SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing DVU1367 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

Q9XH46 Sec-independent protein translocase protein TATA, chloroplastic; Protein THYLAKOID ASSEMBLY 4; Protein TWIN-ARGININE TRANSLOCATION A from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see 4 papers)
44% identity, 69% coverage: 1:45/65 of query aligns to 58:102/137 of Q9XH46

query
sites
Q9XH46

M

F

F

F

G
|
G

I

L

G
|
G

F

V

Q
x
P

E
|
E

L

L

L

V

V

V

V

I

L

A

V

G

L

V

V

A

L

A

L
|
L

V

V

F

F

G

G

A

P

N

K

K

K

L

L

P

P

E

E

I

V

G

G

G

R

G

S

L

I

G

G

R

Q

A

T

I

V

R

K

N

S

F

F

K

Q

R

Q

A

A

S

K

E

E

G60 (= G3) mutation to C: Loss of protein translocation; when associated with C-64.
G62 (= G5) mutation to C: Loss of protein translocation; when associated with C-64.
P64 (≠ Q7) mutation to C: Loss of protein translocation; when associated with C-60 or C-62.
E65 (= E8) mutation E->A,D,Q: Loss of protein translocation.
L75 (= L18) mutation to I: No effect on protein translocation.

P69428 Sec-independent protein translocase protein TatA from Escherichia coli (strain K12) (see paper)
46% identity, 85% coverage: 1:55/65 of query aligns to 1:56/89 of P69428

query
sites
P69428

M

M

F

G

G

G

I

I

G

S

F

I

Q

W

E

Q

L

L

V

I

L

A

V

V

L

I

V

V

L

V

L

L

V

L

F

F

G

G

A

T

N

K

K

K

L

L

P

G

E

S

I

I

G

G

G

S

G

D

L

L

G

G

R

A

A

S

I

I

R

K

N

G

F
|
F

K

K

R

K

A

A

A

M

S

S

-

D

E

E

P

P

D

K

E

Q

I

-

D

D

V

K

T

T

P

S

T

Q

D

D

F39 (= F39) mutation to A: Homooligomerizes more efficiently than wt, blocks translocation.

Q94G16 Sec-independent protein translocase protein TATB, chloroplastic; Protein HIGH CHLOROPHYLL FLUORESCENCE 106; Protein TWIN-ARGININE TRANSLOCATION B from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see paper)
39% identity, 75% coverage: 1:49/65 of query aligns to 89:137/261 of Q94G16

query
sites
Q94G16

M

L

F

F

G

G

I

V

G

G

F

A

Q

P

E
|
E

L

A

L

L

V

V

V

I

L

G

V

V

L

V

V

A

L

L

V

V

F

F

G

G

A

P

N

K

K

G

L

L

P

A

E

E

I

V

G

A

G

R

G

N

L

L

G

G

R

K

A

T

I

L

R

R

N

E

F

F

K

Q

R

P

A

T

A

I

S

R

E

E

P

I

D

Q

E

D

I

V

E96 (= E8) mutation to Q: Loss of protein translocation.

Query Sequence

>DVU1367
MFGIGFQELLVVLVLVLLVFGANKLPEIGGGLGRAIRNFKRAASEPDEIDVTPTDKKDKN
DDKQA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory