SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Dsui_3064 Dsui_3064 carbamoyl-phosphate synthase, large subunit to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1bxrA Structure of carbamoyl phosphate synthetase complexed with the atp analog amppnp (see paper)
70% identity, 100% coverage: 1:1067/1068 of query aligns to 1:1072/1073 of 1bxrA

query
sites
1bxrA

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active site: R129 (= R129), R169 (= R169), M174 (≠ L174), G176 (= G176), K202 (= K202), E215 (= E215), H243 (= H243), N283 (= N283), Q285 (= Q285), E299 (= E299), N301 (= N301), R303 (= R303), S307 (= S307), D338 (≠ E338), G507 (≠ A502), K634 (= K628), R715 (= R709), G721 (= G715), G722 (= G716), S745 (= S739), E761 (= E755), D769 (= D763), Q829 (= Q823), E841 (= E835), N843 (= N837), R848 (= R842), P901 (= P895)
binding phosphoaminophosphonic acid-adenylate ester: R129 (= R129), I167 (= I167), R169 (= R169), T173 (= T173), M174 (≠ L174), G175 (= G175), G176 (= G176), E208 (= E208), S209 (= S209), L210 (= L210), I211 (= I211), E215 (= E215), M240 (= M240), G241 (= G241), I242 (≠ V242), H243 (= H243), T244 (= T244), Q285 (= Q285), I298 (= I298), E299 (= E299), N301 (= N301), R306 (= R306), T376 (= T376), R675 (= R669), V713 (= V707), R715 (= R709), V719 (= V713), L720 (= L714), G721 (= G715), G722 (= G716), M725 (= M719), D753 (= D747), H754 (≠ R748), F755 (= F749), L756 (= L750), E761 (= E755), A785 (= A779), G786 (= G780), V787 (= V781), H788 (= H782), S789 (= S783), Q829 (= Q823), I840 (≠ L834), E841 (= E835), N843 (= N837), R848 (= R842), P909 (= P903)
binding manganese (ii) ion: E299 (= E299), N301 (= N301), Q829 (= Q823), E841 (= E835), E841 (= E835), N843 (= N837)
binding L-ornithine: E783 (= E777), D791 (= D785), A793 (= A787), E892 (= E886), V893 (≠ A887), L907 (= L901), H1039 (≠ T1034), Y1040 (≠ M1035), D1041 (≠ Y1036), T1042 (= T1037)

P00968 Carbamoyl-phosphate synthase large chain; Carbamoyl-phosphate synthetase ammonia chain; EC 6.3.5.5 from Escherichia coli (strain K12) (see 6 papers)
70% identity, 100% coverage: 1:1067/1068 of query aligns to 1:1072/1073 of P00968

query
sites
P00968

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M1 (= M1) modified: Initiator methionine, Removed
Q285 (= Q285) binding
E299 (= E299) binding ; binding
N301 (= N301) binding
Q829 (= Q823) binding
E841 (= E835) binding ; binding
N843 (= N837) binding

1t36A Crystal structure of e. Coli carbamoyl phosphate synthetase small subunit mutant c248d complexed with uridine 5'-monophosphate (see paper)
69% identity, 100% coverage: 1:1067/1068 of query aligns to 1:1057/1058 of 1t36A

query
sites
1t36A

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F

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Y

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x
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T

T

T

I

L

W

N

G

G

A

G

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F

A

A

A

T

A

A

A

M

G

A

I

L

R

N

Q

A

G

D

G

A

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T

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E

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A

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I

I

active site: R129 (= R129), R169 (= R169), M174 (≠ L174), G176 (= G176), K202 (= K202), E215 (= E215), H243 (= H243), N283 (= N283), Q285 (= Q285), E299 (= E299), N301 (= N301), R303 (= R303), S307 (= S307), D338 (≠ E338), G507 (≠ A502), K634 (= K628), R715 (= R709), E746 (= E755), D754 (= D763), Q814 (= Q823), E826 (= E835), N828 (= N837), R833 (= R842), P886 (= P895)
binding adenosine-5'-diphosphate: R129 (= R129), I167 (= I167), R169 (= R169), T173 (= T173), M174 (≠ L174), G175 (= G175), G176 (= G176), E208 (= E208), S209 (= S209), L210 (= L210), I211 (= I211), E215 (= E215), M240 (= M240), G241 (= G241), I242 (≠ V242), H243 (= H243), T244 (= T244), Q285 (= Q285), I298 (= I298), E299 (= E299), T376 (= T376), P690 (= P684), V713 (= V707), R715 (= R709), M718 (= M719), H739 (≠ R748), F740 (= F749), L741 (= L750), E746 (= E755), A770 (= A779), G771 (= G780), V772 (= V781), H773 (= H782), S774 (= S783), Q814 (= Q823), I825 (≠ L834), E826 (= E835), P894 (= P903)
binding manganese (ii) ion: Q285 (= Q285), E299 (= E299), E299 (= E299), N301 (= N301), Q814 (= Q823), E826 (= E835)
binding L-ornithine: E768 (= E777), D776 (= D785), S777 (= S786), A778 (= A787), E877 (= E886), V878 (≠ A887), L892 (= L901), Y1025 (≠ M1035), D1026 (≠ Y1036), T1027 (= T1037)
binding phosphate ion: M174 (≠ L174), G175 (= G175), H243 (= H243), Q285 (= Q285), E299 (= E299), N301 (= N301), R303 (= R303), R306 (= R306)
binding uridine-5'-monophosphate: S933 (= S942), V934 (= V943), R935 (≠ K944), K939 (= K948), T959 (= T968), H960 (≠ R969), G961 (= G970), T962 (= T971), K978 (= K987), N1000 (= N1009), T1001 (= T1010), T1002 (≠ V1011), D1010 (= D1020), S1011 (= S1021), I1014 (= I1024)

1c3oA Crystal structure of the carbamoyl phosphate synthetase: small subunit mutant c269s with bound glutamine (see paper)
69% identity, 100% coverage: 1:1067/1068 of query aligns to 1:1057/1058 of 1c3oA

query
sites
1c3oA

M

M

P

P

K

K

R

R

T

T

D

D

I

I

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K

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I

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I

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L

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A

A

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I

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I

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I

I

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G

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A

A

C

C

E

E

F

F

D

D

Y

Y

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S

G

G

A

A

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Q

A

A

C

C

K

K

A

A

L

L

K

R

A

E

E

E

G

G

Y

Y

R

R

V

V

I

I

L

L

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V

N

N

S

S

N

N

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P

A

A

T

T

I

I

M

M

T

T

D

D

P

P

E

E

T

M

A

A

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E

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K

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I

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A

L

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L

L

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M

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G

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T

A

A

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L

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A

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M

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G

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G

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Y

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M

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