SitesBLAST
Comparing Dsui_0501 FitnessBrowser__PS:Dsui_0501 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 17 hits to proteins with known functional sites (download)
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
25% identity, 96% coverage: 3:403/416 of query aligns to 4:348/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (≠ L6), G8 (= G7), G10 (= G9), V11 (= V10), I12 (≠ V11), V30 (= V29), E31 (≠ D30), K32 (≠ R31), E38 (= E38), A39 (≠ T39), S40 (= S40), A43 (≠ N43), G45 (= G45), L46 (≠ Q46), V171 (= V224), G200 (≠ L253), G201 (= G254), W203 (≠ Y256), G298 (= G354), R300 (= R356), P301 (= P357), Y326 (≠ G381), R327 (≠ T382), N328 (≠ L383), G329 (= G384), I330 (≠ W385)
6j39A Crystal structure of cmis2 with inhibitor (see paper)
27% identity, 72% coverage: 107:405/416 of query aligns to 57:358/368 of 6j39A
- binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: R243 (≠ V291), Y252 (≠ I300), Y267 (≠ A315), R308 (= R356), R334 (≠ T382), I335 (≠ L383)
- binding flavin-adenine dinucleotide: A174 (≠ P223), A203 (≠ L253), W206 (≠ Y256), I228 (≠ A281), Y252 (≠ I300), R308 (= R356), S333 (≠ G381), R334 (≠ T382), I335 (≠ L383), G336 (= G384), V337 (≠ W385), Q338 (≠ T386)
Sites not aligning to the query:
- binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: 44, 46, 49
- binding flavin-adenine dinucleotide: 7, 9, 10, 11, 29, 30, 31, 32, 36, 37, 38, 40, 41, 42, 43, 44
6j38A Crystal structure of cmis2 (see paper)
27% identity, 72% coverage: 107:405/416 of query aligns to 57:358/368 of 6j38A
- binding flavin-adenine dinucleotide: A174 (≠ P223), A203 (≠ L253), W206 (≠ Y256), G226 (= G275), G306 (= G354), R308 (= R356), S333 (≠ G381), R334 (≠ T382), I335 (≠ L383), G336 (= G384), V337 (≠ W385), Q338 (≠ T386)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 7, 9, 11, 29, 30, 31, 36, 37, 38, 41, 42, 43, 44
Q50LF2 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain U-96) (see 4 papers)
24% identity, 60% coverage: 161:410/416 of query aligns to 131:384/405 of Q50LF2
- K172 (≠ D199) mutation to A: Retains 39% of wild-type activity.; mutation to D: Retains 32% of wild-type activity.; mutation to R: Retains 58% of wild-type activity.
- H173 (≠ A200) modified: Tele-8alpha-FMN histidine
- V197 (= V224) binding
- H270 (≠ R298) mutation to A: 10-fold decrease in catalytic efficiency.
- Y272 (≠ I300) mutation to A: 13000-fold decrease in catalytic efficiency.; mutation to F: 130-fold decrease in catalytic efficiency.
- G354 (= G381) binding
- G357 (= G384) binding
- K359 (≠ T386) binding ; mutation K->A,D: Loss of activity.; mutation to R: Retains 0.07% of wild-type activity. Shows higher apparent KM for sarcosine.
Sites not aligning to the query:
- 31 binding
- 32 binding
- 53 binding
- 61 binding
- 62 binding
- 66 binding
- 68 binding
2gagB Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
22% identity, 71% coverage: 115:410/416 of query aligns to 83:382/403 of 2gagB
- binding flavin-adenine dinucleotide: V195 (= V224), G224 (≠ L253), A225 (≠ G254), H227 (≠ Y256), L231 (= L260), L246 (≠ Y276), G352 (= G381), T353 (= T382), G354 (≠ L383), G355 (= G384), F356 (≠ W385), K357 (≠ T386)
- binding flavin mononucleotide: H171 (≠ A200), V250 (≠ I280), E278 (≠ N307), R321 (≠ L350), W323 (= W352)
- binding 2-furoic acid: M263 (≠ I293), Y270 (≠ I300), K357 (≠ T386)
- binding sulfite ion: K170 (≠ D199), K276 (≠ L305)
Sites not aligning to the query:
- active site: 61, 64, 65
- binding flavin-adenine dinucleotide: 26, 28, 29, 30, 51, 52, 58, 59, 60, 62, 63, 64, 66
- binding flavin mononucleotide: 61, 62
- binding 2-furoic acid: 64, 66, 68, 401
3ad8B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with pyrrole 2-carboxylate (see paper)
24% identity, 60% coverage: 161:410/416 of query aligns to 130:383/404 of 3ad8B
- active site: G326 (= G354), K358 (≠ T386)
- binding flavin-adenine dinucleotide: V196 (= V224), G225 (≠ L253), A226 (≠ G254), H228 (≠ Y256), L247 (≠ Y276), G353 (= G381), T354 (= T382), G355 (≠ L383), G356 (= G384), F357 (≠ W385), K358 (≠ T386)
- binding flavin mononucleotide: H172 (≠ A200), V251 (≠ I280), E279 (≠ N307), R322 (≠ L350), W324 (= W352)
- binding pyrrole-2-carboxylate: M264 (≠ I293), Y271 (≠ I300), T354 (= T382), K358 (≠ T386)
Sites not aligning to the query:
- active site: 62, 65, 66
- binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
- binding flavin mononucleotide: 62, 63
- binding pyrrole-2-carboxylate: 65, 67, 69, 402
3ad7B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with methylthio acetate (see paper)
24% identity, 60% coverage: 161:410/416 of query aligns to 130:383/404 of 3ad7B
- active site: G326 (= G354), K358 (≠ T386)
- binding flavin-adenine dinucleotide: V196 (= V224), G225 (≠ L253), A226 (≠ G254), H228 (≠ Y256), L247 (≠ Y276), G353 (= G381), T354 (= T382), G355 (≠ L383), G356 (= G384), F357 (≠ W385), K358 (≠ T386)
- binding flavin mononucleotide: H172 (≠ A200), V251 (≠ I280), K277 (≠ L305), E279 (≠ N307), R322 (≠ L350), W324 (= W352)
- binding [methylthio]acetate: M264 (≠ I293), Y271 (≠ I300), T354 (= T382), K358 (≠ T386)
Sites not aligning to the query:
- active site: 62, 65, 66
- binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
- binding flavin mononucleotide: 62, 63
- binding [methylthio]acetate: 67, 69, 402
1vrqB Crystal structure of heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with folinic acid (see paper)
24% identity, 60% coverage: 161:410/416 of query aligns to 130:383/402 of 1vrqB
- active site: G326 (= G354), K358 (≠ T386)
- binding n,n-dimethylglycine: K358 (≠ T386)
- binding flavin-adenine dinucleotide: V196 (= V224), A224 (= A252), G225 (≠ L253), H228 (≠ Y256), L247 (≠ Y276), G353 (= G381), T354 (= T382), G355 (≠ L383), G356 (= G384), F357 (≠ W385), K358 (≠ T386)
- binding flavin mononucleotide: H172 (≠ A200), V251 (≠ I280), E279 (≠ N307), R322 (≠ L350), W324 (= W352)
Sites not aligning to the query:
- active site: 62, 65, 66
- binding n,n-dimethylglycine: 65, 67, 69, 401
- binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 51, 52, 53, 59, 60, 61, 63, 64, 65, 67
- binding flavin mononucleotide: 62, 63
S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
30% identity, 44% coverage: 218:401/416 of query aligns to 169:348/369 of S5FMM4
- S202 (≠ L253) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
- I332 (≠ W385) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
- M342 (≠ L395) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.
Sites not aligning to the query:
- 51 G→S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
- 54 A→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
- 81 K→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
Q5L2C2 Glycine oxidase; GO; GOX; GOXK; EC 1.4.3.19 from Geobacillus kaustophilus (strain HTA426)
25% identity, 73% coverage: 108:410/416 of query aligns to 62:365/377 of Q5L2C2
- V180 (≠ P223) binding
- R309 (= R356) binding
- 334:340 (vs. 380:386, 29% identical) binding
- R336 (≠ T382) binding
Sites not aligning to the query:
- 14:15 binding
- 34:35 binding
- 42:43 binding
- 47:49 binding
4yshA Crystal structure of glycine oxidase from geobacillus kaustophilus
24% identity, 73% coverage: 108:410/416 of query aligns to 61:363/370 of 4yshA
- active site: I262 (≠ V311), L283 (= L332), G305 (= G354), N335 (≠ L383), L338 (≠ T386)
- binding flavin-adenine dinucleotide: V178 (≠ P223), S206 (≠ L253), G207 (= G254), W209 (≠ Y256), R307 (= R356), H332 (= H380), R334 (≠ T382), N335 (≠ L383), G336 (= G384), I337 (≠ W385)
Sites not aligning to the query:
- active site: 45, 48, 49, 52
- binding flavin-adenine dinucleotide: 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 47, 48
O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
24% identity, 54% coverage: 184:408/416 of query aligns to 134:355/369 of O31616
- V174 (= V224) binding
- H244 (≠ E297) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
- R302 (= R356) binding
- 327:333 (vs. 380:386, 29% identical) binding
- R329 (≠ T382) binding
Sites not aligning to the query:
- 14:15 binding
- 34:35 binding
- 42:43 binding
- 47:49 binding
- 51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
- 54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
P40875 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain P-1) (see 3 papers)
24% identity, 67% coverage: 122:400/416 of query aligns to 88:373/405 of P40875
- C146 (≠ E176) mutation to S: No change in activity.
- H173 (≠ A200) modified: Tele-8alpha-FMN histidine; mutation to N: Prevents covalent attachment of FMN. Loss of activity. The mutant is considerably less stable than wild-type enzyme, the beta and delta subunits are lost during purification, which yields a stable alpha-gamma complex.
- H175 (≠ R202) mutation to A: No effect on FMN binding and activity.
- C195 (= C222) mutation to S: No change in activity.
- C351 (≠ T378) mutation to A: No change in activity.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 30 G→A: Prevents covalent attachment of FMN. Blocks subunit assembly.
4yshB Crystal structure of glycine oxidase from geobacillus kaustophilus
24% identity, 73% coverage: 108:410/416 of query aligns to 61:363/368 of 4yshB
- active site: I262 (≠ V311), L283 (= L332), G305 (= G354), N335 (≠ L383), L338 (≠ T386)
- binding flavin-adenine dinucleotide: V178 (≠ P223), S206 (≠ L253), W209 (≠ Y256), R307 (= R356), H332 (= H380), R334 (≠ T382), N335 (≠ L383), G336 (= G384), I337 (≠ W385), L338 (≠ T386)
- binding glycine: G249 (≠ E297), Y251 (≠ K299), Y251 (≠ K299), A264 (≠ G313), R307 (= R356), R334 (≠ T382), R334 (≠ T382)
Sites not aligning to the query:
- active site: 45, 48, 49, 52
- binding flavin-adenine dinucleotide: 9, 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 48
1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
24% identity, 54% coverage: 184:408/416 of query aligns to 134:355/364 of 1ng3A
- binding acetylamino-acetic acid: Y246 (≠ K299), R302 (= R356), R329 (≠ T382)
- binding flavin-adenine dinucleotide: V174 (= V224), S202 (≠ L253), G203 (= G254), W205 (≠ Y256), F209 (≠ L260), G300 (= G354), R302 (= R356), H327 (= H380), R329 (≠ T382), N330 (≠ L383), G331 (= G384), I332 (≠ W385)
- binding phosphate ion: R254 (= R308)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49
- binding phosphate ion: 89
Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
26% identity, 72% coverage: 103:401/416 of query aligns to 76:386/824 of Q8GAI3
Sites not aligning to the query:
- 66 mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
- 67 H→A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.
3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
24% identity, 54% coverage: 184:408/416 of query aligns to 134:355/364 of 3if9A
- binding flavin-adenine dinucleotide: P173 (= P223), V174 (= V224), S202 (≠ L253), G203 (= G254), W205 (≠ Y256), F209 (≠ L260), G300 (= G354), R302 (= R356), H327 (= H380), F328 (≠ G381), R329 (≠ T382), N330 (≠ L383), G331 (= G384), I332 (≠ W385)
- binding glycolic acid: Y246 (≠ K299), R302 (= R356), R329 (≠ T382)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 34, 35, 42, 43, 46, 47, 48, 49
Query Sequence
>Dsui_0501 FitnessBrowser__PS:Dsui_0501
MRVLVLGAGVVGVTSAWFLAEAGHEVTVVDRQPGAALETSFANGGQISVCHAEPWANPRA
PFKALEWLGKEDAPLLFRLRYDPALFAWSLRFLANCPPGATRRNIRDIIALALYSRQRLQ
ALRQALPLDYDQRCQGILHIFTQAAEFEAACHAAALMREFGVDREPVDAARCVAIEPALA
AVQGRLAGGDYTPSDESGDAHRFTQRLAEAAAARGVQFRYNCPVEKIASAGGRVAGVVAG
GDLLLADAYVVALGSYSPALLKPAGVKACVYPGKGYSATIALSPDSVAPSVSITDDERKI
VMSRLGNRLRVAGTAEFNGHNLDLTPVRCEALLRRALELFPQLRPDGDPLYWCGLRPVTP
SNVPLIGRTRLSNLWLNTGHGTLGWTLSCGSAAALADLISGRRPEPDFPFLGTTKQ
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory