SitesBLAST
Comparing Echvi_0343 Echvi_0343 Enoyl-CoA hydratase/carnithine racemase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
31% identity, 98% coverage: 3:255/258 of query aligns to 4:253/259 of 5zaiC
- active site: A65 (= A64), F70 (≠ I69), S82 (= S84), R86 (≠ K88), G110 (= G112), E113 (≠ G115), P132 (≠ T134), E133 (= E135), I138 (≠ F140), P140 (= P142), G141 (≠ A143), A226 (≠ E228), F236 (≠ R238)
- binding coenzyme a: K24 (= K23), L25 (≠ R24), A63 (≠ S62), G64 (= G63), A65 (= A64), D66 (= D65), I67 (≠ L66), P132 (≠ T134), R166 (≠ L167), F248 (= F250), K251 (= K253)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
33% identity, 99% coverage: 3:258/258 of query aligns to 3:253/256 of 3h81A
- active site: A64 (= A64), M69 (= M72), T79 (≠ L81), F83 (≠ K88), G107 (= G112), E110 (≠ G115), P129 (≠ T134), E130 (= E135), V135 (≠ F140), P137 (= P142), G138 (≠ A143), L223 (≠ E228), F233 (≠ R238)
- binding calcium ion: F233 (≠ R238), Q238 (≠ C243)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
33% identity, 99% coverage: 3:258/258 of query aligns to 4:254/255 of 3q0jC
- active site: A65 (= A64), M70 (= M72), T80 (≠ L81), F84 (≠ K88), G108 (= G112), E111 (≠ G115), P130 (≠ T134), E131 (= E135), V136 (≠ F140), P138 (= P142), G139 (≠ A143), L224 (≠ E228), F234 (≠ R238)
- binding acetoacetyl-coenzyme a: Q23 (≠ E22), A24 (≠ K23), L25 (≠ R24), A27 (= A26), A63 (≠ S62), G64 (= G63), A65 (= A64), D66 (= D65), I67 (= I69), K68 (≠ E70), M70 (= M72), F84 (≠ K88), G107 (≠ A111), G108 (= G112), E111 (≠ G115), P130 (≠ T134), E131 (= E135), P138 (= P142), G139 (≠ A143), M140 (= M144)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
33% identity, 99% coverage: 3:258/258 of query aligns to 4:254/255 of 3q0gC
- active site: A65 (= A64), M70 (= M72), T80 (≠ L81), F84 (≠ K88), G108 (= G112), E111 (≠ G115), P130 (≠ T134), E131 (= E135), V136 (≠ F140), P138 (= P142), G139 (≠ A143), L224 (≠ E228), F234 (≠ R238)
- binding coenzyme a: L25 (≠ R24), A63 (≠ S62), I67 (= I69), K68 (≠ E70), Y104 (≠ H108), P130 (≠ T134), E131 (= E135), L134 (≠ I138)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
30% identity, 99% coverage: 3:258/258 of query aligns to 3:251/254 of 2dubA
- active site: A67 (= A64), M72 (= M72), S82 (≠ A82), G105 (= G112), E108 (≠ G115), P127 (≠ T134), E128 (= E135), T133 (≠ F140), P135 (= P142), G136 (vs. gap), K221 (≠ E228), F231 (≠ R238)
- binding octanoyl-coenzyme a: K25 (≠ E22), A26 (≠ K23), L27 (≠ R24), A29 (= A26), A65 (≠ S62), A67 (= A64), D68 (= D65), I69 (= I69), K70 (≠ E70), G105 (= G112), E108 (≠ G115), P127 (≠ T134), E128 (= E135), G136 (vs. gap), A137 (= A143)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
31% identity, 99% coverage: 3:258/258 of query aligns to 4:255/258 of 1mj3A
- active site: A68 (= A64), M73 (= M72), S83 (≠ A82), L85 (= L87), G109 (= G112), E112 (≠ G115), P131 (≠ T134), E132 (= E135), T137 (≠ F140), P139 (= P142), G140 (vs. gap), K225 (≠ E228), F235 (≠ R238)
- binding hexanoyl-coenzyme a: K26 (≠ E22), A27 (≠ K23), L28 (≠ R24), A30 (= A26), A66 (≠ S62), G67 (= G63), A68 (= A64), D69 (= D65), I70 (= I69), G109 (= G112), P131 (≠ T134), E132 (= E135), L135 (≠ I138), G140 (vs. gap)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
31% identity, 99% coverage: 3:258/258 of query aligns to 3:249/250 of 3q0gD
- active site: A64 (= A64), M69 (= M72), T75 (≠ D78), F79 (≠ A82), G103 (= G112), E106 (≠ G115), P125 (≠ T134), E126 (= E135), V131 (≠ F140), P133 (= P142), G134 (≠ A143), L219 (≠ E228), F229 (≠ R238)
- binding Butyryl Coenzyme A: F225 (≠ N234), F241 (= F250)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
31% identity, 99% coverage: 3:258/258 of query aligns to 4:257/260 of 1dubA
- active site: A68 (= A64), M73 (= M72), S83 (= S84), L87 (≠ K88), G111 (= G112), E114 (≠ G115), P133 (≠ T134), E134 (= E135), T139 (≠ F140), P141 (= P142), G142 (vs. gap), K227 (≠ E228), F237 (≠ R238)
- binding acetoacetyl-coenzyme a: K26 (≠ E22), A27 (≠ K23), L28 (≠ R24), A30 (= A26), A66 (≠ S62), A68 (= A64), D69 (= D65), I70 (= I69), Y107 (≠ H108), G110 (≠ A111), G111 (= G112), E114 (≠ G115), P133 (≠ T134), E134 (= E135), L137 (≠ I138), G142 (vs. gap), F233 (≠ N234), F249 (= F250)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
31% identity, 99% coverage: 3:258/258 of query aligns to 2:255/258 of 1ey3A
- active site: A66 (= A64), M71 (= M72), S81 (= S84), L85 (≠ K88), G109 (= G112), E112 (≠ G115), P131 (≠ T134), E132 (= E135), T137 (≠ F140), P139 (= P142), G140 (vs. gap), K225 (≠ E228), F235 (≠ R238)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ E22), L26 (≠ R24), A28 (= A26), A64 (≠ S62), G65 (= G63), A66 (= A64), D67 (= D65), I68 (= I69), L85 (≠ K88), W88 (≠ F91), G109 (= G112), P131 (≠ T134), L135 (≠ I138), G140 (vs. gap)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
31% identity, 99% coverage: 3:258/258 of query aligns to 34:287/290 of P14604
- E144 (≠ G115) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E135) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
31% identity, 99% coverage: 3:258/258 of query aligns to 4:257/260 of 2hw5C
- active site: A68 (= A64), M73 (= M72), S83 (= S84), L87 (≠ K88), G111 (= G112), E114 (≠ G115), P133 (≠ T134), E134 (= E135), T139 (≠ F140), P141 (= P142), G142 (vs. gap), K227 (≠ E228), F237 (≠ R238)
- binding crotonyl coenzyme a: K26 (≠ E22), A27 (≠ K23), L28 (≠ R24), A30 (= A26), K62 (= K58), I70 (= I69), F109 (≠ L110)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
32% identity, 97% coverage: 6:254/258 of query aligns to 8:254/261 of 5jbxB
- active site: A67 (= A64), R72 (≠ I69), L84 (≠ S84), R88 (≠ K88), G112 (= G112), E115 (≠ G115), T134 (= T134), E135 (= E135), I140 (≠ F140), P142 (= P142), G143 (≠ A143), A228 (≠ E228), L238 (≠ R238)
- binding coenzyme a: S24 (≠ E22), R25 (≠ K23), R26 (= R24), A28 (= A26), A65 (≠ S62), D68 (= D65), L69 (= L66), K70 (≠ Q67), L110 (= L110), G111 (≠ A111), T134 (= T134), E135 (= E135), L138 (≠ I138), R168 (≠ L167)
3p85A Crystal structure enoyl-coa hydratase from mycobacterium avium (see paper)
33% identity, 77% coverage: 12:210/258 of query aligns to 10:191/224 of 3p85A
- active site: L62 (≠ A64), L67 (= L87), P68 (≠ K88), G92 (= G112), E95 (≠ G115), T114 (= T134), H115 (≠ E135), L120 (≠ F140), P122 (= P142), T123 (≠ A143)
- binding calcium ion: D43 (≠ I45), D45 (≠ T47)
Sites not aligning to the query:
Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
32% identity, 94% coverage: 16:258/258 of query aligns to 24:265/270 of Q4WF54
Sites not aligning to the query:
- 268:270 PTS1-type peroxisomal targeting signal
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
31% identity, 96% coverage: 8:254/258 of query aligns to 5:238/245 of 6slaAAA
- active site: Q61 (≠ A64), L68 (≠ R71), N72 (= N75), A96 (≠ G112), S99 (≠ G115), A118 (≠ T134), F119 (≠ E135), L124 (≠ F140), P126 (= P142), N127 (≠ A143), A212 (≠ E228), G222 (≠ R238)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (≠ R24), A59 (≠ S62), Q61 (≠ A64), D62 (= D65), L63 (= L66), L68 (≠ R71), Y71 (≠ D74), A94 (≠ L110), G95 (≠ A111), A96 (≠ G112), F119 (≠ E135), I122 (= I138), L124 (≠ F140), N127 (≠ A143), F234 (= F250), K237 (= K253)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
32% identity, 93% coverage: 16:254/258 of query aligns to 16:250/257 of 6slbAAA
- active site: Q64 (≠ A64), F69 (= F77), L80 (≠ K88), N84 (≠ L92), A108 (≠ G112), S111 (≠ G115), A130 (≠ T134), F131 (≠ E135), L136 (≠ F140), P138 (= P142), D139 (≠ A143), A224 (≠ E228), G234 (≠ R238)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ K58), A62 (≠ S62), Q64 (≠ A64), D65 (= D65), L66 (= L66), Y76 (≠ S84), A108 (≠ G112), F131 (≠ E135), D139 (≠ A143)
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
29% identity, 96% coverage: 8:254/258 of query aligns to 13:259/266 of O53561
- K135 (= K130) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 130:137, 38% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ R137) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
7xwvA Feruloyl-coa hydratase/lyase complexed with vanillin and coenzyme a (see paper)
27% identity, 90% coverage: 2:233/258 of query aligns to 4:236/244 of 7xwvA
- binding coenzyme a: T24 (≠ E22), K25 (= K23), R26 (= R24), A64 (≠ S62), G65 (= G63), M66 (≠ A64), D67 (= D65), L68 (= L66), W111 (≠ H108), F113 (≠ L110), G114 (≠ A111), G115 (= G112), S137 (≠ T134)
- binding 4-hydroxy-3-methoxybenzaldehyde: M66 (≠ A64), Y71 (≠ I69), F72 (≠ E70), E138 (= E135), G146 (≠ A143), G147 (≠ M144)
7xwtB Crystal structure of feruoyl-coa hydratase/lyase complexed with coa from sphingomonas paucimobilis (see paper)
27% identity, 90% coverage: 2:233/258 of query aligns to 5:237/277 of 7xwtB
- binding acetyl coenzyme *a: T25 (≠ E22), K26 (= K23), R27 (= R24), A29 (= A26), A65 (≠ S62), G66 (= G63), M67 (≠ A64), D68 (= D65), L69 (= L66), F73 (≠ E70), F114 (≠ L110), G116 (= G112), S138 (≠ T134), W142 (≠ I138)
6p5uE Structure of an enoyl-coa hydratase/aldolase isolated from a lignin- degrading consortium (see paper)
29% identity, 91% coverage: 5:240/258 of query aligns to 10:242/246 of 6p5uE
- active site: M67 (≠ A64), Y72 (≠ I69), D77 (= D74), R89 (≠ D83), A93 (≠ L87), G117 (= G112), T120 (≠ G115), E140 (= E135), I145 (≠ F140), P147 (= P142), A148 (= A143), A236 (= A231)
- binding coenzyme a: D25 (≠ E22), K26 (= K23), R27 (= R24), A29 (= A26), A65 (≠ S62), M67 (≠ A64), D68 (= D65), L69 (= L66), W113 (≠ H108), F115 (≠ L110), S139 (≠ T134), W143 (≠ I138)
Sites not aligning to the query:
Query Sequence
>Echvi_0343 Echvi_0343 Enoyl-CoA hydratase/carnithine racemase
MEEHVKFEQKGRIGYVILSRPEKRNALNAAMVSGLQAALDRCEAIETVKVVIIKAEGKVF
CSGADLQDIERMQDNTFDENLADSEGLKKLFLRLYTFPKVVIAQVQGHALAGGCGLVSVC
DFAYAVPTAKLGYTEVRIGFVPAMVLVFLVRKLGEGKAKELLLTGELLVAPAAKEIGLIQ
EVFEPAALDGAVQEIAERLISQNSGESMALTKKMIAAVQDLPLEEALEYAARMNAKARET
EDCKRGIAAFLGKKKIEW
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory