SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_0722 Echvi_0722 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6kgyB Hocl-induced flavoprotein disulfide reductase rcla from escherichia coli (see paper)
35% identity, 100% coverage: 1:458/458 of query aligns to 1:441/441 of 6kgyB

query
sites
6kgyB

M

M

E

N

N

K

Y

Y

D

Q

A

A

I

V

V

I

L
x
I

G

G

A

F

G
|
G

Q

K

S

A

G

G

M

K

P

T

L

L

A

A

K

V

K

T

I

L

S

A

K

K

L

A

G

G

L

W

S

R

V

V

A

A

L

L

I

I

E
|
E

K
x
Q

R

S

-

N

-

A

V
x
M

I

Y

G

G

G
|
G

T
|
T

C
|
C

I

I

N

N

D

I

G
|
G

C
|
C

S

I

P

P

T
|
T

K

K

T

T

M

L

V

V

S

H

S

D

A

A

R

Q

V

-

A

-

H

-

I

-

V

-

S

-

R

Q

A

H

A

T

D

D

F

F

G

V

T

R

I

A

L

I

P

-

H

-

Y

-

R

-

I

-

D

-

Q

-

R

-

I

-

V

-

K

-

K

Q

R

R

K

K

D

N

H

E

I

V

V

V

E

N

L

F

F

L

R

R

G

N

G

K

A

N

E

F

K

H

S

N

L

L

R

A

K

D

N

M

E

P

S

N

I

I

D

D

I

V

L

I

M

D

G

G

S

Q

A
|
A

A

E

F

F

K

I

D

N

S

N

R

H

T

S

I

L

Q

R

I

V

T

H

S

R

D

P

T

E

G

G

E

N

I

L

S

E

F

-

I

I

S

H

G

G

T

E

K

K

I

I

F

F

I

I

N
|
N

T
|
T

G
|
G

S

A

E

Q

P

T

R

V

I

V

P

P

E

P

I

I

E

P

G

G

L

I

A

T

D

T

T

T

P

P

-

G

Y

V

L

Y

T

D

S

S

T

T

T

G

I

L

M

L

E

N

L

L

E

K

E

E

T

L

P

P

E

G

H

H

L

L

L

G

I

I

M

L

G

G

Y
|
Y

I

I

G

G

L

V

E
|
E

F

F

A

A

Q

S

M

M

F

F

S

A

R

N

F

F

G

G

S

S

K

K

V

V

T

T

I

I

I

L

D

E

R

A

A

A

E

S

R

L

L

F

V

L

H

P

K

R

E

E

D

D

E

R

D

D

V

I

C

A

K

D

E

N

I

I

S

A

Q

T

I

I

F

L

S

R

E

D

E

Q

G

G

I

V

D

D

T

I

L

I

F

L

N

N

A

A

E

H

V

V

R

E

K

R

V

I

S

S

Y

H

Q

H

D

E

G

N

-

Q

F

V

K

Q

L

V

T

H

T

S

E

E

T

H

P

A

K

Q

G

L

L

A

L

V

D

D

I

A

K

-

G

-

S

-

H

-

L

L

V

I

A

A

T

S

G

G

R

R

I

Q

P

P

S

A

S

T

A

A

H

S

L

L

G

H

L

P

E

E

N

N

T

A

R

G

V

I

K

A

L

V

T

N

D

E

K

R

G

G

F

A

I

T

Q

V

T

V

D

D

D

K

F

R

L

L

Q

H

T

T

S

T

E

A

K

D

H

N

I

I

Y

W

A

A

L

M

G
|
G

D
|
D

V

V

A

T

G

G

S

G

A

L

P

Q

F
|
F

T
|
T

H
x
Y

I

I

A
x
S

Y

L

H

D

D

D

A

Y

H

R

I

I

A

V

F

R

Q

D

H

E

A

L

F

L

K

G

E

E

N

G

A

K

V

R

S

S

K

T

H

D

E

D

R

R

L

K

-

N

V

V

P

P

Y

Y

C

S

V

V

F
|
F

I

M

D

T

P

P

Q

P

L

L

G

S

R

R

I

V

G

G

L

M

N

T

E

E

Q

E

E

Q

A

A

Q

R

A

E

K

S

G

G

I

A

P

D

Y

I

K

Q

T

V

G

V

K

T

F

L

L

P

M

V

K

A

H

A

A

I

G

P

R

R

T

A

L

R

E

V

V

M

D

N

E

D

T

T

R

R

G

G

F

V

F

L

K

K

V

A

Q

I

V

V

D

D

P

N

E

K

S

T

K

Q

K

R

I

M

L

L

G

G

A

A

T

S

I

L

L

L

S

C

L

V

D

D

G

S

G

H

E

E

I

M

L

I

A

N

T

I

L

V

Q

K

M

M

A

V

M

M

V

D

G

A

G

G

V

L

T

P

Y

Y

D

S

T

I

I

L

A

R

T

D

L

Q

P

I

I

F

A

T

H
|
H

P

P

T

S

L

M

A

S

E
|
E

S

S

L

L

N

N

N

D

L

L

M

F

G

S

Q

L

I

V

E

K

active site: C43 (= C41), C48 (= C46), T51 (= T49), Y168 (= Y183), E172 (= E187), H426 (= H443), E431 (= E448)
binding flavin-adenine dinucleotide: I9 (≠ L9), G12 (= G12), E33 (= E33), Q34 (≠ K34), M38 (≠ V36), G41 (= G39), T42 (= T40), G47 (= G45), C48 (= C46), A99 (= A114), N126 (= N142), T127 (= T143), G128 (= G144), G291 (= G309), D292 (= D310), F299 (= F317), T300 (= T318), Y301 (≠ H319), S303 (≠ A321), F333 (= F350)

6kyyA Cu(ii) complex of hocl-induced flavoprotein disulfide reductase rcla from escherichia coli (see paper)
34% identity, 99% coverage: 6:458/458 of query aligns to 2:432/432 of 6kyyA

query
sites
6kyyA

A

A

I

V

V

I

L

I

G

G

A
x
F

G
|
G

Q

K

S

A

G

G

M

K

P

T

L

L

A

A

K

V

K

T

I

L

S

A

K

K

L

A

G

G

L

W

S

R

V

V

A

A

L

L

I

I

E
|
E

K
x
Q

R

S

-

N

-

A

V
x
M

I

Y

G

G

T
|
T

C
|
C

I

I

N

N

D

I

G

G

C
|
C

S

I

P

P

T
|
T

K
|
K

T

T

M

L

V

V

S

H

S

D

A

A

R

Q

V

-

A

-

H

-

I

-

V

-

S

-

R

Q

A

H

A

T

D

D

F

F

G

V

T

R

I

A

L

I

P

-

H

-

Y

-

R

-

I

-

D

-

Q

-

R

-

I

-

V

-

K

-

K

Q

R

R

K

K

D

N

H

E

I

V

V

V

E

N

L

F

F

L

R

R

G

N

G

K

A

N

E

F

K

H

S

N

L

L

R

A

K

D

N

M

E

P

S

N

I

I

D

D

I

V

L

I

M

D

G

G

S

Q

A
|
A

A

E

F

F

K

I

D

N

S

N

R

H

T

S

I

L

Q

R

I

V

T

H

S

L

D

E

T

-

G

-

E

-

I

-

S

-

F

-

I

I

S

H

G

G

T

E

K

K

I

I

F

F

I

I

N
|
N

T
|
T

G
|
G

S

A

E

Q

P

T

R

V

I

V

P

P

E

P

I

I

E

P

G

G

L

I

A

T

D

T

T

T

P

P

-

G

Y

V

L

Y

T

D

S

S

T

T

T

G

I

L

M

L

E

N

L

L

E

K

E

E

T

L

P

P

E

G

H

H

L

L

L

G

I

I

M

L

G

G

Y
|
Y

I
|
I

G

G

L

V

E
|
E

F

F

A

A

Q

S

M

M

F

F

S

A

R

N

F

F

G

G

S

S

K

K

V

V

T

T

I

I

I

L

D

E

R

A

A

A

E

S

R

L

L

F

V

L

H

P

K

R

E

E

D

D

E

R

D

D

V

I

C

A

K

D

E

N

I

I

S

A

Q

T

I

I

F

L

S

R

E

D

E

Q

G

G

I

V

D

D

T

I

L

I

F

L

N

N

A

A

E

H

V

V

R

E

K

R

V

I

S

S

Y

H

Q

H

D

E

G

N

-

Q

F

V

K

Q

L

V

T
x
H

T

S

E

E

T
x
H

P

A

K

Q

G

L

L

A

L

V

D

D

I

A

K

-

G

-

S

-

H

-

L

L

V

I

A

A

T

S

G

G

R
|
R

I

Q

P

P

S

A

S

T

A

A

H

S

L

L

G

H

L

P

E

E

N

N

T

A

R

G

V

I

K

A

L

V

T

N

D

E

K

R

G

G

F

A

I

T

Q

V

T

V

D

D

D

K

F

R

L

L

Q

H

T

T

S

T

E

A

K

D

H

N

I

I

Y

W

A

A

L

M

G

G

D
|
D

V

V

A

T

G

G

S

G

A

L

P

Q

F
|
F

T
|
T

H

Y

I

I

A
x
S

Y

L

H

D

D

D

A

Y

H

R

I

I

A

V

F

R

Q

D

H

E

A

L

F

L

K

G

E

E

N

G

A

K

V

R

S

S

K

T

H

D

E

D

R

R

L

K

-

N

V

V

P

P

Y

Y

C

S

V

V

F

F

I

M

D

T

P

P

Q

P

L

L

G

S

R

R

I

V

G

G

L

M

N

T

E

E

Q

E

E

Q

A

A

Q

R

A

E

K

S

G

G

I

A

P

D

Y

I

K

Q

T

V

G

V

K

T

F

L

L

P

M

V

K

A

H

A

A

I

G

P

R

R

T

A

L

R

E

V

V

M

D

N

E

D

T

T

R

R

G

G

F

V

F

L

K

K

V

A

Q

I

V

V

D

D

P

N

E

K

S

T

K

Q

K

R

I

M

L

L

G

G

A

A

T

S

I

L

L

L

S

C

L

V

D

D

G

S

G

H

E

E

I

M

L

I

A

N

T

I

L

V

Q

K

M

M

A

V

M

M

V

D

G

A

G

G

V

L

T

P

Y

Y

D

S

T

I

I

L

A

R

T

D

L

Q

P

I

I

F

A

T

H
|
H

P

P

T

S

L

M

A

S

E
|
E

S

S

L

L

N

N

N

D

L

L

M

F

G

S

Q

L

I

V

E

K

active site: C39 (= C41), C44 (= C46), T47 (= T49), Y159 (= Y183), E163 (= E187), H417 (= H443), E422 (= E448)
binding copper (ii) ion: C39 (= C41), C44 (= C46), H226 (≠ T249), H229 (≠ T252), T291 (= T318)
binding flavin-adenine dinucleotide: F7 (≠ A11), G8 (= G12), E29 (= E33), Q30 (≠ K34), M34 (≠ V36), T38 (= T40), C39 (= C41), C44 (= C46), K48 (= K50), A95 (= A114), N117 (= N142), T118 (= T143), G119 (= G144), I160 (= I184), R243 (= R270), D283 (= D310), F290 (= F317), T291 (= T318), S294 (≠ A321)

8ajjA Crystal structure of the disulfide reductase mera from staphylococcus aureus (see paper)
35% identity, 99% coverage: 4:455/458 of query aligns to 2:437/442 of 8ajjA

query
sites
8ajjA

Y

Y

D

D

A

L

I

I

V

V

L

I

G

G

A

F

G
|
G

Q

K

S

A

G

G

M

K

P

T

L

L

A

A

K

K

K

Y

I

A

S

A

K

S

L

T

G

G

L

Q

S

H

V

V

A

A

L

V

I

I

E
|
E

K
x
Q

-

S

-

P

R

K

V
x
M

I

Y

G

G

G
|
G

T
|
T

C
|
C

I

I

N

N

D

I

G

G

C
|
C

S

I

P

P

T

S

K
|
K

T

T

M

L

V

V

S

H

S

D

A

G

R

L

V

E

A

G

H

K

I

-

V

-

S

S

R

F

A

E

A

A

D

S

F

Y

G

-

T

-

I

-

L

-

P

-

H

-

Y

-

R

-

I

-

D

-

Q

-

R

-

I

-

V

-

K

-

K

N

R

R

K

K

D

N

H

D

I

V

V

V

E

N

L

A

F

L

R

N

G

N

G

K

A

N

E

Y

K

H

S

L

L

L

R

A

K

D

N

D

E

N

S

N

I

I

D

D

I

V

L

L

M

D

G

F

S

K

A
|
A

A

Q

F

F

K

K

D

S

S

N

R

T

T

E

I

V

Q

N

I

L

T

L

S

D

D

Q

T

H

G

D

E

D

I

I

-

V

S

D

F

S

I

I

S

T

G

A

T

P

K

H

I

I

F

I

I

I

N
|
N

T
|
T

G
|
G

S

A

E

T

P

S

R

V

I

I

P

P

E

N

I

I

E

K

G

G

L

L

A

D

D

Q

T

A

P

K

Y

H

L

V

-

F

T

D

S

S

T

T

T

G

I

L

M

L

E

N

L

I

E

S

E

Y

T

Q

P

P

E

K

H

H

L

L

L

V

I

I

M

V

G

G

Y

Y

I
|
I

G

A

L

L

E

E

F

F

A

A

Q

S

M

M

F

F

S

A

R

N

F

L

G

G

S

S

K

K

V

V

T

T

I

V

I

L

D

E

R

R

A

G

E

E

R

S

L

F

V

M

H

P

K

R

E

E

D

D

E

Q

D

D

V

V

C

V

K

A

E

Y

I

G

S

I

Q

T

I

D

F

L

S

E

E

N

E

K

G

G

I

I

D

A

T

L

L

H

F

T

N

N

A

V

E

E

V

T

R

T

K

E

V

L

S

S

Y

-

Q

S

D

D

G

N

F

H

K

H

L

T

T

T

T

V

E

H

T

T

P

-

K

-

G

N

L

V

L

D

D

N

I

F

K

E

G

A

S

D

H

A

L

V

L

L

V

L

A

A

T

I

G

G

R

R

I

K

P

P

S
x
N

S

T

A

D

H

-

L

L

G

A

L

L

E

E

N

N

T

T

R

D

V

I

K

E

L

L

T

G

D

D

K

R

G

G

F

E

I

I

Q

K

T

V

D

N

D

A

F

H

L

L

Q

Q

T

T

S

T

E

V

K

P

H

H

I

I

Y

Y

A

A

L

A

G
|
G

D
|
D

V

V

A

K

G

G

S

G

A

L

P
x
Q

F
|
F

T
|
T

H
x
Y

I

I

A
x
S

Y

L

H

D

D

D

A

Y

H

R

I

I

A

I

F

K

Q

S

H

A

A

L

F

Y

K

G

E

N

N

Q

A

S

V

R

S

T

K

T

H

D

E

N

R

R

-

G

L

S

V

V

P

P

Y

Y

C

T

V

V

F

F

I

I

D

D

P

P

Q

P

L

L

G

S

R

R

I

V

G

G

L

L

N

T

E

S

Q

K

E

E

A

A

Q

A

A

A

K

Q

G

H

I

Y

P
x
D

Y
|
Y

K

T

T

E

G

H

K

Q

F

L

L

L

M

V

K

S

H

A

A

I

G

P

R

R

T

H

L

K

E

I

V

N

D

N

E

D

T

P

R

R

G

G

F

L

F

F

K

K

V

V

Q

V

V

I

D

N

P

N

E

E

S

N

K

N

K

M

I

I

L

L

G

G

A

A

T

T

I

L

L

Y

S

G

L

K

D

Q

G

S

G

E

E

E

I

L

L

I

A

N

T

I

L

I

Q

K

M

L

A

A

M

I

V

D

G

Q

G

N

V

I

T

P

Y

Y

D

T

T

V

I

L

A

R

T

D

L

N

P

I

I

Y

A

T

H

H

P

P

T

T

L

M

A

A

E

E

S

S

L

F

N

N

N

D

L

L

M

F

G

N

binding flavin-adenine dinucleotide: G10 (= G12), E31 (= E33), Q32 (≠ K34), M36 (≠ V36), G39 (= G39), T40 (= T40), C41 (= C41), C46 (= C46), K50 (= K50), A97 (= A114), N126 (= N142), T127 (= T143), G128 (= G144), I169 (= I184), N255 (≠ S273), G290 (= G309), D291 (= D310), Q297 (≠ P316), F298 (= F317), T299 (= T318), Y300 (≠ H319), S302 (≠ A321)
binding histidine: D353 (≠ P371), Y354 (= Y372)

Sites not aligning to the query:

binding histidine: 441, 442

8ajkB Crystal structure of a c43s variant from the disulfide reductase mera from staphylococcus aureus (see paper)
34% identity, 99% coverage: 1:455/458 of query aligns to 2:440/447 of 8ajkB

query
sites
8ajkB

M

M

E

K

N

T

Y

Y

D

D

A

L

I

I

V

V

L

I

G
|
G

A

F

G
|
G

Q

K

S

A

G

G

M

K

P

T

L

L

A

A

K

K

K

Y

I

A

S

A

K

S

L

T

G

G

L

Q

S

H

V

V

A

A

L

V

I

I

E
|
E

K
x
Q

-

S

-

P

R

K

V
x
M

I

Y

G

G

G
|
G

T
|
T

C

S

I

I

N

N

D

I

G
|
G

C
|
C

S

I

P

P

T

S

K
|
K

T

T

M

L

V

V

S

H

S

D

A

G

R

L

V

E

A

G

H

K

I

-

V

-

S

S

R

F

A

E

A

A

D

S

F

Y

G

-

T

-

I

-

L

-

P

-

H

-

Y

-

R

-

I

-

D

-

Q

-

R

-

I

-

V

-

K

-

K

N

R

R

K

K

D

N

H

D

I

V

V

V

E

N

L

A

F

L

R

N

G

N

G

K

A

N

E

Y

K

H

S

L

L

L

R

A

K

D

N

D

E

N

S

N

I

I

D

D

I

V

L

L

M

D

G

F

S
x
K

A
|
A

A

Q

F

F

K

K

D

S

S

N

R

T

T

E

I

V

Q

N

I

L

T

L

S

D

D

Q

T

H

G

D

E

D

I

I

-

V

S

D

F

S

I

I

S

T

G

A

T

P

K

H

I

I

F

I

I

I

N
|
N

T
|
T

G
|
G

S

A

E

T

P

S

R

V

I

I

P

P

E

N

I

I

E

K

G

G

L

L

A

D

D

Q

T

A

P

K

Y

H

L

V

-

F

T

D

S

S

T

T

T

G

I

L

M

L

E

N

L

I

E

S

E

Y

T

Q

P

P

E

K

H

H

L

L

L

V

I

I

M

V

G

G

Y

Y

I

I

G

A

L

L

E

E

F

F

A

A

Q

S

M

M

F

F

S

A

R

N

F

L

G

G

S

S

K

K

V

V

T

T

I

V

I

L

D

E

R

R

A

G

E

E

R

S

L

F

V

M

H

P

K

R

E

E

D

D

E

Q

D

D

V

V

C

V

K

A

E

Y

I

G

S

I

Q

T

I

D

F

L

S

E

E

N

E

K

G

G

I

I

D

A

T

L

L

H

F

T

N

N

A

V

E

E

V

T

R

T

K

E

V

L

S

S

Y

-

Q

S

D

D

G

N

F

H

K

H

L

T

T

T

T

V

E

H

T

T

P

-

K

-

G

N

L

V

L

D

D

N

I

F

K

E

G

A

S

D

H

A

L

V

L

L

V

L

A

A

T

I

G

G

R

R

I

K

P

P

S

N

S

T

A

-

H

D

L

L

G

A

L

L

E

E

N

N

T

T

R

D

V

I

K

E

L

L

T

G

D

D

K

R

G

G

F

E

I

I

Q

K

T

V

D

N

D

A

F

H

L

L

Q

Q

T

T

S

T

E

V

K

P

H

H

I

I

Y

Y

A

A

L

A

G
|
G

D
|
D

V

V

A

K

G

G

S

G

A

L

P
x
Q

F
|
F

T
|
T

H
x
Y

I

I

A
x
S

Y

L

H

D

D

D

A

Y

H

R

I

I

A

I

F

K

Q

S

H

A

A

L

F

Y

K

G

E

N

N

Q

A

S

V

R

S

T

K

T

H

D

E

N

R

R

-

G

L

S

V

V

P

P

Y

Y

C

T

V

V

F

F

I

I

D

D

P

P

Q

P

L

L

G

S

R

R

I

V

G

G

L

L

N

T

E

S

Q

K

E

E

A

A

Q

A

A

A

K

Q

G

H

I

Y

P

D

Y

Y

K

T

T

E

G

H

K

Q

F

L

L

L

M

V

K

S

H

A

A

I

G

P

R

R

T

H

L

K

E

I

V

N

D

N

E

D

T

P

R

R

G

G

F

L

F

F

K

K

V

V

Q

V

V

I

D

N

P

N

E

E

S

N

K

N

K

M

I

I

L

L

G

G

A

A

T

T

I

L

L

Y

S

G

L

K

D

Q

G

S

G

E

E

E

I

L

L

I

A

N

T

I

L

I

Q

K

M

L

A

A

M

I

V

D

G

Q

G

N

V

I

T

P

Y

Y

D

T

T

V

I

L

A

R

T

D

L

N

P

I

I

Y

A

T

H

H

P

P

T

T

L

M

A

A

E

E

S

S

L

F

N

N

N

D

L

L

M

F

G

N

binding flavin-adenine dinucleotide: G11 (= G10), G13 (= G12), E34 (= E33), Q35 (≠ K34), M39 (≠ V36), G42 (= G39), T43 (= T40), G48 (= G45), C49 (= C46), K53 (= K50), K99 (≠ S113), A100 (= A114), N129 (= N142), T130 (= T143), G131 (= G144), G293 (= G309), D294 (= D310), Q300 (≠ P316), F301 (= F317), T302 (= T318), Y303 (≠ H319), S305 (≠ A321)

5x1yB Structure of mercuric reductase from lysinibacillus sphaericus (see paper)
31% identity, 98% coverage: 2:450/458 of query aligns to 2:446/454 of 5x1yB

query
sites
5x1yB

E

D

N

N

Y

Y

D

D

A

L

I

L

V

I

L
x
I

G

G

A

S

G
|
G

Q
x
A

S

A

G

A

M

F

P

S

L

S

A

A

K

I

K

K

I

A

S

I

K

E

L

Y

G

G

L

A

S

K

V

V

A

G

L

M

I
|
I

E
|
E

K
x
R

R

G

V

T

I
x
V

G

G

G
|
G

T
|
T

C
|
C

I

V

N

N

D

I

G
|
G

C
|
C

S

V

P

P

T
x
S

K
|
K

T

T

M

L

V

L

S

R

S

A

A

G

R

E

V

I

A

I

H

H

I

L

V

S

S

K

R

D

A

N

A

P

D

F

F

I

G

G

T

L

I

Q

L

T

P

S

H
x
A

Y
x
G

R

E

I

V

D

D

Q

L

R

A

I

S

V

L

K

I

K

T

R

Q

K

K

D

D

H

K

I

L

V

V

E

S

L

E

F

L

R

R

G

N

G

Q

A

K

E

Y

K

M

S

D

L

L

R

I

K

D

N

E

E

Y

S

N

I

F

D

D

I

L

L

I

M

K

G

G

S

E

A
|
A

A

K

F

F

K

V

D

D

S

A

R

S

T

T

I

V

Q

E

I

V

T

N

S

G

D

-

T

-

G

-

E

-

I

-

S

A

F

K

I

L

S

S

G

A

T

K

K

R

I

F

F

L

I

I

N

A

T
|
T

G
|
G

S

A

E

S

P

P

R

S

I

L

P

P

E

Q

I

I

E

S

G

G

L

L

A

E

D

K

T

M

P

D

Y

Y

L

L

T

T

S

S

T

T

I

L

M

L

E

E

L

L

E

K

T

I

P

P

E

K

H

R

L

L

L

T

I

V

M

I

G

G

G

S

G

G

Y
|
Y

I

I

G

G

L

M

E
|
E

F

L

A

G

Q

Q

M

L

F

F

S

H

R

H

F

L

G

G

S

S

K

E

V

I

T

T

I

L

I

M

D

Q

R

R

A

S

E

E

R

R

L

L

V

L

H

K

K

E

E

Y

D

D

E

P

D

E

V

I

C

S

K

E

E

S

I

V

S

E

Q

K

I

A

F

L

S

I

E

E

E

Q

G

G

I

I

D

N

T

L

L

V

F

K

N

G

A

A

E

T

V

F

R

E

K

R

V

V

S

E

Y

Q

Q

S

D

G

G

E

F

I

K

K

L

R

T

V

T

Y

E

V

T

T

P

V

K

N

G

G

L

S

L

R

D

E

-

V

I

I

K

E

G

S

S

D

H

Q

L

L

V

V

A

A

T

T

G

G

R
|
R

I

K

P

P

S

N

S

T

A

D

H

S

L

L

G

N

L

L

E

S

N

A

T

A

R

G

V

V

K

E

L

T

T

G

D

K

K

N

G

N

F

E

I

I

Q

L

T

I

D

N

D

D

F

F

L

G

Q

Q

T

T

S

S

E

N

K

E

H

K

I

I

Y

Y

A

A

L

A

G
|
G

D
|
D

V

V

A

T

G

L

S

G

A

P

P

Q

F
|
F

T
x
V

H

Y

I

V

A
|
A

Y
x
A

H

Y

D

E

A

G

H

G

I

I

A

I

F

T

Q

D

H

N

A

A

F

I

K

G

E

G

N

L

A

N

V

K

S

K

K

I

H

D

E

L

R

S

L

V

V

V

P

P

Y

A

C

V

V

T

F

F

I

T

D

N

P

P

Q

T

L

V

G

A

R

T

I

V

G

G

L

L

N

T

E

E

Q

E

E

Q

A

A

Q

K

A

E

K

K

G

G

I

Y

P

D

Y

V

K

K

T

T

G

S

K

V

F

L

L

P

M

L

K

D

H

A

A

V

G

P

R

R

T

A

L

I

E

V

V

N

D

R

E

E

T

T

R

T

G

G

F

V

F

F

K

K

V

L

Q

V

V

A

D

D

P

A

E

E

S

T

K

L

K

K

I

V

L

L

G

G

A

V

T

H

I

I

L

V

S

S

L

E

D

N

G

A

G

G

E

D

I

V

L

I

A

Y

T

A

L

A

Q

S

M

L

A

A

M

V

V

K

G

F

G

G

V

L

T

T

Y

V

D

E

T

D

I

L

A

T

T

E

L

T

P

L

I
x
A

A

P

H
x
Y

P

L

T

T

L

M

A

A

E
|
E

S

G

L

L

active site: A13 (≠ Q13), V37 (≠ I37), C41 (= C41), C46 (= C46), S49 (≠ T49), A74 (≠ H74), G75 (≠ Y75), Y178 (= Y183), E182 (= E187), A318 (≠ Y322), A437 (≠ I441), Y439 (≠ H443), E444 (= E448)
binding flavin-adenine dinucleotide: I9 (≠ L9), G12 (= G12), I32 (= I32), E33 (= E33), R34 (≠ K34), G39 (= G39), T40 (= T40), C41 (= C41), G45 (= G45), C46 (= C46), K50 (= K50), A114 (= A114), T138 (= T143), G139 (= G144), Y178 (= Y183), R266 (= R270), G305 (= G309), D306 (= D310), F313 (= F317), V314 (≠ T318), A317 (= A321)

D9J041 Mercuric reductase; Hg(II) reductase; EC 1.16.1.1 from Lysinibacillus sphaericus (Bacillus sphaericus) (see paper)
31% identity, 98% coverage: 2:450/458 of query aligns to 83:527/546 of D9J041

query
sites
D9J041

E

D

N

N

Y

Y

D

D

A

L

I

L

V

I

L

I

G

G

A

S

G

G

Q

A

S

A

G

A

M

F

P

S

L

S

A

A

K

I

K

K

I

A

S

I

K

E

L

Y

G

G

L

A

S

K

V

V

A

G

L

M

I

I

E

E

K

R

R

G

V

T

I

V

G

G

T

T

C
|
C

I

V

N

N

D

I

G

G

C
|
C

S

V

P

P

T

S

K

K

T

T

M

L

V

L

S

R

S

A

A

G

R

E

V

I

A

I

H

H

I

L

V

S

S

K

R

D

A

N

A

P

D

F

F

I

G

G

T

L

I

Q

L

T

P

S

H

A

Y

G

R

E

I

V

D

D

Q

L

R

A

I

S

V

L

K

I

K

T

R

Q

K

K

D

D

H

K

I

L

V

V

E

S

L

E

F

L

R

R

G

N

G

Q

A

K

E

Y

K

M

S

D

L

L

R

I

K

D

N

E

E

Y

S

N

I

F

D

D

I

L

L

I

M

K

G

G

S

E

A

A

A

K

F

F

K

V

D

D

S

A

R

S

T

T

I

V

Q

E

I

V

T

N

S

G

D

-

T

-

G

-

E

-

I

-

S

A

F

K

I

L

S

S

G

A

T

K

K

R

I

F

F

L

I

I

N

A

T

T

G

G

S

A

E

S

P

P

R

S

I

L

P

P

E

Q

I

I

E

S

G

G

L

L

A

E

D

K

T

M

P

D

Y

Y

L

L

T

T

S

S

T

T

I

L

M

L

E

E

L

L

E

K

T

I

P

P

E

K

H

R

L

L

L

T

I

V

M

I

G

G

G

S

G

G

Y

Y

I

I

G

G

L

M

E

E

F

L

A

G

Q

Q

M

L

F

F

S

H

R

H

F

L

G

G

S

S

K

E

V

I

T

T

I

L

I

M

D

Q

R

R

A

S

E

E

R

R

L

L

V

L

H

K

K

E

E

Y

D

D

E

P

D

E

V

I

C

S

K

E

E

S

I

V

S

E

Q

K

I

A

F

L

S

I

E

E

E

Q

G

G

I

I

D

N

T

L

L

V

F

K

N

G

A

A

E

T

V

F

R

E

K

R

V

V

S

E

Y

Q

Q

S

D

G

G

E

F

I

K

K

L

R

T

V

T

Y

E

V

T

T

P

V

K

N

G

G

L

S

L

R

D

E

-

V

I

I

K

E

G

S

S

D

H

Q

L

L

V

V

A

A

T

T

G

G

R

R

I

K

P

P

S

N

S

T

A

D

H

S

L

L

G

N

L

L

E

S

N

A

T

A

R

G

V

V

K

E

L

T

T

G

D

K

K

N

G

N

F

E

I

I

Q

L

T

I

D

N

D

D

F

F

L

G

Q

Q

T

T

S

S

E

N

K

E

H

K

I

I

Y

Y

A

A

L

A

G

G

D

D

V

V

A

T

G

L

S

G

A

P

P

Q

F

F

T

V

H

Y

I

V

A

A

Y

A

H

Y

D

E

A

G

H

G

I

I

A

I

F

T

Q

D

H

N

A

A

F

I

K

G

E

G

N

L

A

N

V

K

S

K

K

I

H

D

E

L

R

S

L

V

V

V

P

P

Y

A

C

V

V

T

F

F

I

T

D

N

P

P

Q

T

L

V

G

A

R

T

I

V

G

G

L

L

N

T

E

E

Q

E

E

Q

A

A

Q

K

A

E

K

K

G

G

I

Y

P

D

Y

V

K

K

T

T

G

S

K

V

F

L

L

P

M

L

K

D

H

A

A

V

G

P

R

R

T

A

L

I

E

V

V

N

D

R

E

E

T

T

R

T

G

G

F

V

F

F

K

K

V

L

Q

V

V

A

D

D

P

A

E

E

S

T

K

L

K

K

I

V

L

L

G

G

A

V

T

H

I

I

L

V

S

S

L

E

D

N

G

A

G

G

E

D

I

V

L

I

A

Y

T

A

L

A

Q

S

M

L

A

A

M

V

V

K

G

F

G

G

V

L

T

T

Y

V

D

E

T

D

I

L

A

T

T

E

L

T

P

L

I

A

A

P

H

Y

P

L

T

T

L

M

A

A

E

E

S

G

L

L

C122 (= C41) modified: Disulfide link with 127, Redox-active
C127 (= C46) modified: Disulfide link with 122, Redox-active

P16171 Mercuric reductase; Hg(II) reductase; EC 1.16.1.1 from Bacillus cereus (see paper)
30% identity, 98% coverage: 3:450/458 of query aligns to 169:612/631 of P16171

query
sites
P16171

N

D

Y

Y

D

D

A

Y

I

I

V

I

L

I

G

G

A

S

G

G

Q

G

S

A

G

A

M

F

P

S

L

S

A

A

K

I

K

E

I

A

S

V

K

A

L

L

G

N

L

A

S

K

V

V

A

A

L

M

I

I

E

E

K

R

R

G

V

T

I

V

G

G

T

T

C

C

I

V

N

N

D

V

G

G

C

C

S

V

P

P

T

S

K

K

T

T

M

L

V

L

S

R

S

A

A

G

R

E

V

I

A

N

H

H

I

L

V

A

S

K

R

N

A

N

A

P

D

F

F

V

G

G

T

L

I

H

L

T

P

S

H

A

Y

S

R

N

I

V

D

D

Q

L

R

A

I

P

V

L

K

V

K

K

R

Q

K

K

D

N

H

D

I

L

V

V

E

T

L

E

F

M

R

R

G

N

G

E

A

K

E
x
Y

K

V

S

N

L

L

R

I

K

D

N

D

E

Y

S

G

I

F

D

E

I

L

L

I

M

K

G

G

S

E

A

S

A

K

F

F

K

V

D

N

S

E

R

N

T

T

I

V

Q

E

I

V

T

N

S

G

D

N

T

Q

G

-

E

-

I

-

S

-

F

-

I

I

S

T

G

A

T

K

K

R

I

F

F

L

I

I

N

A

T

T

G

G

S

A

E

S

P

S

R

T

I

A

P

P

E

N

I

I

E

P

G

G

L

L

A

D

D

E

T

V

P

D

Y

Y

L

L

T

T

S

S

T

T

T

S

I

L

M

L

E

E

L

L

E

K

T

V

P

P

E

N

H

R

L

L

L

T

I

V

M

I

G

G

G

S

G

G

Y

Y

I

I

G

G

L

M

E

E

F

L

A

G

Q

Q

M

L

F

F

S

H

R

N

F

L

G

G

S

S

K

E

V

V

T

T

I

L

I

I

D

Q

R

R

A

S

E

E

R

R

L

L

V

L

H

K

K

E

E

Y

D

D

E

P

D

E

V

I

C

S

K

E

E

A

I

I

S

T

Q

K

I

A

F

L

S

T

E

E

E

Q

G

G

I

I

D

N

T

L

L

V

F

T

N

G

A

A

E

T

V

Y

R

E

K

R

V

V

S

E

Y

-

Q

Q

D

D

G

G

-

D

-

I

F

K

K

K

L

V

T

H

T

V

E

E

T

I

P

N

K

G

G

K

L

K

L

R

D

I

I

I

K

E

G

A

S

E

H

Q

L

L

V

I

A

A

T

T

G

G

R

R

I

K

P

P

S

I

S

Q

A

T

H

S

L

L

G

N

L

L

E

H

N

A

T

A

R

G

V

V

K

E

L

V

T

G

D

S

K

R

G

G

F

E

I

I

Q

V

T

I

D

D

F

Y

L

L

Q

K

T

T

S

T

E

N

K

S

H

R

I

I

Y

Y

A

S

L

A

G

G

D

D

V

V

A

T

G

P

S

G

A

P

P

Q

F

F

T

V

H

Y

I

V

A

A

Y

A

H

Y

D

E

A

G

H

G

I

L

A

A

F

A

Q

R

H

N

A

A

F

I

K

G

E

G

N

L

A

N

V

Q

S

K

K

V

H

N

E

L

R

E

L

V

V

V

P

P

Y

G

C

V

V

T

F

F

I

T

D

S

P

P

Q

S

L

I

G

A

R

T

I

V

G

G

L

L

N

T

E

E

Q

Q

E

Q

A

A

Q

K

A

E

K

K

G

G

I

Y

P

E

Y

V

K

K

T

T

G

S

K

V

F

L

L

P

M

L

K

D

H

A

A

V

G

P

R

R

T

A

L

L

E

V

V

N

D

R

E

E

T

T

R

T

G

G

F

V

F

F

K

K

V

L

Q

V

V

A

D

D

P

A

E

K

S

T

K

L

K

K

I

V

L

L

G

G

A

A

T

H

I

V

L

V

S

A

L

E

D

N

G

A

G

G

E

D

I

V

L

I

A

Y

T

A

L

A

Q

T

M

L

A

A

M

V

V

K

G

F

G

G

V

L

T

T

Y

V

D

G

T

D

I

L

A

R

T

E

L

T

P

M

I

A

A

P

H
x
Y

P

L

T

T

L

M

A

A

E

E

S

G

L

L

Y264 (≠ E98) mutation to F: 30-fold decrease in activity. 300-fold decrease in activity; when associated with F-605.
Y605 (≠ H443) mutation to F: 10-fold decrease in activity. 300-fold decrease in activity; when associated with F-264.; mutation to H: 2-fold decrease in activity.

4jdrA Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase from escherichia coli (see paper)
30% identity, 97% coverage: 7:450/458 of query aligns to 9:451/471 of 4jdrA

query
sites
4jdrA

I

V

V

V

L

L

G
|
G

A

A

G
|
G

Q
x
P

S
x
A

G

G

M

Y

P

S

L

A

A

A

K

F

K

R

I

C

S

A

K

D

L

L

G

G

L

L

S

E

V

T

A

V

L

I

I

V

E
|
E

K
x
R

-
x
Y

R

N

V

T

I
x
L

G

G

T
x
V

C
|
C

I

L

N

N

D

V

G
|
G

C
|
C

S

I

P

P

T
x
S

K
|
K

T

A

M

L

V

L

S

H

S

V

A

A

R

K

V

V

A

I

H

E

I

E

V

A

S

K

R

A

A

L

A

A

D

E

F

H

G

G

T

I

I

V

L

F

P

G

H
x
E

Y
x
P

R

K

I

T

D

D

Q

I

R

D

I

K

V

I

K

R

K

T

R

W

K

K

D

E

H

K

I

V

V

I

E

N

L

Q

F

L

R

T

G

G

A

L

E

-

K

A

S

G

L

M

R

A

K

K

N

G

E

R

S

K

I

V

D

K

I

V

L

V

M

N

G

G

S
x
L

A
x
G

A

K

F

F

K

T

D

G

S

A

R

N

T

T

I

L

Q

E

I

V

T

E

S

G

D

E

T

N

G

G

E

K

I

-

S

T

F

V

I

I

S

N

G

F

T

D

K

N

I

A

F

I

I

I

N

A

T
x
A

G
|
G

S

S

E

R

P

P

-

I

R

Q

I

L

P

P

E

F

I

I

E

P

G

-

L

H

A

E

D

D

T

P

P

R

Y

I

L

W

T

D

S

S

T

T

T

D

I

A

M

L

E

E

L

L

E

K

E

E

T

V

P

P

E

E

H

R

L

L

I

V

M

M

G

G

Y
x
I

I
|
I

G

G

L

L

E
|
E

F

M

A

G

Q

T

M

V

F

Y

S

H

R

A

F

L

G

G

S

S

K

Q

V

I

T

D

I

V

D

E

R

M

A

F

E

D

R

Q

L

V

V

I

H

P

K

A

E

A

D

D

E

K

D

D

V

I

C

V

K

K

E

V

I

F

S

T

Q

K

I

R

F

I

S

S

E

K

G

-

I

F

D

N

T

L

L

M

F

L

N

E

A

T

E

K

V

V

R

T

K

A

V

V

-

E

S

A

Y

K

Q

E

D

D

G

G

F

I

K

Y

L

V

T

T

T

M

E

E

T

G

P

K

K

K

G

A

L

P

L

A

D

E

I

P

-

Q

K

R

G

Y

S

D

H

A

L

V

L

L

V

V

A

A

T

I

G

G

R

R

I

V

P

P

S

N

S

G

A

K

H

N

L

L

G

D

L

A

E

G

N

K

T

A

R

G

V

V

K

E

L

V

T

D

D

D

K

R

G

G

F

F

I

I

Q

R

T

V

D

D

D

K

F

Q

L

L

Q

R

T

T

S

N

E

V

K

P

H

H

I

I

Y

F

A

A

L

I

G
|
G

D
|
D

V

I

A

V

G

G

S

Q

A

P

P
x
M

F
x
L

T
x
A

H
|
H

I

K

A

G

Y
x
V

H

H

D

E

A

G

H

H

I

V

A

A

F

A

Q

E

H

V

A

I

F

-

K

-

E

-

N

-

A

A

V

G

S

K

K

K

H

H

-

Y

-

F

-

D

E

P

R

K

L

V

V

I

P

P

Y

S

C

I

V

A

F

Y

I

T

D

E

P

P

Q

E

L

V

G

A

R

W

I

V

G

G

L

L

N

T

E

E

Q

K

E

E

A

A

Q

K

A

E

K

K

G

G

I

I

P

S

Y

Y

K

E

T

T

G

A

K

T

F

F

L

P

M

W

K

A

H

A

A

S

G

G

R

R

T

A

L

I

E

A

V

S

D

D

E

C

T

A

R

D

G

G

F

M

F

T

K

K

V

L

Q

I

V

F

D

D

P

K

E

E

S

S

K

H

K

R

I

V

L

I

G

G

A

G

T

A

I

I

L

V

S

G

L

T

D

N

G

G

E

E

I

L

L

L

A

G

T

E

L

I

Q

G

M

L

A

A

M

I

V

E

G

M

G

G

V

C

T

D

Y

A

D

E

T

D

I

I

A

A

T

L

L

T

P

I

I
x
H

A

A

H
|
H

P

P

T

T

L

L

A

H

E
|
E

S

S

L

V

active site: P15 (≠ Q13), L40 (≠ I37), C44 (= C41), C49 (= C46), S52 (≠ T49), E77 (≠ H74), P78 (≠ Y75), I184 (≠ Y183), E188 (= E187), V324 (≠ Y322), H442 (≠ I441), H444 (= H443), E449 (= E448)
binding flavin-adenine dinucleotide: G12 (= G10), G14 (= G12), P15 (≠ Q13), A16 (≠ S14), E35 (= E33), R36 (≠ K34), Y37 (vs. gap), V43 (≠ T40), C44 (= C41), G48 (= G45), C49 (= C46), K53 (= K50), L115 (≠ S113), G116 (≠ A114), A144 (≠ T143), G145 (= G144), I185 (= I184), G311 (= G309), D312 (= D310), M318 (≠ P316), L319 (≠ F317), A320 (≠ T318), H321 (= H319)

Sites not aligning to the query:

active site: 467, 468

P0A9P0 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein; EC 1.8.1.4 from Escherichia coli (strain K12) (see 2 papers)
30% identity, 97% coverage: 7:450/458 of query aligns to 10:452/474 of P0A9P0

query
sites
P0A9P0

I

V

V

V

L

L

G

G

A

A

G

G

Q

P

S

A

G

G

M

Y

P

S

L

A

A

A

K

F

K

R

I

C

S

A

K

D

L

L

G

G

L

L

S

E

V

T

A

V

L

I

I

V

E

E

K

R

-

Y

R

N

V

T

I

L

G

G

T

V

C

C

I

L

N

N

D

V

G

G

C

C

S

I

P

P

T

S

K

K

T

A

M

L

V

L

S

H

S

V

A

A

R

K

V

V

A

I

H

E

I

E

V

A

S

K

R

A

A

L

A

A

D

E

F

H

G

G

T

I

I

V

L

F

P

G

H

E

Y

P

R

K

I

T

D

D

Q

I

R

D

I

K

V

I

K

R

K

T

R

W

K

K

D

E

H

K

I

V

V

I

E

N

L

Q

F

L

R

T

G

G

A

L

E

-

K

A

S

G

L

M

R

A

K

K

N

G

E

R

S

K

I

V

D

K

I

V

L

V

M

N

G

G

S

L

A

G

A

K

F

F

K

T

D

G

S

A

R

N

T

T

I

L

Q

E

I

V

T

E

S

G

D

E

T

N

G

G

E

K

I

-

S

T

F

V

I

I

S

N

G

F

T

D

K

N

I

A

F

I

I

I

N

A

T

A

G

G

S

S

E

R

P

P

-

I

R

Q

I

L

P

P

E

F

I

I

E

P

G

-

L

H

A

E

D

D

T

P

P

R

Y

I

L

W

T

D

S

S

T

T

T

D

I

A

M

L

E

E

L

L

E

K

E

E

T

V

P

P

E

E

H

R

L

L

I

V

M

M

G

G

Y

I

I

I

G

G

L

L

E

E

F

M

A

G

Q

T

M

V

F

Y

S

H

R

A

F

L

G

G

S

S

K

Q

V

I

T

D

I

V

D

E

R

M

A

F

E

D

R

Q

L

V

V

I

H

P

K

A

E

A

D

D

E

K

D

D

V

I

C

V

K
|
K

E

V

I

F

S

T

Q

K

I

R

F

I

S

S

E

K

G

-

I

F

D

N

T

L

L

M

F

L

N

E

A

T

E

K

V

V

R

T

K

A

V

V

-

E

S

A

Y

K

Q

E

D

D

G

G

F

I

K

Y

L

V

T

T

T

M

E

E

T

G

P

K

K

K

G

A

L

P

L

A

D

E

I

P

-

Q

K

R

G

Y

S

D

H

A

L

V

L

L

V

V

A

A

T

I

G

G

R

R

I

V

P

P

S

N

S

G

A

K

H

N

L

L

G

D

L

A

E

G

N

K

T

A

R

G

V

V

K

E

L

V

T

D

D

D

K

R

G

G

F

F

I

I

Q

R

T

V

D

D

D

K

F

Q

L

L

Q

R

T

T

S

N

E

V

K

P

H

H

I

I

Y

F

A

A

L

I

G

G

D

D

V

I

A

V

G

G

S

Q

A

P

P

M

F

L

T

A

H

H

I

K

A

G

Y

V

H

H

D

E

A

G

H

H

I

V

A

A

F

A

Q

E

H

V

A

I

F

-

K

-

E

-

N

-

A

A

V

G

S

K

K

K

H

H

-

Y

-

F

-

D

E

P

R

K

L

V

V

I

P

P

Y

S

C

I

V

A

F

Y

I

T

D

E

P

P

Q

E

L

V

G

A

R

W

I

V

G

G

L

L

N

T

E

E

Q

K

E

E

A

A

Q

K

A

E

K

K

G

G

I

I

P

S

Y

Y

K

E

T

T

G

A

K

T

F

F

L

P

M

W

K

A

H

A

A

S

G

G

R

R

T

A

L

I

E

A

V

S

D

D

E

C

T

A

R

D

G

G

F

M

F

T

K

K

V

L

Q

I

V

F

D

D

P

K

E

E

S

S

K

H

K

R

I

V

L

I

G

G

A

G

T

A

I

I

L

V

S

G

L

T

D

N

G

G

E

E

I

L

L

L

A

G

T

E

L

I

Q

G

M

L

A

A

M

I

V

E

G

M

G

G

V

C

T

D

Y

A

D

E

T

D

I

I

A

A

T

L

L

T

P

I

I

H

A

A

H

H

P

P

T

T

L

L

A

H

E

E

S

S

L

V

K220 (= K218) modified: N6-acetyllysine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1ebdA Dihydrolipoamide dehydrogenase complexed with the binding domain of the dihydrolipoamide acetylase (see paper)
31% identity, 97% coverage: 5:448/458 of query aligns to 5:445/455 of 1ebdA

query
sites
1ebdA

D

E

A

T

I

L

V

V

L

V

G
|
G

A

A

G
|
G

Q
x
P

S

G

G

G

M

Y

P

V

L

A

A

A

K

I

K

R

I

A

S

A

K

Q

L

L

G

G

L

Q

S

K

V

V

A

T

L

I

I
x
V

E
|
E

K
|
K

R

G

V

N

I
x
L

G

G

G
|
G

T
x
V

C
|
C

I

L

N

N

D

V

G
|
G

C
|
C

S

I

P

P

T
x
S

K
|
K

T

A

M

L

V

I

S

S

S

A

A

S

R

H

V

R

A

Y

H

E

I

Q

V

A

S

K

R

H

A

S

A

E

D

E

F

M

G

G

T

I

I

K

L

A

P

E

H
x
N

Y
x
V

R

T

I

I

D

D

Q

F

R

A

I

K

V

V

K

Q

K

E

R

W

K

K

D

A

H

S

I

V

V

V

E

K

L

K

F

L

R

T

G

G

A

V

E

E

K

G

S

L

L

L

R

K

K

G

N

N

E

K

S

-

I

V

D

E

I

I

L

V

M

K

G

G

S
x
E

A
|
A

A

Y

F

F

K

V

D

D

S

A

R

N

T

T

I

V

Q

R

I

V

T

V

S

N

D

G

T

D

G

S

E

A

I

Q

S

T

F

Y

I

T

S

F

G

K

T

N

K

A

I

I

F

-

I

I

N

A

T
|
T

G
|
G

S

S

E

R

P

P

-

I

R

E

I

L

P

P

E

N

I

F

E

K

G

-

L

-

A

F

D

S

T

N

P

R

Y

I

L

L

T

D

S

S

T

T

T

G

I

A

M

L

E

N

L

L

E

G

E

E

T

V

P

P

E

K

H

S

L

L

L

V

I

V

M

I

G

G

Y
|
Y

I
|
I

G

G

L

I

E
|
E

F

L

A

G

Q

T

M

A

F

Y

S

A

R

N

F

F

G

G

S

T

K

K

V

V

T

T

I

I

I

L

D

E

R

G

A

A

E

G

R

E

L

I

V

L

H

S

K

G

E

F

D

E

E

K

D

Q

V

M

C

A

K

A

E

I

I

I

S

K

Q

K

I

R

F

L

S

K

E

K

G

G

I

V

D

E

T

V

L

V

F

T

N

N

A

A

E

L

V

A

R

K

K

G

V

A

S

E

-

E

Y

R

Q

E

D

D

G

G

F

V

K

T

L

V

T

T

T

Y

E

E

T

A

P

N

K

G

G

E

L

T

L

K

D

T

I

I

K

D

G

A

S

D

H

Y

L

V

L

L

V

V

A

T

T

V

G

G

R
|
R

I

R

P

P

S

N

S

T

A

D

H

E

L

L

G

G

L

L

E

E

N

Q

T

I

R

G

V

I

K

K

L

M

T

T

D

N

K

R

G

G

F

L

I

I

Q

E

T

V

D

D

D

Q

F

Q

L

C

Q

R

T

T

S

S

E

V

K

P

H

N

I

I

Y

F

A

A

L

I

G

G

D
|
D

V

I

A

V

G

P

S

G

A

P

P
x
A

F
x
L

T
x
A

H

H

I

K

A

A

Y
x
S

H

Y

D

E

A

G

H

K

I

V

A

A

F

A

Q

E

H

-

A

-

F

-

K

-

E

-

N

-

A

A

V

I

S

A

K

G

H

H

E

P

R

S

L

A

V

V

-

D

-

Y

-

V

-

A

-

I

P

P

Y

A

C

V

V

V

F

F

I

S

D

D

P

P

Q

E

L

C

G

A

R

S

I

V

G

G

L

Y

N

F

E

E

Q

Q

E

Q

A

A

Q

K

A

D

K

E

G

G

I

I

P

D

Y

V

K

I

T

A

G

A

K

K

F

F

L

P

M

F

K

A

H

A

A

N

G

G

R

R

T

A

L

L

E

A

V

L

D

N

E

D

T

T

R

D

G

G

F

F

F

L

K

K

V

L

Q

V

V

V

D

R

P

K

E

E

S

D

K

G

K

V

I

I

L

I

G

G

A

A

T

Q

I

I

L

I

S

G

L

P

D

N

G

A

G

S

E

D

I

M

L

I

A

A

T

E

L

L

Q

G

M

L

A

A

M

I

V

E

G

A

G

G

V

M

T

T

Y

A

D

E

T

D

I

I

A

A

T

L

L

T

P

I

I
x
H

A

A

H
|
H

P

P

T

T

L

L

A

G

E
|
E

active site: P13 (≠ Q13), L37 (≠ I37), C41 (= C41), C46 (= C46), S49 (≠ T49), N74 (≠ H74), V75 (≠ Y75), Y180 (= Y183), E184 (= E187), S320 (≠ Y322), H438 (≠ I441), H440 (= H443), E445 (= E448)
binding flavin-adenine dinucleotide: G10 (= G10), G12 (= G12), P13 (≠ Q13), V32 (≠ I32), E33 (= E33), K34 (= K34), G39 (= G39), V40 (≠ T40), C41 (= C41), G45 (= G45), C46 (= C46), K50 (= K50), E112 (≠ S113), A113 (= A114), T141 (= T143), G142 (= G144), Y180 (= Y183), I181 (= I184), R268 (= R270), D308 (= D310), A314 (≠ P316), L315 (≠ F317), A316 (≠ T318)

P11959 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
31% identity, 97% coverage: 5:448/458 of query aligns to 11:451/470 of P11959

query
sites
P11959

D

E

A

T

I

L

V

V

L

V

G

G

A

A

G

G

Q

P

S

G

G

G

M

Y

P

V

L

A

A

A

K

I

K

R

I

A

S

A

K

Q

L

L

G

G

L

Q

S

K

V

V

A

T

L

I

I

V

E
|
E

K
|
K

R
x
G

V
x
N

I
x
L

G
|
G

T
x
V

C
|
C

I

L

N

N

D

V

G

G

C

C

S

I

P

P

T

S

K
|
K

T

A

M

L

V

I

S

S

S

A

A

S

R

H

V

R

A

Y

H

E

I

Q

V

A

S

K

R

H

A

S

A

E

D

E

F

M

G

G

T

I

I

K

L

A

P

E

H

N

Y

V

R

T

I

I

D

D

Q

F

R

A

I

K

V

V

K

Q

K

E

R

W

K

K

D

A

H

S

I

V

V

V

E

K

L

K

F

L

R

T

G

G

A

V

E

E

K

G

S

L

L

L

R

K

K

G

N

N

E

K

S

-

I

V

D

E

I

I

L

V

M

K

G

G

S

E

A

A

A

Y

F

F

K

V

D

D

S

A

R

N

T

T

I

V

Q

R

I

V

T

V

S

N

D

G

T

D

G

S

E

A

I

Q

S

T

F

Y

I

T

S

F

G

K

T

N

K

A

I

I

F

-

I

I

N

A

T

T

G

G

S

S

E

R

P

P

-

I

R

E

I

L

P

P

E

N

I

F

E

K

G

-

L

-

A

F

D

S

T

N

P

R

Y

I

L

L

T

D

S

S

T

T

T

G

I

A

M

L

E

N

L

L

E

G

E

E

T

V

P

P

E

K

H

S

L

L

L

V

I

V

M

I

G

G

Y

Y

I

I

G

G

L

I

E

E

F

L

A

G

Q

T

M

A

F

Y

S

A

R

N

F

F

G

G

S

T

K

K

V

V

T

T

I

I

I

L

D

E

R

G

A

A

E

G

R

E

L

I

V

L

H

S

K

G

E

F

D

E

E

K

D

Q

V

M

C

A

K

A

E

I

I

I

S

K

Q

K

I

R

F

L

S

K

E

K

G

G

I

V

D

E

T

V

L

V

F

T

N

N

A

A

E

L

V

A

R

K

K

G

V

A

S

E

-

E

Y

R

Q

E

D

D

G

G

F

V

K

T

L

V

T

T

T

Y

E

E

T

A

P

N

K

G

G

E

L

T

L

K

D

T

I

I

K

D

G

A

S

D

H

Y

L

V

L

L

V

V

A

T

T

V

G

G

R

R

I

R

P

P

S

N

S

T

A

D

H

E

L

L

G

G

L

L

E

E

N

Q

T

I

R

G

V

I

K

K

L

M

T

T

D

N

K

R

G

G

F

L

I

I

Q

E

T

V

D

D

D

Q

F

Q

L

C

Q

R

T

T

S

S

E

V

K

P

H

N

I

I

Y

F

A

A

L

I

G

G

D
|
D

V

I

A

V

G

P

S

G

A

P

P

A

F

L

T
x
A

H

H

I

K

A

A

Y

S

H

Y

D

E

A

G

H

K

I

V

A

A

F

A

Q

E

H

-

A

-

F

-

K

-

E

-

N

-

A

A

V

I

S

A

K

G

H

H

E

P

R

S

L

A

V

V

-

D

-

Y

-

V

-

A

-

I

P

P

Y

A

C

V

V

V

F

F

I

S

D

D

P

P

Q

E

L

C

G

A

R

S

I

V

G

G

L

Y

N

F

E

E

Q

Q

E

Q

A

A

Q

K

A

D

K

E

G

G

I

I

P

D

Y

V

K

I

T

A

G

A

K

K

F

F

L

P

M

F

K

A

H

A

A

N

G

G

R

R

T

A

L

L

E

A

V

L

D

N

E

D

T

T

R

D

G

G

F

F

F

L

K

K

V

L

Q

V

V

V

D

R

P

K

E

E

S

D

K

G

K

V

I

I

L

I

G

G

A

A

T

Q

I

I

L

I

S

G

L

P

D

N

G

A

G

S

E

D

I

M

L

I

A

A

T

E

L

L

Q

G

M

L

A

A

M

I

V

E

G

A

G

G

V

M

T

T

Y

A

D

E

T

D

I

I

A

A

T

L

L

T

P

I

I

H

A

A

H

H

P

P

T

T

L

L

A

G

E

E

39:47 (vs. 33:41, 56% identical) binding
K56 (= K50) binding
D314 (= D310) binding
A322 (≠ T318) binding

3urhB Crystal structure of a dihydrolipoamide dehydrogenase from sinorhizobium meliloti 1021
31% identity, 98% coverage: 4:451/458 of query aligns to 1:453/465 of 3urhB

query
sites
3urhB

Y

Y

D

D

A

L

I

I

V

V

L
x
I

G
|
G

A

S

G
|
G

Q
x
P

S
x
G

G

G

M

Y

P

V

L

C

A

A

K

I

K

K

I

A

S

A

K

Q

L

L

G

G

L

M

S

K

V

V

A

A

L

V

I

V

E
|
E

K
|
K

R

R

-

S

V

T

I
x
Y

G

G

G
|
G

T
|
T

C
|
C

I

L

N

N

D

V

G
|
G

C
|
C

S

I

P

P

T
x
S

K
|
K

T

A

M

L

V

L

S

H

S

A

A

S

R

E

V

M

A

F

H

H

I

Q

V

A

S

Q

R

H

A

G

A

L

D

E

-

A

F

L

G

G

T

V

I

E

L

V

P

A

H

N

Y

P

R

K

I

L

D

N

Q

L

R

Q

I

K

V

M

K

M

K

A

R

H

K

K

D

D

H

A

I

T

V

V

E

K

L

S

F

N

R

V

G

D

G

G

A

V

E

S

K

F

S

L

L

F

R

K

K

K

N

N

E

K

S

-

I

I

D

D

I

G

L

F

M

Q

G

G

S
x
T

A
x
G

A

K

F

V

K

L

D

G

S

Q

R

G

T

K

I

V

Q

S

I

V

T

T

S

N

D

E

T

K

G

G

E

E

I

E

S

Q

F

V

I

L

S

E

G

A

T

K

K

N

I

V

F

V

I

I

N
x
A

T
|
T

G
|
G

S

S

E

D

-

V

P

A

R

G

I

I

P

P

E

G

I

V

E

E

G

V

L

A

A

F

D

D

T

E

P

K

Y

T

L

I

T

V

S

S

T

S

T

T

-

G

I

A

M

L

E

A

L

L

E

E

E

K

T

V

P

P

E

A

H

S

L

M

L

I

I

V

M

V

G

G

Y
x
V

I
|
I

G

G

L

L

E
|
E

F

L

A

G

Q

S

M

V

F

W

S

A

R

R

F

L

G

G

S

A

K

K

V

V

T

T

I

V

D

E

R

F

A

L

E

D

R

T

L

I

V

L

H

G

K

G

E

M

D

D

E

G

D

E

V

V

C

A

K

K

E

Q

I

L

S

Q

Q

R

I

M

F

L

S

T

E

K

E

Q

G

G

I

I

D

D

T

F

L

K

F

L

N

G

A

A

E

K

V

V

R

T

-

G

K

A

V

V

S

K

Y

S

Q

G

D

D

G

G

F

A

K

K

L

V

T

T

T

F

E

E

T

P

P

V

K

K

G

G

-

G

-

E

L

A

L

T

D

T

I

L

K

D

G

A

S

E

H

V

L

V

L

L

V

I

A

A

T

T

G

G

R
|
R

I

K

P

P

S

S

S

T

A

D

H

G

L

L

G

G

L

L

E

A

N

K

T

A

R

G

V

V

K

V

L

L

T

D

D

S

K

R

G

G

F

R

I

V

Q

E

T

I

D

D

D

R

F

H

L

F

Q

Q

T

T

S

S

E

I

K

A

H

G

I

V

Y

Y

A

A

L

I

G
|
G

D
|
D

V

V

A

V

G

R

S

G

A

P

P
x
M

F
x
L

T
x
A

H
|
H

I

K

A

A

Y

-

H

E

D

D

A

E

H

G

I

V

A

A

F

V

Q

A

H

E

A

I

F

I

K

A

E

G

N

Q

A

A

V

G

S

H

K

V

H

N

E

Y

R

D

L

V

V

I

P

P

Y

G

C

V

V

V

F

Y

I

T

D

Q

P

P

Q

E

L

V

G

A

R

S

I

V

G

G

L

K

N

T

E

E

Q

E

E

E

A

L

Q

K

A

A

K

A

G

G

I

V

P

A

Y

Y

K

K

T

I

G

G

K

K

F

F

L

P

M

F

K

T

H

A

A

N

G

G

R

R

T

A

L

R

E

A

V

M

D

L

E

Q

T

T

R

D

G

G

F

F

F

V

K

K

V

I

Q

L

V

A

D

D

P

K

E

E

S

T

K

D

K

R

I

V

L

L

G

G

A

G

T

H

I

I

L

I

S

G

L

F

D

G

G

A

G

G

E

E

I

M

L

I

A

H

T

E

L

I

Q

A

M

V

A

L

M

M

V

E

G

F

G

G

V

G

T

S

Y

S

D

E

T

D

I

L

A

G

T

R

L

T

P

C

I
x
H

A

A

H
|
H

P

P

T

T

L

M

A

S

E
|
E

S

A

L

V

N

K

active site: Y35 (≠ I37), C39 (= C41), C44 (= C46), S47 (≠ T49), V183 (≠ Y183), E187 (= E187), H443 (≠ I441), H445 (= H443), E450 (= E448)
binding flavin-adenine dinucleotide: I6 (≠ L9), G7 (= G10), G9 (= G12), P10 (≠ Q13), G11 (≠ S14), E30 (= E33), K31 (= K34), G37 (= G39), T38 (= T40), C39 (= C41), G43 (= G45), C44 (= C46), K48 (= K50), T111 (≠ S113), G112 (≠ A114), A140 (≠ N142), T141 (= T143), G142 (= G144), I184 (= I184), R273 (= R270), G312 (= G309), D313 (= D310), M319 (≠ P316), L320 (≠ F317), A321 (≠ T318), H322 (= H319)

6uziC Crystal structure of dihydrolipoyl dehydrogenase from elizabethkingia anophelis nuhp1
30% identity, 98% coverage: 1:450/458 of query aligns to 4:455/470 of 6uziC

query
sites
6uziC

M

M

E

N

N

Q

Y

F

D

D

A

V

I

A

V

V

L
x
I

G
|
G

A

S

G
|
G

Q
x
P

S
x
G

G

G

M

Y

P

V

L

A

A

A

K

I

K

R

I

C

S

A

K

Q

L

L

G

G

L

F

S

K

V

T

A

V

L

I

I

I

E
|
E

K
|
K

-

Y

R

S

V

T

I

L

G

G

G
|
G

T
|
T

C
|
C

I

L

N

N

D

V

G
|
G

C
|
C

S

I

P

P

T
x
S

K
|
K

T

A

M

L

V

L

-

D

S

S

A

E

R

H

V

F

A

E

H

N

I

A

V

K

S

H

R

T

A

F

A

A

D

T

F

H

G

G

T

I

I

L

L

I

P

D

H

E

Y

P

R

K

I

V

D

D

Q

I

R

A

I

Q

V

M

K

I

K

S

R

R

K

K

D

N

H

D

I

V

V

V

E

D

L

Q

F

T

R

T

G

K

G

G

A

I

E

N

K

F

S

L

L

M

R

D

K

K

N

N

E

K

S

-

I

I

D

T

I

V

L

L

M

Q

G

G

S
x
V

A
x
G

A

S

F

F

K

E

D

S

S

A

R

T

T

Q

I

I

Q

K

I

V

T

T

S

K

D

A

T

D

G

G

E

S

I

S

S

E

F

V

I

I

S

E

G

A

T

K

K

N

I

T

F

I

I

I

N

A

T
|
T

G
|
G

S

S

E

K

P

P

R

S

I

S

P

L

E

P

I

F

E

I

G

T

L

L

A

D

D

K

T

E

P

R

Y

V

L

I

T

T

S

S

T

T

T

E

I

A

M

L

E

N

L

L

E

K

E

E

T

V

P

P

E

K

H

H

L

L

L

I

I

V

M

I

G

G

Y
x
V

I
|
I

G

G

L

L

E
|
E

F

L

A

G

Q

S

M

V

F

Y

S

L

R

R

F

L

G

G

S

S

K

D

V

V

T

T

I

V

D

E

R

Y

A

L

E

D

R

K

L

I

V

I

H

P

K

G

E

M

D

D

E

G

D

T

V

L

C

S

K

K

E

E

I

L

S

Q

Q

K

I

T

F

L

S

K

E

K

E

Q

G

G

I

M

D

K

T

F

L

M

F

L

N

S

A

T

E

A

V

V

R

S

K

G

V

V

S

E

Y

R

Q

N

-

G

D

D

G

T

F

V

K

K

L

V

T

T

T

A

E

K

T

D

P

K

K

K

G

G

L

E

-

D

L

V

D

V

I

V

K

E

G

G

S

D

H

Y

L

C

L

L

V

V

A

S

T

V

G

G

R
|
R

I

R

P

P

S

Y

S

T

A

D

H

G

L

L

G

G

L

L

E

E

N

K

T

A

R

G

V

V

K

E

L

L

T

D

D

E

K

R

G

G

F

R

I

V

Q

K

T

T

D

N

D

D

F

H

L

L

Q

Q

T

T

S

N

E

V

K

P

H

N

I

I

Y

Y

A

A

L

I

G

G

D
|
D

V

V

A

V

G

K

S

G

A

A

P
x
M

F
x
L

T
x
A

H

H

I

K

A

A

Y

E

H

E

D

E

A

G

H

V

I

F

A

V

F

A

Q

E

H

T

A

L

F

A

K

G

E

E

N

K

A

P

V

H

S

V

K

N

H

Y

E

-

R

N

L

L

V

I

P

P

Y

G

C

V

V

V

F

Y

I

T

D

W

P

P

Q

E

L

V

G

A

R

G

I

V

G

G

L

K

N

T

E

E

Q

E

E

Q

A

L

Q

K

A

E

K

A

G

G

I

V

P

A

Y

Y

K

K

T

T

G

G

K

S

F

F

L

P

M

M

K

R

H

A

A

L

G

G

R

R

T

S

L

R

E

A

V

S

D

M

E

D

T

T

R

D

G

G

F

V

F

I

K

K

V

I

Q

L

V

A

D

D

P

E

E

K

S

T

K

D

K

E

I

I

L

L

G

G

A

V

T

H

I

M

L

I

S

G

L

A

D

R

G

A

E

D

I

M

L

I

A

A

T

E

L

A

Q

V

M

V

A

A

M

M

V

E

G

F

G

R

V

A

T

S

Y

A

D

E

T

D

I

I

A

A

T

R

L

I

P

S

I

H

A

A

H
|
H

P

P

T

T

L

Y

A

T

E
|
E

S

A

L

I

active site: C45 (= C41), C50 (= C46), S53 (≠ T49), V187 (≠ Y183), E191 (= E187), H448 (= H443), E453 (= E448)
binding flavin-adenine dinucleotide: I12 (≠ L9), G13 (= G10), G15 (= G12), P16 (≠ Q13), G17 (≠ S14), E36 (= E33), K37 (= K34), G43 (= G39), T44 (= T40), C45 (= C41), G49 (= G45), C50 (= C46), S53 (≠ T49), K54 (= K50), V117 (≠ S113), G118 (≠ A114), T147 (= T143), G148 (= G144), I188 (= I184), R276 (= R270), D316 (= D310), M322 (≠ P316), L323 (≠ F317), A324 (≠ T318)
binding zinc ion: H448 (= H443), E453 (= E448)

P00392 Mercuric reductase; Hg(II) reductase; EC 1.16.1.1 from Pseudomonas aeruginosa (see 2 papers)
31% identity, 97% coverage: 8:450/458 of query aligns to 103:542/561 of P00392

query
sites
P00392

V

V

L

I

G

G

A

S

G

G

Q

G

S

A

G
x
A

M

M

P

A

L

A

A

A

K

L

K

K

I

A

S

V

K

E

L

Q

G

G

L

A

S

Q

V

V

A

T

L

L

I

I

E

E

K

R

R
x
G

V

T

I

I

G

G

T
|
T

C
|
C

I

V

N

N

D

V

G

G

C
|
C

S

V

P

P

T

S

K
|
K

T

I

M

M

V

I

S

R

S

A

A

A

R

H

V

I

A

A

H

H

I

L

V

R

S

R

R

E

A

S

A

P

-

F

D

D

F

G

G

G

T

I

I

A

L

A

P

T

H

V

Y

P

R

T

I

I

D

D

Q

R

-

S

R

K

I

L

V

L

K

A

K

Q

R

Q

K

Q

D

A

H

R

I

V

V

D

E

E

L

L

F

R

R

H

G

A

G

K

A

Y

E

E

K

G

S

I

L

L

R

G

K

G

N

N

E

P

S

A

I

I

D

T

I

V

L

V

M

H

G

G

S

E

A
|
A

A

R

F

F

K

K

D

D

S

D

R

Q

T

S

I

L

Q

T

I

V

T

R

S

L

D

N

T

E

G

G

E

G

I

E

S

R

F

V

I

V

S

M

G

F

T

D

K

R

I

C

F

L

I

V

N

A

T

T

G

G

S

A

E

S

P

P

R

A

I

V

P

P

E

P

I

I

E

P

G

G

L

L

A

K

D

E

T

S

P

P

Y

Y

L

W

T

T

S

S

T

T

T

E

I

A

M

L

E

A

L

S

E

D

E

T

T

I

P

P

E

E

H

R

L

L

L

A

I

V

M

I

G

G

G

S

Y

V

I

V

G

A

L

L

E

E

F

L

A

A

Q

Q

M

A

F

F

S

A

R

R

F

L

G

G

S

S

K

K

V

V

T

T

I

V

I

L

D

A

R

R

A

-

E

N

R

T

L

L

V

F

H

F

K

R

E

E

D

D

E

P

D

A

V

I

C

G

K

E

E

A

I

V

S

T

Q

A

I

A

F

F

S

R

E

A

E

E

G

G

I

I

D

E

T

V

L

L

F

E

N

H

A

T

E

Q

V

A

R

S

K

Q

V

V

S

A

Y

H

Q

M

D

D

G

G

-

E

F

F

K

V

L

L

T

T

E

T

T

H

P

G

K

-

G

-

L

-

D

E

I

L

K

R

G

A

S

D

H

K

L

L

V

V

A

A

T

T

G

G

R

R

I

T

P

P

S

N

S

T

A

R

H

S

L

L

G

A

L

L

E

D

N

A

T

A

R

G

V

V

K

T

L

V

T

N

D

A

K

Q

G

G

F

A

I

I

Q

V

T

I

D

D

D

Q

F

G

L

M

Q

R

T

T

S

S

E

N

K

P

H

N

I

I

Y

Y

A

A

L

A

G

G

D
|
D

V

C

A

T

G

D

S

Q

A

P

P

Q

F

F

T
x
V

H

Y

I

V

A

A

Y

A

H

A

D

A

A

G

H

T

I

R

A

A

F

A

Q

I

H

N

A

M

F

T

K

G

E

G

N

D

A

A

V

A

S

L

K

D

H

L

E

T

R

A

L

M

V

-

P

P

Y

A

C

V

V

V

F

F

I

T

D

D

P

P

Q

Q

L

V

G

A

R

T

I

V

G

G

L

Y

N

S

E

E

Q

A

E

E

A

A

Q

H

A

H

K

D

G

G

I

I

P

E

Y

T

K

D

T

S

G

R

K

T

F

L

L

T

M

L

K

D

H

N

A

V

G

P

R

R

T

A

L

L

E

A

V

N

D

F

E

D

T

T

R

R

G

G

F

F

F

I

K

K

V

L

Q

V

V

I

D

E

P

E

E

G

S

S

K

H

K

R

I

L

L

I

G

G

A

V

T

Q

I

A

L

V

S

A

L

P

D

E

G

A

G

G

E

E

I

L

L

I

A

Q

T

T

L

A

Q

A

M

L

A

A

M

I

V

R

G

N

G

R

V

M

T

T

Y

V

D

Q

T

E

I

L

A

A

T

D

L

Q

P

L

I

F

A

P

H

Y

P

L

T

T

L

M

A

V

E

E

S

G

L

L

A110 (≠ G15) binding
G130 (≠ R35) binding
T135 (= T40) binding
C136 (= C41) modified: Disulfide link with 141, Redox-active
C141 (= C46) modified: Disulfide link with 136, Redox-active
K145 (= K50) binding
A211 (= A114) binding
D403 (= D310) binding
V411 (≠ T318) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
558 binding
559 binding

4k8dA Crystal structure of the c558(464)a/c559(465)a double mutant of tn501 mera in complex with NADPH and hg2+
31% identity, 97% coverage: 8:450/458 of query aligns to 8:447/466 of 4k8dA

query
sites
4k8dA

V

V

L
x
I

G
|
G

A

S

G
|
G

Q
x
G

S
x
A

G

A

M

M

P

A

L

A

A

A

K

L

K

K

I

A

S

V

K

E

L

Q

G

G

L

A

S

Q

V

V

A

T

L

L

I

I

E
|
E

K
x
R

R

G

V

T

I
|
I

G

G

G
|
G

T
|
T

C
|
C

I

V

N

N

D

V

G
|
G

C
|
C

S

V

P

P

T
x
S

K
|
K

T

I

M

M

V

I

S

R

S

A

A

A

R

H

V

I

A

A

H

H

I

L

V

R

S

R

R

E

A

S

A

P

-

F

D

D

F

G

G

G

T

I

I

A

L

A

P

T

H
x
V

Y
x
P

R

T

I

I

D

D

Q

R

-

S

R

K

I

L

V

L

K

A

K

Q

R

Q

K

Q

D

A

H

R

I

V

V

D

E

E

L

L

F

R

R

H

G

A

G

K

A

Y

E

E

K

G

S

I

L

L

R

G

K

G

N

N

E

P

S

A

I

I

D

T

I

V

L

V

M

H

G

G

S
x
E

A
|
A

A

R

F

F

K

K

D

D

S

D

R

Q

T

S

I

L

Q

T

I

V

T

R

S

L

D

N

T

E

G

G

E

G

I

E

S

R

F

V

I

V

S

M

G

F

T

D

K

R

I

C

F

L

I

V

N

A

T
|
T

G
|
G

S

A

E

S

P

P

R

A

I

V

P

P

E

P

I

I

E

P

G

G

L

L

A

K

D

E

T

S

P

P

Y

Y

L

W

T

T

S

S

T

T

T

E

I

A

M

L

E

A

L

S

E

D

E

T

T

I

P

P

E

E

H

R

L

L

L

A

I

V

M

I

G

G

G
x
S

Y
x
V

I
x
V

G

A

L

L

E
|
E

F

L

A

A

Q

Q

M

A

F

F

S

A

R

R

F

L

G

G

S

S

K

K

V

V

T

T

I

V

I

L

D

A

R
|
R

A

-

E
x
N

R

T

L

L

V

F

H

F

K
x
R

E

E

D

D

E

P

D

A

V

I

C

G

K

E

E

A

I

V

S

T

Q

A

I

A

F

F

S

R

E

A

E

E

G

G

I

I

D

E

T

V

L

L

F

E

N

H

A

T

E

Q

V

A

R

S

K

Q

V

V

S

A

Y

H

Q

M

D

D

G

G

-

E

F

F

K

V

L

L

T

T

E

T

T

H

P

G

K

-

G

-

L

-

D

E

I

L

K

R

G

A

S

D

H

K

L

L

V

V

A

A

T
|
T

G
|
G

R
|
R

I

T

P

P

S

N

S

T

A

R

H

S

L

L

G

A

L

L

E

D

N

A

T

A

R

G

V

V

K

T

L

V

T

N

D

A

K

Q

G

G

F

A

I

I

Q

V

T

I

D

D

D

Q

F

G

L

M

Q

R

T

T

S

S

E

N

K

P

H

N

I

I

Y

Y

A

A

L

A

G
|
G

D
|
D

V

C

A

T

G

D

S

Q

A

P

P
x
Q

F
|
F

T
x
V

H
x
Y

I

V

A

A

Y
x
A

H

A

D

A

A

G

H

T

I

R

A

A

F

A

Q

I

H

N

A

M

F

T

K

G

E

G

N

D

A

A

V

A

S

L

K

D

H

L

E

T

R

A

L

M

V

-

P

P

Y

A

C
x
V

V

V

F

F

I

T

D

D

P

P

Q

Q

L

V

G

A

R

T

I

V

G

G

L

Y

N

S

E

E

Q

A

E

E

A

A

Q

H

A

H

K

D

G

G

I

I

P

E

Y

T

K

D

T

S

G

R

K

T

F

L

L

T

M

L

K

D

H

N

A

V

G

P

R

R

T

A

L

L

E

A

V

N

D

F

E

D

T

T

R

R

G

G

F

F

F

I

K

K

V

L

Q

V

V

I

D

E

P

E

E

G

S

S

K

H

K

R

I

L

L

I

G

G

A

V

T

Q

I

A

L

V

S

A

L

P

D

E

G

A

G

G

E

E

I

L

L

I

A

Q

T

T

L

A

Q

A

M

L

A

A

M

I

V

R

G

N

G

R

V

M

T

T

Y

V

D

Q

T

E

I

L

A

A

T

D

L

Q

P

L

I
x
F

A

P

H
x
Y

P

L

T

T

L

M

A

V

E
|
E

S

G

L

L

active site: G13 (≠ Q13), I37 (= I37), C41 (= C41), C46 (= C46), S49 (≠ T49), V75 (≠ H74), P76 (≠ Y75), V185 (≠ Y183), E189 (= E187), A320 (≠ Y322), F438 (≠ I441), Y440 (≠ H443), E445 (= E448)
binding flavin-adenine dinucleotide: I9 (≠ L9), G10 (= G10), G12 (= G12), A14 (≠ S14), E33 (= E33), R34 (≠ K34), G39 (= G39), T40 (= T40), C41 (= C41), G45 (= G45), C46 (= C46), K50 (= K50), E115 (≠ S113), A116 (= A114), T145 (= T143), G146 (= G144), R268 (= R270), G307 (= G309), D308 (= D310), F315 (= F317), V316 (≠ T318), Y317 (≠ H319)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: S183 (≠ G181), S184 (≠ G182), V185 (≠ Y183), V186 (≠ I184), E189 (= E187), R206 (= R204), N207 (≠ E206), R212 (≠ K211), T266 (= T268), G267 (= G269), Q314 (≠ P316), F315 (= F317), V345 (≠ C348)

Sites not aligning to the query:

active site: 463, 464

3o0hB Crystal structure of glutathione reductase from bartonella henselae
30% identity, 98% coverage: 3:450/458 of query aligns to 3:442/459 of 3o0hB

query
sites
3o0hB

N

D

Y

F

D

D

A

L

I

F

V

V

L
x
I

G
|
G

A

S

G
|
G

Q
x
S

S
x
G

G

G

M

V

P

R

L

A

A

A

K

R

K

L

I

A

S

G

K

A

L

L

G

G

L

K

S

R

V

V

A

A

L

I

I
x
A

E
|
E

K
x
E

R

Y

V

R

I
|
I

G

G

T
|
T

C
|
C

I

V

N

I

D

R

G
|
G

C
|
C

S

V

P

P

T
x
K

K

K

T

L

M

Y

V

F

S

Y

S

A

A

S

R

Q

V

Y

A

A

H

Q

I

E

V

F

S

S

R

K

A

S

A

I

D

G

F

F

G

G

T

W

I

K

L

Y

-

A

-
x
D

P
|
P

H

I

Y

F

R

N

I

W

D

E

Q

K

R

L

I

V

V

A

K

A

K

K

R

N

K

K

D

E

-

I

-

S

H

R

I

L

V

E

E

G

L

L

F

Y

R

R

G

E

G

G

A

L

E

Q

K

-

S

-

L

-

R

-

K

-

N

N

E

S

S

N

I

V

D

H

I

I

L

Y

M

E

G

S

S
x
R

A
|
A

A

V

F

F

K

V

D

D

S

E

R

H

T

T

I

L

Q

E

I

L

T

-

S

S

D

V

T

T

G

G

E

E

I

-

S

-

F

R

I

I

S

S

G

A

T

E

K

K

I

I

F

L

I

I

N

A

T
|
T

G
|
G

S

A

E

K

P

I

R

V

I

S

P

N

E

S

I

I

E

K

G

G

L

-

A

-

D

S

T

D

P

L

Y

C

L

L

T

T

S

S

T

N

T

E

I

I

M

F

E

D

L

L

E

E

E

K

T

L

P

P

E

K

H

S

L

I

L

V

I

I

M

V

G

G

Y
|
Y

I

I

G

G

L

V

E
|
E

F

F

A

A

Q

N

M

I

F

F

S

H

R

G

F

L

G

G

S

V

K

K

V

T

T

T

I

L

D

H

R

R

A

G

E

D

R

L

L

I

V

L

H

R

K

N

E

F

D

D

E

Y

D

D

V

L

C

R

K

Q

E

L

I

L

S

N

Q

D

I

A

F

M

S

V

E

A

E

K

G

G

I

I

D

S

T

I

L

I

F

Y

N

E

A

A

E

T

V

V

R

S

K

Q

V

V

-

Q

S

S

Y

T

Q

E

D

N

G

C

F

Y

K

N

L

V

T

V

T

L

E

T

T

N

P

G

K

Q

G

-

L

-

D

T

I

I

K

C

G

A

S

D

H

R

L

V

L

M

V

L

A

A

T

T

G

G

R
|
R

I

V

P

P

S
x
N

S

T

A

T

H

G

L

L

G

G

L

L

E

E

N

R

T

A

R

G

V

V

K

K

L

V

T

N

D

E

K

F

G

G

F

A

I

V

Q

V

T

V

D

D

D

E

F

K

L

M

Q

T

T

T

S

N

E

V

K

S

H

H

I

I

Y

W

A

A

L

V

G
|
G

D
|
D

V

V

A

T

G

G

S

H

A

I

P
x
Q

F
x
L

T
|
T

H

P

I

V

A

A

Y
x
I

H

H

D

D

A

A

H

M

I

C

A

F

F

V

Q

K

H

N

A

A

F

F

K

E

E

N

N

T

A

S

V

T

S

T

K

P

H

D

E

Y

R

D

L

L

V

I

P

T

Y

T

C

A

V

V

F

F

I

S

D

Q

P

P

Q

E

L

I

G

G

R

T

I

V

G

G

L

L

N

S

E

E

Q

E

E

D

A

A

Q

L

A

H

K

R

G

Y

I

K

P

R

Y

V

K

E

T

I

G

Y

K

R

F

T

L

V

M

F

K

R

H

P

A

M

G

R

R

N

T

V

L

L

E

S

V

G

D

S

E

P

T

E

R

K

G

M

F

F

F

M

K

K

V

L

Q

V

V

V

D

D

P

G

E

E

S

S

K

R

K

I

I

V

L

V

G

G

A

A

T

H

I

V

L

L

S

G

L

E

D

N

G

A

G

G

E

E

I

I

L

A

A

Q

T

L

L

I

Q

G

M

I

A

S

M

L

V

K

G

G

G

K

V

L

T

T

Y

K

D

D

T

I

I

F

A

D

T

K

L

T

P

M

I
x
A

A

V

H
|
H

P

P

T

T

L

M

A

S

E
|
E

S

E

L

L

active site: S13 (≠ Q13), I37 (= I37), C41 (= C41), C46 (= C46), K49 (≠ T49), D74 (vs. gap), P75 (= P73), Y177 (= Y183), E181 (= E187), I314 (≠ Y322), A433 (≠ I441), H435 (= H443), E440 (= E448)
binding flavin-adenine dinucleotide: I9 (≠ L9), G10 (= G10), G12 (= G12), S13 (≠ Q13), G14 (≠ S14), A32 (≠ I32), E33 (= E33), E34 (≠ K34), T40 (= T40), C41 (= C41), G45 (= G45), C46 (= C46), K49 (≠ T49), R112 (≠ S113), A113 (= A114), T139 (= T143), G140 (= G144), Y177 (= Y183), R262 (= R270), N265 (≠ S273), G301 (= G309), D302 (= D310), Q308 (≠ P316), L309 (≠ F317), T310 (= T318)

Sites not aligning to the query:

active site: 459

2eq7A Crystal structure of lipoamide dehydrogenase from thermus thermophilus hb8 with psbdo
30% identity, 98% coverage: 4:452/458 of query aligns to 2:443/452 of 2eq7A

query
sites
2eq7A

Y

Y

D

D

A

L

I

L

V

V

L

I

G

G

A

A

G
|
G

Q
x
P

S
x
G

G

G

M

Y

P

V

L

A

A

A

K

I

K

R

I

A

S

A

K

Q

L

L

G

G

L

M

S

K

V

V

A

G

L

V

I

V

E
|
E

K
|
K

-

E

R

K

V

A

I
x
L

G

G

G
|
G

T
|
T

C
|
C

I

L

N
x
R

D

V

G
|
G

C
|
C

S

I

P

P

T
x
S

K
|
K

T

A

M

L

V

L

-

E

S

T

A

E

R

R

V

I

A

Y

H

E

I

-

V

-

S

A

R

K

A

K

A

G

D

L

F

L

G

G

T

A

I

K

L

V

P

K

H
x
G

Y
x
V

R

E

I

L

D

D

Q

L

R

P

I

A

V

L

K

M

K

A

R

H

K

K

D

D

H

K

I

V

V

V

E

Q

L

A

F

N

R

T

G

Q

G

G

A

V

E

E

K

F

S

L

L

F

R

K

K

K

N

N

E

-

S

G

I

I

D

A

I

R

L

H

M

Q

G

G

S
x
T

A
|
A

A

R

F

F

K

L

D

S

S

E

R

R

T

K

I

V

Q

-

I

L

T

V

S

E

D

E

T

T

G

G

E

E

I

-

S

-

F

E

I

L

S

E

G

A

T

R

K

Y

I

I

F

L

I

I

N

A

T
|
T

G
|
G

S

S

E

A

P

P

R

L

I

I

P

P

E

P

I
x
W

E

A

G

Q

L

V

A

D

D

Y

T

E

P

R

Y

V

L

V

T

T

S
|
S

T

T

T

E

I

A

M

L

E

S

L

F

E

P

E

E

T

V

P

P

E

K

H

R

L

L

L

I

I

V

M

V

G
|
G

G

G

G
|
G

Y
x
V

I
|
I

G

G

L

L

E
|
E

F

L

A

G

Q

V

M

V

F

W

S

H

R

R

F

L

G

G

S

A

K

E

V

V

T

I

I

V

I

L

D
x
E

R
x
Y

A

M

E

D

R

R

L

I

V

L

H

P

K

T

E

M

D

D

E

L

D

E

V

V

C

S

K

R

E

A

I

A

S

E

Q

R

I

V

F

F

S

K

E

K

E

Q

G

G

I

L

D

T

T

I

L

R

F

T

N

G

A

V

E

R

V
|
V

R

T

K

A

V

V

S

V

Y

P

Q

E

-

A

D

K

G

G

F

A

K

R

L

V

T

E

T

L

E

E

T

-

P

-

K

-

G

G

L

G

L

E

D

V

I

L

K

E

G

A

S

D

H

R

L

V

L

L

V

V

A

A

T
x
V

G
|
G

R
|
R

I

R

P

P

S
x
Y

S

T

A

E

H

G

L

L

G

S

L

L

E

E

N

N

T

A

R

G

V

L

K

S

L

T

T

D

D

E

K

R

G

G

F

R

I

I

Q

P

T

V

D

D

D

E

F

H

L

L

Q

R

T

T

S

R

E

V

K

P

H

H

I

I

Y

Y

A

A

L

I

G

G

D
|
D

V

V

A

V

G

R

S

G

A

P

P
x
M

F
x
L

T
x
A

H
|
H

I

K

A

A

Y
x
S

H

E

D

E

A

G

H

I

I

A

A

A

F

V

Q

E

H

H

A

M

F

V

K

R

E

G

N

F

A

G

V

H

S

V

K

D

H

Y

E

Q

R

-

L

A

V

I

P

P

Y

S

C
x
V

V

V

F
x
Y

I

T

D

H

P

P

Q

E

L

I

G

A

R

A

I

V

G

G

L

Y

N

T

E

E

Q

E

E

E

A

L

Q

K

A

A

K

Q

G

G

I

I

P

P

Y

Y

K

K

T

V

G

G

K

K

F

F

L

P

M

Y

K

S

H

A

A

S

G

G

R

R

T

A

L

R

E

A

V

M

D

G

E

E

T

T

R

E

G

G

F

F

F

I

K

K

V

V

Q

L

V

A

D

H

P

A

E

K

S

T

K

D

K

R

I

I

L

L

G

G

A

V

T

H

I

G

L

I

S

G

L

A

D

R

G

V

G

G

E

D

I

V

L

L

A

A

T

E

L

A

Q

A

M

L

A

A

M

L

V

F

G

F

G

K

V

A

T

S

Y

A

D

E

T

D

I

L

A

G

T

R

L

A

P

P

I
x
H

A

A

H
|
H

P

P

T

S

L

L

A

S

E
|
E

S

I

L

L

N

K

N

E

active site: P11 (≠ Q13), L36 (≠ I37), C40 (= C41), C45 (= C46), S48 (≠ T49), G72 (≠ H74), V73 (≠ Y75), V177 (≠ Y183), E181 (= E187), S314 (≠ Y322), H432 (≠ I441), H434 (= H443), E439 (= E448)
binding flavin-adenine dinucleotide: G10 (= G12), P11 (≠ Q13), G12 (≠ S14), E31 (= E33), K32 (= K34), G38 (= G39), T39 (= T40), C40 (= C41), R42 (≠ N43), G44 (= G45), C45 (= C46), K49 (= K50), T110 (≠ S113), A111 (= A114), T137 (= T143), G138 (= G144), S157 (= S163), I178 (= I184), R262 (= R270), Y265 (≠ S273), D302 (= D310), M308 (≠ P316), L309 (≠ F317), A310 (≠ T318), H311 (= H319), Y341 (≠ F350)
binding nicotinamide-adenine-dinucleotide: W146 (≠ I152), G174 (= G180), G176 (= G182), V177 (≠ Y183), I178 (= I184), E197 (≠ D203), Y198 (≠ R204), V231 (= V237), V260 (≠ T268), G261 (= G269), R262 (= R270), M308 (≠ P316), L309 (≠ F317), V339 (≠ C348)

2yquB Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(e3s) from thermus thermophilus
30% identity, 98% coverage: 4:452/458 of query aligns to 2:443/455 of 2yquB

query
sites
2yquB

Y

Y

D

D

A

L

I

L

V

V

L

I

G
|
G

A

A

G
|
G

Q
x
P

S
x
G

G

G

M

Y

P

V

L

A

A

A

K

I

K

R

I

A

S

A

K

Q

L

L

G

G

L

M

S

K

V

V

A

G

L

V

I

V

E
|
E

K
|
K

-

E

R

K

V

A

I
x
L

G

G

G
|
G

T
|
T

C
|
C

I

L

N
x
R

D

V

G
|
G

C
|
C

S

I

P

P

T
x
S

K
|
K

T

A

M

L

V

L

-

E

S

T

A

E

R

R

V

I

A

Y

H

E

I

-

V

-

S

A

R

K

A

K

A

G

D

L

F

L

G

G

T

A

I

K

L

V

P

K

H
x
G

Y
x
V

R

E

I

L

D

D

Q

L

R

P

I

A

V

L

K

M

K

A

R

H

K

K

D

D

H

K

I

V

V

V

E

Q

L

A

F

N

R

T

G

Q

G

G

A

V

E

E

K

F

S

L

L

F

R

K

K

K

N

N

E

-

S

G

I

I

D

A

I

R

L

H

M

Q

G

G

S
x
T

A
|
A

A

R

F

F

K

L

D

S

S

E

R

R

T

K

I

V

Q

-

I

L

T

V

S

E

D

E

T

T

G

G

E

E

I

-

S

-

F

E

I

L

S

E

G

A

T

R

K

Y

I

I

F

L

I

I

N

A

T
|
T

G
|
G

S

S

E

A

P

P

R

L

I

I

P

P

E

P

I

W

E

A

G

Q

L

V

A

D

D

Y

T

E

P

R

Y

V

L

V

T

T

S

S

T

T

T

E

I

A

M

L

E

S

L

F

E

P

E

E

T

V

P

P

E

K

H

R

L

L

L

I

I

V

M

V

G

G

Y
x
V

I
|
I

G

G

L

L

E
|
E

F

L

A

G

Q

V

M

V

F

W

S

H

R

R

F

L

G

G

S

A

K

E

V

V

T

I

I

V

I

L

D

E

R

Y

A

M

E

D

R

R

L

I

V

L

H

P

K

T

E

M

D

D

E

L

D

E

V

V

C

S

K

R

E

A

I

A

S

E

Q

R

I

V

F

F

S

K

E

K

E

Q

G

G

I

L

D

T

T

I

L

R

F

T

N

G

A

V

E

R

V

V

R

T

K

A

V

V

S

V

Y

P

Q

E

-

A

D

K

G

G

F

A

K

R

L

V

T

E

T

L

E

E

T

-

P

-

K

-

G

G

L

G

L

E

D

V

I

L

K

E

G

A

S

D

H

R

L

V

L

L

V

V

A

A

T

V

G

G

R

R

I

R

P

P

S
x
Y

S

T

A

E

H

G

L

L

G

S

L

L

E

E

N

N

T

A

R

G

V

L

K

S

L

T

T

D

D

E

K

R

G

G

F

R

I

I

Q

P

T

V

D

D

D

E

F

H

L

L

Q

R

T

T

S

R

E

V

K

P

H

H

I

I

Y

Y

A

A

L

I

G
|
G

D
|
D

V

V

A

V

G

R

S

G

A

P

P
x
M

F
x
L

T
x
A

H
|
H

I

K

A

A

Y
x
S

H

E

D

E

A

G

H

I

I

A

A

A

F

V

Q

E

H

H

A

M

F

V

K

R

E

G

N

F

A

G

V

H

S

V

K

D

H

Y

E

Q

R

-

L

A

V

I

P

P

Y

S

C

V

V

V

F

Y

I

T

D

H

P

P

Q

E

L

I

G

A

R

A

I

V

G

G

L

Y

N

T

E

E

Q

E

E

E

A

L

Q

K

A

A

K

Q

G

G

I

I

P

P

Y

Y

K

K

T

V

G

G

K

K

F

F

L

P

M

Y

K

S

H

A

A

S

G

G

R

R

T

A

L

R

E

A

V

M

D

G

E

E

T

T

R

E

G

G

F

F

F

I

K

K

V

V

Q

L

V

A

D

H

P

A

E

K

S

T

K

D

K

R

I

I

L

L

G

G

A

V

T

H

I

G

L

I

S

G

L

A

D

R

G

V

G

G

E

D

I

V

L

L

A

A

T

E

L

A

Q

A

M

L

A

A

M

L

V

F

G

F

G

K

V

A

T
x
S

Y

A

D

E

T
x
D

I

L

A

G

T

R

L

A

P

P

I
x
H

A

A

H
|
H

P

P

T

S

L

L

A

S

E
|
E

S

I

L

L

N

K

N

E

active site: P11 (≠ Q13), L36 (≠ I37), C40 (= C41), C45 (= C46), S48 (≠ T49), G72 (≠ H74), V73 (≠ Y75), V177 (≠ Y183), E181 (= E187), S314 (≠ Y322), H432 (≠ I441), H434 (= H443), E439 (= E448)
binding carbonate ion: A310 (≠ T318), S314 (≠ Y322), S423 (≠ T432), D426 (≠ T435)
binding flavin-adenine dinucleotide: G8 (= G10), G10 (= G12), P11 (≠ Q13), G12 (≠ S14), E31 (= E33), K32 (= K34), G38 (= G39), T39 (= T40), C40 (= C41), R42 (≠ N43), G44 (= G45), C45 (= C46), K49 (= K50), T110 (≠ S113), A111 (= A114), T137 (= T143), G138 (= G144), I178 (= I184), Y265 (≠ S273), G301 (= G309), D302 (= D310), M308 (≠ P316), L309 (≠ F317), A310 (≠ T318), H311 (= H319)

2yquA Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(e3s) from thermus thermophilus
30% identity, 98% coverage: 4:452/458 of query aligns to 2:443/455 of 2yquA

query
sites
2yquA

Y

Y

D

D

A

L

I

L

V

V

L

I

G
|
G

A

A

G
|
G

Q
x
P

S
x
G

G

G

M

Y

P

V

L

A

A

A

K

I

K

R

I

A

S

A

K

Q

L

L

G

G

L

M

S

K

V

V

A

G

L

V

I

V

E
|
E

K
|
K

-

E

R

K

V

A

I
x
L

G

G

G
|
G

T
|
T

C
|
C

I

L

N
x
R

D

V

G
|
G

C
|
C

S

I

P

P

T
x
S

K
|
K

T

A

M

L

V

L

-

E

S

T

A

E

R

R

V

I

A

Y

H

E

I

-

V

-

S

A

R

K

A

K

A

G

D

L

F

L

G

G

T

A

I

K

L

V

P

K

H
x
G

Y
x
V

R

E

I

L

D

D

Q

L

R

P

I

A

V

L

K

M

K

A

R

H

K

K

D

D

H

K

I

V

V

V

E

Q

L

A

F

N

R

T

G

Q

G

G

A

V

E

E

K

F

S

L

L

F

R

K

K

K

N

N

E

-

S

G

I

I

D

A

I

R

L

H

M

Q

G

G

S
x
T

A
|
A

A

R

F

F

K

L

D

S

S

E

R

R

T

K

I

V

Q

-

I

L

T

V

S

E

D

E

T

T

G

G

E

E

I

-

S

-

F

E

I

L

S

E

G

A

T

R

K

Y

I

I

F

L

I

I

N

A

T
|
T

G
|
G

S

S

E

A

P

P

R

L

I

I

P

P

E

P

I

W

E

A

G

Q

L

V

A

D

D

Y

T

E

P

R

Y

V

L

V

T

T

S
|
S

T

T

T

E

I

A

M

L

E

S

L

F

E

P

E

E

T

V

P

P

E

K

H

R

L

L

L

I

I

V

M

V

G

G

Y
x
V

I
|
I

G

G

L

L

E
|
E

F

L

A

G

Q

V

M

V

F

W

S

H

R

R

F

L

G

G

S

A

K

E

V

V

T

I

I

V

I

L

D

E

R

Y

A

M

E

D

R

R

L

I

V

L

H

P

K

T

E

M

D

D

E

L

D

E

V

V

C

S

K

R

E

A

I

A

S

E

Q

R

I

V

F

F

S

K

E

K

E

Q

G

G

I

L

D

T

T

I

L

R

F

T

N

G

A

V

E

R

V

V

R

T

K

A

V

V

S

V

Y

P

Q

E

-

A

D

K

G

G

F

A

K

R

L

V

T

E

T

L

E

E

T

-

P

-

K

-

G

G

L

G

L

E

D

V

I

L

K

E

G

A

S

D

H

R

L

V

L

L

V

V

A

A

T

V

G

G

R

R

I

R

P

P

S
x
Y

S

T

A

E

H

G

L

L

G

S

L

L

E

E

N

N

T

A

R

G

V

L

K

S

L

T

T

D

D

E

K

R

G

G

F

R

I

I

Q

P

T

V

D

D

D

E

F

H

L

L

Q

R

T

T

S

R

E

V

K

P

H

H

I

I

Y

Y

A

A

L

I

G
|
G

D
|
D

V

V

A

V

G

R

S

G

A

P

P
x
M

F
x
L

T
x
A

H

H

I

K

A

A

Y
x
S

H

E

D

E

A

G

H

I

I

A

A

A

F

V

Q

E

H

H

A

M

F

V

K

R

E

G

N

F

A

G

V

H

S

V

K

D

H

Y

E

Q

R

-

L

A

V

I

P

P

Y

S

C

V

V

V

F

Y

I

T

D

H

P

P

Q

E

L

I

G

A

R

A

I

V

G

G

L

Y

N

T

E

E

Q

E

E

E

A

L

Q

K

A

A

K

Q

G

G

I

I

P

P

Y

Y

K

K

T

V

G

G

K

K

F

F

L

P

M

Y

K

S

H

A

A

S

G

G

R

R

T

A

L

R

E

A

V

M

D

G

E

E

T

T

R

E

G

G

F

F

F

I

K

K

V

V

Q

L

V

A

D

H

P

A

E

K

S

T

K

D

K

R

I

I

L

L

G

G

A

V

T

H

I

G

L

I

S

G

L

A

D

R

G

V

G

G

E

D

I

V

L

L

A

A

T

E

L

A

Q

A

M

L

A

A

M

L

V

F

G

F

G

K

V

A

T

S

Y

A

D

E

T

D

I

L

A

G

T

R

L

A

P

P

I
x
H

A

A

H
|
H

P

P

T

S

L

L

A

S

E
|
E

S

I

L

L

N

K

N

E

active site: P11 (≠ Q13), L36 (≠ I37), C40 (= C41), C45 (= C46), S48 (≠ T49), G72 (≠ H74), V73 (≠ Y75), V177 (≠ Y183), E181 (= E187), S314 (≠ Y322), H432 (≠ I441), H434 (= H443), E439 (= E448)
binding flavin-adenine dinucleotide: G8 (= G10), G10 (= G12), P11 (≠ Q13), G12 (≠ S14), E31 (= E33), K32 (= K34), G38 (= G39), T39 (= T40), C40 (= C41), R42 (≠ N43), G44 (= G45), C45 (= C46), K49 (= K50), T110 (≠ S113), A111 (= A114), T137 (= T143), G138 (= G144), S157 (= S163), I178 (= I184), Y265 (≠ S273), G301 (= G309), D302 (= D310), M308 (≠ P316), L309 (≠ F317), A310 (≠ T318)

4k7zA Crystal structure of the c136(42)a/c141(47)a double mutant of tn501 mera in complex with NADP and hg2+
31% identity, 97% coverage: 8:450/458 of query aligns to 9:448/467 of 4k7zA

query
sites
4k7zA

V

V

L
x
I

G
|
G

A

S

G
|
G

Q
x
G

S
x
A

G

A

M

M

P

A

L

A

A

A

K

L

K

K

I

A

S

V

K

E

L

Q

G

G

L

A

S

Q

V

V

A

T

L

L

I

I

E
|
E

K
x
R

R

G

V

T

I
|
I

G

G

G
|
G

T
|
T

C
x
A

I

V

N

N

D

V

G
|
G

C
x
A

S

V

P

P

T
x
S

K
|
K

T

I

M

M

V

I

S

R

S

A

A

A

R

H

V

I

A

A

H

H

I

L

V

R

S

R

R

E

A

S

A

P

-

F

D

D

F

G

G

G

T

I

I

A

L

A

P

T

H
x
V

Y
x
P

R

T

I

I

D

D

Q

R

-

S

R

K

I

L

V

L

K

A

K

Q

R

Q

K

Q

D

A

H

R

I

V

V

D

E

E

L

L

F

R

R

H

G

A

G

K

A

Y

E

E

K

G

S

I

L

L

R

G

K

G

N

N

E

P

S

A

I

I

D

T

I

V

L

V

M

H

G

G

S
x
E

A
|
A

A

R

F

F

K

K

D

D

S

D

R

Q

T

S

I

L

Q

T

I

V

T

R

S

L

D

N

T

E

G

G

E

G

I

E

S

R

F

V

I

V

S

M

G

F

T

D

K

R

I

C

F

L

I

V

N

A

T
|
T

G
|
G

S

A

E

S

P

P

R

A

I

V

P

P

E

P

I

I

E

P

G

G

L

L

A

K

D

E

T

S

P

P

Y

Y

L

W

T

T

S

S

T

T

T

E

I

A

M

L

E

A

L

S

E

D

E

T

T

I

P

P

E

E

H

R

L

L

L

A

I

V

M

I

G

G

G
x
S

Y
x
V

I
x
V

G

A

L

L

E
|
E

F

L

A

A

Q

Q

M

A

F

F

S

A

R

R

F

L

G

G

S

S

K

K

V

V

T

T

I

V

I

L

D

A

R
|
R

A

-

E
x
N

R

T

L

L

V

F

H

F

K
x
R

E

E

D

D

E

P

D

A

V

I

C

G

K

E

E

A

I

V

S

T

Q

A

I

A

F

F

S

R

E

A

E

E

G

G

I

I

D

E

T

V

L

L

F

E

N

H

A

T

E

Q

V

A

R

S

K

Q

V

V

S

A

Y

H

Q

M

D

D

G

G

-

E

F

F

K

V

L

L

T

T

E

T

T

H

P

G

K

-

G

-

L

-

D

E

I

L

K

R

G

A

S

D

H

K

L

L

V

V

A

A

T
|
T

G
|
G

R
|
R

I

T

P

P

S

N

S

T

A

R

H

S

L

L

G

A

L

L

E

D

N

A

T

A

R

G

V

V

K

T

L

V

T

N

D

A

K

Q

G

G

F

A

I

I

Q

V

T

I

D

D

D

Q

F

G

L

M

Q

R

T

T

S

S

E

N

K

P

H

N

I

I

Y

Y

A

A

L

A

G
|
G

D
|
D

V

C

A

T

G

D

S

Q

A

P

P
x
Q

F
|
F

T
x
V

H
x
Y

I

V

A

A

Y
x
A

H

A

D

A

A

G

H

T

I

R

A

A

F

A

Q

I

H

N

A

M

F

T

K

G

E

G

N

D

A

A

V

A

S

L

K

D

H

L

E

T

R

A

L

M

V

-

P

P

Y

A

C
x
V

V

V

F

F

I

T

D

D

P

P

Q

Q

L

V

G

A

R

T

I

V

G

G

L

Y

N

S

E

E

Q

A

E

E

A

A

Q

H

A

H

K

D

G

G

I

I

P

E

Y

T

K

D

T

S

G

R

K

T

F

L

L

T

M

L

K

D

H

N

A

V

G

P

R

R

T

A

L

L

E

A

V

N

D

F

E

D

T

T

R

R

G

G

F

F

F

I

K

K

V

L

Q

V

V

I

D

E

P

E

E

G

S

S

K

H

K

R

I

L

L

I

G

G

A

V

T

Q

I

A

L

V

S

A

L

P

D

E

G

A

G

G

E

E

I

L

L

I

A

Q

T

T

L

A

Q

A

M

L

A

A

M

I

V

R

G

N

G

R

V

M

T

T

Y

V

D

Q

T

E

I

L

A

A

T

D

L

Q

P

L

I
x
F

A

P

H
x
Y

P

L

T

T

L

M

A

V

E
|
E

S

G

L

L

active site: G14 (≠ Q13), I38 (= I37), A42 (≠ C41), A47 (≠ C46), S50 (≠ T49), V76 (≠ H74), P77 (≠ Y75), V186 (≠ Y183), E190 (= E187), A321 (≠ Y322), F439 (≠ I441), Y441 (≠ H443), E446 (= E448)
binding flavin-adenine dinucleotide: I10 (≠ L9), G11 (= G10), G13 (= G12), A15 (≠ S14), E34 (= E33), R35 (≠ K34), G40 (= G39), T41 (= T40), A42 (≠ C41), G46 (= G45), A47 (≠ C46), K51 (= K50), E116 (≠ S113), A117 (= A114), T146 (= T143), G147 (= G144), R269 (= R270), G308 (= G309), D309 (= D310), Q315 (≠ P316), F316 (= F317), V317 (≠ T318), Y318 (≠ H319)
binding nadp nicotinamide-adenine-dinucleotide phosphate: S184 (≠ G181), S185 (≠ G182), V186 (≠ Y183), V187 (≠ I184), E190 (= E187), R207 (= R204), N208 (≠ E206), R213 (≠ K211), T267 (= T268), G268 (= G269), R269 (= R270), Q315 (≠ P316), F316 (= F317), V346 (≠ C348)

Sites not aligning to the query:

active site: 464, 465

Query Sequence

>Echvi_0722 Echvi_0722 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes
MENYDAIVLGAGQSGMPLAKKISKLGLSVALIEKRVIGGTCINDGCSPTKTMVSSARVAH
IVSRAADFGTILPHYRIDQRIVKKRKDHIVELFRGGAEKSLRKNESIDILMGSAAFKDSR
TIQITSDTGEISFISGTKIFINTGSEPRIPEIEGLADTPYLTSTTIMELEETPEHLLIMG
GGYIGLEFAQMFSRFGSKVTIIDRAERLVHKEDEDVCKEISQIFSEEGIDTLFNAEVRKV
SYQDGFKLTTETPKGLLDIKGSHLLVATGRIPSSAHLGLENTRVKLTDKGFIQTDDFLQT
SEKHIYALGDVAGSAPFTHIAYHDAHIAFQHAFKENAVSKHERLVPYCVFIDPQLGRIGL
NEQEAQAKGIPYKTGKFLMKHAGRTLEVDETRGFFKVQVDPESKKILGATILSLDGGEIL
ATLQMAMVGGVTYDTIATLPIAHPTLAESLNNLMGQIE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory