SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CCNA_03238 FitnessBrowser__Caulo:CCNA_03238 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
23% identity, 83% coverage: 53:305/306 of query aligns to 256:512/514 of P02916

query
sites
P02916

A

T

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V

S

T

G

T

T

G

F

W

H

K

N

N

Y

F

A

T

R

R

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C

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L

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L

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L

L

L

L

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P

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L

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L334 (= L132) mutation to W: Ability to transport lactose in a saturable manner.
L372 (≠ V176) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ L180) mutation N->K,H: No growth on maltose.
G380 (≠ H184) mutation G->C,S: No growth on maltose.
E401 (= E205) mutation E->A,C,K,L: Reduction of transport rate.
S403 (= S207) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G211) mutation G->A,P: No effect.
P420 (= P224) mutation to A: No effect.

Query Sequence

>CCNA_03238 FitnessBrowser__Caulo:CCNA_03238
MENLMEQSWKQAKAVFAAALGPPLVWLVLFFLAPMAIVWAYSFGHNVGLTEIAISGTFHN
YARAIEPLYLKIFLKSVWVAGLTTGLCLVIGFPVALAITFASDKAKAWLLLLIMLPFWTN
LLIRTYALIAVLRTEGYVNQGLEWFWKGGSGLATLIGLQPLGDFQPLELLHNNFAVILGL
VYVHLPFMVLPLYSALDRLDKSLLEASLDLGAGHLRTLFKVVVPLAIPGIASGVLITFIP
ALGAYLTPDLLGGPDSQMIANIIERQFKRANDWPFGAALSFLLMYLTFIAIAIQAVLNRK
APEAKG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory