SitesBLAST

1a56A Primary sequence and solution conformation of ferricytochromE C-552 from nitrosomonas europaea, nmr, mean structure refined with explicit hydrogen bond constraints (see paper)
47% identity, 55% coverage: 63:140/141 of query aligns to 4:81/81 of 1a56A

query
sites
1a56A

L

L

V

A

K

K

E

K

S

N

D
x
N

C
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C

P

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C

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H

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V

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E

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K

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A

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Y

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A

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A

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K

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binding heme c: N9 (≠ D68), C10 (= C69), C13 (= C72), H14 (= H73), V21 (= V80), G22 (= G81), P23 (= P82), K45 (≠ R104), I46 (≠ V105), S50 (≠ N109), W54 (= W113), Q56 (= Q115), I57 (≠ V116), M59 (= M118), N62 (≠ H121), V63 (≠ P122), N64 (≠ G123)

1corA Investigation of the solution conformation of cytochromE C-551 from pseudomonas stutzeri (see paper)
43% identity, 58% coverage: 59:140/141 of query aligns to 2:82/82 of 1corA

query
sites
1corA

E

D

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A

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N

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binding heme c: K10 (≠ S67), C12 (= C69), C15 (= C72), H16 (= H73), V23 (= V80), G24 (= G81), P25 (= P82), L44 (= L101), H47 (≠ R104), I48 (≠ V105), S52 (≠ N109), Q53 (≠ N110), G54 (= G111), V55 (= V112), W56 (= W113), G57 (= G114), I59 (≠ V116), M61 (= M118), N64 (≠ H121)

1cchA The solution conformation of cytochromE C-551 from p.Stutzeri zobell determined by nmr+ (see paper)
43% identity, 58% coverage: 59:140/141 of query aligns to 2:82/82 of 1cchA

query
sites
1cchA

E

D

G

G

L

E

A

A

L

L

V

F

K

K

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K

D

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C
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C

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A

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C

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H

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V

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D

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K

K

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V

G

G

P

P

A

A

Y

L

K

K

D

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V

A

A

E

A

K

K

Y

N

E

A

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T

V

D

E

E

G

N

A

I

A

E

D

T

T

L
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L

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A

K

L

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x
H

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K

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N

G

G

N
x
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x
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W

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G

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M

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x
N

P

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G

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L

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E

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M

L

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binding heme c: C12 (= C69), C15 (= C72), H16 (= H73), L44 (= L101), H47 (≠ R104), I48 (≠ V105), S52 (≠ N109), Q53 (≠ N110), V55 (= V112), W56 (= W113), G57 (= G114), I59 (≠ V116), M61 (= M118), P62 (= P119), N64 (≠ H121)

P00099 Cytochrome c-551; Cytochrome C8; Cytochrome c551 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
42% identity, 55% coverage: 63:140/141 of query aligns to 28:104/104 of P00099

query
sites
P00099

L
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L

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x
F

K
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K

E
x
N

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x
K

D
x
G

C
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C

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C

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H

M
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A

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I

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x
D

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x
T

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K

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M

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V

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G

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P

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A

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Y

K
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K

D
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D

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V

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A

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x
A

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K

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F

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G

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R

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G

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K

H38 (= H73) binding axial binding residue
M83 (= M118) binding axial binding residue

Sites not aligning to the query:

1:22 signal peptide
23:104 modified: mature protein, Cytochrome c-551

351cA Structure of cytochrome c551 from p. Aeruginosa refined at 1.6 angstroms resolution and comparison of the two redox forms (see paper)
42% identity, 55% coverage: 63:140/141 of query aligns to 6:82/82 of 351cA

query
sites
351cA

L

L

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K

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binding protoporphyrin ix containing fe: C12 (= C69), C15 (= C72), H16 (= H73), G24 (= G81), P25 (= P82), Y27 (= Y84), L44 (= L101), R47 (= R104), I48 (≠ V105), Q53 (≠ N110), G54 (= G111), V55 (= V112), W56 (= W113), G57 (= G114), I59 (≠ V116), P60 (= P117), M61 (= M118)

2exvA Crystal structure of the f7a mutant of the cytochrome c551 from pseudomonas aeruginosa (see paper)
42% identity, 55% coverage: 63:140/141 of query aligns to 6:82/82 of 2exvA

query
sites
2exvA

L

L

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K

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binding heme c: C12 (= C69), C15 (= C72), H16 (= H73), G24 (= G81), P25 (= P82), R47 (= R104), I48 (≠ V105), S52 (≠ N109), Q53 (≠ N110), G54 (= G111), V55 (= V112), W56 (= W113), G57 (= G114), I59 (≠ V116), P60 (= P117), M61 (= M118), N64 (≠ H121)

6kq1A Crystal structure of cytochrome c551 from pseudomonas sp. Strain mt-1.
40% identity, 58% coverage: 59:140/141 of query aligns to 2:82/82 of 6kq1A

query
sites
6kq1A

E
x
D

G

G

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x
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A

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binding heme c: C12 (= C69), C15 (= C72), H16 (= H73), G24 (= G81), P25 (= P82), V30 (= V87), Y34 (= Y91), L44 (= L101), H47 (≠ R104), I48 (≠ V105), Q53 (≠ N110), G54 (= G111), N55 (≠ V112), W56 (= W113), G57 (= G114), I59 (≠ V116), M61 (= M118), N64 (≠ H121)
binding zinc ion: D2 (≠ E59), E4 (≠ L61), K10 (≠ S67), E70 (≠ D128)

5xecC Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
42% identity, 55% coverage: 63:140/141 of query aligns to 4:80/80 of 5xecC

query
sites
5xecC

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binding heme c: C10 (= C69), C13 (= C72), H14 (= H73), P23 (= P82), Y25 (= Y84), L42 (= L101), R45 (= R104), Q51 (≠ N110), V53 (= V112), W54 (= W113), G55 (= G114), I57 (≠ V116), M59 (= M118), N62 (≠ H121)

5xecA Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
41% identity, 55% coverage: 63:140/141 of query aligns to 6:82/82 of 5xecA

query
sites
5xecA

L

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binding heme c: C12 (= C69), C15 (= C72), H16 (= H73), G24 (= G81), P25 (= P82), Y27 (= Y84), Y34 (= Y91), Y43 (≠ T100), I48 (≠ V105), S53 (≠ N110), G54 (= G111), V55 (= V112), W56 (= W113), G57 (= G114), V59 (= V116), M61 (= M118), Q64 (≠ H121), I78 (= I136)

5aurA Hydrogenobacter thermophilus cytochrome c552 dimer formed by domain swapping at n-terminal region (see paper)
40% identity, 55% coverage: 63:140/141 of query aligns to 4:83/83 of 5aurA

query
sites
5aurA

L

L

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binding heme c: C10 (= C69), C13 (= C72), H14 (= H73), P26 (= P82), Y28 (= Y84), Y35 (= Y91), Y44 (≠ T100), I49 (≠ V105), S54 (≠ N110), G55 (= G111), V56 (= V112), W57 (= W113), G58 (= G114), V60 (= V116), P61 (= P117), M62 (= M118), Q65 (≠ H121)

Query Sequence

>Echvi_1841 FitnessBrowser__Cola:Echvi_1841
MNLSKLASAGAIALAGMAYACGGGSDTKSEETTSAAESAAPKKEMSFDEMYKDNPDYVEG
LALVKESDCPSCHMVERKIVGPAYKDVAEKYESTDENIETLAKRVVDGNNGVWGQVPMPA
HPGLSEDDAKKMVKYILMLKK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory