SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_0695 FitnessBrowser__Cola:Echvi_0695 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

P09148 Galactose-1-phosphate uridylyltransferase; Gal-1-P uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase; EC 2.7.7.12 from Escherichia coli (strain K12) (see 4 papers)
51% identity, 100% coverage: 1:346/347 of query aligns to 1:344/348 of P09148

query
sites
P09148

M

M

T

T

D

Q

F

F

N

N

F

P

E

V

D

D

H

H

S

P

H

H

R

R

Y

Y

N

N

P

P

F

L

T

T

G

G

D

Q

W

W

L

I

Q

L

V

V

S

S

P

P

H

H

R
|
R

G
x
A

K
|
K

R
|
R

P

P

W

W

Q

Q

G

G

Q

A

E

Q

E

E

D

T

T

P

A

A

E

K

A

Q

Q

V

K

L

P

P

A

A

Y

H

D

D

E

P

K

D

C
|
C

Y

F

L

L

C
|
C

P

A

G

G

N

N

T

V

R

R

I
x
V

N

T

G

G

E

D

K

K

N

N

P

P

D

D

Y

Y

T

T

G

G

A

T

Y

Y

V

V

F

F

Q

T

N
|
N

D
|
D

F

F

G

A

A

A

L

L

T

M

S

S

D

D

I

T

P

P

Q

D

G

A

E

P

M

E

S

S

E

H

G

D

E

P

F

L

F

M

R

R

A

C

K

Q

S

S

E

A

R

R

G

G

I

T

C

S

K

R

V

V

I

I

C

C

F

F

S

S

P

P

R

D

H
|
H

D

S

L

K

T

T

I

L

P

P

E

E

L

L

D

S

V

V

Q

A

A

A

I

L

T

T

K

E

V

I

V

V

E

K

L

T

W

W

K

Q

K

E

E

Q

Y

T

Q

A

E

E

L

L

G

G

G

K

K

T

D

-

F

-

I

Y

N

P

H

W

V

V

Q

Q

I

V

F

F

E

E

N
|
N

K

K

G

G

S

A

V

A

M

M

G
|
G

C
|
C

S
|
S

N

N

P

P

H
|
H

P

P

H
|
H

G

G

Q
|
Q

I

I

W

W

A

A

Q

N

E

S

S

F

I

L

P

P

V

N

E

E

P

A

A

E

K

R

K
x
E

Q

D

V

R

K

L

F

Q

G

K

E

E

Y

Y

Y

F

Q

A

K

E

Y

Q

G

K

R

S

S

P

M

M

V

L

L

V

D

D

Y

Y

V

V

Y

Q

E

R

E

E

L

L

K

A

K

D

G

G

E

S

R

R

I

T

L

V

F

V

E

E

N

T

D

E

Y

H

F

W

V

L

G

A

L

V

V

V

P

P

F

Y

W

W

A

A

V

A

W

W

P

P

F

F

E

E

A

T

M

L

I

L

A

L

P

P

K

K

T

A

H

H

I

V

A

L

S

R

L

I

S

T

E

D

M

L

D

T

A

D

A

A

Q

Q

M

R

E

S

A

D

L

L

A

A

D

L

A

A

Y

L

R

K

Q

K

L

L

A

T

I

S

M

R

Y

Y

D

D

N

N

V

L

F

F

K

Q

V

C

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

I

W

H
|
H

Q

G

A

A

P

P

T

F

D

N

G

G

Q

E

N

E

H

N

P

Q

E

H

W

W

D

Q

L

L

H
|
H

M

A

V
x
H

F

F

Y

Y

P

P

L

L

R

R

S

S

A

A

T

T

V

V

K

R

K
|
K

F
|
F

M

M

V

V

G

G

Y
|
Y

E
|
E

M

M

L

L

A

A

N

E

P

T

Q
|
Q

R

R

D

D

I

L

T

T

A

A

E

E

S

Q

A

A

V

A

K

E

I

R

L

L

K

R

S

A

Q

V

P

S

K

D

E

I

H

H

Y

F

K

R

RAKR 28:31 (≠ RGKR 28:31) binding
C52 (= C52) binding ; mutation to S: Decreases enzyme activity 3000-fold.
C55 (= C55) binding ; mutation to S: Decreases enzyme activity 600-fold.
V61 (≠ I61) binding in other chain
ND 77:78 (= ND 77:78) binding in other chain
H115 (= H115) binding ; mutation to N: Decreases enzyme activity by 98%.
N153 (= N155) binding in other chain
GCS 159:161 (= GCS 161:163) binding in other chain
C160 (= C162) mutation C->S,A: Slight inhibition of enzymatic activity.
S161 (= S163) mutation to A: 7000-fold reduction in specific activity.
H164 (= H166) binding ; mutation to N: Decreases enzyme activity 10000-fold.
H166 (= H168) active site, Tele-UMP-histidine intermediate; mutation to G: Abolishes enzymatic activity.
Q168 (= Q170) binding in other chain
E182 (≠ K184) binding ; mutation to A: Decreases enzyme activity by about 50%. Abolishes iron binding, but has no effect on zinc binding.
H281 (= H283) binding
H296 (= H298) binding
H298 (≠ V300) binding
KF 311:312 (= KF 313:314) binding
YE 316:317 (= YE 318:319) binding
Q323 (= Q325) binding in other chain

1guqA Structure of nucleotidyltransferase complexed with udp-glucose (see paper)
51% identity, 99% coverage: 2:346/347 of query aligns to 1:343/347 of 1guqA

query
sites
1guqA

T

T

D

Q

F

F

N

N

F

P

E

V

D

D

H

H

S

P

H

H

R

R

Y

Y

N

N

P

P

F

L

T

T

G

G

D

Q

W

W

L

I

Q

L

V

V

S

S

P

P

H

H

R
|
R

G

A

K

K

R
|
R

P

P

W
|
W

Q

Q

G

G

Q

A

E

Q

E

E

D

T

T

P

A

A

E

K

A

Q

Q

V

K

L

P

P

A

A

Y

H

D

D

E

P

K

D

C
|
C

Y
x
F

L

L

C
|
C

P

A

G

G

N

N

T

V

R

R

I
x
V

N

T

G

G

E

D

K

K

N

N

P

P

D

D

Y

Y

T

T

G

G

A

T

Y

Y

V

V

F

F

Q

T

N
|
N

D
|
D

F

F

G

A

A

A

L

L

T

M

S

S

D

D

I

T

P

P

Q

D

G

A

E

P

M

E

S

S

E

H

G

D

E

P

F

L

F

M

R

R

A

C

K

Q

S

S

E

A

R

R

G

G

I

T

C

S

K

R

V

V

I

I

C

C

F

F

S

S

P

P

R

D

H
|
H

D

S

L

K

T

T

I

L

P

P

E

E

L

L

D

S

V

V

Q

A

A

A

I

L

T

T

K

E

V

I

V

V

E

K

L

T

W

W

K

Q

K

E

E

Q

Y

T

Q

A

E

E

L

L

G

G

G

K

K

T

D

-

F

-

I

Y

N

P

H

W

V

V

Q

Q

I

V

F
|
F

E
|
E

N
|
N

K
|
K

G

G

S

A

V

A

M

M

G
|
G

C
|
C

S
|
S

N

N

P

P

H
|
H

P

P

H
x
G

G

G

Q
|
Q

I

I

W

W

A

A

Q

N

E

S

S

F

I

L

P

P

V

N

E

E

P

A

A

E

K

R

K
x
E

Q

D

V

R

K

L

F

Q

G

K

E

E

Y

Y

Y

F

Q

A

K

E

Y

Q

G

K

R

S

S

P

M

M

V

L

L

V

D

D

Y

Y

V

V

Y

Q

E

R

E

E

L

L

K

A

K

D

G

G

E

S

R

R

I

T

L

V

F

V

E

E

N

T

D

E

Y

H

F

W

V

L

G

A

L

V

V

V

P

P

F

Y

W

W

A

A

V

A

W

W

P

P

F

F

E

E

A

T

M

L

I

L

A

L

P

P

K

K

T

A

H

H

I

V

A

L

S

R

L

I

S

T

E

D

M

L

D

T

A

D

A

A

Q

Q

M

R

E

S

A

D

L

L

A

A

D

L

A

A

Y

L

R

K

Q

K

L

L

A

T

I

S

M

R

Y

Y

D

D

N

N

V

L

F

F

K

Q

V

C

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

I

W

H
|
H

Q

G

A

A

P

P

T

F

D

N

G

G

Q

E

N

E

H

N

P

Q

E

H

W

W

D

Q

L

L

H
|
H

M

A

V
x
H

F

F

Y

Y

P

P

L

L

R

R

S

S

A

A

T

T

V

V

K

R

K
|
K

F
|
F

M

M

V

V

G

G

Y
|
Y

E
|
E

M

M

L

L

A

A

N

E

P

T

Q

Q

R

R

D

D

I

L

T

T

A

A

E

E

S

Q

A

A

V

A

K

E

I

R

L

L

K

R

S

A

Q

V

P

S

K

D

E

I

H

H

Y

F

K

R

active site: C51 (= C52), C54 (= C55), H114 (= H115), N152 (= N155), S160 (= S163), H163 (= H166), G165 (≠ H168), Q167 (= Q170)
binding fe (iii) ion: E181 (≠ K184), H280 (= H283), H295 (= H298), H297 (≠ V300)
binding potassium ion: E151 (= E154), N152 (= N155), K153 (= K156), G165 (≠ H168)
binding uridine-5'-diphosphate-glucose: R27 (= R28), R30 (= R31), W32 (= W33), F52 (≠ Y53), V60 (≠ I61), N76 (= N77), D77 (= D78), F150 (= F153), N152 (= N155), G158 (= G161), C159 (= C162), S160 (= S163), Q167 (= Q170), K310 (= K313), F311 (= F314), Y315 (= Y318), E316 (= E319)
binding zinc ion: C51 (= C52), C54 (= C55), H114 (= H115), H163 (= H166)

1gupA Structure of nucleotidyltransferase complexed with udp-galactose (see paper)
51% identity, 99% coverage: 2:346/347 of query aligns to 1:343/347 of 1gupA

query
sites
1gupA

T

T

D

Q

F

F

N

N

F

P

E

V

D

D

H

H

S

P

H

H

R

R

Y

Y

N

N

P

P

F

L

T

T

G

G

D

Q

W

W

L

I

Q

L

V

V

S

S

P

P

H

H

R
|
R

G

A

K

K

R
|
R

P

P

W

W

Q

Q

G

G

Q

A

E

Q

E

E

D

T

T

P

A

A

E

K

A

Q

Q

V

K

L

P

P

A

A

Y

H

D

D

E

P

K

D

C
|
C

Y
x
F

L

L

C
|
C

P

A

G

G

N

N

T

V

R
|
R

I
x
V

N

T

G

G

E

D

K

K

N

N

P

P

D

D

Y

Y

T

T

G

G

A

T

Y

Y

V

V

F

F

Q

T

N
|
N

D
|
D

F
|
F

G

A

A

A

L

L

T

M

S

S

D

D

I

T

P

P

Q

D

G

A

E

P

M

E

S

S

E

H

G

D

E

P

F

L

F

M

R

R

A

C

K

Q

S

S

E

A

R

R

G

G

I

T

C

S

K

R

V

V

I

I

C

C

F

F

S

S

P

P

R

D

H
|
H

D

S

L

K

T

T

I

L

P

P

E

E

L

L

D

S

V

V

Q

A

A

A

I

L

T

T

K

E

V

I

V

V

E

K

L

T

W

W

K

Q

K

E

E

Q

Y

T

Q

A

E

E

L

L

G

G

G

K

K

T

D

-

F

-

I

Y

N

P

H

W

V

V

Q

Q

I

V

F
|
F

E

E

N
|
N

K
|
K

G

G

S

A

V

A

M

M

G
|
G

C
|
C

S
|
S

N

N

P

P

H
|
H

P

P

H
x
G

G

G

Q
|
Q

I

I

W
|
W

A

A

Q

N

E

S

S

F

I

L

P

P

V

N

E

E

P

A

A

E

K

R

K
x
E

Q

D

V

R

K

L

F

Q

G

K

E

E

Y

Y

Y

F

Q

A

K

E

Y

Q

G

K

R

S

S

P

M

M

V

L

L

V

D

D

Y

Y

V

V

Y

Q

E

R

E

E

L

L

K

A

K

D

G

G

E

S

R

R

I

T

L

V

F

V

E

E

N

T

D

E

Y

H

F

W

V

L

G

A

L

V

V

V

P

P

F

Y

W

W

A

A

V

A

W

W

P

P

F

F

E

E

A

T

M

L

I

L

A

L

P

P

K

K

T

A

H

H

I

V

A

L

S

R

L

I

S

T

E

D

M

L

D

T

A

D

A

A

Q

Q

M

R

E

S

A

D

L

L

A

A

D

L

A

A

Y

L

R

K

Q

K

L

L

A

T

I

S

M

R

Y

Y

D

D

N

N

V

L

F

F

K

Q

V

C

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

I

W

H
|
H

Q

G

A

A

P

P

T

F

D

N

G

G

Q

E

N

E

H

N

P

Q

E

H

W

W

D

Q

L

L

H
|
H

M

A

V
x
H

F

F

Y

Y

P

P

L

L

R

R

S

S

A

A

T

T

V

V

K

R

K
|
K

F
|
F

M

M

V
|
V

G
|
G

Y

Y

E
|
E

M

M

L

L

A

A

N

E

P

T

Q

Q

R

R

D

D

I

L

T

T

A

A

E

E

S

Q

A

A

V

A

K

E

I

R

L

L

K

R

S

A

Q

V

P

S

K

D

E

I

H

H

Y

F

K

R

active site: C51 (= C52), C54 (= C55), H114 (= H115), N152 (= N155), S160 (= S163), H163 (= H166), G165 (≠ H168), Q167 (= Q170)
binding fe (iii) ion: E181 (≠ K184), H280 (= H283), H295 (= H298), H297 (≠ V300)
binding galactose-uridine-5'-diphosphate: R27 (= R28), R30 (= R31), F52 (≠ Y53), R59 (= R60), V60 (≠ I61), N76 (= N77), D77 (= D78), F78 (= F79), F150 (= F153), N152 (= N155), G158 (= G161), C159 (= C162), S160 (= S163), Q167 (= Q170), W169 (= W172), K310 (= K313), F311 (= F314), V313 (= V316), G314 (= G317), E316 (= E319)
binding potassium ion: N152 (= N155), K153 (= K156), G165 (≠ H168)
binding zinc ion: C51 (= C52), C54 (= C55), H114 (= H115), H163 (= H166)

1hxpA Nucleotide transferase (see paper)
51% identity, 99% coverage: 2:346/347 of query aligns to 1:336/340 of 1hxpA

query
sites
1hxpA

T

T

D

Q

F

F

N

N

F

P

E

V

D

D

H

H

S

P

H

H

R

R

Y

Y

N

N

P

P

F

L

T

T

G

G

D

Q

W

W

L

I

Q

L

V

V

S

S

P

P

H

H

R

R

G

A

K

K

R

R

P

P

W

W

Q

E

G

G

Q

-

E

-

D

-

T

-

A

-

E

-

A

A

Q

V

K

L

P

P

A

A

Y

H

D

D

E

P

K

D

C
|
C

Y
x
F

L

L

C
|
C

P

A

G

G

N

N

T

V

R

R

I
x
V

N

T

G

G

E

D

K

K

N

N

P

P

D

D

Y

Y

T

T

G

G

A

T

Y

Y

V

V

F

F

Q

T

N
|
N

D
|
D

F

F

G

A

A

A

L

L

T

M

S

S

D

D

I

T

P

P

Q

D

G

A

E

P

M

E

S

S

E

H

G

D

E

P

F

L

F

M

R

R

A

C

K

Q

S

S

E

A

R

R

G

G

I

T

C

S

K

R

V

V

I

I

C

C

F

F

S

S

P

P

R

D

H
|
H

D

S

L

K

T

T

I

L

P

P

E

E

L

L

D

S

V

V

Q

A

A

A

I

L

T

T

K

E

V

I

V

V

E

K

L

T

W

W

K

Q

K

E

E

Q

Y

T

Q

A

E

E

L

L

G

G

G

K

K

T

D

-

F

-

I

Y

N

P

H

W

V

V

Q

Q

I

V

F

F

E

E

N
|
N

K

K

G

G

S

A

V

A

M

M

G

G

C
|
C

S
|
S

N

N

P

P

H
|
H

P

P

H
|
H

G

G

Q
|
Q

I

I

W

W

A

A

Q

N

E

S

S

F

I

L

P

P

V

N

E

E

P

A

A

E

K

R

K
x
E

Q

D

V

R

K

L

F

Q

G

K

E

E

Y

Y

Y

F

Q

A

K

E

Y

Q

G

K

R

S

S

P

M

M

V

L

L

V

D

D

Y

Y

V

V

Y

Q

E

R

E

E

L

L

K

A

K

D

G

G

E

S

R

R

I

T

L

V

F

V

E

E

N

T

D

E

Y

H

F

W

V

L

G

A

L

V

V

V

P

P

F

Y

W

W

A

A

V

A

W

W

P

P

F

F

E

E

A

T

M

L

I

L

A

L

P

P

K

K

T

A

H

H

I

V

A

L

S

R

L

I

S

T

E

D

M

L

D

T

A

D

A

A

Q

Q

M

R

E

S

A

D

L

L

A

A

D

L

A

A

Y

L

R

K

Q

K

L

L

A

T

I

S

M

R

Y

Y

D

D

N

N

V

L

F

F

K

Q

V
x
C

S
|
S

F

F

P

P

Y

Y

S

S

A

M

G

G

I

W

H
|
H

Q

G

A

A

P

P

T

F

D

N

G

G

Q

E

N

E

H

N

P

Q

E

H

W

W

D

Q

L

L

H
|
H

M

A

V
x
H

F

F

Y

Y

P

P

L
|
L

L

L

R

R

S

S

A
|
A

T

T

V

V

K

R

K

K

F

F

M

M

V

V

G

G

Y

Y

E

E

M

M

L

L

A

A

N

E

P

T

Q

Q

R

R

D

D

I

L

T

T

A

A

E

E

S

Q

A

A

V

A

K

E

I

R

L

L

K

R

S

A

Q

V

P

S

K

D

E

I

H

H

Y

F

K

R

active site: C44 (= C52), C47 (= C55), H107 (= H115), N145 (= N155), S153 (= S163), H156 (= H166), H158 (= H168), Q160 (= Q170)
binding beta-mercaptoethanol: N145 (= N155), C152 (= C162), Q160 (= Q170), C264 (≠ V274), S265 (= S275), L295 (= L305), A299 (= A309)
binding fe (iii) ion: E174 (≠ K184), H273 (= H283), H288 (= H298), H290 (≠ V300)
binding uridine-5'-monophosphate: F45 (≠ Y53), V53 (≠ I61), N69 (= N77), D70 (= D78)
binding zinc ion: C44 (= C52), C47 (= C55), H107 (= H115), H156 (= H166)

1hxpB Nucleotide transferase (see paper)
49% identity, 99% coverage: 2:346/347 of query aligns to 1:327/329 of 1hxpB

query
sites
1hxpB

T

T

D

Q

F
|
F

N

N

F
x
P

E

V

D

D

H

H

S

P

H
|
H

R

R

Y

Y

N

N

P

P

F

L

T

T

G

G

D

Q

W

W

L

I

Q

L

V

V

S

S

P

P

H

H

R

R

G

-

K

-

R

-

P

-

W

-

Q

-

G

-

Q

-

E

-

D

-

T

-

A

-

E

-

A

-

Q

-

K

L

P

P

A

A

Y

H

D

D

E

P

K

D

C
|
C

Y

F

L

L

C
|
C

P

A

G

G

N

N

T

V

R
|
R

I
x
V

N

T

G

G

E

D

K

K

N

N

P

P

D

D

Y

Y

T

T

G

G

A

T

Y

Y

V

V

F
|
F

Q

T

N
|
N

D
|
D

F

F

G

A

A

A

L

L

T

M

S

S

D

D

I

T

P

P

Q

D

G

A

E

P

M

E

S

S

E

H

G

D

E

P

F

L

F

M

R

R

A

C

K

Q

S

S

E

A

R

R

G

G

I

T

C

S

K

R

V

V

I

I

C

C

F

F

S

S

P

P

R

D

H
|
H

D

S

L

K

T

T

I

L

P

P

E

E

L

L

D

S

V

V

Q

A

A

A

I

L

T

T

K

E

V

I

V

V

E

K

L

T

W

W

K

Q

K

E

E

Q

Y

T

Q

A

E

E

L

L

G

G

G

K

K

T

D

-

F

-

I

Y

N

P

H

W

V

V

Q

Q

I

V

F

F

E

E

N
|
N

K

K

G

G

S

A

V

A

M

M

G

G

C
|
C

S
|
S

N
|
N

P

P

H
|
H

P

P

H
|
H

G

G

Q
|
Q

I

I

W

W

A

A

Q

N

E

S

S

F

I

L

P

P

V

N

E

E

P

A

A

E

K

R

K
x
E

Q

D

V

R

K

L

F

Q

G

K

E

E

Y

Y

Y

F

Q

A

K

E

Y

Q

G

K

R

S

S

P

M

M

V

L

L

V

D

D

Y

Y

V

V

Y

Q

E

R

E

E

L

L

K

A

K

D

G

G

E

S

R

R

I

T

L

V

F

V

E

E

N

T

D

E

Y

H

F

W

V

L

G

A

L

V

V

V

P

P

F
x
Y

W

W

A

A

V

A

W

W

P

P

F

F

E

E

A

T

M

L

I

L

A

L

P

P

K

K

T

A

H

H

I

V

A

L

S

R

L

I

S

T

E

D

M

L

D

T

A

D

A

A

Q

Q

M

R

E

S

A

D

L

L

A

A

D

L

A

A

Y

L

R

K

Q

K

L

L

A

T

I

S

M

R

Y

Y

D

D

N

N

V

L

F

F

K

Q

V

C

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

I

W

H
|
H

Q

G

A

A

P

P

T

F

D

N

G

G

Q

E

N

E

H

N

P

Q

E

H

W

W

D

Q

L

L

H
|
H

M

A

V
x
H

F

F

Y

Y

P

P

L

L

R

R

S

S

A

A

T

T

V

V

K

R

K

K

F

F

M

M

V

V

G

G

Y

Y

E

E

M

M

L

L

A

A

N

E

P

T

Q

Q

R

R

D

D

I

L

T

T

A

A

E

E

S

Q

A

A

V

A

K

E

I

R

L

L

K

R

S

A

Q

V

P

S

K

D

E

I

H

H

Y

F

K

R

active site: C35 (= C52), C38 (= C55), H98 (= H115), N136 (= N155), S144 (= S163), H147 (= H166), H149 (= H168), Q151 (= Q170)
binding beta-mercaptoethanol: F3 (= F4), P5 (≠ F6), H10 (= H11), N136 (= N155), C143 (= C162), Q151 (= Q170), Y208 (≠ F227)
binding fe (iii) ion: E165 (≠ K184), H264 (= H283), H279 (= H298), H281 (≠ V300)
binding uridine-5'-diphosphate: R43 (= R60), V44 (≠ I61), F58 (= F75), N60 (= N77), D61 (= D78), S144 (= S163), N145 (= N164)
binding zinc ion: C35 (= C52), C38 (= C55), H98 (= H115), H147 (= H166)

P07902 Galactose-1-phosphate uridylyltransferase; Gal-1-P uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase; EC 2.7.7.12 from Homo sapiens (Human) (see 22 papers)
46% identity, 99% coverage: 4:347/347 of query aligns to 24:368/379 of P07902

query
sites
P07902

F

F

N

R

F

A

E

N

D

D

H

H

S

Q

H

H

R
x
I

R

R

Y
|
Y

N

N

P

P

F

L

T

Q

G

D

D

E

W

W

L

V

Q

L

V
|
V

S

S

P

A

H

H

R

R

G

M

K

K

R

R

P

P

W

W

Q

Q

G

G

Q

Q

E

V

E

E

D

P

T

Q

A

L

E
x
L

A

K

Q

T

K

V

P

P

A

R

Y

H

D

D

E

P

K

L

C

N

Y

P

L
|
L

C

C

P

P

G

G

N

A

T

I

R

R

I

A

N

N

G

G

E

E

K

V

N

N

P

P

D

Q

Y

Y

T

D

G

S

A

T

Y

F

V

L

F

F

Q

D

N

N

D

D

F

F

G

P

A

A

L

L

T

Q

S

P

D

D

I

A

P

P

Q

S

G

P

E

G

M

P

S

S

E

D

G

H

E

P

F

L

F

F

R

Q

A

A

K

K

S

S

E

A

R

R

G

G

I

V

C

C

K

K

V

V

I

M

C

C

F

F

S
x
H

P

P

R

W

H
x
S

D

D

L

V

T
|
T

I

L

P

P

E

L

L
x
M

D

S

V

V

Q

P

A

E

I

I

T
x
R

K

A

V

V

V
|
V

E

D

L

A

W

W

K

A

K

S

E

V

Y

T

Q

E

E

E

L

L

G

G

G

A

K

Q

D

-

F

-

I

Y

N

P

H

W

V
|
V

Q

Q

I
|
I

F
|
F

E

E

N

N

K

K

G
|
G

S

A

V

M

M

M

G

G

C

C

S

S

N

N

P

P

H

H

P
|
P

H
|
H

G

C

Q
|
Q

I

V

W

W

A

A

Q

S

E

S

S

F

I
x
L

P

P

V

D

E

I

P

A

A

Q

K
x
R

K
x
E

Q

E

V

R

K

S

F

Q

G

Q

E

A

Y

Y

Y

K

Q

S

K

Q

Y

H

G

G

R

E

S

P

M

L

V

L

L

M

D
x
E

Y

Y

V

S

Y
x
R

E

Q

E

E

L

L

K
x
L

K

R

G

K

E

E

R
|
R

I

L

L

V

F

L

E

T

N

S

D

E

Y

H

F

W

V

L

G

V

L

L

V

V

P

P

F

F

W

W

A

A

V

T

W

W

P

P

F

Y

E

Q

A

T

M

L

I

L

A

L

P

P

K

R

T
x
R

H

H

I

V

A

R

S

R

L

L

S

P

E

E

M

L

D

T

A

P

A

A

Q

E

M

R

E

D

A

D

L

L

A

A

D

S

A
x
I

Y

M

R

K

Q

K

L

L

A

L

I

T

M
x
K

Y

Y

D

D

N

N

V
x
L

F

F

K
x
E

V

T

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

I

W

H
|
H

Q

G

A

A

P

P

T

T

D

G

-

S

-

E

-

A

G

G

Q

A

N

N

H

W

P
x
N

E

H

W

W

D

Q

L

L

H
|
H

M

A

V
x
H

F

Y

Y

Y

P

P

L

L

L
|
L

R

R

S

S

A
|
A

T

T

V

V

K
x
R

K

K

F

F

M

M

V

V

G

G

Y

Y

E

E

M

M

L

L

A

A

N

Q

P

A

Q

Q

R

R

D

D

I

L

T
|
T

A

P

E

E

S

Q

A

A

V

A

K

E

I

R

L

L

K

R

S

A

Q

L

P

P

K

E

E

V

H

H

Y

Y

K

H

V

L

I32 (≠ R12) to N: in GALAC1; mild; dbSNP:rs111033644
Y34 (= Y14) to N: in GALAC1; affects protein stability; dbSNP:rs111033836
V44 (= V24) to M: in GALAC1; reduced enzyme activity; dbSNP:rs111033647
L62 (≠ E42) to M: in dbSNP:rs1800461
L74 (= L54) to P: in GALAC1; reduced enzyme activity; dbSNP:rs111033663
H132 (≠ S112) to Q: in GALAC1; affects protein stability; dbSNP:rs367543256
S135 (≠ H115) to L: in GALAC1; about 5% of normal galactose uridylyltransferase activity; dbSNP:rs111033690
T138 (= T118) to M: in GALAC1; mild; dbSNP:rs111033686
M142 (≠ L122) to K: in GALAC1; 4% of normal activity; dbSNP:rs111033695
R148 (≠ T128) to W: in GALAC1; unstable protein; dbSNP:rs111033693
V151 (= V131) to A: in GALAC1; approximately 3% of normal activity; dbSNP:rs111033701
V168 (= V150) to L: in GALAC1; loss of activity; dbSNP:rs367543258
I170 (= I152) to T: in GALAC1; loss of activity; dbSNP:rs111033839
F171 (= F153) to S: in GALAC1; reduced enzyme activity; dbSNP:rs111033715
G175 (= G157) to D: in GALAC1; strongly reduces galactose uridylyltransferase activity; dbSNP:rs111033718
P185 (= P167) to H: in GALAC1; loss of activity; dbSNP:rs111033722
H186 (= H168) active site, Tele-UMP-histidine intermediate
Q188 (= Q170) to R: in GALAC1; most common mutation; 10% of normal galactose uridylyltransferase activity; impairs protein folding; dbSNP:rs75391579
L195 (≠ I177) to P: in GALAC1; no enzymatic activity; dbSNP:rs111033728
R201 (≠ K183) to C: in GALAC1; 2-fold decrease in activity; dbSNP:rs111033739
E202 (≠ K184) binding
E220 (≠ D202) to K: in GALAC1; 3-fold decrease in activity; dbSNP:rs111033747
R223 (≠ Y205) to S: in GALAC1; 3-fold decrease in activity; dbSNP:rs111033750
L227 (≠ K209) to P: in GALAC1; results in no detectable protein in the soluble fraction; dbSNP:rs111033846
R231 (= R213) to H: in GALAC1; 15% of normal activity; dbSNP:rs111033754
R259 (≠ T241) to Q: in GALAC1; loss of activity; dbSNP:rs886042070; to W: in GALAC1; mild; dbSNP:rs786204763
I278 (≠ A260) to N: in GALAC1; 18-fold decrease in activity; dbSNP:rs111033778
K285 (≠ M267) to N: in GALAC1; severe; impairs protein folding; nearly abolishes enzyme activity; dbSNP:rs111033773
L289 (≠ V271) to F: in GALAC1; 3-fold decrease in activity; dbSNP:rs111033774
E291 (≠ K273) to V: in GALAC1; 2-fold decrease in activity; dbSNP:rs111033841
H301 (= H283) binding
N314 (≠ P293) to D: in GALAC1; allele Duarte; exists as allelic variants Duarte-1 and Duarte-2; Duarte-1 has normal or increased activity; Duarte-2 has activity reduced to about 35-45% of normal; dbSNP:rs2070074
H319 (= H298) binding
H321 (≠ V300) binding
L327 (= L306) to P: in GALAC1; results in no detectable protein in the soluble fraction; dbSNP:rs111033832
A330 (= A309) to V: in GALAC1; mild; dbSNP:rs111033804
R333 (≠ K312) to W: in GALAC1; no enzymatic activity; dbSNP:rs111033800
T350 (= T329) to A: in GALAC1; mild; dbSNP:rs111033817

6gqdA Structure of human galactose-1-phosphate uridylyltransferase (galt), with crystallization epitope mutations a21y:a22t:t23p:r25l
46% identity, 100% coverage: 2:347/347 of query aligns to 2:344/344 of 6gqdA

query
sites
6gqdA

T

T

D

P

F

F

N

L

F

A

E

N

D

D

H

H

S

Q

H

H

R

I

R

R

Y

Y

N

N

P

P

F

L

T

Q

G

D

D

E

W

W

L

V

Q

L

V

V

S

S

P

A

H

H

R

R

G

M

K

K

R

R

P

P

W

W

Q

Q

G

G

Q

Q

E

L

D

K

T

T

A

V

E

-

A

-

Q

-

K

-

P

P

A

R

Y

H

D

D

E

P

K

L

C
x
N

Y
x
P

L

L

C
|
C

P

P

G

G

N

A

T

I

R

R

I
x
A

N

N

G

G

E

E

K

V

N

N

P

P

D

Q

Y

Y

T

D

G

S

A

T

Y

F

V

L

F

F

Q

D

N
|
N

D
|
D

F

F

G

P

A

A

L

L

T

Q

S

P

D

D

I

A

P

P

Q

S

G

P

E

G

M

P

S

S

E

D

G

H

E

P

F

L

F

F

R

Q

A

A

K

K

S

S

E

A

R

R

G

G

I

V

C

C

K

K

V

V

I

M

C

C

F

F

S

H

P

P

R

W

H
x
S

D

D

L

V

T

T

I

L

P

P

E

L

L

M

D

S

V

V

Q

P

A

E

I

I

T

R

K

A

V

V

E

D

L

A

W

W

K

A

K

S

E

V

Y

T

Q

E

E

E

L

L

G

G

G

A

K

Q

D

-

F

-

I

Y

N

P

H

W

V

V

Q

Q

I

I

F

F

E

E

N
|
N

K

K

G

G

S

A

V

M

M

M

G

G

C

C

S
|
S

N

N

P

P

H
|
H

P

P

H
|
H

G

C

Q
|
Q

I

V

W

W

A

A

Q

S

E

S

S

F

I

L

P

P

V

D

E

I

P

A

A

Q

K

R

K
x
E

Q

E

V

R

K

S

F

Q

G

Q

E

A

Y

Y

Y

K

Q

S

K

Q

Y

H

G

G

R

E

S

P

M

L

V

L

L

M

D

E

Y

Y

V

S

Y

R

E

Q

E

E

L

L

K

L

K

R

G

K

E

E

R

R

I

L

L

V

F

L

E

T

N

S

D

E

Y

H

F

W

V

L

G

V

L

L

V

V

P

P

F

F

W

W

A

A

V

T

W

W

P

P

F

Y

E

Q

A

T

M

L

I

L

A

L

P

P

K

R

T

R

H

H

I

V

A

R

S

R

L

L

S

P

E

E

M

L

D

T

A

P

A

A

Q

E

M

R

E

D

A

D

L

L

A

A

D

S

A

I

Y

M

R

K

Q

K

L

L

A

L

I

T

M

K

Y

Y

D

D

N

N

V

L

F

F

K

E

V

T

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

I

W

H
|
H

Q

G

A

A

P

P

T

T

D

G

-

S

-

E

-

A

G

G

Q

A

N

N

H

W

P

D

E

H

W

W

D

Q

L

L

H
|
H

M

A

V
x
H

F

Y

Y

Y

P

P

L

L

R

R

S

S

A

A

T

T

V

V

K

R

K

K

F

F

M

M

V

V

G

G

Y

Y

E

E

M

M

L

L

A

A

N

Q

P

A

Q

Q

R

R

D

D

I

L

T

T

A

P

E

E

S

Q

A

A

V

A

K

E

I

R

L

L

K

R

S

A

Q

L

P

P

K

E

E

V

H

H

Y

Y

K

H

V

L

active site: N48 (≠ C52), C51 (= C55), S111 (≠ H115), N149 (= N155), S157 (= S163), H160 (= H166), H162 (= H168), Q164 (= Q170)
binding 5,6-dihydrouridine-5'-monophosphate: P49 (≠ Y53), A57 (≠ I61), N73 (= N77), D74 (= D78), H162 (= H168), Q164 (= Q170)
binding zinc ion: E178 (≠ K184), H277 (= H283), H295 (= H298), H297 (≠ V300)

5in3A Crystal structure of glucose-1-phosphate bound nucleotidylated human galactose-1-phosphate uridylyltransferase (see paper)
44% identity, 97% coverage: 8:345/347 of query aligns to 1:324/324 of 5in3A

query
sites
5in3A

D

D

H

H

S

Q

H

H

R

I

R

R

Y

Y

N

N

P

P

F

L

T

Q

G

D

D

E

W

W

L

V

Q

L

V

V

S

S

P

A

H

H

R

R

G

T

K

V

R

-

P

-

W

-

Q

-

G

-

Q

-

E

-

D

-

T

-

A

-

E

-

A

-

Q

-

K

-

P

P

A

R

Y

H

D

D

E

P

K

L

C
x
N

Y
x
P

L

L

C
|
C

P

P

G

G

N

A

T

I

R

R

I
x
A

N

N

G

G

E

E

K

V

N

N

P

P

D

Q

Y

Y

T

D

G

S

A

T

Y

F

V

L

F
|
F

Q

D

N
|
N

D
|
D

F

F

G

P

A

A

L

L

T

Q

S

P

D

D

I

A

P

P

Q

S

G

P

E

G

M

P

S

S

E

D

G

H

E

P

F

L

F

F

R

Q

A

A

K

K

S

S

E

A

R

R

G

G

I

V

C

C

K

K

V
|
V

I

M

C

C

F

F

S

H

P

P

R

W

H
x
S

D

D

L

V

T

T

I

L

P

P

E

L

L

M

D

S

V

V

Q

P

A

E

I

I

T

R

K

A

V

V

E

D

L

A

W

W

K

A

K

S

E

V

Y

T

Q

E

E

E

L

L

G

G

G

A

K

Q

D

-

F

-

I

Y

N

P

H

W

V

V

Q

Q

I

I

F
|
F

E

E

N
|
N

K

K

G

G

S

A

V

M

M

M

G

G

C

C

S
|
S

N

N

P

P

H
|
H

P

P

H
|
H

G

C

Q
|
Q

I

V

W

W

A

A

Q

S

E

S

S

F

I

L

P

P

V

D

E

I

P

A

A

Q

K

R

K
x
E

Q

E

V

R

K

S

F

Q

G

Q

E

A

Y

Y

Y

K

Q

S

K

Q

Y

H

G

G

R

E

S

P

M

L

V

L

L

M

D

E

Y

Y

V

S

Y

R

E

Q

E

E

L

L

K

L

K

R

G

K

E

E

R

R

I

L

L

V

F

L

E

T

N

S

D

E

Y

H

F

W

V

L

G

V

L

L

V

V

P

P

F

F

W

W

A

A

V

T

W

W

P

P

F

Y

E

Q

A

T

M

L

I

L

A

L

P

P

K

R

T

R

H

H

I

V

A

R

S

R

L

L

S

P

E

E

M

L

D

T

A

P

A

A

Q

E

M

R

E

D

A

D

L

L

A

A

D

S

A

I

Y

M

R

K

Q

K

L

L

A

L

I

T

M

K

Y

Y

D

D

N

N

V

L

F

F

K

E

V

T

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

I

W

H
|
H

Q

G

A

A

P

P

T

T

D

G

-

S

-

E

-

A

G

G

Q

A

N

N

H

W

P

N

E

H

W

W

D

Q

L

L

H
|
H

M

A

V
x
H

F

Y

Y

Y

P

P

L

L

R

R

S

S

A

A

T

T

V

V

K

R

K

K

F

F

M

M

V
|
V

G
|
G

Y

Y

E
|
E

M

M

L

L

A

A

N

Q

P

A

Q

Q

R

R

D

D

I

L

T

T

A

P

E

E

S

Q

A

A

V

A

K

E

I

R

L

L

K

R

S

A

Q

L

P

P

K

E

E

V

H

H

Y

Y

active site: N30 (≠ C52), C33 (= C55), S93 (≠ H115), N131 (= N155), S139 (= S163), H142 (= H166), H144 (= H168), Q146 (= Q170)
binding 1-O-phosphono-alpha-D-glucopyranose: F129 (= F153), N131 (= N155), Q146 (= Q170), V295 (= V316), G296 (= G317), E298 (= E319)
binding 5,6-dihydrouridine-5'-monophosphate: P31 (≠ Y53), A39 (≠ I61), F53 (= F75), N55 (= N77), D56 (= D78), V86 (= V108), H144 (= H168), Q146 (= Q170)
binding zinc ion: E160 (≠ K184), H259 (= H283), H277 (= H298), H279 (≠ V300)

6k5zB Structure of uridylyltransferase (see paper)
30% identity, 79% coverage: 13:286/347 of query aligns to 4:255/314 of 6k5zB

query
sites
6k5zB

R

R

Y

K

N

D

P

P

F

F

T

T

G

G

D

E

W

Y

L

I

Q

L

V

V

S

S

P

P

H

H

R

R

G

L

K

K

R

R

P

P

W

W

Q

Q

G

P

Q

-

E

-

D

-

T

-

A

-

E

-

A

-

Q

-

K

-

P

-

A

-

Y

-

D

E

E

G

K

A

C
|
C

Y

P

L

F

C
|
C

P

P

G

G

N

A

T

P

R

E

I

T

N

G

G

R

E

G

K

W

N

D

P

-

D

-

Y

-

T

-

G

-

A

V

Y

L

V

I

F

L

Q

P

N

N

D

R

F

Y

G

P

A

V

L

V

T

T

S

E

D

N

I

P

P

P

Q

-

G

-

E

E

M

P

S

T

E

A

G

E

E

D

F

L

F

Y

R

E

A

V

K

I

S

P

E

A

R

R

G

G

I

S

C

S

K

L

V

V

I

V

C

V

F

E

S

T

P

P

R

Q

H
|
H

D

D

L

V

T

D

-

D

-

L

-

S

-

D

I

L

P

P

E

L

L

G

D

Q

V

I

Q

K

A

K

I

I

-

L

T

T

K

A

V

V

V

A

E

E

L

A

W

Q

K

R

K

K

E

A

Y

E

Q

K

E

E

L

G

G

N

G

A

K

A

D

Y

F

F

I

L

N

-

H

-

V

-

Q

-

I

F

F

F

E

R

N
|
N

K

K

G

G

S

K

V

E

M

I

G

G

C
x
V

S
|
S

N
x
L

P

T

H
|
H

P

P

H
|
H

G

S

Q
|
Q

I

I

W

Y

A

I

Q

-

E

-

S

-

I

L

P

P

V

V

E

V

P

P

A

P

K

R

K

V

Q

R

V

A

K
x
E

F

L

G

Q

-

A

-

S

-

Y

E

E

Y

W

Y

Y

Q

V

K

K

Y

H

G

G

R

S

S
x
C

M

L

V

H

L
x
C

D

R

Y

I

V

V

Y

E

E

K

E

E

L

E

K

K

K

-

G

-

E

-

R

R

I

L

L

V

F

F

E

Q

N

N

D

R

Y

N

F

W

V

K

G

A

L

F

V

V

P

P

F

F

W

Y

A

A

V

K

W

W

P

P

F

H

E

E

A

V

M

H

I

I

A

Y

P

P

K

K

T

R

H
|
H

I

R

A

S

S

L

L

L

S

T

E

E

M

L

D

T

A

D

A

E

Q

E

M

V

E

A

A

D

L

L

A

A

D

E

A

A

Y

L

R

K

Q

I

L

T

A

L

I

C

M

A

Y

L

D

K

N

Q

V

V

F

A

K

G

V

I

S

P

F

M

P

P

Y

Y

S

I

A

M

G

V

I

L

H
|
H

Q

Q

A

A

P

P

active site: C30 (= C52), C33 (= C55), H86 (= H115), N127 (= N155), S135 (= S163), H138 (= H166), H140 (= H168), Q142 (= Q170)
binding fe (iii) ion: E156 (≠ K187), H252 (= H283)
binding phosphate ion: V134 (≠ C162), S135 (= S163), L136 (≠ N164), H140 (= H168)
binding zinc ion: C30 (= C52), C33 (= C55), H86 (= H115), H138 (= H166), C170 (≠ S198), C173 (≠ L201), H211 (= H242)

Sites not aligning to the query:

Q9FK51 ADP-glucose phosphorylase; ADP-glucose:phosphate adenylyltransferase; EC 2.7.7.- from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
26% identity, 94% coverage: 13:338/347 of query aligns to 26:346/351 of Q9FK51

query
sites
Q9FK51

R

R

Y

K

N

D

P

P

F

V

T

T

G

N

D

R

W

W

L

V

Q

I

V

F

S

S

P

P

H

A

R
|
R

G
x
A

K
|
K

R
|
R

P

P

W

T

Q

D

G

F

Q

K

E

S

E

K

D

S

T

P

A

Q

E

N

A

P

Q

N

-

P

K

K

P

P

A

S

Y

-

D

-

E

-

K

S

C
|
C

Y

P

L

F

C
|
C

P

I

G

G

N

R

T

E

R

Q

I
x
E

N
x
C

G
x
A

E

P

-

E

-

L

-

F

K

R

N

V

P

P

D

D

Y

H

T

D

G

P

A

N

Y

W

-

K

-

L

-

R

V

V

F

I

Q

E

N
|
N

D

L

F

Y

G

P

A

A

L

L

T

S

S

R

D

N

I

L

-

E

P

T

Q

Q

G

S

E

T

M

Q

S

P

E

E

G

T

E

G

F

T

F

S

R

R

A

T

K

I

S

V

E

G

R

F

G

G

I

F

C

H

K

D

V

V

I

V

C

I

F

E

S

S

P

P

R

V

H
|
H

D

S

L

I

T

Q

I

L

P

S

E

D

L

I

D

D

V

P

Q

V

A

G

I

I

T

G

K

D

V

I

V

L

E

I

L

A

W

Y

K

K

E

R

Y

I

Q

N

E

Q

L

I

G

A

G

Q

K

H

D

D

F

S

I

I

N

N

H

Y

V

I

Q

Q

I

V

F

F

E

K

N
|
N

K

Q

G

G

S

A

V

S

M

A

G
|
G

C
x
A

S
|
S

N
x
M

P

S

H
|
H

P

S

H
|
H

G

S

Q
|
Q

I

M

W

M

A

A

Q

L

E

P

S

V

I

V

P

P

V

P

E

T

P

V

A

S

K

S

K

R

Q

L

V

D

K

G

F

T

G

K

E

D

Y

Y

Y

F

Q

E

K

E

Y

T

G

G

R

K

S
x
C

M

C

V

L

L
x
C

D

-

Y

-

V

-

Y

-

E

-

E

E

L

A

K

K

K

S

G

K

E

H

R

F

I

V

L

I

F

D

E

E

N

S

D

S

Y

H

F

F

V

V

G

S

L

V

V

A

P

P

F

F

W

A

A

A

V

T

W

Y

P

P

F

F

E

E

A

I

M

W

I

I

A

I

P

P

K

K

T

D

H
|
H

I

S

A

S

S

H

L

F

S

H

E

H

M

L

D

D

A

D

A

V

Q

K

M

A

E

V

A

D

L

L

A

G

D

G

A

L

Y

-

R

-

Q

-

L

L

A

K

I

L

M

M

Y

L

D

Q

N

K

V

I

F

A

K

K

V

Q

-

L

-

N

S

D

F

P

P

P

Y

Y

S

N

A

Y

G

M

I

I

H

H

Q

T

A

S

P

P

-

L

-

K

-

V

T

T

D

E

G

S

Q

Q

-

L

N

P

H

Y

P

T

E
x
H

W

W

D

F

L

L

H

Q

M

I

V

V

F

-

Y

-

P

-

P

P

L

Q

L

L

R

-

S

-

A

S

T
x
G

V

V

K

G

F
|
F

M
x
E

V

I

G

G

Y

T

E

G

M

C

L

Y

A

I

N

N

P

P

Q

-

R

-

D

-

I

V

T

F

A

P

E

E

S

D

A

V

V

A

K

K

I

V

L

M

K

R

RAKR 41:44 (≠ RGKR 28:31) binding
C63 (= C52) binding
C66 (= C55) binding
ECA 72:74 (≠ ING 61:63) binding
N94 (= N77) binding
H133 (= H115) binding
N173 (= N155) binding
GASM 179:182 (≠ GCSN 161:164) binding
H184 (= H166) binding
H186 (= H168) active site, Tele-AMP-histidine intermediate
Q188 (= Q170) binding
C216 (≠ S198) binding
C219 (≠ L201) binding
H255 (= H242) binding
H310 (≠ E294) binding
G321 (≠ T310) binding
FE 325:326 (≠ FM 314:315) binding

6k9zA Structure of uridylyltransferase mutant (see paper)
28% identity, 79% coverage: 13:286/347 of query aligns to 4:248/309 of 6k9zA

query
sites
6k9zA

R

R

Y

K

N

D

P

P

F

F

T

T

G

G

D

E

W

Y

L

I

Q

L

V

V

S

S

P

P

H

-

R

-

G

-

K

-

R

-

P

-

W

-

Q

-

G

-

Q

Q

E

P

E

E

D

G

T

A

A

-

E

-

A

-

Q

-

K

-

P

-

A

-

Y

-

D

-

E

-

K

-

C
|
C

Y
x
P

L

F

C
|
C

P

P

G

G

N

A

T

P

R

E

I

T

N

G

G

R

E

G

K

W

N

D

P

-

D

-

Y

-

T

-

G

-

A

V

Y

L

V

I

F

L

Q

P

N
|
N

D
x
R

F
x
Y

G

P

A

V

L

V

T

T

S

E

D

N

I

P

P

P

Q

-

G

-

E

E

M

P

S

T

E

A

G

E

E

D

F

L

F

Y

R

E

A

V

K

I

S

P

E

A

R

R

G

G

I

S

C

S

K

L

V

V

I

V

C

V

F

E

S

T

P

P

R

Q

H
|
H

D

D

L

V

T

D

-

D

-

L

-

S

-

D

I

L

P

P

E

L

L

G

D

Q

V

I

Q

K

A

K

I

I

-

L

T

T

K

A

V

V

V

A

E

E

L

A

W

Q

K

R

K

K

E

A

Y

E

Q

K

E

E

L

G

G

N

G

A

K

A

D

Y

F

F

I

L

N

-

H

-

V

-

Q

-

I

F

F

F

E

R

N
|
N

K

K

G

G

S

K

V

E

M

I

G

G

C

V

S
|
S

N
x
L

P

T

H
|
H

P

P

H
x
F

G

S

Q
|
Q

I

I

W
x
Y

A

I

Q

-

E

-

S

-

I

L

P

P

V

V

E

V

P

P

A

P

K

R

K

V

Q

R

V

A

K
x
E

F

L

G

Q

-

A

-

S

-

Y

E

E

Y

W

Y

Y

Q

V

K

K

Y

H

G

G

R

S

S
x
C

M

L

V

H

L
x
C

D

R

Y

I

V

V

Y

E

E

K

E

E

L

E

K

K

K

-

G

-

E

-

R

R

I

L

L

V

F

F

E

Q

N

N

D

R

Y

N

F

W

V

K

G

A

L

F

V

V

P

P

F

F

W

Y

A

A

V

K

W

W

P

P

F

H

E

E

A

V

M

H

I

I

A

Y

P

P

K

K

T

R

H
|
H

I

R

A

S

S

L

L

L

S

T

E

E

M

L

D

T

A

D

A

E

Q

E

M

V

E

A

A

D

L

L

A

A

D

E

A

A

Y

L

R

K

Q

I

L

T

A

L

I

C

M

A

Y

L

D

K

N

Q

V

V

F

A

K

G

V

I

S

P

F

M

P

P

Y

Y

S

I

A

M

G

V

I

L

H
|
H

Q

Q

A

A

P

P

active site: C23 (= C52), C26 (= C55), H79 (= H115), N120 (= N155), S128 (= S163), H131 (= H166), F133 (≠ H168), Q135 (= Q170)
binding fe (iii) ion: E149 (≠ K187), H245 (= H283)
binding uridine-5'-diphosphate: P24 (≠ Y53), N43 (= N77), R44 (≠ D78), Y45 (≠ F79), L129 (≠ N164), Q135 (= Q170), Y137 (≠ W172)
binding zinc ion: C23 (= C52), C26 (= C55), H79 (= H115), H131 (= H166), C163 (≠ S198), C166 (≠ L201), H204 (= H242)

Sites not aligning to the query:

2h39B Crystal structure of an adp-glucose phosphorylase from arabidopsis thaliana with bound adp-glucose
25% identity, 94% coverage: 13:338/347 of query aligns to 6:308/313 of 2h39B

query
sites
2h39B

R

R

Y

K

N

D

P

P

F

V

T

T

G

N

D

R

W

W

L

V

Q

I

V

F

S

S

P

P

H

A

R
|
R

G

A

K

K

R
|
R

P

P

W

T

Q

D

G

-

Q

-

E

-

D

-

T

-

A

-

E

-

A

-

Q

-

K

-

P

-

A

-

Y

F

D

P

E

S

K

S

C
|
C

Y

P

L
x
F

C
|
C

P

I

G

G

N

R

T

E

R

Q

I

E

N
x
C

G

A

E

P

-

E

-

L

-

F

K

R

N

V

P

P

D

D

Y

H

T

D

G

P

A

N

Y

W

-

K

-

L

-

R

V

V

F

I

Q

E

N
|
N

D
x
L

F
x
Y

G

P

A

A

L

L

T

S

S

R

D

N

I

L

P

-

Q

-

G

-

E

-

M

-

S

-

E

E

G

T

E

Q

F

S

F

T

R

R

A

T

K

I

S

V

E

G

R

F

G

G

I

F

C

H

K

D

V

V

I

V

C

I

F

E

S

S

P

P

R

V

H
|
H

D

S

L

I

T

Q

I

L

P

S

E

D

L

I

D

D

V

P

Q

V

A

G

I

I

T

G

K

D

V

I

V

L

E

I

L

A

W

Y

K

K

E

R

Y

I

Q

N

E

Q

L

I

G

A

G

Q

K

H

D

D

F

S

I

I

N

N

H

Y

V

I

Q

Q

I

V

F
|
F

E

K

N
|
N

K

Q

G

G

S

A

V

S

M

A

G
|
G

C
x
A

S
|
S

N
x
M

P

S

H
|
H

P

S

H
x
G

G

S

Q
|
Q

I

M

W

M

A

A

Q

L

E

P

S

V

I

V

P

P

V

P

E

T

P

V

A

S

K

S

K

R

Q

L

V

D

K

G

F

T

G

K

E

D

Y

Y

Y

F

Q

E

K

E

Y

T

G

G

R

K

S
x
C

M

C

V

L

L
x
C

D

-

Y

-

V

-

Y

-

E

-

E

E

L

A

K

K

K

S

G

K

E

H

R

F

I

V

L

I

F

D

E

E

N

S

D

S

Y

H

F

F

V

V

G

S

L

V

V

A

P

P

F

F

W

A

A

A

V

T

W

Y

P

P

F

F

E

E

A

I

M

W

I

I

A

I

P

P

K

K

T

D

H
|
H

I

S

A

S

S

H

L

F

S

H

E

H

M

L

D

D

A

D

A

V

Q

K

M

A

E

V

A

D

L

L

A

G

D

G

A

L

Y

-

R

-

Q

-

L

L

A

K

I

L

M

M

Y

L

D

Q

N

K

V

I

F

A

K

K

V

Q

-

L

-

N

S

D

F

P

P

P

Y

Y

S

N

A

Y

G

M

I

I

H

H

Q

T

A

S

P

P

-

L

-

K

-

V

T

T

D

E

G

S

Q

Q

-

L

N

P

H

Y

P

T

E
x
H

W

W

D

F

L

L

H

Q

M

I

V

V

F

-

Y

-

P

-

P

P

L

Q

L

L

R

-

S

-

A

S

T

G

V

V

K
x
G

K

G

F
|
F

M
x
E

V

I

G

G

Y

T

E

G

M

C

L

Y

A

I

N

N

P

P

Q

-

R

-

D

-

I

V

T

F

A

P

E

E

S

D

A

V

V

A

K

K

I

V

L

M

K

R

active site: C32 (= C52), C35 (= C55), H95 (= H115), N135 (= N155), S143 (= S163), H146 (= H166), G148 (≠ H168), Q150 (= Q170)
binding adenosine-5'-diphosphate-glucose: R21 (= R28), R24 (= R31), F34 (≠ L54), C42 (≠ N62), N63 (= N77), L64 (≠ D78), Y65 (≠ F79), F133 (= F153), N135 (= N155), G141 (= G161), A142 (≠ C162), S143 (= S163), M144 (≠ N164), Q150 (= Q170), G285 (≠ K312), F287 (= F314), E288 (≠ M315)
binding zinc ion: C32 (= C52), H95 (= H115), H146 (= H166), C178 (≠ S198), C181 (≠ L201), H217 (= H242), H272 (≠ E294)

1z84A X-ray structure of galt-like protein from arabidopsis thaliana at5g18200 (see paper)
24% identity, 94% coverage: 13:338/347 of query aligns to 5:306/311 of 1z84A

query
sites
1z84A

R

R

Y

K

N

D

P

P

F

V

T

T

G

N

D

R

W

W

L

V

Q

I

V

F

S

S

P

P

H

-

R

-

G

-

K

-

R

R

P

P

W

T

Q

D

G

F

Q

K

E

S

E

K

D

S

T

P

A

S

E

S

A

-

Q

-

K

-

P

-

A

-

Y

-

D

-

E

-

K

-

C
|
C

Y

P

L
x
F

C
|
C

P

I

G

G

N

R

T

E

R

Q

I

E

N

C

G

A

E

P

-

E

-

L

-

F

K

R

N

V

P

P

D

D

Y

H

T

D

G

P

A

N

Y

W

-

K

-

L

-

R

V

V

F

I

Q

E

N
|
N

D
x
L

F
x
Y

G

P

A

A

L

L

T

S

S

R

D

N

I

L

P

E

Q

T

G

Q

E

S

M

R

S

T

E

I

G

V

E

G

F

F

F

-

R

-

A

-

K

-

S

-

E

-

R

-

G

G

I

F

C

H

K

D

V

V

I

V

C

I

F

E

S

S

P

P

R

V

H
|
H

D

S

L

I

T

Q

I

L

P

S

E

D

L

I

D

D

V

P

Q

V

A

G

I

I

T

G

K

D

V

I

V

L

E

I

L

A

W

Y

K

K

E

R

Y

I

Q

N

E

Q

L

I

G

A

G

Q

K

H

D

D

F

S

I

I

N

N

H

Y

V

I

Q

Q

I

V

F

F

E

K

N
|
N

K

Q

G

G

S

A

V

S

M

A

G

G

C
x
A

S
|
S

N
x
M

P

S

H
|
H

P

S

H
|
H

G

S

Q
|
Q

I

M

W

M

A

A

Q

L

E

P

S

V

I

V

P

P

V

P

E

T

P

V

A

S

K

S

K

R

Q

L

V

D

K

G

F

T

G

K

E

D

Y

Y

Y

F

Q

E

K

E

Y

T

G

G

R

K

S
x
C

M

C

V

L

L
x
C

D

-

Y

-

V

-

Y

-

E

-

E

E

L

A

K

K

K

S

G

K

E

H

R

F

I

V

L

I

F

D

E

E

N

S

D

S

Y

H

F

F

V

V

G

S

L

V

V

A

P

P

F

F

W

A

A

A

V

T

W

Y

P

P

F

F

E

E

A

I

M

W

I

I

A

I

P

P

K

K

T

D

H
|
H

I

S

A

S

S

H

L

F

S

H

E

H

M

L

D

D

A

D

A

V

Q

K

M

A

E

V

A

D

L

L

A

G

D

G

A

L

Y

-

R

-

Q

-

L

L

A

K

I

L

M

M

Y

L

D

Q

N

K

V

I

F

A

K

K

V

Q

-

L

-

N

S

D

F

P

P

P

Y

Y

S

N

A

Y

G

M

I

I

H

H

Q

T

A

S

P

P

-

L

-

K

-

V

T

T

D

E

G

S

Q

Q

-

L

N

P

H

Y

P

T

E
x
H

W

W

D

F

L

L

H

Q

M

I

V

V

F

-

Y

-

P

-

P

P

L

Q

L

L

R

-

S

-

A

S

T

G

V

V

K

G

F

F

M

E

V

I

G

G

Y

T

E

G

M

C

L

Y

A

I

N

N

P

P

Q

-

R

-

D

-

I

V

T

F

A

P

E

E

S

D

A

V

V

A

K

K

I

V

L

M

K

R

active site: C31 (= C52), C34 (= C55), H93 (= H115), N133 (= N155), S141 (= S163), H144 (= H166), H146 (= H168), Q148 (= Q170)
binding adenosine monophosphate: F33 (≠ L54), N62 (= N77), L63 (≠ D78), Y64 (≠ F79), N133 (= N155), A140 (≠ C162), S141 (= S163), M142 (≠ N164), H146 (= H168), Q148 (= Q170)
binding zinc ion: C31 (= C52), C34 (= C55), H93 (= H115), H144 (= H166), C176 (≠ S198), C179 (≠ L201), H215 (= H242), H270 (≠ E294)

Query Sequence

>Echvi_0695 FitnessBrowser__Cola:Echvi_0695
MTDFNFEDHSHRRYNPFTGDWLQVSPHRGKRPWQGQEEDTAEAQKPAYDEKCYLCPGNTR
INGEKNPDYTGAYVFQNDFGALTSDIPQGEMSEGEFFRAKSERGICKVICFSPRHDLTIP
ELDVQAITKVVELWKKEYQELGGKDFINHVQIFENKGSVMGCSNPHPHGQIWAQESIPVE
PAKKQVKFGEYYQKYGRSMVLDYVYEELKKGERILFENDYFVGLVPFWAVWPFEAMIAPK
THIASLSEMDAAQMEALADAYRQLAIMYDNVFKVSFPYSAGIHQAPTDGQNHPEWDLHMV
FYPPLLRSATVKKFMVGYEMLANPQRDITAESAVKILKSQPKEHYKV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory