SitesBLAST
Comparing Dsui_2542 FitnessBrowser__PS:Dsui_2542 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
52% identity, 100% coverage: 3:468/468 of query aligns to 5:465/465 of 3pm9A
- active site: A149 (= A149), L159 (≠ N159)
- binding flavin-adenine dinucleotide: P69 (= P67), Q70 (= Q68), G71 (= G69), G72 (= G70), N73 (= N71), T74 (= T72), G75 (≠ S73), L76 (= L74), G79 (≠ A77), Q80 (≠ A78), L91 (= L91), L133 (= L133), G134 (≠ A134), A135 (≠ S135), C139 (= C139), T140 (≠ Q140), G142 (= G142), G143 (= G143), S146 (= S146), T147 (= T147), A149 (= A149), G150 (= G150), E200 (= E200), G201 (= G201), I205 (= I205), I206 (= I206), E423 (= E426)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
38% identity, 99% coverage: 5:468/468 of query aligns to 11:464/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R330), T337 (≠ S334), K348 (= K345), Y379 (≠ F378), H381 (= H380), H388 (= H387), H423 (= H427)
- binding flavin-adenine dinucleotide: W39 (= W31), P75 (= P67), Q76 (= Q68), G77 (= G69), G78 (= G70), N79 (= N71), T80 (= T72), G81 (≠ S73), M82 (≠ L74), G85 (≠ A77), S86 (≠ A78), L139 (= L133), G140 (≠ A134), A141 (≠ S135), C145 (= C139), G149 (= G143), N150 (= N144), A152 (≠ S146), T153 (= T147), G157 (= G151), G207 (= G201), I212 (= I206), E422 (= E426), N459 (= N463)
- binding zinc ion: H381 (= H380), H388 (= H387), E422 (= E426)
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
38% identity, 99% coverage: 5:468/468 of query aligns to 11:464/466 of 6lpuA
- binding flavin-adenine dinucleotide: W39 (= W31), P75 (= P67), G77 (= G69), G78 (= G70), N79 (= N71), T80 (= T72), G81 (≠ S73), G85 (≠ A77), S86 (≠ A78), L139 (= L133), G140 (≠ A134), A141 (≠ S135), C145 (= C139), H146 (≠ Q140), G148 (= G142), G149 (= G143), N150 (= N144), A152 (≠ S146), T153 (= T147), A155 (= A149), E206 (= E200), G207 (= G201), I211 (= I205), I212 (= I206), E422 (= E426), N459 (= N463)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R330), T337 (≠ S334), K348 (= K345), Y379 (≠ F378), H381 (= H380), H388 (= H387), H423 (= H427)
- binding zinc ion: H381 (= H380), H388 (= H387), E422 (= E426)
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
38% identity, 99% coverage: 5:468/468 of query aligns to 11:464/466 of 6lpqA
- binding flavin-adenine dinucleotide: W39 (= W31), P75 (= P67), G77 (= G69), G78 (= G70), N79 (= N71), T80 (= T72), G81 (≠ S73), G85 (≠ A77), S86 (≠ A78), L139 (= L133), G140 (≠ A134), A141 (≠ S135), C145 (= C139), H146 (≠ Q140), G149 (= G143), N150 (= N144), A152 (≠ S146), T153 (= T147), A155 (= A149), G157 (= G151), E206 (= E200), G207 (= G201), I211 (= I205), I212 (= I206), E422 (= E426), N459 (= N463)
- binding d-malate: M82 (≠ L74), R333 (= R330), T337 (≠ S334), K348 (= K345), Y379 (≠ F378), H381 (= H380), H388 (= H387), E422 (= E426), H423 (= H427)
- binding zinc ion: H381 (= H380), H388 (= H387), E422 (= E426)
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
38% identity, 99% coverage: 5:468/468 of query aligns to 11:464/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R330), T337 (≠ S334), K348 (= K345), Y379 (≠ F378), H381 (= H380), H388 (= H387), N390 (= N389), H423 (= H427)
- binding flavin-adenine dinucleotide: W39 (= W31), P75 (= P67), G77 (= G69), G78 (= G70), N79 (= N71), T80 (= T72), G81 (≠ S73), M82 (≠ L74), G85 (≠ A77), S86 (≠ A78), L139 (= L133), G140 (≠ A134), A141 (≠ S135), C145 (= C139), G149 (= G143), N150 (= N144), A152 (≠ S146), T153 (= T147), A155 (= A149), G157 (= G151), G207 (= G201), I212 (= I206), E422 (= E426), H423 (= H427)
- binding zinc ion: H381 (= H380), H388 (= H387), E422 (= E426)
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
38% identity, 99% coverage: 5:468/468 of query aligns to 12:465/467 of 6lpnB
- binding flavin-adenine dinucleotide: W40 (= W31), P76 (= P67), G78 (= G69), G79 (= G70), N80 (= N71), T81 (= T72), G82 (≠ S73), M83 (≠ L74), G86 (≠ A77), S87 (≠ A78), L140 (= L133), A142 (≠ S135), C146 (= C139), H147 (≠ Q140), G150 (= G143), N151 (= N144), A153 (≠ S146), T154 (= T147), G208 (= G201), I212 (= I205), I213 (= I206), E423 (= E426), N460 (= N463)
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
38% identity, 99% coverage: 5:468/468 of query aligns to 64:517/521 of Q8N465
- S109 (≠ T48) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ V66) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G70) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ L88) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (≠ L94) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (= C113) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (= P130) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (≠ S146) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ P172) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G174) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ N319) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R330) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
- T390 (≠ S334) binding ; binding ; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ S343) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (= K345) binding ; binding ; mutation to A: Loss of catalytic activity.
- R419 (≠ L366) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ V373) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H380) binding ; mutation to A: Loss of catalytic activity.
- G436 (= G382) to V: slight reduction in catalytic activity
- N439 (= N385) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H387) binding ; mutation to A: Loss of catalytic activity.
- N443 (= N389) binding ; mutation to A: Significantly reduced catalytic activity.
- V444 (≠ L390) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (= A393) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E426) binding ; mutation to A: Loss of catalytic activity.
- H476 (= H427) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
- G477 (= G428) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
32% identity, 98% coverage: 10:468/468 of query aligns to 37:496/496 of P39976
Sites not aligning to the query:
- 17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
32% identity, 94% coverage: 30:467/468 of query aligns to 36:463/467 of 7qh2C
- binding flavin-adenine dinucleotide: V73 (≠ P67), G75 (= G69), S76 (≠ G70), G77 (≠ N71), T78 (= T72), G79 (≠ S73), L80 (= L74), A83 (= A77), C84 (≠ A78), P137 (≠ A134), G138 (≠ S135), E139 (= E136), A142 (≠ C139), T143 (≠ Q140), G146 (= G143), N147 (= N144), S149 (= S146), T150 (= T147), A152 (= A149), G153 (= G150), E203 (= E200), G204 (= G201), I209 (= I206), E422 (= E426), H423 (= H427)
- binding fe (iii) ion: H377 (= H380), H384 (= H387), E422 (= E426)
P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
33% identity, 100% coverage: 1:468/468 of query aligns to 1:456/459 of P9WIT1
- K354 (≠ E357) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
32% identity, 100% coverage: 3:468/468 of query aligns to 4:454/454 of 8jdvA
- binding 2-Ketohexanoic acid: V75 (≠ L74), R317 (= R330), W321 (vs. gap), H368 (= H380), H375 (= H387), H413 (= H427)
- binding flavin-adenine dinucleotide: P68 (= P67), G70 (= G69), T71 (≠ G70), G72 (≠ N71), T73 (= T72), G74 (≠ S73), G78 (≠ A77), V79 (≠ A78), L90 (= L91), P132 (≠ L133), G133 (≠ A134), A134 (≠ S135), G140 (= G143), M141 (≠ N144), A143 (≠ S146), T144 (= T147), A146 (= A149), S147 (≠ G150), E200 (= E200), G201 (= G201), I206 (= I206), W321 (vs. gap), Y322 (vs. gap), E412 (= E426), H413 (= H427), N449 (= N463)
- binding manganese (ii) ion: H368 (= H380), H375 (= H387), E412 (= E426)
8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
32% identity, 100% coverage: 3:468/468 of query aligns to 4:455/455 of 8jdeA
- binding flavin-adenine dinucleotide: P68 (= P67), G70 (= G69), T71 (≠ G70), G72 (≠ N71), T73 (= T72), G74 (≠ S73), G78 (≠ A77), V79 (≠ A78), L90 (= L91), P132 (≠ L133), G133 (≠ A134), A134 (≠ S135), G140 (= G143), M141 (≠ N144), A143 (≠ S146), T144 (= T147), A146 (= A149), S147 (≠ G150), E200 (= E200), G201 (= G201), I206 (= I206), W322 (vs. gap), E413 (= E426), H414 (= H427), N450 (= N463)
- binding lactic acid: R318 (= R330), H369 (= H380), H376 (= H387), H414 (= H427)
- binding manganese (ii) ion: H369 (= H380), H376 (= H387), E413 (= E426)
8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
32% identity, 100% coverage: 3:468/468 of query aligns to 4:456/456 of 8jdsA
- binding flavin-adenine dinucleotide: E32 (≠ W31), P68 (= P67), G70 (= G69), T71 (≠ G70), G72 (≠ N71), T73 (= T72), G74 (≠ S73), G78 (≠ A77), V79 (≠ A78), L90 (= L91), P132 (≠ L133), G133 (≠ A134), A134 (≠ S135), G140 (= G143), M141 (≠ N144), A143 (≠ S146), T144 (= T147), A146 (= A149), S147 (≠ G150), E200 (= E200), G201 (= G201), I206 (= I206), W323 (vs. gap), E414 (= E426), H415 (= H427), N451 (= N463)
- binding manganese (ii) ion: H370 (= H380), H377 (= H387), E414 (= E426)
- binding pyruvic acid: R319 (= R330), H370 (= H380), H377 (= H387), H415 (= H427)
8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
32% identity, 100% coverage: 3:468/468 of query aligns to 4:454/454 of 8jdzA
- binding (3S)-3-methyl-2-oxopentanoic acid: R318 (= R330), W322 (vs. gap), H369 (= H380), H376 (= H387), H413 (= H427)
- binding flavin-adenine dinucleotide: E32 (≠ W31), P68 (= P67), G70 (= G69), T71 (≠ G70), G72 (≠ N71), T73 (= T72), G74 (≠ S73), G78 (≠ A77), V79 (≠ A78), L90 (= L91), P132 (≠ L133), G133 (≠ A134), A134 (≠ S135), G140 (= G143), M141 (≠ N144), A143 (≠ S146), T144 (= T147), A146 (= A149), S147 (≠ G150), E200 (= E200), G201 (= G201), I206 (= I206), W322 (vs. gap), E412 (= E426), H413 (= H427), N449 (= N463)
- binding manganese (ii) ion: H369 (= H380), H376 (= H387), E412 (= E426)
8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
32% identity, 100% coverage: 3:468/468 of query aligns to 4:454/454 of 8jdyA
- binding 2-oxo-4-methylpentanoic acid: R318 (= R330), W322 (vs. gap), S336 (≠ K345), H369 (= H380), H376 (= H387), H413 (= H427)
- binding flavin-adenine dinucleotide: P68 (= P67), G70 (= G69), T71 (≠ G70), G72 (≠ N71), T73 (= T72), G74 (≠ S73), G78 (≠ A77), V79 (≠ A78), L90 (= L91), P132 (≠ L133), G133 (≠ A134), A134 (≠ S135), G140 (= G143), M141 (≠ N144), A143 (≠ S146), T144 (= T147), A146 (= A149), S147 (≠ G150), E200 (= E200), G201 (= G201), I206 (= I206), E412 (= E426), N449 (= N463)
- binding manganese (ii) ion: H369 (= H380), H376 (= H387), E412 (= E426)
8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
32% identity, 100% coverage: 3:468/468 of query aligns to 4:455/455 of 8jduA
- binding 2-oxopentanoic acid: R318 (= R330), W322 (vs. gap), H369 (= H380), H376 (= H387), H414 (= H427)
- binding flavin-adenine dinucleotide: P68 (= P67), G70 (= G69), T71 (≠ G70), G72 (≠ N71), T73 (= T72), G74 (≠ S73), G78 (≠ A77), V79 (≠ A78), L90 (= L91), P132 (≠ L133), G133 (≠ A134), A134 (≠ S135), G140 (= G143), M141 (≠ N144), A143 (≠ S146), T144 (= T147), A146 (= A149), S147 (≠ G150), E200 (= E200), G201 (= G201), I206 (= I206), W322 (vs. gap), E413 (= E426), N450 (= N463)
- binding manganese (ii) ion: H369 (= H380), H376 (= H387), E413 (= E426)
8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
32% identity, 100% coverage: 3:468/468 of query aligns to 4:455/455 of 8jdtA
- binding 2-ketobutyric acid: R318 (= R330), H369 (= H380), H376 (= H387), H414 (= H427)
- binding flavin-adenine dinucleotide: P68 (= P67), G70 (= G69), T71 (≠ G70), G72 (≠ N71), T73 (= T72), G74 (≠ S73), G78 (≠ A77), V79 (≠ A78), L90 (= L91), P132 (≠ L133), G133 (≠ A134), A134 (≠ S135), G140 (= G143), M141 (≠ N144), A143 (≠ S146), T144 (= T147), A146 (= A149), S147 (≠ G150), E200 (= E200), G201 (= G201), I206 (= I206), W322 (vs. gap), E413 (= E426), H414 (= H427), N450 (= N463)
- binding manganese (ii) ion: H369 (= H380), H376 (= H387), E413 (= E426)
8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
32% identity, 100% coverage: 3:468/468 of query aligns to 4:455/455 of 8jdxA
- binding flavin-adenine dinucleotide: E32 (≠ W31), P68 (= P67), G70 (= G69), T71 (≠ G70), G72 (≠ N71), T73 (= T72), G74 (≠ S73), G78 (≠ A77), V79 (≠ A78), L90 (= L91), P132 (≠ L133), G133 (≠ A134), A134 (≠ S135), G140 (= G143), M141 (≠ N144), A143 (≠ S146), T144 (= T147), A146 (= A149), S147 (≠ G150), E200 (= E200), G201 (= G201), I206 (= I206), W322 (vs. gap), E413 (= E426), H414 (= H427), N450 (= N463)
- binding 3-methyl-2-oxobutanoic acid: R318 (= R330), H369 (= H380), H376 (= H387), H414 (= H427)
- binding manganese (ii) ion: H369 (= H380), H376 (= H387), E413 (= E426)
8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
32% identity, 100% coverage: 3:468/468 of query aligns to 4:455/455 of 8jdpA
- binding flavin-adenine dinucleotide: P68 (= P67), G70 (= G69), T71 (≠ G70), G72 (≠ N71), T73 (= T72), G74 (≠ S73), G78 (≠ A77), V79 (≠ A78), L90 (= L91), P132 (≠ L133), G133 (≠ A134), A134 (≠ S135), G140 (= G143), M141 (≠ N144), A143 (≠ S146), T144 (= T147), A146 (= A149), S147 (≠ G150), E200 (= E200), G201 (= G201), I206 (= I206), H369 (= H380), E413 (= E426), N450 (= N463)
- binding deaminohydroxyvaline: R319 (= R330), H414 (= H427)
8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
31% identity, 100% coverage: 3:468/468 of query aligns to 4:456/456 of 8jdrA
- binding flavin-adenine dinucleotide: P68 (= P67), G70 (= G69), T71 (≠ G70), G72 (≠ N71), T73 (= T72), G74 (≠ S73), G78 (≠ A77), V79 (≠ A78), L90 (= L91), P132 (≠ L133), G133 (≠ A134), A134 (≠ S135), G140 (= G143), M141 (≠ N144), A143 (≠ S146), T144 (= T147), A146 (= A149), S147 (≠ G150), E200 (= E200), G201 (= G201), I206 (= I206), Y324 (vs. gap), H370 (= H380), E414 (= E426), N451 (= N463)
- binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: R319 (= R330), W323 (vs. gap), H415 (= H427)
Query Sequence
>Dsui_2542 FitnessBrowser__PS:Dsui_2542
MNDLQQQLAAIVGPVHVLTGEAELAPYLTDWRGRYRGRALALVKPGSTDEVAAVVRACAA
AGVPMVPQGGNTSLCGAATPDQGGCSVLVNLSRLNRIRQIDAANNAITVEAGCLLAQVQE
AAAAAGRLFPLALASEGSCQIGGNLSTNAGGVQVLRYGNTRELTLGLEVVLPDGRLWNGL
TALRKDNTGYDLKDLFIGAEGTLGIITAAVLKLFPRPRAVVTAWLAVADGAAAIALLGRA
QARFDARLTAFELISRQSLDLVLQHIPGSRQPLAAPAPWQVLLELADGGAWADLQADLED
FIGGEMADGRVQDGVLAQNETQARQLWALRENISEAQKIEGLSIKHDISVPVSRIPEFLD
LAGTALAAAFPGVRVVAFGHAGDGNLHYNLSKAQRQDNDTFIAATPQANRIVHDLVARLG
GSISAEHGIGQLKREELVRYKDPVGLDLMRCIKSSLDPRGLMNPGKIL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory