SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Dsui_2078 FitnessBrowser__PS:Dsui_2078 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

P00099 Cytochrome c-551; Cytochrome C8; Cytochrome c551 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
57% identity, 95% coverage: 6:102/102 of query aligns to 7:104/104 of P00099

query
sites
P00099

L
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S
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S

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L

A
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x
T

G
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G
x
T

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L

F
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L

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K

23:104 (vs. 21:102, 57% identical) modified: mature protein, Cytochrome c-551
H38 (= H36) binding axial binding residue
M83 (= M81) binding axial binding residue

Sites not aligning to the query:

1:22 signal peptide

5xecC Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
61% identity, 78% coverage: 23:102/102 of query aligns to 1:80/80 of 5xecC

query
sites
5xecC

D

N

E

E

A

Q

L

L

A

A

K

K

A

Q

K

K

N

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C

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C

H
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H

Q

D

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A

K

K

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L

V

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G

P
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P

A

A

Y
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Y

K

K

D

D

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A

K

K

Y

F

K

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D

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L

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I

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N

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W

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binding heme c: C10 (= C32), C13 (= C35), H14 (= H36), P23 (= P45), Y25 (= Y47), L42 (= L64), R45 (≠ K67), Q51 (≠ K73), V53 (= V75), W54 (= W76), G55 (= G77), I57 (≠ V79), M59 (= M81), N62 (= N84)

2exvA Crystal structure of the f7a mutant of the cytochrome c551 from pseudomonas aeruginosa (see paper)
61% identity, 77% coverage: 24:102/102 of query aligns to 4:82/82 of 2exvA

query
sites
2exvA

E

E

A

V

L

L

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A

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K

A

N

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C
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C

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K

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G

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P

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Y

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K

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D

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A

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binding heme c: C12 (= C32), C15 (= C35), H16 (= H36), G24 (= G44), P25 (= P45), R47 (≠ K67), I48 (= I68), S52 (≠ G72), Q53 (≠ K73), G54 (= G74), V55 (= V75), W56 (= W76), G57 (= G77), I59 (≠ V79), P60 (= P80), M61 (= M81), N64 (= N84)

5xecA Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
62% identity, 77% coverage: 24:102/102 of query aligns to 4:82/82 of 5xecA

query
sites
5xecA

E

E

A

V

L

L

A

F

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K

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N

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C

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binding heme c: C12 (= C32), C15 (= C35), H16 (= H36), G24 (= G44), P25 (= P45), Y27 (= Y47), Y34 (= Y54), Y43 (≠ K63), I48 (= I68), S53 (≠ K73), G54 (= G74), V55 (= V75), W56 (= W76), G57 (= G77), V59 (= V79), M61 (= M81), Q64 (≠ N84), I78 (≠ V98)

1corA Investigation of the solution conformation of cytochromE C-551 from pseudomonas stutzeri (see paper)
59% identity, 80% coverage: 21:102/102 of query aligns to 1:82/82 of 1corA

query
sites
1corA

Q

Q

A

D

D

G

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A

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binding heme c: K10 (= K30), C12 (= C32), C15 (= C35), H16 (= H36), V23 (≠ L43), G24 (= G44), P25 (= P45), L44 (= L64), H47 (≠ K67), I48 (= I68), S52 (≠ G72), Q53 (≠ K73), G54 (= G74), V55 (= V75), W56 (= W76), G57 (= G77), I59 (≠ V79), M61 (= M81), N64 (= N84)

351cA Structure of cytochrome c551 from p. Aeruginosa refined at 1.6 angstroms resolution and comparison of the two redox forms (see paper)
59% identity, 77% coverage: 24:102/102 of query aligns to 4:82/82 of 351cA

query
sites
351cA

E

E

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K

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binding protoporphyrin ix containing fe: C12 (= C32), C15 (= C35), H16 (= H36), G24 (= G44), P25 (= P45), Y27 (= Y47), L44 (= L64), R47 (≠ K67), I48 (= I68), Q53 (≠ K73), G54 (= G74), V55 (= V75), W56 (= W76), G57 (= G77), I59 (≠ V79), P60 (= P80), M61 (= M81)

5aurA Hydrogenobacter thermophilus cytochrome c552 dimer formed by domain swapping at n-terminal region (see paper)
61% identity, 78% coverage: 23:102/102 of query aligns to 1:83/83 of 5aurA

query
sites
5aurA

D

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E

E

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L

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D

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binding heme c: C10 (= C32), C13 (= C35), H14 (= H36), P26 (= P45), Y28 (= Y47), Y35 (= Y54), Y44 (≠ K63), I49 (= I68), S54 (≠ K73), G55 (= G74), V56 (= V75), W57 (= W76), G58 (= G77), V60 (= V79), P61 (= P80), M62 (= M81), Q65 (≠ N84)

6kq1A Crystal structure of cytochrome c551 from pseudomonas sp. Strain mt-1.
60% identity, 80% coverage: 21:102/102 of query aligns to 1:82/82 of 6kq1A

query
sites
6kq1A

Q

Q

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D

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E

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A

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C
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C

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C

H
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H

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A

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G

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P

A

A

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A

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D

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L
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L

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H

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G
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W
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W

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M

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P

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N

N

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E
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E

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A

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binding heme c: C12 (= C32), C15 (= C35), H16 (= H36), G24 (= G44), P25 (= P45), V30 (= V50), Y34 (= Y54), L44 (= L64), H47 (≠ K67), I48 (= I68), Q53 (≠ K73), G54 (= G74), N55 (≠ V75), W56 (= W76), G57 (= G77), I59 (≠ V79), M61 (= M81), N64 (= N84)
binding zinc ion: D2 (≠ A22), E4 (= E24), K10 (= K30), E70 (= E90)

1cchA The solution conformation of cytochromE C-551 from p.Stutzeri zobell determined by nmr+ (see paper)
61% identity, 80% coverage: 21:102/102 of query aligns to 1:82/82 of 1cchA

query
sites
1cchA

Q

Q

A

D

D

G

E

E

A

A

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C

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A

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H

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K

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G

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A

Y

L

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K

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E

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A

A

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K

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N

K

A

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G

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E

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G

A

A

V

A

D

D

K

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L
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L

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A

A

L

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I
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I

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N

G

G

G
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G

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V

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W

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G

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M

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P

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P

N
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N

N

P

V

V

T

T

A

E

D

E

E

E

A

A

K

K

K

I

L

L

A

A

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E

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V

L

L

S

S

L

L

K

K

binding heme c: C12 (= C32), C15 (= C35), H16 (= H36), L44 (= L64), H47 (≠ K67), I48 (= I68), S52 (≠ G72), Q53 (≠ K73), V55 (= V75), W56 (= W76), G57 (= G77), I59 (≠ V79), M61 (= M81), P62 (= P82), N64 (= N84)

1a56A Primary sequence and solution conformation of ferricytochromE C-552 from nitrosomonas europaea, nmr, mean structure refined with explicit hydrogen bond constraints (see paper)
58% identity, 78% coverage: 23:102/102 of query aligns to 1:81/81 of 1a56A

query
sites
1a56A

D

D

E

A

A

D

L

L

A

A

K

K

A

K

K

N

N
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N

C
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C

M

I

S

A

C
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C

H
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H

Q

Q

V

V

A

E

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T

K

K

V

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V

G
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G

P
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P

A

A

Y

L

K

K

D

D

V

I

A

A

K

K

Y

Y

K

A

G

D

D

K

A

D

G

D

A

A

V

A

D

T

K

Y

L

L

A

A

A

G

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K

I
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I

K

K

A

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G

G

G
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S

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S

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G

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W

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G

E
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M
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M

P

P

N
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N

-
x
V

N
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N

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S

A

D

D

A

E

D

A

A

K

K

K

A

L

L

A

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T

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W

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L

L

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K

binding heme c: N9 (= N31), C10 (= C32), C13 (= C35), H14 (= H36), V21 (≠ L43), G22 (= G44), P23 (= P45), K45 (= K67), I46 (= I68), S50 (≠ G72), W54 (= W76), Q56 (≠ E78), I57 (≠ V79), M59 (= M81), N62 (= N84), V63 (vs. gap), N64 (= N85)

2d0sA Crystal structure of the cytochrome c552 from moderate thermophilic bacterium, hydrogenophilus thermoluteolus (see paper)
56% identity, 78% coverage: 23:102/102 of query aligns to 1:79/79 of 2d0sA

query
sites
2d0sA

D

D

E

E

A

A

L

L

A

A

K

K

A

A

K

K

N

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C

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C

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H

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I

A

D

V

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K

K

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P

A

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Y

Y

K

K

D

D

V

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A

A

A

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K

K

Y
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Y

K

-

G

-

D

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G

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D

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K

K

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L

A

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A

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K

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V

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K

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G

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W

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G

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M

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H

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-

A

E

D

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E

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K

K

binding heme c: C10 (= C32), C13 (= C35), H14 (= H36), P23 (= P45), Y32 (= Y54), K43 (= K67), V44 (≠ I68), A49 (≠ K73), G50 (= G74), V51 (= V75), W52 (= W76), G53 (= G77), V55 (= V79), M57 (= M81), P58 (= P82), H60 (≠ N84)

4xxlA Crystal structure of class 1 cytochrome mtod from sideroxydans lithotrophicus es-1 (see paper)
38% identity, 81% coverage: 19:101/102 of query aligns to 3:90/92 of 4xxlA

query
sites
4xxlA

N

D

A

V

Q

D

A

A

D

A

E

K

A

S

L

L

A

A

K

R

A

E

K

N

N

N

C
|
C

M

F

S

K

C
|
C

H
|
H

Q

G

V

V

A

D

V

K

K

E

V

K

L

D

G
|
G

P
|
P

A

S

Y
|
Y

K

K

D

K

V

V

A

A

A

E

K
|
K

Y
|
Y

K

R

G

G

D

K

A

A

G

D

A

A

V

E

D

A

K

K

L

L

A

I

A

H

K
x
H

I

V

K

T

A

S

G

G

G

E

K

K

G
x
A

V
x
K

W

F

-

P

-

D

-

G

-

H

G

E

E

E

V

E

P
x
H

M

K

P

N

P

I

N

N

N

G

V

K

T

A

A

S

D

P

E

E

A

A

-

I

K

K

K

N

L

L

A

V

T

D

W

W

V

I

L

L

S

S

L

L

binding heme c: C16 (= C32), C19 (= C35), H20 (= H36), G28 (= G44), P29 (= P45), Y31 (= Y47), K37 (= K53), Y38 (= Y54), H51 (≠ K67), A58 (≠ G74), K59 (≠ V75), H68 (≠ P80)

Query Sequence

>Dsui_2078 FitnessBrowser__PS:Dsui_2078
MKSTHLSLLALGGLFLLGNAQADEALAKAKNCMSCHQVAVKVLGPAYKDVAAKYKGDAGA
VDKLAAKIKAGGKGVWGEVPMPPNNVTADEAKKLATWVLSLK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory