SitesBLAST
Comparing Dsui_0543 FitnessBrowser__PS:Dsui_0543 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P76558 NADP-dependent malic enzyme; NADP-ME; EC 1.1.1.40 from Escherichia coli (strain K12) (see paper)
64% identity, 100% coverage: 1:757/758 of query aligns to 1:757/759 of P76558
- K56 (≠ A56) modified: N6-acetyllysine
6zngF Maeb full-length acetyl-coa bound state (see paper)
49% identity, 99% coverage: 4:755/758 of query aligns to 3:744/753 of 6zngF
- active site: Y38 (= Y39), A74 (= A75), K93 (= K94), E135 (= E136), D136 (= D137), D160 (= D161), D161 (= D162), N286 (= N287)
- binding acetyl coenzyme *a: R511 (≠ M518), K514 (≠ R521), Y552 (≠ F560), A553 (= A561), R557 (= R565), L560 (≠ E568), P571 (≠ L580), T590 (≠ H599), V591 (= V600), N592 (= N601), L593 (≠ E602), Y625 (≠ H634), Q659 (≠ H669), L690 (= L700), N694 (= N704), Q724 (≠ T735)
6zn7A Maeb malic enzyme domain apoprotein (see paper)
60% identity, 53% coverage: 4:407/758 of query aligns to 2:405/405 of 6zn7A
- active site: Y37 (= Y39), A73 (= A75), K92 (= K94), E134 (= E136), D135 (= D137), D159 (= D161), D160 (= D162), N285 (= N287)
- binding magnesium ion: E134 (= E136), D135 (= D137), D160 (= D162)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: T164 (= T166), N191 (≠ S193), A193 (= A195), G194 (= G196), A195 (= A197), S196 (≠ A198), D218 (= D220), S219 (= S221), K235 (= K237), L260 (= L262), S261 (= S263), V262 (≠ A264), M283 (≠ L285), N285 (= N287), V315 (= V317)
6zn4A Maeb malic enzyme domain apoprotein (see paper)
60% identity, 53% coverage: 4:407/758 of query aligns to 2:405/406 of 6zn4A
2dvmA NAD complex structure of ph1275 protein from pyrococcus horikoshii
45% identity, 53% coverage: 5:405/758 of query aligns to 1:397/438 of 2dvmA
- active site: Y37 (= Y39), R73 (≠ A75), K92 (= K94), E134 (= E136), D135 (= D137), D159 (= D161), D160 (= D162), N296 (= N287)
- binding nicotinamide-adenine-dinucleotide: T164 (= T166), G194 (= G196), A195 (= A197), A196 (= A198), V217 (≠ C219), E218 (≠ D220), L219 (vs. gap), P224 (≠ K222), F269 (≠ L262), T270 (≠ S263), L294 (= L285), N296 (= N287), N327 (= N319)
5ceeA Malic enzyme from candidatus phytoplasma aywb in complex with NAD and mg2+ (see paper)
46% identity, 53% coverage: 5:404/758 of query aligns to 1:385/387 of 5ceeA
- active site: Y34 (= Y39), A70 (= A75), K89 (= K94), E131 (= E136), D132 (= D137), D156 (= D161), D157 (= D162), N283 (= N287)
- binding magnesium ion: E131 (= E136), D132 (= D137), D157 (= D162)
- binding nicotinamide-adenine-dinucleotide: T161 (= T166), N188 (≠ S193), G189 (= G194), G191 (= G196), A193 (= A198), D213 (= D220), K214 (≠ S221), V258 (≠ L262), S259 (= S263), I263 (≠ V267), L281 (= L285), N283 (= N287), V312 (= V317), N314 (= N319)
2a9fA Crystal structure of a putative malic enzyme ((s)-malate:nad+ oxidoreductase (decarboxylating))
47% identity, 47% coverage: 1:357/758 of query aligns to 1:354/383 of 2a9fA
2haeD Crystal structure of a putative malic enzyme (malate oxidoreductase)
46% identity, 50% coverage: 9:384/758 of query aligns to 2:358/373 of 2haeD
2haeB Crystal structure of a putative malic enzyme (malate oxidoreductase)
46% identity, 50% coverage: 9:384/758 of query aligns to 2:358/373 of 2haeB
- active site: Y31 (= Y39), A67 (= A75), K86 (= K94), E128 (= E136), D129 (= D137), D153 (= D161), D154 (= D162), N280 (= N287)
- binding nicotinamide-adenine-dinucleotide: T158 (= T166), N185 (≠ S193), G188 (= G196), A189 (= A197), A190 (= A198), D210 (= D220), R211 (≠ S221), V255 (≠ L262), S256 (= S263), R257 (≠ A264), L278 (= L285), A279 (= A286), N280 (= N287), N311 (= N319)
Q8ZND6 Phosphate acetyltransferase; Phosphotransacetylase; EC 2.3.1.222; EC 2.3.1.8 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
32% identity, 42% coverage: 444:758/758 of query aligns to 405:713/714 of Q8ZND6
Sites not aligning to the query:
- 252 R→H: Increases speed of forward and back reactions by 2-3 fold. Not inhibited by NADH.
- 273 G→D: Increases speed of forward and back reactions by 2-3 fold.
- 294 M→I: Slightly decreases speed of forward and back reactions.
1xcoD Crystal structure of a phosphotransacetylase from bacillus subtilis in complex with acetylphosphate (see paper)
29% identity, 43% coverage: 429:754/758 of query aligns to 2:324/325 of 1xcoD
6ioxA Crystal structure of porphyromonas gingivalis phosphotransacetylase in complex with acetyl-coa (see paper)
33% identity, 42% coverage: 439:756/758 of query aligns to 16:339/339 of 6ioxA
2af3C Phosphotransacetylase from methanosarcina thermophila soaked with coenzyme a (see paper)
30% identity, 41% coverage: 449:757/758 of query aligns to 22:330/332 of 2af3C
- binding coenzyme a: R86 (≠ M518), S127 (≠ F560), L131 (= L564), V135 (≠ E568), L146 (≠ T579), A147 (≠ L580), G172 (≠ H599), M173 (≠ V600), M173 (≠ V600), V174 (≠ N601), E175 (= E602), N278 (= N704), Y281 (= Y707), K282 (≠ N708), Q285 (≠ K711), G294 (= G721), P295 (= P722), T297 (≠ L724), D306 (≠ V733), L307 (= L734), S308 (≠ T735)
P38503 Phosphate acetyltransferase; Phosphotransacetylase; EC 2.3.1.8 from Methanosarcina thermophila (see 2 papers)
30% identity, 41% coverage: 449:757/758 of query aligns to 23:331/333 of P38503
- C159 (vs. gap) mutation to A: Loss of activity.; mutation to S: No loss of activity.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
P16243 NADP-dependent malic enzyme, chloroplastic; NADP-ME; EC 1.1.1.40 from Zea mays (Maize) (see 3 papers)
27% identity, 50% coverage: 35:415/758 of query aligns to 180:622/636 of P16243
- R237 (≠ A75) mutation to L: Decreases kcat 530-fold. Increases Km for NADP 36-fold and Km for malate 10-fold.
- K255 (= K94) mutation to I: Increases Km for malate 10-fold, and Km for NADP 15-fold. Decreases kcat 200-fold.
- E339 (= E148) mutation to A: No effect on kcat and Km for NADP. Increases Km for malate 2-fold.
- A387 (= A198) mutation to G: Decreases kcat 48-fold. Increases Km for NADP 4-fold. Increases Km for malate 6-fold.
- A392 (≠ L203) mutation to G: No effect on kcat. Increases Km for NADP 3.5-fold. Increases Km for malate 2.5-fold.
- KK 435:436 (≠ KA 237:238) mutation to LL: No effect on kcat and on Km for malate. Increases Km for NADP 9-fold.
- Q503 (≠ L296) mutation to E: No effect on kcat and Km for NADP. Increases Km for malate 2-fold.
- L544 (≠ I326) mutation to F: No effect on kcat and Km for NADP. Increases Km for malate 2-fold.
Sites not aligning to the query:
- 140 F→I: Decreases kcat 8-fold. Decreases Km for NADP 2-fold, increases Km for malate 2-fold.
1gq2A Malic enzyme from pigeon liver (see paper)
26% identity, 44% coverage: 32:361/758 of query aligns to 83:480/555 of 1gq2A
- active site: Y90 (= Y39), R143 (≠ A75), K161 (= K94), E233 (= E136), D234 (= D137), D256 (= D161), D257 (= D162), N396 (= N287)
- binding manganese (ii) ion: K161 (= K94), E233 (= E136), D234 (= D137), D257 (= D162)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R143 (≠ A75), G146 (= G78), N237 (≠ A140), T261 (= T166), G289 (= G194), A290 (= A195), G291 (= G196), E292 (≠ A197), A293 (= A198), V322 (≠ C219), D323 (= D220), S324 (= S221), A368 (≠ S263), I370 (vs. gap), L394 (= L285), N396 (= N287), G440 (≠ V317), N441 (= N318), N442 (= N319)
- binding oxalate ion: R143 (≠ A75), L145 (= L77), D257 (= D162), N396 (= N287), N442 (= N319)
P40927 NADP-dependent malic enzyme; NADP-ME; EC 1.1.1.40 from Columba livia (Rock dove) (see 3 papers)
26% identity, 44% coverage: 32:361/758 of query aligns to 84:481/557 of P40927
- D141 (≠ N72) mutation to N: Increases Km for manganese 14-fold. Increases Km for malate 5-fold.
- R144 (≠ A75) binding ; binding
- K162 (= K94) binding ; mutation to A: Decreases kcat 235-fold. no effect on Km for NADP.
- D194 (= D115) mutation to N: No effect on Km for manganese. Increases Km for malate 8-fold.
- E234 (= E136) binding
- D235 (= D137) binding
- N238 (≠ A140) binding
- D258 (= D162) binding
- AGEA 291:294 (≠ AGAA 195:198) binding
- S325 (= S221) binding
- K340 (= K237) mutation to A: Increases Km for NADP 65-fold. No effect on kcat.
- N397 (= N287) binding
- N443 (= N319) binding ; binding
- D464 (≠ E340) mutation to N: No effect.
6w49A Trypanosoma cruzi malic enzyme in complex with inhibitor (mec010) (see paper)
27% identity, 38% coverage: 67:356/758 of query aligns to 131:481/543 of 6w49A
Sites not aligning to the query:
6w29A Trypanosoma cruzi malic enzyme in complex with inhibitor (mec013) (see paper)
27% identity, 38% coverage: 67:356/758 of query aligns to 133:483/545 of 6w29A
Sites not aligning to the query:
6w59A Trypanosoma cruzi malic enzyme in complex with inhibitor (mec063) (see paper)
27% identity, 38% coverage: 67:356/758 of query aligns to 131:481/544 of 6w59A
Sites not aligning to the query:
Query Sequence
>Dsui_0543 FitnessBrowser__PS:Dsui_0543
MEKDLREAALEYHRLPTPGKIAVRPTKGLTNQRDLALAYSPGVAYACEEIVANPNAVDEM
TSRGNLVGVVTNGTAVLGLGNIGPLAAKPVMEGKGCLFKKFAGIDVFDIEISENDPDKLI
DMIAALEPTLGGVNLEDIKAPECFYIEEKLKERMSIPVFHDDQHGTAIISAAAMLNGLKV
IGKDIASVKVVCSGAGAAAISCLNLWCELGIKRENVTVCDSKGVIYIGRDAKMEATKARY
AQETSARTLADAIVGADIFLGLSAAGVLKAEMVKQMAKDPLIFALANPTPEIMPELAKEV
RPDAIIATGRSDYANQVNNVLCFPFIFRGALDVGAKRITEEMKLACVKAIAALAQEEVSD
EVAMAYQGQQLTFGPEYLIPKPFDPRLISIIAPAVARAAMESGVATRPIVDMDAYRDKLK
EFNTHTGVGMRAVFSAAKQGEASRVIYAEGEDERVLRAIQVILEERLAKPILVGRPEVIE
ARLQKIGSKLKAGEHFEVVNPNYDERYKECWSAYHQLMKRRGVTIDIAKMRLRSDNTIIG
AMLVKLGYADALICGMSGRFAHHLRQVEEVIGKAAGHNTLAAMNHLLLPNRALFLCDTHV
NENPDAQQLADIAVMAAETVRSFGITPKVALLSHSNFGSSQSASARKMSEAADIARAKAP
DLEIDGEMHGDSALSEEIRLQSDPESPLKGEANVLVMPNLDAANISYNLLKMTGGEGVTI
GPILMGVAKPVHVLTATATVRRLVNMTALAVVDAKKQR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory