SitesBLAST
Comparing Dsui_0124 Dsui_0124 lactate dehydrogenase-like oxidoreductase to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
38% identity, 83% coverage: 53:320/322 of query aligns to 57:323/334 of 5aovA
- active site: L100 (≠ A96), R241 (= R232), D265 (= D256), E270 (= E261), H288 (= H285)
- binding glyoxylic acid: Y74 (≠ A70), A75 (= A71), V76 (≠ T72), G77 (= G73), R241 (= R232), H288 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ T72), T104 (≠ V100), F158 (≠ G153), G159 (= G154), R160 (≠ S155), I161 (≠ L156), S180 (vs. gap), R181 (vs. gap), A211 (≠ H202), V212 (≠ C203), P213 (= P204), T218 (= T209), I239 (≠ T230), A240 (= A231), R241 (= R232), H288 (= H285), G290 (≠ A287)
Sites not aligning to the query:
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
37% identity, 80% coverage: 62:320/322 of query aligns to 65:322/332 of 6biiA
- active site: L99 (≠ A96), R240 (= R232), D264 (= D256), E269 (= E261), H287 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ T72), T103 (≠ V100), G156 (= G152), F157 (≠ G153), G158 (= G154), R159 (≠ S155), I160 (≠ L156), A179 (vs. gap), R180 (vs. gap), S181 (vs. gap), K183 (vs. gap), V211 (≠ C203), P212 (= P204), E216 (= E208), T217 (= T209), V238 (≠ T230), A239 (= A231), R240 (= R232), D264 (= D256), H287 (= H285), G289 (≠ A287)
3kb6B Crystal structure of d-lactate dehydrogenase from aquifex aeolicus complexed with NAD and lactic acid (see paper)
30% identity, 87% coverage: 44:322/322 of query aligns to 47:331/334 of 3kb6B
- active site: S97 (≠ A96), R231 (= R232), D255 (= D256), E260 (= E261), H294 (= H285)
- binding lactic acid: F49 (≠ A46), S72 (≠ A71), V73 (≠ T72), G74 (= G73), Y96 (= Y95), R231 (= R232), H294 (= H285)
- binding nicotinamide-adenine-dinucleotide: V73 (≠ T72), Y96 (= Y95), V101 (= V100), G150 (= G154), R151 (≠ S155), I152 (≠ L156), D171 (vs. gap), V172 (vs. gap), P203 (= P204), T229 (= T230), A230 (= A231), R231 (= R232), H294 (= H285), A296 (= A287), Y297 (≠ W288)
5z20F The ternary structure of d-lactate dehydrogenase from pseudomonas aeruginosa with nadh and oxamate (see paper)
38% identity, 80% coverage: 65:321/322 of query aligns to 77:334/336 of 5z20F
- active site: S108 (≠ A96), R241 (= R232), D265 (= D256), E270 (= E261), H302 (= H285)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y107 (= Y95), G160 (= G154), Q161 (≠ S155), I162 (≠ L156), Y180 (≠ A174), D181 (≠ E175), P182 (≠ R176), C212 (= C203), P213 (= P204), T218 (= T209), T239 (= T230), G240 (≠ A231), R241 (= R232), H302 (= H285), A304 (= A287)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
36% identity, 78% coverage: 44:295/322 of query aligns to 44:288/304 of 1wwkA
- active site: S96 (≠ A96), R230 (= R232), D254 (= D256), E259 (= E261), H278 (= H285)
- binding nicotinamide-adenine-dinucleotide: V100 (= V100), G146 (= G152), F147 (≠ G153), G148 (= G154), R149 (≠ S155), I150 (≠ L156), Y168 (vs. gap), D169 (vs. gap), P170 (vs. gap), V201 (≠ C203), P202 (= P204), T207 (= T209), T228 (= T230), S229 (≠ A231), D254 (= D256), H278 (= H285), G280 (≠ A287)
2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
35% identity, 83% coverage: 53:320/322 of query aligns to 57:323/333 of 2dbqA
- active site: L100 (≠ A96), R241 (= R232), D265 (= D256), E270 (= E261), H288 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ T72), T104 (≠ V100), L158 (≠ G153), G159 (= G154), R160 (≠ S155), I161 (≠ L156), S180 (vs. gap), R181 (vs. gap), T182 (vs. gap), A211 (≠ H202), V212 (≠ C203), P213 (= P204), T218 (= T209), I239 (≠ T230), A240 (= A231), R241 (= R232), D265 (= D256), H288 (= H285), G290 (≠ A287)
O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
35% identity, 83% coverage: 53:320/322 of query aligns to 57:323/334 of O58320
8atiA Human ctbp2(31-364) in complex with rai2 peptide(315-322)
37% identity, 73% coverage: 58:293/322 of query aligns to 60:297/330 of 8atiA
- binding nicotinamide-adenine-dinucleotide: S74 (≠ T72), T102 (≠ V100), G155 (= G152), G157 (= G154), R158 (≠ S155), T159 (≠ L156), D178 (vs. gap), P179 (vs. gap), Y180 (vs. gap), H210 (= H202), C211 (= C203), N212 (≠ P204), A238 (≠ T230), R240 (= R232), H289 (= H285), A291 (= A287), W292 (= W288)
Sites not aligning to the query:
4lcjA Ctbp2 in complex with substrate mtob (see paper)
37% identity, 73% coverage: 58:293/322 of query aligns to 60:297/330 of 4lcjA
- active site: A98 (= A96), R240 (= R232), D264 (= D256), E269 (= E261), H289 (= H285)
- binding 4-(methylsulfanyl)-2-oxobutanoic acid: I72 (≠ A70), G73 (≠ A71), S74 (≠ T72), G75 (= G73), R240 (= R232), H289 (= H285), W292 (= W288)
- binding nicotinamide-adenine-dinucleotide: S74 (≠ T72), T102 (≠ V100), I154 (≠ V151), G155 (= G152), G157 (= G154), R158 (≠ S155), T159 (≠ L156), D178 (vs. gap), Y180 (vs. gap), H210 (= H202), C211 (= C203), N212 (≠ P204), N214 (≠ T206), N217 (≠ T209), A238 (≠ T230), A239 (= A231), R240 (= R232), H289 (= H285), W292 (= W288)
Sites not aligning to the query:
6p2iA Acyclic imino acid reductase (bsp5) in complex with NADPH and d-arg (see paper)
34% identity, 84% coverage: 39:308/322 of query aligns to 41:297/307 of 6p2iA
- binding d-arginine: E51 (≠ N49), T73 (≠ A71), T74 (= T72), S75 (≠ G73), Y97 (= Y95), W277 (= W288)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S98 (≠ A96), V102 (= V100), G149 (= G152), I150 (≠ G153), G151 (= G154), Q152 (≠ S155), I153 (≠ L156), N172 (≠ E175), K173 (≠ R176), S174 (≠ K177), R176 (≠ A179), H199 (= H202), I200 (≠ C203), P201 (= P204), T206 (= T209), T227 (= T230), C228 (≠ A231), W277 (= W288)
4cukA Structure of salmonella d-lactate dehydrogenase in complex with nadh
32% identity, 79% coverage: 64:317/322 of query aligns to 69:327/330 of 4cukA
- active site: S101 (≠ A96), R234 (= R232), D258 (= D256), E263 (= E261), H295 (= H285)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y100 (= Y95), G153 (= G154), K154 (≠ S155), I155 (≠ L156), F173 (≠ A174), D174 (≠ E175), P175 (vs. gap), H204 (= H202), C205 (= C203), P206 (= P204), N211 (≠ T209), T232 (= T230), Y260 (≠ L258), H295 (= H285), A297 (= A287)
P56545 C-terminal-binding protein 2; CtBP2 from Homo sapiens (Human)
37% identity, 73% coverage: 58:293/322 of query aligns to 92:329/445 of P56545
- IGFGRT 186:191 (≠ VGGGSL 151:156) binding
- D210 (vs. gap) binding
- 243:249 (vs. 203:209, 29% identical) binding
- AAR 270:272 (≠ TAR 230:232) binding
- D296 (= D256) binding
- HTAW 321:324 (≠ HVAW 285:288) binding
5v7nA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and 2-keto-d-gluconic acid (see paper)
38% identity, 85% coverage: 43:315/322 of query aligns to 44:308/319 of 5v7nA
- active site: L95 (≠ A96), R229 (= R232), D253 (= D256), E258 (= E261), H276 (= H285)
- binding 2-keto-D-gluconic acid: G70 (≠ A71), V71 (≠ T72), G72 (= G73), R229 (= R232), H276 (= H285), S279 (≠ W288), R285 (≠ M294)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V71 (≠ T72), V99 (= V100), L149 (≠ G153), G150 (= G154), R151 (≠ S155), I152 (≠ L156), T171 (≠ L172), R172 (≠ Q173), V200 (≠ C203), P201 (= P204), S205 (≠ E208), T206 (= T209), V227 (≠ T230), G228 (≠ A231), R229 (= R232), H276 (= H285), A278 (= A287)
5v6qB Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and malonate (see paper)
38% identity, 85% coverage: 43:315/322 of query aligns to 45:309/319 of 5v6qB
- active site: L96 (≠ A96), R230 (= R232), D254 (= D256), E259 (= E261), H277 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V72 (≠ T72), V100 (= V100), F148 (≠ V151), L150 (≠ G153), G151 (= G154), R152 (≠ S155), I153 (≠ L156), T172 (≠ L172), R173 (≠ Q173), V201 (≠ C203), P202 (= P204), S206 (≠ E208), T207 (= T209), V228 (≠ T230), G229 (≠ A231), R230 (= R232), H277 (= H285), A279 (= A287)
5j23A Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with 2'-phospho-adp-ribose (see paper)
38% identity, 85% coverage: 43:315/322 of query aligns to 43:307/318 of 5j23A
- active site: L94 (≠ A96), R228 (= R232), D252 (= D256), E257 (= E261), H275 (= H285)
- binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4r,5r)-3,4,5-trihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: V70 (≠ T72), L148 (≠ G153), G149 (= G154), R150 (≠ S155), I151 (≠ L156), T170 (≠ L172), R171 (≠ Q173), P200 (= P204), S204 (≠ E208), T205 (= T209), R228 (= R232)
5v7gA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
38% identity, 85% coverage: 43:315/322 of query aligns to 43:307/317 of 5v7gA
- active site: L94 (≠ A96), R228 (= R232), D252 (= D256), E257 (= E261), H275 (= H285)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V70 (≠ T72), V98 (= V100), F146 (≠ V151), L148 (≠ G153), G149 (= G154), R150 (≠ S155), I151 (≠ L156), T170 (≠ L172), R171 (≠ Q173), V199 (≠ C203), P200 (= P204), S204 (≠ E208), T205 (= T209), V226 (≠ T230), G227 (≠ A231), R228 (= R232), H275 (= H285), A277 (= A287)
- binding oxalate ion: G69 (≠ A71), V70 (≠ T72), G71 (= G73), R228 (= R232), H275 (= H285)
1hl3A Ctbp/bars in ternary complex with NAD(h) and pidlskk peptide (see paper)
34% identity, 73% coverage: 58:293/322 of query aligns to 61:298/331 of 1hl3A
- active site: S99 (≠ A96), R241 (= R232), D265 (= D256), E270 (= E261), H290 (= H285)
- binding nicotinamide-adenine-dinucleotide: T103 (≠ V100), G158 (= G154), R159 (≠ S155), V160 (≠ L156), D179 (≠ E175), Y181 (vs. gap), H211 (= H202), C212 (= C203), G213 (≠ P204), N218 (≠ T209), T239 (= T230), A240 (= A231), R241 (= R232), D265 (= D256), H290 (= H285)
Sites not aligning to the query:
1hkuA Ctbp/bars: a dual-function protein involved in transcription corepression and golgi membrane fission (see paper)
34% identity, 73% coverage: 58:293/322 of query aligns to 61:298/331 of 1hkuA
- active site: S99 (≠ A96), R241 (= R232), D265 (= D256), E270 (= E261), H290 (= H285)
- binding nicotinamide-adenine-dinucleotide: S75 (≠ T72), T103 (≠ V100), G156 (= G152), G158 (= G154), R159 (≠ S155), V160 (≠ L156), Y178 (≠ A174), D179 (≠ E175), P180 (vs. gap), Y181 (vs. gap), C212 (= C203), N218 (≠ T209), T239 (= T230), A240 (= A231), R241 (= R232), H290 (= H285), W293 (= W288)
Sites not aligning to the query:
4u6sA Ctbp1 in complex with substrate phenylpyruvate (see paper)
34% identity, 73% coverage: 58:293/322 of query aligns to 61:298/328 of 4u6sA
- active site: S99 (≠ A96), R241 (= R232), D265 (= D256), E270 (= E261), H290 (= H285)
- binding nicotinamide-adenine-dinucleotide: T103 (≠ V100), G156 (= G152), G158 (= G154), R159 (≠ S155), V160 (≠ L156), Y178 (≠ A174), D179 (≠ E175), P180 (≠ R176), Y181 (≠ K177), H211 (= H202), C212 (= C203), G213 (≠ P204), N218 (≠ T209), T239 (= T230), A240 (= A231), R241 (= R232), H290 (= H285), W293 (= W288)
- binding 3-phenylpyruvic acid: I73 (≠ A70), G74 (≠ A71), S75 (≠ T72), G76 (= G73), R241 (= R232), W293 (= W288)
Sites not aligning to the query:
4u6qA Ctbp1 bound to inhibitor 2-(hydroxyimino)-3-phenylpropanoic acid (see paper)
34% identity, 73% coverage: 58:293/322 of query aligns to 61:298/328 of 4u6qA
- active site: S99 (≠ A96), R241 (= R232), D265 (= D256), E270 (= E261), H290 (= H285)
- binding (2E)-2-(hydroxyimino)-3-phenylpropanoic acid: I73 (≠ A70), G74 (≠ A71), S75 (≠ T72), G76 (= G73), R241 (= R232), H290 (= H285), W293 (= W288)
- binding 1,4-dihydronicotinamide adenine dinucleotide: S75 (≠ T72), T103 (≠ V100), G156 (= G152), R159 (≠ S155), V160 (≠ L156), Y178 (≠ A174), D179 (≠ E175), P180 (≠ R176), Y181 (≠ K177), H211 (= H202), C212 (= C203), G213 (≠ P204), N218 (≠ T209), T239 (= T230), A240 (= A231), R241 (= R232), H290 (= H285), W293 (= W288)
Sites not aligning to the query:
Query Sequence
>Dsui_0124 Dsui_0124 lactate dehydrogenase-like oxidoreductase
MHRIVYLERESIRAEVRRPAFAHEWTEYARTTAAQLEERLAGASIAIVNKLPISGALMAR
LPQLKMVAVAATGTNNVDLEAARRQGIVVSNIQGYAVHTVPEHVFSLLLALSRNLLAYRQ
SVAEGRWQRAEQFCFFDHPIRDLHGATLGVVGGGSLGQGVVRLAQAFGMKVLQAERKGAA
VVRPGYTAFATVLAEADALSLHCPLTAETRHLIGAAELQAMKPSALLINTARGGLVDEAA
LARALREGWIAGAGFDVLTAEPPTDDHPLLSPDLLAAPNFLLTPHVAWASAPAMQALADQ
LIDNLEAFARGAMTPGAANRVA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory