SitesBLAST
Comparing Dsui_0258 FitnessBrowser__PS:Dsui_0258 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
44% identity, 81% coverage: 3:293/360 of query aligns to 18:318/378 of P69874
- C26 (≠ R11) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (= F12) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (≠ L30) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (= C39) mutation to T: Loss of ATPase activity and transport.
- L60 (= L45) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (≠ F61) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (= V124) mutation to M: Loss of ATPase activity and transport.
- D172 (= D161) mutation to N: Loss of ATPase activity and transport.
- C276 (≠ A252) mutation to A: Lower ATPase activity and transport efficiency.
- E297 (≠ H272) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.
8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
44% identity, 81% coverage: 3:293/360 of query aligns to 3:303/358 of 8y5iA
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
41% identity, 87% coverage: 1:314/360 of query aligns to 5:320/353 of 1vciA
2d62A Crystal structure of multiple sugar binding transport atp-binding protein
39% identity, 82% coverage: 3:296/360 of query aligns to 7:324/375 of 2d62A
3d31A Modbc from methanosarcina acetivorans (see paper)
38% identity, 91% coverage: 3:331/360 of query aligns to 2:336/348 of 3d31A
Sites not aligning to the query:
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
37% identity, 87% coverage: 2:313/360 of query aligns to 3:336/369 of P19566
- L86 (= L85) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P163) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D168) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- E306 (≠ Q293) mutation to K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
39% identity, 82% coverage: 2:296/360 of query aligns to 2:310/374 of 2awnB
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
39% identity, 82% coverage: 2:296/360 of query aligns to 2:310/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F12), S37 (= S37), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), Q81 (= Q81), R128 (= R132), A132 (≠ Q136), S134 (= S138), G136 (= G140), Q137 (= Q141), E158 (= E162), H191 (= H195)
- binding magnesium ion: S42 (≠ T42), Q81 (= Q81)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
39% identity, 82% coverage: 2:296/360 of query aligns to 2:310/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F12), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R132), S134 (= S138), Q137 (= Q141)
- binding beryllium trifluoride ion: S37 (= S37), G38 (= G38), K41 (= K41), Q81 (= Q81), S134 (= S138), G136 (= G140), H191 (= H195)
- binding magnesium ion: S42 (≠ T42), Q81 (= Q81)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
39% identity, 82% coverage: 2:296/360 of query aligns to 2:310/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F12), V17 (≠ A17), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R132), A132 (≠ Q136), S134 (= S138), Q137 (= Q141)
- binding tetrafluoroaluminate ion: S37 (= S37), G38 (= G38), K41 (= K41), Q81 (= Q81), S134 (= S138), G135 (= G139), G136 (= G140), E158 (= E162), H191 (= H195)
- binding magnesium ion: S42 (≠ T42), Q81 (= Q81)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
39% identity, 82% coverage: 2:296/360 of query aligns to 2:310/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ F12), V17 (≠ A17), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R132), A132 (≠ Q136), S134 (= S138), Q137 (= Q141)
- binding magnesium ion: S42 (≠ T42), Q81 (= Q81)
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
39% identity, 82% coverage: 2:296/360 of query aligns to 3:311/371 of P68187
- A85 (= A84) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (= K105) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V117) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ L120) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ S122) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ D127) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G140) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D161) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ P231) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- F241 (≠ V242) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- W267 (= W251) mutation to G: Normal maltose transport but constitutive mal gene expression.
- G278 (= G262) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- S282 (≠ V266) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G284 (≠ F268) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G302 (≠ A287) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- E308 (≠ Q293) mutation to Q: Maltose transport is affected but retains ability to interact with MalT.
Sites not aligning to the query:
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
1g291 Malk (see paper)
43% identity, 66% coverage: 2:239/360 of query aligns to 3:242/372 of 1g291
- binding magnesium ion: D69 (≠ E62), E71 (≠ S64), K72 (≠ E65), K79 (≠ R72), D80 (≠ E73)
- binding pyrophosphate 2-: S38 (= S37), G39 (= G38), C40 (= C39), G41 (= G40), K42 (= K41), T43 (= T42), T44 (= T43)
Sites not aligning to the query:
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
39% identity, 82% coverage: 3:296/360 of query aligns to 1:308/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ F12), S35 (= S37), G36 (= G38), C37 (= C39), G38 (= G40), K39 (= K41), S40 (≠ T42), T41 (= T43), R126 (= R132), A130 (≠ Q136), S132 (= S138), G134 (= G140), Q135 (= Q141)
8hplC Lpqy-sugabc in state 1 (see paper)
40% identity, 75% coverage: 17:285/360 of query aligns to 16:275/384 of 8hplC