SitesBLAST
Comparing Echvi_0156 FitnessBrowser__Cola:Echvi_0156 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q53W83 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; EC 2.7.1.45 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
32% identity, 82% coverage: 26:284/314 of query aligns to 18:279/309 of Q53W83
- GAEVN 34:38 (≠ SSSAI 42:46) binding
- YYR 103:105 (≠ YC- 112:113) binding
- R167 (vs. gap) binding
- S193 (≠ N193) binding
- 219:225 (vs. 223:229, 71% identical) binding
- GAGD 248:251 (= GAGD 253:256) binding
- D251 (= D256) binding
- N275 (= N280) binding
Sites not aligning to the query:
1v1bA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound atp (see paper)
32% identity, 82% coverage: 26:284/314 of query aligns to 18:279/300 of 1v1bA
- active site: G248 (= G253), A249 (= A254), G250 (= G255), D251 (= D256)
- binding adenosine-5'-triphosphate: K219 (= K223), G221 (= G225), A238 (= A242), F239 (= F243), V241 (≠ N245), G248 (= G253), A249 (= A254), G250 (= G255), N275 (= N280), A279 (= A284)
1v1aA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound 2- keto-3-deoxygluconate and adp (see paper)
32% identity, 82% coverage: 26:284/314 of query aligns to 18:279/301 of 1v1aA
- active site: G248 (= G253), A249 (= A254), G250 (= G255), D251 (= D256)
- binding adenosine-5'-diphosphate: K219 (= K223), G221 (= G225), A222 (≠ V226), A249 (= A254), G250 (= G255), N275 (= N280), A279 (= A284)
- binding 2-keto-3-deoxygluconate: G34 (≠ S42), A35 (≠ S43), N38 (≠ I46), Y89 (≠ T98), R105 (vs. gap), R167 (vs. gap), G248 (= G253), D251 (= D256)
Sites not aligning to the query:
3in1A Crystal structure of a putative ribokinase in complex with adp from e.Coli
29% identity, 78% coverage: 48:293/314 of query aligns to 46:295/312 of 3in1A
- active site: R106 (= R107), G255 (= G253), A256 (= A254), G257 (= G255), D258 (= D256)
- binding adenosine-5'-diphosphate: N194 (= N193), K225 (= K223), G227 (= G225), G230 (= G229), A244 (= A242), T253 (≠ A251), N282 (= N280), A285 (≠ G283)
5eynA Crystal structure of fructokinase from vibrio cholerae o395 in fructose, adp, beryllium trifluoride and calcium ion bound form
29% identity, 84% coverage: 41:304/314 of query aligns to 27:303/306 of 5eynA
- active site: G246 (= G253), A247 (= A254), G248 (= G255), D249 (= D256)
- binding adenosine-5'-diphosphate: H91 (≠ D106), T217 (≠ K223), G219 (= G225), A220 (≠ V226), A238 (≠ N245), V239 (≠ D246), T244 (≠ A251), G246 (= G253), A247 (= A254), G248 (= G255), F251 (= F258), N279 (= N280), G282 (= G283), A283 (= A284)
- binding beryllium trifluoride ion: G246 (= G253), G248 (= G255), D249 (= D256)
- binding beta-D-fructofuranose: G28 (≠ S42), A29 (≠ S43), N32 (≠ I46), F96 (≠ V110), F98 (≠ Y112), R159 (≠ D171), D249 (= D256)
Sites not aligning to the query:
5yggA Crystal structure of fructokinase double-mutant (t261c-h108c) from vibrio cholerae o395 in fructose, adp and potassium ion bound form (see paper)
29% identity, 84% coverage: 41:304/314 of query aligns to 31:307/310 of 5yggA
- binding adenosine-5'-diphosphate: K188 (≠ L191), T221 (≠ K223), G223 (= G225), A242 (≠ N245), V243 (≠ D246), F255 (= F258), N283 (= N280), G286 (= G283), A287 (= A284)
- binding beta-D-fructofuranose: G32 (≠ S42), A33 (≠ S43), F100 (≠ V110), F102 (≠ Y112), R163 (≠ D171), D253 (= D256)
Sites not aligning to the query:
6ilsB Structure of arabidopsis thaliana ribokinase complexed with ribose and atp (see paper)
29% identity, 91% coverage: 6:291/314 of query aligns to 5:294/313 of 6ilsB
- binding adenosine-5'-triphosphate: N189 (= N193), K225 (= K223), G227 (= G225), I246 (≠ F243), A248 (≠ N245), A257 (= A254), G258 (= G255), F261 (= F258), A286 (≠ G283), S287 (≠ A284)
- binding alpha-D-ribofuranose: N12 (= N13), D14 (= D15), G40 (≠ S42), K41 (≠ S43), N44 (≠ I46), E144 (≠ G145), D259 (= D256)
A1A6H3 Ribokinase; AtRBSK; RK; EC 2.7.1.15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 95% coverage: 6:302/314 of query aligns to 71:371/379 of A1A6H3
Sites not aligning to the query:
- 1:74 mutation Missing: Loss of ribokinase activity.
4xckA Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
26% identity, 95% coverage: 6:304/314 of query aligns to 4:300/306 of 4xckA
- active site: A249 (≠ G253), A250 (= A254), G251 (= G255), D252 (= D256)
- binding adenosine-5'-diphosphate: T220 (≠ K223), G222 (= G225), S223 (≠ V226), V242 (≠ N245), T247 (≠ A251), A250 (= A254), F254 (= F258), H276 (≠ N280), A279 (≠ G283), V283 (≠ T287)
- binding alpha-D-ribofuranose: N11 (= N13), D13 (= D15), G39 (≠ S42), K40 (≠ S43), N43 (≠ I46), A95 (≠ T98), I107 (≠ V110), I109 (≠ G114), E140 (≠ Q147), T248 (≠ I252), D252 (= D256)
3iq0B Crystal structure of a putative ribokinase ii in complex with atp and mg+2 from e.Coli
28% identity, 79% coverage: 43:291/314 of query aligns to 37:290/308 of 3iq0B
- active site: G252 (= G253), A253 (= A254), G254 (= G255), D255 (= D256)
- binding adenosine-5'-triphosphate: S192 (≠ N193), K223 (= K223), G225 (= G225), E247 (≠ Q247), A253 (= A254), G254 (= G255), F257 (= F258), N279 (= N280), G282 (= G283)
8cqxA Ribokinase from t.Sp mutant a92g
28% identity, 91% coverage: 6:291/314 of query aligns to 2:283/300 of 8cqxA
- binding adenosine-5'-diphosphate: N179 (= N193), T217 (≠ K223), G219 (= G225), A220 (≠ V226), G222 (= G228), F250 (= F258), N272 (= N280), G275 (= G283), A276 (= A284), T279 (= T287)
- binding magnesium ion: D242 (= D250), T244 (≠ I252), A278 (≠ N286)
Sites not aligning to the query:
3uboA The crystal structure of adenosine kinase from sinorhizobium meliloti
27% identity, 78% coverage: 41:285/314 of query aligns to 57:306/338 of 3uboA
- active site: R124 (= R107), G274 (= G253), A275 (= A254), G276 (= G255), D277 (= D256)
- binding adenosine: G57 (= G41), G58 (≠ S42), S59 (= S43), N62 (≠ I46), M126 (≠ N109), T128 (= T111), E155 (≠ S138), Y157 (= Y140), G274 (= G253), D277 (= D256)
- binding adenosine-5'-diphosphate: N214 (= N193), T244 (≠ K223), S246 (≠ G225), E247 (≠ V226), G249 (= G228), L266 (≠ N245), A275 (= A254), G276 (= G255), N301 (= N280), A304 (≠ G283)
Sites not aligning to the query:
1gqtB Activation of ribokinase by monovalent cations (see paper)
26% identity, 84% coverage: 6:268/314 of query aligns to 6:266/307 of 1gqtB
- active site: A251 (≠ G253), A252 (= A254), G253 (= G255), D254 (= D256)
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N193), T222 (≠ K223), G224 (= G225), S225 (≠ V226), A252 (= A254), G253 (= G255)
- binding cesium ion: D248 (= D250), I250 (= I252)
- binding alpha-D-ribofuranose: N13 (= N13), D15 (= D15), G41 (≠ S42), N45 (≠ I46), E142 (≠ Q147), D254 (= D256)
Sites not aligning to the query:
P0A9J6 Ribokinase; RK; EC 2.7.1.15 from Escherichia coli (strain K12) (see 3 papers)
26% identity, 84% coverage: 6:268/314 of query aligns to 7:267/309 of P0A9J6
Sites not aligning to the query:
1rk2A E. Coli ribokinase complexed with ribose and adp, solved in space group p212121 (see paper)
26% identity, 84% coverage: 6:268/314 of query aligns to 4:264/305 of 1rk2A
- active site: A249 (≠ G253), A250 (= A254), G251 (= G255), D252 (= D256)
- binding adenosine-5'-diphosphate: T220 (≠ K223), G222 (= G225), S223 (≠ V226), A250 (= A254), G251 (= G255)
- binding tetrafluoroaluminate ion: G213 (≠ A217), R215 (≠ N218)
- binding magnesium ion: D246 (= D250)
- binding alpha-D-ribofuranose: N11 (= N13), D13 (= D15), G38 (= G41), G39 (≠ S42), K40 (≠ S43), N43 (≠ I46), E140 (≠ Q147), D252 (= D256)
Sites not aligning to the query:
4lc4A Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with guanosine
27% identity, 91% coverage: 1:285/314 of query aligns to 1:307/330 of 4lc4A
- active site: R125 (= R107), G275 (= G253), A276 (= A254), G277 (= G255), D278 (= D256)
- binding adenosine: N11 (≠ E11), I13 (≠ N13), D15 (= D15), M35 (vs. gap), G58 (= G41), G59 (≠ S42), S60 (= S43), N63 (≠ I46), M127 (≠ N109), T129 (= T111), E156 (≠ S138), Y158 (= Y140), T274 (≠ I252), D278 (= D256)
- binding guanosine: T245 (≠ K223), S247 (≠ G225), E248 (≠ V226), A305 (≠ G283)
Sites not aligning to the query:
4kbeA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with benzoguanamine
27% identity, 91% coverage: 1:285/314 of query aligns to 1:307/330 of 4kbeA
- active site: R125 (= R107), G275 (= G253), A276 (= A254), G277 (= G255), D278 (= D256)
- binding adenosine: N11 (≠ E11), I13 (≠ N13), D15 (= D15), M35 (vs. gap), G58 (= G41), G59 (≠ S42), S60 (= S43), N63 (≠ I46), M127 (≠ N109), T129 (= T111), E156 (≠ S138), Y158 (= Y140), D278 (= D256)
- binding 6-phenyl-1,3,5-triazine-2,4-diamine: I17 (vs. gap), S18 (vs. gap), R19 (vs. gap), P52 (≠ V38), A53 (≠ V39), L54 (= L40)
Sites not aligning to the query:
4kanA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with 2-(2,5-dimethyl-1,3-thiazol-4-yl)acetic acid
27% identity, 91% coverage: 1:285/314 of query aligns to 1:307/330 of 4kanA
- active site: R125 (= R107), G275 (= G253), A276 (= A254), G277 (= G255), D278 (= D256)
- binding adenosine: N11 (≠ E11), I13 (≠ N13), D15 (= D15), M35 (vs. gap), G58 (= G41), G59 (≠ S42), S60 (= S43), N63 (≠ I46), M127 (≠ N109), T129 (= T111), E156 (≠ S138), Y158 (= Y140), T274 (≠ I252), D278 (= D256)
- binding (2,5-dimethyl-1,3-thiazol-4-yl)acetic acid: I16 (vs. gap), A43 (≠ S29), E44 (= E30), Y47 (≠ A33), R114 (≠ L97), M116 (≠ V99), N215 (= N193), T245 (≠ K223), S247 (≠ G225), A276 (= A254)
Sites not aligning to the query:
4kalA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with quinoline-3-carboxylic acid
27% identity, 91% coverage: 1:285/314 of query aligns to 1:307/330 of 4kalA
- active site: R125 (= R107), G275 (= G253), A276 (= A254), G277 (= G255), D278 (= D256)
- binding adenosine: N11 (≠ E11), I13 (≠ N13), D15 (= D15), M35 (vs. gap), G58 (= G41), G59 (≠ S42), N63 (≠ I46), M127 (≠ N109), T129 (= T111), E156 (≠ S138), Y158 (= Y140), T274 (≠ I252), D278 (= D256)
- binding quinoline-3-carboxylic acid: S190 (≠ D167), F191 (≠ L168), D194 (= D171), S221 (= S199), Q224 (≠ G202)
4kahA Crystal structure of probable sugar kinase protein from rhizobium etli cfn 42 complexed with 4-bromo-1h-pyrazole
27% identity, 91% coverage: 1:285/314 of query aligns to 1:307/330 of 4kahA
- active site: R125 (= R107), G275 (= G253), A276 (= A254), G277 (= G255), D278 (= D256)
- binding adenosine: N11 (≠ E11), I13 (≠ N13), D15 (= D15), M35 (vs. gap), G58 (= G41), G59 (≠ S42), S60 (= S43), N63 (≠ I46), M127 (≠ N109), T129 (= T111), E156 (≠ S138), Y158 (= Y140), T274 (≠ I252), D278 (= D256)
- binding 4-bromo-1H-pyrazole: I16 (vs. gap), Y47 (≠ A33), L86 (≠ F69)
Sites not aligning to the query:
Query Sequence
>Echvi_0156 FitnessBrowser__Cola:Echvi_0156
MGKRDLLVVGELNIDLILNQIQGFPEMGSEKVAQKMDVVLGSSSAIFAANIATLGVDTAF
CGMVGKDDFGKMVQETLAAQQVDTRFIATSAQYKTGLTVVMNYDQDRANVTYCGAMEALT
MQEIPWEVAGTFKHFHLSNYFLQKGIQKDITAIFEKAKSAGMTTSLDLQVDPDDQWDFDY
ERCLPHVDIFLPNESELLSLTGKSSISEALEAIKPYANTVALKRGVKGGLVFEKGKVTEV
AAFVNDQFVDAIGAGDSFNAGFIHRFLQGADWESCLRFANLTGALNTTAAGGTGAFTSLA
AVKARAKSQFNQEI
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory