SitesBLAST
Comparing Dsui_0575 FitnessBrowser__PS:Dsui_0575 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 11 hits to proteins with known functional sites (download)
6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
48% identity, 91% coverage: 22:347/360 of query aligns to 65:386/393 of 6fahE
- binding flavin-adenine dinucleotide: L180 (= L140), R200 (= R160), M281 (≠ R242), G282 (= G243), R307 (= R268), A308 (≠ P269), Q320 (= Q281), V321 (= V282), G322 (= G283), Q323 (= Q284), T324 (≠ S285), G337 (= G298), I338 (= I299), S339 (= S300), Q343 (= Q304), H344 (= H305), N358 (= N319), K359 (≠ T320), L377 (≠ A338)
Sites not aligning to the query:
- binding iron/sulfur cluster: 7, 10, 13, 17, 18, 35, 36, 37, 38, 41, 45, 49
7koeB Electron bifurcating flavoprotein fix/etfabcx (see paper)
47% identity, 93% coverage: 23:356/360 of query aligns to 5:334/336 of 7koeB
- binding flavin-adenine dinucleotide: T121 (≠ V141), R140 (= R160), T142 (= T162), G219 (= G241), K220 (≠ R242), G221 (= G243), S245 (= S267), R246 (= R268), A247 (≠ P269), Q259 (= Q281), V260 (= V282), G261 (= G283), Q262 (= Q284), T263 (≠ S285), G276 (= G298), S278 (= S300), Q282 (= Q304), H283 (= H305), N297 (= N319), I298 (≠ T320), L316 (≠ A338)
4kpuA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
43% identity, 92% coverage: 21:351/360 of query aligns to 9:334/338 of 4kpuA
- binding flavin-adenine dinucleotide: L125 (= L140), R144 (= R160), I155 (= I171), G224 (= G241), R225 (= R242), G226 (= G243), S250 (= S267), R251 (= R268), A252 (≠ P269), Q264 (= Q281), V265 (= V282), G266 (= G283), Q267 (= Q284), S268 (= S285), G281 (= G298), I282 (= I299), S283 (= S300), S285 (≠ A302), Q287 (= Q304), H288 (= H305), N302 (= N319), K303 (≠ T320), D320 (≠ N337), A321 (= A338)
5ol2A The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
40% identity, 94% coverage: 24:360/360 of query aligns to 3:331/331 of 5ol2A
- binding calcium ion: E75 (= E99), D188 (≠ S212)
- binding flavin-adenine dinucleotide: T117 (≠ V141), R136 (= R160), I147 (= I171), G216 (= G241), R217 (= R242), G218 (= G243), S242 (= S267), R243 (= R268), A244 (≠ P269), Q256 (= Q281), V257 (= V282), G258 (= G283), T260 (≠ S285), G273 (= G298), I274 (= I299), S275 (= S300), A277 (= A302), Q279 (= Q304), H280 (= H305), N294 (= N319), K295 (≠ T320), D312 (≠ N337), V313 (≠ A338)
7qh2A Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
37% identity, 93% coverage: 11:345/360 of query aligns to 1:327/337 of 7qh2A
- binding flavin-adenine dinucleotide: L125 (= L140), T126 (≠ V141), R144 (= R160), I155 (= I171), R224 (= R242), G225 (= G243), T249 (≠ S267), R250 (= R268), Q263 (= Q281), I264 (≠ V282), G265 (= G283), L266 (≠ Q284), S267 (= S285), G280 (= G298), I281 (= I299), S282 (= S300), Q286 (= Q304), N301 (= N319), S302 (≠ T320), D303 (= D321), D319 (≠ N337), L320 (≠ A338)
P13804 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Homo sapiens (Human) (see 6 papers)
33% identity, 92% coverage: 19:350/360 of query aligns to 16:331/333 of P13804
- 20:204 (vs. 23:214, 20% identical) Domain I
- G116 (= G125) to R: in GA2A; impaired protein stability and loss of electron transfer activity; dbSNP:rs119458971
- T171 (≠ P185) to I: decreased protein stability; dbSNP:rs1801591
- R223 (= R242) binding FAD
- S248 (= S267) binding FAD
- R249 (= R268) mutation to A: Loss of electron transfer activity.
- VGQT 263:266 (≠ VGQS 282:285) binding FAD
- T266 (≠ S285) to M: in GA2A; decreased electron transfer activity; dbSNP:rs119458970
- SGAIQH 281:286 (= SGAIQH 300:305) binding FAD
- N300 (= N319) binding FAD
- DL 318:319 (≠ NA 337:338) binding FAD
Sites not aligning to the query:
2a1uA Crystal structure of the human etf e165betaa mutant (see paper)
33% identity, 91% coverage: 22:350/360 of query aligns to 1:313/315 of 2a1uA
- binding flavin-adenine dinucleotide: G204 (= G241), R205 (= R242), S230 (= S267), R231 (= R268), A232 (≠ P269), Q244 (= Q281), V245 (= V282), G246 (= G283), T248 (≠ S285), G261 (= G298), I262 (= I299), S263 (= S300), A265 (= A302), Q267 (= Q304), H268 (= H305), N282 (= N319), K283 (≠ T320), D300 (≠ N337), L301 (≠ A338)
1efpA Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
34% identity, 73% coverage: 84:346/360 of query aligns to 55:304/307 of 1efpA
- binding flavin-adenine dinucleotide: G199 (= G241), R200 (= R242), G201 (= G243), S225 (= S267), R226 (= R268), A227 (≠ P269), Q239 (= Q281), V240 (= V282), G241 (= G283), T243 (≠ S285), G256 (= G298), I257 (= I299), S258 (= S300), A260 (= A302), Q262 (= Q304), H263 (= H305), N277 (= N319), K278 (≠ T320), D295 (≠ N337), L296 (≠ A338)
P53571 Electron transfer flavoprotein subunit alpha; Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS from Methylophilus methylotrophus (Bacterium W3A1) (see 2 papers)
31% identity, 92% coverage: 25:354/360 of query aligns to 4:320/321 of P53571
- R211 (= R242) binding FAD
- SR 236:237 (= SR 267:268) binding FAD
- QVGQS 250:254 (= QVGQS 281:285) binding FAD
- 268:275 (vs. 298:305, 88% identical) binding FAD
- N289 (= N319) binding FAD
- DI 307:308 (≠ NA 337:338) binding FAD
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3clrD Crystal structure of the r236a etf mutant from m. Methylotrophus (see paper)
30% identity, 92% coverage: 25:354/360 of query aligns to 3:319/319 of 3clrD
- binding flavin-adenine dinucleotide: G209 (= G241), R210 (= R242), G211 (= G243), S235 (= S267), A236 (≠ R268), P237 (= P269), Q249 (= Q281), V250 (= V282), G251 (= G283), Q252 (= Q284), S253 (= S285), G267 (= G298), I268 (= I299), S269 (= S300), S271 (≠ A302), Q273 (= Q304), H274 (= H305), N288 (= N319), T289 (= T320), D306 (≠ N337), I307 (≠ A338)
5ow0A Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
28% identity, 88% coverage: 36:350/360 of query aligns to 6:292/292 of 5ow0A
- binding flavin-adenine dinucleotide: G183 (= G241), R184 (= R242), G185 (= G243), S209 (= S267), R210 (= R268), Q223 (= Q281), I224 (≠ V282), G225 (= G283), T227 (≠ S285), G240 (= G298), V241 (≠ I299), S242 (= S300), A244 (= A302), Q246 (= Q304), H247 (= H305), N261 (= N319), K262 (≠ T320), D279 (≠ N337), Y280 (≠ A338)
Query Sequence
>Dsui_0575 FitnessBrowser__PS:Dsui_0575
MSEQPKKPAKKKVELDPRFVNYKHVWVVVESERGEVHPVSWELLGEGRKLADALGVQLCG
VVMCGPGERGAGICGEAFTYGADKCYLMQDEVLKDYRNEPYTKALTDLVNTYQPEILMLG
ASTLGRDLAGSVATTLGTGLVADCTELVIETETRNLASTRPTFGGSLLCTIMTQRHRPQM
ATVRPRVMAMPEADGSRSGEIVTVPFSMVETSIVTKVLEFIADDTRDKPNLPFADIIVSG
GRGLKKPENFQLVWDLAKVLGAEVGASRPVVQAGWAELDRQVGQSGKTVRPKLYIAAGIS
GAIQHRVGMDGADVIIAINTDANAPIFDFAHYGIVGNAMTILPALTEAFKAHLAQAKKAG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory