SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_1734 FitnessBrowser__Cola:Echvi_1734 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4l0oH Structure determination of cystathionine gamma-synthase from helicobacter pylori
47% identity, 100% coverage: 1:368/369 of query aligns to 1:373/373 of 4l0oH

query
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active site: R40 (= R42), Y92 (= Y94), D164 (= D165), K189 (= K190)
binding pyridoxal-5'-phosphate: Y38 (= Y40), R40 (= R42), S67 (= S69), G68 (= G70), L69 (≠ N71), Y92 (= Y94), D164 (= D165), T166 (= T167), S186 (= S187), T188 (= T189), K189 (= K190)

6cjaA Crystal structure of cystathionine beta-lyase from legionella pneumophila philadelphia 1 in complex with alanyl-plp and serine
45% identity, 98% coverage: 3:365/369 of query aligns to 4:380/381 of 6cjaA

query
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binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-alanine: Y45 (= Y40), R47 (= R42), S74 (= S69), G75 (= G70), M76 (≠ N71), I79 (≠ G74), Y99 (= Y94), E143 (= E136), N147 (= N140), D172 (= D165), T174 (= T167), S194 (= S187), T196 (= T189), K197 (= K190), S326 (= S319), L327 (= L320), T341 (≠ E334), R361 (= R346)
binding serine: E44 (≠ I39), Y45 (= Y40), R47 (= R42), T48 (≠ S43), Y99 (= Y94), R104 (≠ K99), N227 (≠ Q220), E325 (≠ T318), M340 (≠ V333), T341 (≠ E334)

4iyoB Crystal structure of cystathionine gamma lyase from xanthomonas oryzae pv. Oryzae (xometc) in complex with e-site serine, a-site serine, a- site external aldimine structure with aminoacrylate and a-site iminopropionate intermediates (see paper)
43% identity, 99% coverage: 1:365/369 of query aligns to 2:380/381 of 4iyoB

query
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4iyoB

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active site: R47 (= R42), Y99 (= Y94), D172 (= D165), K197 (= K190)
binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: Y45 (= Y40), R47 (= R42)
binding amino-acrylate: Y99 (= Y94), N147 (= N140), K197 (= K190), E325 (≠ T318), S326 (= S319), L327 (= L320), T341 (≠ E334), R361 (= R346)
binding pyruvic acid: Q221 (= Q214), F224 (≠ N217)
binding serine: Y45 (= Y40), R47 (= R42), T48 (≠ S43), Y99 (= Y94), R104 (≠ K99), N227 (≠ Q220), E325 (≠ T318)

4iy7B Crystal structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae in complex with e-site serine, a-site external aldimine structure with serine and a-site external aldimine structure with aminoacrylate intermediates (see paper)
43% identity, 99% coverage: 1:365/369 of query aligns to 2:380/381 of 4iy7B

query
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4iy7B

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H

Q

-

S

-

S

-

P

-

G

H

E

E

H

E

Q

S

G

L

F

I

E

Y
|
Y

S

S

R
|
R

S
x
T

Q

H

N

N

P

P

N

T

R

R

M

F

A

A

L

Y

E

E

E

R

L

C

L

V

A

A

Q

A

L

L

E

E

K

G

G

G

S

T

A

R

A

A

A

F

A

A

F

F

S

A

S
|
S

G
|
G

N
x
M

A

A

A

A

G

T

M

S

A

T

V

V

F

M

Q

E

A

L

L

L

P

D

L

A

G

G

S

S

H

H

I

V

V

V

A

A

P

M

S

D

D

D

M

L

Y
|
Y

H

G

G

G

L

T

K

F

K
x
R

Q

-

L

L

V

F

E

E

L

R

F

V

K

R

D

R

K

R

-

T

-

A

-

G

L

L

E

D

V

F

T

S

F

F

T

V

D

D

L

L

S

T

D

D

P

P

E

A

N

A

L

F

E

K

K

A

A

A

I

I

Q

R

P

A

N

D

T

T

K

K

L

M

L

V

W

W

I

I

E
|
E

T

T

P

P

S

T

N
|
N

P

P

M

M

L

L

K

K

I

L

S

V

D

D

I

I

R

A

R

A

L

I

T

A

K

V

M

I

A

A

K

R

E

K

D

H

D

G

I

L

R

L

V

T

V

V

C

V

D
|
D

N

N

T
|
T

F
|
F

A

A

T

S

P

P

V

M

F

L

Q

Q

N

R

P

P

L

L

E

S

L

L

G

G

A

A

D

D

L

L

V

V

M

V

H

H

S
|
S

A

A

T
|
T

K
|
K

Y

Y

F

L

G

N

G

G

H

H

S

S

D

D

I

M

L

V

G

G

G

G

A

I

L

A

I

V

T

V

K

G

K

D

S

N

D

A

E

E

F

L

W

A

K

E

Q
|
Q

I

M

V

A

N
x
F

V

L

Q

Q

Q
x
N

T

S

G

I

G

G

A

G

V

V

L

Q

S

G

P

P

F

F

D

D

C

S

Y

F

L

L

L

A

V

L

R

R

S

G

I

L

K

K

T

T

L

L

A

P

Y

L

R

R

M

M

R

R

G

A

H

H

A

C

E

E

H

N

A

A

G

L

M

A

I

L

A

A

T

Q

F

W

L

L

D

E

Q

T

H

H

P

P

K

A

V

I

E

E

R

K

V

V

F

I

Y

Y

P

P

G

G

L

L

T

A

A

S

H

H

P

P

G

Q

H

H

D

V

V

L

A

A

K

K

S

R

Q

Q

M

M

T

S

G

G

F

F

G

G

G

G

I

I

L

V

S

S

F

I

L

V

V

L

K

K

G

G

K

G

P

F

E

D

D

A

A

A

D

K

K

R

L

F

I

C

S

E

S

K

L

T

K

E

Y

L

Y

F

T

T

N

L

A

A

T
x
E

S
|
S

L
|
L

G

G

G

G

V

V

E

E

S

S

L

L

I

V

E

N

R

H

R

P

A

A

A

V

V

M

E
x
T

G

H

-

A

-

S

-

I

P

P

D

V

T

A

K

R

T

R

P

E

Q

Q

-

L

-

G

-

I

-

S

-

D

N

A

L

L

I

V

R
|
R

L

L

S

S

V

V

G

G

L

I

E

E

H

D

L

L

D

G

D

D

L

L

L

R

E

G

D

D

M

L

E

E

K

R

A

A

F

L

active site: R47 (= R42), Y99 (= Y94), D172 (= D165), K197 (= K190)
binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: Y45 (= Y40), R47 (= R42)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: S74 (= S69), G75 (= G70), M76 (≠ N71), Y99 (= Y94), E143 (= E136), N147 (= N140), D172 (= D165), T174 (= T167), F175 (= F168), S194 (= S187), T196 (= T189), K197 (= K190), E325 (≠ T318), S326 (= S319), L327 (= L320), T341 (≠ E334), R361 (= R346)
binding pyruvic acid: Q221 (= Q214), F224 (≠ N217)
binding serine: Y45 (= Y40), R47 (= R42), T48 (≠ S43), Y99 (= Y94), R104 (≠ K99), N227 (≠ Q220), E325 (≠ T318)

4iy7A Crystal structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae in complex with e-site serine, a-site external aldimine structure with serine and a-site external aldimine structure with aminoacrylate intermediates (see paper)
43% identity, 99% coverage: 1:365/369 of query aligns to 2:380/381 of 4iy7A

query
sites
4iy7A

M

L

K

S

F

L

E

A

T

T

L

L

A

A

I

I

H

H

G

G

G

G

E

Q

K

S

K

P

S

D

A

P

P

S

H

T

R

G

A

A

V

V

V

M

Q

P

P

P

I

I

T

Y

L

A

S

T

T

S

T

T

F

Y

E

A

H

Q

-

S

-

S

-

P

-

G

H

E

E

H

E

Q

S

G

L

F

I
x
E

Y
|
Y

S

S

R
|
R

S
x
T

Q

H

N

N

P

P

N

T

R

R

M

F

A

A

L

Y

E

E

E

R

L

C

L

V

A

A

Q

A

L

L

E

E

K

G

G

G

S

T

A

R

A

A

A

F

A

A

F

F

S

A

S
|
S

G
|
G

N
x
M

A

A

A

A

G

T

M

S

A

T

V

V

F

M

Q

E

A

L

L

L

P

D

L

A

G

G

S

S

H

H

I

V

V

V

A

A

P

M

S

D

D

D

M

L

Y
|
Y

H

G

G

G

L

T

K

F

K
x
R

Q

-

L

L

V

F

E

E

L

R

F

V

K

R

D

R

K

R

-

T

-

A

-

G

L

L

E

D

V

F

T

S

F

F

T

V

D

D

L

L

S

T

D

D

P

P

E

A

N

A

L

F

E

K

K

A

A

A

I

I

Q

R

P

A

N

D

T

T

K

K

L

M

L

V

W

W

I

I

E
|
E

T

T

P

P

S

T

N
|
N

P

P

M

M

L

L

K

K

I

L

S

V

D

D

I

I

R

A

R

A

L

I

T

A

K

V

M

I

A

A

K

R

E

K

D

H

D

G

I

L

R

L

V

T

V

V

C

V

D
|
D

N

N

T
|
T

F
|
F

A

A

T

S

P

P

V

M

F

L

Q

Q

N

R

P

P

L

L

E

S

L

L

G

G

A

A

D

D

L

L

V

V

M

V

H

H

S
|
S

A

A

T
|
T

K
|
K

Y

Y

F

L

G

N

G

G

H

H

S

S

D

D

I

M

L

V

G

G

G

G

A

I

L

A

I

V

T

V

K

G

K

D

S

N

D

A

E

E

F

L

W

A

K

E

Q

Q

I

M

V

A

N

F

V

L

Q

Q

Q
x
N

T

S

G

I

G

G

A

G

V

V

L

Q

S

G

P

P

F

F

D

D

C

S

Y

F

L

L

L

A

V

L

R

R

S

G

I

L

K

K

T

T

L

L

A

P

Y

L

R

R

M

M

R

R

G

A

H

H

A

C

E

E

H

N

A

A

G

L

M

A

I

L

A

A

T

Q

F

W

L

L

D

E

Q

T

H

H

P

P

K

A

V

I

E

E

R

K

V

V

F

I

Y

Y

P

P

G

G

L

L

T

A

A

S

H

H

P

P

G

Q

H

H

D

V

V

L

A

A

K

K

S

R

Q

Q

M

M

T

S

G

G

F

F

G

G

G

G

I

I

L

V

S

S

F

I

L

V

V

L

K

K

G

G

K

G

P

F

E

D

D

A

A

A

D

K

K

R

L

F

I

C

S

E

S

K

L

T

K

E

Y

L

Y

F

T

T

N

L

A

A

T
x
E

S
|
S

L
|
L

G

G

G

G

V

V

E

E

S

S

L

L

I

V

E

N

R

H

R

P

A

A

A

V

V

M

E
x
T

G

H

-

A

-

S

-

I

P

P

D

V

T

A

K

R

T

R

P

E

Q

Q

-

L

-

G

-

I

-

S

-

D

N

A

L

L

I

V

R
|
R

L

L

S

S

V

V

G

G

L

I

E

E

H

D

L

L

D

G

D

D

L

L

L

R

E

G

D

D

M

L

E

E

K

R

A

A

F

L

active site: R47 (= R42), Y99 (= Y94), D172 (= D165), K197 (= K190)
binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: S74 (= S69), G75 (= G70), M76 (≠ N71), Y99 (= Y94), E143 (= E136), N147 (= N140), D172 (= D165), T174 (= T167), F175 (= F168), S194 (= S187), T196 (= T189), K197 (= K190), S326 (= S319), L327 (= L320), T341 (≠ E334), R361 (= R346)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: Y45 (= Y40), R47 (= R42)
binding serine: E44 (≠ I39), Y45 (= Y40), R47 (= R42), T48 (≠ S43), Y99 (= Y94), R104 (≠ K99), N227 (≠ Q220), E325 (≠ T318)

4ixzA Native structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae at ph 9.0 (see paper)
43% identity, 99% coverage: 1:365/369 of query aligns to 2:380/381 of 4ixzA

query
sites
4ixzA

M

L

K

S

F

L

E

A

T

T

L

L

A

A

I

I

H

H

G

G

G

G

E

Q

K

S

K

P

S

D

A

P

P

S

H

T

R

G

A

A

V

V

V

M

Q

P

P

P

I

I

T

Y

L

A

S

T

T

S

T

T

F

Y

E

A

H

Q

-

S

-

S

-

P

-

G

H

E

E

H

E

Q

S

G

L

F

I

E

Y

Y

S

S

R
|
R

S

T

Q

H

N

N

P

P

N

T

R

R

M

F

A

A

L

Y

E

E

E

R

L

C

L

V

A

A

Q

A

L

L

E

E

K

G

G

G

S

T

A

R

A

A

A

F

A

A

F

F

S

A

S

S

G

G

N

M

A

A

A

A

G

T

M

S

A

T

V

V

F

M

Q

E

A

L

L

L

P

D

L

A

G

G

S

S

H

H

I

V

V

V

A

A

P

M

S

D

D

D

M

L

Y
|
Y

H

G

G

G

L

T

K

F

K

R

Q

-

L

L

V

F

E

E

L

R

F

V

K

R

D

R

K

R

-

T

-

A

-

G

L

L

E

D

V

F

T

S

F

F

T

V

D

D

L

L

S

T

D

D

P

P

E

A

N

A

L

F

E

K

K

A

A

A

I

I

Q

R

P

A

N

D

T

T

K

K

L

M

L

V

W

W

I

I

E

E

T

T

P

P

S

T

N
|
N

P

P

M

M

L

L

K

K

I

L

S

V

D

D

I

I

R

A

R

A

L

I

T

A

K

V

M

I

A

A

K

R

E

K

D

H

D

G

I

L

R

L

V

T

V

V

C

V

D
|
D

N

N

T

T

F

F

A

A

T

S

P

P

V

M

F

L

Q

Q

N

R

P

P

L

L

E

S

L

L

G

G

A

A

D

D

L

L

V

V

M

V

H

H

S

S

A

A

T

T

K
|
K

Y

Y

F

L

G

N

G

G

H

H

S

S

D

D

I

M

L

V

G

G

G

G

A

I

L

A

I

V

T

V

K

G

K

D

S

N

D

A

E

E

F

L

W

A

K

E

Q

Q

I

M

V

A

N

F

V

L

Q

Q

Q

N

T

S

G

I

G

G

A

G

V

V

L

Q

S

G

P

P

F

F

D

D

C

S

Y

F

L

L

L

A

V

L

R

R

S

G

I

L

K

K

T

T

L

L

A

P

Y

L

R

R

M

M

R

R

G

A

H

H

A

C

E

E

H

N

A

A

G

L

M

A

I

L

A

A

T

Q

F

W

L

L

D

E

Q

T

H

H

P

P

K

A

V

I

E

E

R

K

V

V

F

I

Y

Y

P

P

G

G

L

L

T

A

A

S

H

H

P

P

G

Q

H

H

D

V

V

L

A

A

K

K

S

R

Q

Q

M

M

T

S

G

G

F

F

G

G

G

G

I

I

L

V

S

S

F

I

L

V

V

L

K

K

G

G

K

G

P

F

E

D

D

A

A

A

D

K

K

R

L

F

I

C

S

E

S

K

L

T

K

E

Y

L

Y

F

T

T

N

L

A

A

T

E

S
|
S

L
|
L

G

G

G

G

V

V

E

E

S

S

L

L

I

V

E

N

R

H

R

P

A

A

A

V

V

M

E
x
T

G

H

-

A

-

S

-

I

P

P

D

V

T

A

K

R

T

R

P

E

Q

Q

-

L

-

G

-

I

-

S

-

D

N

A

L

L

I

V

R
|
R

L

L

S

S

V

V

G

G

L

I

E

E

H

D

L

L

D

G

D

D

L

L

L

R

E

G

D

D

M

L

E

E

K

R

A

A

F

L

active site: R47 (= R42), Y99 (= Y94), D172 (= D165), K197 (= K190)
binding bicarbonate ion: Y99 (= Y94), N147 (= N140), K197 (= K190), S326 (= S319), L327 (= L320), T341 (≠ E334), R361 (= R346)

4iyoD Crystal structure of cystathionine gamma lyase from xanthomonas oryzae pv. Oryzae (xometc) in complex with e-site serine, a-site serine, a- site external aldimine structure with aminoacrylate and a-site iminopropionate intermediates (see paper)
43% identity, 99% coverage: 1:365/369 of query aligns to 2:380/384 of 4iyoD

query
sites
4iyoD

M

L

K

S

F

L

E

A

T

T

L

L

A

A

I

I

H

H

G

G

G

G

E

Q

K

S

K

P

S

D

A

P

P

S

H

T

R

G

A

A

V

V

V

M

Q

P

P

P

I

I

T

Y

L

A

S

T

T

S

T

T

F

Y

E

A

H

Q

-

S

-

S

-

P

-

G

H

E

E

H

E

Q

S

G

L

F

I

E

Y
|
Y

S

S

R
|
R

S
x
T

Q

H

N

N

P

P

N

T

R

R

M

F

A

A

L

Y

E

E

E

R

L

C

L

V

A

A

Q

A

L

L

E

E

K

G

G

G

S

T

A

R

A

A

A

F

A

A

F

F

S

A

S

S

G

G

N

M

A

A

A

A

G

T

M

S

A

T

V

V

F

M

Q

E

A

L

L

L

P

D

L

A

G

G

S

S

H

H

I

V

V

V

A

A

P

M

S

D

D

D

M

L

Y
|
Y

H

G

G

G

L

T

K

F

K
x
R

Q

-

L

L

V

F

E

E

L

R

F

V

K

R

D

R

K

R

-

T

-

A

-

G

L

L

E

D

V

F

T

S

F

F

T

V

D

D

L

L

S

T

D

D

P

P

E

A

N

A

L

F

E

K

K

A

A

A

I

I

Q

R

P

A

N

D

T

T

K

K

L

M

L

V

W

W

I

I

E

E

T

T

P

P

S

T

N
|
N

P

P

M

M

L

L

K

K

I

L

S

V

D

D

I

I

R

A

R

A

L

I

T

A

K

V

M

I

A

A

K

R

E

K

D

H

D

G

I

L

R

L

V

T

V

V

C

V

D
|
D

N

N

T

T

F

F

A

A

T

S

P

P

V

M

F

L

Q

Q

N

R

P

P

L

L

E

S

L

L

G

G

A

A

D

D

L

L

V

V

M

V

H

H

S

S

A

A

T

T

K
|
K

Y

Y

F

L

G

N

G

G

H

H

S

S

D

D

I

M

L

V

G

G

G

G

A

I

L

A

I

V

T

V

K

G

K

D

S

N

D

A

E

E

F

L

W

A

K

E

Q

Q

I

M

V

A

N

F

V

L

Q

Q

Q
x
N

T

S

G

I

G

G

A

G

V

V

L

Q

S

G

P

P

F

F

D

D

C

S

Y

F

L

L

L

A

V

L

R

R

S

G

I

L

K

K

T

T

L

L

A

P

Y

L

R

R

M

M

R

R

G

A

H

H

A

C

E

E

H

N

A

A

G

L

M

A

I

L

A

A

T

Q

F

W

L

L

D

E

Q

T

H

H

P

P

K

A

V

I

E

E

R

K

V

V

F

I

Y

Y

P

P

G

G

L

L

T

A

A

S

H

H

P

P

G

Q

H

H

D

V

V

L

A

A

K

K

S

R

Q

Q

M

M

T

S

G

G

F

F

G

G

G

G

I

I

L

V

S

S

F

I

L

V

V

L

K

K

G

G

K

G

P

F

E

D

D

A

A

A

D

K

K

R

L

F

I

C

S

E

S

K

L

T

K

E

Y

L

Y

F

T

T

N

L

A

A

T
x
E

S
|
S

L
|
L

G

G

G

G

V

V

E

E

S

S

L

L

I

V

E

N

R

H

R

P

A

A

A

V

V

M

E
x
T

G

H

-

A

-

S

-

I

P

P

D

V

T

A

K

R

T

R

P

E

Q

Q

-

L

-

G

-

I

-

S

-

D

N

A

L

L

I

V

R
|
R

L

L

S

S

V

V

G

G

L

I

E

E

H

D

L

L

D

G

D

D

L

L

L

R

E

G

D

D

M

L

E

E

K

R

A

A

F

L

active site: R47 (= R42), Y99 (= Y94), D172 (= D165), K197 (= K190)
binding serine: Y45 (= Y40), R47 (= R42), T48 (≠ S43), Y99 (= Y94), Y99 (= Y94), R104 (≠ K99), N147 (= N140), K197 (= K190), N227 (≠ Q220), E325 (≠ T318), E325 (≠ T318), S326 (= S319), L327 (= L320), T341 (≠ E334), R361 (= R346)

4ixsB Native structure of xometc at ph 5.2 (see paper)
44% identity, 99% coverage: 1:365/369 of query aligns to 1:371/372 of 4ixsB

query
sites
4ixsB

M

L

K

S

F

L

E

A

T

T

L

L

A

A

I

I

H

H

G

G

G

G

E

Q

K

S

K

P

S

D

A

P

P

S

H

T

R

G

A

A

V

V

V

M

Q

P

P

P

I

I

T

Y

L

A

S

T

T

S

T

T

F

Y

E

A

H

Q

H

S

E

S

E

P

S

S

L

-

I

-

Y

-

S

-

R
|
R

S

T

Q

H

N

N

P

P

N

T

R

R

M

F

A

A

L

Y

E

E

E

R

L

C

L

V

A

A

Q

A

L

L

E

E

K

G

G

G

S

T

A

R

A

A

A

F

A

A

F

F

S

A

S

S

G

G

N

M

A

A

A

A

G

T

M

S

A

T

V

V

F

M

Q

E

A

L

L

L

P

D

L

A

G

G

S

S

H

H

I

V

V

V

A

A

P

M

S

D

D

D

M

L

Y
|
Y

H

G

G

G

L

T

K

F

K

R

Q

-

L

L

V

F

E

E

L

R

F

V

K

R

D

R

K

R

-

T

-

A

-

G

L

L

E

D

V

F

T

S

F

F

T

V

D

D

L

L

S

T

D

D

P

P

E

A

N

A

L

F

E

K

K

A

A

A

I

I

Q

R

P

A

N

D

T

T

K

K

L

M

L

V

W

W

I

I

E

E

T

T

P

P

S

T

N

N

P

P

M

M

L

L

K

K

I

L

S

V

D

D

I

I

R

A

R

A

L

I

T

A

K

V

M

I

A

A

K

R

E

K

D

H

D

G

I

L

R

L

V

T

V

V

C

V

D
|
D

N

N

T

T

F

F

A

A

T

S

P

P

V

M

F

L

Q

Q

N

R

P

P

L

L

E

S

L

L

G

G

A

A

D

D

L

L

V

V

M

V

H

H

S

S

A

A

T

T

K
|
K

Y

Y

F

L

G

N

G

G

H

H

S

S

D

D

I

M

L

V

G

G

G

G

A

I

L

A

I

V

T

V

K

G

K

D

S

N

D

A

E

E

F

L

W

A

K

E

Q

Q

I

M

V

A

N

F

V

L

Q

Q

Q

N

T

S

G

I

G

G

A

G

V

V

L

Q

S

G

P

P

F

F

D

D

C

S

Y

F

L

L

L

A

V

L

R

R

S

G

I

L

K

K

T

T

L

L

A

P

Y

L

R

R

M

M

R

R

G

A

H

H

A

C

E

E

H

N

A

A

G

L

M

A

I

L

A

A

T

Q

F

W

L

L

D

E

Q

T

H

H

P

P

K

A

V

I

E

E

R

K

V

V

F

I

Y

Y

P

P

G

G

L

L

T

A

A

S

H

H

P

P

G

Q

H

H

D

V

V

L

A

A

K

K

S

R

Q

Q

M

M

T

S

G

G

F

F

G

G

G

G

I

I

L

V

S

S

F

I

L

V

V

L

K

K

G

G

K

G

P

F

E

D

D

A

A

A

D

K

K

R

L

F

I

C

S

E

S

K

L

T

K

E

Y

L

Y

F

T

T

N

L

A

A

T
x
E

S
|
S

L
|
L

G

G

G

G

V

V

E

E

S

S

L

L

I

V

E

N

R

H

R

P

A

A

A

V

V

M

E
x
T

G

H

-

A

-

S

-

I

P

P

D

V

T

A

K

R

T

R

P

E

Q

Q

-

L

-

G

-

I

-

S

-

D

N

A

L

L

I

V

R
|
R

L

L

S

S

V

V

G

G

L

I

E

E

H

D

L

L

D

G

D

D

L

L

L

R

E

G

D

D

M

L

E

E

K

R

A

A

F

L

active site: R38 (= R42), Y90 (= Y94), D163 (= D165), K188 (= K190)
binding carbonate ion: Y90 (= Y94), K188 (= K190), E316 (≠ T318), S317 (= S319), L318 (= L320), T332 (≠ E334), R352 (= R346)

6k1lB E53a mutant of a putative cystathionine gamma-lyase (see paper)
43% identity, 99% coverage: 1:365/369 of query aligns to 3:381/382 of 6k1lB

query
sites
6k1lB

M

L

K

A

F

L

E

A

T

T

L

L

A

A

I

I

H

H

G

G

G

G

E

Q

K

S

K

P

S

D

A

P

P

S

H

T

R

G

A

A

V

V

V

M

Q

P

P

P

I

I

T

Y

L

A

S

T

T

S

T

T

F

Y

E

A

H

Q

-

S

-

S

-

P

-

G

H

E

E

H

E

Q

S

G

L

F

I

A

Y
|
Y

S

S

R
|
R

S

T

Q

H

N

N

P

P

N

T

R

R

M

F

A

A

L

Y

E

E

E

R

L

C

L

V

A

A

Q

S

L

L

E

E

K

G

G

G

S

T

A

R

A

G

A

F

A

A

F

F

S

A

S
|
S

G
|
G

N
x
M

A

A

A

A

G

S

M

S

A

T

V

V

F

I

Q

E

A

L

L

L

P

D

L

A

G

G

S

S

H

H

I

V

V

V

A

A

P

M

S

D

D

D

M

I

Y
|
Y

H

G

G

G

L

-

K

S

K

F

Q

R

L

L

V

F

E

E

L

R

F

V

K

R

D

R

K

R

-

T

-

A

-

G

L

L

E

D

V

F

T

S

F

F

T

V

D

D

L

L

S

T

D

D

P

L

E

A

N

A

L

F

E

E

K

A

A

S

I

I

Q

T

P

P

N

K

T

T

K

K

L

M

L

V

W

W

I

I

E
|
E

T

T

P

P

S

T

N
|
N

P

P

M

M

L

L

K

K

I

I

S

V

D

D

I

I

R

A

R

A

L

V

T

A

K

A

M

I

A

A

K

K

E

R

D

H

D

G

I

L

R

I

V

V

V

V

C

V

D
|
D

N

N

T
|
T

F

F

A

A

T

S

P

P

V

M

F

L

Q

Q

N

R

P

P

L

L

E

E

L

L

G

G

A

A

D

D

L

L

V

V

M

L

H

H

S
|
S

A

A

T
|
T

K
|
K

Y

Y

F

L

G

N

G

G

H

H

S

S

D

D

I

M

L

V

G
|
G

G

G

A

M

L

V

I

V

T

V

K

G

K

D

S

N

D

A

E

E

F

L

W

A

K

E

Q

Q

I

M

V

A

N

F

V

L

Q

Q

Q

N

T

S

G

V

G

G

A

G

V

V

L

Q

S

G

P

P

F

F

D

D

C

S

Y

F

L

L

L

A

V

L

R

R

S

G

I

L

K

K

T

T

L

L

A

P

Y

L

R

R

M

M

R

K

G

A

H

H

A

C

E

A

H

N

A

A

G

L

M

A

I

L

A

A

T

Q

F

W

L

L

D

E

Q

K

H

H

P

P

K

A

V

V

E

E

R

K

V

V

F

I

Y

Y

P

P

G

G

L

L

T

A

A

S

H

H

P

P

G

Q

H

H

D

E

V

L

A

A

K

G

S

K

Q

Q

M

M

T

A

G

G

F

Y

G

G

G

G

I

I

L

V

S

S

F

I

L

V

V

L

K

K

G

G

K

G

P

F

E

D

D

A

A

A

D

K

K

R

L

F

I

C

S

E

S

K

L

T

K

E

Y

L

Y

F

T

T

N

L

A

A

T
x
E

S
|
S

L
|
L

G

G

G

G

V

V

E

E

S

S

L

L

I

V

E

N

R

H

R

P

A

A

A

V

V

M

E
x
T

G

H

-

A

-

S

-

I

P

P

D

V

T

A

K

R

T

R

P

E

Q

Q

-

L

-

G

-

I

-

S

-

D

N

A

L

L

I

V

R
|
R

L

L

S

S

V

V

G

G

L

V

E

E

H

D

L

L

D

G

D

D

L

L

L

Q

E

V

D

D

M

L

E

G

K

E

A

A

F

L

binding pyridoxal-5'-phosphate: Y46 (= Y40), R48 (= R42), S75 (= S69), G76 (= G70), M77 (≠ N71), Y100 (= Y94), E144 (= E136), D173 (= D165), T175 (= T167), S195 (= S187), T197 (= T189), K198 (= K190), G208 (= G200)
binding pyruvic acid: Y100 (= Y94), N148 (= N140), K198 (= K190), E326 (≠ T318), S327 (= S319), L328 (= L320), T342 (≠ E334), R362 (= R346)

6k1lA E53a mutant of a putative cystathionine gamma-lyase (see paper)
43% identity, 99% coverage: 1:365/369 of query aligns to 3:381/382 of 6k1lA

query
sites
6k1lA

M

L

K

A

F

L

E

A

T

T

L

L

A

A

I

I

H

H

G

G

G

G

E

Q

K

S

K

P

S

D

A

P

P

S

H

T

R

G

A

A

V

V

V

M

Q

P

P

P

I

I

T

Y

L

A

S

T

T

S

T

T

F

Y

E

A

H

Q

-

S

-

S

-

P

-

G

H

E

E

H

E

Q

S

G

L

F

I

A

Y
|
Y

S

S

R
|
R

S

T

Q

H

N

N

P

P

N

T

R

R

M

F

A

A

L

Y

E

E

E

R

L

C

L

V

A

A

Q

S

L

L

E

E

K

G

G

G

S

T

A

R

A

G

A

F

A

A

F

F

S

A

S
|
S

G
|
G

N
x
M

A

A

A

A

G

S

M

S

A

T

V

V

F

I

Q

E

A

L

L

L

P

D

L

A

G

G

S

S

H

H

I

V

V

V

A

A

P

M

S

D

D

D

M

I

Y
|
Y

H

G

G

G

L

-

K

S

K

F

Q

R

L

L

V

F

E

E

L

R

F

V

K

R

D

R

K

R

-

T

-

A

-

G

L

L

E

D

V

F

T

S

F

F

T

V

D

D

L

L

S

T

D

D

P

L

E

A

N

A

L

F

E

E

K

A

A

S

I

I

Q

T

P

P

N

K

T

T

K

K

L

M

L

V

W

W

I

I

E
|
E

T

T

P

P

S

T

N

N

P

P

M

M

L

L

K

K

I

I

S

V

D

D

I

I

R

A

R

A

L

V

T

A

K

A

M

I

A

A

K

K

E

R

D

H

D

G

I

L

R

I

V

V

V

V

C

V

D
|
D

N

N

T
|
T

F

F

A

A

T

S

P

P

V

M

F

L

Q

Q

N

R

P

P

L

L

E

E

L

L

G

G

A

A

D

D

L

L

V

V

M

L

H

H

S
|
S

A

A

T
|
T

K
|
K

Y

Y

F

L

G

N

G

G

H

H

S

S

D

D

I

M

L

V

G
|
G

G

G

A

M

L

V

I

V

T

V

K

G

K

D

S

N

D

A

E

E

F

L

W

A

K

E

Q

Q

I

M

V

A

N

F

V

L

Q

Q

Q

N

T

S

G

V

G

G

A

G

V

V

L

Q

S

G

P

P

F

F

D

D

C

S

Y

F

L

L

L

A

V

L

R

R

S

G

I

L

K

K

T

T

L

L

A

P

Y

L

R

R

M

M

R

K

G

A

H

H

A

C

E

A

H

N

A

A

G

L

M

A

I

L

A

A

T

Q

F

W

L

L

D

E

Q

K

H

H

P

P

K

A

V

V

E

E

R

K

V

V

F

I

Y

Y

P

P

G

G

L

L

T

A

A

S

H

H

P

P

G

Q

H

H

D

E

V

L

A

A

K

G

S

K

Q

Q

M

M

T

A

G

G

F

Y

G

G

G

G

I

I

L

V

S

S

F

I

L

V

V

L

K

K

G

G

K

G

P

F

E

D

D

A

A

A

D

K

K

R

L

F

I

C

S

E

S

K

L

T

K

E

Y

L

Y

F

T

T

N

L

A

A

T

E

S

S

L

L

G

G

G

G

V

V

E

E

S

S

L

L

I

V

E

N

R

H

R

P

A

A

A

V

V

M

E

T

G

H

-

A

-

S

-

I

P

P

D

V

T

A

K

R

T

R

P

E

Q

Q

-

L

-

G

-

I

-

S

-

D

N

A

L

L

I

V

R

R

L

L

S

S

V

V

G

G

L

V

E

E

H

D

L

L

D

G

D

D

L

L

L

Q

E

V

D

D

M

L

E

G

K

E

A

A

F

L

binding pyridoxal-5'-phosphate: Y46 (= Y40), R48 (= R42), S75 (= S69), G76 (= G70), M77 (≠ N71), Y100 (= Y94), E144 (= E136), D173 (= D165), T175 (= T167), S195 (= S187), T197 (= T189), K198 (= K190), G208 (= G200)

7d7oB Crystal structure of cystathionine gamma-lyase from bacillus cereus atcc 14579 (see paper)
41% identity, 99% coverage: 1:365/369 of query aligns to 1:376/377 of 7d7oB

query
sites
7d7oB

M

M

K

R

F

A

E

K

T

T

L

K

A

L

I

I

H

H

G

G

G

I

E

R

K

I

K

G

S

E

A

P

P

S

H

T

R

G

A

S

V

V

V

N

Q

V

P

P

I

I

T

Y

L

Q

S

T

T

S

T

T

F

Y

E

K

H

Q

H

E

-

A

-

V

-

G

-

K

E

H

E

Q

S

G

L

Y

I

E

Y
|
Y

S

S

R
|
R

S

T

Q

G

N

N

P

P

N

T

R

R

M

A

A

A

L

L

E

E

E

E

L

M

L

I

A

A

Q

V

L

L

E

E

K

N

G

G

S

H

A

A

A

G

A

F

A

A

F

F

S

G

S
|
S

G
|
G

N
x
M

A

A

A

A

G

I

M

T

A

A

V

T

F

I

Q

M

A

L

L

F

P

S

L

K

G

G

S

D

H

H

I

V

V

I

A

L

P

T

S

D

D

D

M

V

Y
|
Y

H

G

G

G

L

T

K

Y

K

R

Q

V

L

I

V

T

E

K

L

V

F

L

-

N

K

R

D

F

K

G

L

I

E

E

V

H

T

T

F

F

T

V

D

D

L

T

S

T

D

N

P

L

E

E

N

E

L

V

E

E

K

E

A

A

I

I

Q

R

P

P

N

N

T

T

K

K

L

A

L

I

W

Y

I

V

E
|
E

T

T

P

P

S

T

N

N

P

P

M

L

L

L

K

K

I

I

S

T

D

D

I

I

R

K

R

K

L

I

T

S

K

T

M

L

A

A

K

K

E

E

D

K

D

D

I

L

R

L

V

T

V

I

C

I

D
|
D

N

N

T
|
T

F

F

A

M

T

T

P

P

V

Y

F

W

Q

Q

N

S

P

P

L

I

E

S

L

L

G

G

A

A

D

D

L

I

V

V

M

L

H

H

S
|
S

A

A

T
|
T

K
|
K

Y

Y

F

L

G

G

G

G

H

H

S

S

D

D

I

V

L

V

G

A

G

G

A

-

L

L

I

V

T

V

K

V

K

N

S

S

D

P

E

Q

F

L

W

A

K

E

Q

D

I

L

V

H

N

F

V

V

Q

Q

Q

N

T

S

G

T

G

G

A

G

V

I

L

L

S

G

P

P

F

Q

D

D

C

S

Y

F

L

L

L

L

V

L

R

R

S

G

I

L

K

K

T

T

L

L

A

G

Y

I

R

R

M

M

R

E

G

E

H

H

A

E

E

T

H

N

A

S

G

R

M

A

I

I

A

A

T

E

F

F

L

L

D

N

Q

N

H

H

P

P

K

K

V

V

E

N

R

K

V

V

F

Y

Y

Y

P

P

G

G

L

L

T

E

A

S

H

H

P

Q

G

N

H

H

D

E

V

L

A

A

K

T

S

E

Q

Q

M

A

T

N

G

G

F

F

G

G

G

A

I

I

L

I

S

S

F

F

L

D

V

V

K

D

G

S

K

E

P

-

E

E

D

T

A

L

D

N

K

K

L

V

I

L

S

E

S

K

L

L

K

Q

Y

Y

Y

F

T

T

N

L

A

A

T

E

S

S

L

L

G

G

G

A

V

V

E

E

S

S

L

L

I

I

-

S

-

I

-

P

-

S

-

Q

-

M

-

T

-

H

-

A

-

S

-

I

-

P

-

A

E

D

R

R

R

R

A

K

A

E

V

L

E

G

G

I

P

T

D

D

T

T

K

-

T

-

P

-

Q

-

N

-

L

L

I

I

R

R

L

I

S

S

V

V

G

G

L

I

E

E

H

D

L

G

D

E

D

D

L

L

L

I

E

E

D

D

M

L

E

A

K

Q

A

A

F

L

binding pyridoxal-5'-phosphate: Y44 (= Y40), R46 (= R42), S73 (= S69), G74 (= G70), M75 (≠ N71), Y98 (= Y94), E141 (= E136), D170 (= D165), T172 (= T167), S192 (= S187), T194 (= T189), K195 (= K190)

7ba4A Structure of cystathionine gamma-lyase from pseudomonas aeruginosa
40% identity, 99% coverage: 3:368/369 of query aligns to 7:374/377 of 7ba4A

query
sites
7ba4A

F

F

E

A

T

T

L

R

A

V

I

I

H

H

G

A

G

G

E

Q

K

A

K

P

S

D

A

P

P

S

H

T

R

G

A

A

V

I

V

M

Q

P

P

P

I

I

T

Y

L

A

S

N

T

S

T

T

F

Y

E

-

H

-

H

-

E

-

E

-

S

-

L

-

I

-

Y

I

S

Q

R

R

S

S

Q

H

N

N

P

P

N

T

R

R

M

W

A

A

L

L

E

E

E

R

L

C

L

V

A

A

Q

D

L

L

E

E

K

G

G

G

S

T

A

Q

A

A

A

F

A

A

F

F

S

A

S
|
S

G
|
G

N
x
L

A

A

A

A

G

I

M

S

A

S

V

V

F

L

Q

E

A

L

L

L

P

D

L

A

G

G

S

S

H

H

I

I

V

V

A

S

P

G

S

N

D

D

M

L

Y
|
Y

H

G

G

G

L

T

K

F

K

R

Q

-

L

L

V

F

E

E

L

R

F

V

K

R

D

R

K

R

L

S

-

A

-

G

-

H

E

R

V

F

T

S

F

F

T

V

D

D

L

P

S

T

D

D

P

L

E

Q

N

A

L

F

E

E

K

A

A

A

I

L

Q

T

P

P

N

E

T

T

K

R

L

M

L

V

W

W

I

V

E
|
E

T

T

P

P

S

S

N

N

P

P

M

L

L

L

K

R

I

L

S

T

D

D

I

L

R

R

R

A

L

I

T

A

K

Q

M

L

A

C

K

R

E

A

D

R

D

G

I

I

R

I

V

S

V

V

C

A

D
|
D

N

N

T
|
T

F
|
F

A

A

T

S

P

P

V

Y

F

I

Q

Q

N

R

P

P

L

L

E

E

L

L

G

G

A

F

D

D

L

V

V

V

M

V

H

H

S
|
S

A

T

T