SitesBLAST
Comparing CCNA_03103 FitnessBrowser__Caulo:CCNA_03103 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4y0aA Shikimate kinase from acinetobacter baumannii in complex with shikimate (see paper)
41% identity, 75% coverage: 33:181/200 of query aligns to 10:159/179 of 4y0aA
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M17 (= M40), D40 (= D63), I51 (= I74), F55 (= F78), F63 (= F86), R64 (= R87), G85 (= G108), G86 (= G109), G87 (= G110), L126 (= L149), R143 (= R166)
- binding sulfate ion: M17 (= M40), G18 (= G41), A19 (≠ V42), G20 (= G43), K21 (= K44), T22 (≠ S45), P110 (≠ D133), V111 (= V134), E112 (= E135), R117 (= R140), R124 (= R147), R137 (≠ T160), Q159 (≠ D181)
Sites not aligning to the query:
1zuiA Structural basis for shikimate-binding specificity of helicobacter pylori shikimate kinase (see paper)
37% identity, 69% coverage: 34:171/200 of query aligns to 3:137/158 of 1zuiA
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: M9 (= M40), D32 (= D63), V43 (≠ I74), F47 (= F78), F55 (= F86), R56 (= R87), G78 (= G108), G79 (= G109), G80 (= G110), E111 (≠ D145), K112 (≠ N146), R113 (= R147), L116 (≠ V150), R129 (≠ A163)
3n2eA Crystal structure of helicobactor pylori shikimate kinase in complex with nsc162535 (see paper)
37% identity, 69% coverage: 34:171/200 of query aligns to 4:140/168 of 3n2eA
- binding 7-amino-4-hydroxy-3-[(E)-(5-hydroxy-7-sulfonaphthalen-2-yl)diazenyl]naphthalene-2-sulfonic acid: F9 (≠ L39), M10 (= M40), R45 (≠ S75), F48 (= F78), E53 (= E83), R57 (= R87), G80 (= G109), G81 (= G110), V83 (≠ F112), M84 (≠ V113), E112 (≠ D145), K115 (≠ N146), R116 (= R147), R132 (≠ A163), L135 (≠ R166), Y136 (= Y167)
3mufA Shikimate kinase from helicobacter pylori in complex with shikimate-3- phosphate and adp (see paper)
37% identity, 69% coverage: 34:171/200 of query aligns to 3:139/160 of 3mufA
- binding adenosine-5'-diphosphate: G10 (= G41), S11 (≠ V42), G12 (= G43), K13 (= K44), S14 (= S45), S15 (= S46), M98 (≠ A132), R106 (= R140), E111 (vs. gap), R115 (= R147)
- binding shikimate-3-phosphate: M9 (= M40), K13 (= K44), D32 (= D63), V43 (≠ I74), F55 (= F86), R56 (= R87), G78 (= G108), G79 (= G109), G80 (= G110), R115 (= R147), L117 (= L149), R131 (≠ A163)
Sites not aligning to the query:
2iywA Shikimate kinase from mycobacterium tuberculosis in complex with mgatp, open lid (conf. B) (see paper)
38% identity, 81% coverage: 35:196/200 of query aligns to 5:166/178 of 2iywA
- binding adenosine-5'-triphosphate: L9 (= L39), P10 (≠ M40), G11 (= G41), S12 (≠ V42), G13 (= G43), K14 (= K44), S15 (= S45), T16 (≠ S46), R109 (= R140), T149 (= T179), R152 (≠ T182), N153 (≠ P183), P154 (≠ H184)
2iyvA Shikimate kinase from mycobacterium tuberculosis in complex with adp, open lid (conf. B) (see paper)
38% identity, 81% coverage: 35:196/200 of query aligns to 5:166/179 of 2iyvA
- binding adenosine-5'-diphosphate: P10 (≠ M40), G11 (= G41), S12 (≠ V42), G13 (= G43), K14 (= K44), S15 (= S45), T16 (≠ S46), R109 (= R140), T149 (= T179), R152 (≠ T182), N153 (≠ P183), P154 (≠ H184)
1zyuA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and amppcp at 2.85 angstrom resolution (see paper)
38% identity, 81% coverage: 35:196/200 of query aligns to 5:166/168 of 1zyuA
- binding phosphomethylphosphonic acid adenylate ester: P10 (≠ M40), G11 (= G41), S12 (≠ V42), G13 (= G43), K14 (= K44), S15 (= S45), T16 (≠ S46), R109 (= R140), R116 (= R147), T149 (= T179), R152 (≠ T182), N153 (≠ P183), P154 (≠ H184)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: P10 (≠ M40), D33 (= D63), R57 (= R87), G79 (= G109), G80 (= G110), P117 (= P148), L118 (= L149), R135 (= R166)
4bqsA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with adp and a shikimic acid derivative. (see paper)
38% identity, 81% coverage: 35:196/200 of query aligns to 5:166/169 of 4bqsA
- binding adenosine-5'-diphosphate: P10 (≠ M40), G11 (= G41), S12 (≠ V42), G13 (= G43), K14 (= K44), S15 (= S45), T16 (≠ S46), R109 (= R140), R152 (≠ T182), P154 (≠ H184)
- binding (1R,6R,10S)-6,10-dihydroxy-2-oxabicyclo[4.3.1]deca-4(Z),7-diene-8-carboxylic acid: D33 (= D63), I44 (= I74), F48 (= F78), F56 (= F86), R57 (= R87), G78 (= G108), G79 (= G109), G80 (= G110), P117 (= P148), L118 (= L149), R135 (= R166)
2iyyA Shikimate kinase from mycobacterium tuberculosis in complex with shikimate-3-phosphate and so4 (see paper)
38% identity, 81% coverage: 35:196/200 of query aligns to 5:166/169 of 2iyyA
- binding magnesium ion: E37 (= E67), S43 (= S73), I44 (= I74)
- binding phosphate ion: P10 (≠ M40), K14 (= K44), D33 (= D63), G78 (= G108), G79 (= G109)
- binding shikimate-3-phosphate: P10 (≠ M40), K14 (= K44), D33 (= D63), I44 (= I74), R57 (= R87), G78 (= G108), G79 (= G109), G80 (= G110), L118 (= L149), R135 (= R166)
2iyxA Shikimate kinase from mycobacterium tuberculosis in complex with shikimate and so4 (see paper)
38% identity, 81% coverage: 35:196/200 of query aligns to 5:166/169 of 2iyxA
- binding magnesium ion: E37 (= E67), S43 (= S73)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: P10 (≠ M40), D33 (= D63), I44 (= I74), F48 (= F78), F56 (= F86), R57 (= R87), G78 (= G108), G79 (= G109), G80 (= G110), P117 (= P148), L118 (= L149), R135 (= R166)
3bafA Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with amp-pnp
38% identity, 81% coverage: 35:195/200 of query aligns to 5:165/165 of 3bafA
- binding phosphoaminophosphonic acid-adenylate ester: P10 (≠ M40), G11 (= G41), S12 (≠ V42), G13 (= G43), K14 (= K44), S15 (= S45), T16 (≠ S46), G79 (= G109), R109 (= R140), R116 (= R147), R152 (≠ T182), P154 (≠ H184)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D63), R57 (= R87), G78 (= G108), G79 (= G109), G80 (= G110), R135 (= R166)
2dfnA Structure of shikimate kinase from mycobacterium tuberculosis complexed with adp and shikimate at 1.9 angstrons of resolution (see paper)
38% identity, 81% coverage: 35:195/200 of query aligns to 5:165/165 of 2dfnA
- binding adenosine-5'-diphosphate: P10 (≠ M40), G11 (= G41), S12 (≠ V42), G13 (= G43), K14 (= K44), S15 (= S45), T16 (≠ S46), R109 (= R140), R116 (= R147), R152 (≠ T182), N153 (≠ P183), P154 (≠ H184)
- binding chloride ion: P10 (≠ M40), K14 (= K44), G79 (= G109)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: P10 (≠ M40), D33 (= D63), I44 (= I74), F48 (= F78), F56 (= F86), R57 (= R87), G78 (= G108), G79 (= G109), G80 (= G110), L118 (= L149), R135 (= R166)
1we2A Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with mgadp and shikimic acid (see paper)
38% identity, 81% coverage: 35:195/200 of query aligns to 5:165/165 of 1we2A
- binding adenosine-5'-diphosphate: L9 (= L39), P10 (≠ M40), G11 (= G41), S12 (≠ V42), G13 (= G43), K14 (= K44), S15 (= S45), T16 (≠ S46), R109 (= R140), R116 (= R147), T149 (= T179), R152 (≠ T182), N153 (≠ P183), P154 (≠ H184)
- binding 3-dehydroshikimate: D33 (= D63), I44 (= I74), F48 (= F78), F56 (= F86), R57 (= R87), G78 (= G108), G79 (= G109), G80 (= G110), L118 (= L149), R135 (= R166)
1u8aA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and adp at 2.15 angstrom resolution (see paper)
38% identity, 81% coverage: 35:195/200 of query aligns to 5:163/163 of 1u8aA
- binding adenosine-5'-diphosphate: P10 (≠ M40), G11 (= G41), S12 (≠ V42), G13 (= G43), K14 (= K44), S15 (= S45), T16 (≠ S46), R109 (= R140), R114 (= R147), R150 (≠ T182), N151 (≠ P183), P152 (≠ H184)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: P10 (≠ M40), D33 (= D63), I44 (= I74), F48 (= F78), F56 (= F86), R57 (= R87), G78 (= G108), G79 (= G109), G80 (= G110), P115 (= P148), L116 (= L149), R133 (= R166)
P10880 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see paper)
28% identity, 82% coverage: 34:196/200 of query aligns to 5:169/173 of P10880
- C13 (≠ V42) mutation to S: 66% of wild-type activity. Increase in substrates affinity.
- K15 (= K44) mutation to M: Loss of activity. Increased thermostability.
- D34 (= D63) mutation to N: Loss of activity.
- C162 (≠ E189) mutation to S: No effect on activity and substrates affinity.
2shkB The three-dimensional structure of shikimate kinase from erwinia chrysanthemi complexed with adp (see paper)
34% identity, 58% coverage: 34:149/200 of query aligns to 5:118/162 of 2shkB
Sites not aligning to the query:
1e6cA K15m mutant of shikimate kinase from erwinia chrysanthemi (see paper)
28% identity, 82% coverage: 34:196/200 of query aligns to 5:169/170 of 1e6cA
1shkA The three-dimensional structure of shikimate kinase from erwinia chrysanthemi (see paper)
35% identity, 55% coverage: 34:142/200 of query aligns to 5:112/158 of 1shkA
Sites not aligning to the query:
P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
33% identity, 59% coverage: 27:143/200 of query aligns to 858:974/1583 of P07547
Sites not aligning to the query:
- 44:46 binding
- 81:84 binding
- 114:116 binding
- 119 binding
- 139:140 binding
- 161 binding
- 179:182 binding
- 190 binding
- 194 binding
- 271 binding
- 287 binding
6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
26% identity, 81% coverage: 32:192/200 of query aligns to 843:1003/1555 of 6hqvA
Sites not aligning to the query:
- active site: 123, 145, 187, 243, 253, 257, 261, 264, 268, 280
- binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1036, 1060, 1062, 1094, 1153, 1181, 1214, 1224, 1232, 1242, 1243, 1246
- binding glutamic acid: 139, 145, 187, 243, 257, 260, 261, 264, 280
- binding nicotinamide-adenine-dinucleotide: 42, 44, 45, 49, 74, 75, 76, 79, 107, 108, 109, 112, 132, 133, 135, 139, 140, 145, 154, 155, 172, 175, 176, 177, 180, 187, 243, 280
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 409, 413, 483, 562, 563, 593, 1269, 1277, 1279, 1321, 1322, 1323, 1327, 1348, 1368, 1526, 1530
- binding zinc ion: 187, 264, 280
Query Sequence
>CCNA_03103 FitnessBrowser__Caulo:CCNA_03103
MTETDQTPDTAPEAAPEVAPIVSDDLAPLRAKTIVLVGLMGVGKSSVGRRLANVLGLPFR
DADNEVEAAAGRSISEIFAELGEPAFRDGERRVIARLLDEPPHVLATGGGAFVNAETRAL
INEKAVSVWLKADVELLARRVSRKDNRPLVRGKDPVKVLTELAEARYPAYAEAQVHVETG
DTPHMVAVEAILTALRQAHA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory