SitesBLAST

R175 (≠ W174) mutation to E: Does not exhibit NADH kinase activity in addition to NAD kinase activity.; mutation to G: Exhibits NADH kinase activity in addition to NAD kinase activity. Reduces the Vmax of the NAD kinase activity.; mutation to H: Exhibits NADH kinase activity in addition to NAD kinase activity.; mutation to I: Does not exhibit NADH kinase activity in addition to NAD kinase activity.; mutation to K: Does not exhibit NADH kinase activity in addition to NAD kinase activity.; mutation to Q: Exhibits NADH kinase activity in addition to NAD kinase activity.; mutation to T: Exhibits NADH kinase activity in addition to NAD kinase activity.

P9WHV7 NAD kinase; ATP-dependent NAD kinase; Poly(P)-dependent NAD kinase; PPNK; EC 2.7.1.23 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
31% identity, 76% coverage: 63:282/291 of query aligns to 75:296/307 of P9WHV7

query
sites
P9WHV7

G

G

M

C

D

E

F

L

V

V

I

L

S

V

V

L

G

G

D
|
D

G

G

T

T

L

F

L

L

D

R

T

A

V

A

S

E

L

L

V

A

G

R

E

N

Y

A

E

S

V

I

P

P

I

V

V

L

G

G

I

V

N

N

T

L

G

G

R

R

M

I

G

G

F

F

L

L

A

A

T

E

I

A

A

E

K

A

E

E

D

A

V

I

E

D

K

A

A

V

V

L

Q

E

V

H

L

V

F

V

D

A

G

Q

D

D

F

Y

T

R

I

V

Q

E

D

D

R

R

I

L

L

T

I

L

N

D

L

V

E

V

A

V

D

R

Q

Q

-

G

K

R

L

I

F

V

N

N

G

R

V

-

P

G

Y

W

G

A

L

L

N
|
N

E
|
E

F

V

T

S

I

L

H

E

K

K

R

G

D

P

T

R

S

L

S

G

M

V

I

L

I

G

V

V

H

V

T

V

Y

E

I

I

D

D

G

G

E

R

Y

P

L

V

N

S

S

A

Y

F

W

G

A

C

D

D

G
|
G

L

V

I
x
L

V

V

A

S

T
|
T

P
|
P

T
|
T

G
|
G

S
|
S

T
|
T

G
x
A

Y
|
Y

S
x
A

L
x
F

S
|
S

C

A

G
|
G

P

P

L

V

I

L

S

W

P

P

S

D

A

L

K

E

N

A

F

I

V

L

I

V

T

V

P

P

V

N

S

N

P

A

H

H

N

A

L

L

N

F

V

G

R

R

P

P

M

M

I

V

V

T

S

S

D

P

E

E

S

A

E

T

I

I

T

A

F

I

S

E

I

I

E

E

G

A

R

D

S

G

K

H

K

D

F

A

L

L

I

V

S

F

L

C

D

D

S

G

-

R

R

R

S

E

T

M

A

L

V

I

D

P

A

A

S

G

V

S

K

R

L

L

K

E

V

V

K

T

R

R

E

C

K

V

F

T

V

S

A

V

R

K

L

W

V

A

K

R

F

L

H

D

D

S

Y

A

S

P

F

F

F

T

D

D

T

R

L

L

R

V

K

R

K

K

D85 (= D73) mutation to A: Abolishes catalytic activity.
N159 (= N146) mutation to A: Abolishes catalytic activity.
NE 159:160 (= NE 146:147) binding
E160 (= E147) mutation to A: Abolishes catalytic activity.
G190 (= G177) mutation to A: Abolishes catalytic activity.
L192 (≠ I179) mutation to A: Abolishes catalytic activity.
T195 (= T182) mutation to A: It promotes stronger allosteric interactions.
P196 (= P183) mutation to A: Abolishes catalytic activity.
T197 (= T184) binding ; mutation to A: Abolishes catalytic activity.
G198 (= G185) mutation to A: Abolishes catalytic activity.
S199 (= S186) mutation to A: Lower catalytic efficiency. A perturbation of the allosteric interactions is observed when NAD is used as substrate.
T200 (= T187) mutation to A: Abolishes catalytic activity.
TAYAFS 200:205 (≠ TGYSLS 187:192) binding
Y202 (= Y189) mutation to A: Abolishes catalytic activity.
G207 (= G194) mutation to A: Abolishes catalytic activity.
G208 (= G195) mutation to A: Possesses 30% of the activity compared to the wild-type enzyme. While mutant affects the catalytic efficiency, it does not alter the binding affinity for ATP and poly(P). It causes a decrease in the affinity for NAD and alters the allosteric interactions mediated by the dinucleotide, both in the presence of poly(P) and ATP.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1y3iA Crystal structure of mycobacterium tuberculosis NAD kinase-NAD complex (see paper)
31% identity, 73% coverage: 71:282/291 of query aligns to 12:225/231 of 1y3iA

query
sites
1y3iA

G

G

D
|
D

G
|
G

T

T

L

F

L

L

D

R

T

A

V

A

S

E

L

L

V

A

G

R

E

N

Y

A

E

S

V

I

P

P

I

V

V

L

G

G

I

V

N

N

T

L

G

G

R
|
R

M

I

G

G

F
|
F

L
|
L

A

A

T

E

I

A

A

E

K

A

E

E

D

A

V

I

E

D

K

A

A

V

V

L

Q

E

V

H

L

V

F

V

D

A

G

Q

D

D

F

Y

T

R

I

V

Q

E

D

D

R

R

I

L

L

T

I

L

N

D

L

V

E

V

A

V

D

R

Q

Q

-

G

K

R

L

I

F

V

N

N

G

R

V

-

P

G

Y

W

G

A

L

L

N
|
N

E
|
E

F

V

T

S

I

L

H

E

K

K

R

G

D

P

T

R

S

L

S

G

M

V

I

L

I

G

V

V

H

V

T

V

Y

E

I

I

D

D

G

G

E

R

Y

P

L

V

N

S

S

A

Y

F

W

G

A

C

D

D

G

G

L

V

I

L

V

V

A

S

T

T

P

P

T

T

G

G

S

S

T
|
T

G
x
A

Y
|
Y

S

A

L

F

S
|
S

C

A

G

G

P

P

L

V

I

L

S

W

P

P

S

D

A

L

K

E

N

A

F

I

V

L

I

V

T

V

P

P

V

N

S

N

P

A

H

H

N

A

L

L

N

F

V

G

R

R

P

P

M

M

I

V

V

T

S

S

D

P

E

E

S

A

E

T

I

I

T

A

F

I

S

E

I

I

E

E

G

A

R

D

S

G

K

H

K

D

F

A

L

L

I

V

S

F

L

C

D

D

S

G

-

R

R

R

S

E

T

M

A

L

V

I

D

P

A

A

S

G

V

S

K

R

L

L

K

E

V

V

K

T

R

R

E

C

K

V

F

T

V

S

A

V

R

K

L

W

V

A

K

R

F

L

H

D

D

S

Y

A

S

P

F

F

F

T

D

D

T

R

L

L

R

V

K

R

K

K

binding nicotinamide-adenine-dinucleotide: D14 (= D73), G15 (= G74), R38 (= R97), F41 (= F100), L42 (= L101), N88 (= N146), E89 (= E147), T129 (= T187), A130 (≠ G188), Y131 (= Y189), S134 (= S192)

3afoA Crystal structure of yeast nadh kinase complexed with nadh
33% identity, 58% coverage: 65:232/291 of query aligns to 112:282/360 of 3afoA

query
sites
3afoA

D

D

F

L

V

L

I

V

S

T

V

L

G

G

D
|
D

G
|
G

T

T

L

I

L
|
L

D

H

T

G

V

V

S

S

L

M

V

F

G

G

E

N

Y

T

E

Q

V

V

P

P

-

P

I

V

V

L

G

A

I

F

N

A

T

L

G

G

R

T

M

L

G

G

F
|
F

L

L

A

S

T

P

I

F

A

D

K

F

E

K

D

E

V

H

E

K

K

K

A

V

V

F

Q

Q

V

E

L

V

F

I

D

S

G

S

D

R

F

A

T

K

I

C

Q

L

D

H

R

R

I

T

L

R

I

L

N

E

L

C

E

H

A

L

D

K

Q

K

K

K

L

D

F

S

N

N

G

S

-

S

-

I

V

V

P

T

Y

H

G

A

L

M

N
|
N

E
x
D

F

I

T

F

I

L

H

H

K

R

R

G

D

N

T

S

S

P

S

H

M

L

I

T

I

N

V

L

H

D

T

I

Y

F

I

I

D

D

G

G

E

E

Y

F

L

L

N

T

S

R

Y

T

W

T

A

A

D

D

G

G

L

V

I

A

V

L

A

A

T

T

P

P

T

T

G

G

S

S

T
|
T

G
x
A

Y
|
Y

S

S

L

L

S
|
S

C

A

G

G

P

S

L

I

I

V

S

S

P

P

S

L

A

V

K

P

N

A

F

I

V

L

I

M

T

T

P

P

V

I

S

C

P

P

H

R

N

S

L

L

N

S

V

F

R

R

P

P

M

L

I

I

V

L

S

P

D

H

E

S

S

S

E

H

I

I

T

R

F

I

S

K

I

I

binding 1,4-dihydronicotinamide adenine dinucleotide: D120 (= D73), G121 (= G74), L124 (= L77), F148 (= F100), N196 (= N146), D197 (≠ E147), T237 (= T187), A238 (≠ G188), Y239 (= Y189), S242 (= S192)

Sites not aligning to the query:

binding 1,4-dihydronicotinamide adenine dinucleotide: 300, 301

1z0zA Crystal structure of a NAD kinase from archaeoglobus fulgidus in complex with NAD (see paper)
33% identity, 73% coverage: 59:269/291 of query aligns to 35:233/249 of 1z0zA

query
sites
1z0zA

K

E

E

E

L

L

A

E

G

N

M

F

D

D

F

F

V

I

I

V

S

S

V

V

G

G

D

D

G

G

T

T

L

I

L

L

D

R

T

I

V

L

S

Q

L

K

V

L

G

K

E

R

Y

C

E

P

V

-

P

P

I

I

V

F

G

G

I

I

N

N

T

T

G

G

R

R

M

V

G

G

F

L

L

L

A

T

T

H

I

A

A

S

K

P

E

E

D

N

V

F

E

E

-

V

-

E

-

L

-

K

K

K

A

A

V

V

Q

E

V

-

L

-

F

-

D

-

G

-

D

K

F

F

T
x
E

I

V

Q

E

D

R

R

F

I

P

L

R

I

V

N

S

L
x
C

E

S

A

A

D

M

Q

P

K

D

L

V

F

L

N

-

G

-

V

-

P

-

Y

-

G

A

L

L

N
|
N

E
|
E

F

I

T

A

I

V

H

L

K

S

R

R

D

K

T

P

S

A

S

K

M
|
M

I

I

I

D

V

V

H

A

T

L

Y

R

I

V

D

D

G

G

E

V

Y

E

L

V

N

D

S

R

Y

I

W
x
R

A

C

D
|
D

G

G

L

F

I

I

V

V

A

A

T

T

P

Q

T

I

G

G

S

S

T
|
T

G

G

Y
|
Y

S

A

L

F

S
|
S

C

A

G

G

P

P

L

V

I

V

S

E

P

P

S

Y

A

L

K

E

N

C

F

F

V

I

I

L

T

I

P

P

V

I

S

A

P

P

H
x
F

N

R

L

F

N

G

V

W

R

K

P

P

M

Y

I

V

V

V

S

S

D

M

E

E

S

R

E

K

I

-

T

-

F

-

S

-

I

I

E

E

G

V

R

I

S

A

K

E

K

K

F

A

L

I

I

V

S

V

L

A

D
|
D

S
x
G

R

Q

S

K

T

S

A

-

V

V

D

D

A

F

S

D

V

G

K

E

L

I

K

T

V

I

K

E

R

K

E

S

K

E

F

F

V

P

A

A

R

V

L

F

V

F

K

K

active site: E96 (≠ T122), C105 (≠ L131)
binding nicotinamide-adenine-dinucleotide: N115 (= N146), E116 (= E147), M127 (= M158), R143 (≠ W174), D145 (= D176), T156 (= T187), Y158 (= Y189), S161 (= S192), F182 (≠ H213), D209 (= D244), G210 (≠ S245)

1z0sA Crystal structure of an NAD kinase from archaeoglobus fulgidus in complex with atp (see paper)
33% identity, 73% coverage: 59:269/291 of query aligns to 35:233/249 of 1z0sA

query
sites
1z0sA

K

E

E

E

L

L

A

E

G

N

M

F

D

D

F

F

V

I

I

V

S

S

V

V

G

G

G
|
G

D

D

G
|
G

T
|
T

L

I

L

L

D
x
R

T

I

V

L

S

Q

L

K

V

L

G

K

E

R

Y

C

E

P

V

-

P

P

I

I

V

F

G

G

I

I

N

N

T

T

G

G

R
|
R

M

V

G

G

F

L

L

L

A

T

T

H

I

A

A

S

K

P

E

E

D

N

V

F

E

E

-

V

-

E

-

L

-

K

K

K

A

A

V

V

Q

E

V

-

L

-

F

-

D

-

G

-

D

K

F

F

T
x
E

I

V

Q

E

D

R

R

F

I

P

L

R

I

V

N

S

L
x
C

E

S

A

A

D

M

Q

P

K

D

L

V

F

L

N

-

G

-

V

-

P

-

Y

-

G

A

L

L

N
|
N

E
|
E

F

I

T

A

I

V

H

L

K

S

R

R

D

K

T

P

S
x
A

S
x
K

M
|
M

I

I

I

D

V

V

H

A

T

L

Y

R

I

V

D

D

G

G

E

V

Y

E

L

V

N

D

S

R

Y

I

W

R

A

C

D
|
D

G

G

L

F

I

I

V

V

A

A

T

T

P

Q

T

I

G

G

S

S

T

T

G
|
G

Y
|
Y

S

A

L

F

S
|
S

C

A

G

G

P

P

L

V

I

V

S

E

P

P

S

Y

A

L

K

E

N

C

F

F

V

I

I

L

T

I

P

P

V

I

S
x
A

P

P

H
x
F

N

R

L

F

N

G

V

W

R

K

P

P

M

Y

I

V

V

V

S

S

D

M

E

E

S

R

E

K

I

-

T

-

F

-

S

-

I

I

E

E

G

V

R

I

S

A

K

E

K

K

F

A

L

I

I

V

S

V

L

A

D
|
D

S

G

R

Q

S

K

T

S

A

-

V

V

D

D

A

F

S

D

V

G

K

E

L

I

K

T

V

I

K

E

R

K

E

S

K

E

F

F

V

P

A

A

R

V

L

F

V

F

K

K

active site: E96 (≠ T122), C105 (≠ L131)
binding adenosine-5'-triphosphate: R54 (≠ D78), N115 (= N146), E116 (= E147), A125 (≠ S156), K126 (≠ S157), M127 (= M158), D145 (= D176), G157 (= G188), Y158 (= Y189), S161 (= S192), A180 (≠ S211), F182 (≠ H213), D209 (= D244)
binding pyrophosphate 2-: G48 (= G72), G50 (= G74), T51 (= T75), R54 (≠ D78), R72 (= R97)

Sites not aligning to the query:

binding pyrophosphate 2-: 8

1suwA Crystal structure of a NAD kinase from archaeoglobus fulgidus in complex with its substrate and product: insights into the catalysis of NAD kinase (see paper)
33% identity, 73% coverage: 59:269/291 of query aligns to 35:233/249 of 1suwA

query
sites
1suwA

K

E

E

E

L

L

A

E

G

N

M

F

D

D

F

F

V

I

I

V

S

S

V

V

G

G

G
|
G

D
|
D

G
|
G

T

T

L

I

L

L

D

R

T

I

V

L

S

Q

L

K

V

L

G

K

E

R

Y

C

E

P

V

-

P

P

I

I

V

F

G

G

I

I

N

N

T

T

G

G

R

R

M

V

G

G

F

L

L

L

A

T

T

H

I

A

A

S

K

P

E

E

D

N

V

F

E

E

-

V

-

E

-

L

-

K

K

K

A

A

V

V

Q

E

V

-

L

-

F

-

D

-

G

-

D

K

F

F

T

E

I

V

Q

E

D

R

R

F

I

P

L

R

I

V

N

S

L

C

E

S

A

A

D

M

Q

P

K

D

L

V

F

L

N

-

G

-

V

-

P

-

Y

-

G

A

L

L

N
|
N

E
|
E

F

I

T

A

I

V

H

L

K

S

R

R

D

K

T

P

S
x
A

S

K

M
|
M

I

I

I

D

V

V

H

A

T

L

Y

R

I

V

D

D

G

G

E

V

Y

E

L

V

N

D

S

R

Y

I

W
x
R

A

C

D
|
D

G

G

L

F

I

I

V

V

A

A

T

T

P

Q

T

I

G

G

S

S

T
|
T

G

G

Y
|
Y

S

A

L

F

S
|
S

C

A

G

G

P

P

L

V

I

V

S

E

P

P

S

Y

A

L

K

E

N

C

F

F

V

I

I

L

T

I

P

P

V

I

S

A

P

P

H
x
F

N

R

L

F

N

G

V

W

R

K

P

P

M

Y

I

V

V

V

S

S

D

M

E

E

S

R

E

K

I

-

T

-

F

-

S

-

I

I

E

E

G

V

R

I

S

A

K

E

K

K

F

A

L

I

I

V

S

V

L

A

D
|
D

S
x
G

R

Q

S

K

T

S

A

-

V

V

D

D

A

F

S

D

V

G

K

E

L

I

K

T

V

I

K

E

R

K

E

S

K

E

F

F

V

P

A

A

R

V

L

F

V

F

K

K

binding nadp nicotinamide-adenine-dinucleotide phosphate: G48 (= G72), D49 (= D73), G50 (= G74), N115 (= N146), E116 (= E147), A125 (≠ S156), M127 (= M158), R143 (≠ W174), D145 (= D176), T156 (= T187), Y158 (= Y189), S161 (= S192), F182 (≠ H213), D209 (= D244), G210 (≠ S245)

O30297 NAD kinase; ATP-dependent NAD kinase; EC 2.7.1.23 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
33% identity, 73% coverage: 59:269/291 of query aligns to 35:233/249 of O30297

query
sites
O30297

K

E

E

E

L

L

A

E

G

N

M

F

D

D

F

F

V

I

I

V

S

S

V

V

G

G

D
|
D

G
|
G

T

T

L

I

L

L

D
x
R

T

I

V

L

S

Q

L

K

V

L

G

K

E

R

Y

C

E

P

V

-

P

P

I

I

V

F

G

G

I

I

N

N

T

T

G

G

R

R

M

V

G

G

F

L

L

L

A

T

T

H

I

A

A

S

K

P

E

E

D

N

V

F

E

E

-

V

-

E

-

L

-

K

K

K

A

A

V

V

Q

E

V

-

L

-

F

-

D

-

G

-

D

K

F

F

T

E

I

V

Q

E

D

R

R

F

I

P

L

R

I

V

N

S

L

C

E

S

A

A

D

M

Q

P

K

D

L

V

F

L

N

-

G

-

V

-

P

-

Y

-

G

A

L

L

N
|
N

E
|
E

F

I

T

A

I

V

H

L

K

S

R

R

D

K

T

P

S

A

S
x
K

M

M

I

I

I

D

V

V

H

A

T

L

Y

R

I

V

D

D

G

G

E

V

Y

E

L

V

N

D

S

R

Y

I

W
x
R

A

C

D

D

G

G

L

F

I

I

V

V

A

A

T

T

P

Q

T
x
I

G

G

S

S

T
|
T

G
|
G

Y
|
Y

S
x
A

L
x
F

S
|
S

C

A

G

G

P

P

L

V

I

V

S

E

P

P

S

Y

A

L

K

E

N

C

F

F

V

I

I

L

T

I

P

P

V

I

S

A

P

P

H

F

N

R

L

F

N

G

V

W

R

K

P

P

M

Y

I

V

V

V

S

S

D

M

E

E

S

R

E

K

I

-

T

-

F

-

S

-

I

I

E

E

G

V

R

I

S

A

K

E

K

K

F

A

L

I

I

V

S

V

L

A

D

D

S

G

R
x
Q

S

K

T

S

A

-

V

V

D

D

A

F

S

D

V

G

K

E

L

I

K

T

V

I

K

E

R

K

E

S

K

E

F

F

V

P

A

A

R

V

L

F

V

F

K

K

DG 49:50 (= DG 73:74) binding
R54 (≠ D78) binding
NE 115:116 (= NE 146:147) binding
K126 (≠ S157) binding
R143 (≠ W174) binding
I153 (≠ T184) binding
TGYAFS 156:161 (≠ TGYSLS 187:192) binding
Q211 (≠ R246) binding

O13863 Uncharacterized kinase C1B1.02c; EC 2.7.1.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
27% identity, 76% coverage: 65:286/291 of query aligns to 280:512/537 of O13863

query
sites
O13863

D

D

F

C

V

V

I

I

S

T

V

V

G

G

G

D

D

D

G

S

T

A

L

A

L

L

D

R

T

A

V

S

S

W

L

L

V

F

G

Q

E

D

Y

V

E

V

V

P

P

P

I

V

V

L

G

S

I

F

N

S

T

T

G

A

R

K

M

A

G

G

F

F

L

L

A

S

T

I

I

L

A

P

K

I

E

A

D

E

V

Y

E

T

K

K

A

T

V

L

Q

D

V

L

L

I

F

F

D

H

G

R

D

G

F

F

T

T

I

V

Q

N

D

L

R

R

I

M

L

R

I

F

N

Q

L

C

-

S

-

I

-

M

-

R

-

Y

-

V

-

G

E

E

A

H

D

S

Q

T

K

H

L

I

F

C

N

E

G

G

V

Q

P

Y

Y

S

G

V

L

L

N

N

E

E

F

L

T

L

I

I

H

D

K

R

R

G

D

P

T

N

S

P

S

F

M

M

I

I

I

S

V

L

H

D

T

L

Y

Y

I

V

D

E

G

N

E

E

Y

Y

L

I

N

T

S

T

Y

L

W

Q

A

S

D

D

G

G

L

V

I

C

V

V

A

S

T

T

P

P

T

T

G

G

S

S

T

T

G

A

Y

Y

S

S

L

V

S

A

C

A

G

G

P

S

L

L

I

C

S

H

P

P

S

G

A

I

K

P

N

A

F

I

V

L

I

I

T

S

P

A

V

I

S

C

P

P

H

H

N

S

L

L

N

S

V

F

R

R

P

P

M

I

I

I

V

L

S

P

D

D

E

S

S

M

E

T

I

L

T

-

F

-

S

-

I

-

E

-

G

-

R

-

S

-

K

-

K

R

F

I

L

V

I

V

S

P

L

L

D

D

S

A

R

R

S

S

T

N

A

A

V

W

D

C

A

A

-

F

-

D

-

G

-

H

-

R

-

I

-

E

-

L

-

G

-

L

-

G

-

D

S

Y

V

I

K

S

L

I

K

S

V

A

K

S

R

S

E

F

K

P

F

F

V

P

A

S

R

V

L

I

V

R

K

S

F

K

H

Y

D

S

Y

K

S

D

F

W

F

F

D

D

T

I

L

L

R

R

K

Q

K

T

L

L

N

N

W

W

G

N

Sites not aligning to the query:

72 modified: Phosphoserine

Q9P7K3 Uncharacterized kinase C24B10.02c; EC 2.7.1.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
26% identity, 78% coverage: 65:290/291 of query aligns to 176:411/449 of Q9P7K3

query
sites
Q9P7K3

D

D

F

L

V

A

I

I

S

T

V

I

G

G

G

D

D

N

G

S

T

T

L

L

D

Y

T

T

V

S

S

W

L

L

V

F

G

Q

E

K

Y

I

E

G

V

P

P

P

I

V

V

L

G

S

I

F

N

S

T

D

G

D

R

D

M

V

-

P

G

G

F

F

L

L

A

T

T

H

I

F

A

S

K

L

E

S

D

N

V

Y

E

Q

K

Q

A

H

V

L

Q

Y

V

Q

L

V

F

L

D

T

G

Q

D

N

F

V

T

S

I

L

Q

R

-

F

-

C

D

S

R

R

I

L

L

Q

I

C

N

S

L

F

-

H

-

K

-

Y

-

D

-

E

E

K

A

T

D

K

Q

Q

K

Y

L

S

F

L

N

A

G

S

V

T

P

T

Y

Y

G

S

L

L

N

D

E

E

F

I

T

L

I

I

H

S

K

R

R

G

D

E

T

H

S

P

S

F

M

I

I

S

I

N

V

L

H

N

T

V

Y

Y

I

N

D

N

G

S

E

E

Y

L

L

M

N

T

S

V

Y

V

W

Q

A

A

D

D

G

G

L

L

I

V

V

V

A

A

T

T

P

P

T

T

G

G

S

S

T

T

G

N

Y

I

S

S

L

A

S

N

C

A

G

G

P

S

L

L

I

V

S

H

P

P

S

A

A

L

K

N

N

A

F

I

V

L

I

V

T

T

P

P

V

V

S

C

P

P

H

H

N

T

L

L

N

S

V

F

R

R

P

P

M

I

I

I

V

L

S

P

D

D

E

Y

S

N

E

-

I

-

T

V

F

L

S

N

I

V

E

E

G

-

R

-

S

-

K

-

F

-

L

-

I

I

S

P

L

L

D

D

S

S

R

R

S

S

T

S

A

A

-

F

-

S

V

V

D

D

A

R

S

H

V

E

K

S

L

V

K

E

V

M

K

H

R

R

E

G

K

D

F

Y

V

L

A

S

R

I

L

V

V

T

K

S

F

H

H

Y

D

P

-

F

-

T

-

I

-

Q

-

N

-

P

-

G

Y

Y

S

Q

F

W

F

T

D

K

T

V

L

L

R

E

K

D

K

K

L

F

N

N

W

W

G

N

F

V

D

R

M

E

R

R

Sites not aligning to the query:

420 modified: Phosphoserine

7zzdA Crystal structure of NAD kinase 1 from listeria monocytogenes in complex with a linear di-adenosine derivative (see paper)
28% identity, 63% coverage: 65:247/291 of query aligns to 37:223/262 of 7zzdA

query
sites
7zzdA

D

E

F

I

V

V

I

I

S

S

V

I

G

G

D
|
D

G
|
G

T

T

L

F

L

L

D

S

T

A

V

F

S

H

L

Q

V

Y

G

E

E

E

Y

R

-

L

-

D

E

E

V

I

P

A

I

F

V

I

G

G

I

I

N

H

T

T

G

G

R

H

M

L

G

G

F
|
F

L
x
Y

A

A

T

D

I

W

A

R

K

P

E

A

D

E

V

A

E

D

K

K

A

L

V

V

Q

K

V

L

L

L

F

A

D

K

G

G

D

E

F

Y

T

Q

I

K

Q

V

D

S

R

Y

I

P

L

L

I

L

N

K

L

T

E

T

A

V

D

K

Q

Y

K

G

L

I

F

K

N

E

G

A

V

T

P

Y

Y

L

G

A

L

L

N
|
N

E
|
E

F

S

T

T

I

V

H

-

K

-

R

K

D

S

T

S

S

G

M

P

I

F

I

V

V

V

H

D

T

V

Y

V

I

I

D

N

G

D

E

I

Y

H

L

F

N

E

S

R

Y

F

W

R

A

G

D

D

G

G

L

L

I

C

V

M

A

S

T

T

P

P

T

S

G

G

S

T

T
|
T

G
x
A

Y
|
Y

S

N

L

K

S
|
S

C

L

G

G

P

A

L

L

I

M

S

H

P

P

S

S

A

I

K

E

N

A

F

M

V

Q

I

L

T

T

P

E

V

M

S

A

P

S

H

I

N

N

L

N

N

R

V

V

-

Y

-

R

-

T

-

I

-

G

R

S

P

P

M

L

I

V

V

F

S

P

D

K

E

H

S

H

E

V

I

V

T

S

F

L

S

Q

I

-

E

P

G

V

R

N

S

D

K

K

K

D

F

F

L

Q

I

I

S

S

L

V

D

D

S
x
H

R

L

S

S

binding 2-[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl-[3-[6-azanyl-9-[(2~{R},3~{R},4~{S},5~{R})-5-[(3-azanylpropanoylamino)methyl]-3,4-bis(oxidanyl)oxolan-2-yl]purin-8-yl]prop-2-ynyl]amino]ethanoic acid: D45 (= D73), G46 (= G74), F74 (= F100), Y75 (≠ L101), N120 (= N146), E121 (= E147), T159 (= T187), A160 (≠ G188), Y161 (= Y189), S164 (= S192), H221 (≠ S245)

7zz9A Crystal structure of NAD kinase 1 from listeria monocytogenes in complex with a linear di-adenosine derivative (see paper)
28% identity, 63% coverage: 65:247/291 of query aligns to 37:223/262 of 7zz9A

query
sites
7zz9A

D

E

F

I

V

V

I

I

S

S

V

I

G

G

D
|
D

G
|
G

T

T

L

F

L
|
L

D

S

T

A

V

F

S

H

L

Q

V

Y

G

E

E

E

Y

R

-

L

-

D

E

E

V

I

P

A

I

F

V

I

G

G

I

I

N

H

T

T

G

G

R

H

M

L

G

G

F
|
F

L
x
Y

A

A

T

D

I

W

A

R

K

P

E

A

D

E

V

A

E

D

K

K

A

L

V

V

Q

K

V

L

L

L

F

A

D

K

G

G

D

E

F

Y

T

Q

I

K

Q

V

D

S

R

Y

I

P

L

L

I

L

N

K

L

T

E

T

A

V

D

K

Q

Y

K

G

L

I

F

K

N

E

G

A

V

T

P

Y

Y

L

G

A

L

L

N
|
N

E
|
E

F

S

T

T

I

V

H

-

K

-

R

K

D

S

T

S

S

G

M

P

I

F

I

V

V

V

H

D

T

V

Y

V

I

I

D

N

G

D

E

I

Y

H

L

F

N

E

S

R

Y

F

W

R

A

G

D

D

G

G

L

L

I

C

V

M

A

S

T

T

P

P

T

S

G

G

S

T

T
|
T

G
x
A

Y
|
Y

S

N

L

K

S
|
S

C

L

G

G

P

A

L

L

I

M

S

H

P

P

S

S

A

I

K

E

N

A

F

M

V

Q

I

L

T

T

P

E

V

M

S

A

P

S

H

I

N

N

L

N

N

R

V

V

-

Y

-

R

-

T

-

I

-

G

R

S

P

P

M

L

I

V

V

F

S

P

D

K

E

H

S

H

E

V

I

V

T

S

F

L

S

Q

I

-

E

P

G

V

R

N

S

D

K

K

K

D

F

F

L

Q

I

I

S

S

L

V

D

D

S

H

R

L

S

S

binding 3-[[(2~{R},3~{S},4~{R},5~{R})-5-[8-[3-[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl-(2-azanylethyl)amino]prop-1-ynyl]-6-azanyl-purin-9-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methylamino]-3-oxidanylidene-propanoic acid: D45 (= D73), G46 (= G74), L49 (= L77), F74 (= F100), Y75 (≠ L101), N120 (= N146), E121 (= E147), T159 (= T187), A160 (≠ G188), Y161 (= Y189), S164 (= S192)

6z64A Crystal structure of NAD kinase 1 from listeria monocytogenes in complex with a di-adenosine derivative (see paper)
28% identity, 63% coverage: 65:247/291 of query aligns to 37:223/262 of 6z64A

query
sites
6z64A

D

E

F

I

V

V

I

I

S

S

V

I

G

G

D
|
D

G

G

T

T

L

F

L
|
L

D

S

T

A

V

F

S

H

L

Q

V

Y

G

E

E

E

Y

R

-

L

-

D

E

E

V

I

P

A

I

F

V

I

G

G

I

I

N

H

T

T

G

G

R

H

M

L

G

G

F
|
F

L
x
Y

A

A

T

D

I

W

A

R

K

P

E

A

D

E

V

A

E

D

K

K

A

L

V

V

Q

K

V

L

L

L

F

A

D

K

G

G

D

E

F

Y

T

Q

I

K

Q

V

D

S

R

Y

I

P

L

L

I

L

N

K

L

T

E

T

A

V

D

K

Q

Y

K

G

L

I

F

K

N

E

G

A

V

T

P

Y

Y

L

G

A

L

L

N
|
N

E
|
E

F

S

T

T

I

V

H

-

K

-

R

K

D

S

T

S

S

G

M

P

I

F

I

V

V

V

H

D

T

V

Y

V

I

I

D

N

G

D

E

I

Y

H

L

F

N

E

S

R

Y

F

W

R

A

G

D

D

G

G

L

L

I

C

V

M

A

S

T

T

P

P

T

S

G

G

S

T

T
|
T

G
x
A

Y
|
Y

S

N

L

K

S
|
S

C

L

G

G

P

A

L

L

I

M

S

H

P

P

S

S

A

I

K

E

N

A

F

M

V

Q

I

L

T

T

P

E

V

M

S

A

P

S

H

I

N

N

L

N

N

R

V

V

-

Y

-

R

-

T

-

I

-

G

R

S

P

P

M

L

I

V

V

F

S

P

D

K

E

H

S

H

E

V

I

V

T

S

F

L

S

Q

I

-

E

P

G

V

R

N

S

D

K

K

K

D

F

F

L

Q

I

I

S

S

L

V

D

D

S

H

R

L

S

S

binding (2~{R},3~{R},4~{S},5~{R})-2-(6-aminopurin-9-yl)-5-[[3-[6-azanyl-9-[(2~{R},3~{R},4~{S},5~{R})-5-(hydroxymethyl)-3,4-bis(oxidanyl)oxolan-2-yl]purin-8-yl]prop-2-ynyl-(3-azanylpropyl)amino]methyl]oxolane-3,4-diol: D45 (= D73), L49 (= L77), F74 (= F100), Y75 (≠ L101), N120 (= N146), E121 (= E147), T159 (= T187), A160 (≠ G188), Y161 (= Y189), S164 (= S192)

5ejiA Crystal structure of NAD kinase w78f mutant from listeria monocytogenes in complex with NADP/mn++/ppi
29% identity, 63% coverage: 65:247/291 of query aligns to 37:221/260 of 5ejiA

query
sites
5ejiA

D

E

F

I

V

V

I

I

S

S

V

I

G

G

G
|
G

D
|
D

G
|
G

T
|
T

L

F

L

L

D

S

T

A

V

F

S

H

L

Q

V

Y

G

E

E

E

Y

R

-

L

-

D

E

E

V

I

P

A

I

F

V

I

G

G

I

I

N

H

T

T

G

G

R

H

M
x
L

G
|
G

F
|
F

L
x
Y

A

A

T

D

I

F

A

R

K

P

E

A

D

E

V

A

E

D

K

K

A

L

V

V

Q

K

V

L

L

L

F

A

D

K

G

G

D

E

F

Y

T

Q

I

K

Q

V

D

S

R

Y

I

P

L

L

I

-

N

-

L

L

E

K

A

T

D

T

Q

V

K

K

L

Y

F

G

N

E

G

A

V

T

P

Y

Y

L

G

A

L

L

N
|
N

E
|
E

F

S

T

T

I

V

H

-

K

-

R

K

D

S

T

S

S

G

M

P

I

F

I

V

V

V

H

D

T

V

Y

V

I

I

D

N

G

D

E

I

Y

H

L

F

N

E

S

R

Y

F

W

R

A

G

D

D

G

G

L

L

I

C

V

M

A

S

T

T

P

P

T

S

G

G

S

T

T
|
T

G
x
A

Y
|
Y

S

N

L

K

S
|
S

C

L

G

G

P

A

L

L

I

M

S

H

P

P

S

S

A

I

K

E

N

A

F

M

V

Q

I

L

T

T

P

E

V

M

S

A

P

S

H

I

N

N

L

N

N

R

V

V

-

Y

-

R

-

T

-

I

-

G

R

S

P

P

M

L

I

V

V

F

S

P

D

K

E

H

S

H

E

V

I

V

T

S

F

L

S

Q

I

-

E

P

G

V

R

N

S

D

K

K

K

D

F

F

L

Q

I

I

S

S

L

V

D

D

S

H

R

L

S

S

binding nadp nicotinamide-adenine-dinucleotide phosphate: G44 (= G72), D45 (= D73), L72 (≠ M98), G73 (= G99), F74 (= F100), Y75 (≠ L101), N118 (= N146), E119 (= E147), T157 (= T187), A158 (≠ G188), Y159 (= Y189), S162 (= S192)
binding pyrophosphate 2-: G44 (= G72), D45 (= D73), G46 (= G74), T47 (= T75)

6rbzA Crystal structure of NAD kinase 1 from listeria monocytogenes in complexe with an adenine derivative (see paper)
28% identity, 63% coverage: 65:247/291 of query aligns to 37:223/262 of 6rbzA

query
sites
6rbzA

D

E

F

I

V

V

I

I

S

S

V

I

G

G

D
|
D

G
|
G

T

T

L

F

L

L

D

S

T

A

V

F

S

H

L

Q

V

Y

G

E

E

E

Y

R

-

L

-

D

E

E

V

I

P

A

I

F

V

I

G

G

I

I

N

H

T

T

G

G

R

H

M

L

G

G

F
|
F

L

Y

A

A

T

D

I

W

A

R

K

P

E

A

D

E

V

A

E

D

K

K

A

L

V

V

Q

K

V

L

L

L

F

A

D

K

G

G

D

E

F

Y

T

Q

I

K

Q

V

D

S

R

Y

I

P

L

L

I

L

N

K

L

T

E

T

A

V

D

K

Q

Y

K

G

L

K

F

K

N

E

G

A

V

T

P

Y

Y

L

G

A

L

L

N
|
N

E

E

F

S

T

T

I

V

H

-

K

-

R

K

D

S

T

S

S

G

M

P

I

F

I

V

V

V

H

D

T

V

Y

V

I

I

D

N

G

D

E

I

Y

H

L

F

N

E

S

R

Y

F

W

R

A

G

D

D

G

G

L

L

I

C

V

M

A

S

T

T

P

P

T

S

G

G

S

T

T
|
T

G
x
A

Y

Y

S

N

L

K

S

S

C

L

G

G

P

A

L

L

I

M

S

H

P

P

S

S

A

I

K

E

N

A

F

M

V

Q

I

L

T

T

P

E

V

M

S

A

P

S

H

I

N

N

L

N

N

R

V

V

-

Y

-

R

-

T

-

I

-

G

R

S

P

P

M

L

I

V

V

F

S

P

D

K

E

H

S

H

E

V

I

V

T

S

F

L

S

Q

I

-

E

P

G

V

R

N

S

D

K

K

K

D

F

F

L

Q

I

I

S

S

L

V

D

D

S

H

R

L

S

S

binding 9-(3-azanylpropyl)-8-bromanyl-purin-6-amine: D45 (= D73), G46 (= G74), F74 (= F100), N120 (= N146), T159 (= T187), A160 (≠ G188)

5dhuA Crystal structure of NAD kinase 1 from listeria monocytogenes in complex with a novel inhibitor (see paper)
28% identity, 63% coverage: 65:247/291 of query aligns to 37:224/263 of 5dhuA

query
sites
5dhuA

D

E

F

I

V

V

I

I

S

S

V

I

G

G

D

D

G

G

T

T

L

F

L

L

D

S

T

A

V

F

S

H

L

Q

V

Y

G

E

E

E

Y

R

-

L

-

D

E

E

V

I

P

A

I

F

V

I

G

G

I

I

N

H

T

T

G

G

R

H

M

L

G

G

F

F

L

Y

A

A

T

D

I

W

A

R

K

P

E

A

D

E

V

A

E

D

K

K

A

L

V

V

Q

K

V

L

L

L

F

A

D

K

G

G

D

E

F

Y

T

Q

I

K

Q

V

D

S

R

Y

I

P

L

L

I

L

N

K

L

T

E

T

A

V

D

K

Q

Y

K

G

L

I

F

K

N

K

G

E

V

A

P

T

Y

Y

-

L

G

A

L

L

N

N

E
|
E

F

S

T

T

I

V

H

-

K

-

R

K

D

S

T

S

S

G

S
x
G

M
x
P

I

F

I

V

V

V

H

D

T

V

Y

V

I

I

D

N

G

D

E

I

Y

H

L

F

N

E

S

R

Y

F

W
x
R

A
x
G

D
|
D

G

G

L

L

I

C

V

M

A

S

T

T

P

P

T

S

G

G

S

T

T

T

G

A

Y
|
Y

S

N

L

K

S
|
S

C

L

G

G

P

A

L

L

I

M

S

H

P

P

S

S

A

I

K

E

N

A

F

M

V

Q

I

L

T

T

P

E

V

M

S
x
A

P

S

H

I

N

N

L

N

N

R

V

V

-

Y

-

R

-

T

-

I

-

G

R

S

P

P

M

L

I

V

V

F

S

P

D

K

E

H

S

H

E

V

I

V

T

S

F

L

S

Q

I

-

E

P

G

V

R

N

S

D

K

K

K

D

F

F

L

Q

I

I

S

S

L

V

D

D

S
x
H

R

L

S

S

binding 5'-azido-5'-deoxy-8-[(2-{[2-(1H-indol-3-yl)ethyl]amino}-2-oxoethyl)sulfanyl]adenosine: E122 (= E147), G130 (≠ S157), P131 (≠ M158), R147 (≠ W174), G148 (≠ A175), D149 (= D176), Y162 (= Y189), S165 (= S192), A184 (≠ S211), H222 (≠ S245)

5dhtA Crystal structure of NAD kinase 1 from listeria monocytogenes in complex with a novel inhibitor (see paper)
28% identity, 63% coverage: 65:247/291 of query aligns to 37:224/263 of 5dhtA

query
sites
5dhtA

D

E

F

I

V

V

I

I

S

S

V

I

G

G

D

D

G

G

T

T

L

F

L

L

D

S

T

A

V

F

S

H

L

Q

V

Y

G

E

E

E

Y

R

-

L

-

D

E

E

V

I

P

A

I

F

V

I

G

G

I

I

N

H

T

T

G

G

R

H

M

L

G

G

F

F

L

Y

A

A

T

D

I

W

A

R

K

P

E

A

D

E

V

A

E

D

K

K

A

L

V

V

Q

K

V

L

L

L

F

A

D

K

G

G

D

E

F

Y

T

Q

I

K

Q

V

D

S

R

Y

I

P

L

L

I

L

N

K

L

T

E

T

A

V

D

K

Q

Y

K

G

L

I

F

K

N

K

G

E

V

A

P

T

Y

Y

-

L

G

A

L

L

N
|
N

E
|
E

F

S

T

T

I

V

H

-

K

-

R

K

D

S

T

S

S

G

M
x
P

I

F

I

V

V

V

H

D

T

V

Y

V

I

I

D

N

G

D

E

I

Y

H

L

F

N

E

S

R

Y

F

W

R

A
x
G

D
|
D

G

G

L

L

I

C

V

M

A

S

T

T

P

P

T

S

G

G

S

T

T

T

G
x
A

Y
|
Y

S

N

L

K

S
|
S

C

L

G

G

P

A

L

L

I

M

S

H

P

P

S

S

A

I

K

E

N

A

F

M

V

Q

I

L

T

T

P

E

V

M

S
x
A

P

S

H

I

N

N

L

N

N

R

V

V

-

Y

-

R

-

T

-

I

-

G

R

S

P

P

M

L

I

V

V

F

S

P

D

K

E

H

S

H

E

V

I

V

T

S

F

L

S

Q

I

-

E

P

G

V

R

N

S

D

K

K

K

D

F

F

L

Q

I

I

S

S

L

V

D

D

S

H

R

L

S

S

binding 5'-azido-8-[(2-{[2-(3-bromophenyl)ethyl]amino}-2-oxoethyl)sulfanyl]-5'-deoxyadenosine: N121 (= N146), E122 (= E147), P131 (≠ M158), G148 (≠ A175), D149 (= D176), A161 (≠ G188), Y162 (= Y189), S165 (= S192), A184 (≠ S211)

5dhsA Crystal structure of NAD kinase 1 from listeria monocytogenes in complex with a novel inhibitor (see paper)
28% identity, 63% coverage: 65:247/291 of query aligns to 37:224/263 of 5dhsA

query
sites
5dhsA

D

E

F

I

V

V

I

I

S

S

V

I

G

G

D

D

G

G

T

T

L

F

L

L

D

S

T

A

V

F

S

H

L

Q

V

Y

G

E

E

E

Y

R

-

L

-

D

E

E

V

I

P

A

I

F

V

I

G

G

I

I

N

H

T

T

G

G

R

H

M

L

G

G

F

F

L

Y

A

A

T

D

I

W

A

R

K

P

E

A

D

E

V

A

E

D

K

K

A

L

V

V

Q

K

V

L

L

L

F

A

D

K

G

G

D

E

F

Y

T

Q

I

K

Q

V

D

S

R

Y

I

P

L

L

I

L

N

K

L

T

E

T

A

V

D

K

Q

Y

K

G

L

I

F

K

N

K

G

E

V

A

P

T

Y

Y

-

L

G

A

L

L

N
|
N

E
|
E

F

S

T

T

I

V

H

-

K

-

R

K

D

S

T

S

S

G

S
x
G

M
x
P

I

F

I

V

V

V

H

D

T

V

Y

V

I

I

D

N

G

D

E

I

Y

H

L

F

N

E

S

R

Y

F

W
x
R

A
x
G

D
|
D

G

G

L

L

I

C

V

M

A

S

T

T

P

P

T

S

G

G

S

T

T

T

G

A

Y
|
Y

S

N

L

K

S
|
S

C

L

G

G

P

A

L

L

I

M

S

H

P

P

S

S

A

I

K

E

N

A

F

M

V

Q

I

L

T

T

P

E

V

M

S
x
A

P

S

H

I

N

N

L

N

N

R

V

V

-

Y

-

R

-

T

-

I

-

G

R

S

P

P

M

L

I

V

V

F

S

P

D

K

E

H

S

H

E

V

I

V

T

S

F

L

S

Q

I

-

E

P

G

V

R

N

S

D

K

K

K

D

F

F

L

Q

I

I

S

S

L

V

D

D

S
x
H

R

L

S

S

binding 5'-azido-5'-deoxy-8-[(2-{[2-(3-ethynylphenyl)ethyl]amino}-2-oxoethyl)sulfanyl]adenosine: N121 (= N146), E122 (= E147), G130 (≠ S157), P131 (≠ M158), R147 (≠ W174), G148 (≠ A175), D149 (= D176), Y162 (= Y189), S165 (= S192), A184 (≠ S211), H222 (≠ S245)

5dhrA Crystal structure of NAD kinase 1 from listeria monocytogenes in complex with a novel inhibitor (see paper)
28% identity, 63% coverage: 65:247/291 of query aligns to 37:224/263 of 5dhrA

query
sites
5dhrA

D

E

F

I

V

V

I

I

S

S

V

I

G

G

D

D

G

G

T

T

L

F

L

L

D

S

T

A

V

F

S

H

L

Q

V

Y

G

E

E

E

Y

R

-

L

-

D

E

E

V

I

P

A

I

F

V

I

G

G

I

I

N

H

T

T

G

G

R

H

M

L

G

G

F

F

L

Y

A

A

T

D

I

W

A

R

K

P

E

A

D

E

V

A

E

D

K

K

A

L

V

V

Q

K

V

L

L

L

F

A

D

K

G

G

D

E

F

Y

T

Q

I

K

Q

V

D

S

R

Y

I

P

L

L

I

L

N

K

L

T

E

T

A

V

D

K

Q

Y

K

G

L

I

F

K

N

K

G

E

V

A

P

T

Y

Y

-

L

G

A

L

L

N
|
N

E
|
E

F

S

T

T

I

V

H

-

K

-

R

K

D

S

T

S

S

G

S
x
G

M
x
P

I

F

I

V

V

V

H

D

T

V

Y

V

I

I

D

N

G

D

E

I

Y

H

L

F

N

E

S

R

Y

F

W
x
R

A
x
G

D
|
D

G

G

L

L

I

C

V

M

A

S

T

T

P

P

T

S

G

G

S

T

T

T

G

A

Y
|
Y

S

N

L

K

S
|
S

C

L

G

G

P

A

L

L

I

M

S

H

P

P

S

S

A

I

K

E

N

A

F

M

V

Q

I

L

T

T

P

E

V

M

S

A

P

S

H

I

N

N

L

N

N

R

V

V

-

Y

-

R

-

T

-

I

-

G

R

S

P

P

M

L

I

V

V

F

S

P

D

K

E

H

S

H

E

V

I

V

T

S

F

L

S

Q

I

-

E

P

G

V

R

N

S

D

K

K

K

D

F

F

L

Q

I

I

S

S

L

V

D

D

S
x
H

R

L

S

S

binding 5'-azido-8-[(2-{[2-(1H-benzimidazol-2-yl)ethyl]amino}-2-oxoethyl)sulfanyl]-5'-deoxyadenosine: N121 (= N146), E122 (= E147), G130 (≠ S157), P131 (≠ M158), R147 (≠ W174), G148 (≠ A175), D149 (= D176), Y162 (= Y189), S165 (= S192), H222 (≠ S245)

Query Sequence

>Echvi_0656 Echvi_0656 Predicted sugar kinase
MKITIHGISFQKEFVPYIEKLIAVLHGHGTDLFLTETFSKYLKKSGAKATGFTVLGERKE
LAGMDFVISVGGDGTLLDTVSLVGEYEVPIVGINTGRMGFLATIAKEDVEKAVQVLFDGD
FTIQDRILINLEADQKLFNGVPYGLNEFTIHKRDTSSMIIVHTYIDGEYLNSYWADGLIV
ATPTGSTGYSLSCGGPLISPSAKNFVITPVSPHNLNVRPMIVSDESEITFSIEGRSKKFL
ISLDSRSTAVDASVKLKVKREKFVARLVKFHDYSFFDTLRKKLNWGFDMRN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory