SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Dsui_0124 Dsui_0124 lactate dehydrogenase-like oxidoreductase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
38% identity, 83% coverage: 53:320/322 of query aligns to 57:323/334 of 5aovA

query
sites
5aovA

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active site: L100 (≠ A96), R241 (= R232), D265 (= D256), E270 (= E261), H288 (= H285)
binding glyoxylic acid: Y74 (≠ A70), A75 (= A71), A75 (= A71), V76 (≠ T72), G77 (= G73), R241 (= R232), H288 (= H285)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ T72), G77 (= G73), L100 (≠ A96), T104 (≠ V100), G157 (= G152), F158 (≠ G153), G159 (= G154), R160 (≠ S155), I161 (≠ L156), Y179 (vs. gap), S180 (vs. gap), R181 (vs. gap), T182 (vs. gap), K184 (vs. gap), A211 (≠ H202), V212 (≠ C203), P213 (= P204), T218 (= T209), I239 (≠ T230), A240 (= A231), R241 (= R232), D265 (= D256), H288 (= H285), G290 (≠ A287)

Sites not aligning to the query:

binding glyoxylic acid: 52, 53, 53

6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
37% identity, 80% coverage: 62:320/322 of query aligns to 65:322/332 of 6biiA

query
sites
6biiA

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active site: L99 (≠ A96), R240 (= R232), D264 (= D256), E269 (= E261), H287 (= H285)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ T72), G76 (= G73), L99 (≠ A96), T103 (≠ V100), G156 (= G152), F157 (≠ G153), G158 (= G154), R159 (≠ S155), I160 (≠ L156), A179 (vs. gap), R180 (vs. gap), S181 (vs. gap), K183 (vs. gap), A210 (≠ H202), V211 (≠ C203), P212 (= P204), E216 (= E208), T217 (= T209), V238 (≠ T230), A239 (= A231), R240 (= R232), D264 (= D256), H287 (= H285), G289 (≠ A287)

3kb6B Crystal structure of d-lactate dehydrogenase from aquifex aeolicus complexed with NAD and lactic acid (see paper)
30% identity, 87% coverage: 44:322/322 of query aligns to 47:331/334 of 3kb6B

query
sites
3kb6B

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active site: S97 (≠ A96), R231 (= R232), D255 (= D256), E260 (= E261), H294 (= H285)
binding lactic acid: F49 (≠ A46), V50 (≠ I47), S72 (≠ A71), V73 (≠ T72), G74 (= G73), Y96 (= Y95), R231 (= R232), H294 (= H285), Y297 (≠ W288)
binding nicotinamide-adenine-dinucleotide: V73 (≠ T72), Y96 (= Y95), V101 (= V100), T149 (≠ G153), G150 (= G154), R151 (≠ S155), I152 (≠ L156), Y170 (vs. gap), D171 (vs. gap), V172 (vs. gap), H201 (= H202), V202 (≠ C203), P203 (= P204), T208 (= T209), T229 (= T230), A230 (= A231), R231 (= R232), D255 (= D256), H294 (= H285), A296 (= A287), Y297 (≠ W288)

5z20F The ternary structure of d-lactate dehydrogenase from pseudomonas aeruginosa with nadh and oxamate (see paper)
38% identity, 80% coverage: 65:321/322 of query aligns to 77:334/336 of 5z20F

query
sites
5z20F

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A

-

A

R

N

N

R

R

V

V

active site: S108 (≠ A96), R241 (= R232), D265 (= D256), E270 (= E261), H302 (= H285)
binding 1,4-dihydronicotinamide adenine dinucleotide: Y107 (= Y95), V112 (= V100), G158 (= G152), G160 (= G154), Q161 (≠ S155), I162 (≠ L156), Y180 (≠ A174), D181 (≠ E175), P182 (≠ R176), H211 (= H202), C212 (= C203), P213 (= P204), T218 (= T209), T239 (= T230), G240 (≠ A231), R241 (= R232), D265 (= D256), H302 (= H285), A304 (= A287), F305 (≠ W288)

1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
36% identity, 78% coverage: 44:295/322 of query aligns to 44:288/304 of 1wwkA

query
sites
1wwkA

S

A

I

I

A

I

I

V

V

R

N

S

K

K

L

P

P

K

I

V

S

T

G

R

A

R

L

V

M

I

A

E

R

S

L

A

P

P

Q

K

L

L

K

K

M

V

V

I

A

A

V

R

A

A

A

G

T

V

G

G

T

L

N

D

N

N

V

I

D

D

L

V

E

E

A

A

A

A

R

K

R

E

Q

K

G

G

I

I

V

E

V

V

S

V

N

N

I

A

Q

P

G

A

Y

A

A
x
S

V

S

H

R

T

S

V
|
V

P

A

E

E

H

L

V

A

F

V

S

G

L

L

L

M

L

F

A

S

L

V

S

A

R

R

N

K

L

I

L

A

A

F

Y

A

R

D

Q

R

S

K

V

M

A

R

E

E

G

G

R

V

W

W

Q

A

R

K

A

K

E

E

Q

A

F

M

C

G

F

I

F

-

D

-

H

-

P

-

I

-

R

-

D

E

L

L

H

E

G

G

A

K

T

T

L

I

G

G

V

I

V

I

G
|
G

G
x
F

G
|
G

S
x
R

L
x
I

G

G

Q

Y

G

Q

V

V

V

A

R

K

L

I

A

A

Q

N

A

A

F

L

G

G

M

M

K

N

V

I

L

L

-

L

-
x
Y

-
x
D

-
x
P

-
x
Y

-

P

Q

N

A

E

E

E

R

R

K

-

G

-

A

A

A

K

V

E

V

V

R

N

P

G

G

K

Y

F

T

V

A

D

F

L

A

E

T

T

V

L

L

L

A

K

E

E

A

S

D

D

A

V

L

V

S

T

L

I

H
|
H

C
x
V

P
|
P

L

L

T

V

A

E

E

S

T
|
T

R

Y

H

H

L

L

I

I

G

N

A

E

A

E

E

R

L

L

Q

K

A

L

M

M

K

K

P

K

S

T

A

A

L

I

L

L

I

I

N

N

T
|
T

A
x
S

R
|
R

G

G

G

P

L

V

V

V

D

D

E

T

A

N

A

A

L

L

A

V

R

K

A

A

L

L

R

K

E

E

G

G

W

W

I

I

A

A

G

G

A

A

G

G

F

L

D
|
D

V

V

L

F

T

E

A

E

E
|
E

P

P

P

L

T

P

D

K

D

D

H

H

P

P

L

L

L

T

S

K

P

F

D

D

L

-

L

-

A

-

A

-

P

-

N

N

F

V

L

V

L

L

T

T

P

P

H
|
H

V

I

A
x
G

W
x
A

A

S

S

T

A

V

P

E

A

A

M

Q

Q

E

active site: S96 (≠ A96), R230 (= R232), D254 (= D256), E259 (= E261), H278 (= H285)
binding nicotinamide-adenine-dinucleotide: V100 (= V100), G146 (= G152), F147 (≠ G153), G148 (= G154), R149 (≠ S155), I150 (≠ L156), Y168 (vs. gap), D169 (vs. gap), P170 (vs. gap), Y171 (vs. gap), H200 (= H202), V201 (≠ C203), P202 (= P204), T207 (= T209), T228 (= T230), S229 (≠ A231), R230 (= R232), D254 (= D256), H278 (= H285), G280 (≠ A287), A281 (≠ W288)

2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
35% identity, 83% coverage: 53:320/322 of query aligns to 57:323/333 of 2dbqA

query
sites
2dbqA

I

I

S

D

G

K

A

E

L

V

M

F

A

E

R

N

L

A

P

P

Q

K

L

L

K

R

M

I

V

V

A

A

V

N

A

Y

A

A

T
x
V

G
|
G

T

Y

N

D

N

N

V

I

D

D

L

I

E

E

A

E

A

A

R

T

R

K

Q

R

G

G

I

I

V

Y

V

V

S

T

N

N

I

T

Q

P

G

D

Y

V

A
x
L

V

T

H

D

T

A

V
x
T

P

A

E

D

H

L

V

A

F

F

S

A

L

L

L

L

L

L

A

A

L

T

S

A

R

R

N

H

L

V

L

V

A

K

Y

G

R

D

Q

R

S

F

V

V

A

R

E

S

G

G

R

E

W

W

Q

K

R

K

-

R

-

G

-

V

-

A

-

W

-

H

A

P

E

K

Q

W

F

F

C

L

F

G

F

Y

D

D

H

-

P

-

I

-

R

-

D

-

L

V

H

Y

G

G

A

K

T

T

L

I

G

G

V

I

V

I

G
|
G

G
x
L

G
|
G

S
x
R

L
x
I

G

G

Q

Q

G

A

V

I

V

A

R

K

L

R

A

A

Q

K

A

G

F

F

G

N

M

M

K

R

V

I

L

L

-

Y

-
x
Y

-
x
S

-
x
R

-
x
T

-

R

-
x
K

-

E

Q

E

A

V

E

E

R

R

K

E

G

L

A

N

A

A

V

E

V

F

R

K

P

P

G

-

Y

-

T

-

A

-

F

L

A

E

T

D

V

L

L

L

A

R

E

E

A

S

D

D

A

F

L

V

S

V

L

L

H
x
A

C
x
V

P
|
P

L

L

T

T

A

R

E

E

T
|
T

R

Y

H

H

L

L

I

I

G

N

A

E

A

E

E

R

L

L

Q

K

A

L

M

M

K

K

P

K

S

T

A

A

L

I

L

L

I

I

N

N

T
x
I

A
|
A

R
|
R

G

G

G

K

L

V

V

V

D

D

E

T

A

N

A

A

L

L

A

V

R

K

A

A

L

L

R

K

E

E

G

G

W

W

I

I

A

A

G

G

A

A

G

G

F

L

D
|
D

V

V

L

F

T

E

A

E

E
|
E

P

-

P

-

T

-

D

-

D

-

H

-

P

P

L

Y

L

Y

S

N

P

E

D

E

L

L

L

F

A

K

A

L

P

D

N

N

F

V

L

V

L

L

T

T

P

P

H
|
H

V

I

A
x
G

W

S

A

A

S

S

A

F

P

G

A

A

M

R

Q

E

A

G

L

M

A

A

D

E

Q

L

L

V

I

A

D

K

N

N

L

L

E

I

A

A

F

F

A

K

R

R

G

G

A

E

M

I

T

P

P

P

G

T

A

L

A

V

N

N

R

R

active site: L100 (≠ A96), R241 (= R232), D265 (= D256), E270 (= E261), H288 (= H285)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ T72), G77 (= G73), L100 (≠ A96), T104 (≠ V100), G157 (= G152), L158 (≠ G153), G159 (= G154), R160 (≠ S155), I161 (≠ L156), Y179 (vs. gap), S180 (vs. gap), R181 (vs. gap), T182 (vs. gap), K184 (vs. gap), A211 (≠ H202), V212 (≠ C203), P213 (= P204), T218 (= T209), I239 (≠ T230), A240 (= A231), R241 (= R232), D265 (= D256), H288 (= H285), G290 (≠ A287)

