SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_0731 FitnessBrowser__Cola:Echvi_0731 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

5odqB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with bromoethanesulfonate. (see paper)
24% identity, 92% coverage: 23:264/264 of query aligns to 5:271/291 of 5odqB

query
sites
5odqB

F

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G26), C9 (= C27), C41 (≠ T61), C42 (= C62), C78 (= C99), G80 (≠ H101), C81 (= C102), G152 (≠ S153), C153 (= C154), H154 (≠ Y155), C193 (= C189), C194 (= C190), C231 (= C228), F233 (= F230), C234 (= C231)
binding 2-bromanylethanesulfonic acid: G46 (≠ P66), G80 (≠ H101), H154 (≠ Y155), G197 (= G193), R201 (≠ M197), F233 (= F230), L236 (≠ T233)

5odhH Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
24% identity, 92% coverage: 23:264/264 of query aligns to 5:271/291 of 5odhH

query
sites
5odhH

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G26), C9 (= C27), C41 (≠ T61), C42 (= C62), C78 (= C99), G80 (≠ H101), C81 (= C102), C153 (= C154), H154 (≠ Y155), C193 (= C189), C194 (= C190), C231 (= C228), F233 (= F230), C234 (= C231)
binding 1-thioethanesulfonic acid: A44 (≠ G64), P45 (≠ D65), G46 (≠ P66), G80 (≠ H101), H154 (≠ Y155), G197 (= G193), G198 (= G194)
binding Coenzyme B: R201 (≠ M197), F233 (= F230), R240 (≠ D237)

5odhB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
24% identity, 92% coverage: 23:264/264 of query aligns to 5:271/291 of 5odhB

query
sites
5odhB

F

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G26), C9 (= C27), I10 (≠ A28), C41 (≠ T61), C42 (= C62), C78 (= C99), G80 (≠ H101), C81 (= C102), G152 (≠ S153), C153 (= C154), H154 (≠ Y155), C193 (= C189), C194 (= C190), C231 (= C228), F233 (= F230), C234 (= C231)
binding 1-thioethanesulfonic acid: P45 (≠ D65), G46 (≠ P66), G80 (≠ H101), F233 (= F230)

5odcB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
24% identity, 92% coverage: 23:264/264 of query aligns to 5:271/291 of 5odcB

query
sites
5odcB

F

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G26), C9 (= C27), I10 (≠ A28), C41 (≠ T61), C42 (= C62), C78 (= C99), N79 (≠ P100), G80 (≠ H101), C81 (= C102), G152 (≠ S153), C153 (= C154), C193 (= C189), C194 (= C190), G197 (= G193), C231 (= C228), F233 (= F230), C234 (= C231)

Query Sequence

>Echvi_0731 FitnessBrowser__Cola:Echvi_0731
MSNYKVPTMAEMAASGESPEILFWVGCAGSFDDRYKAVTQAFVKILNKVGVSFAVLGPEE
TCTGDPARRAGNEFLFQMQAVANIQVMNGYNVKKVVTACPHCFNTIKNEYPALGGEYEVI
HHSQFLQSLINDGKIVMKGGGEFKGKKITFHDSCYLGRANNVYEAPREIIKALDVELVEM
KRCRTKGLCCGAGGAQMFKEPEPGNKDINVERTEEALGTGASAIAVGCPFCLTMMADGVK
NKEKESDVKVVDLAELIAKDQGLD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory