SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Dsui_2068 Dsui_2068 ABC-type metal ion transport system, ATPase component to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
52% identity, 85% coverage: 11:247/278 of query aligns to 2:238/343 of P30750

query
sites
P30750

L

I

A

K

L

L

R

S

G

N

V

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T

K

K

S

V

F

F

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H

T

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A

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L

H

N

P

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S

L

L

D

H

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A

P

P

A

G

G

E

Q

I

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Y

G

G

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I

G

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F

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S

G
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G

A
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A

G
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G

K
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K

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S

T
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T

L

L

L

I

R

R

L

C

A

V

N

N

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L

E

E

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R

P

P

D

T

A

E

G

G

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S

V

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V

L

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H

D

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G

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Q

D

E

L

L

M

T

T

T

L

L

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P

E

A

S

D

E

L

L

R

T

T

K

A

A

R

R

Q

R

R

Q

I

I

G

G

M

M

I

I

F

F

Q

Q

H

H

F

F

N

N

L

L

H

S

N

S

R

R

T

T

V

V

A

F

D

G

N

N

V

V

A

A

F

L

P

P

L

L

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E

I

L

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D

G

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C

L

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F

L

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G

G

L

L

S

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E

D

K

K

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Y

Y

P

P

A

S

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N

L

L

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S

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G

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K

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Q

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V

A

A

I

I

A

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A

A

L

L

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A

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E

N

P

P

H

K

V

V

L

L

A

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E
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E

P

A

T

T

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S

A

A

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L

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D

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S

L

I

L

L

E

E

V

L

L

L

A

K

D

D

V

I

N

N

R

R

L

L

G

G

V

L

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T

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I

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L

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H

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E

M

M

G

D

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V

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L

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C

C

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C

V

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A

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M

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Q

Q

40:46 (vs. 49:55, 100% identical) binding
E166 (= E175) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
52% identity, 85% coverage: 11:247/278 of query aligns to 3:239/344 of 3tuzC

query
sites
3tuzC

L

I

A

K

L

L

R

S

G

N

V

I

S

T

K

K

S

V

F
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F

R

H

T
x
Q

P

G

D

T

G

R

Q

T

Q

I

V

Q

G
x
A

V

L

H

N

P

N

T

V

D

S

L

L

D

H

V

V

A

P

P

A

G

G

E

Q

I

I

H

Y

G

G

I

V

I

I

G

G

F

A

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S

G
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G

A
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A

G
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G

K
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K

S
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S

T
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T

L

L

L

I

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R

L

C

A

V

N

N

L

L

E

E

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R

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P

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E

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G

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S

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V

L

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V

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D

G

G

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Q

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E

L

L

M

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T

T

L

L

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S

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E

A

S

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E

L

L

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T

K

A

A

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R

Q

R

R

Q

I

I

G

G

M

M

I

I

F

F

Q

Q

H

H

F

F

N

N

L

L

H

S

N

S

R

R

T

T

V

V

A

F

D

G

N

N

V

V

A

A

F

L

P

P

L

L

R

E

I

L

A

D

G

N

A

T

D

P

E

K

A

D

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E

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V

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T

E

C

L

L

L

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F

L

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V

G

G

L

L

S

G

E

D

K

K

A

H

G

D

V

S

Y

Y

P

P

A

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N

L

L

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S

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G

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Q

K

K

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Q

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V

A

A

I

I

A

A

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R

A

A

L

L

A

A

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E

N

P

P

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K

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V

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L

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S

A

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L

L

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P

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T

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S

L

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L

L

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L

L

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K

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D

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I

N

N

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L

L

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G

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T

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I

L

L

V

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S

T

H

H

E

E

M

M

G

D

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C

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M

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Q

binding adenosine-5'-diphosphate: F12 (= F20), Q14 (≠ T22), A21 (≠ G29), G42 (= G50), A43 (= A51), G44 (= G52), K45 (= K53), S46 (= S54), T47 (= T55)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 312, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
52% identity, 85% coverage: 11:247/278 of query aligns to 3:239/344 of 3tuiC

query
sites
3tuiC

L

I

A

K

L

L

R

S

G

N

V

I

S

T

K

K

S

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F
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F

R

H

T
x
Q

P

G

D

T

G

R

Q

T

Q

I

V

Q

G

A

V

L

H

N

P

N

T

V

D

S

L

L

D

H

V

V

A

P

P

A

G

G

E

Q

I

I

H

Y

G

G

I

V

I

I

G

G

F

A

S
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S

G
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G

A
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A

G
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G

K
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K

S
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S

T
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T

L

L

L

I

R

R

L

C

A

V

N

N

L

L

E

E

R

R

P

P

D

T

A

E

G

G

Q

S

V

V

V

L

V

V

H

D

G

G

Q

Q

D

E

L

L

M

T

T

T

L

L

S

S

P

E

A

S

D

E

L

L

R

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T

K

A

A

R

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Q

R

R

Q

I

I

G

G

M

M

I

I

F

F

Q

Q

H

H

F

F

N

N

L

L

H

S

N

S

R

R

T

T

V

V

A

F

D

G

N

N

V

V

A

A

F

L

P

P

L

L

R

E

I

L

A

D

G

N

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T

D

P

E

K

A

D

R

E

I

V

N

K

E

R

R

R

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V

K

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T

E

C

L

L

L

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S

F

L

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V

G

G

L

L

S

G

E

D

K

K

A

H

G

D

V

S

Y

Y

P

P

A

S

Q

N

L

L

S

S

G

G

Q

Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

P

S

E

N

P

P

H

K

V

V

L

L

A

C

D

D

E

Q

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A

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S

A

A

L

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D

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P

R

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T

Q

R

S

S

L

I

L

L

E

E

V

L

L

L

A

K

D

D

V

I

N

N

R

R

L

L

G

G

V

L

T

T

I

I

L

L

V

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S

T

H

H

E

E

M

M

G

D

V

V

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V

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K

R

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L

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C

C

H

D

R

C

V

V

S

A

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M

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G

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E

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L

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E

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L

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T

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P

L

L

A

A

Q

Q

binding adenosine-5'-diphosphate: F12 (= F20), Q14 (≠ T22), S41 (= S49), G42 (= G50), A43 (= A51), G44 (= G52), K45 (= K53), S46 (= S54), T47 (= T55)

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
52% identity, 85% coverage: 11:247/278 of query aligns to 3:239/344 of 6cvlD

query
sites
6cvlD

L

I

A

K

L

L

R

S

G

N

V

I

S

T

K

K

S

V

F
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F

R

H

T
x
Q

P

G

D

T

G

R

Q

T

Q
x
I

V

Q

G

A

V

L

H

N

P

N

T

V

D

S

L

L

D

H

V

V

A

P

P

A

G

G

E

Q

I

I

H

Y

G

G

I

V

I

I

G

G

F

A

S
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S

G
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G

A
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A

G
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G

K
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K

S
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S

T
|
T

L

L

L

I

R

R

L

C

A

V

N

N

L

L

E

E

R

R

P

P

D

T

A

E

G

G

Q

S

V

V

V

L

V

V

H

D

G

G

Q

Q

D

E

L

L

M

T

T

T

L

L

S

S

P

E

A

S

D

E

L

L

R

T

T

K

A

A

R

R

Q

R

R

Q

I

I

G

G

M

M

I

I

F

F

Q

Q

H

H

F

F

N

N

L

L

H

S

N

S

R

R

T

T

V

V

A

F

D

G

N

N

V

V

A

A

F

L

P

P

L

L

R

E

I

L

A

D

G

N

A

T

D

P

E

K

A

D

R

E

I

V

N

K

E

R

R

R

V

V

K

T

T

E

C

L

L

L

E

S

F

L

V

V

G

G

L

L

S

G

E

D

K

K

A

H

G

D

V

S

Y

Y

P

P

A

S

Q
x
N

L
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L

S
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S

G
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G

Q
|
Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

P

S

E

N

P

P

H

K

V

V

L

L

A

C

D

D

E

Q

P

A

T

T

S

S

A

A

L

L

D

D

P

P

R

A

T

T

Q

R

S

S

L

I

L

L

E

E

V

L

L

L

A

K

D

D

V

I

N

N

R

R

L

L

G

G

V

L

T

T

I

I

L

L

V

I

S

T

H
|
H

E

E

M

M

G

D

V

V

I

V

R

K

R

R

L

I

C

C

H

D

R

C

V

V

S

A

V

V

M

I

E

S

A

N

G

G

Q

E

I

L

V

I

E

E

R

Q

L

D

T

T

I

V

A

S

N

E

G

V

R

F

I

S

P

H

P

P

D

K

S

T

Q

P

L

L

A

A

Q

Q

binding phosphothiophosphoric acid-adenylate ester: F12 (= F20), Q14 (≠ T22), I19 (≠ Q27), S41 (= S49), G42 (= G50), A43 (= A51), G44 (= G52), K45 (= K53), S46 (= S54), T47 (= T55), N141 (≠ Q149), L142 (= L150), S143 (= S151), G144 (= G152), G145 (= G153), Q146 (= Q154), H200 (= H208)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
47% identity, 80% coverage: 9:230/278 of query aligns to 1:216/241 of 4u00A

query
sites
4u00A

P

P

A

I

L

I

A

R

L

I

R

R

G

N

V

L

S

H

K

K

S

W

F
|
F

R

-

T

G

P

P

D
x
L

G

H

Q
x
V

Q

L

V

K

G

G

V

I

H

H

P

-

T

-

D

-

L

L

D

E

V

V

A

A

P

P

G

G

E

E

I

K

H

L

G

V

I

I

G

G

F

P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

L

T

A

I

N

N

L

R

L

L

E

E

R

D

P

F

D

Q

A

E

G

G

Q

E

V

V

H

D

G

G

Q

-

D

-

L

-

M

-

T

-

L

L

S

S

P

V

A

K

D

D

-

D

-

R

-

A

L

L

R

R

T

E

A

I

R

R

Q

R

R

E

I

V

G

G

M

M

I

V

F

F

Q

Q

H

Q

F

F

N

N

L

L

L

F

H

P

N

H

R

M

T

T

V

V

A

L

D

E

N

N

V

V

A

T

F

L

-

A

P

P

L

M

R

R

I

V

A

R

G

R

A

W

D

P

E

R

A

E

R

K

I

A

N

E

E

K

R

K

V

A

K

L

T

E

C

L

L

L

E

E

F

R

V

V

G

G

L

I

S

L

E

D

K

Q

A

A

G

R

V

K

Y

Y

P

P

A

A

Q

Q

L

L

S

S

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

P

M

E

E

P

P

H

K

V

I

L

M

L

L

A

F

D

D

E

E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

R

E

T

M

T

V

Q

G

S

E

L

V

L

L

E

D

V

V

L

M

A

R

D

D

V

L

N

A

R

Q

R

G

L

-

G

G

V

M

T

T

I

M

L

V

V

V

S

T

H

H

E

E

M

M

G

G

V

F

I

A

R

R

R

E

L

V

C

A

H

D

R

R

V

V

S

V

V

F

M

M

E

D

A

G

G

G

Q

Q

I

I

V

V

E

E

binding adenosine-5'-diphosphate: F12 (= F20), L15 (≠ D24), V17 (≠ Q26), S37 (= S49), G38 (= G50), S39 (≠ A51), G40 (= G52), K41 (= K53), S42 (= S54), T43 (= T55)

P0A9R7 Cell division ATP-binding protein FtsE from Escherichia coli (strain K12) (see paper)
44% identity, 77% coverage: 16:228/278 of query aligns to 7:215/222 of P0A9R7

query
sites
P0A9R7

V

V

S

S

K

K

S

A

F

Y

R

L

T

-

P

-

D

-

G

G

Q

G

Q

R

V

Q

G

A

V

L

H

Q

P

G

T

V

D

T

L

F

D

H

V

M

A

Q

P

P

G

G

E

E

I

M

H

A

G

F

I

L

I

T

G

G

F

H

S

S

G

G

A

A

G

G

K
|
K

S

S

T

T

L

L

R

K

L

L

A

I

N
x
C

L

G

L

I

E

E

R

R

P

P

D

S

A

A

G

G

Q

K

V

I

V

W

V

F

H

S

G

G

Q

H

D

D

L

I

M

T

T

R

L

L

S

K

P

N

A

R

D

E

L

V

R

P

T

F

A

L

R

R

Q

R

R

Q

I

I

G

G

M

M

I

I

F

F

Q

Q

H

D

F

H

N

H

L

L

H

M

N

D

R

R

T

T

V

V

A

Y

D

D

N

N

V

V

A

A

F

I

P

P

L

L

R

I

I

I

A

A

G

G

A

A

D

S

E

G

A

D

R

D

I

I

N

R

E

R

R

R

V

V

K

S

T

A

C

A

L

L

E

D

F

K

V

V

G

G

L

L

S

L

E

D

K

K

A

A

G

K

V

N

Y

F

P

P

A

I

Q

Q

L

L

S

S

G

G

Q

E

K

Q

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

L

V

A

V

P

N

E

K

P

P

H

A

V

V

L

L

A

A

D

D

E

E

P

P

T

T

S

G

A

N

L

L

D

D

P

D

R

A

T

L

T

S

Q

E

S

G

L

I

L

L

E

R

V

L

L

F

A

E

D

E

V

F

N

N

R

-

R

R

L

V

G

G

V

V

T

T

I

V

L

L

L

M

V

A

S

T

H

H

E

D

M

I

G

N

V

L

I

I

R

S

R

R

L

R

C

S

H

Y

R

R

V

M

S

L

V

T

M

L

E

S

A

D

G

G

Q

H

I

L

K41 (= K53) mutation to R: Does not bind ATP.
C49 (≠ N61) mutation to A: Prevents dimer formation. Does not alter ATP-binding.

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
45% identity, 72% coverage: 35:233/278 of query aligns to 22:219/240 of 4ymuJ

query
sites
4ymuJ

L

L

D

K

V

V

A

N

P

K

G

G

E

E

I

V

H

V

G

V

I

I

G

G

F

P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

L

C

A

I

N

N

L

L

E
|
E

R

E

P

P

D

T

A

K

G

G

Q

E

V

V

V

F

V

I

H

D

G

G

Q

V

D

K

L

I

M

N

T

N

L

-

S

G

P

K

A

V

D

N

L

I

R

N

T

K

A

V

R

R

Q

Q

R

K

I

V

G

G

M

M

I

V

F

F

Q

Q

H

H

F

F

N

N

L

L

L

F

H

P

N

H

R

L

T

T

V

A

A

I

D

E

N

N

V

I

A

T

F

L

-

A

P

P

L

V

R

K

I

V

A

K

G

K

A

M

D

N

E

K

A

K

R

E

I

A

N

E

E

E

R

L

V

A

K

V

T

D

C

L

L

L

E

A

F

K

V

V

G

G

L

L

S

L

E

D

K

K

A

K

G

D

V

Q

Y

Y

P

P

A

I

Q

K

L

L

S

S

G

G

Q

Q

K

K

Q

Q

R

R

V

L

A

A

I

I

A

A

R

R

A

A

L

L

A

A

P

M

E

Q

P

P

H

E

V

V

L

M

L

L

A

F

D

D

E
|
E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

R

E

T

M

T

V

Q

K

S

E

L

V

L

L

E

N

V

V

L

M

A

K

D

Q

V

L

N

A

R

N

R

E

L

-

G

G

V

M

T

T

I

M

L

V

V

V

S

T

H
|
H

E

E

M

M

G

G

V

F

I

A

R

R

R

E

L

V

C

G

H

D

R

R

V

V

S

I

V

F

M

M

E

D

A

D

G

G

Q

V

I

I

V

V

E

E

R

E

L

G

T

T

binding adenosine-5'-triphosphate: S36 (= S49), G37 (= G50), S38 (≠ A51), G39 (= G52), K40 (= K53), S41 (= S54), T42 (= T55), E51 (= E64), E162 (= E175), H194 (= H208)
binding magnesium ion: S41 (= S54), E162 (= E175)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11, 14, 16

3c4jA Abc protein artp in complex with atp-gamma-s
42% identity, 71% coverage: 34:230/278 of query aligns to 23:218/242 of 3c4jA

query
sites
3c4jA

D

N

L

V

D

H

V

I

A

R

P

E

G

G

E

E

I

V

H

V

G

V

I

V

I

I

G

G

F
x
P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

L

C

A

L

N

N

L

L

E

E

R

D

P

F

D

D

A

E

G

G

Q

E

V

I

H

D

G

G

Q

I

D

N

L

L

M

K

T

A

L

-

S

K

P

D

A

T

D

N

L

L

R

N

T

K

A

V

R

R

Q

E

R

E

I

V

G

G

M

M

I

V

F

F

Q

Q

H

R

F

F

N

N

L

L

L

F

H

P

N

H

R

M

T

T

V

V

A

L

D

N

N

N

V

I

A

T

F

L

-

A

P

P

L

M

R

K

I

V

A

R

G

K

A

W

D

P

E

R

A

E

R

K

I

A

N

E

E

A

R

K

V

A

K

M

T

E

C

L

L

L

E

D

F

K

V

V

G

G

L

L

S

K

E

D

K

K

A

A

G
x
H

V

A

Y
|
Y

P

P

A

D

Q

S

L

L

S

S

G

G

Q

Q

K

A

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

P

M

E

E

P

P

H

K

V

I

L

M

L

L

A

F

D

D

E
|
E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

R

E

T

M

T

V

Q

G

S

E

L

V

L

L

E

S

V

V

L

M

A

K

D

Q

V

L

N

A

R

N

R

E

L

-

G

G

V

M

T

T

I

M

L

V

V

V

S

T

H

H

E

E

M

M

G

G

V

F

I

A

R

R

R

E

L

V

C

G

H

D

R

R

V

V

S

L

V

F

M

M

E

D

A

G

G

G

Q

Y

I

I

V

I

E

E

binding phosphothiophosphoric acid-adenylate ester: P37 (≠ F48), S38 (= S49), G39 (= G50), S40 (≠ A51), G41 (= G52), K42 (= K53), S43 (= S54), T44 (= T55), H133 (≠ G144), Y135 (= Y146), E164 (= E175)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 13, 18

3c41J Abc protein artp in complex with amp-pnp/mg2+
42% identity, 71% coverage: 34:230/278 of query aligns to 23:218/242 of 3c41J

query
sites
3c41J

D

N

L

V

D

H

V

I

A

R

P

E

G

G

E

E

I

V

H

V

G

V

I

V

I

I

G

G

F
x
P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R
|
R

L

C

A

L

N

N

L

L

E

E

R

D

P

F

D

D

A

E

G

G

Q

E

V

I

H

D

G

G

Q

I

D

N

L

L

M

K

T

A

L

-

S

K

P

D

A

T

D

N

L

L

R

N

T

K

A

V

R

R

Q

E

R

E

I

V

G

G

M

M

I

V

F

F

Q

Q

H

R

F

F

N

N

L

L

L

F

H

P

N

H

R

M

T

T

V

V

A

L

D

N

N

N

V

I

A

T

F

L

-

A

P

P

L

M

R

K

I

V

A

R

G

K

A

W

D

P

E

R

A

E

R

K

I

A

N

E

E

A

R

K

V

A

K

M

T

E

C

L

L

L

E

D

F

K

V

V

G

G

L

L

S

K

E

D

K

K

A

A

G
x
H

V

A

Y
|
Y

P

P

A

D

Q

S

L

L

S

S

G

G

Q

Q

K

A

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

P

M

E

E

P

P

H

K

V

I

L

M

L

L

A

F

D
|
D

E

E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

R

E

T

M

T

V

Q

G

S

E

L

V

L

L

E

S

V

V

L

M

A

K

D

Q

V

L

N

A

R

N

R

E

L

-

G

G

V

M

T

T

I

M

L

V

V

V

S

T

H

H

E

E

M

M

G

G

V

F

I

A

R

R

R

E

L

V

C

G

H

D

R

R

V

V

S

L

V

F

M

M

E

D

A

G

G

G

Q

Y

I

I

V

I

E

E

binding phosphoaminophosphonic acid-adenylate ester: P37 (≠ F48), S38 (= S49), G39 (= G50), S40 (≠ A51), G41 (= G52), K42 (= K53), S43 (= S54), T44 (= T55), R47 (= R58), H133 (≠ G144), Y135 (= Y146)
binding magnesium ion: S43 (= S54), D163 (= D174)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13, 18

2olkA Abc protein artp in complex with adp-beta-s
42% identity, 71% coverage: 34:230/278 of query aligns to 23:218/242 of 2olkA

query
sites
2olkA

D

N

L

V

D

H

V

I

A

R

P

E

G

G

E

E

I

V

H

V

G

V

I

V

I

I

G

G

F

P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

L

C

A

L

N

N

L

L

E

E

R

D

P

F

D

D

A

E

G

G

Q

E

V

I

H

D

G

G

Q

I

D

N

L

L

M

K

T

A

L

-

S

K

P

D

A

T

D

N

L

L

R

N

T

K

A

V

R

R

Q

E

R

E

I

V

G

G

M

M

I

V

F

F

Q

Q

H

R

F

F

N

N

L

L

L

F

H

P

N

H

R

M

T

T

V

V

A

L

D

N

N

N

V

I

A

T

F

L

-

A

P

P

L

M

R

K

I

V

A

R

G

K

A

W

D

P

E

R

A

E

R

K

I

A

N

E

E

A

R

K

V

A

K

M

T

E

C

L

L

L

E

D

F

K

V

V

G

G

L

L

S

K

E

D

K

K

A

A

G

H

V

A

Y

Y

P

P

A

D

Q

S

L

L

S

S

G

G

Q

Q

K

A

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

P

M

E

E

P

P

H

K

V

I

L

M

L

L

A

F

D

D

E

E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

R

E

T

M

T

V

Q

G

S

E

L

V

L

L

E

S

V

V

L

M

A

K

D

Q

V

L

N

A

R

N

R

E

L

-

G

G

V

M

T

T

I

M

L

V

V

V

S

T

H

H

E

E

M

M

G

G

V

F

I

A

R

R

R

E

L

V

C

G

H

D

R

R

V

V

S

L

V

F

M

M

E

D

A

G

G

G

Q

Y

I

I

V

I

E

E

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S38 (= S49), G39 (= G50), S40 (≠ A51), G41 (= G52), K42 (= K53), S43 (= S54), T44 (= T55)

Sites not aligning to the query:

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: 13, 16, 18

2oljA Abc protein artp in complex with adp/mg2+
42% identity, 71% coverage: 34:230/278 of query aligns to 23:218/242 of 2oljA

query
sites
2oljA

D

N

L

V

D

H

V

I

A

R

P

E

G

G

E

E

I

V

H

V

G

V

I

V

I

I

G

G

F

P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

L

C

A

L

N

N

L

L

E

E

R

D

P

F

D

D

A

E

G

G

Q

E

V

I

H

D

G

G

Q

I

D

N

L

L

M

K

T

A

L

-

S

K

P

D

A

T

D

N

L

L

R

N

T

K

A

V

R

R

Q

E

R

E

I

V

G

G

M

M

I

V

F

F

Q

Q

H

R

F

F

N

N

L

L

L

F

H

P

N

H

R

M

T

T

V

V

A

L

D

N

N

N

V

I

A

T

F

L

-

A

P

P

L

M

R

K

I

V

A

R

G

K

A

W

D

P

E

R

A

E

R

K

I

A

N

E

E

A

R

K

V

A

K

M

T

E

C

L

L

L

E

D

F

K

V

V

G

G

L

L

S

K

E

D

K

K

A

A

G
x
H

V

A

Y
|
Y

P

P

A

D

Q

S

L

L

S

S

G

G

Q

Q

K

A

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

P

M

E

E

P

P

H

K

V

I

L

M

L

L

A

F

D

D

E

E

P

P

T

T

S

S

A

A

L

L

D

D

P

P

R

E

T

M

T

V

Q

G

S

E

L

V

L

L

E

S

V

V

L

M

A

K

D

Q

V

L

N

A

R

N

R

E

L

-

G

G

V

M

T

T

I

M

L

V

V

V

S

T

H

H

E

E

M

M

G

G

V

F

I

A

R

R

R

E

L

V

C

G

H

D

R

R

V

V

S

L

V

F

M

M

E

D

A

G

G

G

Q

Y

I

I

V

I

E

E

binding adenosine-5'-diphosphate: S38 (= S49), G39 (= G50), S40 (≠ A51), G41 (= G52), K42 (= K53), S43 (= S54), T44 (= T55), H133 (≠ G144), Y135 (= Y146)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 13, 18

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
38% identity, 74% coverage: 25:230/278 of query aligns to 37:243/382 of 7ahhC

query
sites
7ahhC

G

G

Q

A

Q
x
T

V
|
V

G
|
G

V

V

H

Y

P

D

T

T

D

N

L

F

D

E

V

I

A

N

P

E

G

G

E

E

I

I

H

F

G

V

I

I

I

M

G

G

F

L

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

L

L

A

L

N

N

L

R

L

L

E

I

R

E

P

P

D

T

A

S

G

G

Q

K

V

I

V

F

V

I

H

D

G

N

Q

Q

D

D

L

V

M

A

T

T

L

L

S

N

P

K

A

E

D

D

L

L

R

L

T

Q

A

V

R

R

Q

R

R

K

-

T

I

M

G

S

M

M

I

V

F

F

Q

Q

H

N

F

F

N

G

L

L

L

F

H

P

N

H

R

R

T

T

V

I

A

L

D

E

N

N

V

T

A

E

F

Y

P

G

L

L

R

E

I

V

A

Q

G

N

A

V

D

P

E

K

A

E

R

E

I

R

N

R

E

K

R

R

V

A

K

E

T

K

C

A

L

L

E

D

F

N

V

A

G

N

L

L

S

L

E

D

K

F

A

K

G

D

V

Q

Y

Y

P

P

A

K

Q

Q

L

L

S

S

G

G

Q

M

K

Q

Q

Q

R

R

V

V

A

G

I

L

A

A

R

R

A

A

L

L

A

A

P

N

E

D

P

P

H

E

V

I

L

L

A

M

D
|
D

E
|
E

P

A

T

F

S

S

A

A

L

L

D

D

P

P

R

L

T

I

T

R

Q

R

S

E

L

M

L

Q

E

D

V

E

L

L

A

L

D

E

V

L

N

Q

R

A

R

K

L

F

G

Q

V

K

T

T

I

I

L

I

L

F

V

V

S

S

H

H

E

D

M

L

G

N

V

E

I

A

R

L

R

R

L

I

C

G

H

D

R

R

V

I

S

A

V

I

M

M

E

K

A

D

G

G

Q

K

I

I

V

M

E

Q

binding phosphoaminophosphonic acid-adenylate ester: T39 (≠ Q27), V40 (= V28), G41 (= G29), S61 (= S49), G62 (= G50), S63 (≠ A51), G64 (= G52), K65 (= K53), S66 (= S54), T67 (= T55), D187 (= D174), E188 (= E175)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
38% identity, 74% coverage: 25:230/278 of query aligns to 37:243/382 of 7aheC

query
sites
7aheC

G

G

Q

A

Q

T

V

V

G

G

V

V

H

Y

P

D

T

T

D

N

L

F

D

E

V

I

A

N

P

E

G

G

E

E

I

I

H

F

G

V

I

I

I

M

G

G

F

L

S

S

G

G

A

S

G

G

K

K

S

S

T

T

L

L

R

R

L

L

A

L

N

N

L

R

L

L

E

I

R

E

P

P

D

T

A

S

G

G

Q

K

V

I

V

F

V

I

H

D

G

N

Q

Q

D

D

L

V

M

A

T

T

L

L

S

N

P

K

A

E

D

D

L

L

R

L

T

Q

A

V

R

R

Q

R

R

K

-

T

I

M

G

S

M

M

I

V

F

F

Q

Q

H

N

F

F

N

G

L

L

L

F

H

P

N

H

R

R

T

T

V

I

A

L

D

E

N

N

V

T

A

E

F

Y

P

G

L

L

R

E

I

V

A

Q

G

N

A

V

D

P

E

K

A

E

R

E

I

R

N

R

E

K

R

R

V

A

K

E

T

K

C

A

L

L

E

D

F

N

V

A

G

N

L

L

S

L

E

D

K

F

A

K

G

D

V

Q

Y

Y

P

P

A

K

Q

Q

L

L

S

S

G

G

Q

M

K

Q

Q

Q

R

R

V

V

A

G

I

L

A

A

R

R

A

A

L

L

A

A

P

N

E

D

P

P

H

E

V

I

L

L

A

M

D

D

E

E

P

A

T

F

S

S

A

A

L

L

D

D

P

P

R

L

T

I

T

R

Q

R

S

E

L

M

L

Q

E

D

V

E

L

L

A

L

D

E

V

L

N

Q

R

A

R

K

L

F

G

Q

V

K

T

T

I

I

L

I

L

F

V

V

S

S

H

H

E

D

M

L

G

N

V

E

I

A

R

L

R

R

L

I

C

G

H

D

R

R

V

I

S

A

V

I

M

M

E

K

A

D

G

G

Q

K

I

I

V

M

E

Q

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 295, 296, 297, 298, 357, 372

7ahdC Opua (e190q) occluded (see paper)
38% identity, 74% coverage: 25:230/278 of query aligns to 37:243/260 of 7ahdC

query
sites
7ahdC

G

G

Q

A

Q
x
T

V

V

G

G

V

V

H

Y

P

D

T

T

D

N

L

F

D

E

V

I

A

N

P

E

G

G

E

E

I

I

H

F

G

V

I

I

I

M

G

G

F

L

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

L

L

A

L

N

N

L

R

L

L

E

I

R

E

P

P

D

T

A

S

G

G

Q

K

V

I

V

F

V

I

H

D

G

N

Q

Q

D

D

L

V

M

A

T

T

L

L

S

N

P

K

A

E

D

D

L

L

R

L

T

Q

A

V

R

R

Q

R

R

K

-

T

I

M

G

S

M

M

I

V

F

F

Q
|
Q

H

N

F

F

N

G

L

L

L

F

H

P

N

H

R

R

T

T

V

I

A

L

D

E

N

N

V

T

A

E

F

Y

P

G

L

L

R

E

I

V

A

Q

G

N

A

V

D

P

E

K

A

E

R

E

I

R

N

R

E

K

R

R

V

A

K

E

T

K

C

A

L

L

E

D

F

N

V

A

G

N

L

L

S

L

E

D

K

F

A

K

G

D

V

Q

Y

Y

P

P

A
x
K

Q
|
Q

L

L

S
|
S

G

G

G
|
G

Q
x
M

K

Q

Q

Q

R

R

V

V

A

G

I

L

A

A

R

R

A

A

L

L

A

A

P

N

E

D

P

P

H

E

V

I

L

L

A

M

D

D

E
x
Q

P

A

T

F

S

S

A

A

L

L

D

D

P

P

R

L

T

I

T

R

Q

R

S

E

L

M

L

Q

E

D

V

E

L

L

A

L

D

E

V

L

N

Q

R

A

R

K

L

F

G

Q

V

K

T

T

I

I

L

I

L

F

V

V

S

S

H
|
H

E

D

M

L

G

N

V

E

I

A

R

L

R

R

L

I

C

G

H

D

R

R

V

I

S

A

V

I

M

M

E

K

A

D

G

G

Q

K

I

I

V

M

E

Q

binding adenosine-5'-triphosphate: T39 (≠ Q27), S61 (= S49), G62 (= G50), S63 (≠ A51), G64 (= G52), K65 (= K53), S66 (= S54), T67 (= T55), Q111 (= Q98), K161 (≠ A148), Q162 (= Q149), S164 (= S151), G166 (= G153), M167 (≠ Q154), Q188 (≠ E175), H221 (= H208)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 29

6z4wA Ftse structure from streptococcus pneumoniae in complex with adp (space group p 1) (see paper)
37% identity, 82% coverage: 2:229/278 of query aligns to 9:218/230 of 6z4wA

query
sites
6z4wA

T

V

Q

K

N

K

T
x
Y

D

D

S
x
N

P

G

A
x
T

L

T

A
|
A

L

L

R

R

G

G

V

V

S

S

K

V

S

S

F

-

R

-

T

-

P

-

D

-

G

-

Q

-

V

-

G

-

V

-

H

-

P

-

T

-

D

-

L

-

D

-

V

V

A

Q

P

P

G

G

E

E

I

F

H

A

G

Y

I

I

I

V

G

G

F

P

S
|
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

I

R

R

L

S

A

L

N

Y

L

R

L

E

E

V

R

K

P

I

D

D

A

K

G

G

Q

S

V

L

V

S

V

V

H

A

G

G

Q

F

D

N

L

L

M

V

T

K

L

I

S

K

P

K

A

K

D

D

L

V

R

P

T

L

A

L

R

R

Q

R

R

S

I

V

G

G

M

V

I

V

F

F

Q

Q

H

D

F

Y

N

K

L

L

H

P

N

K

R

K

T

T

V

V

A

Y

D

E

N

N

V

I

A

A

F

Y

P

A

L

M

R

E

I

V

A

I

G

G

A

E

D

N

E

R

A

R

R

N

I

I

N

K

E

R

R

R

V

V

K

M

T

E

C

V

L

L

E

D

F

L

V

V

G

G

L

L

S

K

E

H

K

K

A

V

G

R

V

S

Y

F

P

P

A

N

Q

E

L

L

S

S

G

G

Q

E

K

Q

Q

Q

R

R

V

I

A

A

I

I

A

A

R

R

A

A

L

I

A

V

P

N

E

N

P

P

H

K

V

V

L

L

L

I

A

A

D

D

E

E

P

P

T

T

S

G

A

N

L

L

D

D

P

P

R

D

T

N

T

S

Q

W

S

E

L

I

L

M

E

N

V

L

L

L

A

E

D

R

V

I

N

N

R

L

R

Q

L

-

G

G

V

T

T

T

I

I

L

L

L

M

V

A

S

T

H

H

E

N

M

S

G

Q

V

I

I

V

R

N

R

T

L

L

C

R

H

H

R

R

V

V

S

I

V

A

M

I

E

E

A

N

G

G

Q

R

I

V

V

V

binding adenosine-5'-diphosphate: Y13 (≠ T6), N15 (≠ S8), T17 (≠ A10), A19 (= A12), S39 (= S49), G40 (= G50), A41 (= A51), G42 (= G52), K43 (= K53), S44 (= S54), T45 (= T55)

6z67B Ftse structure of streptococcus pneumoniae in complex with amppnp at 2.4 a resolution (see paper)
37% identity, 82% coverage: 2:229/278 of query aligns to 9:218/229 of 6z67B

query
sites
6z67B

T

V

Q

K

N

K

T
x
Y

D

D

S

N

P
x
G

A
x
T

L

T

A
|
A

L

L

R

R

G

G

V

V

S

S

K

V

S

S

F

-

R

-

T

-

P

-

D

-

G

-

Q

-

V

-

G

-

V

-

H

-

P

-

T

-

D

-

L

-

D

-

V

V

A

Q

P

P

G

G

E

E

I

F

H

A

G

Y

I

I

I

V

G

G

F

P

S
|
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

I

R

R

L

S

A

L

N

Y

L

R

L

E

E

V

R

K

P

I

D

D

A

K

G

G

Q

S

V

L

V

S

V

V

H

A

G

G

Q

F

D

N

L

L

M

V

T

K

L

I

S

K

P

K

A

K

D

D

L

V

R

P

T

L

A

L

R

R

Q

R

R

S

I

V

G

G

M

V

I

V

F

F

Q

Q

H

D

F

Y

N

K

L

L

H

P

N

K

R

K

T

T

V

V

A

Y

D

E

N

N

V

I

A

A

F

Y

P

A

L

M

R

E

I

V

A

I

G

G

A

E

D

N

E

R

A

R

R

N

I

I

N

K

E

R

R

R

V

V

K

M

T

E

C

V

L

L

E

D

F

L

V

V

G

G

L

L

S

K

E

H

K

K

A

V

G

R

V

S

Y

F

P

P

A

N

Q

E

L

L

S

S

G

G

Q

E

K

Q

Q

Q

R

R

V

I

A

A

I

I

A

A

R

R

A

A

L

I

A

V

P

N

E

N

P

P

H

K

V

V

L

L

L

I

A

A

D

D

E

E

P

P

T

T

S

G

A

N

L

L

D

D

P

P

R

D

T

N

T

S

Q

W

S

E

L

I

L

M

E

N

V

L

L

L

A

E

D

R

V

I

N

N

R

L

R

Q

L

-

G

G

V

T

T

T

I

I

L

L

L

M

V

A

S

T

H
|
H

E

N

M

S

G

Q

V

I

I

V

R

N

R

T

L

L

C

R

H

H

R

R

V

V

S

I

V

A

M

I

E

E

A

N

G

G

Q

R

I

V

V

V

binding phosphoaminophosphonic acid-adenylate ester: Y13 (≠ T6), G16 (≠ P9), T17 (≠ A10), A19 (= A12), S39 (= S49), G40 (= G50), A41 (= A51), G42 (= G52), K43 (= K53), S44 (= S54), T45 (= T55), H197 (= H208)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
39% identity, 79% coverage: 16:234/278 of query aligns to 11:229/233 of P75957

query
sites
P75957

V

L

S

C

K

K

S

R

F

Y

R

Q

T

E

P

G

D

S

G

V

Q

Q

Q

T

V

D

G

V

V

L

H

H

P

N

T

V

D

S

L

F

D

S

V

V

A

G

P

E

G

G

E

E

I

M

H

M

G

A

I

I

I

V

G
|
G

F

S

S

S

G

G

A

S

G

G

K

K

S

S

T

T

L

L

R

H

L

L

A

L

N

G

L

G

L

L

E

D

R

T

P

P

D

T

A

S

G

G

Q

D

V

V

V

I

V

F

H

N

G

G

Q

Q

D

P

L

M

M

S

T

K

L

L

S

S

-

S

-

A

P

K

A

A

D

E

L

L

R

R

T

N

A

-

R

-

Q

Q

R

K

I

L

G

G

M

F

I

I

F

Y

Q

Q

H

F

F

H

N

H

L

L

H

P

N

D

R

F

T

T

V

A

A

L

D

E

N

N

V

V

A

A

F

M

P

P

L

L

R

L

I

I

A

G

G

K

A

K

D

K

E

P

A

A

R

E

I

I

N

N

E

S

R

R

V

A

K

L

T

E

C

M

L

L

E

K

F

A

V

V

G

G

L

L

S

D

E

H

K

R

A

A

G

N

V

H

Y

R

P

P

A

S

Q

E

L

L

S

S

G

G

Q

E

K

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

V

P

N

E

N

P

P

H

R

V

L

L

V

L

L

A

A

D

D

E

E

P

P

T

T

S

G

A

N

L

L

D

D

P

A

R

R

T

N

T

A

Q

D

S

S

L

I

L

F

E

Q

V

L

L

L

A

G

D

E

V

L

N

N

R

R

R

L

L

Q

G

G

V

T

T

A

I

F

L

L

L

V

V

V

S

T

H

H

E

D

M

L

G

Q

V

L

I

A

R

K

R

R

L

M

C

S

H

R

R

Q

V

L

S

E

V

-

M

M

E

R

A

D

G

G

Q

R

I

L

V

T

E

A

R

E

L

L

T

S

I

L

G42 (= G47) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
39% identity, 79% coverage: 16:234/278 of query aligns to 8:226/226 of 7mdyC

query
sites
7mdyC

V

L

S

C

K

K

S

R

F
x
Y

R

Q

T

E

P

G

D

S

G

V

Q

Q

Q
x
T

V

D

G
x
V

V

L

H

H

P

N

T

V

D

S

L

F

D

S

V

V

A

G

P

E

G

G

E

E

I

M

H

M

G

A

I

I

I

V

G

G

F
x
S

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

H

L

L

A

L

N

G

L

G

L

L

E

D

R

T

P

P

D

T

A

S

G

G

Q

D

V

V

V

I

V

F

H

N

G

G

Q

Q

D

P

L

M

M

S

T

K

L

L

S

S

-

S

-

A

P

K

A

A

D

E

L

L

R

R

T

N

A

-

R

-

Q

Q

R

K

I

L

G

G

M

F

I

I

F

Y

Q
|
Q

H

F

F

H

N

H

L

L

H

P

N

D

R

F

T

T

V

A

A

L

D

E

N

N

V

V

A

A

F

M

P

P

L

L

R

L

I

I

A

G

G

K

A

K

D

K

E

P

A

A

R

E

I

I

N

N

E

S

R

R

V

A

K

L

T

E

C

M

L

L

E

K

F

A

V

V

G

G

L

L

S

D

E

H

K
x
R

A

A

G

N

V
x
H

Y

R

P

P

A

S

Q
x
E

L

L

S
|
S

G
|
G

Q
x
E

K

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

V

P

N

E

N

P

P

H

R

V

L

L

V

L

L

A

A

D

D

E
|
E

P

P

T

T

S

G

A
x
N

L

L

D

D

P

A

R

R

T

N

T

A

Q

D

S

S

L

I

L

F

E

Q

V

L

L

L

A

G

D

E

V

L

N

N

R

R

R

L

L

Q

G

G

V

T

T

A

I

F

L

L

L

V

V

V

S

T

H
|
H

E

D

M

L

G

Q

V

L

I

A

R

K

R

R

L

M

C

S

H

R

R

Q

V

L

S

E

V

-

M

M

E

R

A

D

G

G

Q

R

I

L

V

T

E

A

R

E

L

L

T

S

I

M

binding adp orthovanadate: Y12 (≠ F20), T19 (≠ Q27), V21 (≠ G29), S40 (≠ F48), S41 (= S49), G42 (= G50), S43 (≠ A51), G44 (= G52), K45 (= K53), S46 (= S54), T47 (= T55), Q91 (= Q98), R135 (≠ K142), H138 (≠ V145), E142 (≠ Q149), S144 (= S151), G145 (= G152), G146 (= G153), E147 (≠ Q154), E168 (= E175), N172 (≠ A179), H201 (= H208)
binding magnesium ion: S46 (= S54), Q91 (= Q98)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
40% identity, 72% coverage: 11:209/278 of query aligns to 4:202/226 of 5xu1B

query
sites
5xu1B

L

I

A

S

L

L

R

K

G

N

V

I

S

F

K

R

S

S

F

Y

R

R

T

N

P

G

D

D

G

Q

Q

E

Q

L

V

Q

G

V

V

L

H

K

P

N

T

I

D

N

L

L

D

E

V

V

A

N

P

E

G

G

E

E

I

F

H

V

G

A

I

I

I

M

G

G

F

P

S

S

G

G

A

S

G
|
G

K
|
K

S
|
S

T

T

L

L

L

M

R

N

L

T

A

I

N

G

L

M

L

L

E

D

R

T

P

P

D

T

A

S

G

G

Q

E

V

Y

V

L

H

E

G

G

Q

Q

D

E

L

V

M

A

T

G

L

L

S

G

P

E

A

K

D

Q

L

L

R

A

T

K

A

V

R

R

-

N

Q

Q

R

Q

I

I

G

G

M

F

I

V

F

F

Q

Q

H

Q

F

F

N

F

L

L

H

S

N

K

R

L

T

N

V

A

A

L

D

Q

N

N

V

V

A

E

F

L

P

P

L

L

R

I

I

Y

A

A

G

G

A

V

D

S

E

S

A

S

R

K

I

R

N

R

E

K

R

L

V

A

K

E

T

E

C

Y

L

L

E

D

F

K

V

V

G

E

L

L

S

T

E

E

K

R

A

S

G

H

V

H

Y

L

P

P

A

S

Q

E

L

L

S

S

G

G

Q

Q

K

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

V

P

N

E

N

P

P

H

S

V

I

L

I

L

L

A

A

D

D

E

E

P

P

T

T

S

G

A

A

L

L

D

D

P

T

R

K

T

T

T

G

Q

N

S

Q

L

I

L

M

E

Q

V

L

L

L

A

V

D

D

V

L

N

N

R

K

R

E

L

-

G

G

V

K

T

T

I

I

L

I

L

M

V

V

S

T

H

H

E

E

binding magnesium ion: G45 (= G52), K46 (= K53), S47 (= S54)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs
38% identity, 79% coverage: 16:234/278 of query aligns to 10:228/229 of 7v8iD

query
sites
7v8iD

V

L

S

C

K

K

S

R

F

Y

R

Q

T

E

P

G

D

S

G

V

Q

Q

Q

T

V

D

G
x
V

V

L

H

H

P

N

T

V

D

S

L

F

D

S

V

V

A

G

P

E

G

G

E

E

I

M

H

M

G

A

I

I

I

V

G

G

F

S

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

H

L

L

A

L

N

G

L

G

L

L

E

D

R

T

P

P

D

T

A

S

G

G

Q

D

V

V

V

I

V

F

H

N

G

G

Q

Q

D

P

L

M

M

S

T

K

L

L

S

S

-

S

-

A

P

K

A

A

D

E

L

L

R

R

T

N

A

-

R

-

Q

Q

R

K

I

L

G

G

M

F

I

I

F

Y

Q
|
Q

H

F

F

H

N

H

L

L

H

P

N

D

R

F

T

T

V

A

A

L

D

E

N

N

V

V

A

A

F

M

P

P

L

L

R

L

I

I

A

G

G

K

A

K

D

K

E

P

A

A

R

E

I

I

N

N

E

S

R

R

V

A

K

L

T

E

C

M

L

L

E

K

F

A

V

V

G

G

L

L

S

D

E

H

K
x
R

A

A

G

N

V
x
H

Y

R

P

P

A

S

Q
x
E

L
|
L

S
|
S

G
|
G

Q
x
E

K

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

A

V

P

N

E

N

P

P

H

R

V

L

L

V

L

L

A

A

D

D

E

Q

P

P

T

T

S

G

A
x
N

L

L

D

D

P

A

R

R

T

N

T

A

Q

D

S

S

L

I

L

F

E

Q

V

L

L

L

A

G

D

E

V

L

N

N

R

R

R

L

L

Q

G

G

V

T

T

A

I

F

L

L

L

V

V

V

S

T

H
|
H

E

D

M

L

G

Q

V

L

I

A

R

K

R

R

L

M

C

S

H

R

R

Q

V

L

S

E

V

-

M

M

E

R

A

D

G

G

Q

R

I

L

V

T

E

A

R

E

L

L

T

S

I

L

binding phosphoaminophosphonic acid-adenylate ester: V23 (≠ G29), S43 (= S49), G44 (= G50), S45 (≠ A51), G46 (= G52), K47 (= K53), S48 (= S54), T49 (= T55), Q93 (= Q98), R137 (≠ K142), H140 (≠ V145), E144 (≠ Q149), L145 (= L150), S146 (= S151), G147 (= G152), G148 (= G153), E149 (≠ Q154), N174 (≠ A179), H203 (= H208)
binding magnesium ion: S48 (= S5