A89 (≠ G95), G91 (≠ I97), R217 (≠ C220), V218 (≠ T221), A251 (= A257), E255 (≠ Q261), H445 (≠ Y471), A478 (= A506), P488 (≠ N516), I489 (≠ T517), H490 (≠ N518)
8bxlB Patulin synthase from penicillium expansum
31% identity, 96% coverage: 3:535/555 of query aligns to 11:589/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G12), G22 (= G14), T23 (≠ S15), A24 (= A16), E44 (= E36), A45 (= A37), W80 (= W64), G100 (= G84), G105 (= G89), S106 (= S90), R109 (≠ I93), N110 (= N94), Y111 (≠ G95), A113 (≠ I97), L253 (≠ C220), A254 (≠ T221), A288 (= A257), Q292 (= Q261), F525 (≠ Y471), D559 (= D505), A560 (= A506), H570 (≠ N516), P571 (≠ T517), Q572 (≠ N518), L575 (≠ T521)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
30% identity, 95% coverage: 6:532/555 of query aligns to 41:597/602 of Q3L245
- N100 (≠ G65) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I93) modified: Tele-8alpha-FAD histidine
- N344 (= N294) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H472) active site, Proton acceptor
- H581 (≠ N516) active site
Sites not aligning to the query:
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
30% identity, 95% coverage: 6:532/555 of query aligns to 16:572/577 of 4h7uA
- active site: A343 (≠ K318), V426 (≠ C386), Y510 (≠ I470), H512 (= H472), A555 (≠ G515), H556 (≠ N516)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G12), G24 (= G14), T25 (≠ S15), A26 (= A16), E46 (= E36), A47 (= A37), W74 (= W64), G99 (= G89), C100 (≠ S90), H103 (≠ I93), N104 (= N94), G105 (= G95), V107 (≠ I97), L242 (≠ C220), V243 (≠ T221), G282 (≠ A257), G283 (= G258), A286 (≠ Q261), H512 (= H472), A546 (= A506), H556 (≠ N516), T557 (= T517), Q558 (≠ N518), V561 (≠ T521)
6xuuA Crystallographic structure of oligosaccharide dehydrogenase from pycnoporus cinnabarinus, glucose-bound form (see paper)
28% identity, 95% coverage: 4:533/555 of query aligns to 13:586/589 of 6xuuA
- binding beta-D-glucopyranose: E35 (= E26), N67 (≠ K59), H83 (≠ P82), N104 (≠ A103), D108 (= D107), S139 (≠ H133), A140 (= A134), N142 (≠ G136), T166 (≠ S149), D169 (vs. gap), T188 (≠ A168), W213 (vs. gap), A225 (= A202), Y226 (= Y203), Q329 (≠ D300), N330 (≠ V301), A331 (≠ C302), Q357 (≠ M330), F414 (vs. gap), K474 (≠ E432), N517 (≠ E468), G520 (vs. gap), A522 (vs. gap), V524 (≠ I470), H526 (= H472), H569 (≠ N516)
- binding flavin-adenine dinucleotide: G21 (= G12), G23 (= G14), L24 (≠ S15), T25 (≠ A16), E45 (= E36), A46 (= A37), F66 (vs. gap), W72 (= W64), H83 (≠ P82), G85 (= G84), G90 (= G89), S91 (= S90), N95 (= N94), G96 (= G95), A97 (≠ M96), A98 (≠ I97), M244 (≠ C220), A245 (≠ T221), A286 (= A257), Q290 (= Q261), A525 (≠ Y471), A559 (= A506), H569 (≠ N516), L570 (≠ T517), S571 (≠ N518), L574 (≠ T521)
- binding alpha-D-glucopyranose: G96 (= G95), F414 (vs. gap), W428 (≠ C386)
6xutA Crystallographic structure of oligosaccharide dehydrogenase from pycnoporus cinnabarinus, ligand-free form (see paper)
28% identity, 95% coverage: 4:533/555 of query aligns to 13:586/589 of 6xutA
- binding flavin-adenine dinucleotide: G21 (= G12), G23 (= G14), L24 (≠ S15), T25 (≠ A16), E45 (= E36), A46 (= A37), F66 (vs. gap), W72 (= W64), H83 (≠ P82), G85 (= G84), G90 (= G89), S91 (= S90), N95 (= N94), G96 (= G95), A97 (≠ M96), A98 (≠ I97), M244 (≠ C220), A245 (≠ T221), A286 (= A257), G287 (= G258), Q290 (= Q261), A525 (≠ Y471), H526 (= H472), A559 (= A506), H569 (≠ N516), L570 (≠ T517), S571 (≠ N518), L574 (≠ T521)
Query Sequence
>CCNA_02725 FitnessBrowser__Caulo:CCNA_02725
MASERFDYIVIGAGSAGCVLAARLTEDPNIKVLLLEAGGKNKSILVKMPAGVGQLIKDKG
EQNWGFWTEAEPHLDNRKLWWPRGKGLGGSSAINGMIYIRGHARDYDQWRQMGLTGWSYS
EVLPYFKRSETHHAGGDAYHGGSGPLHVSKGESDSPFYSTLVEAGRQAGHKTTKDFNGYQ
QEGFGPYDLTIRDGQRWSAAMAYLNQALSRPNLTCVTEACTTRIILDKRRAVGVEYVVGK
SREKQVAYADAEVLLSAGAVQSPQILQLSGIGAAEDLAPHGIAVAHESKGVGANLQDHLD
VCVSWTAKNLKTAYSANKGLNKLGVGMNYMFFGKGLGRQQFLESGAFLKSRPDLDRPDLQ
IHGVLAIMQDHGKVVVEKDGFTLHVCQLRPESRGKVGLRSADPFDDPTILGNYLATEEDR
RAIREGVRIARETVAQAAFDPYRDAEYAPGADVKSDADLDAWIRSKAETIYHPVGTCRMG
VAGDPMAVVDDQLRVQGVQGLRVIDASVMPTLIGGNTNAPTIMIAERAADLIRGKTPAAP
LDVPVYEDGRAVAAE
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory