SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_0156 FitnessBrowser__Cola:Echvi_0156 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q53W83 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; EC 2.7.1.45 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
32% identity, 82% coverage: 26:284/314 of query aligns to 18:279/309 of Q53W83

query
sites
Q53W83

E

E

M

P

G

G

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D

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G

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x
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x
A

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x
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A
x
V

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x
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F

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A

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L

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G

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D

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T

F

H

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Y

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K

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Y

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A

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A

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E

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A

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M

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R

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x
S

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G

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A

A

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A

A

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P

-

Y

-

A

A

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E

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K

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R

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G

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x
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K

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G

G
x
A

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A

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-

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G

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A

G
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G

D
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D

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F

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A

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A

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A

GAEVN 34:38 (≠ SSSAI 42:46) binding substrate
YYR 103:105 (≠ YC- 112:113) binding substrate
R167 (vs. gap) binding substrate
S193 (≠ N193) binding ATP
219:225 (vs. 223:229, 71% identical) binding ATP
GAGD 248:251 (= GAGD 253:256) binding ATP
D251 (= D256) binding substrate
N275 (= N280) binding ATP

Sites not aligning to the query:

287 binding substrate

1v1bA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound atp (see paper)
32% identity, 82% coverage: 26:284/314 of query aligns to 18:279/300 of 1v1bA

query
sites
1v1bA

E

E

M

P

G

G

S

H

E

L

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R

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G

A

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Q

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K

L

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G

G

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G

S

A

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A

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N

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A

A

A

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N

A

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A

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L

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G

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C

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D

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Y

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A

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K

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G

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F

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x
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A

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A

A

N
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N

L

L

T

L

G

G

A
|
A

active site: G248 (= G253), A249 (= A254), G250 (= G255), D251 (= D256)
binding adenosine-5'-triphosphate: K219 (= K223), G221 (= G225), A238 (= A242), F239 (= F243), V241 (≠ N245), G248 (= G253), A249 (= A254), G250 (= G255), N275 (= N280), A279 (= A284)

1v1aA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound 2- keto-3-deoxygluconate and adp (see paper)
32% identity, 82% coverage: 26:284/314 of query aligns to 18:279/301 of 1v1aA

query
sites
1v1aA

E

E

M

P

G

G

S

H

E

L

K

R

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G

A

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K

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Y

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G

G

S
x
G

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x
A

S

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A

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x
N

F

V

A

A

A

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N

A

I

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A

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L

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G

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A

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A

A

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D

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Y

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Y

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x
R

-

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-

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K

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A

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K

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G

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F

F

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A

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A

A

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F

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D

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G
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G

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A

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D
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D

S

A

F

F

N

A

A

A

G

G

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Y

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G

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L

R

R

F

L

A

A

N
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N

L

L

T

L

G

G

A
|
A

active site: G248 (= G253), A249 (= A254), G250 (= G255), D251 (= D256)
binding adenosine-5'-diphosphate: K219 (= K223), G221 (= G225), A222 (≠ V226), A249 (= A254), G250 (= G255), N275 (= N280), A279 (= A284)
binding 2-keto-3-deoxygluconate: G34 (≠ S42), A35 (≠ S43), N38 (≠ I46), Y89 (≠ T98), R105 (vs. gap), R167 (vs. gap), G248 (= G253), D251 (= D256)

Sites not aligning to the query:

binding 2-keto-3-deoxygluconate: 11, 287

3in1A Crystal structure of a putative ribokinase in complex with adp from e.Coli
29% identity, 78% coverage: 48:293/314 of query aligns to 46:295/312 of 3in1A

query
sites
3in1A

A

A

A

T

N

I

I

I

A

S

T

R

L

L

G

G

V

H

D

R

T

T

A

A

F

L

C

M

G

S

M

R

V

I

G

G

K

K

D

D

D

A

F

A

G

G

K

Q

M

F

V

I

Q

L

E

D

T

H

L

C

A

R

A

K

Q

E

Q

N

V

I

D

D

T

I

R

Q

F

S

I

L

A

K

T

Q

S

D

A

V

Q

S

Y

I

K

D

T

T

G

S

L

I

T

N

V

V

-

G

V

L

M

V

N

T

Y

E

D

D

Q

G

D

E

R
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R

A

T

N

F

V

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T

T

-

N

Y

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C

N

G

G

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D

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F

A

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K

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F

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A

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Y

I

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F

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N

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P

G

L

I

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D

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G

K

K

D

A

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T

T

A

E

I

I

F

F

E

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A

A

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C

L

A

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D

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D

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R

D

L

D

N

Q

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D

L

F

D

D

D

Y

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E

C

R

E

C

A

L

L

P

S

H

Y

V

V

D

D

I

Y

F

L

L

F

P

P

N
|
N

E

F

S

A

E

E

L

A

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K

S

L

L

L

T

T

G

G

K

K

S

E

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A

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L

A

E

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A

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K

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K

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G
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G

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K

K

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G
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G

L

C

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F

F

I

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K

K

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G

G

K

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T

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M

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K

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A

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A
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A

F

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V

A

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F

A

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I

D

D

A
x
T

I

I

G
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G

A
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A

G
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G

D
|
D

S

N

F

F

N

A

A

S

G

G

F

F

I

I

H

A

R

A

F

L

L

L

Q

E

G

G

A

K

D

N

W

L

E

R

S

E

C

C

L

A

R

R

F

F

A

A

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N

L

A

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T

G
x
A

A

A

L

I

N

S

T

V

T

L

A

S

A

V

G

G

G

A

T

T

active site: R106 (= R107), G255 (= G253), A256 (= A254), G257 (= G255), D258 (= D256)
binding adenosine-5'-diphosphate: N194 (= N193), K225 (= K223), G227 (= G225), G230 (= G229), A244 (= A242), T253 (≠ A251), N282 (= N280), A285 (≠ G283)

5eynA Crystal structure of fructokinase from vibrio cholerae o395 in fructose, adp, beryllium trifluoride and calcium ion bound form
29% identity, 84% coverage: 41:304/314 of query aligns to 27:303/306 of 5eynA

query
sites
5eynA

G

G

S
x
G

S
x
A

S

P

A

A

I
x
N

F

V

A

A

A

V

N

A

I

I

A

A

T

R

L

L

G

S

V

G

D

R

T

S

A

A

F

F

C

F

G

G

M

R

V

V

G

G

K

N

D

D

D

P

F

F

G

G

K

R

M

F

V

M

Q

Q

E

Q

T

T

L

L

A

T

A

D

Q

E

Q

Q

V

V

D

D

T

C

R

Q

F

H

I

L

A

H

T

F

S

D

A

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V

Y

H

K

R

T

T

G

S

L

-

T

T

V

V

M

V

N

D

Y

L

D

D

Q

E

D
x
H

-

G

R

E

A

R

N

S

V
x
F

T

T

Y
x
F

C

M

-

V

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K

-

P

G

S

A

A

M

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E

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A

F

L

L

T

Q

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L

Q

S

E

D

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P

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F

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G

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E

F

W

K

-

H

-

F

L

H

H

L

V

S

C

N

S

Y

I

F

A

L

L

Q

A

K

N

G

Q

I

P

Q

S

K

R

D

S

I

S

T

T

-

F

A

A

I

A

F

I

E

A

K

Q

A

M

K

K

S

E

A

V

G

G

M

G

T

Y

T

V

S

S

L

F

D

D

L

P

Q

N

V

L

D
x
R

P

-

D

E

Q

V

W

W

D

S

F

E

D

P

Y

Q

E

E

-

L

-

Q

-

A

-

T

-

V

-

M

R

R

C

A

L

V

P

G

H

L

V

A

D

D

I

V

F

V

L

K

P

F

N

S

E

E

S

E

E

E

L

L

L

Q

S

F

L

L

T

T

G

G

K

T

S

Q

S

S

I

I

S

E

E

E

A

G

L

L

E

Q

A

A

I

I

K

A

P

D

Y

F

A

Q

-

I

N

P

T

L

V

V

A

V

L

V

K
x
T

R

L

G
|
G

V
x
A

K

K

G

G

G

A

L

L

V

V

F

A

E

T

K

P

G

N

K

S

V

-

T

Q

E

Q

V

I

A

V

A

S

F

G

V

K

N
x
A

D
x
V

Q

K

F

P

V

I

D

D

A
x
T

I

T

G
|
G

A
|
A

G
|
G

D
|
D

S

A

F
|
F

N

V

A

G

G

G

F

L

I

L

H

Y

R

R

F

L

L

S

Q

V

G

A

A

Q

D

D

W

W

E

H

S

N

-

Q

-

A

-

T

-

I

-

L

-

D

C

A

L

V

R

K

F

W

A

A

N
|
N

L

G

T

C

G
|
G

A
|
A

L

L

N

A

T

T

A

Q

A

K

G

G

G

A

T

M

G

T

A

A

F

L

T

P

S

N

L

Q

A

A

V

L

K

Y

A

A

active site: G246 (= G253), A247 (= A254), G248 (= G255), D249 (= D256)
binding adenosine-5'-diphosphate: H91 (≠ D106), T217 (≠ K223), G219 (= G225), A220 (≠ V226), A238 (≠ N245), V239 (≠ D246), T244 (≠ A251), G246 (= G253), A247 (= A254), G248 (= G255), F251 (= F258), N279 (= N280), G282 (= G283), A283 (= A284)
binding beryllium trifluoride ion: G246 (= G253), G248 (= G255), D249 (= D256)
binding beta-D-fructofuranose: G28 (≠ S42), A29 (≠ S43), N32 (≠ I46), F96 (≠ V110), F98 (≠ Y112), R159 (≠ D171), D249 (= D256)

Sites not aligning to the query:

binding beta-D-fructofuranose: 9, 13

5yggA Crystal structure of fructokinase double-mutant (t261c-h108c) from vibrio cholerae o395 in fructose, adp and potassium ion bound form (see paper)
29% identity, 84% coverage: 41:304/314 of query aligns to 31:307/310 of 5yggA

query
sites
5yggA

G

G

S
x
G

S
x
A

S

P

A

A

I

N

F

V

A

A

A

V

N

A

I

I

A

A

T

R

L

L

G

S

V

G

D

R

T

S

A

A

F

F

C

F

G

G

M

R

V

V

G

G

K

N

D

D

D

P

F

F

G

G

K

R

M

F

V

M

Q

Q

E

Q

T

T

L

L

A

T

A

D

Q

E

Q

Q

V

V

D

D

T

C

R

Q

F

H

I

L

A

H

T

F

S

D

A

P

Q

V

Y

H

K

R

T

T

G

S

L

-

T

T

V

V

M

V

N

D

Y

L

D

D

Q

E

-

C

D

G

R

E

A

R

N

S

V
x
F

T

T

Y
x
F

C

M

-

V

-

K

-

P

G

S

A

A

M

D

E

Q

A

F

L

L

T

Q

M

L

Q

S

E

D

I

I

P

P

W

S

E

F

V

Q

A

K

G

G

T

E

F

W

K

L

H

H

F

V

H

-

L

C

S

S

N

I

Y

A

F

L

L

A

Q

N

K

Q

G

P

I

S

Q

R

K

S

D

S

I

T

T

F

A

A

I

A

F

I

E

A

K

Q

A

M

K

K

S

E

A

V

G

G

M

G

T

Y

T

V

S

S

L

F

D

D

L

P

Q

N

V

L

D
x
R

P

-

D

E

Q

V

W

W

D

S

F

E

D

P

Y

Q

E

E

-

L

-

Q

-

A

-

T

-

V

-

M

R

R

C

A

L

V

P

G

H

L

V

A

D

D

I

V

F

V

L
x
K

P

F

N

S

E

E

S

E

E

E

L

L

L

Q

S

F

L

L

T

T

G

G

K

T

S

Q

S

S

I

I

S

E

E

E

A

G

L

L

E

Q

A

A

I

I

K

A

P

D

Y

F

A

Q

-

I

N

P

T

L

V

V

A

V

L

V

K
x
T

R

L

G
|
G

V

A

K

K

G

G

G

A

L

L

V

V

F

A

E

T

K

P

G

N

K

S

V

-

T

Q

E

Q

V

I

A

V

A

S

F

G

V

K

N
x
A

D
x
V

Q

K

F

P

V

I

D

D

A

C

I

T

G

G

A

A

G

G

D
|
D

S

A

F
|
F

N

V

A

G

G

G

F

L

I

L

H

Y

R

R

F

L

L

S

Q

V

G

A

A

Q

D

D

W

W

E

H

S

N

-

Q

-

A

-

T

-

I

-

L

-

D

C

A

L

V

R

K

F

W

A

A

N
|
N

L

G

T

C

G
|
G

A
|
A

L

L

N

A

T

T

A

Q

A

K

G

G

G

A

T

M

G

T

A

A

F

L

T

P

S

N

L

Q

A

A

V

L

K

Y

A

A

binding adenosine-5'-diphosphate: K188 (≠ L191), T221 (≠ K223), G223 (= G225), A242 (≠ N245), V243 (≠ D246), F255 (= F258), N283 (= N280), G286 (= G283), A287 (= A284)
binding beta-D-fructofuranose: G32 (≠ S42), A33 (≠ S43), F100 (≠ V110), F102 (≠ Y112), R163 (≠ D171), D253 (= D256)

Sites not aligning to the query:

binding beta-D-fructofuranose: 13, 17

6ilsB Structure of arabidopsis thaliana ribokinase complexed with ribose and atp (see paper)
29% identity, 91% coverage: 6:291/314 of query aligns to 5:294/313 of 6ilsB

query
sites
6ilsB

L

L

L

V

V

V

G

G

E

S

L

A

N
|
N

I

A

D
|
D

L

I

I

Y

L

V

N

-

Q

E

I

I

Q

E

G

R

F

L

P

P

E

K

M

E

G

G

S

E

E

T

K

I

V

S

A

A

Q

K

K

T

M

G

D

Q

V

T

V

L

L

A

G

G

S
x
G

S
x
K

S

G

A

A

I
x
N

F

Q

A

A

N

C

I

G

A

A

T

K

L

L

G

M

V

Y

D

P

T

T

A

Y

F

F

C

V

G

G

M

R

V

L

G

G

K

E

D

D

D

A

F

H

G

G

K

K

M

L

V

I

Q

A

E

E

T

A

L

L

A

G

-

D

A

G

Q

C

Q

G

V

V

D

H

T

L

R

D

F

Y

I

V

A

R

T

S

S

V

A

N

Q

N

Y

E

K

P

T

T

G

G

L

H

T

A

V

V

M

M

N

L

Y

Q

D

S

Q

D

D

G

R

Q

A

N

N

S

V

I

T

I

Y

I

C

V

G

G

A

G

M

A

E

N

A

M

L

K

T

A

M

W

Q

P

E

E

I

I

-

M

-

S

-

D

P

D

W

L

E

E

V

I

A

V

G

-

T

-

F

-

K

-

H

-

F

R

H

N

L

A

S

G

N

I

Y

V

F

L

L

L

Q

Q

K

R

G
x
E

I

I

Q

P

K

D

D

S

I

I

T

N

A

I

I

Q

F

V

E

A

K

K

A

A

-

V

K

K

S

K

A

A

G

G

M

V

T

P

T

V

S

I

L

L

D

D

L

V

-

G

-

M

-

D

Q

T

V

P

D

I

P

P

D

N

D

E

Q

L

W

L

D

D

F

-

D

-

Y

-

E

-

R

-

C

-

L

-

P

-

H

S

V

I

D

D

I

I

F

L

L

S

P

P

N
|
N

E

E

S

T

E

E

L

L

L

S

S

R

L

L

T

T

G

G

K

M

S

P

S

T

-

E

-

T

-

F

-

E

-

Q

I

I

S

S

E

Q

A

A

L

V

-

A

E

K

A

C

I

H

K

K

P

L

Y

G

A

V

N

K

T

Q

V

V

A

L

L

V

K
|
K

R

L

G
|
G

V

S

K

K

G

G

G

S

L

A

V

L

F

F

E

I

K

Q

G

G

-

E

K

K

V

P

T

I

E

Q

V

Q

A

S

A

I

F
x
I

V

P

N
x
A

D

A

Q

Q

F

V

V

V

D

D

A

T

I

T

G

G

A
|
A

G
|
G

D
|
D

S

T

F
|
F

N

T

A

A

G

A

F

F

I

A

H

V

R

A

F

M

L

V

Q

E

G

G

A

K

D

S

W

H

E

E

S

E

C

C

L

L

R

R

F

F

A

A

N

A

L

A

T

A

G
x
A

A
x
S

L

L

N

C

T

V

T

Q

A

V

A

K

G

G

binding adenosine-5'-triphosphate: N189 (= N193), K225 (= K223), G227 (= G225), I246 (≠ F243), A248 (≠ N245), A257 (= A254), G258 (= G255), F261 (= F258), A286 (≠ G283), S287 (≠ A284)
binding alpha-D-ribofuranose: N12 (= N13), D14 (= D15), G40 (≠ S42), K41 (≠ S43), N44 (≠ I46), E144 (≠ G145), D259 (= D256)

A1A6H3 Ribokinase; AtRBSK; RK; EC 2.7.1.15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 95% coverage: 6:302/314 of query aligns to 71:371/379 of A1A6H3

query
sites
A1A6H3

L
|
L

L
x
V

V
|
V

G

G

E

S

L

A

N

N

I

A

D

D

L

I

I

Y

L

V

N

-

Q

E

I

I

Q

E

G

R

F

L

P

P

E

K

M

E

G

G

S

E

E

T

K

I

V

S

A

A

Q

K

K

T

M

G

D

Q

V

T

V

L

L

A

G

G

S

G

S

K

S

G

A

A

I

N

F

Q

A

A

N

C

I

G

A

A

T

K

L

L

G

M

V

Y

D

P

T

T

A

Y

F

F

C

V

G

G

M

R

V

L

G

G

K

E

D

D

D

A

F

H

G

G

K

K

M

L

V

I

Q

A

E

E

T

A

L

L

A

G

-

D

A

G

Q

C

Q

G

V

V

D

H

T

L

R

D

F

Y

I

V

A

R

T

S

S

V

A

N

Q

N

Y

E

K

P

T

T

G

G

L

H

T

A

V

V

M

M

N

L

Y

Q

D

S

Q

D

D

G

R

Q

A

N

N

S

V

I

T

I

Y

I

C

V

G

G

A

G

M

A

E

N

A

M

L

K

T

A

M

W

Q

P

E

E

I

I

-

M

-

S

-

D

P

D

W

L

E

E

V

I

A

V

G

-

T

-

F

-

K

-

H

-

F

R

H

N

L

A

S

G

N

I

Y

V

F

L

L

L

Q

Q

K

R

G

E

I

I

Q

P

K

D

D

S

I

I

T

N

A

I

I

Q

F

V

E

A

K

K

A

A

-

V

K

K

S

K

A

A

G

G

M

V

T

P

T

V

S

I

L

L

D

D

L

V

-

G

-

M

-

D

Q

T

V

P

D

I

P

P

D

N

D

E

Q

L

W

L

D

D

F

-

D

-

Y

-

E

-

R

-

C

-

L

-

P

-

H

S

V

I

D

D

I

I

F

L

L

S

P

P

N

N

E

E

S

T

E

E

L

L

L

S

S

R

L

L

T

T

G

G

K

M

S

P

S

T

-

E

-

T

-

F

-

E

-

Q

I

I

S

S

E

Q

A

A

L

V

-

A

E

K

A

C

I

H

K

K

P

L

Y

G

A

V

N

K

T

Q

V

V

A

L

L

V

K

K

R

L

G

G

V

S

K

K

G

G

G

S

L

A

V

L

F

F

E

I

K

Q

G

G

-

E

K

K

V

P

T

I

E

Q

V

Q

A

S

A

I

F

I

V

P

N

A

D

A

Q

Q

F

V

V

V

D

D

A

T

I

T

G

G

A

A

G

G

D

D

S

T

F

F

N

T

A

A

G

A

F

F

I

A

H

V

R

A

F

M

L

V

Q

E

G

G

A

K

D

S

W

H

E

E

S

E

C

C

L

L

R

R

F

F

A

A

N

A

L

A

T

A

G

A

A

S

L

L

N

C

T

V

T

Q

A

V

A

K

G

G

G

A

T

I

G

P

A

S

F

M

T

P

S

D

L

R

A

K

A

S

V

V

Sites not aligning to the query:

1:74 mutation Missing: Loss of ribokinase activity.

4xckA Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
26% identity, 95% coverage: 6:304/314 of query aligns to 4:300/306 of 4xckA

query
sites
4xckA

L

L

L

V

V

V

V

L

G

G

E

S

L

V

N
|
N

I

A

D
|
D

L

H

I

V

L

L

N

-

Q

Q

I

V

Q

P

G

S

F

F

P

P

E

R

M

P

G

G

S

E

E

T

K

L

V

H

A

G

Q

R

K

N

M

Y

D

Q

V

V

V

I

L

P

G

G

S
x
G

S
x
K

S

G

A

A

I
x
N

F

Q

A

A

A

V

N

A

I

A

A

A

T

R

L

M

G

Q

V

A

D

D

T

V

A

G

F

F

C

I

G

A

M

C

V

V

G

G

K

D

D

D

D

S

F

F

G

G

K

I

M

N

V

I

Q

R

E

E

T

S

L

F

A

K

A

L

Q

D

Q

G

V

I

D

N

T

T

R

A

F

G

I

V

A

K

T

L

S

Q

A

P

Q

N

Y

C

K

P

T

T

G

G

L

I

T
x
A

V

M

V

I

M

Q

N

V

Y

S

D

D

Q

S

D

G

R

E

A

N

N

S

V
x
I

T

-

Y

-

C

C

G
x
I

A

S

M

A

E

E

A

A

-

N

-

A

-

K

L

L

T

T

M

A

Q

A

E

A

I

I

P

E

W

P

E

D

V

L

A

A

G

A

T

-

F

-

K

-

H

-

F

I

H

R

L

D

S

A

N

R

Y

Y

F

L

L

L

Q

M

K

Q

G

-

I

L

Q
x
E

K

T

D

P

I

L

T

D

A

G

I

I

F

L

E

K

K

A

A

A

K

Q

S

E

A

A

G

K

M

T

T

A

-

K

T

T

S

N

L

V

D

I

L

L

Q

N

V

P

D

A

P

P

D

A

D

R

Q

E

W

L

D

P

F

D

D

E

Y

L

E

L

R

K

C

C

L

-

P

-

H

-

V

V

D

D

I

L

F

I

L

T

P

P

N

N

E

E

S

T

E

E

L

A

L

E

S

V

L

L

T

T

G

G

-

I

-

T

-

V

-

Y

-

D

K

D

S

S

I

A

S

Q

E

Q

A

A

L

A

E

D

A

A

I

L

K

H

P

C

Y

K

A

G

-

I

N

E

T

I

V

V

A

I

L

I

K
x
T

R

L

G
|
G

V
x
S

K

K

G

G

G

V

L

W

V

L

F

S

E

Q

K

N

G

G

K

R

V

G

T

Q

E

R

V

I

A

P

A

G

F

F

V

V

N
x
V

D

-

Q

K

F

A

V

T

D

D

A
x
T

I
x
T

G
x
A

A
|
A

G
|
G

D
|
D

S

T

F
|
F

N

N

A

G

G

A

F

L

I

V

H

T

R

G

F

L

L

L

Q

Q

G

E

A

M

D

P

W

L

E

E

S

S

C

A

L

I

R

K

F

F

A

A

N
x
H

L

A

T

A

G
x
A

A

A

L

I

N

S

T
x
V

T

T

A

R

A

F

G

G

G

A

T

Q

G

T

A

S

F

I

T

P

S

T

L

R

A

A

A

E

V

V

K

E

A

A

active site: A249 (≠ G253), A250 (= A254), G251 (= G255), D252 (= D256)
binding adenosine-5'-diphosphate: T220 (≠ K223), G222 (= G225), S223 (≠ V226), V242 (≠ N245), T247 (≠ A251), A250 (= A254), F254 (= F258), H276 (≠ N280), A279 (≠ G283), V283 (≠ T287)
binding alpha-D-ribofuranose: N11 (= N13), D13 (= D15), G39 (≠ S42), K40 (≠ S43), N43 (≠ I46), A95 (≠ T98), I107 (≠ V110), I109 (≠ G114), E140 (≠ Q147), T248 (≠ I252), D252 (= D256)

3iq0B Crystal structure of a putative ribokinase ii in complex with atp and mg+2 from e.Coli
28% identity, 79% coverage: 43:291/314 of query aligns to 37:290/308 of 3iq0B

query
sites
3iq0B

S

A

S

P

A

A

I

I

F

F

A

I

A

D

N

Q

I

V

A

T

T

R

L

L

G

G

V

V

D

P

T

C

A

G

F

I

C

I

G

S

M

C

V

V

G

G

K

N

D

D

D

G

F

F

G

G

K

D

M

I

V

N

Q

I

E

H

T

R

L

L

A

A

Q

D

Q

G

V

V

D

D

T

I

R

R

F

G

I

I

A

S

T

V

S

L

A

P

Q

L

Y

E

K

A

T

T

G

G

L

S

T

A

V

F

V

V

M

T

N

Y

Y

G

D

D

Q

R

D

D

R

F

A

I

N

F

V

N

T

I

Y

K

C

N

G

A

A

A

M

C

E

G

A

K

L

L

T

S

M

A

Q

Q

E

H

I

V

P

D

W

E

E

N

V

I

A

L

G

K

T

D

F

C

K

T

H

H

F

F

H

H

-

I

-

M

-

G

-

S

L

L

S

F

N

S

Y

F

F

H

L

M

Q

V

K

D

G

A

I

V

Q

K

K

K

D

A

I

V

T

T

A

I

I

V

F

-

E

-

K

-

A

-

K

K

S

A

A

N

G

G

M

G

T

V

T

I

S

S

L

F

D

D

L

P

Q

N

V

I

D

R

P

-

D

K

D

E

Q

M

W

L

D

D

F

I

D

P

Y

E

E

M

R

R

C

D

L

A

P

L

H

H

-

F

-

V

-

L

-

E

-

L

V

T

D

D

I

I

F

Y

L

M

P

P

N
x
S

E

E

S

G

E

E

L

V

L

L

S

L

L

L

T

S

G

P

K

H

S

S

S

T

I

P

S

E

E

R

A

A

L

I

E

A

A

G

-

F

I

L

K

E

P

E

Y

G

A

V

N

K

T

E

V

V

A

I

L

V

K
|
K

R

R

G
|
G

V

N

K

Q

G

G

G

A

L

S

V

Y

F

Y

E

S

K

A

G

N

K

E

V

Q

T

F

E

H

V

V

A

E

A

S

F

Y

V

P

N

V

D

E

Q
x
E

F

-

V

V

D

D

A

P

I

T

G
|
G

A
|
A

G
|
G

D
|
D

S

C

F
|
F

N

G

A

G

G

A

F

W

I

I

H

A

R

C

F

R

L

Q

Q

L

G

G

A

F

D

D

W

A

E

H

S

R

C

A

L

L

R

Q

F

Y

A

A

N
|
N

L

A

T

C

G
|
G

A

A

L

L

N

A

T

V

T

T

A

R

G

G

active site: G252 (= G253), A253 (= A254), G254 (= G255), D255 (= D256)
binding adenosine-5'-triphosphate: S192 (≠ N193), K223 (= K223), G225 (= G225), E247 (≠ Q247), A253 (= A254), G254 (= G255), F257 (= F258), N279 (= N280), G282 (= G283)

8cqxA Ribokinase from t.Sp mutant a92g
28% identity, 91% coverage: 6:291/314 of query aligns to 2:283/300 of 8cqxA

query
sites
8cqxA

L

I

L

L

V

V

G

G

E

S

L

L

N

N

I

M

D

D

L

L

I

V

L

L

N

-

Q

R

I

V

Q

K

G

R

F

L

P

P

E

R

M

P

G

G

S

E

E

T

K

V

V

L

A

G

Q

E

K

D

M

Y

D

Q

V

T

V

H

L

P

G

G

S

G

S

K

S

G

A

A

I

N

F

Q

A

A

A

V

N

A

I

I

A

A

T

R

L

L

G

G

V

G

D

K

T

V

A

R

F

M

C

L

G

G

M

R

V

V

G

G

K

E

D

D

D

P

F

F

G

G

K

Q

M

A

V

L

Q

K

E

S

T

G

L

L

A

A

A

Q

Q

E

Q

G

V

V

D

D

T

V

R

A

F

W

I

V

-

L

A

E

T

T

S

P

A

G

Q

P

Y

S

K

G

T

T

G

G

L

F

T

I

V

L

V

V

M

D

N

P

Y

E

D

G

Q

Q

D

N

R

Q

A

I

N

A

V

V

T

A

Y

-

C

P

G

G

A

A

M

N

E

A

A

R

L

L

T

V

M

P

Q

E

E

D

I

L

P

P

W

-

E

-

V

-

A

A

G

T

T

A

F

F

K

Q

H

G

F

V

H

G

L

V

S

V

N

L

Y

L

F

Q

L

L

Q

E

K

I

G

P

I

L

Q

E

K

T

D

V

I

V

-

R

-

A

T

A

A

A

I

L

F

G

E

R

K

K

A

A

K

G

S

A

A

R

G

I

M

L

T

L

T

N

S

A

L

A

D

P

L

A

Q

H

V

A

D

L

P

P

D

S

D

E

Q

-

W

-

D

-

F

-

D

-

Y

-

E

-

R

-

C

I

L

L

P

Q

H

S

V

V

D

D

I

L

F

L

L

L

P

V

N
|
N

E

E

S

V

E

E

L

A

S

Q

L

L

T

T

G

E

K

A

S

S

S

P

I

P

S

R

E

T

A

P

L

E

E

E

A

A

I

L

-

A

-

L

-

A

-

R

-

Q

-

L

-

R

-

G

-

R

K

A

P

P

Y

Q

A

A

N

Q

T

V

V

V

A

-

L

L

K
x
T

R

L

G
|
G

V
x
A

K

Q

G
|
G

G

A

L

-

V

V

F

W

E

S

K

G

G

T

K

E

V

E

T

S

E

H

V

F

A

P

A

A

F

F

V

-

N

P

D

V

Q

R

F

A

V

V

D
|
D

A

T

I
x
T

G

A

A

A

G

G

D

D

S

A

F
|
F

N

A

A

G

G

A

F

L

I

A

H

L

R

G

F

L

L

A

Q

E

G

G

A

Q

D

N

W

M

E

R

S

A

C

A

L

L

R

R

F

F

A

A

N
|
N

L

A

T

A

G
|
G

A
|
A

L

L

N
x
A

T
|
T

T

T

A

R

A

P

G

G

binding adenosine-5'-diphosphate: N179 (= N193), T217 (≠ K223), G219 (= G225), A220 (≠ V226), G222 (= G228), F250 (= F258), N272 (= N280), G275 (= G283), A276 (= A284), T279 (= T287)
binding magnesium ion: D242 (= D250), T244 (≠ I252), A278 (≠ N286)

Sites not aligning to the query:

binding magnesium ion: 287

3uboA The crystal structure of adenosine kinase from sinorhizobium meliloti
27% identity, 78% coverage: 41:285/314 of query aligns to 57:306/338 of 3uboA

query
sites
3uboA

G
|
G

S
x
G

S
|
S

S

A

A

G

I
x
N

F

T

A

A

N

G

I

V

A

A

T

S

L

L

G

G

V

G

D

R

T

A

A

A

F

Y

C

F

G

G

M

K

V

V

G

A

K

D

D

D

D

Q

F

L

G

G

K

E

M

I

V

F

Q

T

E

H

T

D

L

I

A

R

A

A

Q

Q

Q

G

V

V

D

H

T

F

R

Q

F

T

I

K

A

P

T

L

S

D

A

G

Q

H

Y

P

K

P

T

T

G

A

L

R

T

S

V

M

V

I

-

F

M

V

N

T

Y

E

D

D

Q

G

D

E

R
|
R

A

S

N
x
M

V

N

T
|
T

Y

Y

C

L

G

G

A

A

M

C

E

V

A

E

L

L

T

G

M

P

Q

E

E

D

I

V

P

E

W

D

E

D

V

V

A

V

G

A

T

Q

F

S

K

K

H

V

F

T

H

Y

L

F

S
x
E

N

G

Y
|
Y

F

L

L

W

Q

D

K

P

G

P

I

R

Q

A

K

K

D

D

I

-

T

-

A

A

I

I

F

R

E

E

K

A

A

A

K

R

S

I

A

A

-

H

-

A

-

H

-

G

-

R

-

E

-

T

G

A

M

M

T

T

T

L

S

S

L

D

D

S

L

F

Q

C

V

V

D

H

P

R

D

Y

D

R

Q

S

W

E

D

F

F

L

D

E

Y

L

E

M

R

R

C

S

L

-

P

G

H

T

V

V

D

D

I

I

F

V

L

F

P

A

N
|
N

E

R

S

Q

E

E

L

A

L

L

S

A

L

L

T

Y

G

E

K

T

S

E

S

D

I

F

S

D

E

R

A

A

L

L

E

E

A

L

I

L

K

A

P

R

Y

D

A

C

N

K

T

L

V

A

A

A

L

V

K
x
T

R

L

G
x
S

V
x
E

K

E

G
|
G

G

S

L

V

V

V

F

V

E

R

K

G

G

A

K

E

V

R

T

V

E

R

V

V

A

G

A

A

F

S

V

V

N
x
L

D

E

Q

Q

F

V

V

V

D

D

A

T

I

T

G
|
G

A
|
A

G
|
G

D
|
D

S

L

F

Y

N

A

A

A

G

G

F

F

I

L

H

F

R

G

F

Y

L

T

Q

S

G

G

A

R

D

S

W

L

E

E

S

E

C

C

L

S

R

K

F

L

A

G

N
|
N

L

L

T

A

G
x
A

A

G

L

I

active site: R124 (= R107), G274 (= G253), A275 (= A254), G276 (= G255), D277 (= D256)
binding adenosine: G57 (= G41), G58 (≠ S42), S59 (= S43), N62 (≠ I46), M126 (≠ N109), T128 (= T111), E155 (≠ S138), Y157 (= Y140), G274 (= G253), D277 (= D256)
binding adenosine-5'-diphosphate: N214 (= N193), T244 (≠ K223), S246 (≠ G225), E247 (≠ V226), G249 (= G228), L266 (≠ N245), A275 (= A254), G276 (= G255), N301 (= N280), A304 (≠ G283)

Sites not aligning to the query:

1gqtB Activation of ribokinase by monovalent cations (see paper)
26% identity, 84% coverage: 6:268/314 of query aligns to 6:266/307 of 1gqtB

query
sites
1gqtB

L

L

L

V

V

V

V

L

G

G

E

S

L

I

N
|
N

I

A

D
|
D

L

H

I

I

L

L

N

N

Q

-

I

L

Q

Q

G

S

F

F

P

P

E

T

M

P

G

G

S

E

E

T

K

V

V

T

A

G

Q

N

K

H

M

Y

D

Q

V

V

V

A

L

F

G

G

S
x
G

S

K

S

G

A

A

I
x
N

F

Q

A

A

A

V

N

A

I

A

A

G

T

R

L

S

G

G

V

A

D

N

T

I

A

A

F

F

C

I

G

A

M

C

V

T

G

G

K

D

D

D

D

S

F

I

G

G

K

E

M

S

V

V

Q

R

E

Q

T

Q

L

L

A

A

A

T

Q

D

Q

N

V

I

D

D

T

I

R

T

F

P

I

V

A

S

T

V

S

I

A

K

Q

G

Y

E

K

S

T

T

G

G

L

V

T

A

V

L

V

I

-

F

M

V

N

N

Y

G

D

E

Q

G

D

E

R

N

A

V

N

I

V

G

T

I

Y

H

C

A

G

G

A

A

M

N

E

A

A

A

L

L

T

S

M

-

Q

-

E

-

I

-

P

P

W

A

E

L

V

V

A

E

G

A

T

Q

F

R

K

E

H

R

F

I

H

A

L

N

S

A

N

S

Y

A

F

L

L

L

Q

M

K

Q

G

-

I

L

Q
x
E

K

S

D

P

I

L

T

E

A

S

I

V

F

M

E

A

K

A

A

A

K

K

S

I

A

A

G

H

M

Q

T

N

T

K

S

T

L

I

D

-

L

V

Q

A

V

L

D

N

P

P

D

A

D

P

Q

A

W

R

D

E

F

L

D

P

Y

D

E

E

R

-

C

L

L

L

P

A

H

L

V

V

D

D

I

I

F

I

L

T

P

P

N
|
N

E

E

S

T

E

E

L

A

L

E

S

K

L

L

T

T

G

G

-

I

-

R

-

V

-

E

-

N

K

D

S

E

S

D

I

A

S

A

E

K

A

A

L

A

E

Q

A

V

I

L

K

H

P

E

Y

K

A

G

-

I

N

R

T

T

V

V

A

L

L

I

K
x
T

R

L

G
|
G

V
x
S

K

R

G

G

G

V

L

W

V

A

F

S

E

V

K

N

G

G

K

E

V

G

T

Q

E

R

V

V

A

P

A

G

F

F

V

-

N

R

D

V

Q

Q

F

A

V

V

D
|
D

A

T

I
|
I

G
x
A

A
|
A

G
|
G

D
|
D

S

T

F

F

N

N

A

G

G

A

F

L

I

I

H

T

R

A

F

L

L

L

Q

E

active site: A251 (≠ G253), A252 (= A254), G253 (= G255), D254 (= D256)
binding phosphomethylphosphonic acid adenylate ester: N186 (= N193), T222 (≠ K223), G224 (= G225), S225 (≠ V226), A252 (= A254), G253 (= G255)
binding cesium ion: D248 (= D250), I250 (= I252)
binding alpha-D-ribofuranose: N13 (= N13), D15 (= D15), G41 (≠ S42), N45 (≠ I46), E142 (≠ Q147), D254 (= D256)

Sites not aligning to the query:

P0A9J6 Ribokinase; RK; EC 2.7.1.15 from Escherichia coli (strain K12) (see 3 papers)
26% identity, 84% coverage: 6:268/314 of query aligns to 7:267/309 of P0A9J6

query
sites
P0A9J6

L

L

L

V

V

V

V

L

G

G

E

S

L

I

N
|
N

I
x
A

D
|
D

L

H

I

I

L

L

N

N

Q

-

I

L

Q

Q

G

S

F

F

P

P

E

T

M

P

G

G

S

E

E

T

K

V

V

T

A

G

Q

N

K

H

M

Y

D

Q

V

V

V

A

L

F

G

G

S
x
G

S
x
K

S
x
G

A
|
A

I
x
N

F

Q

A

A

A

V

N

A

I

A

A

G

T

R

L

S

G

G

V

A

D

N

T

I

A

A

F

F

C

I

G

A

M

C

V

T

G

G

K

D

D

D

D

S

F

I

G

G

K

E

M

S

V

V

Q

R

E

Q

T

Q

L

L

A

A

A

T

Q

D

Q

N

V

I

D

D

T

I

R

T

F

P

I

V

A

S

T

V

S

I

A

K

Q

G

Y

E

K

S

T

T

G

G

L

V

T

A

V

L

V

I

-

F

M

V

N

N

Y

G

D

E

Q

G

D

E

R

N

A

V

N

I

V

G

T

I

Y

H

C

A

G

G

A

A

M

N

E

A

A

A

L

L

T

S

M

-

Q

-

E

-

I

-

P

P

W

A

E

L

V

V

A

E

G

A

T

Q

F

R

K

E

H

R

F

I

H

A

L

N

S

A

N

S

Y

A

F

L

L

L

Q

M

K

Q

G

-

I

L

Q
x
E

K

S

D

P

I

L

T

E

A

S

I

V

F

M

E

A

K

A

A

A

K

K

S

I

A

A

G

H

M

Q

T

N

T

K

S

T

L

I

D

-

L

V

Q

A

V

L

D

N

P

P

D

A

D

P

Q

A

W

R

D

E

F

L

D

P

Y

D

E

E

R

-

C

L

L

L

P

A

H

L

V

V

D

D

I

I

F

I

L

T

P

P

N
|
N

E

E

S

T

E

E

L

A

L

E

S

K

L

L

T

T

G

G

-

I

-

R

-

V

-

E

-

N

K

D

S

E

S

D

I

A

S

A

E

K

A

A

L

A

E

Q

A

V

I

L

K

H

P

E

Y

K

A

G

-

I

N

R

T

T

V

V

A

L

L

I

K
x
T

R
x
L

G
|
G

V
x
S

K
x
R

G
|
G

G

V

L

W

V

A

F

S

E

V

K

N

G

G

K

E

V

G

T

Q

E

R

V

V

A

P

A

G

F

F

V

-

N

R

D

V

Q

Q

F

A

V

V

D

D

A

T

I

I

G

A

A

A

G
|
G

D
|
D

S

T

F

F

N

N

A

G

G

A

F

L

I

I

H

T

R

A

F

L

L

L

Q

E

NAD 14:16 (≠ NID 13:15) binding substrate
GKGAN 42:46 (≠ SSSAI 42:46) binding substrate
E143 (≠ Q147) binding substrate
N187 (= N193) binding ATP
TLGSRG 223:228 (≠ KRGVKG 223:228) binding ATP
GD 254:255 (= GD 255:256) binding ATP
D255 (= D256) binding substrate

Sites not aligning to the query:

279 binding ATP

1rk2A E. Coli ribokinase complexed with ribose and adp, solved in space group p212121 (see paper)
26% identity, 84% coverage: 6:268/314 of query aligns to 4:264/305 of 1rk2A

query
sites
1rk2A

L

L

L

V

V

V

V

L

G

G

E

S

L

I

N
|
N

I

A

D
|
D

L

H

I

I

L

L

N

N

Q

-

I

L

Q

Q

G

S

F

F

P

P

E

T

M

P

G

G

S

E

E

T

K

V

V

T

A

G

Q

N

K

H

M

Y

D

Q

V

V

V

A

L

F

G
|
G

S
x
G

S
x
K

S

G

A

A

I
x
N

F

Q

A

A

A

V

N

A

I

A

A

G

T

R

L

S

G

G

V

A

D

N

T

I

A

A

F

F

C

I

G

A

M

C

V

T

G

G

K

D

D

D

D

S

F

I

G

G

K

E

M

S

V

V

Q

R

E

Q

T

Q

L

L

A

A

A

T

Q

D

Q

N

V

I

D

D

T

I

R

T

F

P

I

V

A

S

T

V

S

I

A

K

Q

G

Y

E

K

S

T

T

G

G

L

V

T

A

V

L

V

I

-

F

M

V

N

N

Y

G

D

E

Q

G

D

E

R

N

A

V

N

I

V

G

T

I

Y

H

C

A

G

G

A

A

M

N

E

A

A

A

L

L

T

S

M

-

Q

-

E

-

I

-

P

P

W

A

E

L

V

V

A

E

G

A

T

Q

F

R

K

E

H

R

F

I

H

A

L

N

S

A

N

S

Y

A

F

L

L

L

Q

M

K

Q

G

-

I

L

Q
x
E

K

S

D

P

I

L

T

E

A

S

I

V

F

M

E

A

K

A

A

A

K

K

S

I

A

A

G

H

M

Q

T

N

T

K

S

T

L

I

D

-

L

V

Q

A

V

L

D

N

P

P

D

A

D

P

Q

A

W

R

D

E

F

L

D

P

Y

D

E

E

R

-

C

L

L

L

P

A

H

L

V

V

D

D

I

I

F

I

L

T

P

P

N

N

E

E

S

T

E

E

L

A

L

E

S

K

L

L

T

T

G

G

-

I

-

R

-

V

-

E

-

N

K

D

S

E

S

D

I

A

S

A

E

K

A

A

L

A

E

Q

A

V

I

L

K

H

P

E

Y

K

A
x
G

-

I

N
x
R

T

T

V

V

A

L

L

I

K
x
T

R

L

G
|
G

V
x
S

K

R

G

G

G

V

L

W

V

A

F

S

E

V

K

N

G

G

K

E

V

G

T

Q

E

R

V

V

A

P

A

G

F

F

V

-

N

R

D

V

Q

Q

F

A

V

V

D
|
D

A

T

I

I

G
x
A

A
|
A

G
|
G

D
|
D

S

T

F

F

N

N

A

G

G

A

F

L

I

I

H

T

R

A

F

L

L

L

Q

E

active site: A249 (≠ G253), A250 (= A254), G251 (= G255), D252 (= D256)
binding adenosine-5'-diphosphate: T220 (≠ K223), G222 (= G225), S223 (≠ V226), A250 (= A254), G251 (= G255)
binding tetrafluoroaluminate ion: G213 (≠ A217), R215 (≠ N218)
binding magnesium ion: D246 (= D250)
binding alpha-D-ribofuranose: N11 (= N13), D13 (= D15), G38 (= G41), G39 (≠ S42), K40 (≠ S43), N43 (≠ I46), E140 (≠ Q147), D252 (= D256)

Sites not aligning to the query:

4lc4A Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with guanosine
27% identity, 91% coverage: 1:285/314 of query aligns to 1:307/330 of 4lc4A

query
sites
4lc4A

M

M

G

T

K

R

R

F

D

D

L

V

L

L

V

T

V

V

G

G

E
x
N

L

A

N
x
I

I

V

D
|
D

-

I

-

S

-

R

-

C

-

N

-

D

-

Q

-

F

L

L

I

I

L

D

N

N

Q

Q

I

I

Q

T

G

K

-

A

-
x
M

-

N

-

L

F

I

P

D

E

A

M

E

G

R

S

A

E

E

K

L

V

L

A

Y

Q

S

K

R

M

M

D

G

V

P

V

A

L

L

-

E

-

A

-

S

G
|
G

S
x
G

S
|
S

S

A

A

G

I
x
N

F

T

A

A

N

G

I

V

A

A

T

N

L

L

G

G

V

G

D

K

T

A

A

A

F

Y

C

F

G

G

M

N

V

V

G

A

K

A

D

D

D

Q

F

L

G

G

K

D

M

I

V

F

Q

T

E

H

T

D

L

I

A

R

A

A

Q

Q

Q

G

V

V

D

H

T

Y

R

Q

F

T

I

K

A

P

T

K

S

G

A

A

Q

F