SitesBLAST
Comparing CCNA_03229 FitnessBrowser__Caulo:CCNA_03229 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 12 hits to proteins with known functional sites (download)
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
23% identity, 84% coverage: 39:399/429 of query aligns to 3:359/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (= I43), G8 (= G44), G10 (= G46), V11 (≠ F47), I12 (≠ T48), V30 (≠ L63), E31 (= E64), K32 (≠ A65), E38 (≠ A71), A39 (= A72), S40 (= S73), A43 (≠ N76), G45 (= G78), L46 (≠ Q79), V171 (≠ A213), G200 (= G241), G201 (≠ D242), W203 (≠ L244), G298 (= G343), R300 (≠ L345), P301 (≠ G346), Y326 (≠ S369), R327 (≠ G370), N328 (≠ Q371), G329 (= G372), I330 (≠ V373)
3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
25% identity, 49% coverage: 186:397/429 of query aligns to 147:356/364 of 3if9A
- binding flavin-adenine dinucleotide: P173 (≠ R212), V174 (≠ A213), S202 (≠ G241), G203 (vs. gap), W205 (vs. gap), F209 (≠ L245), G300 (= G343), R302 (≠ L345), H327 (≠ Y368), F328 (≠ S369), R329 (≠ G370), N330 (≠ Q371), G331 (= G372), I332 (≠ V373)
- binding glycolic acid: Y246 (≠ F288), R302 (≠ L345), R329 (≠ G370)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 34, 35, 42, 43, 46, 47, 48, 49
1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
25% identity, 49% coverage: 186:397/429 of query aligns to 147:356/364 of 1ng3A
- binding acetylamino-acetic acid: Y246 (≠ F288), R302 (≠ L345), R329 (≠ G370)
- binding flavin-adenine dinucleotide: V174 (≠ A213), S202 (≠ G241), G203 (vs. gap), W205 (vs. gap), F209 (≠ L245), G300 (= G343), R302 (≠ L345), H327 (≠ Y368), R329 (≠ G370), N330 (≠ Q371), G331 (= G372), I332 (≠ V373)
- binding phosphate ion: R254 (= R300)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49
- binding phosphate ion: 89
O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
25% identity, 49% coverage: 186:397/429 of query aligns to 147:356/369 of O31616
- V174 (≠ A213) binding
- H244 (≠ S286) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
- R302 (≠ L345) binding
- 327:333 (vs. 368:374, 14% identical) binding
- R329 (≠ G370) binding
Sites not aligning to the query:
- 14:15 binding
- 34:35 binding
- 42:43 binding
- 47:49 binding
- 51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
- 54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
25% identity, 47% coverage: 185:385/429 of query aligns to 143:349/374 of 1y56B
- active site: H224 (≠ N261), P239 (≠ A281), G305 (= G343), M338 (= M374)
- binding flavin-adenine dinucleotide: E170 (≠ R212), V171 (≠ A213), T200 (≠ G241), N201 (≠ D242), W203 (vs. gap), G305 (= G343), Y306 (≠ T344), Y307 (≠ L345), G334 (= G370), H335 (≠ Q371), G336 (= G372), F337 (≠ V373), M338 (= M374)
- binding flavin mononucleotide: I260 (≠ L302), R301 (≠ H339), W303 (= W341)
Sites not aligning to the query:
- active site: 44, 47, 48
- binding flavin-adenine dinucleotide: 11, 13, 14, 15, 33, 34, 35, 42, 43, 45, 46, 47, 49
- binding flavin mononucleotide: 44, 45
S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
25% identity, 48% coverage: 186:389/429 of query aligns to 147:348/369 of S5FMM4
- S202 (≠ G241) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
- I332 (≠ V373) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
- M342 (≠ L383) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.
Sites not aligning to the query:
- 51 G→S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
- 54 A→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
- 81 K→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
2gagB Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
24% identity, 51% coverage: 192:411/429 of query aligns to 174:393/403 of 2gagB
- binding flavin-adenine dinucleotide: V195 (≠ A213), G224 (= G241), A225 (≠ D242), H227 (vs. gap), L231 (= L245), L246 (= L269), G352 (≠ S369), T353 (≠ G370), G354 (≠ Q371), G355 (= G372), F356 (≠ V373), K357 (≠ M374)
- binding flavin mononucleotide: V250 (≠ A273), E278 (≠ R300), R321 (≠ H339), W323 (= W341)
- binding 2-furoic acid: M263 (≠ D285), Y270 (= Y292), K357 (≠ M374)
- binding sulfite ion: K276 (≠ D298)
Sites not aligning to the query:
- active site: 61, 64, 65
- binding flavin-adenine dinucleotide: 26, 28, 29, 30, 51, 52, 58, 59, 60, 62, 63, 64, 66
- binding flavin mononucleotide: 61, 62, 171
- binding 2-furoic acid: 64, 66, 68, 401
- binding sulfite ion: 170
Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
27% identity, 49% coverage: 185:395/429 of query aligns to 167:391/824 of Q8GAI3
Sites not aligning to the query:
- 66 mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
- 67 H→A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.
Q50LF2 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain U-96) (see 4 papers)
23% identity, 51% coverage: 192:411/429 of query aligns to 176:395/405 of Q50LF2
- V197 (≠ A213) binding
- H270 (≠ V290) mutation to A: 10-fold decrease in catalytic efficiency.
- Y272 (= Y292) mutation to A: 13000-fold decrease in catalytic efficiency.; mutation to F: 130-fold decrease in catalytic efficiency.
- G354 (≠ S369) binding
- G357 (= G372) binding
- K359 (≠ M374) binding ; mutation K->A,D: Loss of activity.; mutation to R: Retains 0.07% of wild-type activity. Shows higher apparent KM for sarcosine.
Sites not aligning to the query:
- 31 binding
- 32 binding
- 53 binding
- 61 binding
- 62 binding
- 66 binding
- 68 binding
- 172 K→A: Retains 39% of wild-type activity.; K→D: Retains 32% of wild-type activity.; K→R: Retains 58% of wild-type activity.
- 173 modified: Tele-8alpha-FMN histidine
3ad8B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with pyrrole 2-carboxylate (see paper)
23% identity, 51% coverage: 192:411/429 of query aligns to 175:394/404 of 3ad8B
- active site: G326 (= G343), K358 (≠ M374)
- binding flavin-adenine dinucleotide: V196 (≠ A213), G225 (= G241), A226 (≠ D242), H228 (≠ L244), L247 (= L269), G353 (≠ S369), T354 (≠ G370), G355 (≠ Q371), G356 (= G372), F357 (≠ V373), K358 (≠ M374)
- binding flavin mononucleotide: V251 (≠ A273), E279 (≠ R300), R322 (≠ H339), W324 (= W341)
- binding pyrrole-2-carboxylate: M264 (≠ D285), Y271 (= Y292), T354 (≠ G370), K358 (≠ M374)
Sites not aligning to the query:
- active site: 62, 65, 66
- binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
- binding flavin mononucleotide: 62, 63, 172
- binding pyrrole-2-carboxylate: 65, 67, 69, 402
3ad7B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with methylthio acetate (see paper)
23% identity, 51% coverage: 192:411/429 of query aligns to 175:394/404 of 3ad7B
- active site: G326 (= G343), K358 (≠ M374)
- binding flavin-adenine dinucleotide: V196 (≠ A213), G225 (= G241), A226 (≠ D242), H228 (≠ L244), L247 (= L269), G353 (≠ S369), T354 (≠ G370), G355 (≠ Q371), G356 (= G372), F357 (≠ V373), K358 (≠ M374)
- binding flavin mononucleotide: V251 (≠ A273), K277 (≠ D298), E279 (≠ R300), R322 (≠ H339), W324 (= W341)
- binding [methylthio]acetate: M264 (≠ D285), Y271 (= Y292), T354 (≠ G370), K358 (≠ M374)
Sites not aligning to the query:
- active site: 62, 65, 66
- binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
- binding flavin mononucleotide: 62, 63, 172
- binding [methylthio]acetate: 67, 69, 402
1vrqB Crystal structure of heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with folinic acid (see paper)
23% identity, 51% coverage: 192:411/429 of query aligns to 175:394/402 of 1vrqB
- active site: G326 (= G343), K358 (≠ M374)
- binding n,n-dimethylglycine: K358 (≠ M374)
- binding flavin-adenine dinucleotide: V196 (≠ A213), A224 (≠ S240), G225 (= G241), H228 (≠ L244), L247 (= L269), G353 (≠ S369), T354 (≠ G370), G355 (≠ Q371), G356 (= G372), F357 (≠ V373), K358 (≠ M374)
- binding flavin mononucleotide: V251 (≠ A273), E279 (≠ R300), R322 (≠ H339), W324 (= W341)
Sites not aligning to the query:
- active site: 62, 65, 66
- binding n,n-dimethylglycine: 65, 67, 69, 401
- binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 51, 52, 53, 59, 60, 61, 63, 64, 65, 67
- binding flavin mononucleotide: 62, 63, 172
Query Sequence
>CCNA_03229 FitnessBrowser__Caulo:CCNA_03229
MTAQTPRPYPSPYGRGNWYADTVTDVPASPPLDGDVAAEVCIIGAGFTGLGAALALGSRA
VVLEAGRIGCAASGRNGGQVHTGQRRDQAWLEKTVGRDDALALWRLAEEARAHFASLVTA
IDCDWRPGMIHARHKPGGEAEDADYLALMADRYGYDQLELIGETALAHELGTDVYHGGLA
DHGGGHVHPLKLAQGMAAQARAAGAVIHENTRADAWRREGGKIVVETGGGRVTCDQLILS
GDGLLNRMAGPAQARVMPINNFIAVTAPLGALADEIIRSNAAVSDSRFVVNYFRKTPDGR
LLFGGGENYRPGFPHDIAGLVRKNLAKIYPRLADVEITHAWGGTLGITFSRMPFVGEVAQ
GVRVAAGYSGQGVMLAPYVGKLLAEAALGETGPVDLLSRLPTPPFPGGRLLRWPLTVAGL
SWYALRDRL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory