SitesBLAST
Comparing Dsui_3045 FitnessBrowser__PS:Dsui_3045 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8x9vC Crystal structure of xanthomonas oryzae pv. Oryzae nadh-quinone oxidoreductase subunits nuoe and nuof
58% identity, 91% coverage: 18:419/442 of query aligns to 20:410/416 of 8x9vC
- binding flavin mononucleotide: G61 (= G59), G63 (= G61), K72 (= K70), N88 (= N87), D90 (= D89), S92 (≠ G91), G180 (= G178), E181 (= E179), E182 (= E180), N216 (= N214), N217 (= N215), T220 (= T218)
7arcF Cryo-em structure of polytomella complex-i (peripheral arm) (see paper)
53% identity, 90% coverage: 23:421/442 of query aligns to 27:426/430 of 7arcF
- binding flavin mononucleotide: G61 (= G59), R62 (= R60), K72 (= K70), N90 (= N87), D92 (= D89), E93 (= E90), S94 (≠ G91), Y178 (= Y175), G181 (= G178), E182 (= E179), T217 (≠ N214), N218 (= N215), L401 (= L396)
- binding iron/sulfur cluster: S352 (= S347), C353 (= C348), G354 (= G349), Q355 (= Q350), C356 (= C351), C359 (= C354), T397 (= T392), C399 (= C394), L401 (= L396)
8qbyF Respiratory complex i from paracoccus denitrificans in msp2n2 nanodiscs
51% identity, 91% coverage: 22:422/442 of query aligns to 19:420/421 of 8qbyF
- binding flavin mononucleotide: G54 (= G59), R55 (= R60), G56 (= G61), K65 (= K70), N83 (= N87), E86 (= E90), Y171 (= Y175), G174 (= G178), E175 (= E179), V209 (≠ I213), N210 (= N214), N211 (= N215), S214 (≠ T218)
- binding iron/sulfur cluster: I172 (= I176), P190 (= P194), C346 (= C348), G347 (= G349), Q348 (= Q350), C349 (= C351), C352 (= C354), T390 (= T392), C392 (= C394), L394 (= L396), G395 (= G397)
P49821 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial; NDUFV1; Complex I-51kD; CI-51kD; NADH dehydrogenase flavoprotein 1; NADH-ubiquinone oxidoreductase 51 kDa subunit; EC 7.1.1.2 from Homo sapiens (Human) (see paper)
52% identity, 92% coverage: 17:421/442 of query aligns to 47:452/464 of P49821
- C379 (= C348) binding [4Fe-4S] cluster
- C382 (= C351) binding [4Fe-4S] cluster
- C385 (= C354) binding [4Fe-4S] cluster
- C425 (= C394) binding [4Fe-4S] cluster
8b9zF Drosophila melanogaster complex i in the active state (dm1) (see paper)
50% identity, 91% coverage: 17:420/442 of query aligns to 29:433/441 of 8b9zF
- binding flavin mononucleotide: G69 (= G59), G71 (= G61), K80 (= K70), N98 (= N87), D100 (= D89), G189 (= G178), E191 (= E180), N226 (= N215), A408 (= A395), L409 (= L396)
- binding iron/sulfur cluster: P205 (= P194), C361 (= C348), G362 (= G349), Q363 (= Q350), C364 (= C351), C367 (= C354), T405 (= T392), C407 (= C394), L409 (= L396)
7dgq8 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (see paper)
52% identity, 92% coverage: 17:421/442 of query aligns to 16:421/427 of 7dgq8
- binding flavin mononucleotide: G58 (= G61), K67 (= K70), N85 (= N87), D87 (= D89), E88 (= E90), G89 (= G91), C175 (= C177), G176 (= G178), A212 (≠ N214), N213 (= N215)
- binding iron/sulfur cluster: S347 (= S347), C348 (= C348), G349 (= G349), C351 (= C351), C354 (= C354), C394 (= C394), L396 (= L396)
- binding : R121 (≠ T123), Y132 (≠ W134), Q139 (≠ E141), R143 (≠ D145), Y146 (≠ R148), E147 (≠ A149), F165 (= F167), R168 (≠ H170)
6zk91 Peripheral domain of open complex i during turnover (see paper)
52% identity, 92% coverage: 17:421/442 of query aligns to 19:424/430 of 6zk91
- binding flavin mononucleotide: G59 (= G59), G61 (= G61), K70 (= K70), N88 (= N87), D90 (= D89), E91 (= E90), G179 (= G178), E180 (= E179), A215 (≠ N214), N216 (= N215), A398 (= A395), L399 (= L396)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G61 (= G61), G62 (= G62), A63 (= A63), F65 (= F65), K70 (= K70), E93 (= E92), Y176 (= Y175), E181 (= E180), F201 (= F200), T323 (≠ S320)
- binding iron/sulfur cluster: I177 (= I176), P195 (= P194), C351 (= C348), G352 (= G349), Q353 (= Q350), C354 (= C351), C357 (= C354), T395 (= T392), C397 (= C394), L399 (= L396)
8eswV1 NADH dehydrogenase (Ubiquinone) 24 kDa subunit, isoform A (see paper)
50% identity, 91% coverage: 17:420/442 of query aligns to 27:431/439 of 8eswV1
- binding flavin mononucleotide: G67 (= G59), G69 (= G61), K78 (= K70), N96 (= N87), D98 (= D89), E99 (= E90), G187 (= G178), E188 (= E179), N224 (= N215), A406 (= A395)
- binding iron/sulfur cluster: I185 (= I176), P203 (= P194), C359 (= C348), Q361 (= Q350), C362 (= C351), C365 (= C354), T403 (= T392), I404 (= I393), C405 (= C394), L407 (= L396)
- binding : Y143 (≠ W134), N144 (≠ E135), Q150 (≠ E141), D174 (≠ E165)
5lnk1 Entire ovine respiratory complex i (see paper)
52% identity, 92% coverage: 17:421/442 of query aligns to 21:426/432 of 5lnk1
- binding fe2/s2 (inorganic) cluster: P96 (= P93), G97 (= G94)
- binding flavin mononucleotide: G61 (= G59), R62 (= R60), K72 (= K70), N90 (= N87), D92 (= D89), E93 (= E90), G94 (= G91), Y178 (= Y175), G181 (= G178), E182 (= E179), V216 (≠ I213), A217 (≠ N214), N218 (= N215), T221 (= T218), L401 (= L396)
- binding iron/sulfur cluster: P197 (= P194), S352 (= S347), C353 (= C348), Q355 (= Q350), C356 (= C351), C359 (= C354), T397 (= T392), I398 (= I393), C399 (= C394)
8gymv1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (see paper)
52% identity, 90% coverage: 23:420/442 of query aligns to 24:423/442 of 8gymv1
- binding flavin mononucleotide: G58 (= G59), G60 (= G61), N88 (= N87), D90 (= D89), Y176 (= Y175), E180 (= E179), E181 (= E180), T215 (≠ N214), N216 (= N215), T219 (= T218), A398 (= A395), L399 (= L396)
- binding iron/sulfur cluster: I177 (= I176), P195 (= P194), C351 (= C348), G352 (= G349), Q353 (= Q350), C354 (= C351), C357 (= C354), T395 (= T392), C397 (= C394), L399 (= L396), G400 (= G397)
7v2cA Active state complex i from q10 dataset (see paper)
52% identity, 92% coverage: 17:421/442 of query aligns to 22:427/433 of 7v2cA
- binding flavin mononucleotide: G62 (= G59), K73 (= K70), N91 (= N87), Y179 (= Y175), G182 (= G178), E183 (= E179), N219 (= N215), A401 (= A395), L402 (= L396)
- binding iron/sulfur cluster: P198 (= P194), C354 (= C348), G355 (= G349), Q356 (= Q350), C357 (= C351), C360 (= C354), T398 (= T392), C400 (= C394), L402 (= L396)
P25708 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial; NDUFV1; Complex I-51kD; CI-51kD; NADH dehydrogenase flavoprotein 1; NADH-ubiquinone oxidoreductase 51 kDa subunit; EC 7.1.1.2 from Bos taurus (Bovine) (see paper)
52% identity, 90% coverage: 17:415/442 of query aligns to 47:446/464 of P25708
- C379 (= C348) binding [4Fe-4S] cluster
- C382 (= C351) binding [4Fe-4S] cluster
- C385 (= C354) binding [4Fe-4S] cluster
- C425 (= C394) binding [4Fe-4S] cluster
7aqrF Cryo-em structure of arabidopsis thaliana complex-i (peripheral arm) (see paper)
52% identity, 88% coverage: 23:411/442 of query aligns to 26:415/434 of 7aqrF
- binding flavin mononucleotide: G60 (= G59), R61 (= R60), G62 (= G61), K71 (= K70), N89 (= N87), D91 (= D89), Y177 (= Y175), G180 (= G178), E181 (= E179), E182 (= E180), T216 (≠ N214), N217 (= N215)
- binding iron/sulfur cluster: S351 (= S347), C352 (= C348), G353 (= G349), Q354 (= Q350), C355 (= C351), C358 (= C354), T396 (= T392), C398 (= C394), L400 (= L396)
Q91YT0 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial; NDUFV1; Complex I-51kD; CI-51kD; NADH-ubiquinone oxidoreductase 51 kDa subunit; EC 7.1.1.2 from Mus musculus (Mouse) (see 2 papers)
51% identity, 92% coverage: 17:421/442 of query aligns to 47:452/464 of Q91YT0
- C379 (= C348) binding [4Fe-4S] cluster
- C382 (= C351) binding [4Fe-4S] cluster
- C385 (= C354) binding [4Fe-4S] cluster
- C425 (= C394) binding [4Fe-4S] cluster
Sites not aligning to the query:
- 1:20 modified: transit peptide, Mitochondrion
4hea1 Crystal structure of the entire respiratory complex i from thermus thermophilus (see paper)
49% identity, 94% coverage: 3:416/442 of query aligns to 4:421/437 of 4hea1
- binding flavin mononucleotide: G63 (= G59), K74 (= K70), N91 (= N87), D93 (= D89), Y179 (= Y175), G182 (= G178), E183 (= E179), N218 (= N214), N219 (= N215), L401 (= L396)
- binding iron/sulfur cluster: I180 (= I176), P198 (= P194), S351 (= S347), C352 (= C348), G353 (= G349), K354 (≠ Q350), C355 (= C351), C358 (= C354), F398 (≠ I393), C399 (= C394), L401 (= L396)
2ybb1 Fitted model for bovine mitochondrial supercomplex i1iii2iv1 by single particle cryo-em (emd-1876) (see paper)
49% identity, 94% coverage: 3:416/442 of query aligns to 4:421/437 of 2ybb1
- binding flavin mononucleotide: G63 (= G59), G65 (= G61), N91 (= N87), D93 (= D89), G182 (= G178), E183 (= E179), E184 (= E180), N218 (= N214), N219 (= N215), T222 (= T218), P400 (≠ A395)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G65 (= G61), G66 (= G62), F69 (= F65), K74 (= K70), F77 (= F73), E96 (= E92), Y179 (= Y175), E184 (= E180), K201 (= K197), F204 (= F200), T324 (≠ S320)
- binding iron/sulfur cluster: S351 (= S347), C352 (= C348), K354 (≠ Q350), C355 (= C351), C358 (= C354), F398 (≠ I393), C399 (= C394), L401 (= L396), A402 (≠ G397)
Q56222 NADH-quinone oxidoreductase subunit 1; NADH dehydrogenase I chain 1; NDH-1 subunit 1; EC 7.1.1.- from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
49% identity, 94% coverage: 3:416/442 of query aligns to 5:422/438 of Q56222
- K75 (= K70) binding FMN
- NADES 92:96 (≠ NSDEG 87:91) binding FMN
- YICGEE 180:185 (= YICGEE 175:180) binding FMN
- INN 218:220 (= INN 213:215) binding FMN
- C353 (= C348) binding [4Fe-4S] cluster
- C356 (= C351) binding [4Fe-4S] cluster
- C359 (= C354) binding [4Fe-4S] cluster
- C400 (= C394) binding [4Fe-4S] cluster
7zm7B Cryoem structure of mitochondrial complex i from chaetomium thermophilum (inhibited by ddm) (see paper)
50% identity, 89% coverage: 22:415/442 of query aligns to 24:421/456 of 7zm7B
- binding flavin mononucleotide: G59 (= G59), G61 (= G61), K70 (= K70), N91 (= N87), D93 (= D89), G182 (= G178), E183 (= E179), E184 (= E180), A218 (≠ N214), N219 (= N215), A401 (= A395), L402 (= L396)
- binding iron/sulfur cluster: P198 (= P194), C354 (= C348), G355 (= G349), Q356 (= Q350), C357 (= C351), C360 (= C354), T398 (= T392), C400 (= C394), L402 (= L396)
8e9hF Mycobacterial respiratory complex i, fully-inserted quinone (see paper)
49% identity, 94% coverage: 7:420/442 of query aligns to 3:422/436 of 8e9hF
- binding flavin mononucleotide: G53 (= G59), R54 (= R60), G55 (= G61), A57 (= A63), K64 (= K70), N90 (= N87), D92 (= D89), Y178 (= Y175), G181 (= G178), E182 (= E179), E183 (= E180), N217 (= N214), N218 (= N215), S221 (≠ T218), L398 (= L396)
- binding iron/sulfur cluster: P197 (= P194), S349 (= S347), C350 (= C348), G351 (= G349), K352 (≠ Q350), C353 (= C351), C356 (= C354), S394 (≠ T392), F395 (≠ I393), C396 (= C394), L398 (= L396), G399 (= G397)
- binding zinc ion: C333 (= C331), E371 (= E369), H420 (= H418)
Sites not aligning to the query:
8j9iV1 ndufv1 (see paper)
50% identity, 92% coverage: 18:423/442 of query aligns to 30:438/504 of 8j9iV1
- binding flavin mononucleotide: G69 (= G59), G71 (= G61), K80 (= K70), N100 (= N87), D102 (= D89), E103 (= E90), G191 (= G178), E192 (= E179), V226 (≠ I213), N228 (= N215), T231 (= T218), A410 (= A395), L411 (= L396)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G71 (= G61), G72 (= G62), F75 (= F65), K80 (= K70), F83 (= F73), E105 (= E92), Y188 (= Y175), E193 (= E180), F213 (= F200), T335 (≠ S320), A410 (= A395)
- binding iron/sulfur cluster: P207 (= P194), S362 (= S347), C363 (= C348), N364 (≠ G349), Q365 (= Q350), C366 (= C351), C369 (= C354), C409 (= C394), L411 (= L396)
Query Sequence
>Dsui_3045 FitnessBrowser__PS:Dsui_3045
MAILGAINPTLTAGWNGDAGWSLKDYVARGGYEALKKILAEKIPQEQVIAEVKTSVLRGR
GGAGFPTGLKWSFMPRSFPGDKYVVCNSDEGEPGTFKDRDILRYNPHSVIEGMAIAAYAM
GATRGYNYIHGEIWEVYKRFEDALDEARAAGFIGQNILGSGFNFELFAHHGYGAYICGEE
TALLESIEGKKGQPRFKPPFPASFGLYGKPTTINNTETFGSIPFIIRDGGEKFLNLGKPN
NGGTKLFSISGHVNRPGNYEIPLGTPFSTLLEMAGGVRGGRKLKAVIPGGSSAPVLPGDV
MMECTMDYDSISKAGSMLGSGAVIVMDETTCMVKALERLSFFYHEESCGQCTPCREGTPW
LYKVVSRIEHGLGRPDDLDLLDSVCEGIMGRTICALGDAAAFPVKSFIKHFRDEFVHHIE
HKTCLVPKDVQRAGSGFFKAQN
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory