SitesBLAST
Comparing Echvi_1096 Echvi_1096 nitrate transport ATP-binding subunits C and D to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
40% identity, 66% coverage: 3:196/294 of query aligns to 3:202/226 of 5xu1B
P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
35% identity, 79% coverage: 1:232/294 of query aligns to 1:229/393 of P9WQI3
- H193 (≠ N195) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
7ahhC Opua inhibited inward-facing, sbd docked (see paper)
36% identity, 66% coverage: 25:219/294 of query aligns to 44:245/382 of 7ahhC
Sites not aligning to the query:
- binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298
- binding phosphoaminophosphonic acid-adenylate ester: 12, 39, 40, 41
7aheC Opua inhibited inward facing (see paper)
36% identity, 66% coverage: 25:219/294 of query aligns to 44:245/382 of 7aheC
Sites not aligning to the query:
- binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298
7ahdC Opua (e190q) occluded (see paper)
36% identity, 66% coverage: 25:219/294 of query aligns to 44:245/260 of 7ahdC
- binding adenosine-5'-triphosphate: S61 (= S42), G62 (= G43), G64 (= G45), K65 (= K46), S66 (≠ T47), T67 (= T48), Q111 (= Q83), K161 (= K135), Q162 (≠ E136), S164 (= S138), G166 (= G140), M167 (= M141), Q188 (≠ E162), H221 (≠ N195)
Sites not aligning to the query:
2d62A Crystal structure of multiple sugar binding transport atp- binding protein
33% identity, 74% coverage: 1:219/294 of query aligns to 4:225/375 of 2d62A
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
37% identity, 74% coverage: 1:219/294 of query aligns to 1:216/371 of P68187
- A85 (≠ S86) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ E112) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ I117) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ Y120) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ G122) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ T127) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G140) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D161) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
Sites not aligning to the query:
- 228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
37% identity, 74% coverage: 2:219/294 of query aligns to 1:215/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F13), S37 (= S42), G38 (= G43), C39 (≠ S44), G40 (= G45), K41 (= K46), S42 (≠ T47), T43 (= T48), Q81 (= Q83), R128 (≠ K132), A132 (≠ E136), S134 (= S138), G136 (= G140), Q137 (≠ M141), E158 (= E162), H191 (≠ N195)
- binding magnesium ion: S42 (≠ T47), Q81 (= Q83)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
37% identity, 74% coverage: 2:219/294 of query aligns to 1:215/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F13), G38 (= G43), C39 (≠ S44), G40 (= G45), K41 (= K46), S42 (≠ T47), T43 (= T48), R128 (≠ K132), S134 (= S138), Q137 (≠ M141)
- binding beryllium trifluoride ion: S37 (= S42), G38 (= G43), K41 (= K46), Q81 (= Q83), S134 (= S138), G136 (= G140), H191 (≠ N195)
- binding magnesium ion: S42 (≠ T47), Q81 (= Q83)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
37% identity, 74% coverage: 2:219/294 of query aligns to 1:215/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F13), V17 (= V22), G38 (= G43), C39 (≠ S44), G40 (= G45), K41 (= K46), S42 (≠ T47), T43 (= T48), R128 (≠ K132), A132 (≠ E136), S134 (= S138), Q137 (≠ M141)
- binding tetrafluoroaluminate ion: S37 (= S42), G38 (= G43), K41 (= K46), Q81 (= Q83), S134 (= S138), G135 (= G139), G136 (= G140), E158 (= E162), H191 (≠ N195)
- binding magnesium ion: S42 (≠ T47), Q81 (= Q83)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
37% identity, 74% coverage: 2:219/294 of query aligns to 1:215/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ F13), V17 (= V22), G38 (= G43), C39 (≠ S44), G40 (= G45), K41 (= K46), S42 (≠ T47), T43 (= T48), R128 (≠ K132), A132 (≠ E136), S134 (= S138), Q137 (≠ M141)
- binding magnesium ion: S42 (≠ T47), Q81 (= Q83)
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
37% identity, 74% coverage: 2:219/294 of query aligns to 1:215/374 of 2awnB
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
37% identity, 73% coverage: 4:219/294 of query aligns to 1:213/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ F13), S35 (= S42), G36 (= G43), C37 (≠ S44), G38 (= G45), K39 (= K46), S40 (≠ T47), T41 (= T48), R126 (≠ K132), A130 (≠ E136), S132 (= S138), G134 (= G140), Q135 (≠ M141)
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
36% identity, 74% coverage: 1:219/294 of query aligns to 1:216/369 of P19566
- L86 (= L87) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P163) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D168) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
1g291 Malk (see paper)
33% identity, 74% coverage: 1:219/294 of query aligns to 1:222/372 of 1g291
- binding magnesium ion: D69 (≠ G73), E71 (≠ G75), K72 (≠ P76), K79 (vs. gap), D80 (vs. gap)
- binding pyrophosphate 2-: S38 (= S42), G39 (= G43), C40 (≠ S44), G41 (= G45), K42 (= K46), T43 (= T47), T44 (= T48)
Sites not aligning to the query:
1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
35% identity, 72% coverage: 3:213/294 of query aligns to 1:221/232 of 1f3oA
1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
34% identity, 72% coverage: 3:213/294 of query aligns to 1:221/230 of 1l2tA
- binding adenosine-5'-triphosphate: Y11 (≠ F13), S40 (= S42), G41 (= G43), S42 (= S44), G43 (= G45), K44 (= K46), S45 (≠ T47), T46 (= T48), F138 (≠ M130), Q145 (≠ E136), S147 (= S138), G149 (= G140), Q150 (≠ M141), H204 (≠ N195)
8hplC Lpqy-sugabc in state 1 (see paper)
32% identity, 74% coverage: 2:219/294 of query aligns to 1:214/384 of 8hplC
8hprD Lpqy-sugabc in state 4 (see paper)
32% identity, 74% coverage: 2:219/294 of query aligns to 1:216/362 of 8hprD
- binding adenosine-5'-triphosphate: Y12 (≠ F13), S38 (= S42), C40 (≠ S44), G41 (= G45), K42 (= K46), S43 (≠ T47), T44 (= T48), Q82 (= Q83), R129 (≠ K132), Q133 (≠ E136), S135 (= S138), G136 (= G139), G137 (= G140), Q159 (≠ E162), H192 (≠ N195)
- binding magnesium ion: S43 (≠ T47), Q82 (= Q83)
8hprC Lpqy-sugabc in state 4 (see paper)
32% identity, 74% coverage: 2:219/294 of query aligns to 1:216/363 of 8hprC
- binding adenosine-5'-triphosphate: Y12 (≠ F13), S38 (= S42), G39 (= G43), G41 (= G45), K42 (= K46), S43 (≠ T47), Q82 (= Q83), Q133 (≠ E136), G136 (= G139), G137 (= G140), Q138 (≠ M141), H192 (≠ N195)
- binding magnesium ion: S43 (≠ T47), Q82 (= Q83)
Query Sequence
>Echvi_1096 Echvi_1096 nitrate transport ATP-binding subunits C and D
MAIIELNNVSKSFGMGASRVDVLSDINLQVAEGEFVAIVGFSGSGKTTLINLLNGLAFPD
QGEVLLHGQPVTGPGPDRGVIFQNYSLLPWLSVYNNVKLAVDEVFPQLSSKEKASHIKKY
IGMVNLTPAMDKLPKELSGGMRQRVSVARALAMNPEILLMDEPLSALDALTRGSLQEEII
RIWSQDKKTAILITNDVDEGILMADRIIPLTPGPKATLGPEFTIDIERPRDLSAINQNDD
YKKLRNEIIEYLIEVGASRKKSGDQHYILPDLKPVLPGRVFPGIKRKSEKVKYF
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory