SitesBLAST
Comparing CCNA_03360 FitnessBrowser__Caulo:CCNA_03360 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
A8B2U2 Fructose-bisphosphate aldolase; Glfba; glFBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 from Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia) (see 4 papers)
48% identity, 93% coverage: 1:336/363 of query aligns to 1:314/323 of A8B2U2
- S50 (= S50) binding
- D83 (= D83) active site, Proton donor; mutation to A: Severe loss of catalytic activity.
- H84 (= H84) binding
- H178 (= H198) binding ; binding
- G179 (= G199) binding
- K182 (= K202) binding
- H210 (= H232) binding
- G211 (= G233) binding
- S213 (= S235) binding
- N253 (= N275) binding
- D255 (= D277) binding ; mutation to A: 9.4-fold reduction in substrate affinity and 50-fold reduction in catalytic affinity. Has some activity towards tagatose-1,6-bisphosphate.
- S256 (≠ T278) binding
- R259 (= R281) binding ; mutation to A: 1.8-fold reduction in substrate affinity and 2.8-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-278.
- D278 (= D300) mutation to A: 159-fold reduction in substrate affinity and 2770-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-259.
- R280 (= R302) binding
3gayA Structure of giardia fructose-1,6-biphosphate aldolase in complex with tagatose-1,6-biphosphate (see paper)
48% identity, 91% coverage: 5:336/363 of query aligns to 4:310/319 of 3gayA
- binding 1,6-di-O-phosphono-D-tagatose: N23 (= N24), Q47 (= Q48), S49 (= S50), D82 (= D83), H83 (= H84), H174 (= H198), G175 (= G199), K178 (= K202), H206 (= H232), G207 (= G233), S208 (= S234), S209 (= S235), N249 (= N275), V250 (= V276), D251 (= D277), S252 (≠ T278), R255 (= R281)
- binding zinc ion: H83 (= H84), H174 (= H198), H206 (= H232)
3ohiA Structure of giardia fructose-1,6-biphosphate aldolase in complex with 3-hydroxy-2-pyridone (see paper)
48% identity, 91% coverage: 5:336/363 of query aligns to 4:310/319 of 3ohiA
- binding ({3-hydroxy-2-oxo-4-[2-(phosphonooxy)ethyl]pyridin-1(2H)-yl}methyl)phosphonic acid: N23 (= N24), S49 (= S50), D82 (= D83), H83 (= H84), H174 (= H198), G175 (= G199), K178 (= K202), H206 (= H232), G207 (= G233), S208 (= S234), S209 (= S235), N249 (= N275), V250 (= V276), D251 (= D277), S252 (≠ T278), R255 (= R281)
- binding zinc ion: H83 (= H84), H174 (= H198), H206 (= H232)
3gb6A Structure of giardia fructose-1,6-biphosphate aldolase d83a mutant in complex with fructose-1,6-bisphosphate (see paper)
48% identity, 91% coverage: 5:336/363 of query aligns to 4:309/318 of 3gb6A
- binding 1,6-di-O-phosphono-D-fructose: N23 (= N24), Q47 (= Q48), S49 (= S50), H173 (= H198), G174 (= G199), K177 (= K202), H205 (= H232), G206 (= G233), S207 (= S234), S208 (= S235), N248 (= N275), V249 (= V276), D250 (= D277), S251 (≠ T278), R254 (= R281)
2isvB Structure of giardia fructose-1,6-biphosphate aldolase in complex with phosphoglycolohydroxamate (see paper)
47% identity, 91% coverage: 5:336/363 of query aligns to 4:299/307 of 2isvB
- binding phosphoglycolohydroxamic acid: N23 (= N24), Q47 (= Q48), D82 (= D83), H83 (= H84), H168 (= H198), G169 (= G199), K172 (= K202), H195 (= H232), G196 (= G233), S197 (= S234), S198 (= S235), N238 (= N275), V239 (= V276), D240 (= D277), S241 (≠ T278)
- binding zinc ion: H83 (= H84), H168 (= H198), H195 (= H232)
2isvA Structure of giardia fructose-1,6-biphosphate aldolase in complex with phosphoglycolohydroxamate (see paper)
45% identity, 91% coverage: 5:336/363 of query aligns to 4:290/298 of 2isvA
- binding phosphoglycolohydroxamic acid: N23 (= N24), Q47 (= Q48), D82 (= D83), H83 (= H84), H163 (= H198), G164 (= G199), H186 (= H232), G187 (= G233), S188 (= S234), S189 (= S235), N229 (= N275), V230 (= V276), D231 (= D277), S232 (≠ T278)
- binding zinc ion: H83 (= H84), H163 (= H198), H186 (= H232)
1rv8B Class ii fructose-1,6-bisphosphate aldolase from thermus aquaticus in complex with cobalt (see paper)
41% identity, 90% coverage: 4:329/363 of query aligns to 3:305/305 of 1rv8B
- active site: D80 (= D83), H81 (= H84), E140 (= E150), H178 (= H198), H208 (= H232), N251 (= N275)
- binding cobalt (ii) ion: H81 (= H84), E132 (= E142), H178 (= H198), H208 (= H232)
- binding sulfate ion: S49 (= S50), G51 (= G52), R116 (= R126), H123 (= H133), G166 (= G186), G209 (= G233), S211 (= S235), N251 (= N275), T252 (≠ V276), D253 (= D277), T254 (= T278), R257 (= R281)
3n9sA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)- glycolohydroxamic acid bis- phosphate, a competitive inhibitor (see paper)
40% identity, 88% coverage: 8:326/363 of query aligns to 7:304/307 of 3n9sA
- active site: C69 (≠ V70), E70 (≠ D71), G136 (= G144), H180 (= H198), A226 (≠ Y248), N253 (= N275)
- binding calcium ion: D104 (= D105), S106 (= S107), E134 (= E142)
- binding 4-{hydroxy[(phosphonooxy)acetyl]amino}butyl dihydrogen phosphate: N23 (= N24), S49 (= S50), D82 (= D83), H83 (= H84), H180 (= H198), G181 (= G199), K184 (= K202), H210 (= H232), G211 (= G233), A212 (≠ S234), S213 (= S235), N253 (= N275), T254 (≠ V276), D255 (= D277), T256 (= T278)
- binding zinc ion: H83 (= H84), H180 (= H198), H210 (= H232)
3n9rA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)-phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
40% identity, 88% coverage: 8:326/363 of query aligns to 7:294/297 of 3n9rA
- active site: C69 (≠ V70), E70 (≠ D71), G136 (= G144), H170 (= H198), A216 (≠ Y248), N243 (= N275)
- binding 2-[hydroxy(4-hydroxybutyl)amino]-2-oxoethyl dihydrogen phosphate: D82 (= D83), H83 (= H84), H170 (= H198), G171 (= G199), K174 (= K202), H200 (= H232), G201 (= G233), A202 (≠ S234), S203 (= S235), N243 (= N275), T244 (≠ V276), D245 (= D277), T246 (= T278)
- binding zinc ion: H83 (= H84), H170 (= H198), H200 (= H232)
3c56A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(3-hydroxypropyl)-glycolohydroxamic acid bisphosphate, a competitive inhibitor (see paper)
40% identity, 88% coverage: 8:326/363 of query aligns to 7:294/297 of 3c56A
- active site: C69 (≠ V70), E70 (≠ D71), G136 (= G144), H170 (= H198), A216 (≠ Y248), N243 (= N275)
- binding 3-{hydroxy[(phosphonooxy)acetyl]amino}propyl dihydrogen phosphate: N23 (= N24), S49 (= S50), D82 (= D83), H83 (= H84), H170 (= H198), G171 (= G199), K174 (= K202), H200 (= H232), G201 (= G233), A202 (≠ S234), S203 (= S235), N243 (= N275), T244 (≠ V276), D245 (= D277), T246 (= T278), R249 (= R281)
- binding zinc ion: H83 (= H84), H170 (= H198), H200 (= H232)
3c52A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
39% identity, 88% coverage: 8:326/363 of query aligns to 7:293/296 of 3c52A
- active site: C69 (≠ V70), E70 (≠ D71), G136 (= G144), H169 (= H198), A215 (≠ Y248), N242 (= N275)
- binding calcium ion: D104 (= D105), S106 (= S107), E134 (= E142)
- binding phosphoglycolohydroxamic acid: Q47 (= Q48), D82 (= D83), H83 (= H84), H169 (= H198), G170 (= G199), K173 (= K202), H199 (= H232), G200 (= G233), A201 (≠ S234), S202 (= S235), N242 (= N275), T243 (≠ V276), D244 (= D277), T245 (= T278)
- binding zinc ion: H83 (= H84), H169 (= H198), H199 (= H232)
5ucpA Class ii fructose-1,6-bisphosphate aldolase e142a variant of helicobacter pylori with fbp and cleavage products (see paper)
39% identity, 88% coverage: 8:326/363 of query aligns to 7:289/292 of 5ucpA
- binding 1,6-di-O-phosphono-D-fructose: N23 (= N24), Q47 (= Q48), S49 (= S50), D82 (= D83), H83 (= H84), H165 (= H198), G166 (= G199), K169 (= K202), H195 (= H232), G196 (= G233), A197 (≠ S234), S198 (= S235), N238 (= N275), T239 (≠ V276), D240 (= D277), T241 (= T278), R244 (= R281)
- binding zinc ion: H83 (= H84), H83 (= H84), H83 (= H84), E134 (= E142), H165 (= H198), H165 (= H198), H165 (= H198), H195 (= H232), H195 (= H232)
5uckA Class ii fructose-1,6-bisphosphate aldolase of helicobacter pylori with cleavage products (see paper)
39% identity, 88% coverage: 8:326/363 of query aligns to 7:288/291 of 5uckA
- binding glyceraldehyde-3-phosphate: S49 (= S50), D82 (= D83), H83 (= H84), H164 (= H198), D239 (= D277), R243 (= R281)
- binding zinc ion: H83 (= H84), H83 (= H84), E134 (= E142), H164 (= H198), H194 (= H232), H194 (= H232)
5ud4A Class ii fructose-1,6-bisphosphate aldolase h180q variant of helicobacter pylori with tbp (see paper)
39% identity, 88% coverage: 8:326/363 of query aligns to 7:290/293 of 5ud4A