SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CSW01_03560 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8a3lT 50S ribosomal protein L36 (see paper)
71% identity, 99% coverage: 2:86/86 of query aligns to 1:85/86 of 8a3lT

query
sites
8a3lT

A
|
A

N
|
N

N
x
I

K

K

S
|
S

A
|
A

K

K

K
|
K

R
|
R

A

A

I

I

Q
|
Q

A
x
S

E

E

K

K

R

A

R
|
R

Q

K

H
|
H

N
|
N

A

A

S
|
S

R
|
R

R
|
R

S
|
S

M
|
M

M

M

R
|
R

T
|
T

Y

F

M

I

K
|
K

K
|
K

T

V

V
x
Y

A

A

A

A

I

I

A

E

A

A

G

G

D

D

K

K

E

A

A

A

A

A

T

Q

A

K

A

A

F

F

A

N

V

E

V

M

T
x
Q

P
|
P

I

I

L

V

D
|
D

R
|
R

M

Q

A

A

T
x
A

K
|
K

G

G

L

L

I

I

H
|
H

K
|
K

N
|
N

K
|
K

A

A

A
|
A

R
|
R

H

H

K
|
K

S

A

R

N

F

L

F

T

A

A

A

Q

I

I

N

N

A

K

L

L

binding : A1 (= A2), N2 (= N3), I3 (≠ N4), S5 (= S6), A6 (= A7), K8 (= K9), R9 (= R10), Q12 (= Q13), S13 (≠ A14), R17 (= R18), H19 (= H20), N20 (= N21), S22 (= S23), R23 (= R24), R24 (= R25), S25 (= S26), M26 (= M27), R28 (= R29), T29 (= T30), K32 (= K33), K33 (= K34), Y35 (≠ V36), Q54 (≠ T55), P55 (= P56), D58 (= D59), R59 (= R60), A62 (≠ T63), K63 (= K64), H67 (= H68), K68 (= K69), N69 (= N70), K70 (= K71), A72 (= A73), R73 (= R74), K75 (= K76)

5wdtt 30S ribosomal protein S20 (see paper)
70% identity, 98% coverage: 3:86/86 of query aligns to 1:84/85 of 5wdtt

query
sites
5wdtt

N
|
N

N

I

K
|
K

S

S

A

A

K

K

K
|
K

R
|
R

A

A

I

I

Q

Q

A

S

E

E

K

K

R

A

R
|
R

Q

K

H
|
H

N
|
N

A

A

S
|
S

R
|
R

R
|
R

S
|
S

M
|
M

M

M

R
|
R

T
|
T

Y
x
F

M

I

K
|
K

K

K

T

V

V
x
Y

A

A

A

A

I

I

A

E

A

A

G

G

D

D

K

K

E

A

A

A

A

A

T

Q

A

K

A

A

F

F

A

N

V

E

V

M

T
x
Q

P
|
P

I

I

L

V

D
|
D

R
|
R

M

Q

A

A

T

A

K

K

G

G

L

L

I

I

H
|
H

K
|
K

N
|
N

K
|
K

A

A

A
|
A

R
|
R

H
|
H

K
|
K

S

A

R

N

F

L

F

T

A

A

A
x
Q

I

I

N

N

A

K

L

L

binding magnesium ion: S24 (= S26), R27 (= R29)
binding : N1 (= N3), K3 (= K5), K7 (= K9), R8 (= R10), R16 (= R18), H18 (= H20), N19 (= N21), S21 (= S23), R22 (= R24), R23 (= R25), S24 (= S26), M25 (= M27), R27 (= R29), T28 (= T30), F29 (≠ Y31), K31 (= K33), Y34 (≠ V36), Q53 (≠ T55), P54 (= P56), D57 (= D59), R58 (= R60), H66 (= H68), K67 (= K69), N68 (= N70), K69 (= K71), A71 (= A73), R72 (= R74), H73 (= H75), K74 (= K76), Q80 (≠ A82)

7unrt 30S ribosomal protein S6
67% identity, 99% coverage: 2:86/86 of query aligns to 1:85/87 of 7unrt

query
sites
7unrt

A
|
A

N
|
N

N

T

K

P

S
|
S

A

A

K

K

K
|
K

R
|
R

A

A

I

K

Q
|
Q

A

A

E

E

K

K

R
|
R

R
|
R

Q

S

H

H

N
|
N

A

A

S
|
S

R

L

R

R

S
|
S

M
|
M

M

V

R
|
R

T
|
T

Y

Y

M

I

K
|
K

K

N

T

V

V

V

A

K

A

A

I

I

A

D

A

A

G

K

D

D

K

L

E

E

A

K

A

A

T

Q

A

A

A

A

F

F

A

T

V

A

V

A

T

V

P

P

I

V

L

I

D
|
D

R
|
R

M

M

A

A

T
x
D

K
|
K

G

G

L

I

I

I

H
|
H

K

K

N
|
N

K
|
K

A

A

A

A

R
|
R

H

H

K
|
K

S
|
S

R
|
R

F

L

F
x
S

A

G

A

H

I

I

N

K

A

A

L

L

binding : A1 (= A2), N2 (= N3), S5 (= S6), K8 (= K9), R9 (= R10), Q12 (= Q13), R16 (= R17), R17 (= R18), N20 (= N21), S22 (= S23), S25 (= S26), M26 (= M27), R28 (= R29), T29 (= T30), K32 (= K33), D58 (= D59), R59 (= R60), D62 (≠ T63), K63 (= K64), H67 (= H68), N69 (= N70), K70 (= K71), R73 (= R74), K75 (= K76), S76 (= S77), R77 (= R78), S79 (≠ F80)

7m4ut A. Baumannii ribosome-eravacycline complex: 30s (see paper)
61% identity, 99% coverage: 2:86/86 of query aligns to 1:85/86 of 7m4ut

query
sites
7m4ut

A
|
A

N
|
N

N

S

K

A

S
x
Q

A

A

K

K

K
|
K

R
|
R

A

A

I
x
R

Q
|
Q

A
x
N

E

V

K

K

R
x
A

R
|
R

Q

K

H
|
H

N
|
N

A

A

S
|
S

R

L

R

R

S
|
S

M
|
M

M

V

R

R

T
|
T

Y

Y

M

I

K

K

K

R

T

T

V

L

A

S

A

A

I

I

A

A

A

G

G

G

D

D

K
x
Y

E

A

A

V

A

A

T

T

A

E

A

A

F

Y

A

K

V

K

V

A

T

V

P
|
P

I

V

L

I

D
|
D

R
|
R

M

M

A

A

T
x
D

K
|
K

G

G

L

I

I

I

H
|
H

K

K

N
|
N

K
|
K

A

A

A
|
A

R
|
R

H

H

K
|
K

S
|
S

R
|
R

F

L

F
x
N

A

A

A
x
Q

I

V

N
x
K

A

A

L

L

binding : A1 (= A2), N2 (= N3), Q5 (≠ S6), K8 (= K9), R9 (= R10), R11 (≠ I12), Q12 (= Q13), N13 (≠ A14), A16 (≠ R17), R17 (= R18), H19 (= H20), N20 (= N21), S22 (= S23), S25 (= S26), M26 (= M27), T29 (= T30), Y43 (≠ K44), P55 (= P56), D58 (= D59), R59 (= R60), D62 (≠ T63), K63 (= K64), H67 (= H68), N69 (= N70), K70 (= K71), A72 (= A73), R73 (= R74), K75 (= K76), S76 (= S77), R77 (= R78), N79 (≠ F80), Q81 (≠ A82), K83 (≠ N84)

8rdvTo Small ribosomal subunit protein bS20 (see paper)
60% identity, 99% coverage: 2:86/86 of query aligns to 1:85/87 of 8rdvTo

query
sites
8rdvTo

A
|
A

N
|
N

N

T

K

A

S
x
Q

A

A

K

R

K
|
K

R
|
R

A

A

I
x
R

Q
|
Q

A
x
N

E

T

K

K

R
|
R

R
|
R

Q

Q

H

N

N

S

A

A

S
|
S

R
x
Q

R
|
R

S
|
S

M
|
M

M

V

R
|
R

T
|
T

Y

Y

M

L

K
|
K

K
x
R

T

V

V

D

A

A

A

A

I

I

A

A

A

A

G

K

D

D

K
x
Y

E

D

A

A

A

A

T

T

A

E

A

A

F

Y

A

K

V

K

V

A

T

I

P
|
P

I

V

L

L

D
|
D

R
|
R

M

M

A

A

T
x
D

K
|
K

G

G

L

I

I

I

H
|
H

K

K

N
|
N

K
|
K

A

A

A
|
A

R
|
R

H
x
R

K
|
K

S
|
S

R
|
R

F

L

F
x
N

A

K

A

T

I

I

N
x
K

A

G

L

L

binding : A1 (= A2), N2 (= N3), Q5 (≠ S6), K8 (= K9), R9 (= R10), R11 (≠ I12), Q12 (= Q13), N13 (≠ A14), R16 (= R17), R17 (= R18), S22 (= S23), Q23 (≠ R24), R24 (= R25), S25 (= S26), M26 (= M27), R28 (= R29), T29 (= T30), K32 (= K33), R33 (≠ K34), Y43 (≠ K44), P55 (= P56), D58 (= D59), R59 (= R60), D62 (≠ T63), K63 (= K64), H67 (= H68), N69 (= N70), K70 (= K71), A72 (= A73), R73 (= R74), R74 (≠ H75), K75 (= K76), S76 (= S77), R77 (= R78), N79 (≠ F80), K83 (≠ N84)

8crxT 30S ribosomal protein S17 (see paper)
46% identity, 98% coverage: 3:86/86 of query aligns to 2:85/87 of 8crxT

query
sites
8crxT

N
|
N

N

I

K

K

S
|
S

A
x
Q

K

I

K
|
K

R
|
R

A

V

I
x
K

Q

T

A
x
N

E

E

K

K

R
x
S

R
|
R

Q

Q

H
x
R

N
|
N

A
x
K

S
x
A

R

V

R

K

S
|
S

M
x
A

M

L

R
|
R

T
|
T

Y

Y

M

V

K

R

K

N

T

F

V

R

A

R

A

A

I

A

A

E

A

A

G

G

D

D

K

V

E

E

A

A

A

A

T

T

A

K

A

A

F

A

A
x
R

V

V

V

A

T

N

P
x
R

I

Q

L

L

D
|
D

R
x
K

M

A

A

A

T
x
S

K
|
K

G

G

L

V

I

I

H
|
H

K

K

N
|
N

K
x
Q

A

A

A
|
A

R
x
N

H

R

K
|
K

S
|
S

R

A

F

I

F
x
S

A

K

A

K

I

L

N
|
N

A

S

L

L

binding : N2 (= N3), S5 (= S6), Q6 (≠ A7), K8 (= K9), R9 (= R10), K11 (≠ I12), N13 (≠ A14), S16 (≠ R17), R17 (= R18), R19 (≠ H20), N20 (= N21), K21 (≠ A22), A22 (≠ S23), S25 (= S26), A26 (≠ M27), R28 (= R29), T29 (= T30), R51 (≠ A52), R55 (≠ P56), D58 (= D59), K59 (≠ R60), S62 (≠ T63), K63 (= K64), H67 (= H68), N69 (= N70), Q70 (≠ K71), A72 (= A73), N73 (≠ R74), K75 (= K76), S76 (= S77), S79 (≠ F80), N83 (= N84)

Sites not aligning to the query:

binding : 1

8buut ExpZ (see paper)
40% identity, 98% coverage: 3:86/86 of query aligns to 2:85/86 of 8buut

query
sites
8buut

N
|
N

N
x
I

K
|
K

S
|
S

A

A

K

I

K
|
K

R
|
R

A
x
T

I
x
K

Q

T

A
x
N

E

N

K

E

R
|
R

R
|
R

Q

V

H
|
H

N
|
N

A

A

S
x
T

R

I

R

K

S
|
S

M
x
A

M

M

R
|
R

T
|
T

Y

A

M

I

K
|
K

K

Q

T

V

V

E

A

A

A

S

I

V

A

A

A

N

G

N

D

E

K

A

E

D

A

K

A

A

T

K

A

T

A

A

F

L

A
x
T

V

E

V

A

T
x
A

P
x
K

I

R

L

I

D
|
D

R
x
K

M

A

A

V

T
x
K

K

T

G

G

L

L

I

V

H
|
H

K

K

N
|
N

K
x
T

A

A

A
|
A

R
|
R

H

Y

K
|
K

S
|
S

R
|
R

F

L

F
x
A

A

K

A

K

I

V

N
|
N

A

G

L

L

binding : N2 (= N3), I3 (≠ N4), K4 (= K5), S5 (= S6), K8 (= K9), R9 (= R10), T10 (≠ A11), K11 (≠ I12), N13 (≠ A14), R16 (= R17), R17 (= R18), H19 (= H20), N20 (= N21), T22 (≠ S23), S25 (= S26), A26 (≠ M27), R28 (= R29), T29 (= T30), K32 (= K33), T51 (≠ A52), A54 (≠ T55), K55 (≠ P56), D58 (= D59), K59 (≠ R60), K62 (≠ T63), H67 (= H68), N69 (= N70), T70 (≠ K71), A72 (= A73), R73 (= R74), K75 (= K76), S76 (= S77), R77 (= R78), A79 (≠ F80), N83 (= N84)

Sites not aligning to the query:

binding : 1

8fmwT 30S ribosomal protein S10 (see paper)
46% identity, 92% coverage: 1:79/86 of query aligns to 1:79/85 of 8fmwT

query
sites
8fmwT

M
|
M

A
x
R

N

K

N
|
N

K

A

S

S

A

A

K

L

K
|
K

R
|
R

A

S

I
x
R

Q
|
Q

A
x
N

E

L

K

K

R
|
R

R
x
K

Q

I

H
x
R

N
|
N

A

V

S

S

R

V

R

K

S
|
S

M
x
E

M

L

R
x
K

T
|
T

Y

I

M

E

K
|
K

K
x
R

T

C

V

I

A

N

A

M

I

I

A

K

A

A

G

G

D

K

K

K

E

D

A

E

A

A

T

I

A

E

A

F

F

F

A
x
K

V

F

V

V

T

A

P
x
K

I

K

L

L

D
|
D

R

T

M

A

A

A

T
x
R

K
|
K

G
x
R

L

I

I

I

H
|
H

K

K

N
|
N

K
|
K

A

A

A
|
A

R
|
R

H

K

K
|
K

S
|
S

R
|
R

F

L

binding : M1 (= M1), R2 (≠ A2), N4 (= N4), K9 (= K9), R10 (= R10), R12 (≠ I12), Q13 (= Q13), N14 (≠ A14), R17 (= R17), K18 (≠ R18), R20 (≠ H20), N21 (= N21), S26 (= S26), E27 (≠ M27), K29 (≠ R29), T30 (= T30), K33 (= K33), R34 (≠ K34), K52 (≠ A52), K56 (≠ P56), D59 (= D59), R63 (≠ T63), K64 (= K64), R65 (≠ G65), H68 (= H68), N70 (= N70), K71 (= K71), A73 (= A73), R74 (= R74), K76 (= K76), S77 (= S77), R78 (= R78)

Sites not aligning to the query:

binding : 80, 84

6dzi7 50S ribosomal protein L29 (see paper)
41% identity, 99% coverage: 2:86/86 of query aligns to 1:85/85 of 6dzi7

query
sites
6dzi7

A
|
A

N
|
N

N
x
I

K
|
K

S
|
S

A
x
Q

K

I

K
|
K

R
|
R

A
x
I

I
x
R

Q

T

A
x
N

E

E

K

R

R
|
R

R
|
R

Q

L

H

R

N
|
N

A

Q

S
|
S

R

V

R

K

S
|
S

M
x
S

M

L

R
|
R

T
|
T

Y

A

M

I

K
x
R

K

G

T

F

V

R

A

E

A

A

I

V

A

D

A

A

G

G

D

D

K

K

E

D

A

K

A

A

T

S

A

E

A

L

F

L

A
x
H

V

A

V

T

T

S

P
x
R

I

K

L

L

D
|
D

R
x
K

M

A

A

A

T
x
S

K
|
K

G

G

L

V

I

I

H
|
H

K

P

N
|
N

K
x
Q

A

A

A

A

R

N

H
x
K

K
|
K

S

S

R

A

F

L

F

A

A

L

A

A

I

L

N
|
N

A

K

L

L

binding : A1 (= A2), N2 (= N3), I3 (≠ N4), K4 (= K5), S5 (= S6), Q6 (≠ A7), K8 (= K9), R9 (= R10), I10 (≠ A11), R11 (≠ I12), N13 (≠ A14), R16 (= R17), R17 (= R18), N20 (= N21), S22 (= S23), S25 (= S26), S26 (≠ M27), R28 (= R29), T29 (= T30), R32 (≠ K33), H51 (≠ A52), R55 (≠ P56), D58 (= D59), K59 (≠ R60), S62 (≠ T63), K63 (= K64), H67 (= H68), N69 (= N70), Q70 (≠ K71), K74 (≠ H75), K75 (= K76), N83 (= N84)

8uu5t Small ribosomal subunit protein uS9 (see paper)
47% identity, 94% coverage: 3:83/86 of query aligns to 2:81/81 of 8uu5t

query
sites
8uu5t

N
|
N

N
x
I

K

K

S
|
S

A

A

K

I

K
|
K

R
|
R

A

V

I

K

Q

T

A

A

E

E

K

T

R
|
R

R
x
N

Q

S

H
x
R

N
|
N

A

A

S

S

R

Q

R

R

S
|
S

M
x
A

M

M

R

R

T
|
T

Y

A

M

I

K

K

K

K

T

-

V

F

A

D

A

E

I

A

A

A

A

A

G

N

D

N

K

A

E

D

A

N

A

A

T

K

A

D

A

L

F

Y

A

V

V

E

V

A

T

S

P
x
K

I

K

L

L

D
|
D

R

S

M

A

A

V

T

S

K
|
K

G

G

L

L

I

I

H
|
H

K
|
K

N
|
N

K
x
N

A

A

A
|
A

R
|
R

H

N

K
|
K

S

S

R
|
R

F

L

F

A

A

A

A

K

I

L

binding magnesium ion: H66 (= H68), N68 (= N70)
binding : N2 (= N3), I3 (≠ N4), S5 (= S6), K8 (= K9), R9 (= R10), R16 (= R17), N17 (≠ R18), R19 (≠ H20), N20 (= N21), S25 (= S26), A26 (≠ M27), T29 (= T30), K54 (≠ P56), D57 (= D59), K62 (= K64), K67 (= K69), N69 (≠ K71), A71 (= A73), R72 (= R74), K74 (= K76), R76 (= R78)

Sites not aligning to the query:

binding : 1

8uu4t Small ribosomal subunit protein uS8 (see paper)
47% identity, 94% coverage: 3:83/86 of query aligns to 2:81/82 of 8uu4t

query
sites
8uu4t

N
|
N

N
x
I

K
|
K

S
|
S

A

A

K

I

K
|
K

R
|
R

A

V

I
x
K

Q

T

A

A

E

E

K

T

R
|
R

R
x
N

Q

S

H

R

N
|
N

A

A

S

S

R

Q

R

R

S
|
S

M

A

M

M

R
|
R

T

T

Y

A

M

I

K
|
K

K

K

T

-

V

F

A

D

A

E

I

A

A

A

A

A

G

N

D

N

K

A

E

D

A

N

A

A

T

K

A

D

A

L

F

Y

A

V

V

E

V

A

T
x
S

P
x
K

I

K

L

L

D
|
D

R

S

M

A

A

V

T
x
S

K
|
K

G

G

L

L

I

I

H
|
H

K

K

N
|
N

K
x
N

A

A

A
|
A

R
|
R

H

N

K

K

S
|
S

R
|
R

F

L

F

A

A

A

A

K

I

L

binding : N2 (= N3), I3 (≠ N4), K4 (= K5), S5 (= S6), K8 (= K9), R9 (= R10), K11 (≠ I12), R16 (= R17), N17 (≠ R18), N20 (= N21), S25 (= S26), R28 (= R29), K32 (= K33), S53 (≠ T55), K54 (≠ P56), D57 (= D59), S61 (≠ T63), K62 (= K64), H66 (= H68), N68 (= N70), N69 (≠ K71), A71 (= A73), R72 (= R74), S75 (= S77), R76 (= R78)

Sites not aligning to the query:

binding : 1

7msct 70SIC in complex with MtbEttA at Pre_R0 state (see paper)
41% identity, 99% coverage: 2:86/86 of query aligns to 1:85/85 of 7msct

query
sites
7msct

A
|
A

N
|
N

N
x
I

K
|
K

S
|
S

A
x
Q

K

Q

K
|
K

R
|
R

A
x
N

I
x
R

Q

T

A
x
N

E

E

K

R

R
x
A

R
|
R

Q

L

H

R

N
|
N

A

K

S

A

R

V

R

K

S
|
S

M

S

M

L

R
|
R

T
|
T

Y

A

M

V

K
x
R

K

A

T

F

V

R

A

E

A

A

I

A

A

H

A

A

G

G

D

D

K

K

E

A

A

K

A

A

T

A

A

E

A

L

F

L

A

A

V

S

V

T

T

N

P
x
R

I

K

L

L

D
|
D

R
x
K

M

A

A

A

T
x
S

K

K

G

G

L

V

I

I

H
|
H

K
|
K

N
|
N

K

Q

A

A

A
|
A

R
x
N

H
x
K

K
|
K

S
|
S

R

A

F

L

F

A

A

Q

A

A

I

L

N
|
N

A

K

L

L

binding : A1 (= A2), N2 (= N3), I3 (≠ N4), K4 (= K5), S5 (= S6), Q6 (≠ A7), K8 (= K9), R9 (= R10), N10 (≠ A11), R11 (≠ I12), N13 (≠ A14), A16 (≠ R17), R17 (= R18), N20 (= N21), S25 (= S26), R28 (= R29), T29 (= T30), R32 (≠ K33), R55 (≠ P56), D58 (= D59), K59 (≠ R60), S62 (≠ T63), H67 (= H68), K68 (= K69), N69 (= N70), A72 (= A73), N73 (≠ R74), K74 (≠ H75), K75 (= K76), S76 (= S77), N83 (= N84)

5mmjt Structure of the small subunit of the chloroplast ribosome (see paper)
38% identity, 86% coverage: 6:79/86 of query aligns to 7:89/107 of 5mmjt

query
sites
5mmjt

S

S

A

A

K

A

K
|
K

R
|
R

A

T

I
x
R

Q
|
Q

A

A

E

E

K

T

R
|
R

R

R

Q

L

H

R

N
|
N

A
x
K

S

A

R

R

R

K

S
|
S

M
x
E

M

V

R
x
K

T
|
T

Y

R

M

M

K
x
R

K
|
K

T

V

V

F

A

E

A

A

I

L

A

D

A

A

G

L

D

K

K

K

E

K

A

S

A

G

T

A

A

S

A

T

F

E

A

E

V

L

V

V

T

-

P

P

I

I

-

D

-

N

-

L

-

I

-
x
A

-

E

-

A

-
x
Y

-
x
S

-

A

L

I

D
|
D

R
x
K

M

A

A

V

T

V

K
|
K

G

G

L

T

I

L

H
|
H

K
x
R

N
|
N

K
x
T

A

A

A

A

R
|
R

H

R

K
|
K

S
|
S

R
|
R

F

L

binding : K10 (= K9), R11 (= R10), R13 (≠ I12), Q14 (= Q13), R18 (= R17), N22 (= N21), K23 (≠ A22), S27 (= S26), E28 (≠ M27), K30 (≠ R29), T31 (= T30), R34 (≠ K33), K35 (= K34), A62 (vs. gap), Y65 (vs. gap), S66 (vs. gap), D69 (= D59), K70 (≠ R60), K74 (= K64), H78 (= H68), R79 (≠ K69), N80 (= N70), T81 (≠ K71), R84 (= R74), K86 (= K76), S87 (= S77), R88 (= R78)

Sites not aligning to the query:

binding : 6, 90, 93, 94, 103

4v61AT Ribosomal Protein S8 (see paper)
40% identity, 86% coverage: 6:79/86 of query aligns to 10:92/102 of 4v61AT

query
sites
4v61AT

S

S

A

A

K

A

K
|
K

R

R

A

A

I

R

Q
|
Q

A

A

E

E

K

K

R
|
R

R
|
R

Q

V

H

Y

N
|
N

A

K

S

S

R

K

R

K

S

S

M

E

M

A

R
|
R

T
|
T

Y
x
R

M

M

K
|
K

K

K

T

V

V

L

A

E

A

A

I

L

A

E

A

G

G

L

D

K

K

K

E

K

A

T

A

D

T

A

A

Q

A

A

F

D

A

E

V

I

V

V

T

T

-

V

-

E

-

K

-

L

-

I

-

G

-

E

-

A

-

Y

P

S

I

A

L

I

D
|
D

R

K

M

A

A

V

T
x
K

K

V

G
x
K

L

A

I

L

H

H

K
|
K

N
|
N

K
x
T

A

G

A
|
A

R
|
R

H

R

K

K

S
|
S

R
|
R

F

L

binding : K13 (= K9), Q17 (= Q13), R21 (= R17), R22 (= R18), N25 (= N21), R33 (= R29), T34 (= T30), R35 (≠ Y31), K37 (= K33), D72 (= D59), K76 (≠ T63), K78 (≠ G65), K82 (= K69), N83 (= N70), T84 (≠ K71), A86 (= A73), R87 (= R74), S90 (= S77), R91 (= R78)

Sites not aligning to the query:

binding : 95

6w6pt 30S ribosomal protein S20 (see paper)
42% identity, 90% coverage: 3:79/86 of query aligns to 2:77/82 of 6w6pt

query
sites
6w6pt

N
|
N

N
x
I

K

E

S

S

A

A

K

I

K

K

R
|
R

A

V

I
x
R

Q

T

A

S

E

A

K

N

R

A

R
x
N

Q

A

H

K

N
|
N

A

S

S

S

R

Q

R

T

S
x
N

M

A

M

M

R
|
R

T
|
T

Y

A

M

I

K

K

K
|
K

T

F

V

E

A

E

A

A

I

V

A

A

A

A

G

G

D

-

K

A

E

D

A

N

A

V

T

D

A

A

A

L

F

Y

A
x
N

V

E

V

A

T

V

P
x
K

I

A

L

V

D
|
D

R
x
M

M

A

A

A

T
|
T

K
|
K

G

G

L

L

I

I

H

H

K
|
K

N
|
N

K

K

A

A

A

N

R
|
R

H

D

K

K

S

I

R

R

F

L

binding : N2 (= N3), I3 (≠ N4), R9 (= R10), R11 (≠ I12), N17 (≠ R18), N20 (= N21), N25 (≠ S26), R28 (= R29), T29 (= T30), K33 (= K34), N50 (≠ A52), K54 (≠ P56), D57 (= D59), M58 (≠ R60), T61 (= T63), K62 (= K64), K67 (= K69), N68 (= N70), R72 (= R74)

Sites not aligning to the query:

binding : 1

7azsS20A 50S ribosomal protein L20 (see paper)
35% identity, 97% coverage: 4:86/86 of query aligns to 2:84/99 of 7azsS20A

query
sites
7azsS20A

N
|
N

K

L

S
|
S

A

A

K

L

K

K

R
|
R

A

H

I
x
R

Q
|
Q

A
x
S

E

L

K

K

R
|
R

R
|
R

Q

L

H
x
R

N
|
N

A
x
K

S
x
A

R

K

R

K

S
|
S

M
x
A

M

I

R
x
K

T
|
T

Y
x
L

M

S

K
|
K

K
|
K

T

A

V

I

A

Q

A

L

I

A

A

Q

A

E

G

G

D

K

K

A

E

E

A
x
E

A

A

T

L

A

K

A
x
I

F

M

A
x
R

V

K

V

A

T
x
E

P

S

I

L

L

I

D
|
D

R
x
K

M

A

A

A

T
x
K

K

G

G
x
S

L
x
T

I

L

H
|
H

K

K

N
|
N

K

A

A

A

A
|
A

R
|
R

H
x
R

K
|
K

S
|
S

R
|
R

F

L

F
x
M

A

R

A

K

I

V

N
x
R

A

Q

L

L

binding osmium (III) hexammine: S63 (≠ G65), T64 (≠ L66)
binding : N2 (= N4), S4 (= S6), R8 (= R10), R10 (≠ I12), Q11 (= Q13), S12 (≠ A14), R15 (= R17), R16 (= R18), R18 (≠ H20), N19 (= N21), K20 (≠ A22), A21 (≠ S23), S24 (= S26), A25 (≠ M27), K27 (≠ R29), T28 (= T30), L29 (≠ Y31), K31 (= K33), K32 (= K34), E44 (≠ A46), I48 (≠ A50), R50 (≠ A52), E53 (≠ T55), D57 (= D59), K58 (≠ R60), K61 (≠ T63), S63 (≠ G65), H66 (= H68), N68 (= N70), A71 (= A73), R72 (= R74), R73 (≠ H75), K74 (= K76), S75 (= S77), R76 (= R78), M78 (≠ F80), R82 (≠ N84)

Sites not aligning to the query:

binding : 1, 94, 96, 97, 98

1i94T Crystal structures of the small ribosomal subunit with tetracycline, edeine and if3 (see paper)
35% identity, 97% coverage: 4:86/86 of query aligns to 2:84/99 of 1i94T

query
sites
1i94T

N
|
N

K
x
L

S
|
S

A

A

K

L

K

K

R

R

A
x
H

I
x
R

Q
|
Q

A
x
S

E
x
L

K

K

R

R

R

R

Q

L

H

R

N

N

A

K

S
x
A

R

K

R

K

S

S

M

A

M

I

R

K

T

T

Y

L

M

S

K

K

K

K

T

A

V

I

A

Q

A

L

I

A

A

Q

A

E

G

G

D

K

K

A

E

E

A

E

A

A

T

L

A

K

A

I

F

M

A

R

V

K

V

A

T

E

P
x
S

I

L

L

I

D

D

R

K

M

A

A

A

T

K

K

G

G

S

L

T

I

L

H

H

K

K

N

N

K
x
A

A
|
A

A
|
A

R
|
R

H
x
R

K
|
K

S
|
S

R

R

F

L

F
x
M

A

R

A

K

I

V

N

R

A

Q

L

L

binding magnesium ion: N2 (= N4), L3 (≠ K5), S4 (= S6), H9 (≠ A11), R10 (≠ I12), Q11 (= Q13), S12 (≠ A14), L13 (≠ E15), A69 (≠ K71), A70 (= A72), A71 (= A73), R72 (= R74), R73 (≠ H75), K74 (= K76)
binding : A21 (≠ S23), S54 (≠ P56), S75 (= S77), M78 (≠ F80)

Sites not aligning to the query:

binding : 94, 95, 96, 98

4v8iCT structure of YfiA bound to the 70S ribosome. (see paper)
35% identity, 97% coverage: 4:86/86 of query aligns to 7:89/104 of 4v8iCT

query
sites
4v8iCT

N

N

K
x
L

S

S

A
|
A

K

L

K
|
K

R
|
R

A
x
H

I
x
R

Q
|
Q

A

S

E

L

K

K

R
|
R

R
|
R

Q

L

H

R

N
|
N

A

K

S
x
A

R
x
K

R

K

S
|
S

M

A

M

I

R
x
K

T
|
T

Y

L

M

S

K

K

K
|
K

T

A

V

I

A

Q

A

L

I

A

A

Q

A

E

G

G

D

K

K

A

E

E

A
x
E

A

A

T

L

A
x
K

A

I

F

M

A
x
R

V

K

V

A

T
x
E

P
x
S

I

L

L

I

D
|
D

R
x
K

M

A

A

A

T
x
K

K

G

G
x
S

L

T

I

L

H

H

K
|
K

N
|
N

K

A

A

A

A
|
A

R
|
R

H

R

K

K

S
|
S

R
|
R

F

L

F
x
M

A
x
R

A

K

I

V

N
x
R

A

Q

L

L

binding : L8 (≠ K5), A10 (= A7), K12 (= K9), R13 (= R10), H14 (≠ A11), R15 (≠ I12), Q16 (= Q13), R20 (= R17), R21 (= R18), N24 (= N21), A26 (≠ S23), K27 (≠ R24), S29 (= S26), K32 (≠ R29), T33 (= T30), K37 (= K34), E49 (≠ A46), K52 (≠ A49), R55 (≠ A52), E58 (≠ T55), S59 (≠ P56), D62 (= D59), K63 (≠ R60), K66 (≠ T63), S68 (≠ G65), K72 (= K69), N73 (= N70), A76 (= A73), R77 (= R74), S80 (= S77), R81 (= R78), M83 (≠ F80), R84 (≠ A81), R87 (≠ N84)

Sites not aligning to the query:

binding : 1, 2, 3, 99, 100, 101, 102

6fxcAt Ribosome hibernation promotion factor (see paper)
39% identity, 94% coverage: 2:82/86 of query aligns to 1:80/81 of 6fxcAt

query
sites
6fxcAt

A
|
A

N
|
N

N

I

K

K

S
|
S

A

A

K

I

K
|
K

R
|
R

A

V

I

K

Q

T

A
x
T

E

E

K
|
K

R

A

R
x
E

Q

A

H

R

N
|
N

A

I

S

S

R

Q

R

K

S
|
S

M

A

M

M

R
|
R

T
|
T

Y

A

M

V

K

K

K

N

T

A

V

K

A

T

A

A

I

V

A

S

A

N

G

N

-

A

D

D

K

N

E

K

A

N

A

E

T

L

A

V

A

S

F

L

A

A

V

V

V

-

T

-

P
x
K

I

L

L

V

D
|
D

R
x
K

M

A

A

A

T
x
Q

K

S

G
x
N

L

L

I

I

H
|
H

K

S

N
|
N

K
|
K

A

A

A
x
D

R
|
R

H

I

K

K

S
|
S

R

Q

F

L

F
x
M

A
x
T

A

A

binding : A1 (= A2), N2 (= N3), S5 (= S6), K8 (= K9), R9 (= R10), T13 (≠ A14), K15 (= K16), E17 (≠ R18), N20 (= N21), S25 (= S26), R28 (= R29), T29 (= T30), K54 (≠ P56), D57 (= D59), K58 (≠ R60), Q61 (≠ T63), N63 (≠ G65), H66 (= H68), N68 (= N70), K69 (= K71), D71 (≠ A73), R72 (= R74), S75 (= S77), M78 (≠ F80), T79 (≠ A81)

5myjAT of 70S ribosome from Lactococcus lactis (see paper)
37% identity, 76% coverage: 19:83/86 of query aligns to 13:70/71 of 5myjAT

query
sites
5myjAT

Q

E

H
x
R

N
|
N

A

A

S

Q

R

Q

R
x
K

S
|
S

M

A

M

M

R
|
R

T
|
T

Y

L

M

I

K
|
K

K
|
K

T

F

V

E

A

A

A

A

I

-

A

-

A

-

G

-

D

-

K

-

E

-

A

P

A

S

T
x
E

A

E

A

L

F

Y

A
x
R

V

A

V

A

T

S

P
x
S

I

T

L

I

D
|
D

R
x
K

M
x
A

A

A

T
x
S

K
|
K

G

G

L
|
L

I

I

H
|
H

K
x
A

N
|
N

K

K

A

A

A

S

R
|
R

H

D

K
|
K

S

A

R
|
R

F

L

F

A

A

A

A

K

I

L

binding : R14 (≠ H20), N15 (= N21), K19 (≠ R25), S20 (= S26), R23 (= R29), T24 (= T30), K27 (= K33), K28 (= K34), E35 (≠ T48), R39 (≠ A52), S43 (≠ P56), D46 (= D59), K47 (≠ R60), A48 (≠ M61), S50 (≠ T63), K51 (= K64), L53 (= L66), H55 (= H68), A56 (≠ K69), N57 (= N70), R61 (= R74), K63 (= K76), R65 (= R78)

Sites not aligning to the query:

binding : 1, 4, 7, 8, 12

Query Sequence

>CSW01_03560
MANNKSAKKRAIQAEKRRQHNASRRSMMRTYMKKTVAAIAAGDKEAATAAFAVVTPILDR
MATKGLIHKNKAARHKSRFFAAINAL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory