SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_0145 FitnessBrowser__Cola:Echvi_0145 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7bxsA 2-amino-3-ketobutyrate coa ligase from cupriavidus necator glycine binding form
65% identity, 99% coverage: 3:397/397 of query aligns to 5:399/399 of 7bxsA

query
sites
7bxsA

E

E

S

A

L

F

K

Y

P

A

K

S

L

I

E

R

K

T

E

E

L

L

K

E

E

S

I

I

S

R

D

A

A

A

G

G

L

L

Y

F

K

K

S

N

E

E

R

R

I

V

I

I

T

A

S

T

P

P

Q

Q

G

G

A

A

E

R

I

V

S

R

T

T

S

T

E

D

G

G

K

R

Q

E

V

V

L

I

N

N

F

L

C

C

A

A

N
|
N

N

N

Y

Y

L

L

G

G

L

L

S

S

S

S

H

H

P

P

K

Q

V

V

I

I

E

E

A

A

A

A

K

H

N

E

A

A

I

L

D

R

T

T

H

H

G

G

Y

F

G

G

M

L

S

S

S

S

V
|
V

R

R

F

F

I

I

C

C

G

G

T

T

Q

Q

D

D

I

L

H

H

K

K

E

T

L

L

E

E

R

A

K

R

I

L

S

S

E

A

F

F

L

L

G

G

T

T

E

E

D

D

T

T

I

I

L

L

Y

Y

A

G

A
x
S

A
|
A

F
|
F

D

D

A

A

N
|
N

G

G

G

G

V

L

F

F

E

E

P

T

I

L

L

L

G

G

P

A

E

E

D

D

A

A

I

V

I

I

S

S

D

D

A

A

L

L

N

N

H
|
H

A

A

S
|
S

I

I

I

I

D

D

G

G

V

V

R

R

L

L

C

S

K

K

A

A

M

R

R

R

F

Y

R

R

Y

Y

K

Q

H

H

N

N

D

D

M

M

E

D

D

D

L

L

E

R

T

V

Q

Q

L

L

K

E

E

Q

A

A

N

R

A

A

K

D

G

G

A

A

K

R

Q

Y

K

T

I

L

I

V

V

F

T

S

D
|
D

G

G

A

V

F

F

S
|
S

M

M

D

D

G

G

T

T

I

V

A

A

Q

R

M

L

D

D

K

E

I

M

V

R

A

A

L

I

A

C

E

D

Q

E

Y

Y

D

G

A

A

L

L

V

L

M

G

S

I

D
|
D

E

E

C
|
C

H
|
H

S

A

T

T

G

G

F

F

I

M

G

G

K

Q

T

R

G

G

R

R

G

G

V

T

H

H

E

E

L

A

K

R

G

G

V

V

M

F

G

G

K

K

M

I

D

D

I

I

I

I

T

T

G

G

T
|
T

L

L

G

G

K

A

A

A

L

L

G

G

G

G

A

A

S

S

G

G

G

G

F

F

T

T

S

S

G

A

R

R

K

K

E

E

I

V

I

V

D

A

I

L

L

L

R

R

Q

Q

R

R

S

S

R

R

P

P

Y

Y

L

L

F
|
F

S
|
S

N
|
N

T

T

L

V

A

A

P

P

A

A

I

I

T

V

G

G

A

A

S

S

I

I

A

A

V

V

F

L

D

D

L

I

L

L

S

E

E

A

T

S

T

T

E

E

L

L

R

R

D

D

K

R

L

L

E

E

D

G

N

N

T

T

T

R

Y

F

F

F

R

R

E

A

K

G

M

L

T

D

A

R

A

L

G

G

F

F

D

D

I

V

K

K

P

A

G

G

V

D

H

H

P

P

I

I

V

I

P

P

I

I

M

M

L

V

Y

Y

D

D

A

A

V

D

L

K

S

A

Q

Q

Q

Q

M

L

A

A

E

Q

K

R

L

L

L

L

E

E

R

L

G

G

V

V

Y

Y

V

V

I

V

G

G

F

F

Y

F

Y

Y

P

P

V

V

V

V

P

P

K

K

G

G

Q

Q

A

A

R

R

I

I

R
|
R

V

V

Q

Q

I

M

S

S

A

A

A

L

H

H

D

D

R

E

K

A

H

A

L

L

D

Q

A

A

A

A

I

L

E

D

A

A

F

F

T

G

E

Q

V

A

G

G

K

R

E

E

L

L

G

G

V

L

I

I

binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: N52 (= N50), V81 (= V79), S112 (≠ A110), A113 (= A111), F114 (= F112), N117 (= N115), H138 (= H136), S140 (= S138), D183 (= D181), S187 (= S185), D212 (= D210), C214 (= C212), H215 (= H213), T243 (= T241), F275 (= F273), S276 (= S274), N277 (= N275), R370 (= R368)

7bxrA 2-amino-3-ketobutyrate coa ligase from cupriavidus necator 3- hydroxynorvaline binding form
65% identity, 99% coverage: 3:397/397 of query aligns to 5:399/399 of 7bxrA

query
sites
7bxrA

E

E

S

A

L

F

K

Y

P

A

K

S

L

I

E

R

K

T

E

E

L

L

K

E

E

S

I

I

S

R

D

A

A

A

G

G

L

L

Y

F

K

K

S

N

E

E

R

R

I

V

I

I

T

A

S

T

P

P

Q

Q

G

G

A

A

E

R

I

V

S

R

T

T

S

T

E

D

G

G

K

R

Q

E

V

V

L

I

N

N

F

L

C

C

A

A

N
|
N

N

N

Y

Y

L

L

G

G

L

L

S

S

S

S

H

H

P

P

K

Q

V

V

I

I

E

E

A

A

A

A

K

H

N

E

A

A

I

L

D

R

T

T

H

H

G

G

Y

F

G

G

M

L

S

S

S

S

V

V

R

R

F
|
F

I

I

C

C

G

G

T

T

Q

Q

D

D

I

L

H

H

K

K

E

T

L

L

E

E

R

A

K

R

I

L

S

S

E

A

F

F

L

L

G

G

T

T

E

E

D

D

T

T

I

I

L

L

Y

Y

A

G

A
x
S

A
|
A

F
|
F

D

D

A

A

N
|
N

G

G

G

G

V

L

F

F

E

E

P

T

I

L

L

L

G

G

P

A

E

E

D

D

A

A

I

V

I

I

S

S

D

D

A

A

L

L

N

N

H
|
H

A

A

S
|
S

I

I

I

I

D

D

G

G

V

V

R

R

L

L

C

S

K

K

A

A

M

R

R

R

F

Y

R

R

Y

Y

K

Q

H

H

N

N

D

D

M

M

E

D

D

D

L

L

E

R

T

V

Q

Q

L

L

K

E

E

Q

A

A

N

R

A

A

K

D

G

G

A

A

K

R

Q

Y

K

T

I

L

I

V

V

F

T

S

D
|
D

G

G

A

V

F

F

S
|
S

M
|
M

D

D

G

G

T

T

I

V

A

A

Q

R

M

L

D

D

K

E

I

M

V

R

A

A

L

I

A

C

E

D

Q

E

Y

Y

D

G

A

A

L

L

V

L

M

G

S

I

D
|
D

E

E

C
|
C

H
|
H

S

A

T

T

G

G

F

F

I

M

G

G

K

Q

T

R

G

G

R

R

G

G

V

T

H

H

E

E

L

A

K

R

G

G

V

V

M

F

G

G

K

K

M

I

D

D

I

I

I

I

T

T

G

G

T
|
T

L

L

G

G

K

A

A

A

L

L

G

G

G

G

A

A

S

S

G

G

G

G

F

F

T

T

S

S

G

A

R

R

K

K

E

E

I

V

I

V

D

A

I

L

L

L

R

R

Q

Q

R

R

S

S

R

R

P

P

Y

Y

L
|
L

F
|
F

S
|
S

N
|
N

T

T

L

V

A

A

P

P

A

A

I

I

T

V

G

G

A

A

S

S

I

I

A

A

V

V

F

L

D

D

L

I

L

L

S

E

E

A

T

S

T

T

E

E

L

L

R

R

D

D

K

R

L

L

E

E

D

G

N

N

T

T

T

R

Y

F

F

F

R

R

E

A

K

G

M

L

T

D

A

R

A

L

G

G

F

F

D

D

I

V

K

K

P

A

G

G

V

D

H

H

P

P

I

I

V

I

P

P

I

I

M

M

L

V

Y

Y

D

D

A

A

V

D

L

K

S

A

Q

Q

Q

Q

M

L

A

A

E

Q

K

R

L

L

L

L

E

E

R

L

G

G

V

V

Y

Y

V

V

I

V

G

G

F

F

Y

F

Y

Y

P

P

V

V

V

V

P

P

K

K

G

G

Q

Q

A

A

R

R

I

I

R
|
R

V

V

Q

Q

I

M

S

S

A

A

A

L

H

H

D

D

R

E

K

A

H

A

L

L

D

Q

A

A

A

A

I

L

E

D

A

A

F

F

T

G

E

Q

V

A

G

G

K

R

E

E

L

L

G

G

V

L

I

I

binding (2S,3R)-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]-3-oxidanyl-pentanoic acid: N52 (= N50), F83 (= F81), S112 (≠ A110), A113 (= A111), F114 (= F112), N117 (= N115), H138 (= H136), S140 (= S138), D183 (= D181), S187 (= S185), M188 (= M186), D212 (= D210), C214 (= C212), H215 (= H213), T243 (= T241), L274 (= L272), F275 (= F273), S276 (= S274), N277 (= N275), R370 (= R368)

7bxqA 2-amino-3-ketobutyrate coa ligase from cupriavidus necator l-threonine binding form
65% identity, 99% coverage: 3:397/397 of query aligns to 5:399/399 of 7bxqA

query
sites
7bxqA

E

E

S

A

L

F

K

Y

P

A

K

S

L

I

E

R

K

T

E

E

L

L

K

E

E

S

I

I

S

R

D

A

A

A

G

G

L

L

Y

F

K

K

S

N

E

E

R

R

I

V

I

I

T

A

S

T

P

P

Q

Q

G

G

A

A

E

R

I

V

S

R

T

T

S

T

E

D

G

G

K

R

Q

E

V

V

L

I

N

N

F

L

C

C

A

A

N
|
N

N

N

Y

Y

L

L

G

G

L

L

S

S

S

S

H

H

P

P

K

Q

V

V

I

I

E

E

A

A

A

A

K

H

N

E

A

A

I

L

D

R

T

T

H

H

G

G

Y

F

G

G

M

L

S

S

S

S

V
|
V

R

R

F

F

I

I

C

C

G

G

T

T

Q

Q

D

D

I

L

H

H

K

K

E

T

L

L

E

E

R

A

K

R

I

L

S

S

E

A

F

F

L

L

G

G

T

T

E

E

D

D

T

T

I

I

L

L

Y

Y

A

G

A
x
S

A
|
A

F
|
F

D

D

A

A

N
|
N

G

G

G

G

V

L

F

F

E

E

P

T

I

L

L

L

G

G

P

A

E

E

D

D

A

A

I

V

I

I

S

S

D

D

A

A

L

L

N

N

H
|
H

A

A

S
|
S

I

I

I

I

D

D

G

G

V

V

R

R

L

L

C

S

K

K

A

A

M

R

R

R

F

Y

R

R

Y

Y

K

Q

H

H

N

N

D

D

M

M

E

D

D

D

L

L

E

R

T

V

Q

Q

L

L

K

E

E

Q

A

A

N

R

A

A

K

D

G

G

A

A

K

R

Q

Y

K

T

I

L

I

V

V

F

T

S

D
|
D

G

G

A

V

F

F

S
|
S

M
|
M

D

D

G

G

T

T

I

V

A

A

Q

R

M

L

D

D

K

E

I

M

V

R

A

A

L

I

A

C

E

D

Q

E

Y

Y

D

G

A

A

L

L

V

L

M

G

S

I

D
|
D

E

E

C
|
C

H
|
H

S

A

T

T

G

G

F

F

I

M

G

G

K

Q

T

R

G

G

R

R

G

G

V

T

H

H

E

E

L

A

K

R

G

G

V

V

M

F

G

G

K

K

M

I

D

D

I

I

I

I

T

T

G

G

T
|
T

L

L

G

G

K

A

A

A

L

L

G

G

G

G

A

A

S

S

G

G

G

G

F

F

T

T

S

S

G

A

R

R

K

K

E

E

I

V

I

V

D

A

I

L

L

L

R

R

Q

Q

R

R

S

S

R

R

P

P

Y

Y

L
|
L

F
|
F

S
|
S

N
|
N

T

T

L

V

A

A

P

P

A

A

I

I

T

V

G

G

A

A

S

S

I

I

A

A

V

V

F

L

D

D

L

I

L

L

S

E

E

A

T

S

T

T

E

E

L

L

R

R

D

D

K

R

L

L

E

E

D

G

N

N

T

T

T

R

Y

F

F

F

R

R

E

A

K

G

M

L

T

D

A

R

A

L

G

G

F

F

D

D

I

V

K

K

P

A

G

G

V

D

H

H

P

P

I

I

V

I

P

P

I

I

M

M

L

V

Y

Y

D

D

A

A

V

D

L

K

S

A

Q

Q

Q

Q

M

L

A

A

E

Q

K

R

L

L

L

L

E

E

R

L

G

G

V

V

Y

Y

V

V

I

V

G

G

F

F

Y

F

Y

Y

P

P

V

V

V

V

P

P

K

K

G

G

Q

Q

A

A

R

R

I

I

R
|
R

V

V

Q

Q

I

M

S

S

A

A

A

L

H

H

D

D

R

E

K

A

H

A

L

L

D

Q

A

A

A

A

I

L

E

D

A

A

F

F

T

G

E

Q

V

A

G

G

K

R

E

E

L

L

G

G

V

L

I

I

binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-allothreonine: N52 (= N50), V81 (= V79), S112 (≠ A110), A113 (= A111), F114 (= F112), N117 (= N115), H138 (= H136), S140 (= S138), D183 (= D181), S187 (= S185), M188 (= M186), D212 (= D210), C214 (= C212), H215 (= H213), T243 (= T241), L274 (= L272), F275 (= F273), S276 (= S274), N277 (= N275), R370 (= R368)

3tqxA Structure of the 2-amino-3-ketobutyrate coenzyme a ligase (kbl) from coxiella burnetii (see paper)
64% identity, 98% coverage: 8:397/397 of query aligns to 7:396/396 of 3tqxA

query
sites
3tqxA

K

Q

L

L

E

N

K

K

E

E

L

I

K

E

E

G

I

L

S

K

D

K

A

A

G

G

L

L

Y

Y

K

K

S

S

E

E

R

R

I

I

I

I

T

T

S

S

P

P

Q

Q

G

N

A

A

E

E

I

I

S

K

T

V

S

G

E

E

G

-

K

K

Q

E

V

V

L

L

N

N

F

F

C

C

A

A

N
|
N

N

N

Y

Y

L

L

G

G

L

L

S

A

S

D

H

H

P

P

K

A

V

L

I

I

E

K

A

T

A

A

K

Q

N

T

A

V

I

V

D

E

T

Q

H

Y

G

G

Y

F

G

G

M

M

S

A

S

S

V

V

R

R

F

F

I

I

C

C

G

G

T

T

Q

Q

D

T

I

I

H

H

K

K

E

E

L

L

E

E

R

K

K

D

I

I

S

S

E

E

F

F

L

L

G

G

T

T

E

D

D

D

T

T

I

I

L

L

Y

Y

A

S

A
x
S

A
x
C

F
|
F

D

D

A

A

N
|
N

G

G

G

G

V

L

F

F

E

E

P

T

I

L

L

L

G

G

P

P

E

E

D

D

A

A

I

I

I

I

S

S

D

D

A

E

L

L

N

N

H
|
H

A

A

S
|
S

I

I

I

I

D

D

G

G

V

I

R

R

L

L

C

C

K

K

A

A

M

Q

R

R

F

Y

R

R

Y

Y

K

K

H

N

N

N

D

A

M

M

E

G

D

D

L

L

E

E

T

A

Q

K

L

L

K

K

E

E

A

A

N

D

A

E

K

K

G

G

A

A

K

R

Q

F

K

K

I

L

I

I

V

A

T

T

D
|
D

G

G

A

V

F

F

S
|
S

M

M

D

D

G

G

T

I

I

I

A

A

Q

D

M

L

D

K

K

S

I

I

V

C

A

D

L

L

A

A

E

D

Q

K

Y

Y

D

N

A

A

L

L

V

V

M

M

S

V

D
|
D

E

D

C
x
S

H
|
H

S

A

T

V

G

G

F

F

I

I

G

G

K

E

T

N

G

G

R

R

G

G

V

T

H

P

E

E

L

Y

K

C

G

G

V

V

M

A

G

D

K

R

M

V

D

D

I

I

I

L

T

T

G

G

T
|
T

L

L

G

G

K
|
K

A

A

L

L

G

G

G

G

A

A

S

S

G

G

G

G

F

Y

T

T

S

S

G

G

R

H

K

K

E

E

I

I

I

I

D

E

I

W

L

L

R

R

Q

N

R

R

S

S

R

R

P

P

Y

Y

L

L

F
|
F

S
|
S

N
|
N

T

T

L

V

A

A

P

P

A

V

I

I

T

V

G

A

A

T

S

S

I

L

A

K

V

V

F

L

D

E

L

L

L

L

-

K

S

T

E

E

T

G

T

P

E

Q

L

L

R

R

D

K

K

Q

L

L

E

Q

D

E

N

N

T

S

T

R

Y

Y

F

F

R

R

E

A

K

G

M

M

T

E

A

K

A

L

G

G

F

F

D

Q

I

L

K

V

P

P

G

G

V

N

H

H

P

P

I

I

V

I

P

P

I

V

M

M

L

L

Y

G

D

D

A

A

V

Q

L

L

S

A

Q

T

Q

N

M

M

A

A

E

D

K

H

L

L

L

L

E

Q

R

E

G

G

V

I

Y

Y

V

V

I

V

G

G

F

F

Y

S

Y

Y

P

P

V

V

V

V

P

P

K

M

G

G

Q

K

A

A

R

R

I

I

R

R

V

V

Q

Q

I

M

S

S

A

A

A

V

H

H

D

T

R

Q

K

Q

H

Q

L

L

D

D

A

R

A

A

I

I

E

E

A

A

F

F

T

G

E

Q

V

V

G

G

K

K

E

K

L

L

G

G

V

A

I

I

active site: N48 (= N50), H134 (= H136), D179 (= D181), S183 (= S185), D208 (= D210), H211 (= H213), K242 (= K244)
binding pyridoxal-5'-phosphate: S108 (≠ A110), C109 (≠ A111), F110 (= F112), N113 (= N115), H134 (= H136), S136 (= S138), D208 (= D210), S210 (≠ C212), H211 (= H213), T239 (= T241), K242 (= K244), F271 (= F273), S272 (= S274), N273 (= N275)

P0AB77 2-amino-3-ketobutyrate coenzyme A ligase; AKB ligase; Glycine acetyltransferase; EC 2.3.1.29 from Escherichia coli (strain K12) (see paper)
61% identity, 100% coverage: 1:397/397 of query aligns to 5:397/398 of P0AB77

query
sites
P0AB77

M

F

Y

Y

E

Q

S

Q

L

L

K

T

P

N

K

D

L

L

E

E

K

T

E

A

L

R

K

A

E

E

I

-

S

-

D

-

A

-

G

G

L

L

Y

F

K

K

S

E

E

E

R

R

I

I

I

I

T

T

S

S

P

A

Q

Q

G

Q

A

A

E

D

I

I

S

T

T

V

S

A

E

D

G

G

K

S

Q

H

V

V

L

I

N

N

F

F

C

C

A

A

N

N

N

N

Y

Y

L

L

G

G

L

L

S

A

S

N

H

H

P

P

K

D

V

L

I

I

E

A

A

A

A

A

K

K

N

A

A

G

I

M

D

D

T

S

H

H

G

G

Y

F

G

G

M

M

S

A

S

S

V

V

R

R

F

F

I

I

C

C

G

G

T

T

Q

Q

D

D

I

S

H

H

K

K

E

E

L

L

E

E

R

Q

K

K

I

L

S

A

E

A

F

F

L

L

G

G

T

M

E

E

D

D

T

A

I

I

L

L

Y

Y

A

S

A

S

A

C

F

F

D

D

A

A

N

N

G

G

G

G

V

L

F

F

E

E

P

T

I

L

L

L

G

G

P

A

E

E

D

D

A

A

I

I

I

I

S

S

D

D

A

A

L

L

N

N

H
|
H

A

A

S

S

I

I

I

I

D

D

G

G

V

V

R

R

L

L

C

C

K

K

A

A

M

K

R

R

F

Y

R

R

Y

Y

K

A

H

N

N

N

D

D

M

M

E

Q

D

E

L

L

E

E

T

A

Q

R

L

L

K

K

E

E

A

A

N

R

A

E

K

A

G

G

A

A

K

R

Q

H

K

V

I

L

I

I

V

A

T

T

D

D

G

G

A

V

F

F

S
|
S

M

M

D

D

G

G

T

V

I

I

A

A

Q

N

M

L

D

K

K

G

I

V

V

C

A

D

L

L

A

A

E

D

Q

K

Y

Y

D

D

A

A

L

L

V

V

M

M

S

V

D

D

E

D

C

S

H

H

S

A

T

V

G

G

F

F

I

V

G

G

K

E

T

N

G

G

R

R

G

G

V

S

H

H

E

E

L

Y

K

C

G

D

V

V

M

M

G

G

K

R

M

V

D

D

I

I

I

I

T

T

G

G

T

T

L

L

G

G

K

K

A

A

L

L

G

G

G

G

A

A

S

S

G

G

G

G

F

Y

T

T

S

A

G

A

R

R

K

K

E

E

I

V

I

V

D

E

I

W

L

L

R

R

Q

Q

R

R

S

S

R

R

P

P

Y

Y

L

L

F

F

S

S

N

N

T

S

L

L

A

A

P

P

A

A

I

I

T

V

G

A

A

A

S

S

I

I

A

K

V

V

F

L

D

E

L

M

L

V

S

E

E

A

T

G

T

S

E

E

L

L

R

R

D

D

K

R

L

L

E

W

D

A

N

N

T

A

T

R

Y

Q

F

F

R

R

E

E

K

Q

M

M

T

S

A

A

A

A

G

G

F

F

D

T

I

L

K

A

P

G

G

A

V

D

H

H

P

A

I

I

V

I

P

P

I

V

M

M

L

L

Y

G

D

D

A

A

V

V

L

V

S

A

Q

Q

Q

K

M

F

A

A

E

R

K

E

L

L

L

Q

E

K

R

E

G

G

V

I

Y

Y

V

V

I

T

G

G

F

F

Y

F

Y

Y

P

P

V

V

V

V

P

P

K

K

G

G

Q

Q

A

A

R

R

I

I

R
|
R

V

T

Q

Q

I

M

S

S

A

A

A

A

H

H

D

T

R

P

K

E

H

Q

L

I

D

T

A

R

A

A

I

V

E

E

A

A

F

F

T

T

E

R

V

I

G

G

K

K

E

Q

L

L

G

G

V

V

I

I

H136 (= H136) binding
S185 (= S185) binding in other chain
R368 (= R368) binding

1fc4A 2-amino-3-ketobutyrate coa ligase (see paper)
61% identity, 100% coverage: 1:397/397 of query aligns to 8:400/401 of 1fc4A

query
sites
1fc4A

M

F

Y

Y

E

Q

S

Q

L

L

K

T

P

N

K

D

L

L

E

E

K

T

E

A

L

R

K

A

E

E

I

-

S

-

D

-

A

-

G

G

L

L

Y

F

K

K

S

E

E

E

R

R

I

I

I

I

T

T

S

S

P

A

Q

Q

G

Q

A

A

E

D

I

I

S

T

T

V

S

A

E

D

G

G

K

S

Q

H

V

V

L

I

N

N

F

F

C

C

A

A

N
|
N

N

N

Y

Y

L

L

G

G

L

L

S

A

S

N

H

H

P

P

K

D

V

L

I

I

E

A

A

A

A

A

K

K

N

A

A

G

I

M

D

D

T

S

H

H

G

G

Y

F

G

G

M

M

S

A

S

S

V

V

R

R

F

F

I

I

C

C

G

G

T

T

Q

Q

D

D

I

S

H

H

K

K

E

E

L

L

E

E

R

Q

K

K

I

L

S

A

E

A

F

F

L

L

G

G

T

M

E

E

D

D

T

A

I

I

L

L

Y

Y

A

S

A
x
S

A
x
C

F
|
F

D

D

A

A

N

N

G

G

G

G

V

L

F

F

E

E

P

T

I

L

L

L

G

G

P

A

E

E

D

D

A

A

I

I

I

I

S

S

D

D

A

A

L

L

N

N

H
|
H

A

A

S

S

I

I

I

I

D

D

G

G

V

V

R

R

L

L

C

C

K

K

A

A

M

K

R

R

F

Y

R

R

Y

Y

K

A

H

N

N

N

D

D

M

M

E

Q

D

E

L

L

E

E

T

A

Q

R

L

L

K

K

E

E

A

A

N

R

A

E

K

A

G

G

A

A

K

R

Q

H

K

V

I

L

I

I

V

A

T

T

D
|
D

G

G

A

V

F

F

S
|
S

M
|
M

D

D

G

G

T

V

I

I

A

A

Q

N

M

L

D

K

K

G

I

V

V

C

A

D

L

L

A

A

E

D

Q

K

Y

Y

D

D

A

A

L

L

V

V

M

M

S

V

D
|
D

E

D

C
x
S

H
|
H

S

A

T

V

G

G

F

F

I

V

G

G

K

E

T

N

G

G

R

R

G

G

V

S

H

H

E

E

L

Y

K

C

G

D

V

V

M

M

G

G

K

R

M

V

D

D

I

I

I

I

T

T

G

G

T
|
T

L

L

G

G

K
|
K

A

A

L

L

G

G

G

G

A

A

S

S

G

G

G

G

F

Y

T

T

S

A

G

A

R

R

K

K

E

E

I

V

I

V

D

E

I

W

L

L

R

R

Q

Q

R

R

S

S

R

R

P

P

Y

Y

L

L

F
|
F

S
|
S

N
|
N

T

S

L

L

A

A

P

P

A

A

I

I

T

V

G

A

A

A

S

S

I

I

A

K

V

V

F

L

D

E

L

M

L

V

S

E

E

A

T

G

T

S

E

E

L

L

R

R

D

D

K

R

L

L

E

W

D

A

N

N

T

A

T

R

Y

Q

F

F

R

R

E

E

K

Q

M

M

T

S

A

A

A

A

G

G

F

F

D

T

I

L

K

A

P

G

G

A

V

D

H

H

P

A

I

I

V

I

P

P

I

V

M

M

L

L

Y

G

D

D

A

A

V

V

L

V

S

A

Q

Q

Q

K

M

F

A

A

E

R

K

E

L

L

L

Q

E

K

R

E

G

G

V

I

Y

Y

V

V

I

T

G

G

F

F

Y

F

Y

Y

P

P

V

V

V

V

P

P

K

K

G

G

Q

Q

A

A

R

R

I

I

R
|
R

V

T

Q

Q

I

M

S

S

A

A

A

A

H

H

D

T

R

P

K

E

H

Q

L

I

D

T

A

R

A

A

I

V

E

E

A

A

F

F

T

T

E

R

V

I

G

G

K

K

E

Q

L

L

G

G

V

V

I

I

active site: N53 (= N50), H139 (= H136), D184 (= D181), S188 (= S185), D213 (= D210), H216 (= H213), K247 (= K244)
binding 2-amino-3-ketobutyric acid: N53 (= N50), H139 (= H136), S188 (= S185), M189 (= M186), H216 (= H213), K247 (= K244), R371 (= R368)
binding pyridoxal-5'-phosphate: S113 (≠ A110), C114 (≠ A111), F115 (= F112), H139 (= H136), D184 (= D181), S188 (= S185), D213 (= D210), S215 (≠ C212), H216 (= H213), T244 (= T241), K247 (= K244), F276 (= F273), S277 (= S274), N278 (= N275)

7v58B Structural insights into the substrate selectivity of acyl-coa transferase (see paper)
65% identity, 95% coverage: 20:397/397 of query aligns to 22:399/400 of 7v58B

query
sites
7v58B

G

G

L

L

Y

Y

K

K

S

S

E

E

R

R

I

I

I

I

T

T

S

S

P

Q

Q

Q

G

Q

A

A

E

A

I

V

S

K

T

I

S

A

E

S

G

G

K

E

Q

E

V

V

L

L

N

N

F

F

C

C

A

A

N

N

N

N

Y

Y

L

L

G

G

L

L

S

A

S

N

H

H

P

P

K

A

V

L

I

I

E

E

A

A

A

G

K

K

N

A

A

G

I

M

D

D

T

E

H

H

G

G

Y

F

G

G

M

M

S

A

S

S

V

V

R

R

F

F

I

I

C

C

G

G

T

T

Q

Q

D

D

I

I

H

H

K

K

E

E

L

L

E

E

R

Q

K

K

I

L

S

S

E

T

F

F

L

L

G

G

T

K

E

E

D

D

T

T

I

I

L

L

Y

Y

A

T

A

S

A
x
C

F

F

D

D

A

A

N

N

G

A

G

G

V

L

F

F

E

E

P

T

I

I

L

L

G

D

P

K

E

E

D

D

A

A

I

I

I

I

S

S

D

D

A

A

L

L

N

N

H
|
H

A

A

S

S

I

I

I

I

D

D

G

G

V

V

R

R

L

L

C

C

K

K

A

A

M

M

R

R

F

F

R

R

Y

Y

K

S

H

N

N

N

D

N

M

M

E

T

D

E

L

L

E

E

T

E

Q

Q

L

L

K

I

E

A

A

A

N

K

A

D

K

A

G

G

A

A

K

R

Q

N

K

I

I

L

I

I

V

V

T

T

D

D

G

G

A

V

F

F

S

S

M

M

D

D

G

G

T

V

I

V

A

A

Q

N

M

L

D

P

K

A

I

I

V

C

A

D

L

L

A

A

E

E

Q

K

Y

Y

D

G

A

A

L

L

V

T

M

M

S

V

D
|
D

E

D

C
x
S

H
|
H

S

A

T

V

G

G

F

F

I

M

G

G

K

K

T

T

G

G

R

A

G

G

V

T

H

H

E

E

L

Y

K

H

G

D

V

V

M

V

G

D

K

R

M

I

D

D

I

I

I

I

T

T

G

G

T

T

L

L

G

G

K
|
K

A

A

L

M

G

G

G

G

A

A

S

S

G

G

G

G

F

Y

T

T

S

S

G

G

R

K

K

K

E

E

I

V

I

I

D

E

I

W

L

L

R

R

Q

Q

R

R

S

S

R

R

P

P

Y

Y

L

L

F
|
F

S
|
S

N
|
N

T

S

L

V

A

A

P

P

A

A

I

I

T

V

G

S

A

A

S

S

I

I

A

R

V

V

F

L

D

D

L

L

L

L

S

Q

E

E

T

S

T

G

E

D

L

L

R

R

D

D

K

R

L

L

E

W

D

E

N

N

T

A

T

T

Y

H

F

F

R

R

E

T

K

R

M

M

T

S

A

E

A

A

G

G

F

F

D

T

I

L

K

A

P

G

G

A

V

D

H

H

P

A

I

I

V

I

P

P

I

I

M

M

L

L

Y

G

D

D

A

A

V

K

L

V

S

A

Q

A

Q

E

M

F

A

A

E

E

K

R

L

A

L

L

E

E

R

K

G

G

V

I

Y

Y

V

V

I

I

G

G

F

F

Y

S

Y

F

P

P

V

V

V

V

P

P

K

K

G

G

Q

Q

A

A

R

R

I

I

R

R

V

T

Q

Q

I

M

S

S

A

A

A

A

H

H

D

S

R

R

K

E

H

Q

L

L

D

D

A

K

A

A

I

I

E

D

A

A

F

F

T

I

E

E

V

V

G

G

K

R

E

D

L

M

G

E

V

I

I

I

binding glycine: C113 (≠ A111), H138 (= H136), D212 (= D210), S214 (≠ C212), H215 (= H213), K246 (= K244)
binding pyridoxal-5'-phosphate: F275 (= F273), S276 (= S274), N277 (= N275)

Q0P5L8 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial; AKB ligase; Aminoacetone synthase; Glycine acetyltransferase; EC 2.3.1.29 from Bos taurus (Bovine) (see paper)
56% identity, 99% coverage: 5:397/397 of query aligns to 27:418/419 of Q0P5L8

query
sites
Q0P5L8

L

L

K

R

P

G

K

I

L

L

E

E

K

E

E

E

L

L

K

E

E

S

I

I

S

R

D

G

A

A

G

G

L

T

Y

W

K

K

S

S

E

E

R

R

I

V

I

I

T

T

S

S

P

R

Q

Q

G

G

A

P

E

H

I

I

S

H

T

V

S

D

E

G

G

A

K

P

Q

G

-

G

V

I

L

I

N

N

F

F

C

C

A

A

N

N

N

N

Y

Y

L

L

G

G

L

L

S

S

S

S

H

H

P

P

K

E

V

V

I

I

E

Q

A

A

A

G

K

L

N

R

A

T

I

L

D

K

T

E

H

F

G

G

Y

A

G

G

M

L

S

S

S

S

V

V

R

R

F

F

I

I

C

C

G

G

T

T

Q

Q

D

S

I

I

H

H

K

K

E

D

L

L

E

E

R

A

K

K

I

I

S

A

E

R

F

F

L

H

G

Q

T

R

E

E

D

D

T

A

I

I

L

L

Y

Y

A

P

A

S

A

C

F

F

D

D

A

A

N

N

G

T

G

G

V

L

F

F

E

E

P

A

I

L

L

L

G

T

P

S

E

E

D

D

A

A

I

V

I

L

S

S

D

D

A

E

L

L

N

N

H

H

A

A

S

S

I

I

I

I

D

D

G

G

V

I

R

R

L

L

C

C

K

K

A

A

M

H

R

K

F

Y

R

R

Y

Y

K

R

H

H

N

L

D

D

M

M

E

A

D

D

L

L

E

E

T

A

Q

K

L

L

K

Q

E

E

A

A

N

Q

A

K

K

H

G

-

A

-

K

R

Q

L

K

R

I

L

I

V

V

A

T

T

D

D

G

G

A

A

F

F

S

S

M

M

D

D

G

G

T

D

I

I

A

A

Q

P

M

L

D

Q

K

E

I

I

V

C

A

R

L

L

A

A

E

S

Q

Q

Y

Y

D

G

A

A

L

L

V

V

M

F

S

V

D

D

E

E

C

S

H

H

S

A

T

T

G

G

F

F

I

L

G

G

K

A

T

T

G

G

R

R

G

G

V

T

H

D

E

E

L

L

K

L

G

G

V

V

M

M

G

D

K

Q

M

V

D

T

I

I

I

I

T

N

G

S

T

T

L

L

G

G

K
|
K

A

A

L

L

G

G

G

G

A

A

S

S

G

G

G

G

F

Y

T

T

S

T

G

G

R

P

K

G

E

A

I

L

I

V

D

S

I

L

L

L

R

R

Q

Q

R

R

S

A

R

R

P

P

Y

Y

L

L

F

F

S

S

N

N

T

S

L

L

A

P

P

P

A

A

I

A

T

V

G

G

A

C

S

A

I

S

A

K

V

A

F

L

D

D

L

L

L

L

S

M

E

E

T

S

T

N

E

A

L

I

R

V

D

Q

K

S

L

M

E

A

D

A

N

K

T

T

T

L

Y

R

F

F

R

R

E

S

K

Q

M

M

T

E

A

A

A

A

G

G

F

F

D

T

I

I

K

S

P

G

G

A

V

N

H

H

P

P

I

I

V

C

P

P

I

V

M

M

L

L

Y

G

D

D

A

A

V

R

L

L

S

A

Q

L

Q

N

M

I

A

A

E

D

K

D

L

M

L

L

E

K

R

R

G

G

V

I

Y

F

V

V

I

I

G

G

F

F

Y

S

Y

Y

P

P

V

V

V

V

P

P

K

K

G

G

Q

K

A

A

R

R

I

I

R

R

V

V

Q

Q

I

I

S

S

A

A

A

V

H

H

D

S

R

E

K

E

H

D

L

I

D

D

A

R

A

C

I

V

E

E

A

A

F

F

T

V

E

E

V

V

G

G

K

R

E

L

L

H

G

G

V

A

I

L

K265 (= K244) modified: N6-(pyridoxal phosphate)lysine

Sites not aligning to the query:

1:21 modified: transit peptide, Mitochondrion

7poaA An irreversible, promiscuous and highly thermostable claisen- condensation biocatalyst drives the synthesis of substituted pyrroles
40% identity, 99% coverage: 5:397/397 of query aligns to 8:397/398 of 7poaA

query
sites
7poaA

L

L

K

R

P

A

K

R

L

V

E

R

K

E

E

E

L

L

K

E

E

R

I

L

S

K

D

R

A

E

G

G

L

L

Y

Y

K

I

S

S

E

P

R

K

I

V

I

L

T

E

S

A

P

P

Q

Q

G

-

A

E

E

P

I

V

S

T

T

R

S

V

E

E

G

G

K

R

Q

E

V

V

L

V

N

N

F

L

C

A

A

S

N

N

N

N

Y

Y

L

L

G

G

L

F

S

A

S

N

H

H

P

P

K

Y

V

L

I

K

E

E

A

K

A

A

K

R

N

Q

A

Y

I

L

D

E

T

K

H

W

G

G

Y

A

G

G

M

S

S

G

S

A

V

V

R

R

F

T

I

I

C

A

G

G

T

T

Q

F

D

T

I

Y

H

H

K

V

E

E

L

L

E

E

R

E

K

A

I

L

S

A

E

R

F

F

L

K

G

G

T

T

E

E

D

S

T

A

I

L

L

V

Y

L

A

Q

A
x
S

A
x
G

F
|
F

D

T

A

A

N
|
N

G

Q

G

G

V

V

F

L

E

G

P

A

I

L

L

L

G

K

P

E

E

G

D

D

A

V

I

V

I

F

S

S

D

D

A

E

L

L

N

N

H
|
H

A

A

S
|
S

I

I

I

I

D

D

G

G

V

L

R

R

L

L

C

T

K

K

A

A

M

T

R

R

F

L

R

V

Y

F

K

R

H

H

N

A

D

D

M

V

E

A

D

H

L

L

E

E

T

E

Q

L

L

L

K

K

E

A

A

H

N

D

A

T

K

D

G

G

A

L

K

K

Q

-

K

-

I

L

I

I

V

V

T

T

D
|
D

G

G

A

V

F

F

S

S

M

M

D

D

G

G

T

D

I

I

A

A

Q

P

M

L

D

D

K

K

I

I

V

V

A

P

L

L

A

A

E

K

Q

K

Y

Y

D

K

A

A

L

V

V

V

M

Y

S

V

D
|
D

E

D

C
x
A

H
|
H

S

G

T

S

G

G

F

V

I

L

G

G

K

E

T

K

G

G

R

K

G

G

V

T

H

V

E

H

L

H

K

F

G

G

V

F

M

H

G

Q

K

D

M

P

D

D

I

V

I

V

-

Q

T

V

G

A

T
|
T

L

L

G
x
S

K
|
K

A

A

L

W

G

A

G

G

A

I

S

G

G

G

G

Y

F

A

T

A

S

G

G

A

R

R

K

-

E

E

I

L

I

K

D

D

I

L

L

L

R

I

Q

N

R

K

S

A

R

R

P

P

Y

F

L

L

F
|
F

S
|
S

N
x
T

T

S

L

H

A

P

P

P

A

A

I

V

T

V

G

G

A

A

S

L

I

L

A

G

V

A

F

L

D

E

L

L

L

I

S

E

E

K

T

E

T

P

E

E

L

R

R

V

D

E

K

R

L

L

E

W

D

E

N

N

T

T

T

R

Y

Y

F

F

R

K

E

R

K

E

M

L

T

A

A

R

A

L

G

G

F

Y

D

D

I

T

K

L

P

G

G

S

V

Q

H

T

P

P

I

I

V

T

P

P

I

V

M

L

L

F

Y

G

D

E

A

A

V

P

L

L

S

A

Q

F

Q

E

M

A

A

S

E

R

K

L

L

L

L

L

E

E

R

E

G

G

V

V

Y

F

V

A

I

V

G

G

F

I

Y

G

Y

F

P

P

V

T

V

V

P

P

K

R

G

G

Q

K

A

A

R

R

I

I

R

R

V

N

Q

I

I

V

S

T

A

A

A

A

H

H

D

T

R

K

K

E

H

M

L

L

D

D

A

K

A

A

I

L

E

E

A

A

F

Y

T

E

E

K

V

V

G

G

K

K

E

R

L

L

G

G

V

I

I

I

binding pyridoxal-5'-phosphate: S112 (≠ A110), G113 (≠ A111), F114 (= F112), N117 (= N115), H138 (= H136), S140 (= S138), D181 (= D181), D210 (= D210), A212 (≠ C212), H213 (= H213), T242 (= T241), S244 (≠ G243), K245 (= K244), F273 (= F273), S274 (= S274), T275 (≠ N275)

8h29A Serine palmitoyltransferase (see paper)
36% identity, 96% coverage: 11:393/397 of query aligns to 13:391/394 of 8h29A

query
sites
8h29A

K

K

E

I

L

V

K

E

E

E

I

L

S

K

D

A

A

K

G

G

L

L

Y

Y

K

A

S

Y

E

F

R

R

I

P

I

I

T

Q

S

S

P

K

Q

Q

G

D

A

T

E

E

I

V

S

K

T

I

S

-

E

D

G

G

K

R

Q

R

V

V

L

L

N

M

F

F

C

G

A

S

N
|
N

N

S

Y

Y

L

L

G

G

L

L

S

T

S

T

H

D

P

T

K

R

V

I

I

I

E

K

A

A

A

A

K

Q

N

D

A

A

I

L

D

E

T

K

H

Y

G

G

Y

T

G

G

M

C

S

A

S

G

V

S

R

R

F

F

I

L

C

N

G

G

T

T

Q

L

D

D

I

I

H

H

K

V

E

E

L

L

E

E

R

E

K

K

I

L

S

S

E

A

F

Y

L

V

G

G

T

K

E

E

D

A

T

A

I

I

L

L

Y

F

A

S

A
x
T

A
x
G

F
|
F

D

Q

A

S

N
|
N

G

L

G

G

V

P

F

L

E

S

P

C

I

L

L

M

G

G

P

R

E

N

D

D

A

Y

I

I

I

L

S

L

D

D

A

E

L

R

N

D

H
|
H

A

A

S
|
S

I

I

I

I

D

D

G

G

V

S

R

R

L

L

C

S

K

F

A

S

M

K

R

V

F

I

R

K

Y

Y

K

G

H

H

N

N

D

N

M

M

E

E

D

D

L

L

E

R

T

A

Q

K

L

L

K

S

E

R

A

L

N

P

A

E

K

D

G

S

A

A

K

-

Q

-

K

K

I

L

I

I

V

C

T

T

D
|
D

G

G

A

I

F

F

S

S

M

M

D

E

G

G

T

D

I

I

A

V

Q

N

M

L

D

P

K

E

I

L

V

T

A

S

L

I

A

A

E

N

Q

E

Y

F

D

D

A

A

L

A

V

V

M

M

S

V

D
|
D

E

D

C
x
A

H
|
H

S

S

T

L

G

G

F

V

I

I

G

G

K

H

T

K

G

G

R

A

G

G

V

T

H

A

E

S

L

H

K

F

G

G

V

L

M

N

G

D

K

D

M

V

D

D

I

L

I

I

T

M

G

G

T
|
T

L

F

G
x
S

K
|
K

A

S

L

L

G

-

G

A

A

S

S

L

G

G

G

G

F

F

T

V

S

A

G

G

R

D

K

A

E

D

I

V

I

I

D

D

I

F

L

L

R

K

Q

H

R

N

S

A

R

R

P

S

Y

V

L

M

F

F

S

S

N

A

T

S

L

M

A

T

P

P

A

A

I

S

T

V

G

A

A

S

S

T

I

L

A

K

V

A

F

L

D

E

L

I

L

I

S

Q

E

N

T

E

T

P

E

E

L

H

R

I

D

E

K

K

L

L

E

W

D

K

N

N

T

T

T

D

Y

Y

F

A

R

K

E

A

K

Q

M

L

T

L

A

D

A

H

G

G

F

F

D

D

I

L

K

G

P

A

G

T

V

E

H

S

P

P

I

I

V

L

P

P

I

I

M

F

L

I

Y

R

D

S

A

N

V

E

L

K

S

T

Q

F

Q

W

M

V

A

T

E

K

K

M

L

L

L

Q

E

D

R

D

G

G

V

V

Y

F

V

V

I

N

G

P

F

V

Y

V

Y

S

P

P

V

A

V

V

P

P

K

A

G

E

Q

E

A

S

R

L

I

I

R

R

V

F

Q

S

I

L

S

M

A

A

A

T

H

H

D

T

R

Y

K

D

H

Q

L

I

D

D

A

E

A

A

I

I

E

E

A

K

F

M

T

V

E

K

V

V

G

F

K

K

E

Q

binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-threonine: N51 (= N50), T111 (≠ A110), G112 (≠ A111), F113 (= F112), N116 (= N115), H137 (= H136), S139 (= S138), D180 (= D181), D209 (= D210), A211 (≠ C212), H212 (= H213), T240 (= T241), S242 (≠ G243), K243 (= K244)

8h21A Serine palmitoyltransferase (see paper)
36% identity, 96% coverage: 11:393/397 of query aligns to 13:391/394 of 8h21A

query
sites
8h21A

K

K

E

I

L

V

K

E

E

E

I

L

S

K

D

A

A

K

G

G

L

L

Y

Y

K

A

S

Y

E

F

R

R

I

P

I

I

T

Q

S

S

P

K

Q

Q

G

D

A

T

E

E

I

V

S

K

T

I

S

-

E

D

G

G

K

R

Q

R

V

V

L

L

N

M

F

F

C

G

A

S

N

N

N

S

Y

Y

L

L

G

G

L

L

S

T

S

T

H

D

P

T

K

R

V

I

I

I

E

K

A

A

A

A

K

Q

N

D

A

A

I

L

D

E

T

K

H

Y

G

G

Y

T

G

G

M

C

S

A

S

G

V

S

R

R

F

F

I

L

C

N

G

G

T

T

Q

L

D

D

I

I

H

H

K

V

E

E

L

L

E

E

R

E

K

K

I

L

S

S

E

A

F

Y

L

V

G

G

T

K

E

E

D

A

T

A

I

I

L

L

Y

F

A

S

A
x
T

A
x
G

F
|
F

D

Q

A

S

N
|
N

G

L

G

G

V

P

F

L

E

S

P

C

I

L

L

M

G

G

P

R

E

N

D

D

A

Y

I

I

I

L

S

L

D

D

A

E

L

R

N

D

H
|
H

A

A

S
|
S

I

I

I

I

D

D

G

G

V

S

R

R

L

L

C

S

K

F

A

S

M

K

R

V

F

I

R

K

Y

Y

K

G

H

H

N

N

D

N

M

M

E

E

D

D

L

L

E

R

T

A

Q

K

L

L

K

S

E

R

A

L

N

P

A

E

K

D

G

S

A

A

K

-

Q

-

K

K

I

L

I

I

V

C

T

T

D
|
D

G

G

A

I

F

F

S

S

M

M

D

E

G

G

T

D

I

I

A

V

Q

N

M

L

D

P

K

E

I

L

V

T

A

S

L

I

A

A

E

N

Q

E

Y

F

D

D

A

A

L

A

V

V

M

M

S

V

D
|
D

E

D

C
x
A

H
|
H

S

S

T

L

G

G

F

V

I

I

G

G

K

H

T

K

G

G

R

A

G

G

V

T

H

A

E

S

L

H

K

F

G

G

V

L

M

N

G

D

K

D

M

V

D

D

I

L

I

I

T

M

G

G

T
|
T

L

F

G
x
S

K
|
K

A

S

L

L

G

-

G

A

A

S

S

L

G

G

G

G

F

F

T

V

S

A

G

G

R

D

K

A

E

D

I

V

I

I

D

D

I

F

L

L

R

K

Q

H

R

N

S

A

R

R

P

S

Y

V

L

M

F

F

S

S

N

A

T

S

L

M

A

T

P

P

A

A

I

S

T

V

G

A

A

S

S

T

I

L

A

K

V

A

F

L

D

E

L

I

L

I

S

Q

E

N

T

E

T

P

E

E

L

H

R

I

D

E

K

K

L

L

E

W

D

K

N

N

T

T

T

D

Y

Y

F

A

R

K

E

A

K

Q

M

L

T

L

A

D

A

H

G

G

F

F

D

D

I

L

K

G

P

A

G

T

V

E

H

S

P

P

I

I

V

L

P

P

I

I

M

F

L

I

Y

R

D

S

A

N

V

E

L

K

S

T

Q

F

Q

W

M

V

A

T

E

K

K

M

L

L

L

Q

E

D

R

D

G

G

V

V

Y

F

V

V

I

N

G

P

F

V

Y

V

Y

S

P

P

V

A

V

V

P

P

K

A

G

E

Q

E

A

S

R

L

I

I

R

R

V

F

Q

S

I

L

S

M

A

A

A

T

H

H

D

T

R

Y

K

D

H

Q

L

I

D

D

A

E

A

A

I

I

E

E

A

K

F

M

T

V

E

K

V

V

G

F

K

K

E

Q

binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: T111 (≠ A110), G112 (≠ A111), F113 (= F112), N116 (= N115), H137 (= H136), S139 (= S138), D180 (= D181), D209 (= D210), A211 (≠ C212), H212 (= H213), T240 (= T241), S242 (≠ G243), K243 (= K244)

8h20A Serine palmitoyltransferase (see paper)
36% identity, 96% coverage: 11:393/397 of query aligns to 13:391/394 of 8h20A

query
sites
8h20A

K

K

E

I

L

V

K

E

E

E

I

L

S

K

D

A

A

K

G

G

L

L

Y

Y

K

A

S

Y

E

F

R

R

I

P

I

I

T

Q

S

S

P

K

Q

Q

G

D

A

T

E

E

I

V

S

K

T

I

S

-

E

D

G

G

K

R

Q

R

V

V

L

L

N

M

F

F

C

G

A

S

N

N

N

S

Y

Y

L

L

G

G

L

L

S

T

S

T

H

D

P

T

K

R

V

I

I

I

E

K

A

A

A

A

K

Q

N

D

A

A

I

L

D

E

T

K

H

Y

G

G

Y

T

G

G

M

C

S

A

S

G

V

S

R

R

F

F

I

L

C

N

G

G

T

T

Q

L

D

D

I

I

H

H

K

V

E

E

L

L

E

E

R

E

K

K

I

L

S

S

E

A

F

Y

L

V

G

G

T

K

E

E

D

A

T

A

I

I

L

L

Y

F

A

S

A
x
T

A
x
G

F
|
F

D

Q

A

S

N
|
N

G

L

G

G

V

P

F

L

E

S

P

C

I

L

L

M

G

G

P

R

E

N

D

D

A

Y

I

I

I