SitesBLAST
Comparing Dsui_0664 FitnessBrowser__PS:Dsui_0664 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5i92F Crystal structure of glutamate-1-semialdehyde 2,1- aminomutase (gsa) from pseudomonas aeruginosa
61% identity, 99% coverage: 3:424/427 of query aligns to 2:419/420 of 5i92F
Sites not aligning to the query:
P23893 Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 from Escherichia coli (strain K12) (see paper)
58% identity, 99% coverage: 3:425/427 of query aligns to 2:425/426 of P23893
- K265 (= K266) mutation to R: 2% of wild-type activity.
6w80A Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from stenotrophomonas maltophilia k279a in complex with plp
57% identity, 97% coverage: 3:416/427 of query aligns to 9:419/430 of 6w80A
- active site: V26 (= V20), Y149 (= Y143), D241 (= D238), K269 (= K266)
- binding pyridoxal-5'-phosphate: S121 (= S115), G122 (= G116), T123 (= T117), Y149 (= Y143), H150 (= H144), E208 (= E205), N213 (= N210), D241 (= D238), V243 (= V240), K269 (= K266)
3bs8A Crystal structure of glutamate 1-semialdehyde aminotransferase complexed with pyridoxamine-5'-phosphate from bacillus subtilis (see paper)
53% identity, 99% coverage: 4:425/427 of query aligns to 6:426/430 of 3bs8A
- active site: V22 (= V20), Y145 (= Y143), E207 (= E205), D240 (= D238), M243 (= M241), K268 (= K266), G401 (≠ A400)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G116), T119 (= T117), Y145 (= Y143), H146 (= H144), E207 (= E205), N212 (= N210), D240 (= D238), V242 (= V240), K268 (= K266)
2gsaB Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
53% identity, 99% coverage: 4:424/427 of query aligns to 5:425/427 of 2gsaB
- active site: V21 (= V20), Y144 (= Y143), E206 (= E205), D239 (= D238), M242 (= M241), K267 (= K266), A401 (= A400)
- binding pyridoxal-5'-phosphate: G117 (= G116), T118 (= T117), Y144 (= Y143), E206 (= E205), N211 (= N210), D239 (= D238), V241 (= V240), M242 (= M241), K267 (= K266)
2gsaA Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
53% identity, 99% coverage: 4:424/427 of query aligns to 5:425/427 of 2gsaA
- active site: V21 (= V20), Y144 (= Y143), E206 (= E205), D239 (= D238), M242 (= M241), K267 (= K266), A401 (= A400)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G116), T118 (= T117), Y144 (= Y143), H145 (= H144), G146 (= G145), N211 (= N210), D239 (= D238), V241 (= V240), K267 (= K266)
3usfA Crystal structure of dava-4
52% identity, 99% coverage: 4:424/427 of query aligns to 5:425/427 of 3usfA
- active site: V21 (= V20), Y144 (= Y143), D239 (= D238), M242 (= M241), K267 (= K266), A401 (= A400)
- binding (4s)-4,5-diaminopentanoic acid: S23 (= S22), V25 (= V24), S157 (= S156), K267 (= K266), E400 (= E399)
- binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G117 (= G116), T118 (= T117), Y144 (= Y143), N211 (= N210), D239 (= D238), V241 (= V240), K267 (= K266)
3fq7A Gabaculine complex of gsam (see paper)
52% identity, 99% coverage: 4:424/427 of query aligns to 5:425/427 of 3fq7A
- active site: V21 (= V20), Y144 (= Y143), D239 (= D238), M242 (= M241), K267 (= K266), A401 (= A400)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: S23 (= S22), V25 (= V24), W61 (= W60), G117 (= G116), T118 (= T117), Y144 (= Y143), H145 (= H144), E206 (= E205), N211 (= N210), D239 (= D238), V241 (= V240), M242 (= M241), K267 (= K266), G298 (= G297), T299 (= T298), E400 (= E399)
2hp2A Inter-subunit signaling in gsam (see paper)
52% identity, 99% coverage: 4:424/427 of query aligns to 5:425/427 of 2hp2A
- active site: V21 (= V20), Y144 (= Y143), D239 (= D238), M242 (= M241), K267 (= K266), A401 (= A400)
- binding (4s)-4,5-diaminopentanoic acid: G298 (= G297), T299 (= T298)
- binding (4r)-5-amino-4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S22), G117 (= G116), T118 (= T117), Y144 (= Y143), H145 (= H144), E206 (= E205), N211 (= N210), D239 (= D238), V241 (= V240), M242 (= M241), K267 (= K266)
- binding pyridoxal-5'-phosphate: G298 (= G297), T299 (= T298)
2hp1A Inter-subunit signaling in gsam (see paper)
52% identity, 99% coverage: 4:424/427 of query aligns to 5:425/427 of 2hp1A
- active site: V21 (= V20), Y144 (= Y143), D239 (= D238), M242 (= M241), K267 (= K266), A401 (= A400)
- binding (4s)-4-amino-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S22), V25 (= V24), W61 (= W60), S116 (= S115), G117 (= G116), T118 (= T117), Y144 (= Y143), H145 (= H144), G146 (= G145), E206 (= E205), N211 (= N210), D239 (= D238), V241 (= V240), M242 (= M241), K267 (= K266), E400 (= E399)
2hozA Inter-subunit signaling in gsam (see paper)
52% identity, 99% coverage: 4:424/427 of query aligns to 5:425/427 of 2hozA
- active site: V21 (= V20), Y144 (= Y143), D239 (= D238), M242 (= M241), K267 (= K266), A401 (= A400)
- binding (4s)-4,5-diaminopentanoic acid: E141 (= E140), G156 (= G155), S157 (= S156), P182 (≠ E181), N368 (≠ S367), E370 (≠ A369), K373 (≠ M372)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G116), T118 (= T117), Y144 (= Y143), H145 (= H144), G146 (= G145), E206 (= E205), N211 (= N210), D239 (= D238), G298 (= G297), T299 (= T298)
3fqaA Gabaculien complex of gabaculine resistant gsam version (see paper)
52% identity, 99% coverage: 4:424/427 of query aligns to 4:424/426 of 3fqaA
- active site: V20 (= V20), Y143 (= Y143), D238 (= D238), I241 (≠ M241), K266 (= K266), A400 (= A400)
- binding 3-aminobenzoic acid: S22 (= S22), R25 (= R25), W60 (= W60)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G116 (= G116), T117 (= T117), Y143 (= Y143), E205 (= E205), N210 (= N210), D238 (= D238), V240 (= V240), I241 (≠ M241)
Q42522 Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic; GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
53% identity, 99% coverage: 4:425/427 of query aligns to 50:471/472 of Q42522
- R92 (≠ W46) mutation to K: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with S-162.
- G162 (= G116) mutation to S: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with K-92.
P42799 Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic; AtGSA1; GSA 1; Glutamate-1-semialdehyde aminotransferase 1; GSA-AT 1; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
52% identity, 99% coverage: 4:425/427 of query aligns to 52:473/474 of P42799
- K314 (= K266) modified: N6-(pyridoxal phosphate)lysine
5hdmB Crystal structure of arabidopsis thaliana glutamate-1-semialdehyde-2, 1-aminomutase (see paper)
52% identity, 99% coverage: 4:425/427 of query aligns to 6:427/428 of 5hdmB
- active site: V22 (= V20), Y145 (= Y143), E207 (= E205), D240 (= D238), M243 (= M241), K268 (= K266), A402 (= A400)
- binding pyridoxal-5'-phosphate: G118 (= G116), T119 (= T117), Y145 (= Y143), E207 (= E205), N212 (= N210), D240 (= D238), V242 (= V240), M243 (= M241), K268 (= K266)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G116), T119 (= T117), Y145 (= Y143), E207 (= E205), N212 (= N210), D240 (= D238), V242 (= V240), M243 (= M241), K268 (= K266)
5hdmA Crystal structure of arabidopsis thaliana glutamate-1-semialdehyde-2, 1-aminomutase (see paper)
52% identity, 99% coverage: 4:425/427 of query aligns to 6:427/428 of 5hdmA
- active site: V22 (= V20), Y145 (= Y143), E207 (= E205), D240 (= D238), M243 (= M241), K268 (= K266), A402 (= A400)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G116), T119 (= T117), Y145 (= Y143), G147 (= G145), E207 (= E205), N212 (= N210), D240 (= D238), V242 (= V240), K268 (= K266)
2e7uA Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from thermus thermophilus hb8
54% identity, 97% coverage: 5:418/427 of query aligns to 6:419/424 of 2e7uA
- active site: V21 (= V20), Y144 (= Y143), E206 (= E205), D238 (= D238), M241 (= M241), K266 (= K266), A401 (= A400)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G116), T118 (= T117), Y144 (= Y143), H145 (= H144), N211 (= N210), D238 (= D238), V240 (= V240)
3k28A Crystal structure of a glutamate-1-semialdehyde aminotransferase from bacillus anthracis with bound pyridoxal 5'phosphate
53% identity, 94% coverage: 9:411/427 of query aligns to 11:407/423 of 3k28A
- active site: V22 (= V20), Y145 (= Y143), E202 (= E205), D235 (= D238), M238 (= M241), K263 (= K266), G396 (≠ A400)
- binding calcium ion: I103 (≠ C101), V106 (≠ L104), P107 (= P105), I109 (≠ L107)
- binding pyridoxal-5'-phosphate: G118 (= G116), T119 (= T117), Y145 (= Y143), H146 (= H144), G147 (= G145), E202 (= E205), D235 (= D238), V237 (= V240), M238 (= M241), K263 (= K266)
3usfB Crystal structure of dava-4
49% identity, 99% coverage: 4:424/427 of query aligns to 5:400/402 of 3usfB
- active site: V21 (= V20), Y144 (= Y143), E181 (= E205), D214 (= D238), M217 (= M241), K242 (= K266), A376 (= A400)
- binding (4s)-4,5-diaminopentanoic acid: G273 (= G297), T274 (= T298)
- binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G117 (= G116), T118 (= T117), Y144 (= Y143), E181 (= E205), N186 (= N210), D214 (= D238), V216 (= V240), M217 (= M241), K242 (= K266)
2hp1B Inter-subunit signaling in gsam (see paper)
49% identity, 99% coverage: 4:424/427 of query aligns to 5:396/398 of 2hp1B
- active site: V21 (= V20), Y144 (= Y143), E177 (= E205), D210 (= D238), M213 (= M241), K238 (= K266), A372 (= A400)
- binding (4s)-4-amino-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: G269 (= G297), T270 (= T298)
- binding (4s)-4,5-diaminopentanoic acid: S23 (= S22), V25 (= V24), R26 (= R25), W61 (= W60), Y144 (= Y143)
- binding pyridoxal-5'-phosphate: S116 (= S115), G117 (= G116), T118 (= T117), Y144 (= Y143), E177 (= E205), N182 (= N210), D210 (= D238), V212 (= V240), M213 (= M241), K238 (= K266)
Query Sequence
>Dsui_0664 FitnessBrowser__PS:Dsui_0664
MSSRNQQLFDAAQRHIPGGVNSPVRAFRSVGGAPRFFTRGEGPRVWDAEGKSYLDYVGSW
GPLILGHAHAPTVKAVQEAAALGLSFGAPTEAEIEIADLLCDILPSLDMVRLVSSGTEAT
MSAIRLARGHTGRDLLVKFEGCYHGHSDSLLVKAGSGLLTFGNPSSGGVPADVAKHTLVL
EYNNAEQLAEAFAKQGSEIAAVIVEPVAGNMNLIAPKPEFMQAMRELCSKHGAVLIFDEV
MTGFRVGPQCAQGLFGITPDLTTLGKVIGGGMPVAAFGGKREIMEKIAPLGPVYQAGTLS
GNPVAVAAGLVTLKATRAPGFYDSLAARTKQLTDGLTAAAKKHGVTFCAQSVGGMFGLYF
SATPPTSFAEVMQCDKEAFNRFFHAMLEAGHYLAPSAFEAGFVSAAHTEADIAATIAAAE
AIFAKGV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory