SitesBLAST

P24232 Flavohemoprotein; Flavohemoglobin; HMP; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD; EC 1.14.12.17 from Escherichia coli (strain K12) (see 2 papers)
28% identity, 63% coverage: 27:258/370 of query aligns to 157:390/396 of P24232

query
sites
P24232

V

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Sites not aligning to the query:

29 mutation Y->E,H: 15 to 35-fold reduction in NO dioxygenase activity.; Y→F: 30-fold reduction in NO dioxygenase activity, and 80-fold increase in the O(2) dissociation rate constant.
95 active site, Charge relay system
135 active site, Charge relay system

2eixA The structure of physarum polycephalum cytochrome b5 reductase (see paper)
25% identity, 60% coverage: 22:243/370 of query aligns to 16:233/243 of 2eixA

query
sites
2eixA

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active site: H47 (≠ F53), Y63 (= Y69), T64 (≠ S70), C217 (= C230)
binding flavin-adenine dinucleotide: R61 (= R67), P62 (≠ S68), Y63 (= Y69), T64 (≠ S70), I78 (≠ T84), Y82 (≠ I88), K84 (≠ G90), G85 (= G91), M87 (≠ V93), S88 (= S94), T126 (≠ S135), T129 (= T138), F195 (≠ R204), M221 (≠ G234), A225 (vs. gap)

5ylyB Crystal structure of the cytochrome b5 reductase domain of ulva prolifera nitrate reductase (see paper)
29% identity, 48% coverage: 61:237/370 of query aligns to 71:248/269 of 5ylyB

query
sites
5ylyB

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binding flavin-adenine dinucleotide: V82 (≠ C72), V83 (≠ T73), Y86 (= Y76), F94 (≠ R87), G97 (= G90), G98 (= G91), K99 (≠ V92), F100 (≠ V93), S101 (= S94), T149 (≠ S135), T152 (= T138)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 46, 65, 66, 67, 68

A0A286R227 Nitrate reductase [NADH]; NR; EC 1.7.1.1 from Ulva prolifera (Green seaweed) (Enteromorpha prolifera) (see paper)
29% identity, 48% coverage: 61:237/370 of query aligns to 665:842/863 of A0A286R227

query
sites
A0A286R227

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VVKIY 676:680 (≠ CTSPY 72:76) binding
F688 (≠ R87) binding
KFS 693:695 (≠ VVS 92:94) binding
T746 (= T138) binding
C835 (= C230) mutation to A: Loss of enzyme activity to 21% of that of the wild-type, but increased affinity for NADH.

Sites not aligning to the query:

640 H→A: Minimal effect in enzyme activity and a minor change in affinity for NADH.
659 R→A: Loss of enzyme activity to 13% of that of the wild-type and decreased affinity for NADH.
659:662 binding
662 T→A: Loss of enzyme activity to 18% of that of the wild-type and decreased affinity for NADH.

4eh1A Crystal structure of the flavohem-like-fad/NAD binding domain of nitric oxide dioxygenase from vibrio cholerae o1 biovar el tor
31% identity, 58% coverage: 46:258/370 of query aligns to 32:235/237 of 4eh1A

query
sites
4eh1A

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binding flavin-adenine dinucleotide: Y39 (≠ F53), R55 (= R67), Q56 (≠ S68), Y57 (= Y69), S58 (= S70), S71 (≠ T84), V72 (= V85), E75 (≠ I88), N81 (vs. gap), G83 (= G91), L84 (≠ V92), V85 (= V93), S86 (= S94), T127 (= T138), E233 (= E256), F235 (= F258)

5h57A Ferredoxin iii from maize root (see paper)
47% identity, 20% coverage: 289:362/370 of query aligns to 11:84/97 of 5h57A

query
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5h57A

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binding fe2/s2 (inorganic) cluster: Y38 (= Y316), C40 (= C318), R41 (≠ Q319), G43 (= G321), C45 (= C323), C48 (= C326), C78 (= C356)

Q9DCN2 NADH-cytochrome b5 reductase 3; B5R; Cytochrome b5 reductase; Diaphorase-1; EC 1.6.2.2 from Mus musculus (Mouse) (see paper)
29% identity, 59% coverage: 20:237/370 of query aligns to 45:281/301 of Q9DCN2

query
sites
Q9DCN2

L

L

S

R

L

L

K

I

V

D

R

K

E

E

V

V

V

I

R

-

E

-

T

S

P

P

D

D

A

T

V

R

T

R

I

F

Y

R

F

F

E

A

Q

L

P

P

E

S

P

P

-

Q

-

H

Y

I

L

L

E

G

Y

L

K

P

P

I

G

G

Q

Q

F

H

L

I

T

Y

L

L

I

S

L

T

D

R

I

I

N

D

G

G

K

N

E

L

E

V

R

I

R

R

S

P

Y

Y

S

T

L

P

C

V

T

S

S

S

P

D

-

D

-

K

-

G

-

F

-

V

-

D

-

L

-

V

-

K

-

V

-

Y

Y

F

V

K

D

D

P

T

H

H

P

P

G

-

I

-

T

-

V

-

K

K

R

F

I

P

A

A

G

G

V

K

V

M

S

S

N

Q

Y

Y

L

L

N

-

E

E

S

N

I

M

K

K

P

I

G

G

K

D

T

T

I

I

E

E

V

F

M

R

K

G

P

P

M

N

G

G

H

L

F

L

T

V

-

Y

-

Q

-

G

-

K

-

G

-

K

-

F

A

A

D

I

F

R

H

A

S

D

K

K

N

K

K

S

N

N

H

P

F

V

I

V

-

R

-

T

-

V

-

K

-

S

-

V

-

G

M

M

I

I

A

A

G

G

S

T

G

G

I

I

T

T

P

P

I

M

M

L

G

Q

I

V

A

I

K

R

S

A

V

V

L

L

I

K

N

D

E

P

P

N

E

D

S

H

K

T

V

V

T

C

-

Y

L

L

I

L

Y

F

C

A

N

N

R

Q

S

S

E

E

E

K

Q

D

V

I

I

L

F

L

D

R

Q

P

A

E

I

L

Q

E

K

E

L

L

V

R

N

N

E

E

Q

H

K

S

R

A

R

R

F

F

E

K

V

L

V

W

H

Y

T

T

L

V

S

D

R

K

P

A

S

P

G

D

D

A

W

W

S

D

G

Y

L

S

Q

Q

G

G

R

F

L

V

D

N

E

E

Q

E

M

M

I

I

E

R

Q

D

V

H

L

L

E

P

E

-

K

-

H

-

F

-

A

T

A

P

A

G

D

E

K

E

E

P

L

L

Y

I

Y

L

L

M

C

C

G

G

P

P

E

P

G

P

L

M

M

I

E

Q

Sites not aligning to the query:

2 G→A: Decreased levels in mitochondrion and reduced activity of mitochondrial respiratory complex I.

3w2eA Crystal structure of oxidation intermediate (20 min) of nadh- cytochrome b5 reductase from pig liver (see paper)
28% identity, 59% coverage: 20:237/370 of query aligns to 15:251/271 of 3w2eA

query
sites
3w2eA

L

L

S

R

L

L

K

I

V

D

R

K

E

E

V

V

R

N

E

H

T

D

P

T

D

R

A

R

V

F

T

R

I

F

Y

A

F

L

E

P

Q

S

P

P

E

E

P

H

Y

I

L

L

E

G

Y

L

K

P

P

V

G

G

Q

Q

F
x
H

L

I

T

Y

L

L

I

S

L

A

D

R

I

I

N

D

G

G

K

N

E

L

E

V

R

I

R
|
R

S
x
P

Y
|
Y

S
x
T

L

P

C

V

T

S

S

S

P

D

-

D

-

K

-

G

-

F

-

V

-

D

-

L

-
x
V

-

I

-
x
K

-

V

-
x
Y

Y

F

V

K

D

D

P

T

H
|
H

P

P

G

-

I

-

T

-

V

-

K

K

R
x
F

I

P

A

A

G
|
G

V
x
K

V
x
M

S
|
S

N

Q

Y

Y

L

L

N

-

E

E

S

S

I

M

K

K

P

I

G

G

K

D

T

T

I

I

E

E

V

F

M

R

K

G

P

P

M

N

G

G

H

L

F

L

T

V

-

Y

-

Q

-

G

-

K

-

G

-

K

-

F

A

A

D

I

F

R

H

P

S

D

K

K

N

K

K

S

N

S

H

P

F

V

I

I

-

K

-

T

-

V

-

K

-

S

-

V

-

G

M

M

I

I

A

A

G

G

G
|
G

S
x
T

G
|
G

I

I

T
|
T

P

P

I

M

M

L

G

Q

I

V

A

I

K

R

S

A

V

I

L

M

I

K

N

D

E

P

P

D

E

D

S

H

K

T

V

V

T

C

-

H

L

L

I

L

Y

F

C

A

N
|
N

R
x
Q

S

T

E

E

E

K

Q

D

V

I

I

L

F

L

D

R

Q

P

A

E

I

L

Q

E

K

E

L

L

V

R

N

N

E

E

Q

H

K

S

R

A

R

R

F

F

E

K

V

L

V

W

H

Y

T

T

L

V

S
x
D

R

R

P

A

S

P

G

E

D

A

W

W

S

D

G

Y

L

S

Q

Q

G

G

R
x
F

L

V

D

N

E

E

Q

E

M

M

I

I

E

R

Q

D

V

-

L

-

E

-

K

-

H

H

F

L

A

P

D

E

K

E

E

E

-

P

L

L

Y

V

Y

L

L

M

C
|
C

G
|
G

P
|
P

E

P

G
x
P

L
x
M

M

I

E

Q

active site: H48 (≠ F53), Y64 (= Y69), T65 (≠ S70), C244 (= C230)
binding flavin-adenine dinucleotide: R62 (= R67), P63 (≠ S68), Y64 (= Y69), T65 (≠ S70), V79 (vs. gap), Y83 (vs. gap), H88 (= H80), F91 (≠ R87), G94 (= G90), G95 (= G91), K96 (≠ V92), M97 (≠ V93), S98 (= S94), T152 (≠ S135), T155 (= T138)
binding nicotinamide-adenine-dinucleotide: K81 (vs. gap), Y83 (vs. gap), G151 (= G134), T152 (≠ S135), G153 (= G136), N180 (= N162), Q181 (≠ R163), D210 (≠ S192), F222 (≠ R204), C244 (= C230), G245 (= G231), P246 (= P232), P248 (≠ G234), M249 (≠ L235)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 271

5gv7A Structure of nadh-cytochrome b5 reductase refined with the multipolar atomic model at 0.80 a (see paper)
28% identity, 59% coverage: 20:237/370 of query aligns to 16:252/272 of 5gv7A

query
sites
5gv7A

L

L

S

R

L

L

K

I

V

D

R

K

E

E

V

V

R

N

E

H

T

D

P

T

D

R

A

R

V

F

T

R

I

F

Y

A

F

L

E

P

Q

S

P

P

E

E

P

H

Y

I

L

L

E

G

Y

L

K

P

P

V

G

G

Q

Q

F
x
H

L

I

T

Y

L

L

I

S

L

A

D

R

I

I

N

D

G

G

K

N

E

L

E

V

R

I

R
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R

S
x
P

Y
|
Y

S
x
T

L

P

C

V

T

S

S

S

P

D

-

D

-

K

-

G

-

F

-

V

-

D

-

L

-
x
V

-

I

-

K

-

V

-
x
Y

Y

F

V

K

D

D

P

T

H
|
H

P

P

G

-

I

-

T

-

V

-

K

K

R
x
F

I

P

A

A

G
|
G

V
x
K

V
x
M

S
|
S

N

Q

Y

Y

L

L

N

-

E

E

S

S

I

M

K

K

P

I

G

G

K

D

T

T

I

I

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M

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K

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P

P

M

N

G

G

H

L

F

L

T

V

-

Y

-

Q

-

G

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K

-

G

-

K

-

F

A

A

D

I

F

R

H

P

S

D

K

K

N

K

K

S

N

S

H

P

F

V

I

I

-

K

-

T

-

V

-

K

-

S

-

V

-

G

M

M

I

I

A

A

G

G

S
x
T

G

G

I

I

T
|
T

P
|
P

I

M

M

L

G

Q

I

V

A

I

K

R

S

A

V

I

L

M

I

K

N

D

E

P

P

D

E

D

S

H

K

T

V

V

T

C

-

H

L

L

I

L

Y

F

C

A

N

N

R

Q

S

T

E

E

E

K

Q

D

V

I

I

L

F

L

D

R

Q

P

A

E

I

L

Q

E

K

E

L

L

V

R

N

N

E

E

Q

H

K

S

R

A

R

R

F

F

E

K

V

L

V

W

H

Y

T

T

L

V

S

D

R

R

P

A

S

P

G

E

D

A

W

W

S

D

G

Y

L

S

Q

Q

G

G

R

F

L

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D

N

E

E

Q

E

M

M

I

I

E

R

Q

D

V

-

L

-

E

-

K

-

H

H

F

L

A

P

D

E

K

E

E

E

-

P

L

L

Y

V

Y

L

L

M

C
|
C

G

G

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P

E

P

G

P

L

M

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E

Q

active site: H49 (≠ F53), Y65 (= Y69), T66 (≠ S70), C245 (= C230)
binding flavin-adenine dinucleotide: R63 (= R67), P64 (≠ S68), Y65 (= Y69), T66 (≠ S70), V80 (vs. gap), Y84 (vs. gap), H89 (= H80), F92 (≠ R87), G95 (= G90), G96 (= G91), K97 (≠ V92), M98 (≠ V93), S99 (= S94), T153 (≠ S135), T156 (= T138), P157 (= P139)

P83686 NADH-cytochrome b5 reductase 3; B5R; Cytochrome b5 reductase; Diaphorase-1; EC 1.6.2.2 from Sus scrofa (Pig) (see 4 papers)
28% identity, 59% coverage: 20:237/370 of query aligns to 16:252/272 of P83686

query
sites
P83686

L

L

S

R

L

L

K

I

V

D

R

K

E

E

V

V

R

N

E

H

T

D

P

T

D

R

A

R

V

F

T

R

I

F

Y

A

F

L

E

P

Q

S

P

P

E

E

P

H

Y

I

L

L

E

G

Y

L

K

P

P

V

G

G

Q

Q

F
x
H

L

I

T

Y

L

L

I

S

L

A

D

R

I

I

N

D

G

G

K

N

E

L

E

V

R

I

R
|
R

S
x
P

Y
|
Y

S
x
T

L

P

C

V

T

S

S

S

P

D

-

D

-

K

-

G

-

F

-

V

-

D

-

L

-

V

-

I

-

K

-

V

-

Y

Y

F

V

K

D

D

P

T

H

H

P

P

G

-

I

-

T

-

V

-

K

K

R

F

I

P

A

A

G

G

V
x
K

V
x
M

S
|
S

N

Q

Y

Y

L

L

N

-

E

E

S

S

I

M

K

K

P

I

G

G

K

D

T

T

I

I

E

E

V

F

M

R

K

G

P

P

M

N

G

G

H

L

F

L

T

V

-

Y

-

Q

-

G

-

K

-

G

-

K

-

F

A

A

D

I

F

R

H

P

S

D

K

K

N

K

K

S

N

S

H

P

F

V

I

I

-

K

-

T

-

V

-

K

-

S

-

V

-

G

M

M

I

I

A

A

G

G

S

T

G

G

I

I

T

T

P

P

I

M

M

L

G

Q

I

V

A

I

K

R

S

A

V

I

L

M

I

K

N

D

E

P

P

D

E

D

S

H

K

T

V

V

T

C

-

H

L

L

I

L

Y

F

C

A

N

N

R

Q

S

T

E

E

E

K

Q

D

V

I

I

L

F

L

D

R

Q

P

A

E

I

L

Q

E

K

E

L

L

V

R

N

N

E

E

Q

H

K

S

R

A

R

R

F

F

E

K

V

L

V

W

H

Y

T

T

L

V

S

D

R

R

P

A

S

P

G

E

D

A

W

W

S

D

G

Y

L

S

Q

Q

G

G

R

F

L

V

D

N

E

E

Q

E

M

M

I

I

E

R

Q

D

V

-

L

-

E

-

K

-

H

H

F

L

A

P

D

E

K

E

E

E

-

P

L

L

Y

V

Y

L

L

M

C

C

G

G

P

P

E

P

G

P

L

M

M

I

E

Q

H49 (≠ F53) mutation to A: Similar properties to wild-type.; mutation to E: Increase and decrease in Km values for Fe(II)-[cytochrome b5 and NADH respectively.; mutation to K: Decrease and increase in Km values for Fe(II)-[cytochrome b5 and NADH respectively.; mutation to Y: Similar properties to wild-type.
R63 (= R67) binding ; mutation R->A,Q: Increased Km values for NADH and increased dissociation constant for NAD(+).; mutation to K: Km values for NADH and Fe(II)-[cytochrome b5 similar to that of wild-type.
P64 (≠ S68) binding
Y65 (= Y69) binding ; mutation Y->A,F: Protein destabilized, release of FAD accelerated, and kcat values decreased.
T66 (≠ S70) mutation to A: Similar properties to wild-type.; mutation to S: Similar properties to wild-type.; mutation to V: 10% of wild-type kcat values.
K97 (≠ V92) binding ; mutation to A: Little effect on absorption or CD spectra.; mutation to R: Little effect on absorption or CD spectra.
M98 (≠ V93) binding
S99 (= S94) binding ; mutation S->A,V: Increased Km values for NADH and increased dissociation constant for NAD(+).; mutation to T: Km values for NADH and Fe(II)-[cytochrome b5 similar to that of wild-type.

Sites not aligning to the query:

272 F→FG: 10% of wild-type kcat values.; mutation Missing: 54% of wild-type kcat values.

P0A3C7 Ferredoxin-1; Ferredoxin I from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) (see paper)
45% identity, 20% coverage: 289:362/370 of query aligns to 13:86/99 of P0A3C7

query
sites
P0A3C7

E

E

G

G

E

T

E

K

H

H

T

E

F

I

E

E

V

V

P

P

P

D

G

D

K

E

T

Y

I

I

L

L

E

D

A

A

G

A

L

E

D

E

E

Q

G

G

Y

Y

D

D

M

L

P

P

Y

F

S

S

C

C

Q
x
R

S

A

G

G

L

A

C

C

T

S

A
x
T

C

C

R

A

G

G

R

K

L

L

S

V

S

S

G

G

E

T

V

V

K

D

M

Q

E

S

Q

D

D

Q

A

S

G
x
F

L

L

S

D

D
|
D

G
x
D

E

Q

K

I

K

E

D

A

G

G

Y

Y

I

V

L

L

C

T

C

C

V

V

S

A

Y

Y

P

P

K

T

S

S

R43 (≠ Q319) mutation R->A,E,H: No effect on electron transfer.
T49 (≠ A325) mutation to A: No effect on electron transfer.
F66 (≠ G342) mutation F->A,I: 1000-fold decrease in electron transfer.
D69 (= D345) mutation to K: Small effect on electron transfer.
D70 (≠ G346) mutation to K: Small effect on electron transfer.

Sites not aligning to the query:

95 E→K: 20000-fold decrease in electron transfer.

1ewyC Anabaena pcc7119 ferredoxin:ferredoxin-NADP+-reductase complex (see paper)
45% identity, 20% coverage: 289:362/370 of query aligns to 12:85/98 of 1ewyC

query
sites
1ewyC

E

E

G

G

E

T

E

K

H

H

T

E

F

I

E

E

V

V

P

P

P

D

G

D

K

E

T

Y

I

I

L

L

E

D

A

A

G

A

L

E

D

E

E

Q

G

G

Y

Y

D

D

M

L

P

P

Y
x
F

S
|
S

C
|
C

Q
x
R

S

A

G
|
G

L
x
A

C
|
C

T

S

A

T

C
|
C

R

A

G

G

R

K

L

L

S

V

S

S

G

G

E

T

V

V

K

D

M

Q

E

S

Q

D

D

Q

A
x
S

G

F

L

L

S

D

D

D

G

D

E

Q

K

I

K

E

D

A

G

G

Y

Y

I

V

L
|
L

C

T

C
|
C

V

V

S

A

Y

Y

P

P

K

T

S

S

binding flavin-adenine dinucleotide: A45 (≠ L322), S64 (≠ A341)
binding fe2/s2 (inorganic) cluster: F39 (≠ Y316), S40 (= S317), C41 (= C318), R42 (≠ Q319), G44 (= G321), A45 (≠ L322), C46 (= C323), C49 (= C326), L77 (= L354), C79 (= C356)

Query Sequence

>Echvi_0363 FitnessBrowser__Cola:Echvi_0363
MIFNLFKKHKGEKKRGSQYLSLKVREVVRETPDAVTIYFEQPEPYLEYKPGQFLTLILDI
NGKEERRSYSLCTSPYVDPHPGITVKRIAGGVVSNYLNESIKPGKTIEVMKPMGHFTADF
HSKNKNHFIMIAGGSGITPIMGIAKSVLINEPESKVTLIYCNRSEEQVIFDQAIQKLVNE
QKRRFEVVHTLSRPSGDWSGLQGRLDEQMIEQVLEEKHFAAADKELYYLCGPEGLMEASV
EALLKLDVPHENIHRESFYTSSSEEAEKEAETDAADQPALTRSVTIDLEGEEHTFEVPPG
KTILEAGLDEGYDMPYSCQSGLCTACRGRLSSGEVKMEQDAGLSDGEKKDGYILCCVSYP
KSGDVKVDIG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory