SitesBLAST

P46354 Purine nucleoside phosphorylase 1; PNP 1; Inosine phosphorylase; Inosine-guanosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; Pu-NPase I; EC 2.4.2.1 from Bacillus subtilis (strain 168) (see paper)
49% identity, 95% coverage: 12:279/282 of query aligns to 3:270/271 of P46354

query
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S28 (≠ T37) modified: Phosphoserine

1a9sA Bovine purine nucleoside phosphorylase complexed with inosine and sulfate (see paper)
47% identity, 98% coverage: 1:276/282 of query aligns to 1:279/282 of 1a9sA

query
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1a9sA

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active site: S33 (≠ T37), H64 (= H68), H86 (= H90), Y88 (= Y92), E89 (= E93), A116 (= A120), M219 (= M218), S220 (= S219), N243 (vs. gap), V245 (vs. gap), H257 (≠ K251)
binding inosine: S33 (≠ T37), H86 (= H90), Y88 (= Y92), A116 (= A120), A117 (= A121), G118 (= G122), F200 (≠ L199), E201 (= E200), V217 (= V216), G218 (= G217), M219 (= M218), T242 (= T241), N243 (vs. gap), H257 (≠ K251), V260 (= V257)

1a9qA Bovine purine nucleoside phosphorylase complexed with inosine (see paper)
47% identity, 98% coverage: 1:276/282 of query aligns to 1:279/282 of 1a9qA

query
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1a9qA

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active site: S33 (≠ T37), H64 (= H68), H86 (= H90), Y88 (= Y92), E89 (= E93), A116 (= A120), M219 (= M218), S220 (= S219), N243 (vs. gap), V245 (vs. gap), H257 (≠ K251)
binding hypoxanthine: A116 (= A120), A117 (= A121), G118 (= G122), F200 (≠ L199), E201 (= E200), V217 (= V216), G218 (= G217), M219 (= M218), T242 (= T241), N243 (vs. gap)

1a9pA Bovine purine nucleoside phosphorylase complexed with 9-deazainosine and phosphate (see paper)
47% identity, 98% coverage: 1:276/282 of query aligns to 1:279/289 of 1a9pA

query
sites
1a9pA

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active site: S33 (≠ T37), H64 (= H68), H86 (= H90), Y88 (= Y92), E89 (= E93), A116 (= A120), M219 (= M218), S220 (= S219), N243 (vs. gap), V245 (vs. gap), H257 (≠ K251)
binding 9-deazainosine: S33 (≠ T37), H86 (= H90), Y88 (= Y92), A116 (= A120), A117 (= A121), G118 (= G122), F200 (≠ L199), E201 (= E200), V217 (= V216), G218 (= G217), M219 (= M218), T242 (= T241), N243 (vs. gap), H257 (≠ K251), E259 (= E256)
binding phosphate ion: G32 (= G36), S33 (≠ T37), H64 (= H68), R84 (= R88), H86 (= H90), N115 (= N119), A116 (= A120), S220 (= S219)

1a9oA Bovine purine nucleoside phosphorylase complexed with phosphate (see paper)
47% identity, 98% coverage: 1:276/282 of query aligns to 1:279/289 of 1a9oA

query
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1a9oA

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A

A

Q

S

T

L

F

S

D

Y

Y

K

S

D

E

I

I

P

P

C

N

F

F

P

P

I

E

S

S

T

T

V

V

E

P

T

G

H
|
H

A

A

G

G

E

R

L

L

V

V

L

F

G

G

T

I

L

L

H

N

G

G

V

R

P

A

I

C

V

V

A

M

M

M

K

Q

G

G

R
|
R

F

F

H
|
H

Y

M

Y
|
Y

E
|
E

G

G

Y

Y

D

P

M

F

K

W

A

K

V

V

T

T

F

F

P

P

V

V

R

R

V

V

M

F

R

R

Q

L

L

L

G

G

I

V

K

E

Q

T

L

L

I

V

V

V

S

T

N
|
N

A
|
A

A

A

G

G

L

L

D

N

P

P

A

N

F

F

E

E

V

V

G

G

D

D

V

I

M

M

L

L

I

I

N

R

D

D

H

H

I

I

D

N

L

L

-

P

-

G

-

F

F

S

P

G

E

E

N

N

P

P

L

L

R

R

G

G

K

P

N

N

L

E

D

E

D

R

F

F

G

G

V

V

R

R

F

F

P

P

D

A

M

M

S

S

E

D

P

A

Y

Y

D

D

Q

R

G

D

W

M

L

R

E

Q

A

K

A

A

V

H

R

S

V

T

A

W

K

K

Q

Q

E

M

G

G

-

E

-

Q

I

R

K

E

V

L

H

Q

Q

E

G

G

V

T

Y

Y

A

V

G

M

V

L

Q

G

G

G

P

P

N

N

L

F

E

E

T

T

K

V

A

A

E

E

Y

C

R

R

Y

L

L

L

R

R

T

N

I

L

G

G

A

A

D

D

A

A

V

V

G

G

M
|
M

S
|
S

T

T

V

V

P

P

E

E

V

V

I

I

V

V

A

A

R

R

Q

H

M

C

E

G

L

L

P

R

V

V

L

F

A

G

M

F

S

S

A

L

I

I

T

T

-
x
N

-

K

-
x
V

-

I

-

M

D

D

L

T

C

E

S

S

P

Q

G

G

K

K

V

A

K

N

K
x
H

I

-

S

-

I

-

Q

E

E

E

V

V

I

L

A

E

A

A

A

G

A

K

I

Q

A

A

E

A

P

Q

K

K

M

L

T

E

R

Q

I

F

I

V

A

S

S

L

L

L

I

M

active site: S33 (≠ T37), H64 (= H68), H86 (= H90), Y88 (= Y92), E89 (= E93), A116 (= A120), M219 (= M218), S220 (= S219), N243 (vs. gap), V245 (vs. gap), H257 (≠ K251)
binding phosphate ion: G32 (= G36), S33 (≠ T37), R84 (= R88), H86 (= H90), N115 (= N119), A116 (= A120), S220 (= S219)

1b8nA Purine nucleoside phosphorylase (see paper)
51% identity, 88% coverage: 29:276/282 of query aligns to 24:278/278 of 1b8nA

query
sites
1b8nA

P

P

E

Q

V

V

G

A

I

V

I

I

L

C

G

G

T
x
S

G

G

L

L

G

G

Q

G

L

L

I

V

D

N

K

K

I

L

E

T

V

Q

E

A

A

Q

S

T

L

F

S

D

Y

Y

K

S

D

E

I

I

P

P

C

N

F

F

P

P

I

E

S

S

T

T

V

V

E

P

T

G

H
|
H

A

A

G

G

E

R

L

L

V

V

L

F

G

G

T

I

L

L

H

N

G

G

V

R

P

A

I

C

V

V

A

M

M

M

K

Q

G

G

R

R

F

F

H
|
H

Y

M

Y
|
Y

E
|
E

G

G

Y

Y

D

P

M

F

K

W

A

K

V

V

T

T

F

F

P

P

V

V

R

R

V

V

M

F

R

R

Q

L

L

L

G

G

I

V

K

E

Q

T

L

L

I

V

V

V

S

T

N

N

A
|
A

G
|
G

G

G

L

L

D

N

P

P

A

N

F

F

E

E

V

V

G

G

D

D

V

I

M

M

L

L

I

I

N

R

D

D

H

H

I

I

D

N

L

L

-

P

-

G

-

F

F

S

P

G

E

E

N

N

P

P

L

L

R

R

G

G

K

P

N

N

L

E

D

E

D

R

F

F

G

G

V

V

R

R

F

F

P

P

D

A

M

M

S

S

E

D

P

A

Y

Y

D

D

Q

R

G

D

W

M

L

R

E

Q

A

K

A

A

V

H

R

S

V

T

A

W

K

K

Q

Q

E

M

G

G

-

E

-

Q

I

R

K

E

V

L

H

Q

Q

E

G

G

V

T

Y

Y

A

V

G

M

V

L

Q

G

G

G

P

P

N

N

L
x
F

E
|
E

T

T

K

V

A

A

E

E

Y

C

R

R

Y

L

L

L

R

R

T

N

I

L

G

G

A

A

D

D

A

A

V
|
V

G
|
G

M
|
M

S
|
S

T

T

V

V

P

P

E

E

V

V

I

I

V

V

A

A

R

R

Q

H

M

C

E

G

L

L

P

R

V

V

L

F

A

G

M

F

S

S

A

L

I

I

T
|
T

-
x
N

-

K

-
x
V

-

I

-

M

D

D

L

Y

C

E

S

S

P

Q

G

G

K

K

V

A

K

N

K
x
H

I

-

S

-

I

-

Q

E

E

E

V
|
V

I

L

A

E

A

A

A

G

A

K

I

Q

A

A

E

A

P

Q

K

K

M

L

T

E

R

Q

I

F

I

V

A

S

S

L

L

L

I

M

active site: S32 (≠ T37), H63 (= H68), H85 (= H90), Y87 (= Y92), E88 (= E93), A115 (= A120), M218 (= M218), S219 (= S219), N242 (vs. gap), V244 (vs. gap), H256 (≠ K251)
binding 1,4-dideoxy-1,4-imino-1-(s)-(9-deazaguanin-9-yl)-d-ribitol: S32 (≠ T37), H85 (= H90), Y87 (= Y92), A115 (= A120), A116 (= A121), G117 (= G122), F199 (≠ L199), E200 (= E200), V216 (= V216), G217 (= G217), M218 (= M218), T241 (= T241), N242 (vs. gap), H256 (≠ K251), V259 (= V257)

1b8oA Purine nucleoside phosphorylase (see paper)
51% identity, 88% coverage: 29:276/282 of query aligns to 23:277/280 of 1b8oA

query
sites
1b8oA

P

P

E

Q

V

V

G

A

I

V

I

I

L

C

G
|
G

T
x
S

G

G

L

L

G

G

Q

G

L

L

I

V

D

N

K

K

I

L

E

T

V

Q

E

A

A

Q

S

T

L

F

S

D

Y

Y

K

S

D

E

I

I

P

P

C

N

F

F

P

P

I

E

S

S

T

T

V

V

E

P

T

G

H
|
H

A

A

G

G

E

R

L

L

V

V

L

F

G

G

T

I

L

L

H

N

G

G

V

R

P

A

I

C

V

V

A

M

M

M

K

Q

G

G

R
|
R

F

F

H
|
H

Y

M

Y
|
Y

E
|
E

G

G

Y

Y

D

P

M

F

K

W

A

K

V

V

T

T

F

F

P

P

V

V

R

R

V

V

M

F

R

R

Q

L

L

L

G

G

I

V

K

E

Q

T

L

L

I

V

V

V

S

T

N
|
N

A
|
A

G
|
G

G

G

L

L

D

N

P

P

A

N

F

F

E

E

V

V

G

G

D

D

V

I

M

M

L

L

I

I

N

R

D

D

H

H

I

I

D

N

L

L

-

P

-

G

-

F

F

S

P

G

E

E

N

N

P

P

L

L

R

R

G

G

K

P

N

N

L

E

D

E

D

R

F

F

G

G

V

V

R

R

F

F

P

P

D

A

M

M

S

S

E

D

P

A

Y

Y

D

D

Q

R

G

D

W

M

L

R

E

Q

A

K

A

A

V

H

R

S

V

T

A

W

K

K

Q

Q

E

M

G

G

-

E

-

Q

I

R

K

E

V

L

H

Q

Q

E

G

G

V

T

Y

Y

A

V

G

M

V

L

Q

G

G

G

P

P

N

N

L
x
F

E
|
E

T

T

K

V

A

A

E

E

Y

C

R

R

Y

L

L

L

R

R

T

N

I

L

G

G

A

A

D

D

A

A

V
|
V

G
|
G

M
|
M

S
|
S

T

T

V

V

P

P

E

E

V

V

I

I

V

V

A

A

R

R

Q

H

M

C

E

G

L

L

P

R

V

V

L

F

A

G

M

F

S

S

A

L

I

I

T
|
T

-
x
N

-

K

-
x
V

-

I

-

M

D

D

L

Y

C

E

S

S

P

Q

G

G

K

K

V

A

K

N

K
x
H

I

-

S

-

I

-

Q

E

E

E

V
|
V

I

L

A

E

A

A

A

G

A

K

I

Q

A

A

E

A

P

Q

K

K

M

L

T

E

R

Q

I

F

I

V

A

S

S

L

L

L

I

M

active site: S31 (≠ T37), H62 (= H68), H84 (= H90), Y86 (= Y92), E87 (= E93), A114 (= A120), M217 (= M218), S218 (= S219), N241 (vs. gap), V243 (vs. gap), H255 (≠ K251)
binding 1,4-dideoxy-4-aza-1-(s)-(9-deazahypoxanthin-9-yl)-d-ribitol: S31 (≠ T37), H84 (= H90), Y86 (= Y92), A114 (= A120), A115 (= A121), G116 (= G122), F198 (≠ L199), E199 (= E200), V215 (= V216), G216 (= G217), M217 (= M218), T240 (= T241), N241 (vs. gap), H255 (≠ K251), V258 (= V257)
binding phosphate ion: G30 (= G36), S31 (≠ T37), H62 (= H68), R82 (= R88), H84 (= H90), N113 (= N119), A114 (= A120), S218 (= S219)

P55859 Purine nucleoside phosphorylase; PNP; Inosine phosphorylase; Inosine-guanosine phosphorylase; EC 2.4.2.1 from Bos taurus (Bovine) (see paper)
51% identity, 88% coverage: 29:276/282 of query aligns to 25:279/289 of P55859

query
sites
P55859

P

P

E

Q

V

V

G

A

I

V

I

I

L

C

G

G

T
x
S

G

G

L

L

G

G

Q

G

L

L

I

V

D

N

K

K

I

L

E

T

V

Q

E

A

A

Q

S

T

L

F

S

D

Y

Y

K

S

D

E

I

I

P

P

C

N

F

F

P

P

I

E

S

S

T

T

V

V

E

P

T

G

H
|
H

A

A

G

G

E

R

L

L

V

V

L

F

G

G

T

I

L

L

H

N

G

G

V

R

P

A

I

C

V

V

A

M

M

M

K

Q

G

G

R
|
R

F
|
F

H
|
H

Y

M

Y
|
Y

E

E

G

G

Y

Y

D

P

M

F

K

W

A

K

V

V

T

T

F

F

P

P

V

V

R

R

V

V

M

F

R

R

Q

L

L

L

G

G

I

V

K

E

Q

T

L

L

I

V

V

V

S

T

N

N

A
|
A

A

A

G

G

L

L

D

N

P

P

A

N

F

F

E

E

V

V

G

G

D

D

V

I

M

M

L

L

I

I

N

R

D

D

H

H

I

I

D

N

L

L

-

P

-

G

-

F

F

S

P

G

E

E

N

N

P

P

L

L

R

R

G

G

K

P

N

N

L

E

D

E

D

R

F

F

G

G

V

V

R

R

F

F

P

P

D

A

M

M

S

S

E

D

P

A

Y

Y

D

D

Q

R

G

D

W

M

L

R

E

Q

A

K

A

A

V

H

R

S

V

T

A

W

K

K

Q

Q

E

M

G

G

-

E

-

Q

I

R

K

E

V

L

H

Q

Q

E

G

G

V

T

Y

Y

A

V

G

M

V

L

Q

G

G

G

P

P

N

N

L

F

E
|
E

T

T

K

V

A

A

E

E

Y

C

R

R

Y

L

L

L

R

R

T

N

I

L

G

G

A

A

D

D

A

A

V

V

G

G

M
|
M

S
|
S

T

T

V

V

P

P

E

E

V

V

I

I

V

V

A

A

R

R

Q

H

M

C

E

G

L

L

P

R

V

V

L

F

A

G

M

F

S

S

A

L

I

I

T

T

-
x
N

-

K

-

V

-

I

-

M

D

D

L

Y

C

E

S

S

P

Q

G

G

K

K

V

A

K

N

K
x
H

I

-

S

-

I

-

Q

E

E

E

V

V

I

L

A

E

A

A

A

G

A

K

I

Q

A

A

E

A

P

Q

K

K

M

L

T

E

R

Q

I

F

I

V

A

S

S

L

L

L

I

M

S33 (≠ T37) binding
H64 (= H68) binding
RFH 84:86 (= RFH 88:90) binding
Y88 (= Y92) binding
A116 (= A120) binding
E201 (= E200) binding
M219 (= M218) binding
S220 (= S219) binding
N243 (vs. gap) binding
H257 (≠ K251) binding

1a9tA Bovine purine nucleoside phosphorylase complexed with 9-deazainosine and phosphate (see paper)
51% identity, 88% coverage: 29:276/282 of query aligns to 25:279/284 of 1a9tA

query
sites
1a9tA

P

P

E

Q

V

V

G

A

I

V

I

I

L

C

G
|
G

T
x
S

G

G

L

L

G

G

Q

G

L

L

I

V

D

N

K

K

I

L

E

T

V

Q

E

A

A

Q

S

T

L

F

S

D

Y

Y

K

S

D

E

I

I

P

P

C

N

F

F

P

P

I

E

S

S

T

T

V

V

E

P

T

G

H
|
H

A

A

G

G

E

R

L

L

V

V

L

F

G

G

T

I

L

L

H

N

G

G

V

R

P

A

I

C

V

V

A

M

M

M

K

Q

G

G

R
|
R

F

F

H
|
H

Y

M

Y
|
Y

E
|
E

G

G

Y

Y

D

P

M

F

K

W

A

K

V

V

T

T

F

F

P

P

V

V

R

R

V

V

M

F

R

R

Q

L

L

L

G

G

I

V

K

E

Q

T

L

L

I

V

V

V

S

T

N
|
N

A
|
A

G
|
G

G

G

L

L

D

N

P

P

A

N

F

F

E

E

V

V

G

G

D

D

V

I

M

M

L

L

I

I

N

R

D

D

H

H

I

I

D

N

L

L

-

P

-

G

-

F

F

S

P

G

E

E

N

N

P

P

L

L

R

R

G

G

K

P

N

N

L

E

D

E

D

R

F

F

G

G

V

V

R

R

F

F

P

P

D

A

M

M

S

S

E

D

P

A

Y

Y

D

D

Q

R

G

D

W

M

L

R

E

Q

A

K

A

A

V

H

R

S

V

T

A

W

K

K

Q

Q

E

M

G

G

-

E

-

Q

I

R

K

E

V

L

H

Q

Q

E

G

G

V

T

Y

Y

A

V

G

M

V

L

Q

G

G

G

P

P

N

N

L
x
F

E
|
E

T

T

K

V

A

A

E

E

Y

C

R

R

Y

L

L

L

R

R

T

N

I

L

G

G

A

A

D

D

A

A

V
|
V

G
|
G

M
|
M

S
|
S

T

T

V

V

P

P

E

E

V

V

I

I

V

V

A

A

R

R

Q

H

M

C

E

G

L

L

P

R

V

V

L

F

A

G

M

F

S

S

A

L

I

I

T
|
T

-
x
N

-

K

-
x
V

-

I

-

M

D

D

L

T

C

E

S

S

P

Q

G

G

K

K

V

A

K

N

K
x
H

I

-

S

-

I

-

Q

E

E

E

V
|
V

I

L

A

E

A

A

A

G

A

K

I

Q

A

A

E

A

P

Q

K

K

M

L

T

E

R

Q

I

F

I

V

A

S

S

L

L

L

I

M

active site: S33 (≠ T37), H64 (= H68), H86 (= H90), Y88 (= Y92), E89 (= E93), A116 (= A120), M219 (= M218), S220 (= S219), N243 (vs. gap), V245 (vs. gap), H257 (≠ K251)
binding hypoxanthine: A116 (= A120), A117 (= A121), G118 (= G122), F200 (≠ L199), E201 (= E200), V217 (= V216), G218 (= G217), M219 (= M218), T242 (= T241), N243 (vs. gap), V260 (= V257)
binding 1-O-phosphono-alpha-D-ribofuranose: G32 (= G36), S33 (≠ T37), H64 (= H68), R84 (= R88), H86 (= H90), Y88 (= Y92), N115 (= N119), A116 (= A120), F200 (≠ L199), M219 (= M218), S220 (= S219), H257 (≠ K251), V260 (= V257)

1fxuA Purine nucleoside phosphorylase from calf spleen in complex with n(7)- acycloguanosine inhibitor and a phosphate ion (see paper)
50% identity, 88% coverage: 29:276/282 of query aligns to 22:273/278 of 1fxuA

query
sites
1fxuA

P

P

E

Q

V

V

G

A

I

V

I

I

L

C

G
|
G

T
x
S

G

G

L

L

G

G

Q

G

L

L

I

V

D

N

K

K

I

L

E

T

V

Q

E

A

A

Q

S

T

L

F

S

D

Y

Y

K

S

D

E

I

I

P

P

C

N

F

F

P

P

I

E

S

S

T

T

V

V

E

P

T

G

H
|
H

A

A

G

G

E

R

L

L

V

V

L

F

G

G

T

I

L

L

H

N

G

G

V

R

P

A

I

C

V

V

A

M

M

M

K

Q

G

G

R
|
R

F

F

H
|
H

Y

M

Y
|
Y

E
|
E

G

G

Y

Y

D

P

M

F

K

W

A

K

V

V

T

T

F

F

P

P

V

V

R

R

V

V

M

F

R

R

Q

L

L

L

G

G

I

V

K

E

Q

T

L

L

I

V

V

V

S

T

N
|
N

A
|
A

G
|
G

G

G

L

L

D

N

P

P

A

N

F

F

E

E

V

V

G

G

D

D

V

I

M

M

L

L

I

I

N

R

D

D

H

H

I

I

D

N

L

L

-

P

-

G

-

F

F

S

P

G

E

Q

N

N

P

P

L

L

R

R

G

G

K

P

N

N

L

E

D

E

D

R

F

F

G

G

V

V

R

R

F

F

P

P

D

A

M

M

S

S

E

D

P

A

Y

Y

D

D

Q

R

G

D

W

M

L

R

E

Q

A

K

A

A

V

H

R

S

V

T

A

W

K

K

Q

Q

E

M

G

G

-

E

-

Q

I

R

K

E

V

L

H

Q

Q

E

G

G

V

T

Y

Y

A

V

G

M

V

L

Q

G

G

G

P

P

N

N

L
x
F

E
|
E

T

T

K

V

A

A

E

E

Y

C

R

R

Y

L

L

L

R

R

T

N

I

L

G

G

A

A

D

D

A

A

V
|
V

G
|
G

M
|
M

S
|
S

T

T

V

V

P

P

E

E

V

V

I

I

V

V

A

A

R

R

Q

H

M

C

E

G

L

L

P

R

V

V

L

F

A

G

M

F

S

S

A

L

I

I

T
|
T

D
x
N

-

K

-
x
V

L

I

C

M

S

D

P

T

G

G

K

K

V

A

K

N

K
x
H

I

-

S

-

I

-

Q

E

E

E

V
|
V

I

L

A

E

A

A

A

G

A

K

I

Q

A

A

E

A

P

Q

K

K

M

L

T

E

R

Q

I

F

I

V

A

S

S

L

L

L

I

M

active site: S30 (≠ T37), H61 (= H68), H83 (= H90), Y85 (= Y92), E86 (= E93), A113 (= A120), M216 (= M218), S217 (= S219), N240 (≠ D242), V242 (vs. gap), H251 (≠ K251)
binding 2-amino-7-[2-(2-hydroxy-1-hydroxymethyl-ethylamino)-ethyl]-1,7-dihydro-purin-6-one: S30 (≠ T37), H83 (= H90), Y85 (= Y92), A113 (= A120), A114 (= A121), G115 (= G122), F197 (≠ L199), E198 (= E200), V214 (= V216), G215 (= G217), M216 (= M218), T239 (= T241), N240 (≠ D242), V242 (vs. gap), H251 (≠ K251), V254 (= V257)
binding phosphate ion: G29 (= G36), S30 (≠ T37), H61 (= H68), R81 (= R88), H83 (= H90), N112 (= N119), A113 (= A120), S217 (= S219)

4nsnA Crystal structure of purine nucleoside phosphorylase from porphyromonas gingivalis atcc 33277, nysgrc target 030972, orthorhombic symmetry
49% identity, 92% coverage: 17:276/282 of query aligns to 11:270/273 of 4nsnA

query
sites
4nsnA

A

A

V

A

T

S

Y

F

I

L

S

S

E

S

Q

R

H

L

P

P

F

G

I

D

P

A

E

K

V

T

G

A

I

I

L

L

G

G

T
x
S

G

G

L

L

G

G

Q

E

L

L

I

A

D

E

K

K

I

I

E

E

V

N

E

K

A

T

S

V

L

I

S

P

Y

Y

K

N

D

E

I

I

P

P

C

H

F

F

P

A

I

Q

S

A

T

T

V

A

E

V

T

G

H
|
H

A

K

G

G

E

N

L

I

V

I

L

G

G

G

T

I

L

L

H

G

G

G

V

T

P

P

I

V

V

V

A

A

M

M

K

Q

G

G

R

R

F

F

H
|
H

Y

Y

Y
|
Y

E
|
E

G

G

Y

Y

D

S

M

M

K

D

A

Q

V

V

T

T

F

F

P

P

V

I

R

R

V

V

M

M

R

K

Q

L

L

L

G

G

I

I

K

E

Q

N

L

L

I

F

V

V

S

S

N

N

A
|
A

G
|
G

G

G

L

I

D

N

P

T

A

S

F

F

E

K

V

V

G

G

D

D

V

L

M

M

L

I

I

I

N

C

D

D

H

H

I

I

D

N

L

N

F

L

P

P

E

-

N

N

P

P

L

L

R

I

G

G

K

P

N

N

L

M

D

D

D

M

F

F

G

G

V

V

R

R

F

F

P

P

D

D

M

M

S

T

E

R

P

A

Y

Y

D

D

Q

R

G

E

W

F

L

I

E

A

A

K

A

A

V

K

R

G

V

I

A

A

K

Q

Q

E

E

L

G

N

I

I

K

P

V

V

H

K

Q

E

G

G

V

V

Y

Y

A

V

G

G

V

L

Q

T

G

G

P

P

N

S

L
x
Y

E
|
E

T

T

K

P

A

A

E

E

Y
|
Y

R

K

Y

F

L

W

R

G

T

Q

I

V

G

G

A

G

D

D

A

A

V
x
I

G
|
G

M
|
M

S
|
S

T

T

V

V

P

P

E

E

V

V

I

I

V

V

A

A

R

R

Q

H

M

T

E

G

L

I

P

R

V

V

L

F

A

G

M

M

S

S

A

V

I

I

T
|
T

D
x
N

L

E

C

G

S

Y

P

H

G

F

K

A

V

D

K

D

K

F

I

V

S

N

I

D

-
x
E

Q

Q

E

D

V
|
V

I

I

A

R

A

A

A

N

I

A

A

A

E

S

P

E

K

K

M

M

T

G

R

A

I

I

I

F

A

A

S

R

L

L

I

I

active site: S31 (≠ T37), H62 (= H68), H84 (= H90), Y86 (= Y92), E87 (= E93), A114 (= A120), M211 (= M218), S212 (= S219), N235 (≠ D242), E248 (vs. gap)
binding adenine: A114 (= A120), A115 (= A121), G116 (= G122), Y192 (≠ L199), E193 (= E200), Y198 (= Y205), I209 (≠ V216), G210 (= G217), M211 (= M218), T234 (= T241), N235 (≠ D242), V251 (= V257)

1lv8A Crystal structure of calf spleen purine nucleoside phosphorylase in a new space group with full trimer in the asymmetric unit (see paper)
55% identity, 76% coverage: 29:242/282 of query aligns to 23:241/265 of 1lv8A

query
sites
1lv8A

P

P

E

Q

V

V

G

A

I

V

I

I

L

C

G
|
G

T
x
S

G

G

L

L

G

G

Q

G

L

L

I

V

D

N

K

K

I

L

E

T

V

Q

E

A

A

Q

S

T

L

F

S

D

Y

Y

K
x
S

D

E

I

I

P

P

C

N

F

F

P

P

I
x
E

S

S

T

T

V

V

E

P

T

G

H
|
H

A

A

G

G

E

R

L

L

V

V

L

F

G

G

T

I

L

L

H

N

G

G

V

R

P

A

I

C

V

V

A

M

M

M

K

Q

G

G

R
|
R

F

F

H
|
H

Y

M

Y
|
Y

E

E

G

G

Y

Y

D

P

M

F

K

W

A

K

V

V

T

T

F

F

P

P

V

V

R

R

V

V

M

F

R

R

Q

L

L

L

G

G

I

V

K

E

Q

T

L

L

I

V

V

V

S

T

N
|
N

A
|
A

G
|
G

G

G

L

L

D

N

P

P

A

N

F

F

E

E

V

V

G

G

D

D

V

I

M

M

L

L

I

I

N

R

D

D

H

H

I

I

D

N

L

L

-

P

-

G

-

F

F

S

P

G

E

Q

N

N

P

P

L

L

R

R

G

G

K

P

N

N

L

E

D

E

D

R

F

F

G

G

V

V

R

R

F

F

P

P

D

A

M

M

S

S

E

D

P

A

Y

Y

D

D

Q

R

G

D

W

M

L

R

E

Q

A

K

A

A

V

H

R

S

V

T

A

W

K

K

Q

Q

E

M

G

G

-

E

-

Q

I

R

K

E

V

L

H

Q

Q

E

G

G

V

T

Y

Y

A

V

G

M

V

L

Q

G

G

G

P

P

N

N

L
x
F

E
|
E

T

T

K

V

A

A

E

E

Y

C

R

R

Y

L

L

L

R

R

T

N

I

L

G

G

A

A

D

D

A

A

V
|
V

G
|
G

M
|
M

S
|
S

T

T

V

V

P

P

E

E

V

V

I

I

V

V

A

A

R

R

Q

H

M

C

E

G

L

L

P

R

V

V

L

F

A

G

M

F

S

S

A

L

I

I

T

T

D
x
N

active site: S31 (≠ T37), H62 (= H68), Y86 (= Y92), A114 (= A120), M217 (= M218), S218 (= S219)
binding 2,6-diamino-(s)-9-[2-(phosphonomethoxy)propyl]purine: G30 (= G36), S31 (≠ T37), R82 (= R88), H84 (= H90), N113 (= N119), A114 (= A120), A115 (= A121), G116 (= G122), F198 (≠ L199), E199 (= E200), V215 (= V216), G216 (= G217), M217 (= M218), S218 (= S219), N241 (≠ D242)
binding calcium ion: S49 (≠ K55), E56 (≠ I62)

4earA Crystal structure of purine nucleoside phosphorylase (w16y, w94y, w178y, h257w) mutant from human complexed with dadme-immg and phosphate (see paper)
47% identity, 94% coverage: 12:276/282 of query aligns to 5:276/283 of 4earA

query
sites
4earA

E

E

Q

D

I

Y

T

K

A

N

A

T

V

A

T

E

Y

Y

I

L

S

L

E

S

Q

H

H

T

P

K

F

H

I

R

P

P

E

Q

V

V

G

A

I

I

L

C

G
|
G

T
x
S

G

G

L

L

G

G

Q

G

L

L

I

T

D

D

K

K

I

L

E

T

V

Q

E

A

A

Q

S

I

L

F

S

D

Y

Y

K

S

D

E

I

I

P

P

C

N

F

F

P

P

I

R

S

S

T

T

V

V

E

P

T

G

H
|
H

A

A

G

G

E

R

L

L

V

V

L

F

G

G

T

F

L

L

H

N

G

G

V

R

P

A

I

C

V

V

A

M

M

M

K

Q

G

G

R
|
R

F

F

H
|
H

Y

M

Y
|
Y

E
|
E

G

G

Y

Y

D

P

M

L

K

Y

A

K

V

V

T

T

F

F

P

P

V

V

R

R

V

V

M

F

R

H

Q

L

L

L

G

G

I

V

K

D

Q

T

L

L

I

V

V

V

S

T

N
|
N

A
|
A

G
|
G

G

G

L

L

D

N

P

P

A

K

F

F

E

E

V

V

G

G

D

D

V

I

M

M

L

L

I

I

N

R

D

D

H

H

I

I

D

N

L

L

-

P

-

G

-

F

F

S

P

G

E

Q

N

N

P

P

L

L

R

R

G

G

K

P

N

N

L

D

D

E

D

R

F

F

G

G

V

D

R

R

F

F

P

P

D

A

M

M

S

S

E

D

P

A

Y

Y

D

D

Q

R

G

T

W

M

L

R

E

Q

A

R

A

A

V

L

R

S

V

T

A

Y

K

K

Q

Q

E

M

G

G

-

E

-

Q

I

R

K

E

V

L

H

Q

Q

E

G

G

V

T

Y

Y

A

V

G

M

V

V

Q

A

G

G

P

P

N

S

L
x
F

E
|
E

T

T

K

V

A

A

E

E

Y

C

R

R

Y

V

L

L

R

Q

T

K

I

L

G

G

A

A

D

D

A

A

V
|
V

G
|
G

M
|
M

S
|
S

T

T

V

V

P

P

E

E

V

V

I

I

V

V

A

A

R

R

Q

H

M

C

E

G

L

L

P

R

V

V

L

F

A

G

M

F

S

S

A

L

I

I

T
|
T

D
x
N

-

K

-
x
V

L

I

C

M

S

D

P

Y

G

E

K

S

V

L

K

E

K

K

I

A

S

N

I
x
W

Q

E

E

E

V
|
V

I

L

A

A

A

G

A

K

I

Q

A

A

E

A

P

Q

K

K

M

L

T

E

R

Q

I

F

I

V

A

S

S

I

L

L

I

M

active site: S30 (≠ T37), H61 (= H68), H83 (= H90), Y85 (= Y92), E86 (= E93), A113 (= A120), M216 (= M218), S217 (= S219), N240 (≠ D242), V242 (vs. gap), W254 (≠ I254)
binding 2-amino-7-{[(3R,4R)-3-hydroxy-4-(hydroxymethyl)pyrrolidin-1-yl]methyl}-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one: S30 (≠ T37), H83 (= H90), Y85 (= Y92), A113 (= A120), A114 (= A121), G115 (= G122), F197 (≠ L199), E198 (= E200), V214 (= V216), G215 (= G217), M216 (= M218), T239 (= T241), N240 (≠ D242), W254 (≠ I254), V257 (= V257)
binding phosphate ion: G29 (= G36), S30 (≠ T37), H61 (= H68), R81 (= R88), H83 (= H90), N112 (= N119), A113 (= A120), S217 (= S219)

3inyA Crystal structure of human purine nucleoside phosphorylase in complex with 7-deazaguanine (see paper)
50% identity, 88% coverage: 29:276/282 of query aligns to 24:278/288 of 3inyA

query
sites
3inyA

P

P

E

Q

V

V

G

A

I

I

L

C

G

G

T
x
S

G

G

L

L

G

G

Q

G

L

L

I

T

D

D

K

K

I

L

E

T

V

Q

E

A

A

Q

S

I

L

F

S

D

Y

Y

K

S

D

E

I

I

P

P

C

N

F

F

P

P

I

R

S

S