SitesBLAST
Comparing Echvi_0191 Echvi_0191 acetyl-CoA carboxylase, biotin carboxylase subunit to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4mv4A Crystal structure of biotin carboxylase from haemophilus influenzae in complex with amppcp and mg2 (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:438/442 of 4mv4A
- active site: K116 (= K116), K159 (= K158), D193 (= D195), H206 (= H208), R232 (= R234), T271 (= T273), E273 (= E275), E285 (= E288), N287 (= N290), R289 (= R292), E293 (= E296), R335 (= R336)
- binding phosphomethylphosphonic acid adenylate ester: K159 (= K158), G164 (= G163), M166 (= M168), E198 (= E200), Y200 (≠ F202), L201 (≠ V203), H233 (= H235), L275 (= L277), E285 (= E288)
- binding magnesium ion: E273 (= E275), E285 (= E288)
6oi8A Crystal structure of haemophilus influenzae biotin carboxylase complexed with 7-((1r,5s,6s)-6-amino-3-azabicyclo[3.1.0]hexan-3-yl)- 6-(2-chloro-6-(pyridin-3-yl)phenyl)pyrido[2,3-d]pyrimidin-2-amine (see paper)
57% identity, 98% coverage: 1:440/449 of query aligns to 1:436/440 of 6oi8A
- active site: K116 (= K116), K159 (= K158), D191 (= D195), H204 (= H208), R230 (= R234), T269 (= T273), E271 (= E275), E283 (= E288), N285 (= N290), R287 (= R292), E291 (= E296), R333 (= R336)
- binding 7-[(1R,5S,6s)-6-amino-3-azabicyclo[3.1.0]hexan-3-yl]-6-[2-chloro-6-(pyridin-3-yl)phenyl]pyrido[2,3-d]pyrimidin-2-amine: I157 (= I156), K159 (= K158), M164 (= M168), E196 (= E200), Y198 (≠ F202), L199 (≠ V203), H204 (= H208), Q228 (= Q232), E271 (= E275), L273 (= L277), E283 (= E288), I432 (≠ T436)
4mv3A Crystal structure of biotin carboxylase from haemophilus influenzae in complex with amppcp and bicarbonate (see paper)
57% identity, 98% coverage: 1:440/449 of query aligns to 1:435/439 of 4mv3A
- active site: K116 (= K116), K159 (= K158), D190 (= D195), H203 (= H208), R229 (= R234), T268 (= T273), E270 (= E275), E282 (= E288), N284 (= N290), R286 (= R292), E290 (= E296), R332 (= R336)
- binding phosphomethylphosphonic acid adenylate ester: K159 (= K158), M163 (= M168), E195 (= E200), Y197 (≠ F202), L198 (≠ V203), E270 (= E275), L272 (= L277), E282 (= E288)
- binding bicarbonate ion: R286 (= R292), Q288 (= Q294), V289 (= V295)
2vr1A Crystal structure of biotin carboxylase from e. Coli in complex with atp analog, adpcf2p. (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:439/444 of 2vr1A
- active site: K116 (= K116), K159 (= K158), D194 (= D195), H207 (= H208), R233 (= R234), T272 (= T273), E274 (= E275), E286 (= E288), N288 (= N290), R290 (= R292), E294 (= E296), R336 (= R336)
- binding phosphodifluoromethylphosphonic acid-adenylate ester: K159 (= K158), R165 (= R166), M167 (= M168), Y201 (≠ F202), L202 (≠ V203), E274 (= E275), L276 (= L277), E286 (= E288), N288 (= N290), I435 (≠ T436)
2vpqB Crystal structure of biotin carboxylase from s. Aureus complexed with amppnp (see paper)
57% identity, 97% coverage: 4:440/449 of query aligns to 2:441/448 of 2vpqB
- active site: V116 (≠ T118), K156 (= K158), H206 (= H208), R232 (= R234), T271 (= T273), E273 (= E275), E287 (= E288), N289 (= N290), R291 (= R292), E295 (= E296), R337 (= R336)
- binding phosphoaminophosphonic acid-adenylate ester: K114 (= K116), I154 (= I156), K156 (= K158), G161 (= G163), G163 (= G165), I166 (≠ M168), F200 (= F202), I201 (≠ V203), E273 (= E275), I275 (≠ L277), M286 (= M287), E287 (= E288)
- binding magnesium ion: E273 (= E275), E287 (= E288)
P43873 Biotin carboxylase; Acetyl-coenzyme A carboxylase biotin carboxylase subunit A; EC 6.3.4.14 from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/448 of P43873
- K116 (= K116) binding
- K159 (= K158) binding
- EKYL 201:204 (≠ EKFV 200:203) binding
- E276 (= E275) binding ; binding
- E288 (= E288) binding ; binding
- N290 (= N290) binding
4mv1A Crystal structure of biotin carboxylase from haemophilus influenzae in complex with adp and phosphate (see paper)
57% identity, 98% coverage: 1:440/449 of query aligns to 1:427/430 of 4mv1A
- active site: K116 (= K116), K159 (= K158), D182 (= D195), H195 (= H208), R221 (= R234), T260 (= T273), E262 (= E275), E274 (= E288), N276 (= N290), R278 (= R292), E282 (= E296), R324 (= R336)
- binding adenosine-5'-diphosphate: K159 (= K158), E187 (= E200), K188 (= K201), Y189 (≠ F202), L190 (≠ V203), L264 (= L277)
- binding phosphate ion: K224 (= K237), R278 (= R292), Q280 (= Q294), V281 (= V295), E282 (= E296)
6ojhA Crystal structure of haemophilus influenzae biotin carboxylase complexed with (r)-7-(3-aminopyrrolidin-1-yl)-6-(naphthalen-1-yl) pyrido[2,3-d]pyrimidin-2-amine
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/445 of 6ojhA
- active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E288), N290 (= N290), R292 (= R292), E296 (= E296), R338 (= R336)
- binding calcium ion: E276 (= E275), E288 (= E288), N290 (= N290)
- binding 7-[(3R)-3-aminopyrrolidin-1-yl]-6-(naphthalen-1-yl)pyrido[2,3-d]pyrimidin-2-amine: K159 (= K158), M169 (= M168), E201 (= E200), Y203 (≠ F202), L204 (≠ V203), H236 (= H235), L278 (= L277), E288 (= E288), I437 (≠ T436)
3rupA Crystal structure of e.Coli biotin carboxylase in complex with two adp and two ca ions (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/444 of 3rupA
- active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E288), N290 (= N290), R292 (= R292), E296 (= E296), R338 (= R336)
- binding adenosine-5'-diphosphate: Y82 (= Y82), G83 (= G83), K116 (= K116), K159 (= K158), G164 (= G163), G164 (= G163), G165 (= G164), G166 (= G165), R167 (= R166), M169 (= M168), F193 (= F192), E201 (= E200), K202 (= K201), Y203 (≠ F202), L204 (≠ V203), H209 (= H208), Q233 (= Q232), H236 (= H235), K238 (= K237), L278 (= L277), E288 (= E288), R292 (= R292), V295 (= V295), E296 (= E296), R338 (= R336), D382 (= D381), I437 (≠ T436)
- binding calcium ion: E87 (= E87), E276 (= E275), E288 (= E288), E288 (= E288), N290 (= N290)
3g8cA Crystal structure of biotin carboxylase in complex with biotin, bicarbonate, adp and mg ion (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/444 of 3g8cA
- active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E288), N290 (= N290), R292 (= R292), E296 (= E296), R338 (= R336)
- binding adenosine-5'-diphosphate: I157 (= I156), K159 (= K158), G164 (= G163), M169 (= M168), E201 (= E200), K202 (= K201), Y203 (≠ F202), L204 (≠ V203), Q233 (= Q232), H236 (= H235), L278 (= L277), E288 (= E288), I437 (≠ T436)
- binding bicarbonate ion: K238 (= K237), R292 (= R292), Q294 (= Q294), V295 (= V295), E296 (= E296)
- binding biotin: Y82 (= Y82), F84 (= F84), R292 (= R292), V295 (= V295), R338 (= R336), D382 (= D381)
- binding magnesium ion: E276 (= E275), E288 (= E288)
6oi9A Crystal structure of e. Coli biotin carboxylase complexed with 7-[3- (aminomethyl)pyrrolidin-1-yl]-6-(2,6-dichlorophenyl)pyrido[2,3- d]pyrimidin-2-amine (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/446 of 6oi9A
- active site: E276 (= E275), E288 (= E288), N290 (= N290), E296 (= E296), R338 (= R336)
- binding 7-[(3S)-3-(aminomethyl)pyrrolidin-1-yl]-6-(2,6-dichlorophenyl)pyrido[2,3-d]pyrimidin-2-amine: K159 (= K158), M169 (= M168), E201 (= E200), Y203 (≠ F202), L204 (≠ V203), H209 (= H208), Q233 (= Q232), H236 (= H235), E276 (= E275), L278 (= L277), E288 (= E288), I437 (≠ T436)
2w71A Crystal structure of biotin carboxylase from e. Coli in complex with the imidazole-pyrimidine inhibitor (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/446 of 2w71A
- active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E288), N290 (= N290), R292 (= R292), E296 (= E296), R338 (= R336)
- binding 4-[1-(2,6-dichlorobenzyl)-2-methyl-1H-imidazol-4-yl]pyrimidin-2-amine: K159 (= K158), Y203 (≠ F202), L204 (≠ V203), H209 (= H208), Q233 (= Q232), H236 (= H235), L278 (= L277), I437 (≠ T436)
2w70A Crystal structure of biotin carboxylase from e. Coli in complex with the amino-thiazole-pyrimidine fragment (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/446 of 2w70A
- active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E288), N290 (= N290), R292 (= R292), E296 (= E296), R338 (= R336)
- binding 4-(2-amino-1,3-thiazol-4-yl)pyrimidin-2-amine: I157 (= I156), K159 (= K158), G166 (= G165), M169 (= M168), E201 (= E200), Y203 (≠ F202), L204 (≠ V203), L278 (= L277)
2w6zA Crystal structure of biotin carboxylase from e. Coli in complex with the 3-(3-methyl-but-2-enyl)-3h-purin-6-ylamine fragment (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/446 of 2w6zA
- active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E288), N290 (= N290), R292 (= R292), E296 (= E296), R338 (= R336)
- binding 3-(3-methylbut-2-en-1-yl)-3H-purin-6-amine: K159 (= K158), Y203 (≠ F202), L204 (≠ V203), L278 (= L277)
2w6qA Crystal structure of biotin carboxylase from e. Coli in complex with the triazine-2,4-diamine fragment (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/446 of 2w6qA
- active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E288), N290 (= N290), R292 (= R292), E296 (= E296), R338 (= R336)
- binding 6-(2-phenoxyethoxy)-1,3,5-triazine-2,4-diamine: I157 (= I156), K159 (= K158), E201 (= E200), K202 (= K201), Y203 (≠ F202), L204 (≠ V203), H236 (= H235), L278 (= L277)
2w6pA Crystal structure of biotin carboxylase from e. Coli in complex with 5-methyl-6-phenyl-quinazoline-2,4-diamine (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/446 of 2w6pA
- active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E288), N290 (= N290), R292 (= R292), E296 (= E296), R338 (= R336)
- binding 5-methyl-6-phenylquinazoline-2,4-diamine: K159 (= K158), Y203 (≠ F202), L204 (≠ V203), Q233 (= Q232), H236 (= H235), L278 (= L277), I437 (≠ T436)
2w6mA Crystal structure of biotin carboxylase from e. Coli in complex with amino-oxazole fragment series (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/446 of 2w6mA
- active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E288), N290 (= N290), R292 (= R292), E296 (= E296), R338 (= R336)
- binding (2-amino-1,3-oxazol-5-yl)-(3-bromophenyl)methanone: I157 (= I156), K159 (= K158), M169 (= M168), E201 (= E200), K202 (= K201), Y203 (≠ F202), H236 (= H235), L278 (= L277), I437 (≠ T436)
2v5aA Crystal structure of biotin carboxylase from e.Coli in complex with potent inhibitor 3 (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/446 of 2v5aA
- active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E288), N290 (= N290), R292 (= R292), E296 (= E296), R338 (= R336)
- binding 7-(2,5-dihydropyrrol-1-yl)-6-phenyl-pyrido[6,5-d]pyrimidin-2-amine: I157 (= I156), K159 (= K158), M169 (= M168), E201 (= E200), Y203 (≠ F202), L204 (≠ V203), Q233 (= Q232), H236 (= H235), L278 (= L277), I437 (≠ T436)
2v58A Crystal structure of biotin carboxylase from e.Coli in complex with potent inhibitor 1 (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/446 of 2v58A
- active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E288), N290 (= N290), R292 (= R292), E296 (= E296), R338 (= R336)
- binding 6-(2,6-dibromophenyl)pyrido[2,3-d]pyrimidine-2,7-diamine: I157 (= I156), K159 (= K158), E201 (= E200), Y203 (≠ F202), L204 (≠ V203), H209 (= H208), Q233 (= Q232), H236 (= H235), L278 (= L277), I437 (≠ T436)
3jziA Crystal structure of biotin carboxylase from e. Coli in complex with benzimidazole series (see paper)
58% identity, 98% coverage: 1:440/449 of query aligns to 1:441/445 of 3jziA
- active site: K116 (= K116), K159 (= K158), D196 (= D195), H209 (= H208), R235 (= R234), T274 (= T273), E276 (= E275), E288 (= E288), N290 (= N290), R292 (= R292), E296 (= E296), R338 (= R336)
- binding 7-amino-2-[(2-chlorobenzyl)amino]-1-{[(1S,2S)-2-hydroxycycloheptyl]methyl}-1H-benzimidazole-5-carboxamide: K116 (= K116), K159 (= K158), A160 (= A159), G164 (= G163), G165 (= G164), M169 (= M168), Y199 (= Y198), E201 (= E200), K202 (= K201), Y203 (≠ F202), H209 (= H208), Q233 (= Q232), H236 (= H235), L278 (= L277), I287 (≠ M287), E288 (= E288)
Query Sequence
>Echvi_0191 Echvi_0191 acetyl-CoA carboxylase, biotin carboxylase subunit
MFNKILIANRGEIALRIIRTCKEMGIKTVAVYSTADKDSLHVRFADEAVCIGAAPSRESY
LNIPRVIAAAEITNADAIHPGYGFLSENAEFSKICEEYNIKFIGASSEMIDKMGDKATAK
ATMKAAGVPTIPGSEGLLDSIEQGIKIANEMGYPVILKATAGGGGRGMRIVREEKEFKKA
WDDARQESGAAFGNDGLYLEKFVEEPRHIEIQVVGDNTGKACHLSERDCSIQRRHQKLVE
ETPSPFITDELRDAMGKAAIKGAEAIGYEGAGTIEFLVDKHRNFYFMEMNTRIQVEHPIT
EEVTDYDLIKEQIKVAAGIPISGQNYYPKLYAMECRINAEDPANGFRPSPGKINNLHLPG
GRGVRVDSHVYAGYIIPPNYDSMIAKLIVSGQSREEVIVRMKRALEEFVIDGIKTTIPFH
IALLEDEQFKAGNFTTKFLETFDFSVIKG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory