SitesBLAST
Comparing Echvi_0643 FitnessBrowser__Cola:Echvi_0643 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1j0bA Crystal structure analysis of the acc deaminase homologue complexed with inhibitor (see paper)
32% identity, 90% coverage: 17:294/309 of query aligns to 26:313/325 of 1j0bA
- active site: K54 (= K44), Y256 (= Y235), Y282 (= Y261)
- binding n-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-y-lmethyl]-1-amino-cyclopropanecarboxylic acid: N53 (= N43), K54 (= K44), S81 (= S71), N82 (= N72), H83 (= H73), A189 (≠ I175), G190 (= G176), S191 (≠ T177), G192 (= G178), G193 (= G179), T194 (= T180), Y282 (= Y261), T308 (≠ S289), G309 (= G290), G310 (= G291)
1j0aA Crystal structure analysis of the acc deaminase homologue (see paper)
32% identity, 90% coverage: 17:294/309 of query aligns to 26:313/325 of 1j0aA
- active site: K54 (= K44), Y256 (= Y235), Y282 (= Y261)
- binding pyridoxal-5'-phosphate: N53 (= N43), K54 (= K44), K57 (= K47), N82 (= N72), G190 (= G176), S191 (≠ T177), G192 (= G178), G193 (= G179), T194 (= T180), Y282 (= Y261), T308 (≠ S289), G309 (= G290), G310 (= G291)
4d8uH Crystal structure of d-cysteine desulfhydrase from salmonella typhimurium at 3.3 a in monoclinic space group with 8 subunits in the asymmetric unit (see paper)
27% identity, 89% coverage: 17:291/309 of query aligns to 26:320/331 of 4d8uH
4d9fA D-cysteine desulfhydrase from salmonella typhimurium complexed with d- cycloserine (dcs) (see paper)
27% identity, 89% coverage: 17:291/309 of query aligns to 23:317/328 of 4d9fA
- active site: K51 (= K44), Y261 (= Y235), Y287 (= Y261)
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: N50 (= N43), K51 (= K44), K54 (= K47), N79 (= N72), G194 (= G176), S195 (≠ T177), A196 (≠ G178), T198 (= T180), Y287 (= Y261), T315 (≠ S289), G316 (= G290), G317 (= G291)
4d9eA D-cysteine desulfhydrase from salmonella typhimurium complexed with l- cycloserine (lcs) (see paper)
27% identity, 89% coverage: 17:291/309 of query aligns to 23:317/328 of 4d9eA
- active site: K51 (= K44), Y261 (= Y235), Y287 (= Y261)
- binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: N50 (= N43), K51 (= K44), N79 (= N72), G194 (= G176), S195 (≠ T177), A196 (≠ G178), T198 (= T180), Y287 (= Y261), T315 (≠ S289), G316 (= G290), G317 (= G291)
4d9bA Pyridoxamine 5' phosphate (pmp) bound form of salmonella typhimurium d-cysteine desulfhydrase obtained after co-crystallization with d- cycloserine (see paper)
27% identity, 89% coverage: 17:291/309 of query aligns to 23:317/328 of 4d9bA
- active site: K51 (= K44), Y261 (= Y235), Y287 (= Y261)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: N50 (= N43), K51 (= K44), N79 (= N72), G194 (= G176), S195 (≠ T177), A196 (≠ G178), T198 (= T180), Y287 (= Y261), T315 (≠ S289), G316 (= G290), G317 (= G291)
4d99A Salmonella typhimurium d-cysteine desulfhydrase with l-ser bound non- covalently at the active site (see paper)
27% identity, 89% coverage: 17:291/309 of query aligns to 23:317/328 of 4d99A
4d97A Salmonella typhimurium d-cysteine desulfhydrase with d-ser bound at active site (see paper)
27% identity, 89% coverage: 17:291/309 of query aligns to 23:317/328 of 4d97A
4d96A D-cysteine desulfhydrase from salmonella typhimurium complexed with 1- amino-1-carboxycyclopropane (acc) (see paper)
27% identity, 89% coverage: 17:291/309 of query aligns to 23:317/328 of 4d96A
- active site: K51 (= K44), Y261 (= Y235), Y287 (= Y261)
- binding n-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-y-lmethyl]-1-amino-cyclopropanecarboxylic acid: N50 (= N43), K51 (= K44), S78 (= S71), N79 (= N72), H80 (= H73), G194 (= G176), S195 (≠ T177), A196 (≠ G178), T198 (= T180), Y287 (= Y261), T315 (≠ S289), G316 (= G290), G317 (= G291)
4d8wA Salmonella typhimurium d-cysteine desulfhydrase soaked with d-cys shows pyruvate bound 4 a away from active site (see paper)
27% identity, 89% coverage: 17:291/309 of query aligns to 23:317/328 of 4d8wA
Q5PWZ8 1-aminocyclopropane-1-carboxylate deaminase; ACC deaminase; ACCD; EC 3.5.99.7 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
25% identity, 89% coverage: 26:299/309 of query aligns to 32:332/338 of Q5PWZ8
- G44 (≠ P38) mutation to D: Loss of activity.
1tzmA Crystal structure of acc deaminase complexed with substrate analog b- chloro-d-alanine (see paper)
24% identity, 89% coverage: 26:299/309 of query aligns to 32:325/331 of 1tzmA
- active site: K51 (= K44), Y261 (= Y235), Y287 (= Y261)
- binding 3-chloro-D-alanine: G74 (= G67), S78 (= S71), N79 (= N72), Q80 (≠ H73), W102 (≠ G90), A153 (≠ E146), G154 (= G147), Y287 (= Y261)
- binding amino-acrylate: K51 (= K44), S78 (= S71), Q80 (≠ H73), Y287 (= Y261)
- binding pyridoxal-5'-phosphate: N50 (= N43), K51 (= K44), K54 (= K47), N79 (= N72), C189 (= C173), S190 (= S174), V191 (≠ I175), T192 (= T177), G193 (= G178), T195 (= T180), Y287 (= Y261), E288 (≠ T262), L315 (≠ S289), G316 (= G290), G317 (= G291)
1tzkA Crystal structure of 1-aminocyclopropane-1-carboxylate-deaminase complexed with alpha-keto-butyrate (see paper)
24% identity, 89% coverage: 26:299/309 of query aligns to 32:325/331 of 1tzkA
- active site: K51 (= K44), Y261 (= Y235), Y287 (= Y261)
- binding 2-ketobutyric acid: K51 (= K44), G74 (= G67), S78 (= S71), N79 (= N72), Q80 (≠ H73), A153 (≠ E146), G154 (= G147), Y287 (= Y261)
- binding pyridoxal-5'-phosphate: N50 (= N43), K51 (= K44), K54 (= K47), N79 (= N72), C189 (= C173), S190 (= S174), V191 (≠ I175), T192 (= T177), G193 (= G178), T195 (= T180), Y287 (= Y261), E288 (≠ T262), L315 (≠ S289), G316 (= G290), G317 (= G291)
1tzjA Crystal structure of 1-aminocyclopropane-1-carboxylate deaminase complexed with d-vinyl glycine (see paper)
24% identity, 89% coverage: 26:299/309 of query aligns to 32:325/331 of 1tzjA
- active site: K51 (= K44), Y261 (= Y235), Y287 (= Y261)
- binding d-vinylglycine: G74 (= G67), S78 (= S71), N79 (= N72), Q80 (≠ H73), A153 (≠ E146), G154 (= G147), Y287 (= Y261)
- binding pyridoxal-5'-phosphate: N50 (= N43), K51 (= K44), K54 (= K47), N79 (= N72), C189 (= C173), S190 (= S174), V191 (≠ I175), T192 (= T177), G193 (= G178), T195 (= T180), Y287 (= Y261), E288 (≠ T262), L315 (≠ S289), G316 (= G290), G317 (= G291)
1tz2A Crystal structure of 1-aminocyclopropane-1-carboyxlate deaminase complexed with acc (see paper)
24% identity, 89% coverage: 26:299/309 of query aligns to 32:325/331 of 1tz2A
- active site: K51 (= K44), Y261 (= Y235), Y287 (= Y261)
- binding 1-aminocyclopropanecarboxylic acid: K51 (= K44), S78 (= S71), Y287 (= Y261)
- binding pyridoxal-5'-phosphate: N50 (= N43), K51 (= K44), K54 (= K47), N79 (= N72), C189 (= C173), S190 (= S174), V191 (≠ I175), T192 (= T177), G193 (= G178), T195 (= T180), Y287 (= Y261), E288 (≠ T262), L315 (≠ S289), G316 (= G290), G317 (= G291)
1rqxC Crystal structure of acc deaminase complexed with inhibitor (see paper)
24% identity, 89% coverage: 26:299/309 of query aligns to 32:332/338 of 1rqxC
- active site: K51 (= K44), Y268 (= Y235), Y294 (= Y261)
- binding 1-aminocyclopropylphosphonate: K51 (= K44), S78 (= S71), N79 (= N72), Q80 (≠ H73), T199 (= T177), Y294 (= Y261)
- binding pyridoxal-5'-phosphate: N50 (= N43), K51 (= K44), K54 (= K47), N79 (= N72), S197 (= S174), V198 (≠ I175), T199 (= T177), G200 (= G178), T202 (= T180), Y294 (= Y261), E295 (≠ T262), L322 (≠ S289), G323 (= G290), G324 (= G291)
1f2dA 1-aminocyclopropane-1-carboxylate deaminase (see paper)
23% identity, 90% coverage: 26:302/309 of query aligns to 32:336/341 of 1f2dA
- active site: K51 (= K44), Y269 (= Y235), Y295 (= Y261)
- binding pyridoxal-5'-phosphate: N50 (= N43), K51 (= K44), K54 (= K47), N79 (= N72), C200 (= C173), T202 (= T177), G203 (= G178), S204 (≠ G179), T205 (= T180), Y295 (= Y261), E296 (≠ T262), L323 (≠ S289), G324 (= G290), G325 (= G291)
Q7M523 1-aminocyclopropane-1-carboxylate deaminase; ACC deaminase; ACCD; EC 3.5.99.7 from Cyberlindnera saturnus (Yeast) (Williopsis saturnus) (see paper)
23% identity, 90% coverage: 26:302/309 of query aligns to 32:336/341 of Q7M523
Sites not aligning to the query:
- 1 modified: N-acetylserine
1j0eA Acc deaminase mutant reacton intermediate (see paper)
23% identity, 90% coverage: 26:302/309 of query aligns to 32:336/341 of 1j0eA
- binding 1-aminocyclopropanecarboxylic acid: S78 (= S71), N79 (= N72), Q80 (≠ H73), F295 (≠ Y261)
- binding pyridoxal-5'-phosphate: N50 (= N43), K51 (= K44), K54 (= K47), N79 (= N72), C199 (= C172), T202 (= T177), G203 (= G178), T205 (= T180), F295 (≠ Y261), E296 (≠ T262)
1j0dA Acc deaminase mutant complexed with acc (see paper)
23% identity, 90% coverage: 26:302/309 of query aligns to 32:336/341 of 1j0dA
- binding n-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-y-lmethyl]-1-amino-cyclopropanecarboxylic acid: S78 (= S71), N79 (= N72), Q80 (≠ H73), A163 (≠ E146), G164 (= G147), C200 (= C173), V201 (≠ S174), T202 (= T177), G203 (= G178), S204 (≠ G179), T205 (= T180), Y295 (= Y261), E296 (≠ T262)
Query Sequence
>Echvi_0643 FitnessBrowser__Cola:Echvi_0643
MLISVLETKNHITQEVFLPEIKEMGVRLFVKRLDKIHPLASGNKFYKLKYNLEAAKQSGN
STVLTFGGAFSNHILATAASAEAAGLKSIGIIRGECPAEPNPTLKQARANGMTLSFMDRT
TYRKKHEKEIIDRLQEEFGNFYLIPEGGTNALAIKGTAEILTDEDQTMDFICCSIGTGGT
IAGLIKSAMPHQKVLGFSALKGEFIHQEIANLLQAHEIPSQNQYALFSTYHFGGYAKHKP
ELIAFIKKFKQATGIPLDPVYTGKLFYGVMDNIKSSFFPPGSHILLIHSGGLQGIQGFNQ
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory