SitesBLAST
Comparing Echvi_0304 FitnessBrowser__Cola:Echvi_0304 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8swtA Structure of bacteroides fragilis pnp bound to transition state analog immucillin h and sulfate (see paper)
54% identity, 95% coverage: 11:278/282 of query aligns to 11:277/278 of 8swtA
- binding 1,4-dideoxy-4-aza-1-(s)-(9-deazahypoxanthin-9-yl)-d-ribitol: H90 (= H90), Y92 (= Y92), A120 (= A120), S121 (≠ A121), G122 (= G122), F198 (≠ L199), E199 (= E200), Y204 (= Y205), G216 (= G217), M217 (= M218), D241 (= D242), H253 (≠ I254)
4m1eB Crystal structure of purine nucleoside phosphorylase i from planctomyces limnophilus dsm 3776, nysgrc target 029364.
52% identity, 95% coverage: 8:276/282 of query aligns to 1:269/273 of 4m1eB
- active site: T30 (= T37), H61 (= H68), H83 (= H90), Y85 (= Y92), E86 (= E93), A113 (= A120), M211 (= M218), S212 (= S219), D235 (= D242), C237 (= C244), L247 (≠ I254)
- binding pyridine-2-carboxylic acid: N118 (≠ D125), Q120 (≠ A127), R169 (≠ V176), H178 (≠ K185), P180 (≠ H187), P180 (≠ H187)
- binding adenine: A114 (= A121), G115 (= G122), E193 (= E200), V209 (= V216), D235 (= D242)
4lnaA Crystal structure of purine nucleoside phosphorylase i from spirosoma linguale dsm 74, nysgrc target 029362
52% identity, 94% coverage: 11:276/282 of query aligns to 3:266/269 of 4lnaA
- active site: T29 (= T37), H79 (= H90), Y81 (= Y92), E82 (= E93), A109 (= A120), M208 (= M218), S209 (= S219), D232 (= D242), C234 (= C244), L244 (≠ I254)
- binding adenine: A110 (= A121), G111 (= G122), E190 (= E200), M208 (= M218), D232 (= D242)
P46354 Purine nucleoside phosphorylase 1; PNP 1; Inosine phosphorylase; Inosine-guanosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; Pu-NPase I; EC 2.4.2.1 from Bacillus subtilis (strain 168) (see paper)
49% identity, 95% coverage: 12:279/282 of query aligns to 3:270/271 of P46354
- S28 (≠ T37) modified: Phosphoserine
8swuA Structure of clostridium perfringens pnp bound to transition state analog immucillin h and sulfate (see paper)
45% identity, 96% coverage: 7:278/282 of query aligns to 1:268/269 of 8swuA
- binding 1,4-dideoxy-4-aza-1-(s)-(9-deazahypoxanthin-9-yl)-d-ribitol: Y82 (= Y92), A110 (= A120), A111 (= A121), G112 (= G122), Y189 (≠ L199), E190 (= E200), M208 (= M218), N232 (≠ D242), H244 (≠ I254), V247 (= V257)
1a9sA Bovine purine nucleoside phosphorylase complexed with inosine and sulfate (see paper)
47% identity, 98% coverage: 1:276/282 of query aligns to 1:279/282 of 1a9sA
- active site: S33 (≠ T37), H64 (= H68), H86 (= H90), Y88 (= Y92), E89 (= E93), A116 (= A120), M219 (= M218), S220 (= S219), N243 (vs. gap), V245 (vs. gap), H257 (≠ K251)
- binding inosine: Y88 (= Y92), A116 (= A120), G118 (= G122), F200 (≠ L199), E201 (= E200), M219 (= M218), T242 (= T241), N243 (vs. gap), H257 (≠ K251), V260 (= V257)
1a9qA Bovine purine nucleoside phosphorylase complexed with inosine (see paper)
47% identity, 98% coverage: 1:276/282 of query aligns to 1:279/282 of 1a9qA
- active site: S33 (≠ T37), H64 (= H68), H86 (= H90), Y88 (= Y92), E89 (= E93), A116 (= A120), M219 (= M218), S220 (= S219), N243 (vs. gap), V245 (vs. gap), H257 (≠ K251)
- binding hypoxanthine: A117 (= A121), G118 (= G122), F200 (≠ L199), E201 (= E200), M219 (= M218), N243 (vs. gap)
1a9pA Bovine purine nucleoside phosphorylase complexed with 9-deazainosine and phosphate (see paper)
47% identity, 98% coverage: 1:276/282 of query aligns to 1:279/289 of 1a9pA
- active site: S33 (≠ T37), H64 (= H68), H86 (= H90), Y88 (= Y92), E89 (= E93), A116 (= A120), M219 (= M218), S220 (= S219), N243 (vs. gap), V245 (vs. gap), H257 (≠ K251)
- binding 9-deazainosine: H86 (= H90), Y88 (= Y92), A116 (= A120), G118 (= G122), F200 (≠ L199), E201 (= E200), M219 (= M218), N243 (vs. gap), H257 (≠ K251), E259 (= E256)
- binding phosphate ion: G32 (= G36), S33 (≠ T37), H64 (= H68), R84 (= R88), H86 (= H90), N115 (= N119), S220 (= S219)
1a9oA Bovine purine nucleoside phosphorylase complexed with phosphate (see paper)
47% identity, 98% coverage: 1:276/282 of query aligns to 1:279/289 of 1a9oA
- active site: S33 (≠ T37), H64 (= H68), H86 (= H90), Y88 (= Y92), E89 (= E93), A116 (= A120), M219 (= M218), S220 (= S219), N243 (vs. gap), V245 (vs. gap), H257 (≠ K251)
- binding phosphate ion: G32 (= G36), R84 (= R88), N115 (= N119), S220 (= S219)
1b8nA Purine nucleoside phosphorylase (see paper)
51% identity, 88% coverage: 29:276/282 of query aligns to 24:278/278 of 1b8nA
- active site: S32 (≠ T37), H63 (= H68), H85 (= H90), Y87 (= Y92), E88 (= E93), A115 (= A120), M218 (= M218), S219 (= S219), N242 (vs. gap), V244 (vs. gap), H256 (≠ K251)
- binding 1,4-dideoxy-1,4-imino-1-(s)-(9-deazaguanin-9-yl)-d-ribitol: H85 (= H90), Y87 (= Y92), A115 (= A120), G117 (= G122), F199 (≠ L199), E200 (= E200), V216 (= V216), G217 (= G217), M218 (= M218), N242 (vs. gap), H256 (≠ K251), V259 (= V257)
1b8oA Purine nucleoside phosphorylase (see paper)
51% identity, 88% coverage: 29:276/282 of query aligns to 23:277/280 of 1b8oA
- active site: S31 (≠ T37), H62 (= H68), H84 (= H90), Y86 (= Y92), E87 (= E93), A114 (= A120), M217 (= M218), S218 (= S219), N241 (vs. gap), V243 (vs. gap), H255 (≠ K251)
- binding 1,4-dideoxy-4-aza-1-(s)-(9-deazahypoxanthin-9-yl)-d-ribitol: H84 (= H90), Y86 (= Y92), A114 (= A120), G116 (= G122), F198 (≠ L199), E199 (= E200), M217 (= M218), N241 (vs. gap), H255 (≠ K251), V258 (= V257)
- binding phosphate ion: G30 (= G36), S31 (≠ T37), R82 (= R88), H84 (= H90), N113 (= N119), S218 (= S219)
P55859 Purine nucleoside phosphorylase; PNP; Inosine phosphorylase; Inosine-guanosine phosphorylase; EC 2.4.2.1 from Bos taurus (Bovine) (see paper)
51% identity, 88% coverage: 29:276/282 of query aligns to 25:279/289 of P55859
- S33 (≠ T37) binding
- H64 (= H68) binding
- RFH 84:86 (= RFH 88:90) binding
- Y88 (= Y92) binding
- A116 (= A120) binding
- E201 (= E200) binding
- M219 (= M218) binding
- S220 (= S219) binding
- N243 (vs. gap) binding
- H257 (≠ K251) binding
1a9tA Bovine purine nucleoside phosphorylase complexed with 9-deazainosine and phosphate (see paper)
51% identity, 88% coverage: 29:276/282 of query aligns to 25:279/284 of 1a9tA
- active site: S33 (≠ T37), H64 (= H68), H86 (= H90), Y88 (= Y92), E89 (= E93), A116 (= A120), M219 (= M218), S220 (= S219), N243 (vs. gap), V245 (vs. gap), H257 (≠ K251)
- binding hypoxanthine: A116 (= A120), A117 (= A121), G118 (= G122), F200 (≠ L199), E201 (= E200), V217 (= V216), N243 (vs. gap)
- binding 1-O-phosphono-alpha-D-ribofuranose: S33 (≠ T37), R84 (= R88), H86 (= H90), Y88 (= Y92), N115 (= N119), A116 (= A120), F200 (≠ L199), M219 (= M218), S220 (= S219), V260 (= V257)
1fxuA Purine nucleoside phosphorylase from calf spleen in complex with n(7)- acycloguanosine inhibitor and a phosphate ion (see paper)
50% identity, 88% coverage: 29:276/282 of query aligns to 22:273/278 of 1fxuA
- active site: S30 (≠ T37), H61 (= H68), H83 (= H90), Y85 (= Y92), E86 (= E93), A113 (= A120), M216 (= M218), S217 (= S219), N240 (≠ D242), V242 (vs. gap), H251 (≠ K251)
- binding 2-amino-7-[2-(2-hydroxy-1-hydroxymethyl-ethylamino)-ethyl]-1,7-dihydro-purin-6-one: Y85 (= Y92), A113 (= A120), A114 (= A121), G115 (= G122), F197 (≠ L199), E198 (= E200), M216 (= M218), T239 (= T241), N240 (≠ D242), H251 (≠ K251), V254 (= V257)
- binding phosphate ion: G29 (= G36), S30 (≠ T37), R81 (= R88), H83 (= H90), N112 (= N119), S217 (= S219)
4nsnA Crystal structure of purine nucleoside phosphorylase from porphyromonas gingivalis atcc 33277, nysgrc target 030972, orthorhombic symmetry
49% identity, 92% coverage: 17:276/282 of query aligns to 11:270/273 of 4nsnA
- active site: S31 (≠ T37), H62 (= H68), H84 (= H90), Y86 (= Y92), E87 (= E93), A114 (= A120), M211 (= M218), S212 (= S219), N235 (≠ D242), E248 (vs. gap)
- binding adenine: A114 (= A120), A115 (= A121), G116 (= G122), Y192 (≠ L199), E193 (= E200), Y198 (= Y205), G210 (= G217), M211 (= M218), T234 (= T241), N235 (≠ D242)
1lv8A Crystal structure of calf spleen purine nucleoside phosphorylase in a new space group with full trimer in the asymmetric unit (see paper)
55% identity, 76% coverage: 29:242/282 of query aligns to 23:241/265 of 1lv8A
- active site: S31 (≠ T37), H62 (= H68), Y86 (= Y92), A114 (= A120), M217 (= M218), S218 (= S219)
- binding 2,6-diamino-(s)-9-[2-(phosphonomethoxy)propyl]purine: G30 (= G36), S31 (≠ T37), H84 (= H90), N113 (= N119), A114 (= A120), A115 (= A121), G116 (= G122), F198 (≠ L199), E199 (= E200), M217 (= M218), S218 (= S219), N241 (≠ D242)
- binding calcium ion: S49 (≠ K55), E56 (≠ I62)
4earA Crystal structure of purine nucleoside phosphorylase (w16y, w94y, w178y, h257w) mutant from human complexed with dadme-immg and phosphate (see paper)
47% identity, 94% coverage: 12:276/282 of query aligns to 5:276/283 of 4earA
- active site: S30 (≠ T37), H61 (= H68), H83 (= H90), Y85 (= Y92), E86 (= E93), A113 (= A120), M216 (= M218), S217 (= S219), N240 (≠ D242), V242 (vs. gap), W254 (≠ I254)
- binding 2-amino-7-{[(3R,4R)-3-hydroxy-4-(hydroxymethyl)pyrrolidin-1-yl]methyl}-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one: Y85 (= Y92), A113 (= A120), A114 (= A121), G115 (= G122), F197 (≠ L199), E198 (= E200), V214 (= V216), M216 (= M218), N240 (≠ D242), W254 (≠ I254)
- binding phosphate ion: G29 (= G36), S30 (≠ T37), R81 (= R88), H83 (= H90), N112 (= N119), S217 (= S219)
3inyA Crystal structure of human purine nucleoside phosphorylase in complex with 7-deazaguanine (see paper)
50% identity, 88% coverage: 29:276/282 of query aligns to 24:278/288 of 3inyA
- active site: S32 (≠ T37), H63 (= H68), H85 (= H90), Y87 (= Y92), E88 (= E93), A115 (= A120), M218 (= M218), S219 (= S219), N242 (≠ D242), V244 (vs. gap), H256 (≠ I254)
- binding 7-deazaguanine: A115 (= A120), A116 (= A121), G117 (= G122), F199 (≠ L199), E200 (= E200), V216 (= V216), T241 (= T241), N242 (≠ D242)
3d1vA Crystal structure of human pnp complexed with 2-mercapto(3h) quinazolinone (see paper)
50% identity, 88% coverage: 29:276/282 of query aligns to 24:278/288 of 3d1vA
- active site: S32 (≠ T37), H63 (= H68), H85 (= H90), Y87 (= Y92), E88 (= E93), A115 (= A120), M218 (= M218), S219 (= S219), N242 (≠ D242), V244 (vs. gap), H256 (≠ I254)
- binding 2-mercapto(3H)quinazolinone: A115 (= A120), G117 (= G122), F199 (≠ L199), E200 (= E200), V216 (= V216), T241 (= T241), N242 (≠ D242)
1yryE Crystal structure of human pnp complexed with mesg (see paper)
50% identity, 88% coverage: 29:276/282 of query aligns to 24:278/288 of 1yryE
- active site: S32 (≠ T37), H63 (= H68), H85 (= H90), Y87 (= Y92), E88 (= E93), A115 (= A120), M218 (= M218), S219 (= S219), N242 (≠ D242), H256 (≠ I254)
- binding 7-methyl-6-thio-guanosine: Y87 (= Y92), A115 (= A120), A116 (= A121), G117 (= G122), E200 (= E200), V216 (= V216), G217 (= G217), M218 (= M218), T241 (= T241), N242 (≠ D242), H256 (≠ I254)
Query Sequence
>Echvi_0304 FitnessBrowser__Cola:Echvi_0304
MKKPASATDYLEQITAAVTYISEQHPFIPEVGIILGTGLGQLIDKIEVEASLSYKDIPCF
PISTVETHAGELVLGTLHGVPIVAMKGRFHYYEGYDMKAVTFPVRVMRQLGIKQLIVSNA
AGGLDPAFEVGDVMLINDHIDLFPENPLRGKNLDDFGVRFPDMSEPYDQGWLEAAVRVAK
QEGIKVHQGVYAGVQGPNLETKAEYRYLRTIGADAVGMSTVPEVIVARQMELPVLAMSAI
TDLCSPGKVKKISIQEVIAAAAIAEPKMTRIIASLIRQWSDH
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory