SitesBLAST

7kn1A Crystal structure of udp-glucose-4-epimerase (gale) from stenotrophomonas maltophila with bound NAD and formylated udp- arabinopyranose
57% identity, 98% coverage: 1:329/337 of query aligns to 3:331/336 of 7kn1A

query
sites
7kn1A

M

M

H

N

I

V

L

L

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V

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T

G
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G

G

G

L

A

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G

Y
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I

G

G

S

S

H

H

T

A

C

C

V

V

E

E

L

L

L

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Q

Q

A

Q

G

G

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H

G

G

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V

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V

I

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D

N
x
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L
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L

S
x
C

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N

S
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S

R

D

A

A

D

S

V

V

A

V

E

E

R

R

I

I

A

G

G

R

L

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G

G

A

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A

A

P

P

I

V

F

F

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x
A

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D

V
x
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M

C

A

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M

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D

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F

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A

G
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A

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V

G

G

E

E

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S

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A

K

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P

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Y

Y

Y

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E

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N

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S

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L

L

F

L

E

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A

A

M

M

A

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D

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A

G

G

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T

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L

V

V

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F

S
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S

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A

T
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T

V

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Y

Y

G

G

D

N

P

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H

D

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C

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P

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E

E

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Y

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G

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L

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G

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E

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G

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x
S

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N

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L

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Q

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G

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x
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G

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D

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Y

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Y

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M

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N

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L

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R

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A

D

D

P

A

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L

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A

A

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E

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L

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G

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D

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R

active site: S126 (= S124), Y150 (= Y148), K154 (= K152)
binding nicotinamide-adenine-dinucleotide: G9 (= G7), G12 (= G10), Y13 (= Y11), I14 (= I12), D33 (= D31), S34 (≠ N32), L35 (= L33), C36 (≠ S34), N37 (= N35), S38 (= S36), A59 (≠ G57), D60 (= D58), I61 (≠ V59), R62 (= R60), F82 (= F80), A83 (= A81), A84 (≠ G82), K86 (= K84), S124 (= S122), S125 (= S123), S126 (= S124), Y150 (= Y148), K154 (= K152), Y178 (= Y176), P181 (= P179)
binding UDP-4-deoxy-4-formamido-beta-L-arabinopyranose: V88 (= V86), S126 (= S124), T128 (= T126), Y150 (= Y148), N180 (= N178), S199 (≠ N197), N200 (= N198), L201 (= L199), Y204 (= Y202), Q217 (≠ S215), V218 (= V216), F219 (= F217), G230 (= G228), R232 (= R230), Y234 (≠ F232), V270 (= V268), R293 (= R291), D296 (= D294)

2udpA Udp-galactose 4-epimerase complexed with udp-phenol (see paper)
57% identity, 97% coverage: 1:328/337 of query aligns to 1:329/338 of 2udpA

query
sites
2udpA

M

M

H

R

I

V

L

L

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V

T

T

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G

G

G

L
x
S

G
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G

Y
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Y

I
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I

G

G

S

S

H

H

T

T

C

C

V

V

E

Q

L

L

Q

Q

A

N

G

G

H

H

G

D

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V

T

I

I

I

L

L

D
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D

N
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N

L
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L

S
x
C

N
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N

S
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S

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A

R

D

S

V

V

A

L

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I

A

E

G

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L

G

G

A

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F

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E

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G

D
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D

V
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A
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A

G
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A

A

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G

G

E

E

S

S

V

V

A

Q

Q

K

P

P

L

L

R

E

Y

Y

E

D

C
x
N

N

N

V

V

G

N

G

G

S

T

L

L

V

R

L

L

F

I

E

S

A

A

M

M

A

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E

A

A

A

G

N

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K

T

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L

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A
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Y

G

G

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D

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K

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-

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Y

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V

V

G

G

A

A

H

H

E

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S

G

G

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M

G

G

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N

L
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L

M

M

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G

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G

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N

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Y

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T

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I

I

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M

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G

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Y

W

A

A

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D

P

A

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K

A

A

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active site: S124 (= S124), A125 (= A125), T126 (= T126), Y149 (= Y148), K153 (= K152), M189 (≠ L188)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), S9 (≠ L9), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), L33 (= L33), C34 (≠ S34), N35 (= N35), S36 (= S36), G57 (= G57), D58 (= D58), I59 (≠ V59), R60 (= R60), F80 (= F80), A81 (= A81), G82 (= G82), K84 (= K84), N99 (≠ C99), S122 (= S122), S123 (= S123), S124 (= S124), Y149 (= Y148), K153 (= K152), Y177 (= Y176), F178 (= F177), P180 (= P179)
binding phenyl-uridine-5'-diphosphate: K84 (= K84), V86 (= V86), Y149 (= Y148), N179 (= N178), N198 (= N197), N199 (= N198), L200 (= L199), Y203 (= Y202), L215 (= L214), A216 (≠ S215), I217 (≠ V216), F218 (= F217), G229 (= G228), R231 (= R230), Y233 (≠ F232), V269 (= V268), R292 (= R291), D295 (= D294)

1udcA Structure of udp-galactose-4-epimerase complexed with udp-mannose (see paper)
57% identity, 97% coverage: 1:328/337 of query aligns to 1:329/338 of 1udcA

query
sites
1udcA

M

M

H

R

I

V

L

L

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V

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T

G
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G

G

G

L
x
S

G
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G

Y
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Y

I
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I

G

G

S

S

H

H

T

T

C

C

V

V

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Q

L

L

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Q

A

N

G

G

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H

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T

I

I

I

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L

D
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D

N
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N

L
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L

S
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C

N
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N

S
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S

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K

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R

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S

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V

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I

I

A

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L

G

G

A

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F

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E

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D
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D

V
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R

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N

R

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A

L

L

L

M

C

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R

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I

I

F

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A

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D

A

T

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V

I

I

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H

F
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F

A
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A

G
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G

L

L

K
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K

A

A

V

V

G

G

E

E

S

S

V

V

A

Q

Q

K

P

P

L

L

R

E

Y

Y

E

D

C
x
N

N

N

V

V

G

N

G

G

S

T

L

L

V

R

L

L

F

I

E

S

A

A

M

M

A

R

E

A

A

A

G

N

V

V

K

K

T

N

L

F

V

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F

F

S
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S

A
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A

T
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T

V

V

Y

Y

G

G

D

D

P

Q

H

P

A

K

V

I

P

P

I

Y

R

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E

E

D

S

F

F

P

P

L

T

-

G

R

T

A

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T

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N

S

P

P

Y
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Y

G

G

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K

S

S

K
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K

L

L

M

M

L

V

E

E

E

Q

V

I

L

L

A

T

D

D

M

L

A

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A

K

A

A

D

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S

P

Q

D

W

W

R

S

I

I

A

A

R

L

L

L

R

R

Y
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Y

F
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F

N
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N

P
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P

V

V

G

G

A

A

H

H

E

P

S

S

G

G

L

D

L
x
M

G

G

E

E

A

D

P

P

N

Q

G

G

I

I

P

P

N
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N

L
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L

M

M

P

P

Y

Y

V

I

A

A

Q

Q

V

V

A

A

A

V

G

G

L

R

R

R

A

D

R

S

L
|
L

S
x
A

V
x
I

F
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F

G

G

N

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

G
|
G

V

V

R
|
R

D

D

F
x
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

Q

D

G

G

H

H

L

V

A

V

A

A

L

M

A

E

A

K

L

L

A

A

R

N

G

K

E

P

G

G

M

V

L

H

T

I

V

Y

N

N

L

L

G

G

T

A

G

G

R

V

G

G

Y

N

S

S

V
|
V

L

L

D

D

L

V

V

V

R

N

A

A

F

F

E

S

A

K

A

A

S

C

G

G

R

K

T

P

I

V

P

N

F

Y

D

H

F

F

V

A

P

P

R

R

R
|
R

P

E

G

G

D
|
D

I

L

A

P

A

A

C
x
Y

Y

W

A

A

D

D

P

A

A

S

L

K

A

A

A

D

S

R

L

E

L

L

G

N

W

W

T

R

A

V

R

T

R

R

D

T

L

L

A

D

A

E

M

M

C

A

R

Q

D

D

T

T

W

W

R

H

W

W

Q

Q

active site: S124 (= S124), A125 (= A125), T126 (= T126), Y149 (= Y148), K153 (= K152), M189 (≠ L188)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), S9 (≠ L9), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), L33 (= L33), C34 (≠ S34), N35 (= N35), S36 (= S36), G57 (= G57), D58 (= D58), I59 (≠ V59), R60 (= R60), F80 (= F80), A81 (= A81), G82 (= G82), K84 (= K84), N99 (≠ C99), S122 (= S122), S123 (= S123), S124 (= S124), Y149 (= Y148), K153 (= K152), Y177 (= Y176), P180 (= P179)
binding uridine-5'-diphosphate-mannose: T126 (= T126), Y149 (= Y148), F178 (= F177), N179 (= N178), N198 (= N197), N199 (= N198), L200 (= L199), L215 (= L214), A216 (≠ S215), I217 (≠ V216), F218 (= F217), G229 (= G228), R231 (= R230), Y233 (≠ F232), V269 (= V268), R292 (= R291), D295 (= D294), Y299 (≠ C298)

P09147 UDP-glucose 4-epimerase; Galactowaldenase; UDP-galactose 4-epimerase; EC 5.1.3.2 from Escherichia coli (strain K12) (see 10 papers)
57% identity, 97% coverage: 1:328/337 of query aligns to 1:329/338 of P09147

query
sites
P09147

M

M

H

R

I

V

L

L

V

V

T

T

G

G

L

S

G

G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

E

Q

L

L

Q

Q

A

N

G

G

H

H

G

D

V

V

T

I

I

I

L

L

D
|
D

N
|
N

L
|
L

S
x
C

N
|
N

S
|
S

R

K

A

R

D

S

V

V

A

L

E

P

R

V

I

I

A

E

G

R

L

L

G

G

A

K

A

H

P

P

I

T

F

F

I

V

E

E

G

G

D
|
D

V
x
I

R

R

D

N

R

E

Q

A

L

L

L

M

C

T

R

E

I

I

F

L

R

H

E

D

Q

H

T

A

V

I

D

D

A

T

V

V

I

I

H

H

F
|
F

A
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A

G
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G

L
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L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

A

Q

Q

K

P

P

L

L

R

E

Y

Y

E

D

C
x
N

N

N

V

V

G

N

G

G

S

T

L

L

V

R

L

L

F

I

E

S

A

A

M

M

A

R

E

A

A

A

G

N

V

V

K

K

T

N

L

F

V

I

F

F

S

S

S
|
S

A

A

T

T

V

V

Y

Y

G

G

D

D

P

Q

H

P

A

K

V

I

P

P

I

Y

R

V

E

E

D

S

F

F

P

P

L

T

-

G

R

T

A

P

T

Q

N

S

P

P

Y
|
Y

G

G

R

K

S

S

K
|
K

L

L

M

M

L

V

E

E

E

Q

V

I

L

L

A

T

D

D

M

L

A

Q

A

K

A

A

D

Q

S

P

Q

D

W

W

R

S

I

I

A

A

R

L

L

L

R

R

Y

Y

F
|
F

N

N

P

P

V

V

G

G

A

A

H

H

E

P

S

S

G

G

L

D

L

M

G

G

E

E

A

D

P

P

N

Q

G

G

I

I

P

P

N

N

L

L

M

M

P

P

Y

Y

V

I

A

A

Q

Q

V

V

A

A

A

V

G

G

L

R

R

R

A

D

R

S

L

L

S

A

V

I

F

F

G

G

N

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

G

G

V

V

R

R

D

D

F

Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

Q

D

G

G

H

H

L

V

A

V

A

A

L

M

A

E

A

K

L

L

A

A

R

N

G

K

E

P

G

G

M

V

L

H

T

I

V

Y

N

N

L

L

G

G

T

A

G

G

R

V

G

G

Y

N

S

S

V

V

L

L

D

D

L

V

V

V

R

N

A

A

F

F

E

S

A

K

A

A

S

C

G

G

R

K

T

P

I

V

P

N

F

Y

D

H

F

F

V

A

P

P

R

R

P

E

G

G

D

D

I

L

A

P

A

A

C
x
Y

Y

W

A

A

D

D

P

A

A

S

L

K

A

A

A

D

S

R

L

E

L

L

G

N

W

W

T

R

A

V

R

T

R

R

D

T

L

L

A

D

A

E

M

M

C

A

R

Q

D

D

T

T

W

W

R

H

W

W

Q

Q

YI 11:12 (= YI 11:12) binding
DNLCNS 31:36 (≠ DNLSNS 31:36) binding
DI 58:59 (≠ DV 58:59) binding
FAGLK 80:84 (= FAGLK 80:84) binding
N99 (≠ C99) binding
S124 (= S124) binding ; mutation to A: No major structural changes. Catalytic efficiency is very low and affinity binding is 21% of the wild-type enzyme.; mutation to T: No major structural changes. Catalytic efficiency is about 30% of that of the wild-type enzyme, and affinity binding is similar to that of the native enzyme.
Y149 (= Y148) active site, Proton acceptor; binding ; mutation to F: No major structural changes. Catalytic efficiency is very low and affinity binding is 12% of the wild-type enzyme.
K153 (= K152) binding ; mutation to A: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.; mutation to M: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.
F178 (= F177) binding
Y299 (≠ C298) mutation to C: Loss of epimerase activity with UDP-Gal by almost 5-fold, but it results in a gain of epimerase activity with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) by 230-fold with minimal changes in its three-dimensional structure.

1udaA Structure of udp-galactose-4-epimerase complexed with udp-4-deoxy-4- fluoro-alpha-d-galactose (see paper)
57% identity, 97% coverage: 1:328/337 of query aligns to 1:329/338 of 1udaA

query
sites
1udaA

M

M

H

R

I

V

L

L

V

V

T

T

G
|
G

G

G

L
x
S

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

E

Q

L

L

Q

Q

A

N

G

G

H

H

G

D

V

V

T

I

I

I

L

L

D
|
D

N
|
N

L
|
L

S
x
C

N
|
N

S
|
S

R

K

A

R

D

S

V

V

A

L

E

P

R

V

I

I

A

E

G

R

L

L

G

G

A

K

A

H

P

P

I

T

F

F

I

V

E

E

G
|
G

D
|
D

V
x
I

R
|
R

D

N

R

E

Q

A

L

L

L

M

C

T

R

E

I

I

F

L

R

H

E

D

Q

H

T

A

V

I

D

D

A

T

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V
|
V

G

G

E

E

S

S

V

V

A

Q

Q

K

P

P

L

L

R

E

Y

Y

E

D

C
x
N

N

N

V

V

G

N

G

G

S

T

L

L

V

R

L

L

F

I

E

S

A

A

M

M

A

R

E

A

A

A

G

N

V

V

K

K

T

N

L

F

V

I

F

F

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

D

P

N

H

P

A

K

V

I

P

P

I

Y

R

V

E

E

D

S

F

F

P

P

L

T

-

G

R

T

A

P

T

Q

N

S

P

P

Y
|
Y

G

G

R

K

S

S

K
|
K

L

L

M

M

L

V

E

E

E

Q

V

I

L

L

A

T

D

D

M

L

A

Q

A

K

A

A

D

Q

S

P

Q

D

W

W

R

S

I

I

A

A

R

L

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

E

P

S

S

G

G

L

D

L
x
M

G

G

E

E

A

D

P

P

N

Q

G

G

I

I

P

P

N
|
N

L
|
L

M

M

P

P

Y
|
Y

V

I

A

A

Q

Q

V

V

A

A

A

V

G

G

L

R

R

R

A

D

R

S

L
|
L

S
x
A

V
x
I

F
|
F

G

G

N

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

G
|
G

V

V

R
|
R

D

D

F
x
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

Q

D

G

G

H

H

L

V

A

V

A

A

L

M

A

E

A

K

L

L

A

A

R

N

G

K

E

P

G

G

M

V

L

H

T

I

V

Y

N

N

L

L

G

G

T

A

G

G

R

V

G

G

Y

N

S

S

V
|
V

L

L

D

D

L

V

V

V

R

N

A

A

F

F

E

S

A

K

A

A

S

C

G

G

R

K

T

P

I

V

P

N

F

Y

D

H

F

F

V

A

P

P

R

R

R
|
R

P

E

G

G

D
|
D

I

L

A

P

A

A

C
x
Y

Y

W

A

A

D

D

P

A

A

S

L

K

A

A

A

D

S

R

L

E

L

L

G

N

W

W

T

R

A

V

R

T

R

R

D

T

L

L

A

D

A

E

M

M

C

A

R

Q

D

D

T

T

W

W

R

H

W

W

Q

Q

active site: S124 (= S124), A125 (= A125), T126 (= T126), Y149 (= Y148), K153 (= K152), M189 (≠ L188)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), S9 (≠ L9), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), L33 (= L33), C34 (≠ S34), N35 (= N35), S36 (= S36), G57 (= G57), D58 (= D58), I59 (≠ V59), R60 (= R60), F80 (= F80), A81 (= A81), G82 (= G82), K84 (= K84), N99 (≠ C99), S122 (= S122), S123 (= S123), S124 (= S124), Y149 (= Y148), K153 (= K152), Y177 (= Y176), P180 (= P179)
binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: V86 (= V86), S124 (= S124), T126 (= T126), F178 (= F177), N179 (= N178), N198 (= N197), N199 (= N198), L200 (= L199), Y203 (= Y202), L215 (= L214), A216 (≠ S215), I217 (≠ V216), F218 (= F217), G229 (= G228), R231 (= R230), Y233 (≠ F232), V269 (= V268), R292 (= R291), D295 (= D294), Y299 (≠ C298)

1naiA Udp-galactose 4-epimerase from escherichia coli, oxidized (see paper)
57% identity, 97% coverage: 1:328/337 of query aligns to 1:329/338 of 1naiA

query
sites
1naiA

M

M

H

R

I

V

L

L

V

V

T

T

G
|
G

G

G

L
x
S

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

E

Q

L

L

Q

Q

A

N

G

G

H

H

G

D

V

V

T

I

I

I

L

L

D
|
D

N
|
N

L
|
L

S
x
C

N
|
N

S
|
S

R

K

A

R

D

S

V

V

A

L

E

P

R

V

I

I

A

E

G

R

L

L

G

G

A

K

A

H

P

P

I

T

F

F

I

V

E

E

G
|
G

D
|
D

V
x
I

R
|
R

D

N

R

E

Q

A

L

L

L

M

C

T

R

E

I

I

F

L

R

H

E

D

Q

H

T

A

V

I

D

D

A

T

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L
|
L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

A

Q

Q

K

P

P

L

L

R

E

Y

Y

E

D

C
x
N

N

N

V

V

G

N

G

G

S

T

L

L

V

R

L

L

F

I

E

S

A

A

M

M

A

R

E

A

A

A

G

N

V

V

K

K

T

N

L

F

V

I

F

F

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

D

P

N

H

P

A

K

V

I

P

P

I

Y

R

V

E

E

D

S

F

F

P

P

L

T

-

G

R

T

A

P

T

Q

N

S

P

P

Y
|
Y

G

G

R

K

S

S

K
|
K

L

L

M

M

L

V

E

E

E

Q

V

I

L

L

A

T

D

D

M

L

A

Q

A

K

A

A

D

Q

S

P

Q

D

W

W

R

S

I

I

A

A

R

L

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

E

P

S

S

G

G

L

D

L
x
M

G

G

E
|
E

A
x
D

P
|
P

N

Q

G

G

I

I

P

P

N
|
N

L
|
L

M

M

P

P

Y

Y

V

I

A

A

Q

Q

V

V

A

A

A

V

G

G

L

R

R

R

A

D

R

S

L
|
L

S
x
A

V
x
I

F

F

G

G

N

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

G

G

V

V

R
|
R

D

D

F
x
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

Q

D

G

G

H

H

L

V

A

V

A

A

L

M

A

E

A

K

L

L

A

A

R

N

G

K

E

P

G

G

M

V

L

H

T

I

V

Y

N

N

L

L

G

G

T

A

G

G

R

V

G

G

Y

N

S

S

V
|
V

L

L

D

D

L

V

V

V

R

N

A

A

F

F

E

S

A

K

A

A

S

C

G

G

R

K

T

P

I

V

P

N

F

Y

D

H

F

F

V

A

P

P

R

R

R
|
R

P

E

G

G

D
|
D

I

L

A

P

A

A

C
x
Y

Y

W

A

A

D

D

P

A

A

S

L

K

A

A

A

D

S

R

L

E

L

L

G

N

W

W

T

R

A

V

R

T

R

R

D

T

L

L

A

D

A

E

M

M

C

A

R

Q

D

D

T

T

W

W

R

H

W

W

Q

Q

active site: S124 (= S124), A125 (= A125), T126 (= T126), Y149 (= Y148), K153 (= K152), M189 (≠ L188)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), S9 (≠ L9), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), L33 (= L33), C34 (≠ S34), N35 (= N35), S36 (= S36), G57 (= G57), D58 (= D58), I59 (≠ V59), R60 (= R60), F80 (= F80), A81 (= A81), G82 (= G82), L83 (= L83), K84 (= K84), N99 (≠ C99), S122 (= S122), S123 (= S123), S124 (= S124), Y149 (= Y148), K153 (= K152), Y177 (= Y176), F178 (= F177), P180 (= P179)
binding 1,3-propandiol: N35 (= N35), K84 (= K84), E191 (= E190), D192 (≠ A191), P193 (= P192)
binding uridine-5'-diphosphate: N179 (= N178), N198 (= N197), N199 (= N198), L200 (= L199), L215 (= L214), A216 (≠ S215), I217 (≠ V216), R231 (= R230), Y233 (≠ F232), V269 (= V268), R292 (= R291), D295 (= D294), Y299 (≠ C298)

1lrjA Crystal structure of e. Coli udp-galactose 4-epimerase complexed with udp-n-acetylglucosamine (see paper)
57% identity, 97% coverage: 1:328/337 of query aligns to 1:329/338 of 1lrjA

query
sites
1lrjA

M

M

H

R

I

V

L

L

V

V

T

T

G
|
G

G

G

L
x
S

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

E

Q

L

L

Q

Q

A

N

G

G

H

H

G

D

V

V

T

I

I

I

L

L

D
|
D

N
|
N

L
|
L

S
x
C

N
|
N

S
|
S

R

K

A

R

D

S

V

V

A

L

E

P

R

V

I

I

A

E

G

R

L

L

G

G

A

K

A

H

P

P

I

T

F

F

I

V

E

E

G
|
G

D
|
D

V
x
I

R
|
R

D

N

R

E

Q

A

L

L

L

M

C

T

R

E

I

I

F

L

R

H

E

D

Q

H

T

A

V

I

D

D

A

T

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L
|
L

K
|
K

A

A

V
|
V

G

G

E

E

S

S

V

V

A

Q

Q

K

P

P

L

L

R

E

Y

Y

E

D

C
x
N

N

N

V

V

G

N

G

G

S

T

L

L

V

R

L

L

F

I

E

S

A

A

M

M

A

R

E

A

A

A

G

N

V

V

K

K

T

N

L

F

V

I

F

F

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

D

P

N

H

P

A

K

V

I

P

P

I

Y

R

V

E

E

D

S

F

F

P

P

L

T

-

G

R

T

A

P

T

Q

N

S

P

P

Y
|
Y

G

G

R

K

S

S

K
|
K

L

L

M

M

L

V

E

E

E

Q

V

I

L

L

A

T

D

D

M

L

A

Q

A

K

A

A

D

Q

S

P

Q

D

W

W

R

S

I

I

A

A

R

L

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

E

P

S

S

G

G

L

D

L
x
M

G

G

E

E

A

D

P

P

N

Q

G

G

I

I

P

P

N
|
N

L
|
L

M

M

P

P

Y
|
Y

V

I

A

A

Q

Q

V

V

A

A

A

V

G

G

L

R

R

R

A

D

R

S

L
|
L

S
x
A

V
x
I

F
|
F

G

G

N

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

G
|
G

V

V

R
|
R

D

D

F
x
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

Q

D

G

G

H

H

L

V

A

V

A

A

L

M

A

E

A

K

L

L

A

A

R

N

G

K

E

P

G

G

M

V

L

H

T

I

V

Y

N

N

L

L

G

G

T

A

G

G

R

V

G

G

Y

N

S

S

V
|
V

L

L

D

D

L

V

V

V

R

N

A

A

F

F

E

S

A

K

A

A

S

C

G

G

R

K

T

P

I

V

P

N

F

Y

D

H

F

F

V

A

P

P

R

R

R
|
R

P

E

G

G

D
|
D

I

L

A

P

A

A

C
x
Y

Y

W

A

A

D

D

P

A

A

S

L

K

A

A

A

D

S

R

L

E

L

L

G

N

W

W

T

R

A

V

R

T

R

R

D

T

L

L

A

D

A

E

M

M

C

A

R

Q

D

D

T

T

W

W

R

H

W

W

Q

Q

active site: S124 (= S124), A125 (= A125), T126 (= T126), Y149 (= Y148), K153 (= K152), M189 (≠ L188)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), S9 (≠ L9), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), L33 (= L33), C34 (≠ S34), N35 (= N35), S36 (= S36), G57 (= G57), D58 (= D58), I59 (≠ V59), R60 (= R60), F80 (= F80), A81 (= A81), G82 (= G82), L83 (= L83), K84 (= K84), N99 (≠ C99), S122 (= S122), S123 (= S123), S124 (= S124), Y149 (= Y148), K153 (= K152), Y177 (= Y176), F178 (= F177), P180 (= P179), N199 (= N198)
binding uridine-diphosphate-n-acetylglucosamine: K84 (= K84), V86 (= V86), S124 (= S124), T126 (= T126), Y149 (= Y148), Y177 (= Y176), F178 (= F177), N179 (= N178), N198 (= N197), N199 (= N198), L200 (= L199), Y203 (= Y202), L215 (= L214), A216 (≠ S215), I217 (≠ V216), F218 (= F217), G229 (= G228), R231 (= R230), Y233 (≠ F232), V269 (= V268), R292 (= R291), D295 (= D294), Y299 (≠ C298)

1kvrA Udp-galactose 4-epimerase complexed with udp-phenol (see paper)
57% identity, 97% coverage: 1:328/337 of query aligns to 1:329/338 of 1kvrA

query
sites
1kvrA

M

M

H

R

I

V

L

L

V

V

T

T

G
|
G

G

G

L
x
S

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

E

Q

L

L

Q

Q

A

N

G

G

H

H

G

D

V

V

T

I

I

I

L

L

D
|
D

N
|
N

L
|
L

S
x
C

N
|
N

S
|
S

R

K

A

R

D

S

V

V

A

L

E

P

R

V

I

I

A

E

G

R

L

L

G

G

A

K

A

H

P

P

I

T

F

F

I

V

E

E

G
|
G

D
|
D

V
x
I

R
|
R

D

N

R

E

Q

A

L

L

L

M

C

T

R

E

I

I

F

L

R

H

E

D

Q

H

T

A

V

I

D

D

A

T

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L
|
L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

A

Q

Q

K

P

P

L

L

R

E

Y

Y

E

D

C
x
N

N

N

V

V

G

N

G

G

S

T

L

L

V

R

L

L

F

I

E

S

A

A

M

M

A

R

E

A

A

A

G

N

V

V

K

K

T

N

L

F

V

I

F

F

S
|
S

S
x
A

A
|
A

T
|
T

V

V

Y

Y

G

G

D

D

P

Q

H

P

A

K

V

I

P

P

I

Y

R

V

E

E

D

S

F

F

P

P

L

T

-

G

R

T

A

P

T

Q

N

S

P

P

Y
|
Y

G

G

R

K

S

S

K
|
K

L

L

M

M

L

V

E

E

E

Q

V

I

L

L

A

T

D

D

M

L

A

Q

A

K

A

A

D

Q

S

P

Q

D

W

W

R

S

I

I

A

A

R

L

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

E

P

S

S

G

G

L

D

L
x
M

G

G

E

E

A

D

P

P

N

Q

G

G

I

I

P

P

N
|
N

L
|
L

M

M

P

P

Y

Y

V

I

A

A

Q

Q

V

V

A

A

A

V

G

G

L

R

R

R

A

D

R

S

L
|
L

S
x
A

V
x
I

F
|
F

G

G

N

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

G
|
G

V

V

R
|
R

D

D

F
x
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

Q

D

G

G

H

H

L

V

A

V

A

A

L

M

A

E

A

K

L

L

A

A

R

N

G

K

E

P

G

G

M

V

L

H

T

I

V

Y

N

N

L

L

G

G

T

A

G

G

R

V

G

G

Y

N

S

S

V
|
V

L

L

D

D

L

V

V

V

R

N

A

A

F

F

E

S

A

K

A

A

S

C

G

G

R

K

T

P

I

V

P

N

F

Y

D

H

F

F

V

A

P

P

R

R

R
|
R

P

E

G

G

D
|
D

I

L

A

P

A

A

C
x
Y

Y

W

A

A

D

D

P

A

A

S

L

K

A

A

A

D

S

R

L

E

L

L

G

N

W

W

T

R

A

V

R

T

R

R

D

T

L

L

A

D

A

E

M

M

C

A

R

Q

D

D

T

T

W

W

R

H

W

W

Q

Q

active site: A124 (≠ S124), A125 (= A125), T126 (= T126), Y149 (= Y148), K153 (= K152), M189 (≠ L188)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), S9 (≠ L9), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), L33 (= L33), C34 (≠ S34), N35 (= N35), S36 (= S36), G57 (= G57), D58 (= D58), I59 (≠ V59), R60 (= R60), F80 (= F80), A81 (= A81), G82 (= G82), L83 (= L83), K84 (= K84), N99 (≠ C99), S122 (= S122), S123 (= S123), A124 (≠ S124), Y149 (= Y148), K153 (= K152), Y177 (= Y176), P180 (= P179)
binding uridine-5'-diphosphate: N179 (= N178), N198 (= N197), N199 (= N198), L200 (= L199), L215 (= L214), A216 (≠ S215), I217 (≠ V216), F218 (= F217), G229 (= G228), R231 (= R230), Y233 (≠ F232), V269 (= V268), R292 (= R291), D295 (= D294), Y299 (≠ C298)

1a9yA Udp-galactose 4-epimerase mutant s124a/y149f complexed with udp- glucose (see paper)
57% identity, 97% coverage: 1:328/337 of query aligns to 1:329/338 of 1a9yA

query
sites
1a9yA

M

M

H

R

I

V

L

L

V

V

T

T

G
|
G

G

G

L
x
S

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

C

V

V

E

Q

L

L

Q

Q

A

N

G

G

H

H

G

D

V

V

T

I

I

I

L

L

D
|
D

N
|
N

L
|
L

S
x
C

N
|
N

S
|
S

R

K

A

R

D

S

V

V

A

L

E

P

R

V

I

I

A

E

G

R

L

L

G

G

A

K

A

H

P

P

I

T

F

F

I

V

E

E

G
|
G

D
|
D

V
x
I

R
|
R

D

N

R

E

Q

A

L

L

L

M

C

T

R

E

I

I

F

L

R

H

E

D

Q

H

T

A

V

I

D

D

A

T

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L
|
L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

A

Q

Q

K

P

P

L

L

R

E

Y

Y

E

D

C
x
N

N

N

V

V

G

N

G

G

S

T

L

L

V

R

L

L

F

I

E

S

A

A

M

M

A

R

E

A

A

A

G

N

V

V

K

K

T

N

L

F

V

I

F

F

S
|
S

S
x
A

A
|
A

T
|
T

V

V

Y

Y

G

G

D

D

P

Q

H

P

A

K

V

I

P

P

I

Y

R

V

E

E

D

S

F

F

P

P

L

T

-

G

R

T

A

P

T

Q

N

S

P

P

Y
x
F

G

G

R

K

S

S

K
|
K

L

L

M

M

L

V

E

E

E

Q

V

I

L

L

A

T

D

D

M

L

A

Q

A

K

A

A

D

Q

S

P

Q

D

W

W

R

S

I

I

A

A

R

L

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

E

P

S

S

G

G

L

D

L

M

G

G

E

E

A

D

P

P

N

Q

G

G

I

I

P

P

N
|
N

L
|
L

M

M

P

P

Y
|
Y

V

I

A

A

Q

Q

V

V

A

A

A

V

G

G

L

R

R

R

A

D

R

S

L
|
L

S
x
A

V
x
I

F
|
F

G

G

N

N

D

D

Y

Y

P

P

T

T

P

E

D

D

G

G

T

T

G
|
G

V

V

R
|
R

D

D

F
x
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

Q

D

G

G

H

H

L

V

A

V

A

A

L

M

A

E

A

K

L

L

A

A

R

N

G

K

E

P

G

G

M

V

L

H

T

I

V

Y

N

N

L

L

G

G

T

A

G

G

R

V

G

G

Y

N

S

S

V
|
V

L

L

D

D

L

V

V

V

R

N

A

A

F

F

E

S

A

K

A

A

S

C

G

G

R

K

T

P

I

V

P

N

F

Y

D

H

F

F

V

A

P

P

R

R

R
|
R

P

E

G

G

D
|
D

I

L

A

P

A

A

C
x
Y

Y

W

A

A

D

D

P

A

A

S

L

K

A

A

A

D

S

R

L

E

L

L

G

N

W

W

T

R

A

V

R

T

R

R

D

T

L

L

A

D

A

E

M

M

C

A

R

Q

D

D

T

T

W

W

R

H

W

W

Q

Q

active site: A124 (≠ S124), A125 (= A125), T126 (= T126), F149 (≠ Y148), K153 (= K152)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), S9 (≠ L9), G10 (= G10), Y11 (= Y11), I12 (= I12), D31 (= D31), N32 (= N32), L33 (= L33), C34 (≠ S34), N35 (= N35), S36 (= S36), G57 (= G57), D58 (= D58), I59 (≠ V59), R60 (= R60), F80 (= F80), A81 (= A81), G82 (= G82), L83 (= L83), K84 (= K84), N99 (≠ C99), S122 (= S122), S123 (= S123), A124 (≠ S124), F149 (≠ Y148), K153 (= K152), Y177 (= Y176), P180 (= P179)
binding uridine-5'-diphosphate-glucose: A124 (≠ S124), A125 (= A125), T126 (= T126), F149 (≠ Y148), F178 (= F177), N179 (= N178), N198 (= N197), N199 (= N198), L200 (= L199), Y203 (= Y202), L215 (= L214), A216 (≠ S215), I217 (≠ V216), F218 (= F217), G229 (= G228), R231 (= R230), Y233 (≠ F232), V269 (= V268), R292 (= R291), D295 (= D294), Y299 (≠ C298)

1hzjA Human udp-galactose 4-epimerase: accommodation of udp-n- acetylglucosamine within the active site (see paper)
56% identity, 97% coverage: 3:329/337 of query aligns to 4:337/345 of 1hzjA

query
sites
1hzjA

I

V

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

Y
|
Y

I
|
I

G

G

S

S

H

H

T

T

C

V

V

L

E

E

L

L

Q

E

A

A

G

G

H

Y

G

L

V

P

T

V

I

V

L

I

D
|
D

N
|
N

L
x
F

S
x
H

N
|
N

-
x
A

-

F

-

R

-

G

S

S

R

L

A

P

D

E

V

S

A

L

E

R

R

R

I

V

A

Q

G

E

L

L

G

T

G

G

A

R

A

S

P

V

I

E

F

F

I

E

E

E

G
x
M

D
|
D

V
x
I

R

L

D

D

R

Q

Q

G

L

A

L

L

C

Q

R

R

I

L

F

F

R

K

E

K

Q

Y

T

S

V

F

D

M

A

A

V

V

I

I

H

H

F
|
F

A
|
A

G
|
G

L

L

K
|
K

A

A

V
|
V

G

G

E

E

S

S

V

V

A

Q

Q

K

P

P

L

L

R

D

Y

Y

E

R

C
x
V

N

N

V

L

G

T

G

G

S

T

L

I

V

Q

L

L

F

L

E

E

A

I

M

M

A

K

E

A

A

H

G

G

V

V

K

K

T

N

L

L

V

V

F

F

S
|
S

A
|
A

T
|
T

V

V

Y

Y

G

G

D

N

P

P

H

Q

A

Y

V

L

P

P

I

L

R

D

E

E

D

A

F

H

P

P

L

T

R

G

-

G

A

C

T

T

N

N

P

P

Y
|
Y

G

G

R

K

S

S

K
|
K

L

F

M

F

L

I

E

E

V

M

L

I

A

R

D

D

M

L

A

C

A

Q

A

A

D

D

S

K

Q

T

W

W

R

N

I

V

A

V

R

L

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

T

G

G

A

A

H

H

E

A

S

S

G

G

L

C

L

I

G

G

E

E

A

D

P

P

N

Q

G

G

I

I

P

P

N
|
N

L
|
L

M

M

P

P

Y

Y

V

V

A

S

Q

Q

V

V

A

A

A

I

G

G

L

R

R

R

A

E

R

A

L
|
L

S
x
N

V
|
V

F
|
F

G

G

N

N

D

D

Y

Y

P

D

T

T

P

E

D

D

G

G

T

T

G
|
G

V

V

R
|
R

D

D

F
x
Y

I

I

H

H

V

V

D

D

L

L

A

A

Q

K

G

G

H

H

L

I

A

A

L

L

A

R

A

K

L

L

A

K

R

E

G

Q

E

C

G

G

M

C

L

R

T

I

V

Y

N

N

L

L

G

G

T

T

G

G

R

T

G

G

Y

Y

S

S

V
|
V

L

L

D

Q

L

M

V

V

R

Q

A

A

F

M

E

E

A

K

A

A

S

S

G

G

R

K

T

K

I

I

P

P

F

Y

D

K

F

V

V

V

P

A

R

R

R
|
R

P

E

G

G

D
|
D

I

V

A

A

C

C

Y

Y

A

A

D

N

P

P

A

S

L

L

A

A

A

Q

S

E

L

E

L

L

G

G

W

W

T

T

A

A

R

A

R

L

D

G

L

L

A

D

A

R

M

M

C

C

R

E

D

D

T

L

W

W

R

R

W

W

Q

Q

Q

K

active site: S131 (= S124), A132 (= A125), T133 (= T126), Y156 (= Y148), K160 (= K152)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (= Y11), I13 (= I12), D32 (= D31), N33 (= N32), F34 (≠ L33), H35