SitesBLAST
Comparing Dsui_0576 FitnessBrowser__PS:Dsui_0576 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 14 hits to proteins with known functional sites (download)
7koeA Electron bifurcating flavoprotein fix/etfabcx (see paper)
47% identity, 84% coverage: 4:240/281 of query aligns to 5:241/282 of 7koeA
- binding flavin-adenine dinucleotide: C7 (= C6), I8 (= I7), K9 (= K8), D39 (= D38), M62 (= M61), A93 (≠ S92), D94 (= D93), T95 (= T94), T98 (= T97), L102 (= L101), T121 (= T119), G122 (= G120), A125 (≠ T123), G128 (= G126), T130 (= T128), G131 (≠ A129), V133 (= V131)
6fahB Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
44% identity, 96% coverage: 1:269/281 of query aligns to 1:260/262 of 6fahB
- binding flavin-adenine dinucleotide: C6 (= C6), A7 (≠ I7), K8 (= K8), D38 (= D38), T61 (= T60), M62 (= M61), A93 (≠ S92), D94 (= D93), T95 (= T94), T98 (= T97), L102 (= L101), G118 (= G120), Q120 (= Q122), G124 (= G126), T126 (= T128), A127 (= A129), V129 (= V131), T223 (= T229)
4l2iB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
45% identity, 90% coverage: 1:254/281 of query aligns to 1:244/263 of 4l2iB
- binding flavin-adenine dinucleotide: C6 (= C6), V7 (≠ I7), K8 (= K8), Y37 (= Y37), D38 (= D38), Y40 (≠ F40), T60 (= T60), M61 (= M61), A92 (≠ S92), D93 (= D93), T94 (= T94), T97 (= T97), M101 (≠ L101), C116 (≠ T119), G117 (= G120), Q119 (= Q122), A120 (≠ T123), G123 (= G126), T125 (= T128), A126 (= A129), V128 (= V131)
- binding nicotinamide-adenine-dinucleotide: M61 (= M61), G62 (= G62), P63 (= P63), S85 (= S85), D86 (= D86), G91 (= G91)
4kpuB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
45% identity, 90% coverage: 1:254/281 of query aligns to 1:244/263 of 4kpuB
- binding flavin-adenine dinucleotide: C6 (= C6), V7 (≠ I7), K8 (= K8), Y37 (= Y37), D38 (= D38), Y40 (≠ F40), M61 (= M61), A92 (≠ S92), D93 (= D93), T94 (= T94), T97 (= T97), C116 (≠ T119), G117 (= G120), Q119 (= Q122), A120 (≠ T123), G123 (= G126), T125 (= T128), A126 (= A129), V128 (= V131)
5ol2B The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
46% identity, 84% coverage: 1:235/281 of query aligns to 1:228/260 of 5ol2B
- binding flavin-adenine dinucleotide: C6 (= C6), I7 (= I7), K8 (= K8), D38 (= D38), M61 (= M61), A92 (≠ S92), D93 (= D93), T94 (= T94), T97 (= T97), A115 (≠ T119), G116 (= G120), A119 (≠ T123), G122 (= G126), T124 (= T128), A125 (= A129), V127 (= V131)
7qh2B Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
38% identity, 93% coverage: 4:263/281 of query aligns to 5:256/265 of 7qh2B
- binding flavin-adenine dinucleotide: C7 (= C6), K9 (= K8), D39 (= D38), M62 (= M61), D94 (= D93), V95 (≠ T94), L102 (= L101), G118 (= G120), Q120 (= Q122), T121 (= T123), T126 (= T128), A127 (= A129), V129 (= V131)
Q9DCW4 Electron transfer flavoprotein subunit beta; Beta-ETF from Mus musculus (Mouse) (see paper)
32% identity, 77% coverage: 1:217/281 of query aligns to 4:217/255 of Q9DCW4
- K200 (≠ R200) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (≠ R203) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
Q2TBV3 Electron transfer flavoprotein subunit beta; Beta-ETF from Bos taurus (Bovine) (see paper)
32% identity, 77% coverage: 1:217/281 of query aligns to 4:217/255 of Q2TBV3
- K200 (≠ R200) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (≠ R203) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
P38117 Electron transfer flavoprotein subunit beta; Beta-ETF from Homo sapiens (Human) (see 10 papers)
32% identity, 77% coverage: 1:217/281 of query aligns to 4:217/255 of P38117
- A9 (≠ C6) binding AMP
- NPFC 39:42 (≠ NPYD 35:38) binding AMP
- C66 (≠ M61) binding AMP
- 123:134 (vs. 120:131, 58% identical) binding AMP
- D128 (= D125) to N: in GA2B; decreased protein stability; dbSNP:rs104894678
- T154 (≠ V151) to M: in dbSNP:rs1130426
- R164 (= R164) to Q: in GA2B; reduced electron transfer activity; dbSNP:rs104894677
- E165 (≠ R165) mutation E->A,Q: Drastically increases interprotein electron transfer rates.
- 183:205 (vs. 183:205, 22% identical) Recognition loop
- L195 (= L195) mutation to A: Severely impaired in complex formation with ACADM.
- K200 (≠ R200) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- KAK 200:202 (≠ RAA 200:202) mutation to RAR: Does not abolish methylation by ETFBKMT.
- KAKK 200:203 (≠ RAAR 200:203) mutation to RAKR: Does not abolish electron transfer activity. Abolishes sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- K203 (≠ R203) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
2a1uB Crystal structure of the human etf e165betaa mutant (see paper)
32% identity, 77% coverage: 1:217/281 of query aligns to 1:214/252 of 2a1uB
- binding adenosine monophosphate: A6 (≠ C6), V7 (≠ I7), K8 (= K8), C39 (≠ D38), C63 (≠ M61), V101 (≠ L101), L119 (≠ T119), G120 (= G120), Q122 (= Q122), A123 (≠ T123), D126 (≠ G126), C128 (≠ T128), N129 (≠ A129), Q130 (= Q130), T131 (≠ V131)
- binding flavin-adenine dinucleotide: P37 (= P36), L182 (≠ E185)
1efpB Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
30% identity, 73% coverage: 1:206/281 of query aligns to 1:203/246 of 1efpB
- binding adenosine monophosphate: P6 (≠ C6), V7 (≠ I7), D39 (= D38), I63 (≠ M61), V101 (≠ T97), A119 (≠ T119), G120 (= G120), Q122 (= Q122), A123 (≠ T123), N126 (≠ G126), M128 (≠ T128), N129 (≠ A129), T131 (≠ V131)
- binding flavin-adenine dinucleotide: Y13 (vs. gap), L182 (≠ E185)
1o96A Structure of electron transferring flavoprotein for methylophilus methylotrophus. (see paper)
30% identity, 77% coverage: 34:248/281 of query aligns to 34:243/261 of 1o96A
- binding adenosine monophosphate: D38 (= D38), V63 (≠ M61), V99 (≠ T97), A117 (≠ T119), G118 (= G120), Q120 (= Q122), S121 (≠ T123), Y126 (≠ T128), A127 (= A129), T129 (≠ V131)
- binding flavin-adenine dinucleotide: Q120 (= Q122), Q182 (≠ L184)
Sites not aligning to the query:
P53570 Electron transfer flavoprotein subunit beta; Beta-ETF; Electron transfer flavoprotein small subunit; ETFSS from Methylophilus methylotrophus (Bacterium W3A1) (see paper)
30% identity, 77% coverage: 34:248/281 of query aligns to 35:244/264 of P53570
Sites not aligning to the query:
5ow0B Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
29% identity, 65% coverage: 1:183/281 of query aligns to 1:175/251 of 5ow0B
- binding adenosine monophosphate: L6 (≠ C6), A7 (≠ I7), K8 (= K8), D39 (= D38), Y62 (≠ M61), I95 (≠ L101), A114 (≠ T119), G115 (= G120), Q117 (= Q122), S118 (≠ T123), R123 (≠ T128), G124 (≠ A129), V125 (≠ Q130), V126 (= V131), P127 (≠ G132)
- binding flavin-adenine dinucleotide: L37 (≠ P36), Y38 (= Y37)
Query Sequence
>Dsui_0576 FitnessBrowser__PS:Dsui_0576
MHSVVCIKQVPDSAQIRVHPVTNTIMRQGVPAIINPYDLFALEEALRLKDRFRGTVTVVT
MGPPMAEAALRKCLSYGADDAILVSDRAFAGSDTLATSYALATTIKKIMGNMPVDLIFTG
KQTIDGDTAQVGPGIAKRLDYQLLTYVSKIVDLDLEKKEITVERRAEGGVQLLKTATPCL
ITMLEGTNEMRFGDLDDLFRAARYDLKVWNREAAGIDDAEMIGLKGSPTVVSKVFAPRPR
SQRAEMVETVDNDPKNLAVTLLAKVFTQHPKLEKEIAKRAG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory