SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_0191 Echvi_0191 acetyl-CoA carboxylase, biotin carboxylase subunit to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4mv4A Crystal structure of biotin carboxylase from haemophilus influenzae in complex with amppcp and mg2 (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:438/442 of 4mv4A

query
sites
4mv4A

M

M

F

L

N

E

K

K

I

V

L

V

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

L

L

R

R

I

I

I

L

R

R

T

A

C

C

K

K

E

E

M

L

G

G

I

I

K

K

T

T

V

V

A

A

V

V

Y

H

S

S

T

T

A

A

D

D

K

R

D

D

S

L

L

K

H

H

V

V

R

L

F

L

A

A

D

D

E

E

A

T

V

I

C

C

I

I

G

G

A

P

A

A

P

P

S

S

R

A

E

K

S

S

Y

Y

L

L

N

N

I

I

P

P

R

A

V

I

I

I

A

A

E

E

I

V

T

T

N

G

A

A

D

D

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

S

S

E

E

N

N

A

A

E

D

F

F

S

A

K

E

I

Q

C

V

E

E

E

R

Y

S

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G

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F

F

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G

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S

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E

D

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M

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A

A

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T

A

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M

K

K

A

K

A

A

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G

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V

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P

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C

I

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P

G

G

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E

D

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-

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G

M

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E

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H

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D

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G

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K

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R

N

A

A

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L

E

S

E

E

F

T

V

I

I

I

D

D

G

G

I

I

K

K

T

T

T

N

I

I

P

P

F

L

H

H

I

E

A

L

L

I

L

L

E

E

D

D

E

E

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N

F

F

K

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E

active site: K116 (= K116), K159 (= K158), D193 (= D195), H206 (= H208), R232 (= R234), T271 (= T273), E273 (= E275), E285 (= E288), N287 (= N290), R289 (= R292), E293 (= E296), R335 (= R336)
binding phosphomethylphosphonic acid adenylate ester: I157 (= I156), K159 (= K158), G164 (= G163), M166 (= M168), E198 (= E200), K199 (= K201), Y200 (≠ F202), L201 (≠ V203), H233 (= H235), L275 (= L277), E285 (= E288)
binding magnesium ion: E273 (= E275), E285 (= E288), N287 (= N290)

6oi8A Crystal structure of haemophilus influenzae biotin carboxylase complexed with 7-((1r,5s,6s)-6-amino-3-azabicyclo[3.1.0]hexan-3-yl)- 6-(2-chloro-6-(pyridin-3-yl)phenyl)pyrido[2,3-d]pyrimidin-2-amine (see paper)
57% identity, 98% coverage: 1:440/449 of query aligns to 1:436/440 of 6oi8A

query
sites
6oi8A

M

M

F

L

N

E

K

K

I

V

L

V

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

L

L

R

R

I

I

I

L

R

R

T

A

C

C

K

K

E

E

M

L

G

G

I

I

K

K

T

T

V

V

A

A

V

V

Y

H

S

S

T

T

A

A

D

D

K

R

D

D

S

L

L

K

H

H

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V

R

L

F

L

A

A

D

D

E

E

A

T

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I

C

C

I

I

G

G

A

P

A

A

P

P

S

S

R

A

E

K

S

S

Y

Y

L

L

N

N

I

I

P

P

R

A

V

I

I

I

A

A

E

E

I

V

T

T

N

G

A

A

D

D

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

S

S

E

E

N

N

A

A

E

D

F

F

S

A

K

E

I

Q

C

V

E

E

E

R

Y

S

N

G

I

F

K

T

F

F

I

I

G

G

A

P

S

T

S

A

E

D

M

V

I

I

D

R

K

L

M

M

G

G

D

D

K
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K

A

V

T

S

A

A

K

I

A

K

T

A

M

M

K

K

A

K

A

A

G

G

V

V

P

P

T

C

I

V

P

P

G

G

S

S

E

D

G

G

-

P

L

V

L

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D

N

S

D

I

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A

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K

G

N

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K

K

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M

I

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G

Y

Y

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I
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I

L

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K

A

A

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S

A

G

G

-

R

-

G

G

M
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M

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R

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R

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E

E

K

D

E

A

F

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I

D

A

D

M

A

T

R

K

Q

A

E

E

S

A

G

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A

A

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F

G

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N

N

D
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D

G

M

L

V

Y

Y

L

M

E
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E

K
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K

F
x
Y

V
x
L

E

E

E

N

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H

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E

I

I

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Q

V

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G

A

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P

N

H

Y

Y

D

D

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S

M

M

I

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A

A

K

K

L

L

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G

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S

T

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R

E

E

E

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A

I

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R

R

R

M

M

K

Q

R

N

A

A

L

L

E

S

E

E

F

T

V

I

I

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D

D

G

G

I

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K

T

T

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N

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P

P

F

L

H

H

I

E

A

L

L

I

L

L

E

E

D

D

E

E

Q

N

F

F

K

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A

K

G

G

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G

F

T

T

N

T
x
I

K
x
H

F

Y

L

L

E

E

active site: K116 (= K116), K159 (= K158), D191 (= D195), H204 (= H208), R230 (= R234), T269 (= T273), E271 (= E275), E283 (= E288), N285 (= N290), R287 (= R292), E291 (= E296), R333 (= R336)
binding 7-[(1R,5S,6s)-6-amino-3-azabicyclo[3.1.0]hexan-3-yl]-6-[2-chloro-6-(pyridin-3-yl)phenyl]pyrido[2,3-d]pyrimidin-2-amine: I157 (= I156), K159 (= K158), M164 (= M168), E196 (= E200), K197 (= K201), Y198 (≠ F202), L199 (≠ V203), H204 (= H208), Q228 (= Q232), H231 (= H235), E271 (= E275), L273 (= L277), E283 (= E288), I432 (≠ T436), H433 (≠ K437)

4mv3A Crystal structure of biotin carboxylase from haemophilus influenzae in complex with amppcp and bicarbonate (see paper)
57% identity, 98% coverage: 1:440/449 of query aligns to 1:435/439 of 4mv3A

query
sites
4mv3A

M

M

F

L

N

E

K

K

I

V

L

V

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

L

L

R

R

I

I

I

L

R

R

T

A

C

C

K

K

E

E

M

L

G

G

I

I

K

K

T

T

V

V

A

A

V

V

Y

H

S

S

T

T

A

A

D

D

K

R

D

D

S

L

L

K

H

H

V

V

R

L

F

L

A

A

D

D

E

E

A

T

V

I

C

C

I

I

G

G

A

P

A

A

P

P

S

S

R

A

E

K

S

S

Y

Y

L

L

N

N

I

I

P

P

R

A

V

I

I

I

A

A

E

E

I

V

T

T

N

G

A

A

D

D

A

A

I

I

H

H

P

P

G

G

Y
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Y

G

G

F

F

L

L

S

S

E

E

N

N

A

A

E

D

F

F

S

A

K

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C

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E

E

E

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Y

S

N

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I

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K

T

F

F

I

I

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G

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E

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K

L

M

M

G

G

D

D

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K

A

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T

S

A

A

K

I

A

K

T

A

M

M

K

K

A

K

A

A

G

G

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C

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P

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G

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D

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K

E

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G

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Y

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I
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I

L

I

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K

A

A

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S

A

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G

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M
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M

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R

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D

A

D

M

A

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K

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A

E

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G

K

A

A

F

F

G

N

N

N

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G

M

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L

M

E
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E

K
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K

F
x
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x
L

E

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E

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H
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H

I

V

E

E

I

I

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Q

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D

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T

H

G

G

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N

A

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C

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A

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M

M

E

E

C

C

R
|
R

I

I

N

N

A

A

E

E

D

D

P

P

A

K

N

T

G

-

F

F

R

L

P

P

S

S

P

P

G

G

K

K

I

V

N

N

N

H

L

L

H

H

L

S

P

P

G

G

R

L

G

G

V

V

R

R

V

W

D

D

S

S

H

H

V

V

Y

Y

A

G

G

G

Y

Y

I

T

I

V

P

P

N

H

Y

Y

D

D

S

S

M

M

I

I

A

A

K

K

L

L

I

I

V

T

S

Y

G

G

Q

D

S

T

R

R

E

E

E

V

V

A

I

I

V

R

R

R

M

M

K

Q

R

N

A

A

L

L

E

S

E

E

F

T

V

I

I

I

D

D

G

G

I

I

K

K

T

T

T

N

I

I

P

P

F

L

H

H

I

E

A

L

L

I

L

L

E

E

D

D

E

E

Q

N

F

F

K

Q

A

K

G

G

N

G

F

T

T

N

T

I

K

H

F

Y

L

L

E

E

active site: K116 (= K116), K159 (= K158), D190 (= D195), H203 (= H208), R229 (= R234), T268 (= T273), E270 (= E275), E282 (= E288), N284 (= N290), R286 (= R292), E290 (= E296), R332 (= R336)
binding phosphomethylphosphonic acid adenylate ester: I157 (= I156), K159 (= K158), M163 (= M168), E195 (= E200), K196 (= K201), Y197 (≠ F202), L198 (≠ V203), H230 (= H235), E270 (= E275), L272 (= L277), I281 (≠ M287), E282 (= E288)
binding bicarbonate ion: Y82 (= Y82), K232 (= K237), R286 (= R292), Q288 (= Q294), V289 (= V295), E290 (= E296), R332 (= R336)

2vr1A Crystal structure of biotin carboxylase from e. Coli in complex with atp analog, adpcf2p. (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:439/444 of 2vr1A

query
sites
2vr1A

M

M

F

L

N

D

K

K

I

I

L

V

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

L

L

R

R

I

I

I

L

R

R

T

A

C

C

K

K

E

E

M

L

G

G

I

I

K

K

T

T

V

V

A

A

V

V

Y

H

S

S

T

S

A

A

D

D

K

R

D

D

S

L

L

K

H

H

V

V

R

L

F

L

A

A

D

D

E

E

A

T

V

V

C

C

I

I

G

G

A

P

A

A

P

P

S

S

R

V

E

K

S

S

Y

Y

L

L

N

N

I

I

P

P

R

A

V

I

I

I

A

S

A

A

E

E

I

I

T

T

N

G

A

A

D

V

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

S

S

E

E

N

N

A

A

E

N

F

F

S

A

K

E

I

Q

C

V

E

E

E

R

Y

S

N

G

I

F

K

I

F

F

I

I

G

G

A

P

S

K

S

A

E

E

M

T

I

I

D

R

K

L

M

M

G

G

D

D

K
|
K

A

V

T

S

A

A

K

I

A

A

T

A

M

M

K

K

A

K

A

A

G

G

V

V

P

P

T

C

I

V

P

P

G

G

S

S

E

D

G

G

L

P

L

L

-

G

D

D

S

D

I

M

E

D

Q

K

G

N

I

R

K

A

I

I

A

A

N

K

E

R

M

I

G

G

Y

Y

P

P

V

V

I
|
I

L

I

K
|
K

A

A

T

S

A

-

G

-

G

G

G
|
G

R
|
R

G

G

M
|
M

R

R

I

V

V

V

R

R

E

G

E

D

K

A

E

E

F

L

K

A

K

Q

A

S

W

I

D

S

D

M

A

T

R

R

Q

A

E

E

S

A

G

K

A

A

F

F

G

S

N

N

D
|
D

G

M

L

V

Y

Y

L

M

E
|
E

K
|
K

F
x
Y

V
x
L

E

E

E

N

P

P

R

R

H
|
H

I

V

E

E

I

I

Q

Q

V

V

V

L

G

A

D

D

N

G

T

Q

G

G

K

N

A

A

C

I

H

Y

L

L

S

A

E

E

R

R

D

D

C

C

S

S

I

M

Q
|
Q

R

R

R
|
R

H
|
H

Q

Q

K

K

L

V

V

V

E

E

T

A

P

P

S

A

P

P

F

G

I

I

T

T

D

P

E

E

L

L

R

R

D

R

A

Y

M

I

G

G

K

E

A

R

A

C

I

A

K

K

G

A

A

C

E

V

A

D

I

I

G

G

Y

Y

E

R

G

G

A

A

G

G

T
|
T

I

F

E
|
E

F

F

L
|
L

V

F

D

E

K

-

H

N

R

G

N

E

F

F

Y

Y

F

F

M

I

E
|
E

M

M

N
|
N

T

T

R
|
R

I

I

Q

Q

V

V

E
|
E

H

H

P

P

I

V

T

T

E

E

E

M

V

I

T

T

D

G

Y

V

D

D

L

L

I

I

K

K

E

E

Q

Q

I

L

K

R

V

I

A

A

G

G

I

Q

P

P

I

L

S

S

-

I

-

K

G

Q

Q

E

N

E

Y

V

Y

H

P

V

K

R

L

G

Y

H

A

A

M

V

E

E

C

C

R
|
R

I

I

N

N

A

A

E

E

D

D

P

P

A

-

N

N

G

T

F

F

R

L

P

P

S

S

P

P

G

G

K

K

I

I

N

T

N

R

L

F

H

H

L

A

P

P

G

G

R

F

G

G

V

V

R

R

V

W

D

E

S

S

H

H

V

I

Y

Y

A

A

G

G

Y

Y

I

T

I

V

P

P

N

Y

Y

Y

D

D

S

S

M

M

I

I

A

G

K

K

L

L

I

I

V

C

S

Y

G

G

Q

E

S

N

R

R

E

D

E

V

V

A

I

I

V

A

R

R

M

M

K

K

R

N

A

A

L

L

E

Q

E

E

F

L

V

I

I

I

D

D

G

G

I

I

K

K

T

T

T

N

I

V

P

D

F

L

H

Q

I

I

A

R

L

I

L

M

E

N

D

D

E

E

Q

N

F

F

K

Q

A

H

G

G

N

G

F

T

T

N

T
x
I

K

H

F

Y

L

L

E

E

active site: K116 (= K116), K159 (= K158), D194 (= D195), H207 (= H208), R233 (= R234), T272 (= T273), E274 (= E275), E286 (= E288), N288 (= N290), R290 (= R292), E294 (= E296), R336 (= R336)
binding phosphodifluoromethylphosphonic acid-adenylate ester: K116 (= K116), I157 (= I156), K159 (= K158), G164 (= G165), R165 (= R166), M167 (= M168), E199 (= E200), K200 (= K201), Y201 (≠ F202), L202 (≠ V203), Q231 (= Q232), H234 (= H235), E274 (= E275), L276 (= L277), E286 (= E288), N288 (= N290), I435 (≠ T436)

2vpqB Crystal structure of biotin carboxylase from s. Aureus complexed with amppnp (see paper)
57% identity, 97% coverage: 4:440/449 of query aligns to 2:441/448 of 2vpqB

query
sites
2vpqB

K

K

I

V

L

L

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

L

V

R

R

I

I

R

R

T

A

C

C

K

R

E

D

M

L

G

G

I

I

K

Q

T

T

V

V

A

A

V

I

Y

Y

S

S

T

E

A

G

D

D

K

K

D

D

S

A

L

L

H

H

V

T

R

Q

F

I

A

A

D

D

E

E

A

A

V

Y

C

C

I

V

G

G

A

P

A

T

P

L

S

S

R

K

E

D

S

S

Y

Y

L

L

N

N

I

I

P

P

R

N

V

I

I

L

A

S

A

I

A

A

E

T

I

S

T

T

N

G

A

C

D

D

A

G

I

V

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

S

A

E

E

N

N

A

A

E

D

F

F

S

A

K

E

I

L

C

C

E

E

E

A

Y

C

N

Q

I

L

K

K

F

F

I

I

G

G

A

P

S

S

S

Y

E

Q

M

S

I

I

D

Q

K

K

M

M

G

G

D

I

K
|
K

A

D

T
x
V

A

A

K

K

A

A

T

E

M

M

K

I

A

K

A

A

G

N

V

V

P

P

T

V

I

V

P

P

G

G

S

S

E

D

G

G

L

L

L

M

D

K

S

D

I

V

E

S

Q

E

G

A

I

K

K

K

I

I

A

A

N

K

E

K

M

I

G

G

Y

Y

P

P

V

V

I
|
I

L

I

K
|
K

A

A

T

T

A

A

G
|
G

R

K

G

G

M
x
I

R

R

I

V

V

A

R

R

E

D

E

E

K

K

E

E

F

L

K

E

K

T

A

G

W

F

D

R

D

M

A

T

R

E

Q

Q

E

E

S

A

G

Q

A

T

A

A

F

F

G

G

N

N

D

G

G

G

L

L

Y

Y

L

M

E
|
E

K
|
K

F
|
F

V
x
I

E

E

E

N

P
x
F

R

R

H
|
H

I

I

E

E

I

I

Q

Q

V

I

V

V

G

G

D

D

N

S

T

Y

G

G

K

N

A

V

C

I

H

H

L

L

S

G

E

E

R

R

D

D

C

C

S

T

I

I

Q
|
Q

R

R

R
|
R

H
x
M

Q

Q

K

K

L

L

V

V

E

E

T

A

P

P

S

S

P

P

F

I

I

L

T

D

D

D

E

E

L

T

R

R

D

R

A

E

M

M

G

G

K

N

A

A

I

V

K

R

G

A

A

A

E

K

A

A

I

V

G

N

Y

Y

E

E

G

N

A

A

G

G

T
|
T

I

I

E
|
E

F

F

L
x
I

V

Y

D

D

K

L

H

N

R

D

N

N

-

K

F

F

Y

Y

F

F

M
|
M

E
|
E

M

M

N
|
N

T

T

R
|
R

I

I

Q

Q

V

V

E
|
E

H

H

P

P

I

V

T

T

E

E

E

M

V

V

T

T

D

G

Y

I

D

D

L

L

I

V

K

K

E

L

Q

Q

I

L

K

Q

V

V

A

A

A

M

G

G

-

D

-

V

I

L

P

P

I

Y

S

K

G

Q

Q

E

N

D

Y

I

Y

K

P

L

K

T

L

G

Y

H

A

A

M

I

E

E

C

F

R
|
R

I

I

N

N

A

A

E

E

D

N

P

P

A

Y

N

K

G

N

F

F

R

M

P

P

S

S

P

P

G

G

K

K

I

I

N

E

N

Q

L

Y

H

L

L

A

P

P

G

G

R

Y

G

G

V

V

R

R

V

I

D

E

S

S

H

A

V

C

Y

Y

A

T

G

N

Y

Y

I

T

I

I

P

P

N

Y

Y

Y

D

D

S

S

M

M

I

V

A

A

K

K

L

L

I

I

V

I

S

H

G

E

Q

P

S

T

R

R

E

D

E

E

V

A

I

I

V

M

R

A

M

G

K

I

R

R

A

A

L

L

E

S

E

E

F

F

V

V

I

V

D

L

G

G

I

I

K

D

T

T

I

I

P

P

F

F

H

H

I

I

A

K

L

L

E

N

D

N

E

D

Q

I

F

F

K

R

A

S

G

G

N

K

F

F

T

N

T

T

K

N

F

F

L

L

E

E

active site: V116 (≠ T118), K156 (= K158), H206 (= H208), R232 (= R234), T271 (= T273), E273 (= E275), E287 (= E288), N289 (= N290), R291 (= R292), E295 (= E296), R337 (= R336)
binding phosphoaminophosphonic acid-adenylate ester: K114 (= K116), I154 (= I156), K156 (= K158), G160 (= G162), G161 (= G163), G162 (= G164), G163 (= G165), I166 (≠ M168), E198 (= E200), K199 (= K201), F200 (= F202), I201 (≠ V203), F204 (≠ P206), H206 (= H208), Q230 (= Q232), M233 (≠ H235), E273 (= E275), I275 (≠ L277), M286 (= M287), E287 (= E288), N289 (= N290), R291 (= R292)
binding magnesium ion: E273 (= E275), E287 (= E288)

P43873 Biotin carboxylase; Acetyl-coenzyme A carboxylase biotin carboxylase subunit A; EC 6.3.4.14 from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/448 of P43873

query
sites
P43873

M

M

F

L

N

E

K

K

I

V

L

V

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

L

L

R

R

I

I

I

L

R

R

T

A

C

C

K

K

E

E

M

L

G

G

I

I

K

K

T

T

V

V

A

A

V

V

Y

H

S

S

T

T

A

A

D

D

K

R

D

D

S

L

L

K

H

H

V

V

R

L

F

L

A

A

D

D

E

E

A

T

V

I

C

C

I

I

G

G

A

P

A

A

P

P

S

S

R

A

E

K

S

S

Y

Y

L

L

N

N

I

I

P

P

R

A

V

I

I

I

A

A

E

E

I

V

T

T

N

G

A

A

D

D

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

S

S

E

E

N

N

A

A

E

D

F

F

S

A

K

E

I

Q

C

V

E

E

E

R

Y

S

N

G

I

F

K

T

F

F

I

I

G

G

A

P

S

T

S

A

E

D

M

V

I

I

D

R

K

L

M

M

G

G

D

D

K
|
K

A

V

T

S

A

A

K

I

A

K

T

A

M

M

K

K

A

K

A

A

G

G

V

V

P

P

T

C

I

V

P

P

G

G

S

S

E

D

G

G

-

P

L

V

L

S

D

N

S

D

I

I

E

A

Q

K

G

N

I

K

K

E

I

I

A

A

N

K

E

R

M

I

G

G

Y

Y

P

P

V

I

I

I

L

I

K
|
K

A

A

T

S

A

G

G

G

R

R

G

G

M

M

R

R

I

V

V

V

R

R

E

S

E

E

K

D

E

A

F

L

K

E

A

S

W

I

D

A

D

M

A

T

R

K

Q

A

E

E

S

A

G

K

A

A

F

F

G

N

N

N

D

D

G

M

L

V

Y

Y

L

M

E
|
E

K
|
K

F
x
Y

V
x
L

E

E

E

N

P

P

R

R

H

H

I

V

E

E

I

I

Q

Q

V

V

V

L

G

A

D

D

N

T

T

H

G

G

K

N

A

A

C

V

H

Y

L

L

S

A

E

E

R

R

D

D

C

C

S

S

I

M

Q

Q

R

R

H

H

Q

Q

K

K

L

V

V

V

E

E

T

A

P

P

S

A

P

P

F

G

I

I

T

T

D

E

E

E

L

V

R

R

D

R

A

D

M

I

G

G

K

S

A

R

A

C

I

A

K

N

G

A

A

C

E

V

A

E

I

I

G

G

Y

Y

E

R

G

G

A

A

G

G

T

T

I

F

E
|
E

F

F

L

L

V

Y

D

E

K

-

H

N

R

G

N

E

F

F

Y

Y

F

F

M

I

E
|
E

M

M

N
|
N

T

T

R

R

I

I

Q

Q

V

V

E

E

H

H

P

P

I

V

T

T

E

E

E

M

V

I

T

T

D

G

Y

V

D

D

L

L

I

V

K

K

E

E

Q

Q

I

L

K

R

V

I

A

A

G

G

I

L

P

P

I

I

S

S

-

F

-

K

G

Q

Q

E

N

D

Y

I

Y

K

P

V

K

K

L

G

Y

H

A

A

M

M

E

E

C

C

R

R

I

I

N

N

A

A

E

E

D

D

P

P

A

K

N

T

G

-

F

F

R

L

P

P

S

S

P

P

G

G

K

K

I

V

N

N

N

H

L

L

H

H

L

S

P

P

G

G

R

L

G

G

V

V

R

R

V

W

D

D

S

S

H

H

V

V

Y

Y

A

G

G

G

Y

Y

I

T

I

V

P

P

N

H

Y

Y

D

D

S

S

M

M

I

I

A

A

K

K

L

L

I

I

V

T

S

Y

G

G

Q

D

S

T

R

R

E

E

E

V

V

A

I

I

V

R

R

R

M

M

K

Q

R

N

A

A

L

L

E

S

E

E

F

T

V

I

I

I

D

D

G

G

I

I

K

K

T

T

T

N

I

I

P

P

F

L

H

H

I

E

A

L

L

I

L

L

E

E

D

D

E

E

Q

N

F

F

K

Q

A

K

G

G

N

G

F

T

T

N

T

I

K

H

F

Y

L

L

E

E

K116 (= K116) binding
K159 (= K158) binding
EKYL 201:204 (≠ EKFV 200:203) binding
E276 (= E275) binding ; binding
E288 (= E288) binding ; binding
N290 (= N290) binding

4mv1A Crystal structure of biotin carboxylase from haemophilus influenzae in complex with adp and phosphate (see paper)
57% identity, 98% coverage: 1:440/449 of query aligns to 1:427/430 of 4mv1A

query
sites
4mv1A

M

M

F

L

N

E

K

K

I

V

L

V

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

L

L

R

R

I

I

I

L

R

R

T

A

C

C

K

K

E

E

M

L

G

G

I

I

K

K

T

T

V

V

A

A

V

V

Y

H

S

S

T

T

A

A

D

D

K

R

D

D

S

L

L

K

H

H

V

V

R

L

F

L

A

A

D

D

E

E

A

T

V

I

C

C

I

I

G

G

A

P

A

A

P

P

S

S

R

A

E

K

S

S

Y

Y

L

L

N

N

I

I

P

P

R

A

V

I

I

I

A

A

E

E

I

V

T

T

N

G

A

A

D

D

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

S

S

E

E

N

N

A

A

E

D

F

F

S

A

K

E

I

Q

C

V

E

E

E

R

Y

S

N

G

I

F

K

T

F

F

I

I

G

G

A

P

S

T

S

A

E

D

M

V

I

I

D

R

K

L

M

M

G

G

D

D

K
|
K

A

V

T

S

A

A

K

I

A

K

T

A

M

M

K

K

A

K

A

A

G

G

V

V

P

P

T

C

I
x
V

P

P

G

G

S

S

E

D

G

G

-

P

L

V

L

S

D

N

S

D

I

I

E

A

Q

K

G

N

I

K

K

E

I

I

A

A

N

K

E

R

M

I

G

G

Y

Y

P

P

V

I

I

I

L

I

K
|
K

A

A

T

S

A

-

G

-

R

-

G

G

M
|
M

R

R

I

V

V

V

R

R

E

S

E

E

K

-

E

-

F

-

K

-

A

-

W

-

D

-

D

D

A

A

R

L

Q

E

E

E

S

S

G

I

A

A

A

M

F

T

G

K

N

A

D
|
D

G

M

L

V

Y

Y

L

M

E
|
E

K
|
K

F
x
Y

V
x
L

E

E

E

N

P

P

R

R

H
|
H

I

V

E

E

I

I

Q

Q

V

V

V

L

G

A

D

D

N

T

T

H

G

G

K

N

A

A

C

V

H

Y

L

L

S

A

E

E

R

R

D

D

C

C

S

S

I

M

Q

Q

R

R

R
|
R

H
|
H

Q

Q

K
|
K

L

V

V

V

E

E

T

A

P

P

S

A

P

P

F

G

I

I

T

T

D

E

E

E

L

V

R

R

D

R

A

D

M

I

G

G

K

S

A

R

A

C

I

A

K

N

G

A

A

C

E

V

A

E

I

I

G

G

Y

Y

E

R

G

G

A

A

G

G

T
|
T

I

F

E
|
E

F

F

L
|
L

V

Y

D

E

K

-

H

N

R

G

N

E

F

F

Y

Y

F

F

M

I

E
|
E

M

M

N
|
N

T

T

R
|
R

I

I

Q
|
Q

V
|
V

E
|
E

H

H

P

P

I

V

T

T

E

E

E

M

V

I

T

T

D

G

Y

V

D

D

L

L

I

V

K

K

E

E

Q

Q

I

L

K

R

V

I

A

A

G

G

I

L

P

P

I

I

S

S

-

F

-

K

G

Q

Q

E

N

D

Y

I

Y

K

P

V

K

K

L

G

Y

H

A

A

M

M

E

E

C

C

R
|
R

I

I

N

N

A

A

E

E

D

D

P

P

A

K

N

T

G

-

F

F

R

L

P

P

S

S

P

P

G

G

K

K

I

V

N

N

N

H

L

L

H

H

L

S

P

P

G

G

R

L

G

G

V

V

R

R

V

W

D

D

S

S

H

H

V

V

Y

Y

A

G

G

G

Y

Y

I

T

I

V

P

P

N

H

Y

Y

D

D

S

S

M

M

I

I

A

A

K

K

L

L

I

I

V

T

S

Y

G

G

Q

D

S

T

R

R

E

E

E

V

V

A

I

I

V

R

R

R

M

M

K

Q

R

N

A

A

L

L

E

S

E

E

F

T

V

I

I

I

D

D

G

G

I

I

K

K

T

T

T

N

I

I

P

P

F

L

H

H

I

E

A

L

L

I

L

L

E

E

D

D

E

E

Q

N

F

F

K

Q

A

K

G

G

N

G

F

T

T

N

T

I

K

H

F

Y

L

L

E

E

active site: K116 (= K116), K159 (= K158), D182 (= D195), H195 (= H208), R221 (= R234), T260 (= T273), E262 (= E275), E274 (= E288), N276 (= N290), R278 (= R292), E282 (= E296), R324 (= R336)
binding adenosine-5'-diphosphate: K116 (= K116), V131 (≠ I131), K159 (= K158), M163 (= M168), E187 (= E200), K188 (= K201), Y189 (≠ F202), L190 (≠ V203), H222 (= H235), L264 (= L277), E274 (= E288)
binding phosphate ion: K224 (= K237), R278 (= R292), Q280 (= Q294), V281 (= V295), E282 (= E296), R324 (= R336)

6ojhA Crystal structure of haemophilus influenzae biotin carboxylase complexed with (r)-7-(3-aminopyrrolidin-1-yl)-6-(naphthalen-1-yl) pyrido[2,3-d]pyrimidin-2-amine
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/445 of 6ojhA

query
sites
6ojhA

M

M

F

L

N

E

K

K

I

V

L

V

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

L

L

R

R

I

I

I

L

R

R

T

A

C

C

K

K

E

E

M

L

G

G

I

I

K

K

T

T

V

V

A

A

V

V

Y

H

S

S

T

T

A

A

D

D

K

R

D

D

S

L

L

K

H

H

V

V

R

L

F

L

A

A

D

D

E

E

A

T

V

I

C

C

I

I

G

G

A

P

A

A

P

P

S

S

R

A

E

K

S

S

Y

Y

L

L

N

N

I

I

P

P

R

A

V

I

I

I

A

A

E

E

I

V

T

T

N

G

A

A

D

D

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

S

S

E

E

N

N

A

A

E

D

F

F

S

A

K

E

I

Q

C

V

E

E

E

R

Y

S

N

G

I

F

K

T

F

F

I

I

G

G

A

P

S

T

S

A

E

D

M

V

I

I

D

R

K

L

M

M

G

G

D

D

K
|
K

A

V

T

S

A

A

K

I

A

K

T

A

M

M

K

K

A

K

A

A

G

G

V

V

P

P

T

C

I
x
V

P

P

G

G

S

S

E

D

G

G

-

P

L

V

L

S

D

N

S

D

I

I

E

A

Q

K

G

N

I

K

K

E

I

I

A

A

N

K

E

R

M

I

G

G

Y

Y

P

P

V

I

I
|
I

L

I

K
|
K

A

A

T

S

A

G

G

G

G
|
G

R

R

G

G

M
|
M

R

R

I

V

V

V

R

R

E

S

E

E

K

D

E

A

F

L

K

E

A

S

W

I

D

A

D

M

A

T

R

K

Q

A

E

E

S

A

G

K

A

A

F

F

G

N

N

N

D
|
D

G

M

L

V

Y

Y

L

M

E
|
E

K
|
K

F
x
Y

V
x
L

E

E

E

N

P

P

R

R

H
|
H

I

V

E

E

I

I

Q

Q

V

V

V

L

G

A

D

D

N

T

T

H

G

G

K

N

A

A

C

V

H

Y

L

L

S

A

E

E

R

R

D

D

C

C

S

S

I

M

Q
|
Q

R

R

R
|
R

H
|
H

Q

Q

K

K

L

V

V

V

E

E

T

A

P

P

S

A

P

P

F

G

I

I

T

T

D

E

E

E

L

V

R

R

D

R

A

D

M

I

G

G

K

S

A

R

A

C

I

A

K

N

G

A

A

C

E

V

A

E

I

I

G

G

Y

Y

E

R

G

G

A

A

G

G

T
|
T

I

F

E
|
E

F

F

L
|
L

V

Y

D

E

K

-

H

N

R

G

N

E

F

F

Y

Y

F

F

M

I

E
|
E

M

M

N
|
N

T

T

R
|
R

I

I

Q

Q

V

V

E
|
E

H

H

P

P

I

V

T

T

E

E

E

M

V

I

T

T

D

G

Y

V

D

D

L

L

I

V

K

K

E

E

Q

Q

I

L

K

R

V

I

A

A

G

G

I

L

P

P

I

I

S

S

-

F

-

K

G

Q

Q

E

N

D

Y

I

Y

K

P

V

K

K

L

G

Y

H

A

A

M

M

E

E

C

C

R
|
R

I

I

N

N

A

A

E

E

D

D

P

P

A

K

N

T

G

-

F

F

R

L

P

P

S

S

P

P

G

G

K

K

I

V

N

N

N

H

L

L

H

H

L

S

P

P

G

G

R

L

G

G

V

V

R

R

V

W

D

D

S

S

H

H

V

V

Y

Y

A

G

G

G

Y

Y

I

T

I

V

P

P

N

H

Y

Y

D

D

S

S

M

M

I

I

A

A

K

K

L

L

I

I

V

T

S

Y

G

G

Q

D

S

T

R

R

E

E

E

V

V

A

I

I

V

R

R

R

M

M

K

Q

R

N

A

A

L

L

E

S

E

E

F

T

V

I

I

I

D

D

G

G

I

I

K

K

T

T

T

N

I

I

P

P

F

L

H

H

I

E

A

L

L

I

L

L

E

E

D

D

E

E

Q

N

F

F

K

Q

A

K

G

G

N

G

F

T

T

N

T
x
I

K

H

F

Y

L

L

E

E

active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E288), N290 (= N290), R292 (= R292), E296 (= E296), R338 (= R336)
binding calcium ion: E276 (= E275), E288 (= E288), N290 (= N290)
binding 7-[(3R)-3-aminopyrrolidin-1-yl]-6-(naphthalen-1-yl)pyrido[2,3-d]pyrimidin-2-amine: V131 (≠ I131), I157 (= I156), K159 (= K158), G166 (= G165), M169 (= M168), E201 (= E200), K202 (= K201), Y203 (≠ F202), L204 (≠ V203), H209 (= H208), Q233 (= Q232), H236 (= H235), L278 (= L277), E288 (= E288), I437 (≠ T436)

3rupA Crystal structure of e.Coli biotin carboxylase in complex with two adp and two ca ions (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/444 of 3rupA

query
sites
3rupA

M

M

F

L

N

D

K

K

I

I

L

V

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

L

L

R

R

I

I

I

L

R

R

T

A

C

C

K

K

E

E

M

L

G

G

I

I

K

K

T

T

V

V

A

A

V

V

Y

H

S

S

T

S

A

A

D

D

K

R

D

D

S

L

L

K

H

H

V

V

R

L

F

L

A

A

D

D

E

E

A

T

V

V

C

C

I

I

G

G

A

P

A

A

P

P

S

S

R

V

E

K

S

S

Y

Y

L

L

N

N

I

I

P

P

R

A

V

I

I

I

A

S

A

A

E

E

I

I

T

T

N

G

A

A

D

V

A

A

I

I

H

H

P

P

G

G

Y
|
Y

G
|
G

F

F

L

L

S

S

E
|
E

N

N

A

A

E

N

F

F

S

A

K

E

I

Q

C

V

E

E

E

R

Y

S

N

G

I

F

K

I

F

F

I

I

G

G

A

P

S

K

S

A

E

E

M

T

I

I

D

R

K

L

M

M

G

G

D

D

K
|
K

A

V

T

S

A

A

K

I

A

A

T

A

M

M

K

K

A

K

A

A

G

G

V

V

P

P

T

C

I

V

P

P

G

G

S

S

E

D

G

G

L

P

L

L

-

G

D

D

S

D

I

M

E

D

Q

K

G

N

I

R

K

A

I

I

A

A

N

K

E

R

M

I

G

G

Y

Y

P

P

V

V

I
|
I

L

I

K
|
K

A

A

T

S

A
x
G

G
|
G

R
|
R

G

G

M
|
M

R

R

I

V

V

V

R

R

E

G

E

D

K

A

E

E

F

L

K

A

K

Q

A

S

W

I

D

S

D

M

A

T

R

R

Q

A

E

E

S

A

G

K

A

A

F
|
F

G

S

N

N

D
|
D

G

M

L

V

Y

Y

L

M

E
|
E

K
|
K

F
x
Y

V
x
L

E

E

E

N

P

P

R

R

H
|
H

I

V

E

E

I

I

Q

Q

V

V

V

L

G

A

D

D

N

G

T

Q

G

G

K

N

A

A

C

I

H

Y

L

L

S

A

E

E

R

R

D

D

C

C

S

S

I

M

Q
|
Q

R

R

R
|
R

H
|
H

Q
|
Q

K
|
K

L

V

V

V

E

E

T

A

P

P

S

A

P

P

F

G

I

I

T

T

D

P

E

E

L

L

R

R

D

R

A

Y

M

I

G

G

K

E

A

R

A

C

I

A

K

K

G

A

A

C

E

V

A

D

I

I

G

G

Y

Y

E

R

G

G

A

A

G

G

T
|
T

I

F

E
|
E

F

F

L
|
L

V

F

D

E

K

-

H

N

R

G

N

E

F

F

Y

Y

F

F

M
x
I

E
|
E

M

M

N
|
N

T

T

R
|
R

I

I

Q
|
Q

V
|
V

E
|
E

H

H

P

P

I

V

T

T

E

E

E

M

V

I

T

T

D

G

Y

V

D

D

L

L

I

I

K

K

E

E

Q

Q

I

L

K

R

V

I

A

A

G

G

I

Q

P

P

I

L

S

S

-

I

-

K

G

Q

Q

E

N

E

Y

V

Y

H

P

V

K

R

L

G

Y

H

A

A

M

V

E

E

C

C

R
|
R

I

I

N

N

A

A

E

E

D

D

P

P

A

-

N

N

G

T

F

F

R

L

P

P

S

S

P

P

G

G

K

K

I

I

N

T

N

R

L

F

H

H

L

A

P

P

G

G

R

F

G

G

V

V

R

R

V

W

D

E

S

S

H

H

V

I

Y

Y

A

A

G

G

Y

Y

I

T

I

V

P

P

N

Y

Y

Y

D
|
D

S

S

M

M

I

I

A

G

K

K

L

L

I

I

V

C

S

Y

G

G

Q

E

S

N

R

R

E

D

E

V

V

A

I

I

V

A

R

R

M

M

K

K

R

N

A

A

L

L

E

Q

E

E

F

L

V

I

I

I

D

D

G

G

I

I

K

K

T

T

T

N

I

V

P

D

F

L

H

Q

I

I

A

R

L

I

L

M

E

N

D

D

E

E

Q

N

F

F

K

Q

A

H

G

G

N

G

F

T

T

N

T
x
I

K

H

F

Y

L

L

E

E

active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E288), N290 (= N290), R292 (= R292), E296 (= E296), R338 (= R336)
binding adenosine-5'-diphosphate: Y82 (= Y82), G83 (= G83), E87 (= E87), K116 (= K116), I157 (= I156), K159 (= K158), G162 (≠ A161), G163 (= G162), G164 (= G163), G164 (= G163), G165 (= G164), G165 (= G164), G166 (= G165), R167 (= R166), M169 (= M168), F193 (= F192), E201 (= E200), K202 (= K201), Y203 (≠ F202), L204 (≠ V203), H209 (= H208), Q233 (= Q232), H236 (= H235), Q237 (= Q236), K238 (= K237), L278 (= L277), I287 (≠ M287), E288 (= E288), N290 (= N290), R292 (= R292), Q294 (= Q294), V295 (= V295), E296 (= E296), R338 (= R336), D382 (= D381), I437 (≠ T436)
binding calcium ion: E87 (= E87), E276 (= E275), E288 (= E288), E288 (= E288), N290 (= N290)

3g8cA Crystal structure of biotin carboxylase in complex with biotin, bicarbonate, adp and mg ion (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/444 of 3g8cA

query
sites
3g8cA

M

M

F

L

N

D

K

K

I

I

L

V

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

L

L

R

R

I

I

I

L

R

R

T

A

C

C

K

K

E

E

M

L

G

G

I

I

K

K

T

T

V

V

A

A

V

V

Y

H

S

S

T

S

A

A

D

D

K

R

D

D

S

L

L

K

H

H

V

V

R

L

F

L

A

A

D

D

E

E

A

T

V

V

C

C

I

I

G

G

A

P

A

A

P

P

S

S

R

V

E

K

S

S

Y

Y

L

L

N

N

I

I

P

P

R

A

V

I

I

I

A

S

A

A

E

E

I

I

T

T

N

G

A

A

D

V

A

A

I

I

H

H

P

P

G

G

Y
|
Y

G
|
G

F
|
F

L

L

S

S

E

E

N

N

A

A

E

N

F

F

S

A

K

E

I

Q

C

V

E

E

E

R

Y

S

N

G

I

F

K

I

F

F

I

I

G

G

A

P

S

K

S

A

E

E

M

T

I

I

D

R

K

L

M

M

G

G

D

D

K
|
K

A

V

T

S

A

A

K

I

A

A

T

A

M

M

K

K

A

K

A

A

G

G

V

V

P

P

T

C

I

V

P

P

G

G

S

S

E

D

G

G

L

P

L

L

-

G

D

D

S

D

I

M

E

D

Q

K

G

N

I

R

K

A

I

I

A

A

N

K

E

R

M

I

G

G

Y

Y

P

P

V

V

I
|
I

L

I

K
|
K

A

A

T

S

A

G

G
|
G

G

G

R

R

G

G

M
|
M

R

R

I

V

V

V

R

R

E

G

E

D

K

A

E

E

F

L

K

A

K

Q

A

S

W

I

D

S

D

M

A

T

R

R

Q

A

E

E

S

A

G

K

A

A

F

F

G

S

N

N

D
|
D

G

M

L

V

Y

Y

L

M

E
|
E

K
|
K

F
x
Y

V
x
L

E

E

E

N

P

P

R

R

H
|
H

I

V

E

E

I

I

Q

Q

V

V

V

L

G

A

D

D

N

G

T

Q

G

G

K

N

A

A

C

I