SitesBLAST
Comparing Dsui_0378 FitnessBrowser__PS:Dsui_0378 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
54% identity, 97% coverage: 12:461/465 of query aligns to 5:456/456 of 8jdsA
- binding flavin-adenine dinucleotide: E32 (= E38), P68 (= P74), G70 (= G76), T71 (≠ V77), G72 (= G78), T73 (≠ S79), G74 (≠ S80), G78 (≠ H84), V79 (= V85), L90 (= L96), P132 (= P138), G133 (= G139), A134 (= A140), G140 (= G146), M141 (= M147), A143 (= A149), T144 (= T150), A146 (= A152), S147 (= S153), E200 (= E203), G201 (= G204), I206 (= I209), W323 (≠ Y328), E414 (= E419), H415 (= H420), N451 (= N456)
- binding manganese (ii) ion: H370 (= H375), H377 (= H382), E414 (= E419)
- binding pyruvic acid: R319 (= R324), H370 (= H375), H377 (= H382), H415 (= H420)
8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
54% identity, 97% coverage: 12:461/465 of query aligns to 5:455/455 of 8jdtA
- binding 2-ketobutyric acid: R318 (= R324), H369 (= H375), H376 (= H382), H414 (= H420)
- binding flavin-adenine dinucleotide: P68 (= P74), G70 (= G76), T71 (≠ V77), G72 (= G78), T73 (≠ S79), G74 (≠ S80), G78 (≠ H84), V79 (= V85), L90 (= L96), P132 (= P138), G133 (= G139), A134 (= A140), G140 (= G146), M141 (= M147), A143 (= A149), T144 (= T150), A146 (= A152), S147 (= S153), E200 (= E203), G201 (= G204), I206 (= I209), W322 (≠ Y328), E413 (= E419), H414 (= H420), N450 (= N456)
- binding manganese (ii) ion: H369 (= H375), H376 (= H382), E413 (= E419)
8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
53% identity, 97% coverage: 12:461/465 of query aligns to 5:455/455 of 8jdeA
- binding flavin-adenine dinucleotide: P68 (= P74), G70 (= G76), T71 (≠ V77), G72 (= G78), T73 (≠ S79), G74 (≠ S80), G78 (≠ H84), V79 (= V85), L90 (= L96), P132 (= P138), G133 (= G139), A134 (= A140), G140 (= G146), M141 (= M147), A143 (= A149), T144 (= T150), A146 (= A152), S147 (= S153), E200 (= E203), G201 (= G204), I206 (= I209), W322 (≠ Y328), E413 (= E419), H414 (= H420), N450 (= N456)
- binding lactic acid: R318 (= R324), H369 (= H375), H376 (= H382), H414 (= H420)
- binding manganese (ii) ion: H369 (= H375), H376 (= H382), E413 (= E419)
8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
54% identity, 97% coverage: 12:461/465 of query aligns to 5:455/455 of 8jduA
- binding 2-oxopentanoic acid: R318 (= R324), W322 (≠ Y328), H369 (= H375), H376 (= H382), H414 (= H420)
- binding flavin-adenine dinucleotide: P68 (= P74), G70 (= G76), T71 (≠ V77), G72 (= G78), T73 (≠ S79), G74 (≠ S80), G78 (≠ H84), V79 (= V85), L90 (= L96), P132 (= P138), G133 (= G139), A134 (= A140), G140 (= G146), M141 (= M147), A143 (= A149), T144 (= T150), A146 (= A152), S147 (= S153), E200 (= E203), G201 (= G204), I206 (= I209), W322 (≠ Y328), E413 (= E419), N450 (= N456)
- binding manganese (ii) ion: H369 (= H375), H376 (= H382), E413 (= E419)
8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
54% identity, 97% coverage: 12:461/465 of query aligns to 5:456/456 of 8jdrA
- binding flavin-adenine dinucleotide: P68 (= P74), G70 (= G76), T71 (≠ V77), G72 (= G78), T73 (≠ S79), G74 (≠ S80), G78 (≠ H84), V79 (= V85), L90 (= L96), P132 (= P138), G133 (= G139), A134 (= A140), G140 (= G146), M141 (= M147), A143 (= A149), T144 (= T150), A146 (= A152), S147 (= S153), E200 (= E203), G201 (= G204), I206 (= I209), Y324 (≠ F329), H370 (= H375), E414 (= E419), N451 (= N456)
- binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: R319 (= R324), W323 (≠ Y328), H415 (= H420)
8jdqA Crystal structure of h405a mldhd in complex with d-2-hydroxyisocaproic acid (see paper)
54% identity, 97% coverage: 12:461/465 of query aligns to 5:456/456 of 8jdqA
- binding (2R)-2-hydroxy-4-methylpentanoic acid: R319 (= R324), W323 (≠ Y328), H370 (= H375), H415 (= H420)
- binding flavin-adenine dinucleotide: P68 (= P74), G70 (= G76), T71 (≠ V77), G72 (= G78), T73 (≠ S79), G74 (≠ S80), G78 (≠ H84), V79 (= V85), L90 (= L96), P132 (= P138), G133 (= G139), A134 (= A140), G140 (= G146), M141 (= M147), A143 (= A149), T144 (= T150), A146 (= A152), S147 (= S153), E200 (= E203), G201 (= G204), I206 (= I209), H370 (= H375), E414 (= E419), N451 (= N456)
8jdoA Crystal structure of h405a mldhd in complex with d-2-hydroxyhexanoic acid (see paper)
54% identity, 97% coverage: 12:461/465 of query aligns to 5:456/456 of 8jdoA
- binding (2R)-2-hydroxyhexanoic acid: R319 (= R324), W323 (≠ Y328), H415 (= H420)
- binding flavin-adenine dinucleotide: P68 (= P74), G70 (= G76), T71 (≠ V77), G72 (= G78), T73 (≠ S79), G74 (≠ S80), G78 (≠ H84), V79 (= V85), L90 (= L96), P132 (= P138), G133 (= G139), A134 (= A140), G140 (= G146), M141 (= M147), A143 (= A149), T144 (= T150), A146 (= A152), S147 (= S153), E200 (= E203), G201 (= G204), I206 (= I209), Y324 (≠ F329), H370 (= H375), E414 (= E419), N451 (= N456)
8jdnA Crystal structure of h405a mldhd in complex with d-2-hydroxyvaleric acid (see paper)
54% identity, 97% coverage: 12:461/465 of query aligns to 5:456/456 of 8jdnA
- binding flavin-adenine dinucleotide: P68 (= P74), G70 (= G76), T71 (≠ V77), G72 (= G78), T73 (≠ S79), G74 (≠ S80), G78 (≠ H84), V79 (= V85), L90 (= L96), P132 (= P138), G133 (= G139), A134 (= A140), G140 (= G146), M141 (= M147), A143 (= A149), T144 (= T150), A146 (= A152), S147 (= S153), E200 (= E203), G201 (= G204), I206 (= I209), H370 (= H375), E414 (= E419), N451 (= N456)
- binding (2R)-2-oxidanylpentanoic acid: R319 (= R324), W323 (≠ Y328), H415 (= H420)
8jdgA Crystal structure of h405a mldhd in complex with d-2-hydroxybutanoic acid (see paper)
54% identity, 97% coverage: 12:461/465 of query aligns to 5:456/456 of 8jdgA
- binding flavin-adenine dinucleotide: P68 (= P74), G70 (= G76), T71 (≠ V77), G72 (= G78), T73 (≠ S79), G74 (≠ S80), G78 (≠ H84), V79 (= V85), L90 (= L96), P132 (= P138), G133 (= G139), A134 (= A140), G140 (= G146), M141 (= M147), A143 (= A149), T144 (= T150), A146 (= A152), S147 (= S153), E200 (= E203), G201 (= G204), I206 (= I209), H370 (= H375), E414 (= E419), N451 (= N456)
- binding (2R)-2-oxidanylbutanoic acid: R319 (= R324), H415 (= H420)
8jdbA Crystal structure of h405a mldhd in complex with d-2-hydroxyoctanoic acid (see paper)
54% identity, 97% coverage: 12:461/465 of query aligns to 5:456/456 of 8jdbA
- binding flavin-adenine dinucleotide: P68 (= P74), G70 (= G76), T71 (≠ V77), G72 (= G78), T73 (≠ S79), G74 (≠ S80), G78 (≠ H84), V79 (= V85), L90 (= L96), P132 (= P138), G133 (= G139), A134 (= A140), G140 (= G146), M141 (= M147), A143 (= A149), T144 (= T150), A146 (= A152), S147 (= S153), E200 (= E203), G201 (= G204), I206 (= I209), Y324 (≠ F329), H370 (= H375), E414 (= E419), N451 (= N456)
- binding (2R)-2-oxidanyloctanoic acid: V75 (= V81), R319 (= R324), W323 (≠ Y328), H415 (= H420)
8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
54% identity, 97% coverage: 12:461/465 of query aligns to 5:455/455 of 8jdxA
- binding flavin-adenine dinucleotide: E32 (= E38), P68 (= P74), G70 (= G76), T71 (≠ V77), G72 (= G78), T73 (≠ S79), G74 (≠ S80), G78 (≠ H84), V79 (= V85), L90 (= L96), P132 (= P138), G133 (= G139), A134 (= A140), G140 (= G146), M141 (= M147), A143 (= A149), T144 (= T150), A146 (= A152), S147 (= S153), E200 (= E203), G201 (= G204), I206 (= I209), W322 (≠ Y328), E413 (= E419), H414 (= H420), N450 (= N456)
- binding 3-methyl-2-oxobutanoic acid: R318 (= R324), H369 (= H375), H376 (= H382), H414 (= H420)
- binding manganese (ii) ion: H369 (= H375), H376 (= H382), E413 (= E419)
8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
53% identity, 97% coverage: 12:461/465 of query aligns to 5:454/454 of 8jdvA
- binding 2-Ketohexanoic acid: V75 (= V81), R317 (= R324), W321 (≠ Y328), H368 (= H375), H375 (= H382), H413 (= H420)
- binding flavin-adenine dinucleotide: P68 (= P74), G70 (= G76), T71 (≠ V77), G72 (= G78), T73 (≠ S79), G74 (≠ S80), G78 (≠ H84), V79 (= V85), L90 (= L96), P132 (= P138), G133 (= G139), A134 (= A140), G140 (= G146), M141 (= M147), A143 (= A149), T144 (= T150), A146 (= A152), S147 (= S153), E200 (= E203), G201 (= G204), I206 (= I209), W321 (≠ Y328), Y322 (≠ F329), E412 (= E419), H413 (= H420), N449 (= N456)
- binding manganese (ii) ion: H368 (= H375), H375 (= H382), E412 (= E419)
8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
53% identity, 97% coverage: 12:461/465 of query aligns to 5:455/455 of 8jdpA
- binding flavin-adenine dinucleotide: P68 (= P74), G70 (= G76), T71 (≠ V77), G72 (= G78), T73 (≠ S79), G74 (≠ S80), G78 (≠ H84), V79 (= V85), L90 (= L96), P132 (= P138), G133 (= G139), A134 (= A140), G140 (= G146), M141 (= M147), A143 (= A149), T144 (= T150), A146 (= A152), S147 (= S153), E200 (= E203), G201 (= G204), I206 (= I209), H369 (= H375), E413 (= E419), N450 (= N456)
- binding deaminohydroxyvaline: R319 (= R324), H414 (= H420)
8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
54% identity, 97% coverage: 12:461/465 of query aligns to 5:454/454 of 8jdzA
- binding (3S)-3-methyl-2-oxopentanoic acid: R318 (= R324), W322 (≠ Y328), H369 (= H375), H376 (= H382), H413 (= H420)
- binding flavin-adenine dinucleotide: E32 (= E38), P68 (= P74), G70 (= G76), T71 (≠ V77), G72 (= G78), T73 (≠ S79), G74 (≠ S80), G78 (≠ H84), V79 (= V85), L90 (= L96), P132 (= P138), G133 (= G139), A134 (= A140), G140 (= G146), M141 (= M147), A143 (= A149), T144 (= T150), A146 (= A152), S147 (= S153), E200 (= E203), G201 (= G204), I206 (= I209), W322 (≠ Y328), E412 (= E419), H413 (= H420), N449 (= N456)
- binding manganese (ii) ion: H369 (= H375), H376 (= H382), E412 (= E419)
8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
54% identity, 97% coverage: 12:461/465 of query aligns to 5:454/454 of 8jdyA
- binding 2-oxo-4-methylpentanoic acid: R318 (= R324), W322 (≠ Y328), S336 (≠ P342), H369 (= H375), H376 (= H382), H413 (= H420)
- binding flavin-adenine dinucleotide: P68 (= P74), G70 (= G76), T71 (≠ V77), G72 (= G78), T73 (≠ S79), G74 (≠ S80), G78 (≠ H84), V79 (= V85), L90 (= L96), P132 (= P138), G133 (= G139), A134 (= A140), G140 (= G146), M141 (= M147), A143 (= A149), T144 (= T150), A146 (= A152), S147 (= S153), E200 (= E203), G201 (= G204), I206 (= I209), E412 (= E419), N449 (= N456)
- binding manganese (ii) ion: H369 (= H375), H376 (= H382), E412 (= E419)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
32% identity, 89% coverage: 46:460/465 of query aligns to 45:463/467 of 7qh2C
- binding flavin-adenine dinucleotide: V73 (≠ P74), G75 (= G76), S76 (≠ V77), G77 (= G78), T78 (≠ S79), G79 (≠ S80), L80 (≠ V81), A83 (≠ H84), C84 (≠ V85), P137 (= P138), G138 (= G139), E139 (vs. gap), A142 (= A142), T143 (= T143), G146 (= G146), N147 (≠ M147), S149 (≠ A149), T150 (= T150), A152 (= A152), G153 (≠ S153), E203 (= E203), G204 (= G204), I209 (= I209), E422 (= E419), H423 (= H420)
- binding fe (iii) ion: H377 (= H375), H384 (= H382), E422 (= E419)
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
31% identity, 89% coverage: 53:465/465 of query aligns to 107:521/521 of Q8N465
- S109 (≠ T55) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ I73) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (≠ V77) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ S93) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (= M99) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- 169:521 (vs. 115:465, 31% identical) natural variant: Missing (in D2HGA1; uncertain significance)
- C172 (≠ V118) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (= P135) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (= A149) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ P175) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G177) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ R313) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R324) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- T390 (≠ Y328) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ C340) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- 400:521 (vs. 341:465, 30% identical) natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
- K401 (≠ P342) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- R419 (≠ N360) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ S367) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H375) binding Zn(2+); mutation to A: Loss of catalytic activity.
- G436 (= G377) to V: slight reduction in catalytic activity
- N439 (= N380) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H382) binding Zn(2+); mutation to A: Loss of catalytic activity.
- N443 (≠ V384) binding (R)-2-hydroxyglutarate; mutation to A: Significantly reduced catalytic activity.
- V444 (= V385) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (≠ V387) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E419) binding Zn(2+); mutation to A: Loss of catalytic activity.
- H476 (= H420) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- G477 (= G421) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
31% identity, 88% coverage: 53:462/465 of query aligns to 54:465/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R324), T337 (≠ Y328), K348 (≠ P342), Y379 (≠ F373), H381 (= H375), H388 (= H382), H423 (= H420)
- binding flavin-adenine dinucleotide: P75 (= P74), Q76 (≠ Y75), G77 (= G76), G78 (≠ V77), N79 (≠ G78), T80 (≠ S79), G81 (≠ S80), M82 (≠ V81), G85 (≠ H84), S86 (≠ V85), L139 (≠ P138), G140 (= G139), A141 (= A140), C145 (vs. gap), G149 (= G146), N150 (≠ M147), A152 (= A149), T153 (= T150), G157 (= G154), G207 (= G204), I212 (= I209), E422 (= E419), N459 (= N456)
- binding zinc ion: H381 (= H375), H388 (= H382), E422 (= E419)
Sites not aligning to the query:
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
31% identity, 88% coverage: 53:462/465 of query aligns to 54:465/466 of 6lpuA
- binding flavin-adenine dinucleotide: P75 (= P74), G77 (= G76), G78 (≠ V77), N79 (≠ G78), T80 (≠ S79), G81 (≠ S80), G85 (≠ H84), S86 (≠ V85), L139 (≠ P138), G140 (= G139), A141 (= A140), C145 (vs. gap), H146 (vs. gap), G148 (= G145), G149 (= G146), N150 (≠ M147), A152 (= A149), T153 (= T150), A155 (= A152), E206 (= E203), G207 (= G204), I211 (= I208), I212 (= I209), E422 (= E419), N459 (= N456)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R324), T337 (≠ Y328), K348 (≠ P342), Y379 (≠ F373), H381 (= H375), H388 (= H382), H423 (= H420)
- binding zinc ion: H381 (= H375), H388 (= H382), E422 (= E419)
Sites not aligning to the query:
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
31% identity, 88% coverage: 53:462/465 of query aligns to 54:465/466 of 6lpqA
- binding flavin-adenine dinucleotide: P75 (= P74), G77 (= G76), G78 (≠ V77), N79 (≠ G78), T80 (≠ S79), G81 (≠ S80), G85 (≠ H84), S86 (≠ V85), L139 (≠ P138), G140 (= G139), A141 (= A140), C145 (vs. gap), H146 (vs. gap), G149 (= G146), N150 (≠ M147), A152 (= A149), T153 (= T150), A155 (= A152), G157 (= G154), E206 (= E203), G207 (= G204), I211 (= I208), I212 (= I209), E422 (= E419), N459 (= N456)
- binding d-malate: M82 (≠ V81), R333 (= R324), T337 (≠ Y328), K348 (≠ P342), Y379 (≠ F373), H381 (= H375), H388 (= H382), E422 (= E419), H423 (= H420)
- binding zinc ion: H381 (= H375), H388 (= H382), E422 (= E419)
Sites not aligning to the query:
Query Sequence
>Dsui_0378 FitnessBrowser__PS:Dsui_0378
MTTASAPLPAALVEALRQQFGTRYSQGESVRLQHGRDESKHEPMLPDGVVFAESTEEVAA
VVRLCREYRVPVIPYGVGSSVEGHVLAVHGGISIDLSGMNRVLSIHAEDLDVTVQAGVTR
KQLNDELKGTGLFFPIDPGADATLGGMAATRASGTNAVRYGTMRENVLSLTVVTPEGKIV
RTGSRARKSSAGYDLTRLFVGSEGTLGIITELTVKLYPIPEAMSAAVCAFPSVDAAVDTV
IQTIQLGVPVARVELLDALTLQAINRYSKTTLPEAPTLFFEFHGSDSGVQEQAELTQEIA
QGNGGQDFQWATRQEDRSRLWQARHDAYFACLQLKPGARCFPTDVCVPISRLAECIHATN
EDIAQVSLPIALFGHVGDGNFHLVVLVDTDNPKEMAEGEWISQRVVERAIAMEGTCTGEH
GIGLGKQHYLLQEHGEDGVALMRTLKTALDPLNLMNPGKILPPQA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory