SitesBLAST

Comparing CCNA_03372 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 1 hits to proteins with known functional sites (download)

Q9HY80 Bacterioferritin-associated ferredoxin; Bfd from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
34% identity, 97% coverage: 1:59/61 of query aligns to 1:59/73 of Q9HY80

query
sites
Q9HY80

M

M

Y
|
Y

V

V

C
|
C

N
x
L

C
|
C

N

Q

G

G

I

V

R

T

E

D

R

N

E

Q

V

I

R

R

A

D

A

A

I

I

D

Y

A

E

G

G

A

C

T

C

R

S

P

Y

A
x
R

D

E

I

V

F
x
R

R

E

H

A

K

T

G

G

C

V

Q

G

A

T

Q

Q

C
|
C

A

G

K
|
K

C
|
C

V

A

C
|
C

E

L

M

A

R
x
K

Q

Q

M

V

I

V

Q

R

E

E

S

T

R

L

E

N

A

D

L

L

A
x
Q

Y
x
S

A
|
A

Y2 (= Y2) mutation to A: Does not bind [2Fe-2S] cluster.; mutation to F: Wild-type iron release from BfrB, 3-fold decreased affinity for BfrB.

C4 (= C4) binding [2Fe-2S] cluster

L5 (≠ N5) mutation to A: 2-fold decreased iron release from BfrB, 25-fold decreased affinity for BfrB.

C6 (= C6) binding [2Fe-2S] cluster

R26 (≠ A26) binding phosphate; mutation to E: No significant change in structure, [2Fe-2S] cluster or iron mobilization from BfrB, in truncated protein. No significant change in structure, [2Fe-2S] cluster or iron mobilization from BfrB, in truncated protein; when associated with Y-46.

R29 (≠ F29) binding phosphate; mutation to E: Probably unstable, it cannot be overexpressed, in truncated protein.

C38 (= C38) binding [2Fe-2S] cluster

K40 (= K40) mutation to A: Wild-type iron release from BfrB, 2-fold decreased affinity for BfrB.

C41 (= C41) binding [2Fe-2S] cluster

C43 (= C43) mutation to S: Increases yield of protein and stability of [2Fe-2S] center, no effect on its function.

K46 (≠ R46) binding phosphate; mutation to E: Probably unstable, it cannot be overexpressed, in truncated protein.; mutation to Y: No significant change in structure, [2Fe-2S] cluster or iron mobilization from BfrB, in truncated protein; when associated with E-26.

Sites not aligning to the query:

57:73 mutation Missing: No change in ability to mobilize iron from BfrB.

Query Sequence

>CCNA_03372
MYVCNCNGIREREVRAAIDAGATRPADIFRHKGCQAQCAKCVCEMRQMIQESREALAYAA
E

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory

SitesBLAST – Find functional sites

SitesBLAST

Query Sequence

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