O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
35% identity, 83% coverage: 53:320/322 of query aligns to 57:323/334 of O58320

query
sites
O58320

I

I

S

D

G

K

A

E

L

V

M

F

A

E

R

N

L

A

P

P

Q

K

L

L

K

R

M

I

V

V

A

A

V

N

A

Y

A

A

T

V

G

G

T

Y

N

D

N

N

V

I

D

D

L

I

E

E

A

E

A

A

R

T

R

K

Q

R

G

G

I

I

V

Y

V

V

S

T

N

N

I

T

Q

P

G

D

Y

V

A

L

V

T

H

D

T

A

V

T

P

A

E

D

H

L

V

A

F

F

S

A

L

L

L

L

L

L

A

A

L

T

S

A

R

R

N

H

L

V

L

V

A

K

Y

G

R

D

Q

R

S

F

V

V

A

R

E

S

G

G

R

E

W

W

Q

K

R

K

-

R

-

G

-

V

-

A

-

W

-

H

A

P

E

K

Q

W

F

F

C

L

F

G

F

Y

D

D

H

-

P

-

I

-

R

-

D

-

L

V

H

Y

G

G

A

K

T

T

L

I

G

G

V

I

V

I

G

G

G
x
L

G
|
G

S
x
R

L
x
I

G

G

Q

Q

G

A

V

I

V

A

R

K

L

R

A

A

Q

K

A

G

F

F

G

N

M

M

K

R

V

I

L

L

-

Y

-

Y

-
x
S

-
x
R

-
x
T

-

R

-

K

-

E

Q

E

A

V

E

E

R

R

K

E

G

L

A

N

A

A

V

E

V

F

R

K

P

P

G

-

Y

-

T

-

A

-

F

L

A

E

T

D

V

L

L

L

A

R

E

E

A

S

D

D

A

F

L

V

S

V

L

L

H

A

C

V

P

P

L

L

T

T

A

R

E

E

T

T

R

Y

H

H

L

L

I

I

G

N

A

E

A

E

E

R

L

L

Q

K

A

L

M

M

K

K

P

K

S

T

A

A

L

I

L

L

I

I

N

N

T
x
I

A
|
A

R
|
R

G

G

G

K

L

V

V

V

D

D

E

T

A

N

A

A

L

L

A

V

R

K

A

A

L

L

R

K

E

E

G

G

W

W

I

I

A

A

G

G

A

A

G

G

F

L

D

D

V

V

L

F

T

E

A

E

E

E

P

-

P

-

T

-

D

-

D

-

H

-

P

P

L

Y

L

Y

S

N

P

E

D

E

L

L

L

F

A

K

A

L

P

D

N

N

F

V

L

V

L

L

T

T

P

P

H
|
H

V
x
I

A
x
G

W

S

A

A

S

S

A

F

P

G

A

A

M

R

Q

E

A

G

L

M

A

A

D

E

Q

L

L

V

I

A

D

K

N

N

L

L

E

I

A

A

F

F

A

K

R

R

G

G

A

E

M

I

T

P

P

P

G

T

A

L

A

V

N

N

R

R

LGRI 158:161 (≠ GGSL 153:156) binding
SRT 180:182 (vs. gap) binding
IAR 239:241 (≠ TAR 230:232) binding
HIG 288:290 (≠ HVA 285:287) binding

4lcjA Ctbp2 in complex with substrate mtob (see paper)
37% identity, 73% coverage: 58:293/322 of query aligns to 60:297/330 of 4lcjA

query
sites
4lcjA

M

L

A

E

R

K

L

F

P

K

Q

A

L

L

K

R

M

V

V

I

A

V

V
x
R

A
x
I

A
x
G

T
x
S

G
|
G

T

Y

N

D

N

N

V

V

D

D

L

I

E

K

A

A

A

A

R

G

R

E

Q

L

G

G

I

I

V

A

V

V

S

C

N

N

I

I

Q

P

G

S

Y

A

A
|
A

V

V

H

E

T

E

V
x
T

P

A

E

D

H

S

V

T

F

I

S

C

L

H

L

I

L

L

A

N

L

L

S

Y

R

R

N

R

L

N

L

T

A

W

Y

L

R

Y

Q

Q

S

A

V

L

A

R

E

E

G

G

-

T

R

R

W

V

Q

Q

R

S

A

V

E

E

Q

Q

F

I

C

R

F

E

F

V

D

A

H

S

P

G

I

A

R

A

D

R

L

I

H

R

G

G

A

E

T

T

L

L

G

G

V

L

V
x
I

G
|
G

G
x
F

G
|
G

S
x
R

L
x
T

G

G

Q

Q

G

A

V

V

V

A

R

V

L

R

A

A

Q

K

A

A

F

F

G

G

M

F

K

S

V

V

-

I

-

F

-
x
Y

-
x
D

-
x
P

-
x
Y

L

L

Q

Q

A

D

E

G

R

I

K

E

G

R

A

S

A

L

V

G

V

V

R

Q

P

R

G

V

Y

Y

T

T

A

-

F

L

A

Q

T

D

V

L

L

L

A

Y

E

Q

A

S

D

D

A

C

L

V

S

S

L

L

H
|
H

C
|
C

P
x
N

L

L

T
x
N

A

E

E

H

T
x
N

R

H

H

H

L

L

I

I

G

N

A

D

A

F

E

T

L

I

Q

K

A

Q

M

M

K

R

P

Q

S

G

A

A

L

F

L

L

I

V

N

N

T
x
A

A
|
A

R
|
R

G

G

G

G

L

L

V

V

D

D

E

E

A

K

A

A

L

L

A

A

R

Q

A

A

L

L

R

K

E

E

G

G

W

R

I

I

A

R

G

G

A

A

G

A

F

L

D
|
D

V

V

L

H

T

E

A

S

E
|
E

P

P

P

-

T

-

D

-

D

-

H

F

P

S

L

F

L

A

S

Q

P

G

D

P

L

L

L

K

A

D

A

A

P

P

N

N

F

L

L

I

L

C

T

T

P

P

H
|
H

V

T

A
|
A

W
|
W

A

Y

S

S

A

E

P

Q

A

A

active site: A98 (= A96), R240 (= R232), D264 (= D256), E269 (= E261), H289 (= H285)
binding 4-(methylsulfanyl)-2-oxobutanoic acid: R71 (≠ V69), I72 (≠ A70), G73 (≠ A71), S74 (≠ T72), G75 (= G73), R240 (= R232), H289 (= H285), W292 (= W288)
binding nicotinamide-adenine-dinucleotide: S74 (≠ T72), G75 (= G73), T102 (≠ V100), I154 (≠ V151), G155 (= G152), F156 (≠ G153), G157 (= G154), R158 (≠ S155), T159 (≠ L156), Y177 (vs. gap), D178 (vs. gap), P179 (vs. gap), Y180 (vs. gap), H210 (= H202), C211 (= C203), N212 (≠ P204), N214 (≠ T206), N217 (≠ T209), A238 (≠ T230), A239 (= A231), R240 (= R232), D264 (= D256), H289 (= H285), A291 (= A287), W292 (= W288)

Sites not aligning to the query:

binding 4-(methylsulfanyl)-2-oxobutanoic acid: 50, 51

6p2iA Acyclic imino acid reductase (bsp5) in complex with NADPH and d-arg (see paper)
34% identity, 84% coverage: 39:308/322 of query aligns to 41:297/307 of 6p2iA

query
sites
6p2iA

R

R

L

L

A

S

G

S

A

T

S

D

I

I

A

A

I

I

V

V

N
x
E

K

W

L

T

P

S

I

I

S

T

G

K

A

E

L

M

M

I

A

E

R

K

L

I

P

S

Q

R

L

L

K

K

M

Y

V

L

A

I

V

T

A
x
I

A
x
T

T
|
T

G
x
S

T

Y

N

D

N

Y

V

I

D

D

L

V

E

N

A

S

A

L

R

K

R

D

Q

N

G

E

I

I

V

M

V

V

S

S

N

N

I

C

Q

P

G

Q

Y
|
Y

A
x
S

V

K

H

Q

T

A

V
|
V

P

A

E

E

H

H

V

V

F

F

S

A

L

L

L

L

L

F

A

A

L

V

S

N

R

R

N

K

L

I

L

L

A

Q

Y

A

R

D

Q

E

S

T

V

C

A

R

E

K

G

G

R

L

W

S

Q

H

R

I

A

Y

E

P

Q

P

F

F

-

L

C

C

F

-

F

-

D

-

H

S

P

E

I

I

R

R

D

D

L

-

H

-

G

-

A

K

T

T

L

I

G

G

V

L

V

I

G
|
G

G
x
I

G
|
G

S
x
Q

L
x
I

G

G

Q

Q

G

T

V

V

V

A

R

E

L

I

A

A

Q

N

A

A

F

F

G

Q

M

M

K

K

V

V

L

I

Q

G

A
x
L

E
x
N

R
x
K

K
x
S

G

K

A
x
R

A

N

V

V

V

K

R

G

P

I

G

Q

Y

Q

T

V

A

D

F

I

A

T

T

E

V

L

L

M

A

K

E

K

A

S

D

D

A

I

L

I

S

S

L

L

H
|
H

C
x
I

P
|
P

L

R

T

N

A

A

E

D

T
|
T

R

E

H

I

L

I

I

L

G

T

A

E

A

K

E

L

L

L

Q

S

A

L

M

M

K

K

P

P

S

D

A

A

L

V

L

L

I

I

N

N

T
|
T

A
x
C

R
|
R

G

G

G

N

L

L

V

I

D

D

E

E

A

Q

A

A

L

L

A

Y

R

S

A

V

L

L

R

K

E

Q

G

N

W

R

I

I

A

R

G

G

A

A

G

G

F

L

D
|
D

V

D

L

L

T

T

A

Y

E

-

P

-

P

-

T

Y

D

K

D

D

H

N

P

P

L

I

L

I

S

G

P

L

D

N

L

-

L

-

A

-

A

-

P

-

N

N

F

V

L

V

L

L

T

T

P

P

H

G

V

S

A
|
A

W
|
W

A

Y

S

S

A

Y

P

E

A

A

M

R

Q

E

A

K

L
x
N

A

M

D

Y

Q

E

L

L

I

I

D

E

N

N

L

I

E

E

A

S

F

Y

binding d-arginine: E51 (≠ N49), I72 (≠ A70), T73 (≠ A71), T74 (= T72), S75 (≠ G73), Y97 (= Y95), R229 (= R232), W277 (= W288), N286 (≠ L297)
binding nadp nicotinamide-adenine-dinucleotide phosphate: T74 (= T72), S75 (≠ G73), S98 (≠ A96), V102 (= V100), G149 (= G152), I150 (≠ G153), G151 (= G154), Q152 (≠ S155), I153 (≠ L156), L171 (≠ A174), N172 (≠ E175), K173 (≠ R176), S174 (≠ K177), R176 (≠ A179), H199 (= H202), I200 (≠ C203), P201 (= P204), T206 (= T209), T227 (= T230), C228 (≠ A231), D253 (= D256), A276 (= A287), W277 (= W288)

Sites not aligning to the query:

binding d-arginine: 10

4cukA Structure of salmonella d-lactate dehydrogenase in complex with nadh
32% identity, 79% coverage: 64:317/322 of query aligns to 69:327/330 of 4cukA

query
sites
4cukA

L

V

K

K

M

Y

V

I

A

A

V

L

A

R

A

C

T

A

G

G

T

F

N

N

N

N

V

V

D

D

L

L

E

D

A

A

A

A

R

K

R

E

Q

L

G

G

I

L

V

Q

V

V

S

V

N

R

I

V

Q

P

G

A

Y
|
Y

A
x
S

V

P

H

E

T

A

V
|
V

P

A

E

E

H

H

V

A

F

I

S

G

L

M

L

M

L

M

A

T

L

L

S

N

R

R

N

R

L

I

-

H

L

R

A

A

Y

Y

R

Q

Q

R

S

T

V

-

A

-

E

-

G

-

R

-

W

-

Q

-

R

R

A

D

E

A

Q

N

F

F

C

S

F

L

F

E

D

G

H

L

P

T

I

G

R

F

D

T

L

M

H

H

G

G

A

K

T

T

L

A

G

G

V

V

V
x
I

G
|
G

G
x
T

G
|
G

S
x
K

L
x
I

G

G

Q

V

G

A

V

A

V

L

R

R

L

I

A

L

Q

K

A

G

F

F

G

G

M

M

K

R

V

L

L

L

Q

A

A
x
F

E
x
D

-
x
P

R
x
Y

K

P

G

S

A

T

A

A

V

A

V

L

R

D

P

L

G

G

-

V

-

E

Y

Y

T

V

A

D

F

L

A

Q

T

T

V

L

L

F

A

A

E

E

A

S

D

D

A

V

L

I

S

S

L

L

H
|
H

C
|
C

P
|
P

L

L

T

T

A

P

E

E

T
x
N

R

Y

H

H

L

L

I

L

G

N

A

H

A

A

E

A

L

F

Q

D

A

Q

M

M

K

K

P

N

S

G

A

V

L

M

L

I

I

I

N

N

T
|
T

A
x
S

R
|
R

G

G

G

A

L

L

V

I

D

D

E

S

A

Q

A

A

L

A

A

I

R

E

A

A

L

L

R

K

E

N

G

Q

W

K

I

I

A

G

G

S

A

L

G

G

F

M

D
|
D

V

V

L
x
Y

T

E

A

N

E
|
E

P

R

P

D

T

L

-

F

-

F

D

E

D

D

H

K

P

S

-

V

-

D

L

V

L

I

S

Q

P

D

D

D

L

V

L

F

-

R

-

R

-

L

-

S

A

A

A

C

P

H

N

N

F

V

L

L

L

F

T

T

P

G

H
|
H

V

Q

A
|
A

W
x
F

A

L

S

T

A

A

P

E

A

A

M

L

Q

I

A

S

L

I

A

S

D

E

Q

T

L

T

I

L

D

Q

N

N

L

L

E

S

A

Q

F

L

A

E

R

K

G

G

A

E

M

A

T

C

P

P

G

N

A

A

active site: S101 (≠ A96), R234 (= R232), D258 (= D256), E263 (= E261), H295 (= H285)
binding 1,4-dihydronicotinamide adenine dinucleotide: Y100 (= Y95), V105 (= V100), I150 (≠ V151), G151 (= G152), T152 (≠ G153), G153 (= G154), K154 (≠ S155), I155 (≠ L156), F173 (≠ A174), D174 (≠ E175), P175 (vs. gap), Y176 (≠ R176), H204 (= H202), C205 (= C203), P206 (= P204), N211 (≠ T209), T232 (= T230), S233 (≠ A231), D258 (= D256), Y260 (≠ L258), H295 (= H285), A297 (= A287), F298 (≠ W288)

P56545 C-terminal-binding protein 2; CtBP2 from Homo sapiens (Human)
37% identity, 73% coverage: 58:293/322 of query aligns to 92:329/445 of P56545

query
sites
P56545

M

L

A

E

R

K

L

F

P

K

Q

A

L

L

K

R

M

V

V

I

A

V

V

R

A

I

A

G

T

S

G

G

T

Y

N

D

N

N

V

V

D

D

L

I

E

K

A

A

A

A

R

G

R

E

Q

L

G

G

I

I

V

A

V

V

S

C

N

N

I

I

Q

P

G

S

Y

A

A

A

V

V

H

E

T

E

V

T

P

A

E

D

H

S

V

T

F

I

S

C

L

H

L

I

L

L

A

N

L

L

S

Y

R

R

N

R

L

N

L

T

A

W

Y

L

R

Y

Q

Q

S

A

V

L

A

R

E

E

G

G

-

T

R

R

W

V

Q

Q

R

S

A

V

E

E

Q

Q

F

I

C

R

F

E

F

V

D

A

H

S

P

G

I

A

R

A

D

R

L

I

H

R

G

G

A

E

T

T

L

L

G

G

V

L

V
x
I

G
|
G

G
x
F

G
|
G

S
x
R

L
x
T

G

G

Q

Q

G

A

V

V

V

A

R

V

L

R

A

A

Q

K

A

A

F

F

G

G

M

F

K

S

V

V

-

I

-

F

-

Y

-
x
D

-

P

-

Y

L

L

Q

Q

A

D

E

G

R

I

K

E

G

R

A

S

A

L

V

G

V

V

R

Q

P

R

G

V

Y

Y

T

T

A

-

F

L

A

Q

T

D

V

L

L

L

A

Y

E

Q

A

S

D

D

A

C

L

V

S

S

L

L

H

H

C
|
C

P
x
N

L
|
L

T
x
N

A
x
E

E
x
H

T
x
N

R

H

H

H

L

L

I

I

G

N

A

D

A

F

E

T

L

I

Q

K

A

Q

M

M

K

R

P

Q

S

G

A

A

L

F

L

L

I

V

N

N

T
x
A

A
|
A

R
|
R

G

G

G

G

L

L

V

V

D

D

E

E

A

K

A

A

L

L

A

A

R

Q

A

A

L

L

R

K

E

E

G

G

W

R

I

I

A

R

G

G

A

A

G

A

F

L

D
|
D

V

V

L

H

T

E

A

S

E

E

P

P

P

F

T

S

D

-

D

-

H

-

P

-

L

F

L

A

S

Q

P

G

D

P

L

L

L

K

A

D

A

A

P

P

N

N

F

L

L

I

L

C

T

T

P

P

H
|
H

V
x
T

A
|
A

W
|
W

A

Y

S

S

A

E

P

Q

A

A

IGFGRT 186:191 (≠ VGGGSL 151:156) binding
D210 (vs. gap) binding
243:249 (vs. 203:209, 29% identical) binding
AAR 270:272 (≠ TAR 230:232) binding
D296 (= D256) binding
HTAW 321:324 (≠ HVAW 285:288) binding

5v7nA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and 2-keto-d-gluconic acid (see paper)
38% identity, 85% coverage: 43:315/322 of query aligns to 44:308/319 of 5v7nA

query
sites
5v7nA

A

S

S

G

I

I

A

A

I

V

V
x
S

N
x
G

K

K

L

L

P

P

I

V

S

P

G

-

A

-

L

L

M

M

A

D

R

A

L

F

P

P

Q

S

L

L

K

E

M

I

V

V

A

A

V

N

A
x
F

A
x
G

T
x
V

G
|
G

T

Y

N

D

N

G

V

V

D

D

L

V

E

S

A

R

A

A

R

A

R

A

Q

R

G

G

I

I

V

V

V

V

S

T

N

N

I

T

Q

P

G

D

Y

V

A
x
L

V

T

H

E

T

E

V
|
V

P

A

E

D

H

T

V

A

F

I

S

G

L

L

L

L

L

L

A

N

L

T

S

L

R

R

N

L

L

L

L

P

A

Q

Y

A

R

E

Q

Q

S

W

V

L

A

R

E

Q

G

G

R

R

W

W

Q

V

R

R

A

E

E

G

Q

A

F

F

C

P

F

L

F

-

D

-

H

S

P

P

I

L

R

-

D

S

L

L

H

R

G

G

A

R

T

T

L

V

G

G

V

L

V
x
F

G
|
G

G
x
L

G
|
G

S
x
R

L
x
I

G

G

Q

L

G

A

V

I

V

A

R

R

L

R

A

L

Q

E

A

A

F

F

G

G

M

V

K

S

V

I

-

A

-

Y

-
x
H

L
x
T

Q
x
R

A
x
T

E

P

R

R

K

E

G

G

A

L

A

-

V

-

V

-

R

-

P

-

G

G

Y

F

T

T

A

Y

F

H

A

P

T

T

V

L

-

V

-

G

L

M

A

A

E

E

A

A

-

V

D

D

A

T

L

L

S

I

L

V

H
x
I

C
x
V

P
|
P

L

G

T

T

A

A

E
x
S

T
|
T

R

L

H

K

L

A

I

V

G

N

A

A

A

D

E

V

L

L

Q

S

A

A

M

L

K

G

P

P

S

K

A

G

L

V

L

L

I

I

N

N

T
x
V

A
x
G

R
|
R

G

G

G

S

L

T

V

V

D

D

E

E

A

A

A

A

L

L

A

V

R

T

A

A

L

L

R

Q

E

N

G

G

W

T

I

I

A

A

G

G

A

A

G

G

F

L

D
|
D

V

V

L

F

T

E

A

N

E
|
E

P

P

P

N

T

V

D

P

D

E

H

A

P

-

L

-

L

-

S

-

P

-

D

-

L

L

L

L

A

S

A

F

P

P

N

N

F

V

L

S

L

L

T

L

P

P

H
|
H

V

V

A
|
A

W
x
S

A

A

S

S

A

V

P

V

A

T

M
x
R

Q

N

A

A

L
x
M

A

S

D

D

Q

L

L

V

I

V

D

D

N

N

L

L

E

K

A

A

-

W

F

F

A

S

R

T

G

G

-

E

A

A

M

L

T

T

P

P

active site: L95 (≠ A96), R229 (= R232), D253 (= D256), E258 (= E261), H276 (= H285)
binding 2-keto-D-gluconic acid: S49 (≠ V48), G50 (≠ N49), F69 (≠ A70), G70 (≠ A71), V71 (≠ T72), G72 (= G73), L95 (≠ A96), R229 (= R232), H276 (= H285), S279 (≠ W288), R285 (≠ M294), M288 (≠ L297)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V71 (≠ T72), G72 (= G73), V99 (= V100), F147 (≠ V151), G148 (= G152), L149 (≠ G153), G150 (= G154), R151 (≠ S155), I152 (≠ L156), H170 (vs. gap), T171 (≠ L172), R172 (≠ Q173), T173 (≠ A174), I199 (≠ H202), V200 (≠ C203), P201 (= P204), S205 (≠ E208), T206 (= T209), V227 (≠ T230), G228 (≠ A231), R229 (= R232), D253 (= D256), H276 (= H285), A278 (= A287)

5v6qB Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and malonate (see paper)
38% identity, 85% coverage: 43:315/322 of query aligns to 45:309/319 of 5v6qB

query
sites
5v6qB

A

S

S

G

I

I

A

A

I

V

V

S

N

G

K

K

L

L

P

P

I

V

S

P

G

-

A

-

L

L

M

M

A

D

R

A

L

F

P

P

Q

S

L

L

K

E

M

I

V

V

A

A

V

N

A

F

A

G

T
x
V

G
|
G

T

Y

N

D

N

G

V

V

D

D

L

V

E

S

A

R

A

A

R

A

R

A

Q

R

G

G

I

I

V

V

V

V

S

T

N

N

I

T

Q

P

G

D

Y

V

A
x
L

V

T

H

E

T

E

V
|
V

P

A

E

D

H

T

V

A

F

I

S

G

L

L

L

L

L

L

A

N

L

T

S

L

R

R

N

L

L

L

L

P

A

Q

Y

A

R

E

Q

Q

S

W

V

L

A

R

E

Q

G

G

R

R

W

W

Q

V

R

R

A

E

E

G

Q

A

F

F

C

P

F

L

F

-

D

-

H

S

P

P

I

L

R

-

D

S

L

L

H

R

G

G

A

R

T

T

L

V

G

G

V

L

V
x
F

G
|
G

G
x
L

G
|
G

S
x
R

L
x
I

G

G

Q

L

G

A

V

I

V

A

R

R

L

R

A

L

Q

E

A

A

F

F

G

G

M

V

K

S

V

I

-

A

-

Y

-
x
H

L
x
T

Q
x
R

A
x
T

E

P

R

R

K

E

G

G

A

L

A

-

V

-

V

-

R

-

P

-

G

G

Y

F

T

T

A

Y

F

H

A

P

T

T

V

L

-

V

-

G

L

M

A

A

E

E

A

A

-

V

D

D

A

T

L

L

S

I

L

V

H
x
I

C
x
V

P
|
P

L

G

T

T

A

A

E
x
S

T
|
T

R

L

H

K

L

A

I

V

G

N

A

A

A

D

E

V

L

L

Q

S

A

A

M

L

K

G

P

P

S

K

A

G

L

V

L

L

I

I

N

N

T
x
V

A
x
G

R
|
R

G

G

G

S

L

T

V

V

D

D

E

E

A

A

A

A

L

L

A

V

R

T

A

A

L

L

R

Q

E

N

G

G

W

T

I

I

A

A

G

G

A

A

G

G

F

L

D
|
D

V

V

L

F

T

E

A

N

E
|
E

P

P

P

N

T

V

D

P

D

E

H

A

P

-

L

-

L

-

S

-

P

-

D

-

L

L

L

L

A

S

A

F

P

P

N

N

F

V

L

S

L

L

T

L

P

P

H
|
H

V

V

A
|
A

W

S

A

A

S

S

A

V

P

V

A

T

M

R

Q

N

A

A

L

M

A

S

D

D

Q

L

L

V

I

V

D

D

N

N

L

L

E

K

A

A

-

W

F

F

A

S

R

T

G

G

-

E

A

A

M

L

T

T

P

P

active site: L96 (≠ A96), R230 (= R232), D254 (= D256), E259 (= E261), H277 (= H285)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V72 (≠ T72), G73 (= G73), V100 (= V100), F148 (≠ V151), G149 (= G152), L150 (≠ G153), G151 (= G154), R152 (≠ S155), I153 (≠ L156), H171 (vs. gap), T172 (≠ L172), R173 (≠ Q173), T174 (≠ A174), I200 (≠ H202), V201 (≠ C203), P202 (= P204), S206 (≠ E208), T207 (= T209), V228 (≠ T230), G229 (≠ A231), R230 (= R232), D254 (= D256), H277 (= H285), A279 (= A287)

5j23A Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with 2'-phospho-adp-ribose (see paper)
38% identity, 85% coverage: 43:315/322 of query aligns to 43:307/318 of 5j23A

query
sites
5j23A

A

S

S

G

I

I

A

A

I

V

V

S

N

G

K

K

L

L

P

P

I

V

S

P

G

-

A

-

L

L

M

M

A

D

R

A

L

F

P

P

Q

S

L

L

K

E

M

I

V

V

A

A

V

N

A

F

A

G

T
x
V

G
|
G

T

Y

N

D

N

G

V

V

D

D

L

V

E

S

A

R

A

A

R

A

R

A

Q

R

G

G

I

I

V

V

V

V

S

T

N

N

I

T

Q

P

G

D

Y

V

A
x
L

V

T

H

E

T

E

V

V

P

A

E

D

H

T

V

A

F

I

S

G

L

L

L

L

L

L

A

N

L

T

S

L

R

R

N

L

L

L

L

P

A

Q

Y

A

R

E

Q

Q

S

W

V

L

A

R

E

Q

G

G

R

R

W

W

Q

V

R

R

A

E

E

G

Q

A

F

F

C

P

F

L

F

-

D

-

H

S

P

P

I

L

R

-

D

S

L

L

H

R

G

G

A

R

T

T

L

V

G

G

V

L

V
x
F

G
|
G

G
x
L

G
|
G

S
x
R

L
x
I

G

G

Q

L

G

A

V

I

V

A

R

R

L

R

A

L

Q

E

A

A

F

F

G

G

M

V

K

S

V

I

-

A

-

Y

-
x
H

L
x
T

Q
x
R

A
x
T

E

P

R

R

K

E

G

G

A

L

A

-

V

-

V

-

R

-

P

-

G

G

Y

F

T

T

A

Y

F

H

A

P

T

T

V

L

-

V

-

G

L

M

A

A

E

E

A

A

-

V

D

D

A

T

L

L

S

I

L

V

H
x
I

C
x
V

P
|
P

L

G

T

T

A

A

E
x
S

T
|
T

R

L

H

K

L

A

I

V

G

N

A

A

A

D

E

V

L

L

Q

S

A

A

M

L

K

G

P

P

S

K

A

G

L

V

L

L

I

I

N

N

T
x
V

A
x
G

R
|
R

G

G

G

S

L

T

V

V

D

D

E

E

A

A

A

A

L

L

A

V

R

T

A

A

L

L

R

Q

E

N

G

G

W

T

I

I

A

A

G

G

A

A

G

G

F

L

D
|
D

V

V

L

F

T

E

A

N

E
|
E

P

P

P

N

T

V

D

P

D

E

H

A

P

-

L

-

L

-

S

-

P

-

D

-

L

L

L

L

A

S

A

F

P

P

N

N

F

V

L

S

L

L

T

L

P

P

H
|
H

V

V

A

A

W

S

A

A

S

S

A

V

P

V

A

T

M

R

Q

N

A

A

L

M

A

S

D

D

Q

L

L

V

I

V

D

D

N

N

L

L

E

K

A

A

-

W

F

F

A

S

R

T

G

G

-

E

A

A

M

L

T

T

P

P

active site: L94 (≠ A96), R228 (= R232), D252 (= D256), E257 (= E261), H275 (= H285)
binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4r,5r)-3,4,5-trihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: V70 (≠ T72), G71 (= G73), F146 (≠ V151), G147 (= G152), L148 (≠ G153), G149 (= G154), R150 (≠ S155), I151 (≠ L156), H169 (vs. gap), T170 (≠ L172), R171 (≠ Q173), T172 (≠ A174), I198 (≠ H202), V199 (≠ C203), P200 (= P204), S204 (≠ E208), T205 (= T209), V226 (≠ T230), G227 (≠ A231), R228 (= R232)

5v7gA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
38% identity, 85% coverage: 43:315/322 of query aligns to 43:307/317 of 5v7gA

query
sites
5v7gA

A

S

S

G

I

I

A

A

I

V

V

S

N

G

K

K

L

L

P

P

I

V

S

P

G

-

A

-

L

L

M

M

A

D

R

A

L

F

P

P

Q

S

L

L

K

E

M

I

V

V

A

A

V

N

A

F

A
x
G

T
x
V

G
|
G

T

Y

N

D

N

G

V

V

D

D

L

V

E

S

A

R

A

A

R

A

R

A

Q

R

G

G

I

I

V

V

V

V

S

T

N

N

I

T

Q

P

G

D

Y

V

A
x
L

V

T

H

E

T

E

V
|
V

P

A

E

D

H

T

V

A

F

I

S

G

L

L

L

L

L

L

A

N

L

T

S

L

R

R

N

L

L

L

L

P

A

Q

Y

A

R

E

Q

Q

S

W

V

L

A

R

E

Q

G

G

R

R

W

W

Q

V

R

R

A

E

E

G

Q

A

F

F

C

P

F

L

F

-

D

-

H

S

P

P

I

L

R

-

D

S

L

L

H

R

G

G

A

R

T

T

L

V

G

G

V

L

V
x
F

G
|
G

G
x
L

G
|
G

S
x
R

L
x
I