SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_1188 FitnessBrowser__Cola:Echvi_1188 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6ymdA Crystal structure of serine hydroxymethyltransferase from aphanothece halophytica in the covalent complex with malonate (see paper)
57% identity, 100% coverage: 1:422/422 of query aligns to 8:414/420 of 6ymdA

query
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6ymdA

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active site: Y54 (= Y47), E56 (= E49), D200 (= D193), T226 (= T219), K229 (= K222), R235 (= R228)
binding malonate ion: S34 (= S27), Y54 (= Y47), E56 (= E49), Y64 (= Y57), H125 (= H118), H203 (= H196), K229 (= K222), R361 (= R369)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: Y54 (= Y47), S96 (= S89), G97 (= G90), A98 (= A91), H125 (= H118), Y174 (≠ A167), S175 (= S168), D200 (= D193), A202 (≠ S195), T226 (= T219), K229 (= K222), G261 (= G269)

6ymfA Crystal structure of serine hydroxymethyltransferase from aphanothece halophytica in the plp-serine external aldimine state (see paper)
57% identity, 100% coverage: 1:422/422 of query aligns to 8:414/418 of 6ymfA

query
sites
6ymfA

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active site: Y54 (= Y47), E56 (= E49), D200 (= D193), T226 (= T219), K229 (= K222), R235 (= R228)
binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: S34 (= S27), S96 (= S89), G97 (= G90), A98 (= A91), H125 (= H118), S175 (= S168), D200 (= D193), A202 (≠ S195), H203 (= H196), T226 (= T219), K229 (= K222), R361 (= R369)

Q5SI56 Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
56% identity, 100% coverage: 1:421/422 of query aligns to 5:406/407 of Q5SI56

query
sites
Q5SI56

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M

T

P

K

L

I

V

V

A

D

E

L

L

I

I

D

D

R

R

A

A

L

L

M

L

N

E

H

G

E

P

D

S

E

E

A

A

A

-

L

-

A

-

K

-

I

L

K

R

Q

E

E

E

V

V

N

R

D

R

W

L

M

A

V

L

Q

A

F

H

P

P

L

M

Y51 (= Y47) binding pyridoxal 5'-phosphate
GS 94:95 (≠ GA 90:91) binding pyridoxal 5'-phosphate
S172 (= S168) binding pyridoxal 5'-phosphate
H200 (= H196) binding pyridoxal 5'-phosphate
H225 (= H221) binding pyridoxal 5'-phosphate
K226 (= K222) modified: N6-(pyridoxal phosphate)lysine
G258 (= G269) binding pyridoxal 5'-phosphate

8suiB Joint x-ray/neutron structure of thermus thermophilus serine hydroxymethyltransferase (tthshmt) in internal aldimine state with l- ser bound in a pre-michalis complex (see paper)
56% identity, 100% coverage: 2:421/422 of query aligns to 1:401/402 of 8suiB

query
sites
8suiB

K

K

R

R

D

D

Q

E

V

A

I

L

F

F

D

E

L

L

I

I

Q

A

K

L

E

E

E

E

D

K

R

R

Q

Q

K

R

R

E

G

G

I

L

E

E

L

L

I

I

A

A

S

S

E

E

N

N

F

F

T

V

S

S

K

K

Q

Q

V

V

M

R

E

E

A

A

A

V

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y

Y

A

A

E

E

G

G

L

Y

P

P

K

G

K

A

R

R

Y
|
Y

Y
|
Y

G

G

G

G

C

C

E

E

V

V

V

I

D

D

D

R

I

V

E

E

Q

S

I

L

A

A

I

I

D

E

R

R

A

A

K

K

E

A

L

L

F

F

G

G

A

A

T

A

W

W

A

A

N

N

V

V

Q

Q

P

P

H

H

S

S

G

G

A

S

Q

Q

A

A

N

N

A

M

A

A

V

V

F

Y

L

M

A

A

C

L

L

M

N

E

P

P

G

G

D

D

H

T

I

L

L

M

G

G

F

M

D

D

L

L

S

A

H

A

G

G

G

G

H

H

L

L

T

T

H

H

G

G

S

S

P

R

V

V

N

N

F

F

S

S

G

G

K

K

N

L

Y

Y

K

K

P

V

H

V

F

S

Y

Y

G

G

V

V

E

R

E

P

E

D

T

T

G

E

I

L

I

I

D

D

M

L

D

E

K

E

V

V

A

R

E

R

K

L

A

A

R

L

E

E

V

H

K

R

P

P

K

K

L

V

I

I

I

V

C

A

G

G

A

A

S

S

A

A

Y

Y

S

P

R

R

D

F

W

W

D

D

Y

F

A

K

R

A

F

F

R

R

E

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

L

Y

L

L

L

V

A

V

D

D

I

M

S

A

H

H

P

F

A

A

G

G

L

L

I

V

A

A

R

A

G

G

L

L

L

H

K

P

D

N

P

P

L

L

E

P

H

Y

C

A

H

H

I

V

V

V

T

T

T

S

T

T

T

T

H

H

K

K

T

T

L

L

R

R

G

G

T

P

R

R

G

G

G

G

L

L

I

I

M

L

M

S

R

-

E

-

D

-

F

-

D

N

N

D

P

P

F

-

G

-

I

-

K

-

N

-

P

-

K

-

G

-

E

-

L

-

R

-

K

E

M

L

T

G

Q

K

L

R

L

I

D

D

S

K

A

L

V

I

F

F

P

P

G

G

M

I

Q

Q

G

G

G

G

P

P

L

L

E

E

H

H

I

V

I

I

A

A

A

G

K

K

A

A

I

V

A

A

F

F

Q

F

E

E

A

A

L

L

S

Q

D

P

E

E

Y

F

M

K

E

E

Y

Y

I

S

L

R

Q

L

V

V

K

V

K

E

N

N

A

A

S

K

I

R

M

L

A

A

D

E

A

E

F

L

V

A

E

R

K

R

G

G

Y

Y

K

R

L

I

I

V

S

T

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

F

L

L

I

V

D

D

L

L

R

R

N

P

K

K

D

G

L

L

S

T

G

G

K

K

I

E

A

A

E

E

E

E

T

R

L

L

G

D

K

A

V

V

D

G

I

I

T

T

I

V

N

N

K

K

N

N

M

A

V

I

P

P

F

F

D

D

T

P

R

K

S

P

P

P

F

R

V

V

T

T

S

S

G

G

M

I

R

R

V

I

G

G

T

T

A

P

A

A

I

I

T

T

S

T

R

R

G

G

L

F

K

T

E

P

Q

E

E

E

M

M

T

P

K

L

I

V

V

A

D

E

L

L

I

I

D

D

R

R

A

A

L

L

M

L

N

E

H

G

E

P

D

S

E

E

A

A

A

-

L

-

A

-

K

-

I

L

K

R

Q

E

E

E

V

V

N

R

D

R

W

L

M

A

V

L

Q

A

F

H

P

P

L

M

binding serine: Y55 (= Y56), Y56 (= Y57)

8ssyA Room-temperature x-ray structure of thermus thermophilus serine hydroxymethyltransferase (shmt) bound with d-ser in a pseudo- michaelis complex (see paper)
56% identity, 100% coverage: 2:421/422 of query aligns to 1:401/402 of 8ssyA

query
sites
8ssyA

K

K

R

R

D

D

Q

E

V

A

I

L

F

F

D

E

L

L

I

I

Q

A

K

L

E

E

E

E

D

K

R

R

Q

Q

K

R

R

E

G

G

I

L

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

F

T

V

S

S

K

K

Q

Q

V

V

M

R

E

E

A

A

A

V

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y

Y

A

A

E

E

G

G

L

Y

P

P

K

G

K

A

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

V

V

V

I

D

D

D

R

I

V

E

E

Q

S

I

L

A

A

I

I

D

E

R

R

A

A

K

K

E

A

L

L

F

F

G

G

A

A

T

A

W

W

A

A

N

N

V

V

Q

Q

P

P

H

H

S

S

G

G

A

S

Q

Q

A

A

N

N

A

M

A

A

V

V

F

Y

L

M

A

A

C

L

L

M

N

E

P

P

G

G

D

D

H

T

I

L

L

M

G

G

F

M

D

D

L

L

S

A

H

A

G

G

G

G

H

H

L

L

T

T

H

H

G

G

S

S

P

R

V

V

N

N

F

F

S

S

G

G

K

K

N

L

Y

Y

K

K

P

V

H

V

F

S

Y

Y

G

G

V

V

E

R

E

P

E

D

T

T

G

E

I

L

I

I

D

D

M

L

D

E

K

E

V

V

A

R

E

R

K

L

A

A

R

L

E

E

V

H

K

R

P

P

K

K

L

V

I

I

I

V

C

A

G

G

A

A

S

S

A

A

Y

Y

S

P

R

R

D

F

W

W

D

D

Y

F

A

K

R

A

F

F

R

R

E

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

L

Y

L

L

L

V

A

V

D

D

I

M

S

A

H
|
H

P

F

A

A

G

G

L

L

I

V

A

A

R

A

G

G

L

L

L

H

K

P

D

N

P

P

L

L

E

P

H

Y

C

A

H

H

I

V

V

V

T

T

T

S

T

T

T

T

H

H

K
|
K

T

T

L

L

R

R

G

G

T

P

R

R

G

G

G

G

L

L

I

I

M

L

M

S

R

-

E

-

D

-

F

-

D

N

N

D

P

P

F

-

G

-

I

-

K

-

N

-

P

-

K

-

G

-

E

-

L

-

R

-

K

E

M

L

T

G

Q

K

L

R

L

I

D

D

S

K

A

L

V

I

F

F

P

P

G

G

M

I

Q

Q

G

G

G

G

P

P

L

L

E

E

H

H

I

V

I

I

A

A

A

G

K

K

A

A

I

V

A

A

F

F

Q

F

E

E

A

A

L

L

S

Q

D

P

E

E

Y

F

M

K

E

E

Y

Y

I

S

L

R

Q

L

V

V

K

V

K

E

N

N

A

A

S

K

I

R

M

L

A

A

D

E

A

E

F

L

V

A

E

R

K

R

G

G

Y

Y

K

R

L

I

I

V

S

T

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

F

L

L

I

V

D

D

L

L

R

R

N

P

K

K

D

G

L

L

S

T

G

G

K

K

I

E

A

A

E

E

E

E

T

R

L

L

G

D

K

A

V

V

D

G

I

I

T

T

I

V

N

N

K

K

N

N

M

A

V

I

P

P

F

F

D

D

T

P

R

K

S

P

P

P

F

R

V

V

T

T

S

S

G

G

M

I

R
|
R

V

I

G

G

T

T

A

P

A

A

I

I

T

T

S

T

R

R

G

G

L

F

K

T

E

P

Q

E

E

E

M

M

T

P

K

L

I

V

V

A

D

E

L

L

I

I

D

D

R

R

A

A

L

L

M

L

N

E

H

G

E

P

D

S

E

E

A

A

A

-

L

-

A

-

K

-

I

L

K

R

Q

E

E

E

V

V

N

R

D

R

W

L

M

A

V

L

Q

A

F

H

P

P

L

M

binding d-serine: S26 (= S27), H195 (= H196), K221 (= K222), R353 (= R369)

2dkjA Crystal structure of t.Th.Hb8 serine hydroxymethyltransferase
56% identity, 100% coverage: 2:421/422 of query aligns to 1:401/402 of 2dkjA

query
sites
2dkjA

K

K

R

R

D

D

Q

E

V

A

I

L

F

F

D

E

L

L

I

I

Q

A

K

L

E

E

E

E

D

K

R

R

Q

Q

K

R

R

E

G

G

I

L

E

E

L

L

I

I

A

A

S

S

E

E

N

N

F

F

T

V

S

S

K

K

Q

Q

V

V

M

R

E

E

A

A

A

V

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

L

Y

P

P

K

G

K

A

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

V

V

V

I

D

D

D

R

I

V

E

E

Q

S

I

L

A

A

I

I

D

E

R

R

A

A

K

K

E

A

L

L

F

F

G

G

A

A

T

A

W

W

A

A

N

N

V

V

Q

Q

P

P

H

H

S
|
S

G
|
G

A
x
S

Q

Q

A

A

N

N

A

M

A

A

V

V

F

Y

L

M

A

A

C

L

L

M

N

E

P

P

G

G

D

D

H

T

I

L

L

M

G

G

F

M

D

D

L

L

S

A

H

A

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

R

V

V

N

N

F

F

S

S

G

G

K

K

N

L

Y

Y

K

K

P

V

H

V

F

S

Y

Y

G

G

V

V

E

R

E

P

E

D

T

T

G

E

I

L

I

I

D

D

M

L

D

E

K

E

V

V

A

R

E

R

K

L

A

A

R

L

E

E

V

H

K

R

P

P

K

K

L

V

I

I

I

V

C

A

G

G

A

A

S
|
S

A

A

Y

Y

S

P

R

R

D

F

W

W

D

D

Y

F

A

K

R

A

F

F

R

R

E

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

L

Y

L

L

L

V

A

V

D
|
D

I

M

S
x
A

H

H

P

F

A

A

G

G

L

L

I

V

A

A

R

A

G

G

L

L

L

H

K

P

D

N

P

P

L

L

E

P

H

Y

C

A

H

H

I

V

V

V

T

T

T

S

T
|
T

T

T

H
|
H

K
|
K

T

T

L

L

R

R

G

G

T

P

R
|
R

G

G

G

G

L

L

I

I

M

L

M

S

R

-

E

-

D

-

F

-

D

N

N

D

P

P

F

-

G

-

I

-

K

-

N

-

P

-

K

-

G

-

E

-

L

-

R

-

K

E

M

L

T

G

Q

K

L

R

L

I

D

D

S

K

A

L

V

I

F

F

P

P

G

G

M

I

Q

Q

G

G

G

G

P

P

L

L

E

E

H

H

I

V

I

I

A

A

A

G

K

K

A

A

I

V

A

A

F

F

Q

F

E

E

A

A

L

L

S

Q

D

P

E

E

Y

F

M

K

E

E

Y

Y

I

S

L

R

Q

L

V

V

K

V

K

E

N

N

A

A

S

K

I

R

M

L

A

A

D

E

A

E

F

L

V

A

E

R

K

R

G

G

Y

Y

K

R

L

I

I

V

S

T

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

F

L

L

I

V

D

D

L

L

R

R

N

P

K

K

D

G

L

L

S

T

G

G

K

K

I

E

A

A

E

E

E

E

T

R

L

L

G

D

K

A

V

V

D

G

I

I

T

T

I

V

N

N

K

K

N

N

M

A

V

I

P

P

F

F

D

D

T

P

R

K

S

P

P

P

F

R

V

V

T

T

S

S

G

G

M

I

R

R

V

I

G

G

T

T

A

P

A

A

I

I

T

T

S

T

R

R

G

G

L

F

K

T

E

P

Q

E

E

E

M

M

T

P

K

L

I

V

V

A

D

E

L

L

I

I

D

D

R

R

A

A

L

L

M

L

N

E

H

G

E

P

D

S

E

E

A

A

A

-

L

-

A

-

K

-

I

L

K

R

Q

E

E

E

V

V

N

R

D

R

W

L

M

A

V

L

Q

A

F

H

P

P

L

M

active site: Y46 (= Y47), E48 (= E49), D192 (= D193), T218 (= T219), K221 (= K222), R227 (= R228)
binding pyridoxal-5'-phosphate: S88 (= S89), G89 (= G90), S90 (≠ A91), H117 (= H118), S167 (= S168), D192 (= D193), A194 (≠ S195), H220 (= H221), K221 (= K222)

1kl2A Crystal structure of serine hydroxymethyltransferase complexed with glycine and 5-formyl tetrahydrofolate (see paper)
56% identity, 97% coverage: 2:412/422 of query aligns to 6:400/405 of 1kl2A

query
sites
1kl2A

K

Q

R

Q

D

D

Q

P

V

Q

I

V

F

F

D

A

L

A

I

I

Q

E

K

Q

E

E

E

R

D

K

R

R

Q

Q

K

H

R

A

G

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

F

T

V

S

S

K

R

Q

A

V

V

M

M

E

E

A

A

A

Q

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

L

Y

P

P

K

G

K

R

R

R

Y
|
Y

Y
|
Y

G

G

G

G

C

C

E

E

V

Y

V

V

D

D

D

I

I

V

E

E

Q

E

I

L

A

A

I

R

D

E

R

R

A

A

K

K

E

Q

L

L

F

F

G

G

A

A

T

E

W

H

A

A

N

N

V

V

Q

Q

P

P

H

H

S
|
S

G
|
G

A
|
A

Q

Q

A

A

N

N

A

M

A

A

V

V

F

Y

L

F

A

T

C

V

L

L

N

E

P

H

G

G

D

D

H

T

I

V

L

L

G

G

F

M

D

N

L

L

S

S

H

H

G

G

G
|
G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

V

N

Q

Y

Y

K

N

P

F

H

V

F

A

Y

Y

G

G

V

V

E

D

E

P

E

E

T

T

G

H

I

V

I

I

D

D

M

Y

D

D

K

D

V

V

A

R

E

E

K

K

A

A

R

R

E

L

V

H

K

R

P

P

K

K

L

L

I

I

I

V

C

A

G

A

A

A

S
|
S

A

A

Y

Y

S

P

R

R

D

I

W

I

D

D

Y

F

A

A

R

K

F

F

R

R

E

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

L

Y

L

L

L

M

A

V

D
|
D

I

M

S
x
A

H
|
H

P

I

A

A

G

G

L

L

I

V

A

A

R

A

G

G

L

L

L

H

K

P

D

N

P

P

L

V

E

P

H

Y

C

A

H

H

I

F

V

V

T

T

T

T

T
|
T

T

T

H
|
H

K
|
K

T

T

L

L

R

R

G

G

T

P

R
|
R

G

G

G

G

L

M

I

I

M

L

M

C

R

Q

E

E

D

Q

F

F

D

-

N

-

P

-

F

-

G

-

I

-

K

-

N

-

P

-

K

-

G

-

E

-

L

-

R

-

K

-

M

-

T

A

Q

K

L

Q

L

I

D

D

S

K

A

A

V

I

F
|
F

P

P

G

G

M

I

Q

Q

G

G

G

G

P

P

L

L

E

M

H

H

I

V

I

I

A

A

A

A

K

K

A

A

I

V

A

A

F

F

Q

G

E

E

A

A

L

L

S

Q

D

D

E

D

Y

F

M

K

E

A

Y

Y

I

A

L

K

Q

R

V

V

K

V

K

D

N

N

A

A

S

K

I

R

M

L

A

A

D

S

A

A

F

L

V

Q

E

N

K

E

G

G

Y

F

K

T

L

L

I

V

S

S

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

L

L

L

I

V

D

D

L

L

R

R

N

P

K

Q

D

Q

L

L

S

T

G

G

K

K

I

T

A

A

E

E

E

K

T

V

L

L

G

D

K

E

V

V

D

G

I

I

T

T

I

V

N

N

K

K

N
|
N

M

T

V

I

P

P

F

Y

D

D

T

P

R

E

S

S

P

P

F

F

V

V

T

T

S

S

G

G

M

I

R
|
R

V

I

G

G

T

T

A

A

A

A

I

V

T

T

S

T

R

R

G

G

L

F

K

G

E

L

Q

E

E

E

M

M

T

D

K

E

I

I

V

A

D

A

L

I

I

I

D

G

R

L

A

V

L

L

M

K

N

N

H

V

E

G

D

S

E

E

A

Q

A

A

L

L

A

E

K

E

I

A

K

R

Q

Q

E

R

V

V

active site: Y51 (= Y47), E53 (= E49), D197 (= D193), T223 (= T219), K226 (= K222), R232 (= R228)
binding N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid: E53 (= E49), Y60 (= Y56), G121 (= G117), H122 (= H118), S172 (= S168), F251 (= F263), N341 (= N353)
binding glycine: S31 (= S27), Y51 (= Y47), Y61 (= Y57), H200 (= H196), R357 (= R369)
binding pyridoxal-5'-phosphate: S93 (= S89), G94 (= G90), A95 (= A91), H122 (= H118), S172 (= S168), D197 (= D193), A199 (≠ S195), H200 (= H196), T223 (= T219), H225 (= H221), K226 (= K222)

1kl1A Crystal structure of serine hydroxymethyltransferase complexed with glycine (see paper)
56% identity, 97% coverage: 2:412/422 of query aligns to 6:400/405 of 1kl1A

query
sites
1kl1A

K

Q

R

Q

D

D

Q

P

V

Q

I

V

F

F

D

A

L

A

I

I

Q

E

K

Q

E

E

E

R

D

K

R

R

Q

Q

K

H

R

A

G

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

F

T

V

S

S

K

R

Q

A

V

V

M

M

E

E

A

A

A

Q

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

L

Y

P

P

K

G

K

R

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

V

Y

V

V

D

D

D

I

I

V

E

E

Q

E

I

L

A

A

I

R

D

E

R

R

A

A

K

K

E

Q

L

L

F

F

G

G

A

A

T

E

W

H

A

A

N

N

V

V

Q

Q

P

P

H

H

S
|
S

G
|
G

A
|
A

Q

Q

A

A

N

N

A

M

A

A

V

V

F

Y

L

F

A

T

C

V

L

L

N

E

P

H

G

G

D

D

H

T

I

V

L

L

G

G

F

M

D

N

L

L

S

S

H

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

V

N

Q

Y

Y

K

N

P

F

H

V

F

A

Y

Y

G

G

V

V

E

D

E

P

E

E

T

T

G

H

I

V

I

I

D

D

M

Y

D

D

K

D

V

V

A

R

E

E

K

K

A

A

R

R

E

L

V

H

K

R

P

P

K

K

L

L

I

I

I

V

C

A

G

A

A
|
A

S
|
S

A

A

Y

Y

S

P

R

R

D

I

W

I

D

D

Y

F

A

A

R

K

F

F

R

R

E

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

L

Y

L

L

L

M

A

V

D
|
D

I

M

S
x
A

H
|
H

P

I

A

A

G

G

L

L

I

V

A

A

R

A

G

G

L

L

L

H

K

P

D

N

P

P

L

V

E

P

H

Y

C

A

H

H

I

F

V

V

T

T

T

T

T
|
T

T

T

H
|
H

K
|
K

T

T

L

L

R

R

G

G

T

P

R
|
R

G

G

G

G

L

M

I

I

M

L

M

C

R

Q

E

E

D

Q

F

F

D

-

N

-

P

-

F

-

G

-

I

-

K

-

N

-

P

-

K

-

G

-

E

-

L

-

R

-

K

-

M

-

T

A

Q

K

L

Q

L

I

D

D

S

K

A

A

V

I

F

F

P

P

G

G

M

I

Q

Q

G

G

G

G

P

P

L

L

E

M

H

H

I

V

I

I

A

A

A

A

K

K

A

A

I

V

A

A

F

F

Q

G

E

E

A

A

L

L

S

Q

D

D

E

D

Y

F

M

K

E

A

Y

Y

I

A

L

K

Q

R

V

V

K

V

K

D

N

N

A

A

S

K

I

R

M

L

A

A

D

S

A

A

F

L

V

Q

E

N

K

E

G

G

Y

F

K

T

L

L

I

V

S

S

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

L

L

L

I

V

D

D

L

L

R

R

N

P

K

Q

D

Q

L

L

S

T

G

G

K

K

I

T

A

A

E

E

E

K

T

V

L

L

G

D

K

E

V

V

D

G

I

I

T

T

I

V

N

N

K

K

N

N

M

T

V

I

P

P

F

Y

D

D

T

P

R

E

S

S

P

P

F

F

V

V

T

T

S

S

G

G

M

I

R
|
R

V

I

G

G

T

T

A

A

A

A

I

V

T

T

S

T

R

R

G

G

L

F

K

G

E

L

Q

E

E

E

M

M

T

D

K

E

I

I

V

A

D

A

L

I

I

I

D

G

R

L

A

V

L

L

M

K

N

N

H

V

E

G

D

S

E

E

A

Q

A

A

L

L

A

E

K

E

I

A

K

R

Q

Q

E

R

V

V

active site: Y51 (= Y47), E53 (= E49), D197 (= D193), T223 (= T219), K226 (= K222), R232 (= R228)
binding glycine: S31 (= S27), H122 (= H118), R357 (= R369)
binding pyridoxal-5'-phosphate: S93 (= S89), G94 (= G90), A95 (= A91), H122 (= H118), A171 (= A167), S172 (= S168), D197 (= D193), A199 (≠ S195), H200 (= H196), T223 (= T219), H225 (= H221), K226 (= K222)

1kkpA Crystal structure of serine hydroxymethyltransferase complexed with serine (see paper)
56% identity, 97% coverage: 2:412/422 of query aligns to 6:400/405 of 1kkpA

query
sites
1kkpA

K

Q

R

Q

D

D

Q

P

V

Q

I

V

F

F

D

A

L

A

I

I

Q

E

K

Q

E

E

E

R

D

K

R

R

Q

Q

K

H

R

A

G

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

F

T

V

S

S

K

R

Q

A

V

V

M

M

E

E

A

A

A

Q

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

L

Y

P

P

K

G

K

R

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

V

Y

V

V

D

D

D

I

I

V

E

E

Q

E

I

L

A

A

I

R

D

E

R

R

A

A

K

K

E

Q

L

L

F

F

G

G

A

A

T

E

W

H

A

A

N

N

V

V

Q

Q

P

P

H

H

S
|
S

G
|
G

A
|
A

Q

Q

A

A

N

N

A

M

A

A

V

V

F

Y

L

F

A

T

C

V

L

L

N

E

P

H

G

G

D

D

H

T

I

V

L

L

G

G

F

M

D

N

L

L

S

S

H

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

V

N

Q

Y

Y

K

N

P

F

H

V

F

A

Y

Y

G

G

V

V

E

D

E

P

E

E

T

T

G

H

I

V

I

I

D

D

M

Y

D

D

K

D

V

V

A

R

E

E

K

K

A

A

R

R

E

L

V

H

K

R

P

P

K

K

L

L

I

I

I

V

C

A

G

A

A

A

S
|
S

A

A

Y

Y

S

P

R

R

D

I

W

I

D

D

Y

F

A

A

R

K

F

F

R

R

E

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

L

Y

L

L

L

M

A

V

D
|
D

I

M

S
x
A

H
|
H

P

I

A

A

G

G

L

L

I

V

A

A

R

A

G

G

L

L

L

H

K

P

D

N

P

P

L

V

E

P

H

Y

C

A

H

H

I

F

V

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

T

T

L

L

R

R

G

G

T

P

R
|
R

G

G

G

G

L

M

I

I

M

L

M

C

R

Q

E

E

D

Q

F

F

D

-

N

-

P

-

F

-

G

-

I

-

K

-

N

-

P

-

K

-

G

-

E

-

L

-

R

-

K

-

M

-

T

A

Q

K

L

Q

L

I

D

D

S

K

A

A

V

I

F

F

P

P

G

G

M

I

Q

Q

G

G

G

G

P

P

L

L

E

M

H

H

I

V

I

I

A

A

A

A

K

K

A

A

I

V

A

A

F

F

Q

G

E

E

A

A

L

L

S

Q

D

D

E

D

Y

F

M

K

E

A

Y

Y

I

A

L

K

Q

R

V

V

K

V

K

D

N

N

A

A

S

K

I

R

M

L

A

A

D

S

A

A

F

L

V

Q

E

N

K

E

G

G

Y

F

K

T

L

L

I

V

S

S

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

L

L

L

I

V

D

D

L

L

R

R

N

P

K

Q

D

Q

L

L

S

T

G

G

K

K

I

T

A

A

E

E

E

K

T

V

L

L

G

D

K

E

V

V

D

G

I

I

T

T

I

V

N

N

K

K

N

N

M

T

V

I

P

P

F

Y

D

D

T

P

R

E

S

S

P

P

F

F

V

V

T

T

S

S

G

G

M

I

R
|
R

V

I

G

G

T

T

A

A

A

A

I

V

T

T

S

T

R

R

G

G

L

F

K

G

E

L

Q

E

E

E

M

M

T

D

K

E

I

I

V

A

D

A

L

I

I

I

D

G

R

L

A

V

L

L

M

K

N

N

H

V

E

G

D

S

E

E

A

Q

A

A

L

L

A

E

K

E

I

A

K

R

Q

Q

E

R

V

V

active site: Y51 (= Y47), E53 (= E49), D197 (= D193), T223 (= T219), K226 (= K222), R232 (= R228)
binding pyridoxal-5'-phosphate: S93 (= S89), G94 (= G90), A95 (= A91), H122 (= H118), S172 (= S168), D197 (= D193), A199 (≠ S195), H200 (= H196), K226 (= K222)
binding serine: S31 (= S27), H122 (= H118), R357 (= R369)

1kkjA Crystal structure of serine hydroxymethyltransferase from b.Stearothermophilus (see paper)
56% identity, 97% coverage: 2:412/422 of query aligns to 6:400/405 of 1kkjA

query
sites
1kkjA

K

Q

R

Q

D

D

Q

P

V

Q

I

V

F

F

D

A

L

A

I

I

Q

E

K

Q

E

E

E

R

D

K

R

R

Q

Q

K

H

R

A

G

K

I

I

E

E

L

L

I

I

A

A

S

S

E

E

N

N

F

F

T

V

S

S

K

R

Q

A

V

V

M

M

E

E

A

A

A

Q

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

L

Y

P

P

K

G

K

R

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

V

Y

V

V

D

D

D

I

I

V

E

E

Q

E

I

L

A

A

I

R

D

E

R

R

A

A

K

K

E

Q

L

L

F

F

G

G

A

A

T

E

W

H

A

A

N

N

V

V

Q

Q

P

P

H

H

S
|
S

G
|
G

A
|
A

Q

Q

A

A

N

N

A

M

A

A

V

V

F

Y

L

F

A

T

C

V

L

L

N

E

P

H

G

G

D

D

H

T

I

V

L

L

G

G

F

M

D

N

L

L

S

S

H

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

V

N

Q

Y

Y

K

N

P

F

H

V

F

A

Y

Y

G

G

V

V

E

D

E

P

E

E

T

T

G

H

I

V

I

I

D

D

M

Y

D

D

K

D

V

V

A

R

E

E

K

K

A

A

R

R

E

L

V

H

K

R

P

P

K

K

L

L

I

I

I

V

C

A

G

A

A

A

S
|
S

A

A

Y

Y

S

P

R

R

D

I

W

I

D

D

Y

F

A

A

R

K

F

F

R

R

E

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

L

Y

L

L

L

M

A

V

D
|
D

I

M

S
x
A

H
|
H

P

I

A

A

G

G

L

L

I

V

A

A

R

A

G

G

L

L

L

H

K

P

D

N

P

P

L

V

E

P

H

Y

C

A

H

H

I

F

V

V

T

T

T

T

T
|
T

T

T

H
|
H

K
|
K

T

T

L

L

R

R

G

G

T

P

R
|
R

G

G

G

G

L

M

I

I

M

L

M

C

R

Q

E

E

D

Q

F

F

D

-

N

-

P

-

F

-

G

-

I

-

K

-

N

-

P

-

K

-

G

-

E

-

L

-

R

-

K

-

M

-

T

A

Q

K

L

Q

L

I

D

D

S

K

A

A

V

I

F

F

P

P

G

G

M

I

Q

Q

G

G

G

G

P

P

L

L

E

M

H

H

I

V

I

I

A

A

A

A

K

K

A

A

I

V

A

A

F

F

Q

G

E

E

A

A

L

L

S

Q

D

D

E

D

Y

F

M

K

E

A

Y

Y

I

A

L

K

Q

R

V

V

K

V

K

D

N

N

A

A

S

K

I

R

M

L

A

A

D

S

A

A

F

L

V

Q

E

N

K

E

G

G

Y

F

K

T

L

L

I

V

S

S

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

L

L

L

I

V

D

D

L

L

R

R

N

P

K

Q

D

Q

L

L

S

T

G

G

K

K

I

T

A

A

E

E

E

K

T

V

L

L

G

D

K

E

V

V

D

G

I

I

T

T

I

V

N

N

K

K

N

N

M

T

V

I

P

P

F

Y

D

D

T

P

R

E

S

S

P

P

F

F

V

V

T

T

S

S

G

G

M

I

R

R

V

I

G

G

T

T

A

A

A

A

I

V

T

T

S

T

R

R

G

G

L

F

K

G

E

L

Q

E

E

E

M

M

T

D

K

E

I

I

V

A

D

A

L

I

I

I

D

G

R

L

A

V

L

L

M

K

N

N

H

V

E

G

D

S

E

E

A

Q

A

A

L

L

A

E

K

E

I

A

K

R

Q

Q

E

R

V

V

active site: Y51 (= Y47), E53 (= E49), D197 (= D193), T223 (= T219), K226 (= K222), R232 (= R228)
binding pyridoxal-5'-phosphate: S93 (= S89), G94 (= G90), A95 (= A91), H122 (= H118), S172 (= S168), D197 (= D193), A199 (≠ S195), H200 (= H196), T223 (= T219), H225 (= H221), K226 (= K222)

2vmyA Crystal structure of f351gbsshmt in complex with gly and fthf (see paper)
56% identity, 97% coverage: 2:412/422 of query aligns to 6:400/405 of 2vmyA

query
sites
2vmyA

K

Q

R

Q

D

D

Q

P

V

Q

I

V

F

F

D

A

L

A

I

I

Q

E

K

Q

E

E

E

R

D

K

R

R

Q

Q

K

H

R

A

G

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

F

T

V

S

S

K

R

Q

A

V

V

M

M

E

E

A

A

A

Q

G

G

S

S

V

V

L

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

L

Y

P

P

K

G

K

R

R

R

Y
|
Y

Y
|
Y

G

G

G

G

C

C

E

E

V

Y

V

V

D

D

D

I

I

V

E

E

Q

E

I

L

A

A

I

R

D

E

R

R

A

A

K

K

E

Q

L

L

F

F

G

G

A

A

T

E

W

H

A

A

N

N

V

V

Q

Q

P

P

H

H

S
|
S

G
|
G

A
|
A

Q

Q

A

A

N

N

A

M

A

A

V

V

F

Y

L

F

A

T

C

V

L

L

N

E

P

H

G

G

D

D

H

T

I

V

L

L

G

G

F

M

D

N

L
|
L

S

S

H

H

G

G

G
|
G

H
|
H

L
|
L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

V

N

Q

Y

Y

K

N

P

F

H

V

F

A

Y

Y

G

G

V

V

E

D

E

P

E

E

T

T

G

H

I

V

I

I

D

D

M

Y

D

D

K

D

V

V

A

R

E

E

K

K

A

A

R

R

E

L

V

H

K

R

P

P

K

K

L

L

I

I

I

V

C

A

G

A

A

A

S
|
S

A

A

Y

Y

S

P

R

R

D

I

W

I

D

D

Y

F

A

A

R

K

F

F

R

R

E

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

L

Y

L

L

L

M

A

V

D
|
D

I

M

S
x
A

H
|
H

P

I

A

A

G

G

L

L

I

V

A

A

R

A

G

G

L

L

L

H

K

P

D

N

P

P

L

V

E

P

H

Y

C

A

H

H

I

F

V

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

T

T

L

L

R

R

G

G

T

P

R
|
R

G

G

G

G

L

M

I

I

M

L

M

C

R

Q

E

E

D

Q

F

F

D

-

N

-

P

-

F

-

G

-

I

-

K

-

N

-

P

-

K

-

G

-

E

-

L

-

R

-

K

-

M

-

T

A

Q

K

L

Q

L

I

D

D

S
x
K

A

A

V

I

F
|
F

P

P

G

G

M

I

Q

Q

G

G

G
|
G

P

P

L

L

E

M

H

H

I

V

I

I

A

A

A

A

K

K

A

A

I

V

A

A

F

F

Q

G

E

E

A

A

L

L

S

Q

D

D

E

D

Y

F

M

K

E

A

Y

Y

I

A

L

K

Q

R

V

V

K

V

K

D

N

N

A

A

S

K

I

R

M

L

A

A

D

S

A

A

F