SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_0942 FitnessBrowser__Cola:Echvi_0942 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
35% identity, 53% coverage: 102:232/246 of query aligns to 57:183/185 of 3nz2J

query
sites
3nz2J

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binding acetyl coenzyme *a: G104 (≠ S151), Y110 (= Y157), H114 (= H161), W138 (= W187), G140 (≠ A189), A158 (= A207), A159 (= A208), N164 (≠ T213), G174 (= G223), T176 (≠ N225), P177 (= P226), R182 (≠ K231)

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
35% identity, 53% coverage: 102:232/246 of query aligns to 47:173/176 of 3ectA

query
sites
3ectA

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binding calcium ion: G138 (= G197), D156 (= D215)

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
35% identity, 53% coverage: 102:232/246 of query aligns to 54:180/183 of 3nz2C

query
sites
3nz2C

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binding acetyl coenzyme *a: Y107 (= Y157), H111 (= H161), G137 (≠ A189), A155 (= A207), A156 (= A208), N161 (≠ T213), G171 (= G223), T173 (≠ N225), P174 (= P226), L178 (= L230), R179 (≠ K231)
binding magnesium ion: N157 (≠ G209), T173 (≠ N225)

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
47% identity, 37% coverage: 142:231/246 of query aligns to 95:182/186 of 4isxA

query
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4isxA

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binding acetyl coenzyme *a: A104 (≠ S151), W138 (= W187), G140 (≠ A189), G141 (≠ S190), A159 (= A208), V174 (≠ G223), K182 (= K231)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
36% identity, 48% coverage: 114:231/246 of query aligns to 67:182/190 of 5u2kA

query
sites
5u2kA

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binding coenzyme a: M90 (≠ G137), Y110 (= Y157), A112 (≠ E159), H114 (= H161), L146 (= L195), T164 (= T213), K165 (= K214)

3igjC Crystal structure of maltose o-acetyltransferase complexed with acetyl coenzyme a from bacillus anthracis
39% identity, 37% coverage: 142:232/246 of query aligns to 97:185/188 of 3igjC

query
sites
3igjC

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binding acetyl coenzyme *a: A106 (≠ S151), Y112 (= Y157), A114 (≠ E159), H116 (= H161), G142 (≠ A189), N148 (≠ L195), A160 (= A207), S161 (≠ A208), T166 (= T213), N178 (= N225)

Sites not aligning to the query:

binding acetyl coenzyme *a: 84

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
37% identity, 48% coverage: 122:238/246 of query aligns to 61:172/204 of 1mrlA

query
sites
1mrlA

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binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: N81 (= N147), H82 (≠ V148), L93 (≠ E159), M102 (≠ S168), L108 (≠ G174)

Sites not aligning to the query:

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: 37, 39, 54, 56

6u9cA The 2.2 a crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with acetyl coa
44% identity, 32% coverage: 156:234/246 of query aligns to 89:163/206 of 6u9cA

query
sites
6u9cA

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binding acetyl coenzyme *a: W116 (= W187), A136 (= A207), I159 (≠ L230)

3dhoA Structure of streptogramin acetyltransferase in complex with an inhibitor
37% identity, 48% coverage: 122:238/246 of query aligns to 61:172/203 of 3dhoA

query
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3dhoA

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binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: M84 (= M150), I139 (≠ V205), A141 (= A207), V147 (≠ T213), L155 (≠ I221), G157 (= G223), P160 (= P226), I164 (≠ L230)

6pubA Crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with crystal violet
44% identity, 32% coverage: 156:234/246 of query aligns to 92:166/210 of 6pubA

query
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6pubA

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F

Sites not aligning to the query:

binding crystal violet: 4, 7, 9, 10, 11, 36, 37

P50870 Streptogramin A acetyltransferase; Virginiamycin acetyltransferase D; Vat(D); EC 2.3.1.- from Enterococcus faecium (Streptococcus faecium) (see paper)
37% identity, 48% coverage: 122:238/246 of query aligns to 61:172/209 of P50870

query
sites
P50870

L

L

K

K

V

I

G

G

N

K

N

F

S

C

S

S

I

I

G

G

P

P

Y

G

A

V

Y

T

I

I

G

-

C

-

S

-

G

-

Y

-

I

I

E

M

I

N

G

G

D

A

N

N

V
x
H

M

R

M

M

S

D

P

G

R

S

V

T

S

Y

I

P

Y

F

S

N

E

L

N

F

H

G

N

N

F

G

D

W

D

E

T

K

E

H

S

M

P

P

M

K

I

L

E

D

Q

Q

G

L

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P

T

I

R

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G

F

D

A

T

K

I

I

I

E

G

D

N

D

D

C

V

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W

I

I

A

G

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K

N

D

S

V

I

V

I

I

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A

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V

T

K

V

I

G

G

K

D

G

G

S

A

V

I

V

V

A

A

G

N

S

S

V

V

T

V

K

K

D

D

V

I

P

A

P

P

Y

Y

S

M

I

L

V

A

G

G

N

N

P

P

A

A

K

N

L

E

L

I

K

K

T

Q

R

R

F

F

E

D

Q

Q

K

D

S

T

H82 (≠ V148) mutation to A: 105-fold decrease in activity.

1khrA Crystal structure of vat(d) in complex with virginiamycin and coenzyme a (see paper)
37% identity, 48% coverage: 122:238/246 of query aligns to 61:172/206 of 1khrA

query
sites
1khrA

L

L

K

K

V

I

G

G

N

K

N

F

S

C

S

S

I

I

G

G

P

P

Y

G

A

V

Y

T

I

I

G

-

C

-

S

-

G

-

Y

-

I

I

E

M

I

N

G

G

D
x
A

N
|
N

V
x
H

M

R

M
|
M

S

D

P

G

R

S

V

T

S

Y

I

P

Y

F

S

N

E
x
L

N

F

H

G

N

N

F

G

D

W

D

E

T

K

E

H

S

M

P

P

M

K

I

L

E

D

Q

Q

G

L

V

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T

I

R

K

S

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F

D

A

T

K

I

I

I

E

G

D

N

D

D

C

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W
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W

I

I

A

G

S

K

N

D

S

V

I

V

I

I

L

M

A

P

G

G

V

V

T

K

V

I

G

G

K

D

G

G

S

A

V

I

V

V

A
|
A

G

N

S

S

V

V

T
x
V

K

K

D

D

V

I

P

A

P

P

Y

Y

S

M

I
x
L

V

A

G
|
G

G

G

N

N

P
|
P

A

A

K

N

L

E

L
x
I

K

K

T

Q

R

R

F

F

E

D

Q

Q

K

D

S

T

binding coenzyme a: H82 (≠ V148), M84 (= M150), W121 (= W187), A141 (= A207), A142 (= A208), V147 (≠ T213), L155 (≠ I221), G157 (= G223), P160 (= P226), I164 (≠ L230)
binding virginiamycin m1: A80 (≠ D146), N81 (= N147), H82 (≠ V148), L93 (≠ E159)

Sites not aligning to the query:

binding virginiamycin m1: 37, 39, 54

1kk4A Crystal structure of vat(d) in complex with acetyl-coa (see paper)
37% identity, 48% coverage: 122:238/246 of query aligns to 61:172/205 of 1kk4A

query
sites
1kk4A

L

L

K

K

V

I

G

G

N

K

N

F

S

C

S

S

I
|
I

G
|
G

P

P

Y

G

A

V

Y

T

I

I

G

-

C

-

S

-

G

-

Y

-

I

I

E

M

I

N

G

G

D

A

N

N

V

H

M

R

M

M

S

D

P

G

R

S

V

T

S

Y

I

P

Y

F

S

N

E

L

N

F

H

G

N

N

F

G

D

W

D

E

T

K

E

H

S

M

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P

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K

I

L

E

D

Q

Q

G

L

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T

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x
K

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D

A

T

K

I

I

I

E

G

D

N

D

D

C

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W
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W

I

I

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x
G

S
x
K

N

D

S

V

I

V

I

I

L

M

A

P

G

G

V

V

T

K

V

I

G

G

K

D

G

G

S

A

V

I

V

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A
|
A

G

N

S

S

V

V

T
x
V

K
|
K

D

D

V

I

P

A

P

P

Y

Y

S

M

I
x
L

V

A

G
|
G

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N

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|
P

A

A

K

N

L

E

L
x
I

K

K

T

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R

R

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E

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Q

Q

K

D

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T

binding acetyl coenzyme *a: I69 (= I130), G70 (= G131), K111 (≠ R177), W121 (= W187), G123 (≠ A189), K124 (≠ S190), A141 (= A207), A142 (= A208), V147 (≠ T213), K148 (= K214), L155 (≠ I221), G157 (= G223), G158 (= G224), P160 (= P226), I164 (≠ L230)

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
52% identity, 22% coverage: 181:234/246 of query aligns to 114:167/211 of 4hurA

query
sites
4hurA

K

E

I

I

E

G

D

N

D

D

C

V

W
|
W

I
|
I

A
x
G

S
x
R

N

D

S

V

I

T

I

I

L
x
M

A

P

G

G

V

V

T

K

V

I

G

G

K

D

G

G

S

A

V

I

A
|
A

G

E

S

A

V

V

T
|
T

K

K

D

N

V

V

P

A

P

P

Y

Y

S

S

I

I

V

V

G
|
G

G

G

N

N

P
|
P

A

L

K

K

L

F

L
x
I

K
x
R

T

K

R

R

F

F

binding acetyl coenzyme *a: W120 (= W187), I121 (= I188), G122 (≠ A189), R123 (≠ S190), M128 (≠ L195), A140 (= A207), A141 (= A208), T146 (= T213), G156 (= G223), P159 (= P226), I163 (≠ L230), R164 (≠ K231)

Sites not aligning to the query:

binding acetyl coenzyme *a: 51, 67, 68, 69, 79, 80, 110

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
52% identity, 22% coverage: 181:234/246 of query aligns to 114:167/212 of 4husA

query
sites
4husA

K

E

I

I

E

G

D

N

D

D

C

V

W

W

I

I

A

G

S

R

N

D

S

V

I

T

I

I

L

M

A

P

G

G

V

V

T

K

V

I

G

G

K

D

G

G

S

A

V

I

A

A

G

E

S

A

V

V

T

T

K

K

D

N

V

V

P

A

P

P

Y

Y

S

S

I

I

V

V

G

G

N

N

P

P

A

L

K

K

L

F

L

I

K

R

T

K

R

R

F

F

Sites not aligning to the query:

binding virginiamycin m1: 17, 36, 38

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
52% identity, 22% coverage: 181:234/246 of query aligns to 114:167/207 of 6x3cA

query
sites
6x3cA

K

E

I

I

E

G

D

N

D

D

C

V

W
|
W

I

I

A
x
G

S
x
R

N

D

S

V

I

T

I

I

L
x
M

A

P

G

G

V

V

T

K

V

I

G

G

K

D

G

G

S

A

V

I

A
|
A

G

E

S

A

V

V

T

T

K

K

D

N

V

V

P

A

P

P

Y

Y

S

S

I

I

V

V

G

G

N

N

P

P

A

L

K

K

L

F

L

I

K

R

T

K

R

R

F

F

binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: W120 (= W187), G122 (≠ A189), R123 (≠ S190), M128 (≠ L195), A140 (= A207), A141 (= A208)

Sites not aligning to the query:

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
52% identity, 22% coverage: 181:234/246 of query aligns to 114:167/206 of 6x3jA

query
sites
6x3jA

K

E

I

I

E

G

D

N

D

D

C

V

W
|
W

I

I

A
x
G

S

R

N

D

S

V

I

T

I

I

L

M

A

P

G

G

V

V

T

K

V

I

G

G

K

D

G

G

S

A

V

I

A

A

G

E

S

A

V

V

T

T

K

K

D

N

V

V

P

A

P

P

Y

Y

S

S

I

I

V

V

G

G

N

N

P

P

A

L

K

K

L

F

L

I

K

R

T

K

R

R

F

F

binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: W120 (= W187), G122 (≠ A189)

Sites not aligning to the query:

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
52% identity, 22% coverage: 181:234/246 of query aligns to 114:167/203 of 6x3cE

query
sites
6x3cE

K

E

I

I

E

G

D

N

D

D

C

V

W
|
W

I

I

A
x
G

S

R

N

D

S

V

I

T

I

I

L

M

A

P

G

G

V

V

T

K

V

I

G

G

K

D

G

G

S

A

V

I

A
|
A

G

E

S

A

V

V

T

T

K

K

D

N

V

V

P

A

P

P

Y

Y

S

S

I

I

V

V

G

G

N
|
N

P
|
P

A

L

K

K

L

F

L

I

K

R

T

K

R

R

F

F

binding magnesium ion: N158 (= N225), P159 (= P226)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: W120 (= W187), G122 (≠ A189), A140 (= A207), A141 (= A208)

Sites not aligning to the query:

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
32% identity, 53% coverage: 101:231/246 of query aligns to 56:182/200 of 1krrA

query
sites
1krrA

T

T

V

V

G

G

S

E

Y

N

A

A

I

W

I

V

R

E

P

P

T

P

N

V

L

Y

Y

F

G

S

G

-

E

-

A

Y

G

G

V

S

G

N

L

I

K

H

V

I

G

G

N

R

N

N

S

F

S

Y

I

A

G
x
N

P

F

Y

N

A

L

Y

T

I

I

G

V

C

D

S

D

G

Y

Y

T

I

V

E

T

I

I

G

G

D

D

N

N

V

V

M

L

M
x
I

S
x
A

P
|
P

R

N

V

V

S

T

I

L

Y

S

S

V

E
x
T

N

G

H
|
H

N

-

F

-

D

-

D

P

T

V

E

H

S

H

P

E

M

L

I

R

E

K

Q

N

G

G

V

E

T

M

R

Y

S

S

F

F

A

P

-

I

K

T

I

I

E

G

D

N

C

V

W

W

I

I

A
x
G

S
|
S

N

H

S

V

I

V

I

I

L
x
N

A

P

G

G

V

V

T

T

V

I

G

G

K

D

G

N

S

S

V

V

V

I

A
x
G

A
|
A

G

G

S

S

V

I

V

V

T

T

K

K

D

D

V

I

P

P

Y

N

S

V

I

V

V

A

G
x
A

G

G

N

V

P
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P

A

C

K

R

L

V

L

I

K
x
R

binding acetyl coenzyme *a: N84 (≠ G131), I103 (≠ M150), A104 (≠ S151), P105 (= P152), T112 (≠ E159), H114 (= H161), G140 (≠ A189), S141 (= S190), N146 (≠ L195), G158 (≠ A207), A159 (= A208), A174 (≠ G223), P177 (= P226), R182 (≠ K231)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
32% identity, 53% coverage: 101:231/246 of query aligns to 57:183/203 of P07464

query
sites
P07464

T

T

V

V

G

G

S

E

Y

N

A

A

I

W

I

V

R

E

P

P

T

P

N

V

L

Y

Y

F

G
x
S

G

-

E

-

A

Y

G

G

V

S

G

N

L

I

K

H

V

I

G

G

N

R

N

N

S

F

S

Y

I

A

G
x
N

P

F

Y

N

A

L

Y

T

I

I

G

V

C

D

S
x
D

G

Y

Y

T

I

V

E

T

I

I

G

G

D

D

N

N

V

V

M

L

M

I

S

A

P

P

R

N

V

V

S

T

I

L

Y

S

S

V

E

T

N

G

H
|
H

N

-

F

-

D

-

D

P

T

V

E

H

S

H

P

E

M

L

I

R

E

K

Q

N

G

G

V

E

T

M

R

Y

S

S

F

F

A

P

-

I

K

T

I

I

E

G

D

N

C

V

W

W

I

I

A

G

S
|
S

N

H

S

V

I

V

I

I

L

N

A

P

G

G

V

V

T

T

V

I

G

G

K

D

G

N

S

S

V

V

V

I

A

G

A
|
A

G

G

S

S

V

I

V

V

T
|
T

K
|
K

D

D

V

I

P

P

Y

N

S

V

I

V

V

A

G

A

G

G

N

V

P

P

A

C

K
x
R

L

V

L

I

K
x
R

S71 (≠ G115) binding
N85 (≠ G131) binding in other chain; binding in other chain
D93 (≠ S139) binding
H115 (= H161) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (= S190) binding in other chain
A160 (= A208) binding in other chain
TK 165:166 (= TK 213:214) binding
R180 (≠ K228) binding
R183 (≠ K231) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding

Query Sequence

>Echvi_0942 FitnessBrowser__Cola:Echvi_0942
MANFIQRTLTNLTGIDLSDRFDENWDSLSTLGVLWRMSGWFIRGCFRRLWFKSSEGMLLI
GKRVTIRQARYLSVGKNFIAQDNCEINCLSQKGIVFGDKVTVGSYAIIRPTNLYGGEAGV
GLKVGNNSSIGPYAYIGCSGYIEIGDNVMMSPRVSIYSENHNFDDTESPMIEQGVTRSFA
KIEDDCWIASNSIILAGVTVGKGSVVAAGSVVTKDVPPYSIVGGNPAKLLKTRFEQKSAG
KTANDQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory