SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_1535 FitnessBrowser__Cola:Echvi_1535 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P39633 Catabolic NAD-specific glutamate dehydrogenase RocG; NAD-GDH; Glutamate dehydrogenase; GlutDH; Trigger enzyme RocG; EC 1.4.1.2 from Bacillus subtilis (strain 168) (see 2 papers)
46% identity, 94% coverage: 26:423/425 of query aligns to 28:422/424 of P39633

query
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P39633

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E93 (= E91) mutation to K: Reduces the affinity for glutamate and ammonium.
D122 (≠ N120) mutation to N: Unable to control gltAB expression via an inhibitory interactions with the transcriptional regulator GltC. Reduces the affinity for glutamate and ammonium.
Q144 (≠ D142) mutation to R: Increase of thermostability 20 degrees Celsius higher than that of the wild-type.
Y158 (≠ G156) mutation to H: Reduces the affinity for glutamate and ammonium.
S234 (= S232) mutation to R: Reduces the affinity for glutamate and ammonium.
A324 (≠ G325) mutation to R: No effect.

Sites not aligning to the query:

27 E→F: Increase of thermostability 8 degrees Celsius higher than that of the wild-type.

8xcoA Cryo-em structure of glutamate dehydrogenase from thermococcus profundus incorporating NADPH in the initial stage of reaction
49% identity, 96% coverage: 14:423/425 of query aligns to 2:412/416 of 8xcoA

query
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8xcoA

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binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T188 (= T198), Y217 (≠ F227), G218 (= G228), N219 (= N229), A220 (≠ V230), D241 (= D250), S242 (≠ I251), K261 (≠ R270), I294 (≠ V305)

8xd5A Cryo-em structure of glutamate dehydrogenase from thermococcus profundus in complex with NADP and glu in the steady stage of reaction
49% identity, 96% coverage: 14:423/425 of query aligns to 5:415/419 of 8xd5A

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8xd5A

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binding gamma-l-glutamic acid: K69 (= K78), M90 (= M99), K105 (= K114), A143 (= A152), D145 (= D154), S351 (= S359)
binding nadp nicotinamide-adenine-dinucleotide phosphate: R73 (= R82), D145 (= D154), V146 (≠ M155), Y147 (≠ G156), T191 (= T198), Y220 (≠ F227), G221 (= G228), N222 (= N229), A223 (≠ V230), D244 (= D250), S245 (≠ I251), K264 (≠ R270), N281 (≠ D288), A295 (= A303), A296 (= A304), I297 (≠ V305), N319 (= N327), N344 (= N352)

8xcsA Cryo-em structure of glutamate dehydrogenase from thermococcus profundus in complex with NADPH, akg and nh4 in the initial stage of reaction
49% identity, 96% coverage: 14:423/425 of query aligns to 4:414/418 of 8xcsA

query
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8xcsA

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A

M

V

E

K

R

Q

F

L

N

E

I

R

A

A

E

Q

K

Y

L

M

G

D

L

I

S

S

E

E

V

A

Y

L

N

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V

W

L

L

K

K

N

K

P

P

A

M

K

R

Q

I

V

V

I

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V

V

S

S

L

V

P

P

I

I

T

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M

M

D

D

N

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G

G

K

S

I

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K

K

V

V

F

F

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G

G

I

F

R

R

V

V

V

Q

H

H

S

N

N

W

I

A

L

R

G

G

P

P

A

T

K
|
K

G

G

G
|
G

I

I

R
|
R

F

W

A

H

P

P

D

A

V

E

H

T

I

L

D

S

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T

V

V

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K

A

A

L

L

A

A

A

T

W

W

M
|
M

T

T

W

W

K
|
K

C

V

A

A

V

V

D

D

I

L

P

P

Y

Y

G

G

K
|
K

G

G

V

I

R

I

C

V

N

N

P

P

R
x
K

E

E

M

L

S

S

P

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G

R

E

E

I

Q

E

E

R

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L

L

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A

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R

A

A

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Y

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A

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V

I

Y

D

D

V

V

F

I

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W

K

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D

D

I

I

P

P

A
|
A

P
|
P

D
|
D

M
x
V

G
x
Y

T

T

G

N

P

P

K

K

E

I

M

M

A

G

W

W

L

M

M

M

D

D

E

E

Y

Y

S

E

-

T

-

I

-

M

K

R

A

R

K

K

G

G

T

P

T

A

V

F

N

-

A

G

V

V

V

I

T

T

G

G

K

K

P

P

L

L

V

S

L

I

G

G

S

S

L

L

G

G

R

R

T

G

E

T

A

A

T
|
T

G

A

R

Q

G

G

V

A

M

I

V

F

S

T

A

I

L

R

A

E

A

A

M

A

E

K

K

A

L

L

K

G

I

I

N

D

P

L

F

K

Q

G

A

K

T

K

C

I

A

A

V

V

Q

Q

G

G

F
x
Y

G
|
G

N
|
N

V
x
A

G

G

S

Y

W

Y

A

T

S

A

A

K

L

L

L

A

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K

E

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Q

-

L

G

G

L

M

K

T

V

V

A

A

V

V

S

S

D
|
D

I
x
S

S

R

G

G

A

G

Y

I

Y

Y

N

N

A

P

N

D

G

G

I

L

N

D

I

P

Q

D

K

E

A

V

I

L

A

K

Y

W

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x
K

D

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G

-

N

E

K

H

G

G

T

S

L

V

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K

G

D

F

F

D

P

G

G

A

A

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T

K

N

L

I

S

T

D

N

P

E

M

-

E

E

L

L

E

E

L

L

K

E

V

V

D

D

V

V

L

L

V

A

P

P

A

A

A
|
A

V
x
I

E

E

D

E

V

V

I

I

T

T

K

E

A

K

N

N

V

A

D

D

K

N

I

I

N

K

A

A

R

K

L

I

I

V

V

A

E

E

G

V

A

A

N
|
N

G

G

P

P

T

V

S

T

F

P

N

E

A

A

D

D

K

D

I

I

I

L

N

R

D

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K

K

G

G

I

I

M

L

V

Q

V

I

P

P

D

D

I

F

L

L

A

C

N

N

A

A

G

G

G
|
G

V

V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

I

L

N

G

G

Y

Y

K

Y

W

W

T

T

A

E

E

E

R

E

V

V

N

R

R

E

R

K

S

L

D

D

R

K

I

K

M

M

K

T

E

K

A

A

F

F

D

W

Q

E

V

V

Y

Y

K

N

V

T

S

H

V

K

K

D

H

K

G

N

V

I

P

H

M

M

R

R

I

D

A

A

Y

Y

I

V

V

V

A

A

I

V

D

S

K

R

V

V

A

Y

Q

Q

T

A

Y

M

Q

K

F

D

R

R

G

G

binding 2-oxoglutaric acid: K68 (= K78), G70 (= G80), M89 (= M99), K92 (= K102), K104 (= K114), A142 (= A152), D144 (= D154), G346 (= G355), S350 (= S359)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R72 (= R82), K112 (≠ R122), P143 (= P153), D144 (= D154), V145 (≠ M155), Y146 (≠ G156), T190 (= T198), Y219 (≠ F227), G220 (= G228), N221 (= N229), A222 (≠ V230), D243 (= D250), S244 (≠ I251), K263 (≠ R270), A295 (= A304), I296 (≠ V305), N318 (= N327)

P50735 Cryptic catabolic NAD-specific glutamate dehydrogenase GudB; NAD-GDH; EC 1.4.1.2 from Bacillus subtilis (strain 168) (see paper)
45% identity, 97% coverage: 10:423/425 of query aligns to 12:425/427 of P50735

query
sites
P50735

K

E

D

D

K

K

E

L

N

D

P

V

L

L

E

K

S

S

M

T

M

Q

E

T

R

V

F

I

N

H

I

K

A

A

A

L

E

E

K

K

L

L

G

G

L

Y

S

P

E

E

V

V

Y

Y

N

E

V

L

L

L

K

K

N

E

P

P

A

M

K

R

Q

L

V

L

I

T

V

V

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K

L

I

P

P

I

V

T

R

M

M

D

D

N

D

G

G

K

S

I

V

K

K

V

I

F

F

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T

G

G

I

Y

R

R

V

A

V

Q

H

H

S

N

N

D

I

S

L

V

G

G

P

P

A

T

K

K

G

G

I

I

R

R

F

F

A

H

P

P

D

N

V

V

-

T

-

E

-

K

H

E

I

V

D

K

E

A

V
|
V

R
x
K

A
|
A

L

L

A

S

A

I

W

W

M

M

T

S

W

L

K

K

C

C

A

G

V

I

D

D

I

L

P

P

Y

Y

G

G

K

K

G

G

V

I

R

V

C

C

N

D

P

P

R

R

E

D

M

M

S

S

P

F

G

R

E

E

I

L

E

E

R

R

L

L

V

S

R

R

A

G

Y

Y

T

V

L

R

A

A

M

I

I

S

D

Q

V

I

F

V

G

G

P

P

D

T

K

K

D

D

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V

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P

A

A

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V

G

F

T

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G

N

P

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K

Q

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M

M

A

A

W

W

L

M

M

M

D

D

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Y

Y

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S

K

R

A

I

K

D

G

E

T

F

T

N

V

S

N

P

A

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V

F

V

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G

G

K

K

P

P

L

L

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V

L

L

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S

L

H

G

G

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A

A

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A

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G

G

V

V

M

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V

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C

A

I

L

K

A

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A

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A

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K

K

K

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K

G

I

I

N

D

P

I

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K

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G

A

A

T

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C

V

A

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V

V

Q

Q

G

G

F

F

G

G

N

N

V

A

G

G

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S

W

Y

A

L

S

A

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K

L

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G

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I

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D

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Y

A

L

I

L

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Y

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R

R

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S

N

F

K

G

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L

T

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-

A

-

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M

Q

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L

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L

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C

D

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L

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P

A

A

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N

V

Q

I

I

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T

K

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A

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N

N

V

A

D

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K

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I

I

N

R

A

A

R

K

L

I

I

V

V

V

E

E

G

A

A

A

N

N

G

G

P

P

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T

S

T

F

L

N

E

A

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D

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K

K

I

I

I

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N

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D

D

K

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M

L

V

L

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V

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P

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D

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V

L

L

A

A

N

S

A

A

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

N

L

Q

G

G

Y

F

K

Y

W

W

T

S

A

E

E

E

R

E

V

V

N

E

R

E

R

K

S

L

D

E

R

K

I

M

M

M

K

V

E

K

A

S

F

F

D

N

Q

N

V

I

Y

Y

K

E

V

M

S

A

V

N

K

N

H

R

G

R

V

I

P

D

M

M

R

R

I

L

A

A

Y

Y

I

M

V

V

A

G

I

V

D

R

K

K

V

M

A

A

Q

E

T

A

Y

S

Q

R

F

F

R

R

G

G

VKA 97:99 (≠ VRA 92:94) mutation Missing: In gudB1; gains glutamate dehydrogenase activity, restores growth on proline, arginine, ornithine.

6yeiA Arabidopsis thaliana glutamate dehydrogenase isoform 1 in complex with NAD (see paper)
47% identity, 96% coverage: 14:423/425 of query aligns to 1:407/409 of 6yeiA

query
sites
6yeiA

N

N

P

A

L

L

E

A

S

A

M

T

M

N

E

R

R

N

F

F

N

K

I

L

A

A

E

R

K

L

L

L

G

G

L

L

S

D

E

S

E

K

V

L

Y

E

N

K

V
x
S

L
|
L

K

L

N
x
I

P
|
P

A

F

K

R

Q

E

V

I

I

K

V

V

S

E

L

C

P

T

I

I

T

P

M

K

D

D

N

D

G

G

K

T

I

L

K

A

V

S

F

F

E

V

G

G

I

F

R

R

V

V

V

Q

H

H

S

D

N

N

I

A

L

R

G

G

P

P

A

M

K

K

G

G

I

I

R

R

F

Y

A

H

P

P

D

E

V

V

H

D

I

P

D

D

E

E

V

V

R

N

A

A

L

L

A

A

A

Q

W

L

M

M

T

T

W

W

K

K

C

T

A

A

V

V

V

A

D

K

I

I

P

P

Y

Y

G

G

G

A

K

K

G

G

V

I

R

G

C

C

N

D

P

P

R

S

E

K

M

L

S

S

P

I

G

S

E

E

I

L

E

E

R

R

L

L

V

T

R

R

A

V

Y

F

T

T

L

Q

A

K

M

I

I

H

D

D

V

L

F

I

G

G

P

I

D

H

K

T

D

D

I

V

P

P

A

A

P

P

D

D

M

M

G

G

T

T

G

G

P

P

K

Q

E

T

M

M

A

A

W

W

L

I

M

L

D

D

E

E

Y

Y

S

S

K

K

A

F

K

H

G

G

T

Y

T

S

V

-

N

P

A

A

V

V

T

T

G

G

K

K

P

P

L

I

V

D

L

L

G

G

S

S

L

L

G

G

R

R

T

D

E

A

A

A

T
|
T

G

G

R

R

G

G

V

V

M

M

V

F

S

G

A

T

L

E

A

A

A

L

M

L

E

N

K

E

L

H

K

G

I

K

N

T

P

I

F

S

Q

G

A

Q

T

R

C

F

A

V

V

I

Q

Q

G

G

F
|
F

G
|
G

N
|
N

V
|
V

G

G

S

S

W

W

A

A

S

A

A

K

L

L

L

I

E

S

E

E

R

K

G

G

L

G

K

K

V

I

V

V

A

A

V

V

S

S

D
|
D

I
|
I

S

T

G

G

A

A

Y

I

Y

K

N

N

A

K

N

D

G

G

I

I

N

D

I

I

Q

P

K

A

A

L

I

L

A

K

Y

H

R

T

D

K

G

E

N

H

K

R

G

G

T

-

L

V

E

K

G

G

F

F

D

D

G

G

A

A

E

D

K

P

L

I

S

-

D

D

P

P

M

N

E

S

L

I

L

L

E

V

L

E

K

D

V

C

D

D

V

I

L

L

V

V

P

P

A

A

A
|
A

V
x
L

E

G

D

G

V

V

I

I

T

N

K

R

A

E

N

N

V

A

D

N

K

E

I

I

N

K

A

A

R

K

L

F

I

I

V

I

E

E

G

A

A
|
A

N
|
N

G

H

P

P

T

T

S

D

F

P

N

D

A

A

D

D

K

E

I

I

I

L

N

S

D

K

K

K

G

G

I

V

M

V

V

I

V

L

P

P

D

D

I

I

L

Y

A

A

N
|
N

A

S

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

I

L

Q

G

G

Y

F

K

M

W

W

T

E

A

E

E

E

R

K

V

V

N

N

R

D

R

E

S

L

D

K

R

T

I

Y

M

M

K

T

E

R

A

S

F

F

D

K

Q

D

V

L

Y

K

K

E

V

M

S

C

V

K

K

T

H

H

G

S

V

C

P

D

M

L

R

R

I

M

A

G

A

A

Y

F

I

T

V

L

A

G

I

V

D

N

K

R

V

V

A

A

Q

Q

T

A

Y

T

Q

I

F

L

R

R

G

G

binding potassium ion: S26 (≠ V39), L27 (= L40), I29 (≠ N42), P30 (= P43)
binding nicotinamide-adenine-dinucleotide: T184 (= T198), F213 (= F227), G214 (= G228), N215 (= N229), V216 (= V230), D236 (= D250), I237 (= I251), A288 (= A304), L289 (≠ V305), A310 (= A326), N311 (= N327), N336 (= N352)

3aogA Crystal structure of glutamate dehydrogenase (gdhb) from thermus thermophilus (glu bound form)
48% identity, 96% coverage: 15:423/425 of query aligns to 12:418/421 of 3aogA

query
sites
3aogA

P

P

L

W

E

E

S

I

M

F

M

T

E

E

R

Q

F

V

N

D

I

R

A

V

E

P

K

Y

L

L

G

G

L

R

S

L

E

A

E

P

V

L

Y

A

N

E

V

S

L

L

K

K

N

R

P

P

A

K

K

R

Q

V

V

L

I

I

V

V

S

D

L

V

P

P

I

V

T

R

M

L

D

D

N

D

G

G

K

S

I

V

K

A

V

Y

F

F

E

E

G

G

I

Y

R

R

V

V

V

H

H

H

S

N

N

T

I
x
A

L

R

G

G

P

P

A

A

K

K

G

G

G
|
G

I

V

R

R

F

Y

A

H

P

P

D

E

V

V

H

T

I

L

D

S

E

E

V

V

R

M

A

A

L

L

A

A

A

G

W

W

M
|
M

T

T

W

I

K

K

C

N

A

A

V

A

V

V

D

G

I

L

P

P

Y

Y

G

G

K
|
K

G

G

V

I

R

R

C

V

N

D

P

P

R

R

E

K

M

L

S

S

P

P

G

G

E

E

I

L

E

E

R

R

L

L

V

T

R

R

A

R

Y

Y

T

T

L

S

A

E

M

I

I

G

D

I

V

L

F

L

G

G

P

P

D

D

K

R

D

D

I

I

P

P

A

A

P
|
P

D
|
D

M

V

G

N

T

T

G

G

P

E

K

R

E

E

M

M

A

A

W

W

L

M

M

M

D
|
D

E

T

Y

Y

S

S

K
x
M

A

N

K

V

G

G

T

R

T

T

V

V

N

P

A

G

V

V

T

T

G

G

K

K

P

P

L

I

V

A

L

L

G

G

S

S

L

L

G

G

R

R

T

R

E

D

A

A

T

T

G

G

R

R

G

G

V

V

M

F

V

I

S

T

A

A

L

A

A

A

M

A

E

E

K

K

L

I

K

G

I

L

N

Q

P

V

F

E

Q

G

A

A

T

R

C

V

A

A

V

I

Q

Q

G

G

F

F

G

G

N

N

V

V

G

G

S

N

W

A

A

A

S

A

A

R

L

A

L

F

E

H

E

D

R

H

G

G

L

A

K

R

V

V

A

A

V

V

S

Q

D

D

I

H

S

T

G

G

A

T

Y

V

Y

Y

N

N

A

E

N

A

G

G

I

I

N

D

I

P

Q

Y

K

D

A

L

I

L

A

R

Y

H

R

V

D

Q

G

-

N

E

K

F

G

G

T

G

L

V

E

R

G

G

F

Y

D

P

G

K

A

A

E

E

K

P

L

L

S

P

D

-

P

A

M

A

E

D

L

F

L

W

E

G

L

L

K

P

V

V

D

E

V

F

L

L

V

V

P

P

A

A

V

L

E

E

D

K

V

Q

I

I

T

T

K

E

A

Q

N

N

V

A

D

W

K

R

I

I

N

R

A

A

R

R

L

I

I

V

V

A

E

E

G

G

A

A

N

N

G

G

P

P

T

T

S

T

F

P

N

A

A

A

D

D

K

D

I

I

I

L

N

L

D

E

K

K

G

G

I

V

M

L

V

V

P

P

D

D

I

V

L

I

A

A

N

N

A

A

G

G

V

V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

D

R

F

L

N

G

S

Y

Y

K

F

W

W

T

T

A

E

E

E

R

E

V

I

N

N

R

A

R

R

S

L

D

E

R

R

I

V

M

L

K

R

E

N

A

A

F

F

D

E

Q

A

V

V

Y

W

K

Q

V

V

S

A

V

Q

K

E

H

K

G

K

V

I

P

P

M

L

R

R

I

T

A

A

Y

Y

I

V

V

V

A

A

I

A

D

T

K

R

V

V

A

L

Q

E

T

A

Y

R

Q

A

F

L

R
|
R

G
|
G

active site: K111 (= K114), D151 (= D154)
binding glutamic acid: A70 (≠ I73), G77 (= G80), M96 (= M99), K111 (= K114), P150 (= P153), D151 (= D154), D164 (= D167), M168 (≠ K171), S354 (= S359), R417 (= R422), G418 (= G423)

Sites not aligning to the query:

binding glutamic acid: 419, 420

6yehA Arabidopsis thaliana glutamate dehydrogenase isoform 1 in apo form (see paper)
47% identity, 96% coverage: 14:423/425 of query aligns to 1:407/410 of 6yehA

query
sites
6yehA

N

N

P

A

L

L

E

A

S

A

M

T

M

N

E

R

R

N

F

F

N

K

I

L

A

A

E

R

K

L

L

L

G

G

L

L

S

D

E

S

E

K

V

L

Y

E

N

K

V
x
S

L
|
L

K

L

N
x
I

P
|
P

A

F

K

R

Q

E

V

I

I

K

V

V

S

E

L

C

P

T

I

I

T

P

M

K

D

D

N

D

G

G

K

T

I

L

K

A

V

S

F

F

E

V

G

G

I

F

R

R

V

V

V

Q

H

H

S
x
D

N

N

I

A

L

R

G

G

P

P

A

M

K

K

G

G

I

I

R

R

F

Y

A

H

P

P

D

E

V

V

H

D

I

P

D

D

E

E

V

V

R

N

A

A

L

L

A

A

A

Q

W

L

M

M

T

T

W

W

K

K

C

T

A

A

V

V

V

A

D

K

I

I

P

P

Y

Y

G

G

G

A

K

K

G

G

V

I

R

G

C

C

N

D

P

P

R

S

E

K

M

L

S

S

P

I

G

S

E

E

I

L

E

E

R

R

L

L

V

T

R

R

A

V

Y

F

T

T

L

Q

A

K

M

I

I

H

D

D

V

L

F

I

G

G

P

I

D

H

K

T

D

D

I

V

P

P

A

A

P

P

D

D

M

M

G

G

T

T

G

G

P

P

K

Q

E

T

M

M

A

A

W

W

L

I

M

L

D

D

E

E

Y

Y

S

S

K

K

A

F

K

H

G

G

T

Y

T

S

V

-

N

P

A

A

V

V

T

T

G

G

K

K

P

P

L

I

V

D

L

L

G

G

S

S

L

L

G

G

R

R

T

D

E

A

A

A

T

T

G

G

R

R

G

G

V

V

M

M

V

F

S

G

A

T

L

E

A

A

A

L

M

L

E

N

K

E

L

H

K

G

I

K

N

T

P

I

F

S

Q

G

A

Q

T

R

C

F

A

V

V

I

Q

Q

G

G

F

F

G

G

N

N

V

V

G

G

S

S

W

W

A

A

S

A

A

K

L

L

L

I

E

S

E

E

R

K

G

G

L

G

K

K

V

I

V

V

A

A

V

V

S

S

D

D

I

I

S

T

G

G

A

A

Y

I

Y

K

N

N

A

K

N

D

G

G

I

I

N

D

I

I

Q

P

K

A

A

L

I

L

A

K

Y

H

R

T

D

K

G

E

N

H

K

R

G

G

T

-

L

V

E

K

G

G

F

F

D

D

G

G

A

A

E

D

K

P

L

I

S

-

D

D

P

P

M

N

E

S

L

I

L

L

E

V

L

E

K

D

V

C

D

D

V

I

L

L

V

V

P

P

A

A

V

L

E

G

D

G

V

V

I

I

T

N

K

R

A

E

N

N

V

A

D

N

K

E

I

I

N

K

A

A

R

K

L

F

I

I

V

I

E

E

G

A

A

A

N

N

G

H

P

P

T

T

S

D

F

P

N

D

A

A

D

D

K

E

I

I

I

L

N

S

D

K

K

K

G

G

I

V

M

V

V

I

V

L

P

P

D

D

I

I

L

Y

A

A

N

N

A

S

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

I

L

Q

G

G

Y

F

K

M

W

W

T

E

A

E

E

E

R

K

V

V

N

N

R

D

R

E

S

L

D

K

R

T

I

Y

M

M

K

T

E

R

A

S

F

F

D

K

Q

D

V

L

Y

K

K

E

V

M

S

C

V

K

K

T

H

H

G

S

V

C

P

D

M

L

R

R

I

M

A

G

A

A

Y

F

I

T

V

L

A

G

I

V

D

N

K

R

V

V

A

A

Q

Q

T

A

Y

T

Q

I

F

L

R

R

G

G

binding potassium ion: S26 (≠ V39), L27 (= L40), I29 (≠ N42), P30 (= P43), D58 (≠ S71)

6yeiF Arabidopsis thaliana glutamate dehydrogenase isoform 1 in complex with NAD (see paper)
47% identity, 96% coverage: 14:423/425 of query aligns to 2:408/410 of 6yeiF

query
sites
6yeiF

N

N

P

A

L

L

E

A

S

A

M

T

M

N

E

R

R

N

F

F

N

K

I

L

A

A

E

R

K

L

L

L

G

G

L

L

S

D

E

S

E

K

V

L

Y

E

N

K

V
x
S

L
|
L

K

L

N
x
I

P
|
P

A
x
F

K

R

Q

E

V

I

I

K

V

V

S

E

L

C

P

T

I

I

T

P

M

K

D

D

N

D

G

G

K

T

I

L

K

A

V

S

F

F

E

V

G

G

I

F

R

R

V

V

V

Q

H

H

S

D

N

N

I

A

L

R

G

G

P

P

A

M

K
|
K

G

G

G
|
G

I

I

R
|
R

F

Y

A

H

P

P

D

E

V

V

H

D

I

P

D

D

E

E

V

V

R

N

A

A

L

L

A

A

A

Q

W

L

M
|
M

T

T

W

W

K
|
K

C

T

A

A

V

V

V

A

D

K

I

I

P

P

Y

Y

G

G

G

A

K
|
K

G

G

V

I

R

G

C

C

N

D

P

P

R

S

E

K

M

L

S

S

P

I

G

S

E

E

I

L

E

E

R

R

L

L

V

T

R

R

A

V

Y

F

T

T

L

Q

A

K

M

I

I

H

D

D

V

L

F

I

G

G

P

I

D

H

K

T

D

D

I

V

P

P

A
|
A

P

P

D
|
D

M
|
M

G

G

T

T

G

G

P

P

K

Q

E

T

M

M

A

A

W

W

L

I

M

L

D

D

E

E

Y

Y

S

S

K

K

A

F

K

H

G

G

T

Y

T

S

V

-

N

P

A

A

V

V

T

T

G

G

K

K

P

P

L

I

V

D

L

L

G

G

S

S

L

L

G

G

R

R

T

D

E

A

A

A

T
|
T

G

G

R

R

G

G

V

V

M

M

V

F

S

G

A

T

L

E

A

A

A

L

M

L

E

N

K

E

L

H

K

G

I

K

N

T

P

I

F

S

Q

G

A

Q

T

R

C

F

A

V

V

I

Q

Q

G

G

F
|
F

G
|
G

N
|
N

V
|
V

G

G

S

S

W

W

A

A

S

A

A

K

L

L

L

I

E

S

E

E

R

K

G

G

L

G

K

K

V

I

V

V

A

A

V

V

S

S

D
|
D

I
|
I

S

T

G

G

A

A

Y

I

Y

K

N

N

A

K

N

D

G

G

I

I

N

D

I

I

Q

P

K

A

A

L

I

L

A

K

Y

H

R

T

D

K

G

E

N

H

K

R

G

G

T

-

L

V

E

K

G

G

F

F

D

D

G

G

A

A

E

D

K

P

L

I

S

-

D

D

P

P

M

N

E

S

L

I

L

L

E

V

L

E

K

D

V

C

D

D

V

I

L

L

V

V

P

P

A
|
A

V

L

E

G

D

G

V

V

I

I

T

N

K

R

A

E

N

N

V

A

D

N

K

E

I

I

N

K

A

A

R

K

L

F

I

I

V

I

E

E

G

A

A
|
A

N
|
N

G

H

P

P

T

T

S

D

F

P

N

D

A

A

D

D

K

E

I

I

I

L

N

S

D

K

K

K

G

G

I

V

M

V

V

I

V

L

P

P

D

D

I

I

L

Y

A

A

N
|
N

A

S

G

G

V
|
V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

I

L

Q

G

G

Y

F

K

M

W

W

T

E

A

E

E

E

R

K

V

V

N

N

R

D

R

E

S

L

D

K

R

T

I

Y

M

M

K

T

E

R

A

S

F

F

D

K

Q

D

V

L

Y

K

K

E

V

M

S

C

V

K

K

T

H

H

G

S

V

C

P

D

M

L

R

R

I

M

A

G

A

A

Y

F

I

T

V

L

A

G

I

V

D

N

K

R

V

V

A

A

Q

Q

T

A

Y

T

Q

I

F

L

R

R

G

G

binding 2-oxoglutaric acid: K66 (= K78), G68 (= G80), M87 (= M99), K90 (= K102), K102 (= K114), A140 (= A152), V341 (= V356), S344 (= S359)
binding potassium ion: S27 (≠ V39), L28 (= L40), I30 (≠ N42), P31 (= P43), F32 (≠ A44)
binding nicotinamide-adenine-dinucleotide: R70 (= R82), D142 (= D154), M143 (= M155), T185 (= T198), F214 (= F227), G215 (= G228), N216 (= N229), V217 (= V230), D237 (= D250), I238 (= I251), A288 (= A303), A289 (= A304), A311 (= A326), N312 (= N327), N337 (= N352)

3aoeB Crystal structure of hetero-hexameric glutamate dehydrogenase from thermus thermophilus (leu bound form)
48% identity, 96% coverage: 15:423/425 of query aligns to 15:421/424 of 3aoeB

query
sites
3aoeB

P

P

L

W

E

E

S

I

M

F

M

T

E

E

R

Q

F

V

N

D

I

R

A

V

E

P

K

Y

L

L

G

G

L

R

S

L

E

A

E

P

V

L

Y

A

N

E

V

S

L

L

K

K

N

R

P

P

A

K

K

R

Q

V

V

L

I

I

V

V

S

D

L

V

P

P

I

V

T

R

M

L

D

D

N

D

G

G

K

S

I

V

K

A

V

Y

F

F

E

E

G

G

I

Y

R

R

V

V

V

H

H

H

S

N

N

T

I
x
A

L

R

G

G

P

P

A

A

K

K

G

G

I

V

R

R

F

Y

A

H

P

P

D

E

V

V

H

T

I

L

D

S

E

E

V

V

R

M

A

A

L

L

A

A

A

G

W

W

M

M

T

T

W

I

K

K

C

N

A

A

V

A

V

V

D

G

I

L

P

P

Y

Y

G

G

K
|
K

G

G

V

I

R

R

C

V

N

D

P

P

R

R

E

K

M

L

S

S

P

P

G

G

E

E

I

L

E

E

R

R

L

L

V

T

R

R

A

R

Y

Y

T

T

L

S

A

E

M

I

I

G

D

I

V

L

F

L

G

G

P

P

D

D

K

R

D

D

I

I

P

P

A

A

P

P

D
|
D

M

V

G

N

T

T

G

G

P

E

K

R

E

E

M

M

A

A

W

W

L

M

M

M

D

D

E

T

Y

Y

S

S

K

M

A

N

K

V

G

G

T

R

T

T

V

V

N

P

A

G

V

V

T

T

G

G

K

K

P

P

L

I

V

A

L

L

G

G

S

S

L

L

G

G

R

R

T

R

E

D

A

A

T

T

G

G

R

R

G

G

V

V

M

F

V

I

S

T

A

A

L

A

A

A

M

A

E

E

K

K

L

I

K

G

I

L

N

Q

P

V

F

E

Q

G

A

A

T

R

C

V

A

A

V

I

Q

Q

G

G

F

F

G

G

N

N

V

V

G

G

S

N

W

A

A

A

S

A

A

R

L

A

L

F

E

H

E

D

R

H

G

G

L

A

K

R

V

V

A

A

V

V

S

Q

D

D

I

H

S

T

G

G

A

T

Y

V

Y

Y

N

N

A

E

N

A

G

G

I

I

N

D

I

P

Q

Y

K

D

A

L

I

L

A

R

Y

H

R

V

D

Q

G

-

N

E

K

F

G

G

T

G

L

V

E

R

G

G

F

Y

D

P

G

K

A

A

E

E

K

P

L

L

S

P

D

-

P

A

M

A

E

D

L

F

L

W

E

G

L

L

K

P

V

V

D

E

V

F

L

L

V

V

P

P

A

A

V

L

E

E

D

K

V

Q

I

I

T

T

K

E

A

Q

N

N

V

A

D

W

K

R

I

I

N

R

A

A

R

R

L

I

I

V

V

A

E

E

G

G

A

A

N

N

G

G

P

P

T

T

S

T

F

P

N

A

A

A

D

D

K

D

I

I

I

L

N

L

D

E

K

K

G

G

I

V

M

L

V

V

P

P

D

D

I

V

L

I

A

A

N

N

A

A

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

D

R

F

L

N

G

S

Y

Y

K

F

W

W

T

T

A

E

E

E

R

E

V

I

N

N

R

A

R

R

S

L

D

E

R

R

I

V

M

L

K

R

E

N

A

A

F

F

D

E

Q

A

V

V

Y

W

K

Q

V

V

S

A

V

Q

K

E

H

K

G

K

V

I

P

P

M

L

R

R

I

T

A

A

Y

Y

I

V

V

V

A

A

I

A

D

T

K

R

V

V

A

L

Q

E

T

A

Y
x
R

Q

A

F

L

R
|
R

G
|
G

active site: K114 (= K114), D154 (= D154)
binding leucine: A73 (≠ I73), R417 (≠ Y419), R420 (= R422), G421 (= G423)

Sites not aligning to the query:

binding leucine: 423

8owmC Crystal structure of glutamate dehydrogenase 2 from arabidopsis thaliana binding ca, NAD and 2,2-dihydroxyglutarate (see paper)
46% identity, 96% coverage: 14:423/425 of query aligns to 4:410/413 of 8owmC

query
sites
8owmC

N

N

P

A

L

L

E

A

S

A

M

T

M

N

E

R

R

N

F

F

N

R

I

H

A

A

A

S

E

R

K

I

L

L

G

G

L

L

S

D

E

S

E

K

V

I

Y

E

N

R

V
x
S

L

L

K

M

N
x
I

P

P

A

F

K

R

Q

E

V

I

I

K

V

V

S

E

L

C

P

T

I

I

T

P

M

K

D

D

N

D

G

G

K

T

I

L

K

V

V

S

F

Y

E

I

G

G

I

F

R

R

V

V

V

Q

H

H

S

D

N

N

I

A

L

R

G

G

P

P

A

M

K
|
K

G

G

G
|
G

I

I

R

R

F

Y

A

H

P

P

D

E

V

V

H

D

I

P

D

D

E

E

V

V

R

N

A

A

L

L

A

A

A

Q

W

L

M
|
M

T

T

W

W

K
|
K

C

T

A

A

V

V

V

A

D

D

I

I

P

P

Y

Y

G

G

G

A

K
|
K

G

G

V

I

R

G

C

C

N

S

P

P

R

R

E

D

M

L

S

S

P

L

G

S

E

E

I

L

E

E

R

R

L

L

V

T

R

R

A

V

Y

F

T

T

L

Q

A

K

M

I

I

H

D

D

V

L

F

I

G

G

P

I

D

H

K

T

D

D

I

V

P

P

A
|
A

P

P

D
|
D

M
|
M

G

G

T

T

G

N

P

A

K

Q

E

T

M

M

A

A

W

W

L

I

M

L

D

D

E

E

Y

Y

S

S

K

K

A

F

K

H

G

G

T

H

T

S

V

-

N

P

A

A

V

V

T

T

G

G

K

K

P

P

L

I

V

D

L

L

G

G

S

S

L

L

G

G

R
|
R

T

E

E

A

A

A

T
|
T

G

G

R

R

G

G

V

V

M

V

V

F

S

A

A

T

L

E

A

A

A

L

M

L

E

A

K

E

L

Y

K

G

I

K

N

S

P

I

F

Q

Q

G

A

L

T

T

C

F

A

V

V

I

Q

Q

G

G

F
|
F

G
|
G

N
|
N

V
|
V

G

G

S

T

W

W

A

A

S

A

A

K

L

L

L

I

E

H

E

E

R

K

G

G

L

G

K

K

V

V

A

A

V

V

S

S

D
|
D

I
|
I

S

T

G

G

A

A

Y

I

Y

R

N

N

A

P

N

E

G

G

I

I

N

D

I

I

Q

N

K

A

A

L

I

I

A

K

Y

H

R

K

D

D

G

A

N

T

K

-

G

G

T

S

L

L

E

N

G

D

F

F

D

N

G

G

A

G

E

D

K

A

L

M

S

N

D

S

P

D

M

-

E

E

L

L

E

I

L

H

K

E

V

C

D

D

V

V

L

L

V

I

P

P

A
x
C

A
|
A

V

L

E

G

D

G

V

V

I

L

T

N

K

K

A

E

N

N

V

A

D

G

K

D

I

V

N

K

A

A

R

K

L

F

I

I

V

V

E

E

G

A

A
|
A

N
|
N

G

H

P

P

T

T

S

D

F

P

N

D

A

A

D

D

K

E

I

I

I

L

N

S

D

K

K

K

G

G

I

V

M

I

V

I

V

L

P

P

D

D

I

I

L

Y

A

A

N
|
N

A

A

G

G

V
|
V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

N

R

I

L

Q

G

G

Y

F

K

M

W

W

T

E

A

E

E

E

R

K

V

V

N

N

R

L

R

E

S

L

D

Q

R

K

I

Y

M

M

K

T

E

R

A

A

F

F

D

H

Q

N

V

I

Y

K

K

T

V

M

S

C

V

H

K

T

H

H

G

S

V

C

P

N

M

L

R

R

I

M

A

G

A

A

Y

F

I

T

V

L

A

G

I

V

D

N

K

R

V

V

A

A

Q

R

T

A

Y

T

Q

Q

F

L

R

R

G

G

binding calcium ion: S29 (≠ V39), I32 (≠ N42)
binding nicotinamide-adenine-dinucleotide: D144 (= D154), M145 (= M155), R183 (= R194), T187 (= T198), F216 (= F227), G217 (= G228), N218 (= N229), V219 (= V230), D239 (= D250), I240 (= I251), C290 (≠ A303), A291 (= A304), A313 (= A326), N314 (= N327), N339 (= N352)
binding 2,2-bis(oxidanyl)pentanedioic acid: K68 (= K78), G70 (= G80), M89 (= M99), K92 (= K102), K104 (= K114), A142 (= A152), R183 (= R194), N314 (= N327), V343 (= V356), S346 (= S359)

4xgiA Crystal structure of glutamate dehydrogenase from burkholderia thailandensis
46% identity, 97% coverage: 11:423/425 of query aligns to 9:413/416 of 4xgiA

query
sites
4xgiA

D

D

K

D

E

L

N

G

P

P

L

W

E

G

S

N

M

Y

M

L

E

Q

R

Q

F

V

N

D

I

R

A

V

A

A

E

P

K

Y

L

L

G

G

L

S

S

L

E

S

E

R

V

W

Y

I

N

E

V

T

L

L

K

K

N

R

P

P

A

K

K

R

Q

I

V

L

I

I

V

V

S

D

L

V

P

P

I

I

T

E

M

L

D

D

N

N

G

G

K

T

I

V

K

A

V

H

F

F

E

E

G

G

I

Y

R

R

V

V

V

Q

H

H

S

N

N

V

I

S

L

R

G

G

P

P

A

G

K
|
K

G

G

G
|
G

I

V

R
|
R

F

Y

A

H

P

Q

D

D

V

V

H

T

I

L

D

S

E

E

V

V

R

M

A

A

L

L

A

S

A

A

W

W

M
|
M

T

S

W

V

K
|
K

C

N

A

A

V

A

V

V

D

N

I

V

P

P

Y

Y

G

G

G

A

K
|
K

G

G

V

I

R

R

C

V

N

D

P

P

R

R

E

K

M

L

S

S

P

R

G

G

E

E

I

L

E

E

R

R

L

V

V

T

R

R

A

R

Y

Y

T

T

L

S

A

E

M

I

I

G

D

I

V

I

F

I

G

G

P

P

D

N

K

T

D

D

I

I

P

P

A
|
A

P

P

D
|
D

M
x
V

G

N

T

T

G

N

P

E

K

Q

E

I

M

M

A

A

W

W

L

M

M

M

D

D

E

T

Y

Y

S

S

K

M

A

N

K

Q

G

G

T

Q

T

T

V

A

N

T

A

G

V

V

T

T

G

G

K

K

P

P

L

I

V

S

L

L

G

G

S

S

L

L

G

G

R
|
R

T

K

E

E

A

A

T
|
T

G

G

R

R

G

G

V

V

M

F

V

V

S

V

A

G

L

C

A

E

A

A

M

A

E

K

K

K

L

K

K

G

I

V

N

E

P

I

F

E

Q

G

A

A

T

R

C

I

A

A

V

V

Q

Q

G
|
G

F

F

G
|
G

N
|
N

V
|
V

G

G

S

G

W

I

A

A

S

A

A

K

L

L

L

F

E

Q

E

E

R

A

G

G

L

A

K

K

V

V

V

I

A

A

V

V

S

Q

D
|
D

I
x
H

S

T

G

G

A

T

Y

I

Y

H

N

Q

A

P

N

A

G

G

I

V

N

D

I

T

Q

A

K

K

A

L

I

L

A

D

Y

H

R

V

D

-

G

G

N

R

K

T

G

G

T

G

L

V

E

A

G

G

F

F

D

E

G

G

A

A

E

E

K

P

L

M

S

P

D

N

P

D

M

-

E

E

L

F

L

W

E

T

L

V

K

E

V

T

D

E

V

I

L

L

V

I

P

P

A
|
A

V

L

E

E

D

N

V

Q

I

I

T

T

K

E

A

K

N

N

V

A

D

S

K

K

I

I

N

R

A

T

R

K

L

I

I

I

V

V

E

E

G

G

A
|
A

N
|
N

G

G

P

P

T

T

S

T

F

T

N

A

A

A

D

D

K

D

I

I

I

L

N

S

D

A

K

N

G

G

I

V

M

L

V

V

V

I

P

P

D

D

I

V

L

I

A

A

N
|
N

A

A

G

G

V

V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

V

Q

Q

N

-

R

-

L

-

G

-

Y

-

K

-

W

W

T

T

A

E

E

D

R

E

V

I

N

N

R

H

R

R

S

L

D

E

R

R

I

V

M

M

K

R

E

E

A

A

F

F

D

A

Q

G

V

V

Y

W

K

A

V

V

S

A

V

E

K

E

H

H

G

K

V

V

P

S

M

V

R

R

I

T

A

A

Y

F

I

I

V

V

A

A

I

C

D

K

K

R

V

I

A

L

Q

M

T

A

Y

R

Q

E

F

M

R

R

G

G

active site: K112 (= K114), D152 (= D154)
binding 2-oxoglutaric acid: K76 (= K78), G78 (= G80), M97 (= M99), K100 (= K102), K112 (= K114), A150 (= A152), R192 (= R194), S355 (= S359)
binding nicotinamide-adenine-dinucleotide: R80 (= R82), D152 (= D154), V153 (≠ M155), T196 (= T198), G224 (= G226), G226 (= G228), N227 (= N229), V228 (= V230), D248 (= D250), H249 (≠ I251), A299 (= A303), A300 (= A304), A322 (= A326), N323 (= N327), N348 (= N352)

P28997 NAD-specific glutamate dehydrogenase; NAD-GDH; EC 1.4.1.2 from Peptoniphilus asaccharolyticus (Peptostreptococcus asaccharolyticus) (see 2 papers)
43% identity, 98% coverage: 9:425/425 of query aligns to 1:421/421 of P28997

query
sites
P28997

I

M

K

T

D

D

K

T

E

L

N

N

P

P

L

L

E

V

S

A

M

A

M

Q

E

E

R

K

F

V

N

R

I

I

A

A

A

C

E

E

K

K

L

L

G

G

L

C

S

D

E

P

E

A

V

V

Y

Y

N

E

V

L

L

L

K

K

N

E

P

P

A

Q

K

R

Q

V

V

I

I

E

V

I

S

S

L

I

P

P

I

V

T

K

M

M

D

D

N

D

G

G

K

T

I

V

K

K

V

V

F

F

E

K

G

G

I

W

R

R

V

S

V

A

H

H

S

S

N

S

I

A

L

V

G

G

P

P

A

S

K

K

G

G

I

V

R

R

F

F

A

H

P

P

D

N

V

V

H

N

I

M

D

D

E

E

V

V

R

K

A

A

L

L

A

S

A

L

W

W

M

M

T

T

W

F

K

K

C

G

A

G

V

A

V

L

D

G

I

L

P

P

Y

Y

G

G

K

K

G

G

V

I

R

C

C

V

N

D

P

P

R

A

E

E

M

L

S

S

P

E

G

R

E

E

I

L

E

E

R

Q

L

L

V

S

R

R

A

G

Y

W

T

V

L

R

A

G

M

L

I

Y

D

K

V

Y

F

L

G

G

P

D

D

R

K

I

D

D

I

I

P

P

A

A

P

P

D

D

M

V

G

N

T

T

G

N

P

G

K

Q

E

I

M

M

A

S

W

W

L

F

M

V

D

D

E

E

Y

Y

S

V

K

K

A

L

K

N

G

G

T

E

T

R

V

M

N

D

-

I

A

G

V

T

V

F

T

T

G

G

K

K

P

P

L

V

V

A

L

F

G

G

S

S

L

E

G

G

R

R

T

N

E

E

A

A

T

T

G

G

R

F

G

G

V

V

M

A

V

V

S

V

A

V

L

R

A

E

A

S

M

A

E

K

K

R

L

F

K

G

I

I

N

K

P

M

F

E

Q

D

A

A

T

K

C

I

A

A

V

V

Q

Q

G

G

F

F

G

G

N

N

V

V

G

G

S

T

W

F

A

T

S

V

A

K

L

N

L

I

E

E

E

R

R

Q

G

G

L

G

K

K

V

V

V

C

A

A

V

I

S

A

D
x
E

I
x
W

-
x
D

-

R

-

N

-

E

-

G

S

N

G

Y

A

A

Y

L

Y

Y

N

N

A

E

N

N

G

G

I

I

N

D

I

F

Q

K

K

E

A

L

I

L

A

A

Y

Y

R

K

D

E

G

A

N

N

K

K

G

-

T

T

L

L

E

I

G

G

F

F

D

P

G

G

A

A

E

E

K

R

L

I

S

T

D

D

P

E

M

-

E

E

L

F

L

W

E

T

L

K

K

E

V

Y

D

D

V

I

L

I

V

V

P

P

A

A

V

L

E

E

D

N

V

V

I

I

T

T

K

G

A

E

N

R

V

A

D

K

K

T

I

I

N

N

A

A

R

K

L

L

I

V

V

C

E

E

G

A

A

A

N

N

G

G

P

P

T

T

S

T

F

P

N

E

A

G

D

D

K

K

I

V

I

L

N

T

D

E

K

R

G

G

I

I

M

N

V

L

V

T

P

P

D

D

I

I

L

L

A

T

N

N

A

S

G

G

V

V

T

L

V

V

S

S

Y

Y

F

Y

E

E

W

W

V

V

Q

Q

N

N

R

Q

L

Y

G

G

Y

Y

K

Y

W

W

T

T

A

E

E

A

R

E

V

V

N

E

R

E

R

K

S

Q

D

E

R

A

I

D

M

M

K

M

E

K

A

A

F

I

D

K

Q

G

V

V

Y

F

K

A

V

V

S

A

V

D

K

E

H

Y

G

N

V

V

P

T

M

L

R

R

I

E

A

A

A

V

Y

Y

I

M

V

Y

A

A

I

I

D

K

K

S

V

I

A

D

Q

V

T

A

Y

M

Q

K

F

L

R

R

G

G

G

W

F

Y

E243 (≠ D250) Important for nucleotide recognition; mutation to D: Shows a 9-fold relaxation of the strong discrimination against NADPH due to the decrease of binding affinity for NADH and the increase for NADPH.; mutation to K: Severely crippled in its ability to bind to NADH. Decrease of binding affinity for NADH and increase for NADPH.; mutation to R: Decrease of binding affinity for NADH and increase for NADPH.
W244 (≠ I251) mutation to S: Decrease of binding affinity for NADH and increase for NADPH.
D245 (vs. gap) mutation to K: Decrease of binding affinity for NADH and increase for NADPH.

1v9lA L-glutamate dehydrogenase from pyrobaculum islandicum complexed with NAD (see paper)
43% identity, 96% coverage: 16:423/425 of query aligns to 4:416/418 of 1v9lA

query
sites
1v9lA

L

L

E

E

S

Y

M

V

M

L

E

N

R

Y

F

V

N

K

I

K

A

G

A

V

E

E

K

L

L

G

G

G

L

F

S

P

E

E

E

D

V

F

Y

Y

N

K

V

I

L

L

K

S

N

R

P

P

A

R

K

R

Q

V

V

L

I

I

V

V

S

N

L

I

P

P

I

V

T

R

M

L

D

D

N

G

G

G

K

G

I

F

K

E

V

V

F

F

E

E

G

G

I

Y

R

R

V

V

V

Q

H

H

S

C

N

D

I

V

L

L

G

G

P

P

A

Y

K

K

G

G

I

V

R

R

F

F

A

H

P

P

D

E

V

V

H

T

I

L

D

A

E

D

V

D

R

V

A

A

L

L

A

A

A

I

W

L

M

M

T

T

W

L

K

K

C

N

A

S

V

L

V

A

D

G

I

L

P

P

Y

Y

G

G

G

A

K
|
K

G

G

G

A

V

V

R

R

C

V

N

D

P

P

R

K

E

K

M

L

S

S

P

Q

G

R

E

E

I

L

E

E

R

E

L

L

V

S

R

R

A

G

Y

Y

T

A

L

R

A

A

M

I

I

A

D

P

V

L

F

I

G

G

P

D

D

V

K

V

D

D

I

I

P

P

A

A

P

P

D
|
D

M

V

G

G

T

T

G

N

P

A

K

Q

E

I

M

M

A

A

W

W

L

M

M

V

D

D

E

E

Y

Y

S

S

K

K

A

I

K

K

G

G

T

Y

T

N

V

V

N

P

A

G

V

V

V

F

T

T

G

S

K

K

P

P

L

P

V

E

L

L

G

W

G

G

S

N

L

P

G

V

R

R

T

E

E

Y

A

A

T
|
T

G

G

R

F

G

G

V

V

M

A

V

V

S

A

A

T

L

R

A

E

A

M

M

A

E

K

K

K

L

L

K

W

I

G

N

G

P

I

F

E

Q

G

A

K

T

T

C

V

A

A

V

I

Q
|
Q

G

G

F

M

G
|
G

N
|
N

V
|
V

G

G

S

R

W

W

A

T

S

A

A

Y

L

W

L

L

E

E

E

K

R

M

G

G

L

A

K

K

V

V

V

I

A

A

V

V

S

S

D
|
D

I
|
I

S

N

G

G

A

V

Y

A

Y

Y

N

R

A

K

N

E

G

G

I

L

N

N

I

V

Q

E

K

-

A

L

I

I

A

Q

Y

K

R

N

D

K

G

G

N

L

K

T

G

G

-

P

-

A

T

L

L

V

E

E

G

L

F

F

-

T

-

K

D

D

G

N

A

A

E

E

K

F

L

V

S

K

D

N

P

P

M

D

E

A

L

I

L

F

E

K

L

L

K

D

V

V

D

D

V

I

L

F

V

V

P

P

A

A

A
|
A

V
x
I

E

E

D

N

V

V

I

I

T

R

K

G

A

D

N

N

V

A

D

G

K

L

I

V

N

K

A

A

R

R

L

L

I

V

V

V

E

E

G

G

A
|
A

N
|
N

G

G

P

P

T

T

S

T

F

P

N

E

A

A

D

E

K

R

I

I

I

L

N

Y

D

E

K

R

G

G

I

V

M

V

V

V

P

P

D

D

I

I

L

L

A

A

N
|
N

A

A

G

G

V

V

T

I

V

M

S

S

Y

Y

F

L

E

E

W

W

V

V

Q

E

N

N

R

L

L

Q

G

W

Y

Y

K

I

W

W

T

D

A

E

E

E

R

E

V

T

N

R

R

K

R

R

S

L

D

E

R

N

I

I

M

M

K

V

E

N

A

N

F

V

D

E

Q

R

V

V

Y

Y

K

K

V

R

S

W

V

Q

K

R

H

E

-

K

G

G

V

W

P

T

M

M

R

R

I

D

A

A

Y

I

I

V

V

T

A

A

I

L

D

E

K

R

V

I

A

Y

Q

N

T

A

Y

M

Q

K

F

I

R

R

G

G

active site: K102 (= K114), D142 (= D154)
binding nicotinamide-adenine-dinucleotide: T186 (= T198), Q213 (= Q225), G216 (= G228), N217 (= N229), V218 (= V230), D238 (= D250), I239 (= I251), A296 (= A304), I297 (≠ V305), A318 (= A326), N319 (= N327), N344 (= N352)

P80053 Glutamate dehydrogenase 2; GDH-2; EC 1.4.1.3 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
43% identity, 93% coverage: 28:423/425 of query aligns to 23:418/420 of P80053

query
sites
P80053

E

E

K

L

L

L

G

G

L

L

S

D

E

N

E

E

V

T

Y

L

N

E

V

T

L

L

K

S

N

Q

P

P

A

E

K

R

Q

I

V

I

I

Q

V

V

S

K

L

I

P

Q

I

I

T

R

M

G

D

S

N

D

G

G

K

K

I

L

K

K

V

T

F

F

E

M

G

G

I

W

R

R

V

S

V

Q

H

H

S

N

N

S

I

A

L

L

G

G

P

P

A

Y

K

K

G

G

I

V

R

R

F

Y

A

H

P

P

D

N

V

V

H

T

I

Q

D

D

E

E

V

V

R

E

A

A

L

L

A

S

A

M

W

I

M

M

T

T

W

W

K

K

C

N

A

S

V

L

D

L

I

L

P

P

Y

Y

G

G

K

K

G

G

V

V

R

R

C

V

N

D

P

P

R

K

E

K

M

L

S

T

P

R

G

E

E

E

I

L

E

E

R

Q

L

L

V

S

R

R

A

K

Y

Y

T

I

L

Q

A

A

M

I

I

Y

D

K

V

Y

F

L

G

G

P

S

D

E

K

L

D

D

I

I

P

P

A

A

P

P

D

D

M

V

G

N

T

T

G

D

P

S

K

Q

E

T

M

M

A

A

W

W

L

F

M

L

D

D

E

E

Y

Y

S

I

K

K

A

I

K

T

G

G

T

K

T

V

V

D

N

F

A

A

V

V

V

F

T

T

G

G

K

K

P

P

L

V

V

E

L

L

G

G

S

I

L

G

G

V

R

R

T

L

E

Y

A

S

T

T

G

G

R

L

G

G

V

V

M

A

V

T

S

I

A

A

L

K

A

E

A

A

M

A

E

N

K

K

L

F

K

I

I

G

N

G

P

V

F

E

Q

E

A

A

T

R

C

V

A

I

V

I

Q

Q

G

G

F

F

G

G

N

N

V

V

G

G

S

Y

W

Y

A

A

S

G

A

K

L

F

L

L

E

S

E

E

R

M

G

G

L

A

K

K

V

I

V

V

A

G

V

V

S

S

D

D

I

S

S

K

G

G

A

G

Y

V

Y

I

N

N

A

E

N
x
K

G

G

I

I

N

D

I

V

Q

G

K
|
K

A

A

I

I

A

E

Y

I

R

K

D

E

G

-

N

K

K

T

G

G

T

S

L

V

E

I

G

N

F

Y

D

P

G

E

A

G

E

R

K

K

L

V

S

T

D

N

P

E

M

-

E

E

L

L

E

I

L

S

K

D

V

C

D

D

V

I

L

L

V

I

P

P

A

A

V

L

E

E

D

N

V

V

I

I

T

N

K

K

A

F

N

N

V

A

D

P

K

K

I

V

N

K

A

A

R

K

L

L

I

I

V

V

E

E

G

G

A

A

N

N

G

G

P

P

T

L

S

T

F

A

N

D

A

A

D

D

K

E

I

I

I

M

N

R

D

Q

K

R

G

G

I

I

M

A

V

V

P

P

D

D

I

I

L

L

A

A

N

N

A

A

G

G

V

V

T

V

V

G

S

S

Y

Y

F

V

E

E

W

W

V

A

Q

N

N

N

R

K

L

M

G

G

Y

E

K

I

W

I

T

S

A

D

E

E

R

E

V

A

N

K

R
x
K

R

L

S

I

D

V

R

D

I

R

M

M

K

N

E

N

A

A

F

F

D

N

Q

T

V

L

Y

Y

K

D

V

Y

S

H

V

Q

K
|
K

-
x
K

-

L

H

E

G

D

V

H

P

D

M

L

R

R

I

T

A

A

Y

M

I

A

V

L

A

A

I

V

D

D

K

R

V

V

A

V

Q

R

T

A

Y

M

Q

K

F

A

R

R

G

G

K254 (≠ N259) modified: N6-methyllysine
K260 (= K265) modified: N6-methyllysine
K372 (≠ R379) modified: N6-methyllysine
K391 (= K398) modified: N6-methyllysine
K392 (vs. gap) modified: N6-methyllysine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylmethionine

6dhlA Bovine glutamate dehydrogenase complexed with epicatechin-3-gallate (ecg) (see paper)
39% identity, 89% coverage: 43:420/425 of query aligns to 49:489/496 of 6dhlA

query
sites
6dhlA

P

P

A

C

K

N

Q

H

V

V

I

L

-

S

V

L

S

S

L

F

P

P

I

I

T

R

M

R

D

D

N

D

G

G

K

S

I

W

K

E

V

V

F

I

E

E

G

G

I

Y

R

R

V

A

V

Q

H

H

S

S

N

Q

I
x
H

L
x
R

G

T

P

P

A

C

K

K

G

G

I

I

R

R

F

Y

A

S

P

T

D

D

V

V

H

S

I

V

D

D

E

E

V

V

R

K

A

A

L

L

A

A

A

S

W

L

M

M

T

T

W

Y

K

K

C
|
C

A

A

V

V

D
|
D

I
x
V

P

P

Y

F

G

G

G

A

K

K

G

A

G

G

V

V

R

K

C

I

N

N

P

P

R

K

E

N

M

Y

S

T

P

D

G

N

E

E

I

L

E

E

R

K

L

I

V

T

R

R

A

R

Y

F

T

T

-

M

-

E

L

L

A

A

M

K

I

K

D

G

V

F

F

I

G

G

P

P

D

G

K

V

D

D

I

V

P

P

A

A

P

P

D

D

M

M

G

S

T

T

G

G

P

E

K

R

E

E

M

M

A

S

W

W

L

I

M

A

D

D

E

T

Y

Y

S

A

K

S

A

T

K

I

G

G

T

H

-

Y

-

D

-

I

T

N

V

A

N

H

A

A

V

C

V

V

T

T

G

G

K

K

P

P

L

I

V

S

L

Q

G

G

S

I

L

H

G

G

R

R

T

I

E

S

A

A

T

T

G

G

R

R

G

G

V

V

-

F

-

H

-

G

-

I

-

E

-

N

M

F

V

I

S

N

A

E

L

A

A

S

A

Y

M

M

E

S

K

I

L

L

K

G

I

M

N

T

P

P

-

G

-

F

F

G

Q

D

A

K

T

T

C

F

A

A

V

V

Q

Q

G

G

F

F

G

G

N

N

V

V

G

G

S

L

W

H

A

S

S

M

A

R

L

Y

L

L

E

H

E

R

R

F

G

G

L

A

K

K

V

C

V

V

A

A

V

V

S

G

D

E

I

S

S

D

G

G

A

S

Y

I

Y

W

N

N

A

P

N

D

G

G

I

I

N

D

I

P

Q

K

K

E

A

L

I

E

A

D

Y

F

R

K

D

L

G

-

N

Q

K

H

G

G

T

T

L

I

E

L

G

G

F

F

D

P

G

K

A

A

E

K

K

I

L

Y

S

E

D

G

P

-

M

-

E

S

L

I

L

L

E

E

L

V

K

D

V

C

D

D

V

I

L

L

V

I

P

P

A

A

V

S

E

E

D

K

V

Q

I

L

T

T

K

K

A

S

N

N

V

A

D

P

K

R

I

V

N

K

A

A

R

K

L

I

I

I

V

A

E

E

G

G

A

A

N

N

G

G

P

P

T

T

S

T

F

P

N

E

A

A

D

D

K

K

I

I

I

F

N

L

D

E

K

R

G

N

I

I

M

M

V

V

V

I

P

P

D

D

I

L

L

Y

A

L

N

N

A

A

G

G

V

V

T

T

V

V

S

S

Y

Y

F

F

E

E

W

W

V

L

Q
x
K

N

N

-

L

-

N

-

H

-

V

-
x
S

-

Y

-

G

R
|
R

L

L

G

T

Y

F

K

K

W

Y

-

E

-

R

-

D

-

S

-

N

-

Y

-

H

-

L

-

M

-

S

T

V

A

Q

E

E

R

S

V

L

N

E

R

R

R

K

-

F

-

G

-

K

-

H

-

G

-

T

-

I

-

P

-

I

-

V

-

P

-

T

-

A

-

E

-

F

S

Q

D

D

R

R

I

I

-

S

-

G

-

A

-

S

-

E

-

K

-

D

-

I

-

V

-

H

-

S

-

G

-

L

-

A

-

Y

-

T

M

M

K

E

E
x
R

A

S

F

A

D

R

Q

Q

V

I

Y

M

K

R

V

T

S

A

V

M

K

K

H

Y

-

N

-

L

G

G

V

L

P

D

M

L

R

R

I

T

A

A

Y

Y

I

V

V

N

A

A

I

I

D

E

K
|
K

V

V

A

F

Q

R

T

V

Y

Y

Q

N

binding (2R,3S)-2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3,4-dihydro-2H-chromen-3-yl 3,4,5-trihydroxybenzoate: H80 (≠ I73), R81 (≠ L74), C110 (= C103), D114 (= D107), V115 (≠ I108), K382 (≠ Q365), S388 (vs. gap), R391 (= R367), R454 (≠ E387), K483 (= K414)

3eteA Crystal structure of bovine glutamate dehydrogenase complexed with hexachlorophene (see paper)
39% identity, 89% coverage: 43:420/425 of query aligns to 51:491/495 of 3eteA

query
sites
3eteA

P

P

A

C

K

N

Q

H

V

V

I

L

-

S

V

L

S

S

L

F

P

P

I

I

T

R

M

R

D

D

N

D

G

G

K

S

I

W

K

E

V

V

F

I

E

E

G

G

I

Y

R

R

V

A

V

Q

H

H

S

S

N

Q

I

H

L

R

G

T

P

P

A

C

K
|
K

G

G

G
|
G

I

I

R
|
R

F

Y

A

S

P

T

D

D

V

V

H

S

I

V

D

D

E

E

V

V

R

K

A

A

L

L

A

A

A

S

W

L

M
|
M

T

T

W

Y

K
|
K

C

C

A

A

V

V

D

D

I

V

P

P

Y

F

G

G

G

A

K
|
K

G

A

G

G

V

V

R

K

C

I

N

N

P

P

R
x
K

E

N

M

Y

S

T

P

D

G

N

E

E

I

L

E

E

R

K

L

I

V

T

R

R

A

R

Y

F

T

T

-

M

-

E

L

L

A

A

M

K

I

K

D

G

V

F

F

I

G

G

P

P

D

G

K

V

D

D

I

V

P

P

A
|
A

P

P

D
|
D

M
|
M

G

S

T

T

G

G

P

E

K

R

E

E

M

M

A

S

W

W

L

I

M

A

D

D

E

T

Y

Y

S

A

K

S

A
x
T

K
x
I

G

G

T

H

-
x
Y

-

D

-

I

T

N

V

A

N

H

A

A

V

C

V

V

T

T

G

G

K

K

P

P

L

I

V

S

L

Q

G

G

S

I

L

H

G

G

R
|
R

T
x
I

E
x
S

A

A

T

T

G

G

R
|
R

G

G

V

V

-

F

-

H

-

G

-

I

-

E

-

N

M

F

V

I

S

N

A

E

L

A

A

S

A

Y

M

M

E

S

K

I

L

L

K

G

I

M

N

T

P

P

-

G

-

F

F

G

Q

D

A

K

T

T

C

F

A

A

V

V

Q

Q

G

G

F
|
F

G
|
G

N

N

V
|
V

G

G

S

L

W
x
H

A

S

S

M

A
x
R

L
x
Y

L

L

E

H

E

R

R

F

G

G

L

A

K

K

V

C

V

V

A

A

V

V

S

G

D
x
E

I
x
S

S

D

G

G

A

S

Y

I

Y

W

N

N

A

P

N

D

G

G

I

I

N

D

I

P

Q

K

K

E

A

L

I

E

A

D

Y

F

R

K

D

L

G

-

N

Q

K

H

G

G

T

T

L

I

E

L

G

G

F

F

D

P

G

K

A

A

E

K

K

I

L

Y

S

E

D

G

P

-

M

-

E

S

L

I

L

L

E

E

L

V

K

D

V

C

D

D

V

I

L

L

V

I

P

P

A

A

A
|
A

V

S

E

E

D

K

V

Q

I

L

T

T

K

K

A

S

N

N

V

A

D

P

K

R

I

V

N

K

A

A

R

K

L

I

I

I

V

A

E

E

G

G

A
|
A

N
|
N

G

G

P

P

T

T

S

T

F

P

N

E

A

A

D

D

K

K

I

I

I

F

N

L

D

E

K

R

G

N

I

I

M

M

V

V

V

I

P

P

D

D

I

L

L

Y

A

L

N
|
N

A

A

G

G

V
|
V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

L

Q

K

N

N

-

L

-

N

-

H

-

V

-

S

-

Y

-

G

R

R

L

L

G

T

Y

F

K

K

W

Y

-

E

-

R

-

D

-

S

-

N

-

Y

-

H

-

L

-

M

-

S

T

V

A

Q

E

E

R

S

V

L

N

E

R

R

R

K

-

F

-

G

-

K

-

H

-

G

-

T

-

I

-

P

-

I

-

V

-

P

-

T

-

A

-

E

-

F

S

Q

D

D

R

R

I

I

-

S

-

G

-

A

-

S

-

E

-
x
K

-

D

-

I

-

V

-
x
H

-

S

-

G

-

L

-

A

-

Y

-

T

M

M

K

E

E

R

A

S

F

A

D

R

Q

Q

V

I

Y

M

K

R

V

T

S

A

V

M

K

K

H

Y

-

N

-

L

G

G

V

L

P

D

M

L

R

R

I

T

A

A

Y

Y

I

V

V

N

A

A

I

I

D

E

K

K

V

V

A

F

Q

R

T

V

Y

Y

Q

N

active site: K123 (= K114), D165 (= D154)
binding glutamic acid: K87 (= K78), G89 (= G80), M108 (= M99), K111 (= K102), K123 (= K114), A163 (= A152), R208 (= R194), V375 (= V356), S378 (= S359)
binding guanosine-5'-triphosphate: I209 (≠ T195), S210 (≠ E196), R214 (= R200), H255 (≠ W233), R258 (≠ A236), Y259 (≠ L237), K443 (vs. gap), H447 (vs. gap)
binding 2,2'-methanediylbis(3,4,6-trichlorophenol): T183 (≠ A172), I184 (≠ K173), Y187 (vs. gap)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R91 (= R82), K131 (≠ R122), D165 (= D154), M166 (= M155), R208 (= R194), F249 (= F227), G250 (= G228), V252 (= V230), E272 (≠ D250), S273 (≠ I251), A323 (= A304), A345 (= A326), N346 (= N327), N371 (= N352)

6dhdA Bovine glutamate dehydrogenase complexed with nadh, gtp, glutamate (see paper)
39% identity, 89% coverage: 43:420/425 of query aligns to 54:494/501 of 6dhdA

query
sites
6dhdA

P

P

A

C

K

N

Q

H

V

V

I

L

-

S

V

L

S

S

L

F

P

P

I

I

T

R

M

R

D

D

N

D

G

G

K

S

I

W

K

E

V

V

F

I

E

E

G

G

I

Y

R

R

V

A

V

Q

H

H

S

S

N

Q

I
x
H

L
x
R

G

T

P

P

A

C

K
|
K

G

G

I

I

R
|
R

F

Y

A

S

P

T

D

D

V

V

H

S

I

V

D

D

E

E

V

V

R

K

A

A

L

L

A

A

A

S

W

L

M
|
M

T

T

W

Y

K
|
K

C

C

A
|
A

V

V

D
|
D

I
x
V

P

P

Y

F

G

G

G

A

K
|
K

G

A

G

G

V

V

R

K

C

I

N

N

P

P

R

K

E

N

M

Y

S

T

P

D

G

N

E

E

I

L

E

E

R

K

L

I

V

T

R

R

A

R

Y

F

T

T

-

M

-

E

L

L

A

A

M

K

I

K

D

G

V

F

F

I

G

G

P

P

D

G

K

V

D

D

I

V

P

P

A
|
A

P

P

D
|
D

M
|
M

G

S

T

T

G

G

P

E

K

R

E

E

M

M

A

S

W

W

L

I

M

A

D

D

E

T

Y

Y

S

A

K

S

A

T

K

I

G

G

T

H

-

Y

-

D

-

I

T

N

V

A

N
x
H

A

A

V

C

V

V

T

T

G

G

K

K

P

P

L

I

V

S

L
x
Q

G
|
G

G

G

S

I

L
x
H

G

G

R

R

T

I

E
x
S

A

A

T
|
T

G

G

R
|
R

G

G

V

V

-

F

-

H

-

G

-

I

-

E

-

N

M

F

V

I

S

N

A

E

L

A

A

S

A

Y

M

M

E

S

K

I

L

L

K

G

I

M

N

T

P

P

-

G

-

F

F

G

Q

D

A

K

T

T

C

F

A

A

V

V

Q
|
Q

G

G

F
|
F

G
|
G

N
|
N

V
|
V

G

G

S

L

W
x
H

A

S

S

M

A
x
R

L
x
Y

L

L

E

H

E
x
R

R

F

G

G

L

A

K

K

V

C

V

V

A

A

V

V

S

G

D
x
E

I
x
S

S

D

G

G

A

S

Y

I

Y

W

N

N

A

P

N

D

G

G

I

I

N

D

I

P

Q

K

K

E

A

L

I
x
E

A

D

Y

F

R

K

D

L

G

-

N

Q

K

H

G

G

T

T

L

I

E

L

G

G

F

F

D

P

G

K

A

A

E

K

K

I

L

Y

S

E

D

G

P

-

M

-

E

S

L

I

L

L

E

E

L

V

K

D

V

C

D

D

V

I

L

L

V

I

P

P

A

A

A
|
A

V

S

E

E

D

K

V

Q

I

L

T

T

K

K

A

S

N

N

V

A

D

P

K

R

I

V

N

K

A

A

R

K

L

I

I

I

V

A

E

E

G

G

A
|
A

N
|
N

G

G

P

P

T

T

S

T

F

P

N

E

A

A

D

D

K

K

I

I

I

F

N

L

D

E

K

R

G

N

I

I

M

M

V

V

V

I

P

P

D

D

I

L

L

Y

A

L

N
|
N

A

A

G

G

V
|
V

T

T

V

V

S
|
S

Y

Y

F

F

E

E

W

W

V

L

Q
x
K

N
|
N

-

L

-

N

-
x
H

-

V

-
x
S

-

Y

-

G

R

R

L

L

G

T

Y

F

K

K

W

Y

-

E

-

R

-

D

-

S

-

N

-

Y

-

H

-

L

-

M

-

S

T

V

A

Q

E

E

R

S

V

L

N

E

R

R

R

K

-

F

-

G

-

K

-

H

-

G

-

T

-

I

-

P

-

I

-

V

-

P

-

T

-

A

-

E

-

F

S

Q

D

D

R

R

I

I

-

S

-

G

-

A

-

S

-

E

-

K

-

D

-

I

-

V

-
x
H

-

S

-

G

-

L

-

A

-

Y

-

T

M

M

K

E

E

R

A

S

F

A

D

R

Q

Q

V

I

Y

M

K

R

V

T

S

A

V

M

K

K

H

Y

-

N

-

L

G

G

V

L

P

D

M

L

R

R

I

T

A

A

Y

Y

I

V

V

N

A

A

I

I

D

E

K
|
K

V

V

A

F

Q
x
R

T

V

Y

Y

Q

N

active site: K126 (= K114), D168 (= D154)
binding glutamic acid: K90 (= K78), M111 (= M99), K114 (= K102), K126 (= K114), A166 (= A152), V378 (= V356), S381 (= S359)
binding guanosine-5'-triphosphate: H209 (≠ L192), S213 (≠ E196), R217 (= R200), H258 (≠ W233), R261 (≠ A236), Y262 (≠ L237), R265 (≠ E240), E292 (≠ I267), H450 (vs. gap)
binding 1,4-dihydronicotinamide adenine dinucleotide: H85 (≠ I73), R86 (≠ L74), R94 (= R82), A116 (= A104), D119 (= D107), V120 (≠ I108), D168 (= D154), M169 (= M155), H195 (≠ N178), Q205 (≠ L188), G206 (= G189), T215 (= T198), Q250 (= Q225), F252 (= F227), G253 (= G228), N254 (= N229), V255 (= V230), E275 (≠ D250), S276 (≠ I251), A326 (= A304), A348 (= A326), N349 (= N327), N374 (= N352), K387 (≠ Q365), N388 (= N366), H391 (vs. gap), S393 (vs. gap), K488 (= K414), R491 (≠ Q417)

3etgA Glutamate dehydrogenase complexed with gw5074 (see paper)
39% identity, 89% coverage: 43:420/425 of query aligns to 54:494/501 of 3etgA

query
sites
3etgA

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active site: K126 (= K114), D168 (= D154)
binding glutamic acid: K90 (= K78), M111 (= M99), K114 (= K102), A166 (= A152), V378 (= V356), S381 (= S359)
binding guanosine-5'-triphosphate: H209 (≠ L192), G210 (= G193), S213 (≠ E196), R217 (= R200), R261 (≠ A236), R265 (≠ E240), E292 (≠ I267)
binding (3E)-3-[(3,5-dibromo-4-hydroxyphenyl)methylidene]-5-iodo-1,3-dihydro-2H-indol-2-one: R146 (= R134), R147 (≠ A135), M150 (vs. gap)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: D168 (= D154), M169 (= M155), R211 (= R194), T215 (= T198), Q250 (= Q225), G253 (= G228), V255 (= V230), E275 (≠ D250), S276 (≠ I251), A326 (= A304), G347 (= G325), A348 (= A326), N349 (= N327), N374 (= N352)

3etdA Structure of glutamate dehydrogenase complexed with bithionol (see paper)
39% identity, 89% coverage: 43:420/425 of query aligns to 54:494/501 of 3etdA

query
sites
3etdA

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S

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active site: K126 (= K114), D168 (= D154)
binding 2,2'-sulfanediylbis(4,6-dichlorophenol): R146 (= R134), R146 (= R134), R147 (≠ A135), D181 (= D167), S185 (≠ K171)
binding glutamic acid: K90 (= K78), G92 (= G80), M111 (= M99), K114 (= K102), K126 (= K114), A166 (= A152), S381 (= S359)
binding guanosine-5'-triphosphate: H209 (≠ L192), G210 (= G193), I212 (≠ T195), R217 (= R200), R261 (≠ A236), R265 (≠ E240), E292 (≠ I267), H450 (vs. gap)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R94 (= R82), D168 (= D154), M169 (= M155), R211 (= R194), T215 (= T198), Q250 (= Q225), F252 (= F227), G253 (= G228), V255 (= V230), E275 (≠ D250), S276 (≠ I251), A326 (= A304), A348 (= A326), N349 (= N327), N374 (= N352)

Query Sequence

>Echvi_1535 FitnessBrowser__Cola:Echvi_1535
MAYIEPAPIKDKENPLESMMERFNIAAEKLGLSEEVYNVLKNPAKQVIVSLPITMDNGKI
KVFEGIRVVHSNILGPAKGGIRFAPDVHIDEVRALAAWMTWKCAVVDIPYGGGKGGVRCN
PREMSPGEIERLVRAYTLAMIDVFGPDKDIPAPDMGTGPKEMAWLMDEYSKAKGTTVNAV
VTGKPLVLGGSLGRTEATGRGVMVSALAAMEKLKINPFQATCAVQGFGNVGSWASALLEE
RGLKVVAVSDISGAYYNANGINIQKAIAYRDGNKGTLEGFDGAEKLSDPMELLELKVDVL
VPAAVEDVITKANVDKINARLIVEGANGPTSFNADKIINDKGIMVVPDILANAGGVTVSY
FEWVQNRLGYKWTAERVNRRSDRIMKEAFDQVYKVSVKHGVPMRIAAYIVAIDKVAQTYQ
FRGGF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory