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Comparing Echvi_1626 FitnessBrowser__Cola:Echvi_1626 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
A0R066 Branched-chain-amino-acid aminotransferase; BCAT; EC 2.6.1.42 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see 2 papers)
41% identity, 94% coverage: 12:345/355 of query aligns to 20:363/368 of A0R066
- R101 (= R92) binding
- Y209 (= Y196) binding
- K299 (≠ E282) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- T314 (= T297) binding
3dtgA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design
41% identity, 94% coverage: 12:345/355 of query aligns to 15:358/363 of 3dtgA
- active site: K199 (= K191)
- binding O-benzylhydroxylamine: F72 (= F68), Y171 (= Y166), Y204 (= Y196)
- binding pyridoxal-5'-phosphate: R96 (= R92), R189 (= R181), K199 (= K191), Y204 (= Y196), E235 (= E227), G238 (≠ T230), L263 (= L251), I266 (≠ V254), T267 (= T255), T309 (= T297)
3dtfA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design (see paper)
41% identity, 94% coverage: 12:345/355 of query aligns to 15:358/363 of 3dtfA
3ht5A Crystal structure of ilve a branched chain amino acid transaminase from mycobacterium tuberculosis (see paper)
40% identity, 90% coverage: 28:345/355 of query aligns to 3:330/335 of 3ht5A
- active site: K171 (= K191)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R68 (= R92), K171 (= K191), Y176 (= Y196), E207 (= E227), G210 (≠ T230), M211 (= M231), N212 (= N232), L235 (= L251), G237 (= G253), I238 (≠ V254), T239 (= T255), T281 (= T297)
5u3fA Structure of mycobacterium tuberculosis ilve, a branched-chain amino acid transaminase, in complex with d-cycloserine derivative (see paper)
40% identity, 90% coverage: 28:345/355 of query aligns to 2:321/326 of 5u3fA
- active site: K162 (= K191)
- binding (5-hydroxy-6-methyl-4-{[(3-oxo-2,3-dihydro-1,2-oxazol-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: G45 (= G70), R67 (= R92), Y110 (= Y134), K162 (= K191), Y167 (= Y196), E198 (= E227), G201 (≠ T230), M202 (= M231), N203 (= N232), L226 (= L251), G228 (= G253), I229 (≠ V254), T230 (= T255), T272 (= T297)
5bwvA X-ray crystal structure at 1.86a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a pyrazolopyrimidinone compound and an internal aldimine linked plp cofactor. (see paper)
39% identity, 80% coverage: 23:307/355 of query aligns to 24:313/355 of 5bwvA
- active site: K192 (= K191)
- binding 2-[(4-chlorobenzyl)amino]-7-oxo-5-propyl-4,7-dihydropyrazolo[1,5-a]pyrimidine-3-carbonitrile: R137 (= R136), Y167 (= Y166), V172 (≠ L171), Q214 (= Q213), V228 (≠ S228), T230 (= T230), M231 (= M231), G302 (= G296), A304 (= A298), C305 (≠ A299), C308 (≠ A302)
- binding pyridoxal-5'-phosphate: R93 (= R92), K192 (= K191), Y197 (= Y196), E227 (= E227), T230 (= T230), N232 (= N232), L256 (= L251), G258 (= G253), V259 (= V254), V260 (≠ T255), G302 (= G296), T303 (= T297)
5bwtA X-ray crystal structure at 2.20a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a pyrazolopyrimidinone fragment and an internal aldimine linked plp. (see paper)
39% identity, 80% coverage: 23:307/355 of query aligns to 28:318/360 of 5bwtA
- active site: K197 (= K191)
- binding 5-ethyl-2-methyl-7-oxo-4,7-dihydropyrazolo[1,5-a]pyrimidine-3-carbonitrile: Y171 (= Y166), T235 (= T230), M236 (= M231), A309 (= A298), C310 (≠ A299)
- binding pyridoxal-5'-phosphate: R97 (= R92), K197 (= K191), Y202 (= Y196), E232 (= E227), T235 (= T230), M236 (= M231), N237 (= N232), L261 (= L251), G263 (= G253), V264 (= V254), V265 (≠ T255), G307 (= G296), T308 (= T297)
5i5xA X-ray crystal structure at 1.65a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a thiazole compound and pmp cofactor. (see paper)
39% identity, 80% coverage: 23:307/355 of query aligns to 25:316/358 of 5i5xA
- active site: K195 (= K191)
- binding 5-methyl-4-oxo-N-(1,3,4-thiadiazol-2-yl)-3,4-dihydrothieno[2,3-d]pyrimidine-6-carboxamide: F25 (= F23), F70 (= F68), K74 (= K72), Y136 (= Y134), V165 (≠ M163), Y168 (= Y166), A307 (= A298)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R94 (= R92), R185 (= R181), K195 (= K191), Y200 (= Y196), E230 (= E227), T233 (= T230), M234 (= M231), N235 (= N232), L259 (= L251), G261 (= G253), V262 (= V254), V263 (≠ T255), T306 (= T297)
5i60A X-ray crystal structure at 2.12a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a biarl amide compound and an internal aldimine linked plp cofactor. (see paper)
39% identity, 80% coverage: 23:307/355 of query aligns to 24:317/359 of 5i60A
- active site: K196 (= K191)
- binding (2-{[4-(1H-pyrazol-3-yl)phenyl]carbamoyl}phenyl)acetic acid: F24 (= F23), Y135 (= Y134), G165 (≠ A164), Y167 (= Y166), T234 (= T230), G306 (= G296), A308 (= A298)
- binding pyridoxal-5'-phosphate: R93 (= R92), R186 (= R181), K196 (= K191), Y201 (= Y196), E231 (= E227), T234 (= T230), M235 (= M231), L260 (= L251), G262 (= G253), V263 (= V254), V264 (≠ T255), T307 (= T297)
5hneA X-ray crystal structure of human mitochondrial branched chain aminotransferase (bcatm) complexed with a 2-aryl benzimidazole compound and an internal aldimine linked plp cofactor (see paper)
39% identity, 80% coverage: 23:307/355 of query aligns to 24:317/359 of 5hneA
- active site: K196 (= K191)
- binding 1-[(1R,3S)-3-{[(5-bromothiophen-2-yl)carbonyl]amino}cyclohexyl]-N-methyl-2-(pyridin-2-yl)-1H-benzimidazole-5-carboxamide: F24 (= F23), F69 (= F68), K73 (= K72), Y167 (= Y166), Q218 (= Q213), V232 (≠ S228), G233 (= G229), T234 (= T230), M235 (= M231), A308 (= A298), C312 (≠ A302)
- binding pyridoxal-5'-phosphate: R93 (= R92), K196 (= K191), Y201 (= Y196), E231 (= E227), T234 (= T230), N236 (= N232), L260 (= L251), G262 (= G253), V263 (= V254), V264 (≠ T255), G306 (= G296), T307 (= T297)
5i5vA X-ray crystal structure at 1.94a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a thienopyrimidine compound and an internal aldimine linked plp cofactor. (see paper)
39% identity, 80% coverage: 23:307/355 of query aligns to 25:318/360 of 5i5vA
- active site: K197 (= K191)
- binding 3,5-dimethyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-6-carboxylic acid: F25 (= F23), K74 (= K72), Y136 (= Y134), Y168 (= Y166), A309 (= A298)
- binding pyridoxal-5'-phosphate: R94 (= R92), R187 (= R181), K197 (= K191), Y202 (= Y196), E232 (= E227), T235 (= T230), M236 (= M231), L261 (= L251), G263 (= G253), V264 (= V254), V265 (≠ T255), G307 (= G296), T308 (= T297)
5i5sA X-ray crystal structure at 2.06a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a benzisoxazole compound and an internal aldimine linked plp cofactor. (see paper)
39% identity, 80% coverage: 23:307/355 of query aligns to 26:319/361 of 5i5sA
- active site: K198 (= K191)
- binding 2-(1,2-benzoxazol-3-yl)ethanoic acid: F26 (= F23), F71 (= F68), K198 (= K191), T236 (= T230), T309 (= T297), A310 (= A298)
- binding pyridoxal-5'-phosphate: R95 (= R92), R188 (= R181), K198 (= K191), Y203 (= Y196), E233 (= E227), T236 (= T230), M237 (= M231), N238 (= N232), L262 (= L251), G264 (= G253), V265 (= V254), V266 (≠ T255), G308 (= G296), T309 (= T297)
5i5tA X-ray crystal structure at 2.31a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a tetrahydroquinoline compound and an internal aldimine linked plp cofactor. (see paper)
39% identity, 80% coverage: 23:307/355 of query aligns to 26:319/361 of 5i5tA
- active site: K198 (= K191)
- binding (3R)-3-methyl-1,2,3,4-tetrahydroquinoline-8-sulfonamide: F26 (= F23), F71 (= F68), Y169 (= Y166), K198 (= K191), T236 (= T230), A310 (= A298)
- binding pyridoxal-5'-phosphate: R95 (= R92), R188 (= R181), K198 (= K191), Y203 (= Y196), E233 (= E227), T236 (= T230), M237 (= M231), N238 (= N232), L262 (= L251), G264 (= G253), V265 (= V254), V266 (≠ T255), G308 (= G296), T309 (= T297)
5cr5A X-ray crystal structure at 1.61a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a biphenyl pyrrolidine ether compound and an internal aldimine linked plp cofactor. (see paper)
39% identity, 80% coverage: 23:307/355 of query aligns to 23:316/358 of 5cr5A
- active site: K195 (= K191)
- binding 3-({(3R)-1-[(5-bromothiophen-2-yl)carbonyl]pyrrolidin-3-yl}oxy)-N-methyl-2'-[(methylsulfonyl)amino]biphenyl-4-carboxamide: F23 (= F23), F68 (= F68), Y166 (= Y166), V175 (≠ L171), Q217 (= Q213), V231 (≠ S228), G232 (= G229), T233 (= T230), M234 (= M231), G305 (= G296), A307 (= A298), C311 (≠ A302)
- binding pyridoxal-5'-phosphate: R92 (= R92), K195 (= K191), Y200 (= Y196), E230 (= E227), T233 (= T230), N235 (= N232), L259 (= L251), G261 (= G253), V262 (= V254), V263 (≠ T255), T306 (= T297)
O15382 Branched-chain-amino-acid aminotransferase, mitochondrial; BCAT(m); Placental protein 18; PP18; EC 2.6.1.42 from Homo sapiens (Human) (see 6 papers)
39% identity, 80% coverage: 23:307/355 of query aligns to 57:350/392 of O15382
- Y168 (= Y134) binding
- R170 (= R136) to Q: in HVLI; reduced catalytic activity; dbSNP:rs749866079
- T186 (= T152) to R: in dbSNP:rs11548193
- K229 (= K191) modified: N6-(pyridoxal phosphate)lysine
- E264 (= E227) to K: in HVLI; reduced catalytic activity; dbSNP:rs767653663
- C342 (≠ A299) mutation to A: Reduces activity about 6-fold.
- C345 (≠ A302) mutation to A: Slight reduction of activity.
2hdkB Crystal structure of cys315ala-cys318ala mutant of human mitochondrial branched chain aminotransferase (see paper)
39% identity, 80% coverage: 23:307/355 of query aligns to 28:321/363 of 2hdkB
- active site: K200 (= K191)
- binding 2-oxo-4-methylpentanoic acid: G310 (= G296), T311 (= T297), A312 (= A298)
- binding pyridoxal-5'-phosphate: R97 (= R92), K200 (= K191), Y205 (= Y196), E235 (= E227), T238 (= T230), L264 (= L251), G266 (= G253), V267 (= V254), V268 (≠ T255), G310 (= G296), T311 (= T297)
5i5yA X-ray crystal structure at 1.81a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with an aryl acetate compound and an internal aldimine linked plp cofactor. (see paper)
39% identity, 80% coverage: 23:307/355 of query aligns to 28:321/363 of 5i5yA
- active site: K200 (= K191)
- binding {2-[(5-methyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-6-carbonyl)amino]phenyl}acetic acid: F28 (= F23), Y68 (= Y63), F73 (= F68), K77 (= K72), Y139 (= Y134), V153 (= V148), Y171 (= Y166), T238 (= T230), T311 (= T297), A312 (= A298)
- binding pyridoxal-5'-phosphate: R97 (= R92), R190 (= R181), K200 (= K191), Y205 (= Y196), E235 (= E227), T238 (= T230), L264 (= L251), G266 (= G253), V267 (= V254), V268 (≠ T255), T311 (= T297)
5i5uA X-ray crystal structure at 2.40a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a tetrahydronaphthalenyl compound and an internal aldimine linked plp cofactor. (see paper)
39% identity, 80% coverage: 23:307/355 of query aligns to 28:321/363 of 5i5uA
- active site: K200 (= K191)
- binding 2-hydroxy-N-[(1R)-1,2,3,4-tetrahydronaphthalen-1-yl]acetamide: F28 (= F23), Y68 (= Y63), F73 (= F68), V153 (= V148), K200 (= K191), Y205 (= Y196), T238 (= T230), G310 (= G296), T311 (= T297), A312 (= A298)
- binding pyridoxal-5'-phosphate: R97 (= R92), R190 (= R181), K200 (= K191), Y205 (= Y196), E235 (= E227), T238 (= T230), M239 (= M231), L264 (= L251), G266 (= G253), V267 (= V254), V268 (≠ T255), G310 (= G296), T311 (= T297)
2a1hA X-ray crystal structure of human mitochondrial branched chain aminotransferase (bcatm) complexed with gabapentin (see paper)
38% identity, 80% coverage: 23:307/355 of query aligns to 28:321/363 of 2a1hA
- active site: K200 (= K191)
- binding [1-(aminomethyl)cyclohexyl]acetic acid: Y68 (= Y63), R141 (= R136), V153 (= V148), T238 (= T230), G310 (= G296), T311 (= T297), A312 (= A298)
- binding pyridoxal-5'-phosphate: R97 (= R92), R190 (= R181), K200 (= K191), Y205 (= Y196), E235 (= E227), T238 (= T230), M239 (= M231), N240 (= N232), L264 (= L251), G266 (= G253), V267 (= V254), V268 (≠ T255), T311 (= T297)
1kt8A Human branched chain amino acid aminotransferase (mitochondrial): three dimensional structure of enzyme in its ketimine form with the substrate l-isoleucine (see paper)
39% identity, 80% coverage: 23:307/355 of query aligns to 30:318/360 of 1kt8A
- binding n-[o-phosphono-pyridoxyl]-isoleucine: G77 (= G70), R99 (= R92), Y141 (= Y134), R187 (= R181), K197 (= K191), Y202 (= Y196), E232 (= E227), T235 (= T230), N237 (= N232), G263 (= G253), V264 (= V254), V265 (≠ T255), G307 (= G296), T308 (= T297)
Query Sequence
>Echvi_1626 FitnessBrowser__Cola:Echvi_1626
MEIKRQLTSQCAIKQFNFNQFQFGVEATDHMLVARYSDGHWHSATIQPYEQLVLSPLAMC
LHYGQTVFEGLKAYRQEDDTISIFRLARHHERINQSLRRMAMPEIPEELFETGIRELVDL
EQEWIRGGEGNSLYIRPFVIATEARLGVSISTDYLFMVVCTPMAAYYAKPLKVKVERHYT
RAVPGGVGAAKNGGNYGAAYYPAHLAQQAGFDQVIWTDARDHQFVEESGTMNLMFIIDGV
LLTPPAGETVLAGVTRDSLLSIARDMGWPVEERPISLKELEEAFSTGKKVEAFGAGTAAV
VAPLELIQIDGQDFRPYIAEDAQMYQLKARLSAIRAGKEKDPYAWNTVLEKALTK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory