SitesBLAST
Comparing Echvi_0146 FitnessBrowser__Cola:Echvi_0146 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5z75A Artificial l-threonine 3-dehydrogenase designed by ancestral sequence reconstruction. (see paper)
65% identity, 94% coverage: 8:314/325 of query aligns to 2:305/306 of 5z75A
- binding nicotinamide-adenine-dinucleotide: G9 (= G15), C11 (≠ A17), G12 (= G18), Q13 (= Q19), I14 (≠ L20), D35 (= D41), I36 (= I42), L49 (= L58), V51 (= V60), L72 (= L81), A73 (= A82), L76 (= L85), P113 (= P122), S115 (= S124), Y140 (= Y149), K144 (= K153), Y167 (= Y176), P168 (= P177), L170 (= L179), P176 (≠ M185), P177 (= P186)
7eprB Partial consensus l-threonine 3-dehydrogenase (c-change) (see paper)
59% identity, 96% coverage: 9:319/325 of query aligns to 2:310/310 of 7eprB
- binding nicotinamide-adenine-dinucleotide: G8 (= G15), G11 (= G18), Q12 (= Q19), I13 (≠ L20), D34 (= D41), I35 (= I42), R36 (≠ N43), D50 (= D59), A51 (≠ V60), L72 (= L81), A73 (= A82), A74 (= A83), L90 (= L99), P113 (= P122), Y140 (= Y149), K144 (= K153), Y167 (= Y176), L170 (= L179)
7cgvA Full consensus l-threonine 3-dehydrogenase, fctdh-iiym (NAD+ bound form) (see paper)
62% identity, 94% coverage: 11:317/325 of query aligns to 3:307/310 of 7cgvA
- binding nicotinamide-adenine-dinucleotide: G7 (= G15), G10 (= G18), Q11 (= Q19), I12 (≠ L20), D33 (= D41), I34 (= I42), R35 (≠ N43), L48 (= L58), D49 (= D59), V50 (= V60), A72 (= A82), A73 (= A83), L75 (= L85), P112 (= P122), S114 (= S124), Y139 (= Y149), K143 (= K153), Y166 (= Y176), I169 (≠ L179)
7epsA Partial consensus l-threonine 3-dehydrogenase (e-change) (see paper)
58% identity, 95% coverage: 11:319/325 of query aligns to 3:309/310 of 7epsA
- binding nicotinamide-adenine-dinucleotide: N9 (≠ A17), G10 (= G18), Q11 (= Q19), I12 (≠ L20), D33 (= D41), V34 (≠ I42), L48 (= L58), A50 (≠ V60), L71 (= L81), A72 (= A82), A73 (= A83), L89 (= L99), P112 (= P122), Y139 (= Y149), K143 (= K153), Y166 (= Y176), L169 (= L179)
- binding threonine: S76 (= S86), S114 (= S124), Y139 (= Y149), G179 (= G189), T180 (= T190), T181 (= T191)
2yy7A Crystal structure of thermolabile l-threonine dehydrogenase from flavobacterium frigidimaris kuc-1 (see paper)
57% identity, 94% coverage: 11:317/325 of query aligns to 5:311/312 of 2yy7A
- binding glycerol: S118 (= S124), Y143 (= Y149), G183 (= G189), T184 (= T190), T185 (= T191), W279 (= W285)
- binding nicotinamide-adenine-dinucleotide: C11 (≠ A17), G12 (= G18), Q13 (= Q19), I14 (≠ L20), D35 (= D41), I36 (= I42), R37 (≠ N43), V52 (≠ L58), N53 (≠ D59), A54 (≠ V60), M75 (≠ L81), A76 (= A82), L93 (= L99), P116 (= P122), S118 (= S124), Y143 (= Y149), K147 (= K153), Y170 (= Y176), L173 (= L179)
3wmwB Gale-like l-threonine dehydrogenase from cupriavidus necator (apo form) (see paper)
53% identity, 94% coverage: 11:317/325 of query aligns to 4:277/278 of 3wmwB
5y1gA Monomeric l-threonine 3-dehydrogenase from metagenome database (akb and nadh bound form) (see paper)
49% identity, 94% coverage: 11:317/325 of query aligns to 4:305/310 of 5y1gA
- binding 2-amino-3-ketobutyric acid: S76 (= S86), G178 (= G189), T179 (= T190), T180 (= T191), W273 (= W285)
- binding nicotinamide-adenine-dinucleotide: G8 (= G15), G11 (= G18), Q12 (= Q19), I13 (≠ L20), D34 (= D41), L35 (≠ I42), D49 (= D59), I50 (≠ V60), L71 (= L81), A72 (= A82), A73 (= A83), L75 (= L85), Y138 (= Y149), K142 (= K153), Y165 (= Y176), L168 (= L179)
5y1fA Monomeric l-threonine 3-dehydrogenase from metagenome database (NAD+ bound form) (see paper)
49% identity, 94% coverage: 11:317/325 of query aligns to 5:306/311 of 5y1fA
- binding nicotinamide-adenine-dinucleotide: G12 (= G18), Q13 (= Q19), I14 (≠ L20), D35 (= D41), L36 (≠ I42), D50 (= D59), I51 (≠ V60), L72 (= L81), A73 (= A82), A74 (= A83), L76 (= L85), S114 (= S124), Y139 (= Y149), K143 (= K153), Y166 (= Y176), L169 (= L179)
5y1eA Monomeric l-threonine 3-dehydrogenase from metagenome database (l-ser and NAD+ bound form) (see paper)
49% identity, 94% coverage: 11:317/325 of query aligns to 2:303/308 of 5y1eA
- binding nicotinamide-adenine-dinucleotide: G6 (= G15), G9 (= G18), Q10 (= Q19), I11 (≠ L20), D32 (= D41), L33 (≠ I42), D47 (= D59), I48 (≠ V60), L69 (= L81), A70 (= A82), A71 (= A83), L73 (= L85), P109 (= P122), Y136 (= Y149), K140 (= K153), Y163 (= Y176), L166 (= L179)
- binding serine: S74 (= S86), S111 (= S124), Y136 (= Y149), G176 (= G189), T177 (= T190), T178 (= T191)
6jygD Crystal structure of l-threonine dehydrogenase from phytophthora infestans (see paper)
47% identity, 96% coverage: 11:321/325 of query aligns to 3:307/307 of 6jygD
- binding citrate anion: Q11 (= Q19), L73 (= L85), S74 (= S86), Y136 (= Y149), G176 (= G189), T178 (= T191), D179 (= D192)
- binding nicotinamide-adenine-dinucleotide: G7 (= G15), T9 (≠ A17), G10 (= G18), Q11 (= Q19), I12 (≠ L20), D33 (= D41), I34 (= I42), K35 (≠ N43), D47 (= D59), V48 (= V60), M69 (≠ L81), A70 (= A82), S71 (≠ A83), L73 (= L85), V87 (≠ L99), P109 (= P122), S110 (= S123), T111 (≠ S124), Y136 (= Y149), K140 (= K153), Y163 (= Y176), V166 (≠ L179)
3a4vA Crystal structure of pyruvate bound l-threonine dehydrogenase from hyperthermophilic archaeon thermoplasma volcanium (see paper)
45% identity, 94% coverage: 11:317/325 of query aligns to 2:305/311 of 3a4vA
- active site: T112 (≠ S124), Y137 (= Y149), K141 (= K153)
- binding nicotinamide-adenine-dinucleotide: S8 (≠ A17), G9 (= G18), Q10 (= Q19), I11 (≠ L20), D32 (= D41), I33 (= I42), L46 (= L58), D47 (= D59), V48 (= V60), L69 (= L81), A70 (= A82), G71 (≠ A83), L73 (= L85), P110 (= P122), S111 (= S123), T112 (≠ S124), Y137 (= Y149), K141 (= K153), Y164 (= Y176), I167 (≠ L179)
- binding pyruvic acid: S74 (= S86), Y137 (= Y149), T178 (= T190), T179 (= T191), W273 (= W285)
3a1nA Crystal structure of l-threonine dehydrogenase from hyperthermophilic archaeon thermoplasma volcanium (see paper)
44% identity, 96% coverage: 11:321/325 of query aligns to 2:309/315 of 3a1nA
- active site: T112 (≠ S124), Y137 (= Y149), K141 (= K153)
- binding nicotinamide-adenine-dinucleotide: S8 (≠ A17), G9 (= G18), Q10 (= Q19), I11 (≠ L20), D32 (= D41), I33 (= I42), L46 (= L58), D47 (= D59), L69 (= L81), A70 (= A82), G71 (≠ A83), L73 (= L85), S111 (= S123), Y137 (= Y149), K141 (= K153), Y164 (= Y176)
3ajrA Crystal structure of l-3-hydroxynorvaline bound l-threonine dehydrogenase (y137f) from hyperthermophilic archaeon thermoplasma volcanium (see paper)
44% identity, 96% coverage: 11:321/325 of query aligns to 2:309/314 of 3ajrA
- binding nicotinamide-adenine-dinucleotide: S8 (≠ A17), G9 (= G18), Q10 (= Q19), I11 (≠ L20), D32 (= D41), I33 (= I42), L46 (= L58), D47 (= D59), V48 (= V60), L69 (= L81), A70 (= A82), G71 (≠ A83), L73 (= L85), P110 (= P122), S111 (= S123), T112 (≠ S124), F137 (≠ Y149), K141 (= K153), Y164 (= Y176), I167 (≠ L179)
- binding (3R)-3-hydroxy-L-norvaline: S74 (= S86), T112 (≠ S124), I113 (= I125), F137 (≠ Y149), G177 (= G189), T178 (= T190), T179 (= T191)
3a9wA Crystal structure of l-threonine bound l-threonine dehydrogenase (y137f) from hyperthermophilic archaeon thermoplasma volcanium (see paper)
44% identity, 96% coverage: 11:321/325 of query aligns to 2:309/315 of 3a9wA
- binding nicotinamide-adenine-dinucleotide: S8 (≠ A17), G9 (= G18), Q10 (= Q19), I11 (≠ L20), D32 (= D41), I33 (= I42), L46 (= L58), D47 (= D59), V48 (= V60), L69 (= L81), A70 (= A82), L73 (= L85), V87 (≠ L99), P110 (= P122), S111 (= S123), K141 (= K153), Y164 (= Y176), I167 (≠ L179)
- binding threonine: S74 (= S86), T112 (≠ S124), F137 (≠ Y149), G177 (= G189), T178 (= T190), T179 (= T191)
Q8K3F7 L-threonine 3-dehydrogenase, mitochondrial; EC 1.1.1.103 from Mus musculus (Mouse) (see paper)
43% identity, 94% coverage: 11:314/325 of query aligns to 58:360/373 of Q8K3F7
- GGLGQL 62:67 (≠ GAAGQL 15:20) binding
- DIR 88:90 (≠ DIN 41:43) binding
- NI 106:107 (≠ DV 59:60) binding
- S133 (= S86) mutation to A: Decreased L-threonine 3-dehydrogenase activity.
- R180 (≠ M134) mutation to K: Decreased L-threonine 3-dehydrogenase activity. No effect on protein NAD(+)-binding. No gross effect on protein folding. No effect on protein stability.
- Y195 (= Y149) binding
- K199 (= K153) binding
- I225 (≠ L179) binding
- T237 (= T191) mutation to A: Decreased L-threonine 3-dehydrogenase activity.
- M333 (≠ D287) mutation to A: Decreased L-threonine 3-dehydrogenase activity.; mutation to E: Decreased L-threonine 3-dehydrogenase activity. Decreased affinity for L-threonine.
4yr9A Mouse tdh with NAD+ bound (see paper)
43% identity, 94% coverage: 11:314/325 of query aligns to 4:305/308 of 4yr9A
- binding glycerol: Y141 (= Y149), T182 (= T191), W276 (= W285)
- binding nicotinamide-adenine-dinucleotide: G11 (= G18), Q12 (= Q19), L13 (= L20), D34 (= D41), I35 (= I42), R36 (≠ N43), A51 (≠ L58), N52 (≠ D59), I53 (≠ V60), Y74 (≠ L81), S75 (≠ A82), A76 (= A83), L78 (= L85), P114 (= P122), T116 (≠ S124), Y141 (= Y149), K145 (= K153), Y168 (= Y176), I171 (≠ L179)
5lc1A L-threonine dehydrogenase from trypanosoma brucei with NAD and the inhibitor pyruvate bound. (see paper)
38% identity, 96% coverage: 8:318/325 of query aligns to 1:312/320 of 5lc1A
- binding nicotinamide-adenine-dinucleotide: G8 (= G15), G11 (= G18), Q12 (= Q19), I13 (≠ L20), D34 (= D41), V35 (≠ I42), D54 (= D59), C55 (≠ V60), L76 (= L81), P77 (≠ A82), A78 (= A83), M80 (≠ L85), P116 (= P122), T118 (≠ S124), Y143 (= Y149), K147 (= K153), L170 (≠ Y176), I173 (≠ L179)
- binding pyruvic acid: S81 (= S86), Y143 (= Y149), G183 (= G189), T185 (= T191), W279 (= W285)
5k4wC Three-dimensional structure of l-threonine 3-dehydrogenase from trypanosoma brucei bound to nadh and l-threonine refined to 1.72 angstroms (see paper)
38% identity, 96% coverage: 8:318/325 of query aligns to 2:313/321 of 5k4wC
- binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G15), G12 (= G18), Q13 (= Q19), I14 (≠ L20), D35 (= D41), V36 (≠ I42), D55 (= D59), C56 (≠ V60), L77 (= L81), P78 (≠ A82), A79 (= A83), M81 (≠ L85), P117 (= P122), Y144 (= Y149), K148 (= K153), L171 (≠ Y176), I174 (≠ L179)
- binding threonine: M81 (≠ L85), S82 (= S86), T119 (≠ S124), Y144 (= Y149), G184 (= G189), A185 (≠ T190), T186 (= T191), W280 (= W285)
5k4qA Three-dimensional structure of l-threonine 3-dehydrogenase from trypanosoma brucei bound to NAD+ refined to 2.3 angstroms (see paper)
38% identity, 96% coverage: 8:318/325 of query aligns to 1:312/320 of 5k4qA
- binding nicotinamide-adenine-dinucleotide: G8 (= G15), L10 (≠ A17), G11 (= G18), Q12 (= Q19), I13 (≠ L20), D34 (= D41), V35 (≠ I42), C55 (≠ V60), L76 (= L81), P77 (≠ A82), A78 (= A83), M80 (≠ L85), P116 (= P122), S117 (= S123), Y143 (= Y149), K147 (= K153), L170 (≠ Y176), I173 (≠ L179)
5k50C Three-dimensional structure of l-threonine 3-dehydrogenase from trypanosoma brucei bound to NAD+ and l-allo-threonine refined to 2.23 angstroms (see paper)
38% identity, 95% coverage: 11:318/325 of query aligns to 3:311/319 of 5k50C
- binding allo-threonine: M79 (≠ L85), S80 (= S86), T117 (≠ S124), Y142 (= Y149), A183 (≠ T190), W278 (= W285)
- binding nicotinamide-adenine-dinucleotide: G7 (= G15), G10 (= G18), Q11 (= Q19), I12 (≠ L20), D33 (= D41), V34 (≠ I42), D53 (= D59), C54 (≠ V60), L75 (= L81), P76 (≠ A82), A77 (= A83), M79 (≠ L85), T117 (≠ S124), Y142 (= Y149), K146 (= K153), L169 (≠ Y176), P170 (= P177), I172 (≠ L179)
Query Sequence
>Echvi_0146 FitnessBrowser__Cola:Echvi_0146
MARLIEIMETILITGAAGQLGSELTLALANMYGGDRIIATDINEASAYKFDYCRFMALDV
MDRKRMTEIVKAEKVTQIYHLAAILSATSEKNPIFAWNLNMESLLSILEIAREQKLNKIY
WPSSIAVFGPNTPMQHTPQDCIMDPNTVYGISKQAGERWCAYYFEKFGVDVRSLRYPGLI
GYKSMPGGGTTDYAVDIFHKAIEGEDFECFLEEDTYLPMMYMDDAIKATLDLMHAPAAQI
KVRSSYNLGGISFSPKEIYECIKQHYPNFNITYKPDFRQQIADTWPDSIDDSAAQNDWGW
QHSYGLEEMTKDILANLPAYLVNLK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory