SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_2080 FitnessBrowser__Cola:Echvi_2080 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7d7oB Crystal structure of cystathionine gamma-lyase from bacillus cereus atcc 14579 (see paper)
56% identity, 99% coverage: 1:378/381 of query aligns to 1:376/377 of 7d7oB

query
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7d7oB

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binding pyridoxal-5'-phosphate: Y44 (= Y44), R46 (= R46), G74 (= G74), M75 (= M75), Y98 (= Y98), D170 (= D170), T172 (= T172), S192 (= S192), T194 (= T194), K195 (= K195)

4iyoB Crystal structure of cystathionine gamma lyase from xanthomonas oryzae pv. Oryzae (xometc) in complex with e-site serine, a-site serine, a- site external aldimine structure with aminoacrylate and a-site iminopropionate intermediates (see paper)
54% identity, 99% coverage: 1:378/381 of query aligns to 2:380/381 of 4iyoB

query
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4iyoB

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active site: R47 (= R46), Y99 (= Y98), D172 (= D170), K197 (= K195)
binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: Y45 (= Y44), R47 (= R46)
binding amino-acrylate: Y99 (= Y98), K197 (= K195), S326 (= S324), T341 (= T339), R361 (= R359)
binding pyruvic acid: Q221 (≠ D218), F224 (= F221)
binding serine: Y45 (= Y44), T48 (= T47), Y99 (= Y98), R104 (= R103), N227 (= N224), E325 (= E323)

4iy7B Crystal structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae in complex with e-site serine, a-site external aldimine structure with serine and a-site external aldimine structure with aminoacrylate intermediates (see paper)
54% identity, 99% coverage: 1:378/381 of query aligns to 2:380/381 of 4iy7B

query
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4iy7B

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active site: R47 (= R46), Y99 (= Y98), D172 (= D170), K197 (= K195)
binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: Y45 (= Y44), R47 (= R46)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: G75 (= G74), M76 (= M75), Y99 (= Y98), E143 (= E141), N147 (= N145), D172 (= D170), S194 (= S192), K197 (= K195), S326 (= S324), L327 (= L325), T341 (= T339), R361 (= R359)
binding pyruvic acid: Q221 (≠ D218), F224 (= F221)
binding serine: Y45 (= Y44), T48 (= T47), Y99 (= Y98), R104 (= R103), N227 (= N224), E325 (= E323)

4iy7A Crystal structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae in complex with e-site serine, a-site external aldimine structure with serine and a-site external aldimine structure with aminoacrylate intermediates (see paper)
54% identity, 99% coverage: 1:378/381 of query aligns to 2:380/381 of 4iy7A

query
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4iy7A

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A

Q

Q

K

S

S

S

P

P

G

G

Q

E

H

H

K

Q

G

G

F

F

E

E

Y
|
Y

S

S

R
|
R

T
|
T

H

H

N

N

P

P

T

T

R

R

D

F

A

A

L

Y

Q

E

K

R

S

C

I

V

A

A

A

A

L

L

E

E

N

G

G

G

K

T

H

R

G

A

L

F

C

A

F

F

S

A

S

S

G
|
G

M
|
M

G

A

A

A

I

T

D

S

A

T

L

V

I

M

K

E

L

L

L

L

S

D

P

A

G

G

D

S

E

H

V

V

I

V

S

A

T

M

N

D

D

D

L

L

Y
|
Y

G

G

G

G

T

T

Y

F

R
|
R

I

L

F

F

T

E

K

R

V

V

F

R

A

R

K

R

Y

T

-

A

G

G

I

L

K

D

F

F

H

S

F

F

V

V

S

D

M

L

D

T

D

D

P

P

A

A

S

A

I

F

E

K

K

A

Y

A

I

I

N

R

D

A

K

D

T

T

R

K

L

M

I

V

W

W

A

I

E
|
E

T

T

P

P

T

T

N
|
N

P

P

M

M

M

L

N

K

I

L

I

V

D

D

I

I

K

A

A

A

L

I

A

A

A

V

I

I

A

A

G

R

K

K

H

H

D

G

L

L

L

L

L

T

G

V

V

V

D
|
D

N

N

T

T

F

F

A

A

T

S

P

P

Y

M

L

L

Q

Q

N

R

P

P

L

L

D

S

L

L

G

G

A

A

D

D

L

L

V

V

M

V

H

H

S
|
S

V

A

T

T

K
|
K

Y

Y

L

L

A

N

G

G

H

H

S

S

D

D

V

M

V

V

M

G

G

G

A

I

L

A

V

V

V

V

N

G

D

D

D

N

-

A

R

E

L

L

A

A

E

E

D

Q

L

M

A

A

F

F

I

L

Q

Q

N
|
N

S

S

C

I

G

G

A

G

T

V

P

Q

G

G

P

P

Q

F

D

D

C

S

F

F

L

L

V

A

L

L

R

R

G

G

I

L

K

K

T

T

L

L

H

P

L

L

R

R

M

M

E

R

R

A

H

H

C

C

Q

E

N

N

G

A

K

L

T

A

I

L

A

A

G

Q

Y

W

L

L

R

E

H

T

H

H

P

P

K

A

V

I

D

E

K

K

V

V

Y

I

W

Y

P

P

G

G

F

L

E

A

D

S

H

H

P

P

N

Q

H

H

D

V

I

L

A

A

A

K

K

R

Q

Q

M

M

R

S

D

G

F

F

G

G

G

G

M

I

I

V

S

S

F

I

S

V

I

L

V

K

G

G

D

G

K

F

Q

-

E

D

D

A

A

A

K

K

K

R

V

F

L

C

E

E

N

K

L

T

H

E

Y

L

F

F

S

T

L

L

A

A

E
|
E

S
|
S

L
|
L

G

G

G

G

V

V

E

E

S

S

L

L

C

V

G

N

H

H

P

P

A

A

S

V

M

M

T
|
T

H

H

A

A

S

S

I

I

P

P

K

V

V

A

E

R

R

R

E

E

K

Q

V

L

G

G

L

I

T

S

D

D

S

A

L

L

I

V

R
|
R

L

L

S

S

V

V

G

G

V

I

E

E

D

D

A

L

E

G

D

D

L

L

K

R

N

G

D

D

L

L

A

E

A

R

A

A

L

L

active site: R47 (= R46), Y99 (= Y98), D172 (= D170), K197 (= K195)
binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: G75 (= G74), M76 (= M75), Y99 (= Y98), E143 (= E141), N147 (= N145), D172 (= D170), S194 (= S192), K197 (= K195), S326 (= S324), L327 (= L325), T341 (= T339), R361 (= R359)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: Y45 (= Y44), R47 (= R46)
binding serine: Y45 (= Y44), T48 (= T47), Y99 (= Y98), R104 (= R103), N227 (= N224), E325 (= E323)

4ixzA Native structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae at ph 9.0 (see paper)
54% identity, 99% coverage: 1:378/381 of query aligns to 2:380/381 of 4ixzA

query
sites
4ixzA

M

L

K

S

F

L

G

A

T

T

K

L

V

A

I

I

H

H

A

G

G

G

V

Q

A

S

P

P

D

D

P

P

T

S

T

T

G

G

A

A

I

V

M

M

T

P

P

P

I

I

F

Y

Q

A

T

T

S

S

T

T

Y

Y

V

A

Q

Q

K

S

S

S

P

P

G

G

Q

E

H

H

K

Q

G

G

F

F

E

E

Y

Y

S

S

R
|
R

T

T

H

H

N

N

P

P

T

T

R

R

D

F

A

A

L

Y

Q

E

K

R

S

C

I

V

A

A

A

A

L

L

E

E

N

G

G

G

K

T

H

R

G

A

L

F

C

A

F

F

S

A

S

S

G

G

M

M

G

A

A

A

I

T

D

S

A

T

L

V

I

M

K

E

L

L

L

L

S

D

P

A

G

G

D

S

E

H

V

V

I

V

S

A

T

M

N

D

D

D

L

L

Y
|
Y

G

G

G

G

T

T

Y

F

R

R

I

L

F

F

T

E

K

R

V

V

F

R

A

R

K

R

Y

T

-

A

G

G

I

L

K

D

F

F

H

S

F

F

V

V

S

D

M

L

D

T

D

D

P

P

A

A

S

A

I

F

E

K

K

A

Y

A

I

I

N

R

D

A

K

D

T

T

R

K

L

M

I

V

W

W

A

I

E

E

T

T

P

P

T

T

N

N

P

P

M

M

M

L

N

K

I

L

I

V

D

D

I

I

K

A

A

A

L

I

A

A

A

V

I

I

A

A

G

R

K

K

H

H

D

G

L

L

L

L

L

T

G

V

V

V

D
|
D

N

N

T

T

F

F

A

A

T

S

P

P

Y

M

L

L

Q

Q

N

R

P

P

L

L

D

S

L

L

G

G

A

A

D

D

L

L

V

V

M

V

H

H

S

S

V

A

T

T

K
|
K

Y

Y

L

L

A

N

G

G

H

H

S

S

D

D

V

M

V

V

M

G

G

G

A

I

L

A

V

V

V

V

N

G

D

D

D

N

-

A

R

E

L

L

A

A

E

E

D

Q

L

M

A

A

F

F

I

L

Q

Q

N

N

S

S

C

I

G

G

A

G

T

V

P

Q

G

G

P

P

Q

F

D

D

C

S

F

F

L

L

V

A

L

L

R

R

G

G

I

L

K

K

T

T

L

L

H

P

L

L

R

R

M

M

E

R

R

A

H

H

C

C

Q

E

N

N

G

A

K

L

T

A

I

L

A

A

G

Q

Y

W

L

L

R

E

H

T

H

H

P

P

K

A

V

I

D

E

K

K

V

V

Y

I

W

Y

P

P

G

G

F

L

E

A

D

S

H

H

P

P

N

Q

H

H

D

V

I

L

A

A

A

K

K

R

Q

Q

M

M

R

S

D

G

F

F

G

G

G

G

M

I

I

V

S

S

F

I

S

V

I

L

V

K

G

G

D

G

K

F

Q

-

E

D

D

A

A

A

K

K

K

R

V

F

L

C

E

E

N

K

L

T

H

E

Y

L

F

F

S

T

L

L

A

A

E

E

S
|
S

L
|
L

G

G

G

G

V

V

E

E

S

S

L

L

C

V

G

N

H

H

P

P

A

A

S

V

M

M

T
|
T

H

H

A

A

S

S

I

I

P

P

K

V

V

A

E

R

R

R

E

E

K

Q

V

L

G

G

L

I

T

S

D

D

S

A

L

L

I

V

R
|
R

L

L

S

S

V

V

G

G

V

I

E

E

D

D

A

L

E

G

D

D

L

L

K

R

N

G

D

D

L

L

A

E

A

R

A

A

L

L

active site: R47 (= R46), Y99 (= Y98), D172 (= D170), K197 (= K195)
binding bicarbonate ion: Y99 (= Y98), S326 (= S324), L327 (= L325), T341 (= T339), R361 (= R359)

4iyoD Crystal structure of cystathionine gamma lyase from xanthomonas oryzae pv. Oryzae (xometc) in complex with e-site serine, a-site serine, a- site external aldimine structure with aminoacrylate and a-site iminopropionate intermediates (see paper)
54% identity, 99% coverage: 1:378/381 of query aligns to 2:380/384 of 4iyoD

query
sites
4iyoD

M

L

K

S

F

L

G

A

T

T

K

L

V

A

I

I

H

H

A

G

G

G

V

Q

A

S

P

P

D

D

P

P

T

S

T

T

G

G

A

A

I

V

M

M

T

P

P

P

I

I

F

Y

Q

A

T

T

S

S

T

T

Y

Y

V

A

Q

Q

K

S

S

S

P

P

G

G

Q

E

H

H

K

Q

G

G

F

F

E

E

Y
|
Y

S

S

R
|
R

T
|
T

H

H

N

N

P

P

T

T

R

R

D

F

A

A

L

Y

Q

E

K

R

S

C

I

V

A

A

A

A

L

L

E

E

N

G

G

G

K

T

H

R

G

A

L

F

C

A

F

F

S

A

S

S

G

G

M

M

G

A

A

A

I

T

D

S

A

T

L

V

I

M

K

E

L

L

L

L

S

D

P

A

G

G

D

S

E

H

V

V

I

V

S

A

T

M

N

D

D

D

L

L

Y
|
Y

G

G

G

G

T

T

Y

F

R
|
R

I

L

F

F

T

E

K

R

V

V

F

R

A

R

K

R

Y

T

-

A

G

G

I

L

K

D

F

F

H

S

F

F

V

V

S

D

M

L

D

T

D

D

P

P

A

A

S

A

I

F

E

K

K

A

Y

A

I

I

N

R

D

A

K

D

T

T

R

K

L

M

I

V

W

W

A

I

E

E

T

T

P

P

T

T

N

N

P

P

M

M

M

L

N

K

I

L

I

V

D

D

I

I

K

A

A

A

L

I

A

A

A

V

I

I

A

A

G

R

K

K

H

H

D

G

L

L

L

L

L

T

G

V

V

V

D
|
D

N

N

T

T

F

F

A

A

T

S

P

P

Y

M

L

L

Q

Q

N

R

P

P

L

L

D

S

L

L

G

G

A

A

D

D

L

L

V

V

M

V

H

H

S

S

V

A

T

T

K
|
K

Y

Y

L

L

A

N

G

G

H

H

S

S

D

D

V

M

V

V

M

G

G

G

A

I

L

A

V

V

V

V

N

G

D

D

D

N

-

A

R

E

L

L

A

A

E

E

D

Q

L

M

A

A

F

F

I

L

Q

Q

N
|
N

S

S

C

I

G

G

A

G

T

V

P

Q

G

G

P

P

Q

F

D

D

C

S

F

F

L

L

V

A

L

L

R

R

G

G

I

L

K

K

T

T

L

L

H

P

L

L

R

R

M

M

E

R

R

A

H

H

C

C

Q

E

N

N

G

A

K

L

T

A

I

L

A

A

G

Q

Y

W

L

L

R

E

H

T

H

H

P

P

K

A

V

I

D

E

K

K

V

V

Y

I

W

Y

P

P

G

G

F

L

E

A

D

S

H

H

P

P

N

Q

H

H

D

V

I

L

A

A

A

K

K

R

Q

Q

M

M

R

S

D

G

F

F

G

G

G

G

M

I

I

V

S

S

F

I

S

V

I

L

V

K

G

G

D

G

K

F

Q

-

E

D

D

A

A

A

K

K

K

R

V

F

L

C

E

E

N

K

L

T

H

E

Y

L

F

F

S

T

L

L

A

A

E
|
E

S
|
S

L

L

G

G

G

G

V

V

E

E

S

S

L

L

C

V

G

N

H

H

P

P

A

A

S

V

M

M

T
|
T

H

H

A

A

S

S

I

I

P

P

K

V

V

A

E

R

R

R

E

E

K

Q

V

L

G

G

L

I

T

S

D

D

S

A

L

L

I

V

R
|
R

L

L

S

S

V

V

G

G

V

I

E

E

D

D

A

L

E

G

D

D

L

L

K

R

N

G

D

D

L

L

A

E

A

R

A

A

L

L

active site: R47 (= R46), Y99 (= Y98), D172 (= D170), K197 (= K195)
binding serine: Y45 (= Y44), T48 (= T47), Y99 (= Y98), Y99 (= Y98), R104 (= R103), K197 (= K195), N227 (= N224), E325 (= E323), S326 (= S324), T341 (= T339), R361 (= R359)

6k1lB E53a mutant of a putative cystathionine gamma-lyase (see paper)
54% identity, 99% coverage: 1:379/381 of query aligns to 3:382/382 of 6k1lB

query
sites
6k1lB

M

L

K

A

F

L

G

A

T

T

K

L

V

A

I

I

H

H

A

G

G

G

V

Q

A

S

P

P

D

D

P

P

T

S

T

T

G

G

A

A

I

V

M

M

T

P

P

P

I

I

F

Y

Q

A

T

T

S

S

T

T

Y

Y

V

A

Q

Q

K

S

S

S

P

P

G

G

Q

E

H

H

K

Q

G

G

F

F

E

A

Y
|
Y

S

S

R
|
R

T

T

H

H

N

N

P

P

T

T

R

R

D

F

A

A

L

Y

Q

E

K

R

S

C

I

V

A

A

A

S

L

L

E

E

N

G

G

G

K

T

H

R

G

G

L

F

C

A

F

F

S

A

S
|
S

G
|
G

M
|
M

G

A

A

A

I

S

D

S

A

T

L

V

I

I

K

E

L

L

L

L

S

D

P

A

G

G

D

S

E

H

V

V

I

V

S

A

T

M

N

D

D

D

L

I

Y
|
Y

G

G

G

G

T

S

Y

F

R

R

I

L

F

F

T

E

K

R

V

V

F

R

A

R

K

R

Y

T

-

A

G

G

I

L

K

D

F

F

H

S

F

F

V

V

S

D

M

L

D

T

D

D

P

L

A

A

S

A

I

F

E

E

K

A

Y

S

I

I

N

T

D

P

K

K

T

T

R

K

L

M

I

V

W

W

A

I

E

E

T

T

P

P

T

T

N

N

P

P

M

M

M

L

N

K

I

I

I

V

D

D

I

I

K

A

A

A

L

V

A

A

A

A

I

I

A

A

G

K

K

R

H

H

D

G

L

L

L

I

L

V

G

V

V

V

D
|
D

N

N

T

T

F

F

A

A

T

S

P

P

Y

M

L

L

Q

Q

N

R

P

P

L

L

D

E

L

L

G

G

A

A

D

D

L

L

V

V

M

L

H

H

S
|
S

V

A

T

T

K
|
K

Y

Y

L

L

A

N

G

G

H

H

S

S

D

D

V

M

V

V

M

G

G

G

A

M

L

V

V

V

V

V

N

G

D

D

D

N

-

A

R

E

L

L

A

A

E

E

D

Q

L

M

A

A

F

F

I

L

Q

Q

N

N

S

S

C

V

G

G

A

G

T

V

P

Q

G

G

P

P

Q

F

D

D

C

S

F

F

L

L

V

A

L

L

R

R

G

G

I

L

K

K

T

T

L

L

H

P

L

L

R

R

M

M

E

K

R

A

H

H

C

C

Q

A

N

N

G

A

K

L

T

A

I

L

A

A

G

Q

Y

W

L

L

R

E

H

K

H

H

P

P

K

A

V

V

D

E

K

K

V

V

Y

I

W

Y

P

P

G

G

F

L

E

A

D

S

H

H

P

P

N

Q

H

H

D

E

I

L

A

A

A

G

K

K

Q

Q

M

M

R

A

D

G

F

Y

G

G

G

G

M

I

I

V

S

S

F

I

S

V

I

L

V

K

G

G

D

G

K

F

Q

-

E

D

D

A

A

A

K

K

K

R

V

F

L

C

E

E

N

K

L

T

H

E

Y

L

F

F

S

T

L

L

A

A

E
|
E

S

S

L

L

G

G

G

G

V

V

E

E

S

S

L

L

C

V

G

N

H

H

P

P

A

A

S

V

M

M

T
|
T

H

H

A

A

S

S

I

I

P

P

K

V

V

A

E

R

R

R

E

E

K

Q

V

L

G

G

L

I

T

S

D

D

S

A

L

L

I

V

R
|
R

L

L

S

S

V

V

G

G

V

V

E

E

D

D

A

L

E

G

D

D

L

L

K

Q

N

V

D

D

L

L

A

G

A

E

A

A

L

L

E

K

binding pyridoxal-5'-phosphate: Y46 (= Y44), R48 (= R46), S75 (= S73), G76 (= G74), M77 (= M75), Y100 (= Y98), D173 (= D170), S195 (= S192), K198 (= K195)
binding pyruvic acid: Y100 (= Y98), K198 (= K195), E326 (= E323), T342 (= T339), R362 (= R359)

6k1lA E53a mutant of a putative cystathionine gamma-lyase (see paper)
54% identity, 99% coverage: 1:379/381 of query aligns to 3:382/382 of 6k1lA

query
sites
6k1lA

M

L

K

A

F

L

G

A

T

T

K

L

V

A

I

I

H

H

A

G

G

G

V

Q

A

S

P

P

D

D

P

P

T

S

T

T

G

G

A

A

I

V

M

M

T

P

P

P

I

I

F

Y

Q

A

T

T

S

S

T

T

Y

Y

V

A

Q

Q

K

S

S

S

P

P

G

G

Q

E

H

H

K

Q

G

G

F

F

E

A

Y
|
Y

S

S

R
|
R

T

T

H

H

N

N

P

P

T

T

R

R

D

F

A

A

L

Y

Q

E

K

R

S

C

I

V

A

A

A

S

L

L

E

E

N

G

G

G

K

T

H

R

G

G

L

F

C

A

F

F

S

A

S

S

G
|
G

M
|
M

G

A

A

A

I

S

D

S

A

T

L

V

I

I

K

E

L

L

L

L

S

D

P

A

G

G

D

S

E

H

V

V

I

V

S

A

T

M

N

D

D

D

L

I

Y
|
Y

G

G

G

G

T

S

Y

F

R

R

I

L

F

F

T

E

K

R

V

V

F

R

A

R

K

R

Y

T

-

A

G

G

I

L

K

D

F

F

H

S

F

F

V

V

S

D

M

L

D

T

D

D

P

L

A

A

S

A

I

F

E

E

K

A

Y

S

I

I

N

T

D

P

K

K

T

T

R

K

L

M

I

V

W

W

A

I

E

E

T

T

P

P

T

T

N

N

P

P

M

M

M

L

N

K

I

I

I

V

D

D

I

I

K

A

A

A

L

V

A

A

A

A

I

I

A

A

G

K

K

R

H

H

D

G

L

L

L

I

L

V

G

V

V

V

D
|
D

N

N

T

T

F

F

A

A

T

S

P

P

Y

M

L

L

Q

Q

N

R

P

P

L

L

D

E

L

L

G

G

A

A

D

D

L

L

V

V

M

L

H

H

S
|
S

V

A

T
|
T

K
|
K

Y

Y

L

L

A

N

G

G

H

H

S

S

D

D

V

M

V

V

M

G

G

G

A

M

L

V

V

V

V

V

N

G

D

D

D

N

-

A

R

E

L

L

A

A

E

E

D

Q

L

M

A

A

F

F

I

L

Q

Q

N

N

S

S

C

V

G

G

A

G

T

V

P

Q

G

G

P

P

Q

F

D

D

C

S

F

F

L

L

V

A

L

L

R

R

G

G

I

L

K

K

T

T

L

L

H

P

L

L

R

R

M

M

E

K

R

A

H

H

C

C

Q

A

N

N

G

A

K

L

T

A

I

L

A

A

G

Q

Y

W

L

L

R

E

H

K

H

H

P

P

K

A

V

V

D

E

K

K

V

V

Y

I

W

Y

P

P

G

G

F

L

E

A

D

S

H

H

P

P

N

Q

H

H

D

E

I

L

A

A

A

G

K

K

Q

Q

M

M

R

A

D

G

F

Y

G

G

G

G

M

I

I

V

S

S

F

I

S

V

I

L

V

K

G

G

D

G

K

F

Q

-

E

D

D

A

A

A

K

K

K

R

V

F

L

C

E

E

N

K

L

T

H

E

Y

L

F

F

S

T

L

L

A

A

E

E

S

S

L

L

G

G

G

G

V

V

E

E

S

S

L

L

C

V

G

N

H

H

P

P

A

A

S

V

M

M

T

T

H

H

A

A

S

S

I

I

P

P

K

V

V

A

E

R

R

R

E

E

K

Q

V

L

G

G

L

I

T

S

D

D

S

A

L

L

I

V

R

R

L

L

S

S

V

V

G

G

V

V

E

E

D

D

A

L

E

G

D

D

L

L

K

Q

N

V

D

D

L

L

A

G

A

E

A

A

L

L

E

K

binding pyridoxal-5'-phosphate: Y46 (= Y44), R48 (= R46), G76 (= G74), M77 (= M75), Y100 (= Y98), D173 (= D170), S195 (= S192), T197 (= T194), K198 (= K195)

6cjaA Crystal structure of cystathionine beta-lyase from legionella pneumophila philadelphia 1 in complex with alanyl-plp and serine
54% identity, 99% coverage: 3:378/381 of query aligns to 4:380/381 of 6cjaA

query
sites
6cjaA

F

F

G

D

T

T

K

R

V

A

I

I

H

H

A

A

G

G

V

Q

A

E

P

P

D

C

P

K

T

S

T

T

G

G

A

A

I

V

M

M

T

T

P

P

I

I

F

Y

Q

A

T

T

S

S

T

T

Y

Y

V

K

Q

Q

K

I

S

A

P

P

G

G

Q

E

H

H

K

L

G

G

F

Y

E
|
E

Y
|
Y

S

S

R
|
R

T
|
T

H

Q

N

N

P

P

T

T

R

R

D

K

A

A

L

Y

Q

E

K

D

S

C

I

I

A

A

A

S

L

L

E

E

N

S

G

G

K

Q

H

K

G

G

L

F

C

A

F

F

S

A

S
|
S

G
|
G

M
|
M

G

A

A

A

I

I

D

N

A

T

L

V

I

I

K

D

L

L

L

L

S

G

P

S

G

G

D

D

E

H

V

V

I

V

S

A

T

M

N

D

D

D

L

L

Y
|
Y

G

G

G

G

T

T

Y

F

R
|
R

I

L

F

F

T

D

K

K

V

V

F

K

A

T

K

R

Y

T

G

S

-

N

I

L

K

S

F

F

H

S

F

F

V

I

S

D

M

M

D

S

D

V

P

P

A

E

S

N

I

I

E

E

K

A

Y

A

I

I

N

T

D

P

K

K

T

T

R

K

L

L

I

L

W

W

A

L

E

E

T

T

P

P

T

S

N
|
N

P

P

M

M

M

L

N

K

I

L

I

A

D

N

I

L

K

R

A

K

L

I

A

A

A

A

I

I

A

A

G

K

K

K

H

Y

D

N

L

L

L

I

L

T

G

V

V

A

D
|
D

N

N

T

T

F

F

A

A

T

T

P

P

Y

W

L

I

Q

Q

N

R

P

P

L

L

D

E

L

L

G

G

A

F

D

D

L

I

V

V

M

L

H

H

S
|
S

V

A

T
|
T

K
|
K

Y

Y

L

L

A

N

G

G

H

H

S

S

D

D

V

V

V

V

M

S

G

G

A

V

L

V

V

V

V

V

N

G

D

D

D

N

R

S

-

V

L

L

A

S

E

D

D

K

L

I

A

A

F

F

I

L

Q

Q

N

N

S

S

C

C

G

G

A

A

T

V

P

A

G

G

P

P

Q

F

D

D

C

S

F

F

L

L

V

V

L

L

R

R

G

S

I

L

K

K

T

T

L

L

H

S

L

V

R

R

M

M

E

Q

R

R

H

H

C

C

Q

E

N

N

G

A

K

N

T

H

I

L

A

A

G

N

Y

W

L

L

R

S

H

S

H

H

P

P

K

K

V

I

D

E

K

K

V

V

Y

I

W

Y

P

P

G

G

F

L

E

K

D

S

H

H

P

P

N

Q

H

Y

D

S

I

L

A

A

A

K

K

E

Q

Q

M

M

R

N

D

N

F

F

G

G

G

G

M

M

I

I

S

S

F

L

S

V

I

L

V

K

G

G

D

S

K

-

Q

L

E

E

D

D

A

A

K

K

K

R

V

F

L

L

E

A

N

R

L

C

H

E

Y

L

F

F

S

T

L

L

A

A

E
|
E

S
|
S

L

L

G

G

G

G

V

V

E

E

S

S

L

L

C

I

G

E

H

H

P

P

A

A

S

I

M

M

T
|
T

H

H

A

A

S

S

I

I

P

P

K

V

V

E

E

Q

R

R

E

K

K

A

V

L

G

G

L

I

T

E

D

D

S

G

L

F

I

I

R
|
R

L

L

S

S

V

V

G

G

V

I

E

E

D

H

A

I

E

D

D

D

L

L

K

R

N

A

D

D

L

L

A

E

A

H

A

A

L

L

binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-alanine: Y45 (= Y44), R47 (= R46), S74 (= S73), G75 (= G74), M76 (= M75), Y99 (= Y98), N147 (= N145), D172 (= D170), S194 (= S192), T196 (= T194), K197 (= K195), S326 (= S324), T341 (= T339), R361 (= R359)
binding serine: E44 (= E43), R47 (= R46), T48 (= T47), R104 (= R103), E325 (= E323)

4ixsB Native structure of xometc at ph 5.2 (see paper)
52% identity, 99% coverage: 1:378/381 of query aligns to 1:371/372 of 4ixsB

query
sites
4ixsB

M

L

K

S

F

L

G

A

T

T

K

L

V

A

I

I

H

H

A

G

G

G

V

Q

A

S

P

P

D

D

P

P

T

S

T

T

G

G

A

A

I

V

M

M

T

P

P

P

I

I

F

Y

Q

A

T

T

S

S

T

T

Y

Y

V

A

Q

Q

K

S

S

S

P

P

G

-

Q

-

H

-

K

-

G

-

F

-

E

-

Y

-

S

S

R
|
R

T

T

H

H

N

N

P

P

T

T

R

R

D

F

A

A

L

Y

Q

E

K

R

S

C

I

V

A

A

A

A

L

L

E

E

N

G

G

G

K

T

H

R

G

A

L

F

C

A

F

F

S

A

S

S

G

G

M

M

G

A

A

A

I

T

D

S

A

T

L

V

I

M

K

E

L

L

L

L

S

D

P

A

G

G

D

S

E

H

V

V

I

V

S

A

T

M

N

D

D

D

L

L

Y
|
Y

G

G

G

G

T

T

Y

F

R

R

I

L

F

F

T

E

K

R

V

V

F

R

A

R

K

R

Y

T

-

A

G

G

I

L

K

D

F

F

H

S

F

F

V

V

S

D

M

L

D

T

D

D

P

P

A

A

S

A

I

F

E

K

K

A

Y

A

I

I

N

R

D

A

K

D

T

T

R

K

L

M

I

V

W

W

A

I

E

E

T

T

P

P

T

T

N

N

P

P

M

M

M

L

N

K

I

L

I

V

D

D

I

I

K

A

A

A

L

I

A

A

A

V

I

I

A

A

G

R

K

K

H

H

D

G

L

L

L

L

L

T

G

V

V

V

D
|
D

N

N

T

T

F

F

A

A

T

S

P

P

Y

M

L

L

Q

Q

N

R

P

P

L

L

D

S

L

L

G

G

A

A

D

D

L

L

V

V

M

V

H

H

S

S

V

A

T

T

K
|
K

Y

Y

L

L

A

N

G

G

H

H

S

S

D

D

V

M

V

V

M

G

G

G

A

I

L

A

V

V

V

V

N

G

D

D

D

N

-

A

R

E

L

L

A

A

E

E

D

Q

L

M

A

A

F

F

I

L

Q

Q

N

N

S

S

C

I

G

G

A

G

T

V

P

Q

G

G

P

P

Q

F

D

D

C

S

F

F

L

L

V

A

L

L

R

R

G

G

I

L

K

K

T

T

L

L

H

P

L

L

R

R

M

M

E

R

R

A

H

H

C

C

Q

E

N

N

G

A

K

L

T

A

I

L

A

A

G

Q

Y

W

L

L

R

E

H

T

H

H

P

P

K

A

V

I

D

E

K

K

V

V

Y

I

W

Y

P

P

G

G

F

L

E

A

D

S

H

H

P

P

N

Q

H

H

D

V

I

L

A

A

A

K

K

R

Q

Q

M

M

R

S

D

G

F

F

G

G

G

G

M

I

I

V

S

S

F

I

S

V

I

L

V

K

G

G

D

G

K

F

Q

-

E

D

D

A

A

A

K

K

K

R

V

F

L

C

E

E

N

K

L

T

H

E

Y

L

F

F

S

T

L

L

A

A

E
|
E

S
|
S

L

L

G

G

G

G

V

V

E

E

S

S

L

L

C

V

G

N

H

H

P

P

A

A

S

V

M

M

T
|
T

H

H

A

A

S

S

I

I

P

P

K

V

V

A

E

R

R

R

E

E

K

Q

V

L

G

G

L

I

T

S

D

D

S

A

L

L

I

V

R
|
R

L

L

S

S

V

V

G

G

V

I

E

E

D

D

A

L

E

G

D

D

L

L

K

R

N

G

D

D

L

L

A

E

A

R

A

A

L

L

active site: R38 (= R46), Y90 (= Y98), D163 (= D170), K188 (= K195)
binding carbonate ion: K188 (= K195), E316 (= E323), S317 (= S324), T332 (= T339), R352 (= R359)

7ba4A Structure of cystathionine gamma-lyase from pseudomonas aeruginosa
52% identity, 99% coverage: 3:381/381 of query aligns to 7:374/377 of 7ba4A

query
sites
7ba4A

F

F

G

A

T

T

K

R

V

V

I

I

H

H

A

A

G

G

V

Q

A

A

P

P

D

D

P

P

T

S

T

T

G

G

A

A

I

I

M

M

T

P

P

P

I

I

F

Y

Q

A

T

N

S

S

T

T

Y

Y

V

I

Q

Q

K

-

S

-

P

-

G

-

Q

-

H

-

K

-

G

-

F

-

E

-

Y

-

S

-

R

R

T

S

H

H

N

N

P

P

T

T

R

R

D

W

A

A

L

L

Q

E

K

R

S

C

I

V

A

A

A

D

L

L

E

E

N

G

G

G

K

T

H

Q

G

A

L

F

C

A

F

F

S

A

S

S

G
|
G

M
x
L

G

A

A

A

I

I

D

S

A

S

L

V

I

L

K

E

L

L

L

L

S

D

P

A

G

G

D

S

E

H

V

I

I

V

S

S

T

G

N

N

D

D

L

L

Y
|
Y

G

G

G

G

T

T

Y

F

R

R

I

L

F

F

T

E

K

R

V

V

F

R

A

R

K

R

Y

S

-

A

G

G

I

H

K

R

F

F

H

S

F

F

V

V

S

D

M

P

D

T

D

D

P

L

A

Q

S

A

I

F

E

E

K

A

Y

A

I

L

N

T

D

P

K

E

T

T

R

R

L

M

I

V

W

W

A

V

E

E

T

T

P

P

T

S

N

N

P

P

M

L

M

L

N

R

I

L

I

T

D

D

I

L

K

R

A

A

L

I

A

A

A

Q

I

L

A

C

G

R

K

A

H

R

D

G

L

I

L

I

L

S

G

V

V

A

D
|
D

N

N

T

T

F
|
F

A

A

T

S

P

P

Y

Y

L

I

Q

Q

N

R

P

P

L

L

D

E

L

L

G

G

A

F

D

D

L

V

V

V

M

V

H

H

S
|
S

V

T

T
|
T

K
|
K

Y

Y

L

L

A

N

G

G

H

H

S

S

D

D

V

V

V

I

M

G

G

G

A

I

L

A

V

I

V

V

N

G

D

D

D

N

-

P

R

D

L

L

A

R

E

E

D

R

L

L

A

G

F

F

I

L

Q

Q

N

N

S

S

C

V

G

G

A

A

T

I

P

S

G

G

P

P

Q

F

D

D

C

A

F

F

L

L

V

T

L

L

R

R

G

G

I

V

K

K

T

T

L

L

H

A

L

L

R

R

M

M

E

E

R

R

H

H

C

C

Q

S

N

N

G

A

K

L

T

A

I

L

A

A

G

Q

Y

W

L

L

R

E

H

R

H

Q

P

P

K

Q

V

V

D

A

K

R

V

V

Y

Y

W

Y

P

P

G

G

F

L

E

A

D

S

H

H

P

P

N

Q

H

H

D

E

I

L

A

A

A

K

K

R

Q

Q

M

M

R

R

D

G

F

F

G

G

G

G

M

M

I

I

S

S

F

L

S

D

I

L

V

R

G

C

D

D

K

L

Q

-

E

A

D

G

A

A

K

R

K

R

V

F

L

L

E

E

N

N

L

V

H

R

Y

I

F

F

S

S

L

L

A

A

E

E

S

S

L

L

G

G

G

G

V

V

E

E

S

S

L

L

C

I

G

E

H

H

P

P

A

A

S

I

M

M

T

T

H

H

A

A

S

S

I

I

P

P

K

A

V

E

E

T

R

R

E

A

K

D

V

L

G

G

L

I

T

G

D

D

S

S

L

L

I

I

R

R

L

L

S

S

V

V

G

G

V

V

E

E

D

A

A

L

E

E

D

D

L

L

K

Q

N

A

D

D

L

L

A

A

A

Q

A

A

L

L

E

A

L

K

I

I

binding pyridoxal-5'-phosphate: G66 (= G74), L67 (≠ M75), Y90 (= Y98), D163 (= D170), F166 (= F173), S185 (= S192), T187 (= T194), K188 (= K195)

7mcyH Crystal structure of staphylococcus aureus cystathionine gamma lyase, holoenzyme with bound nl3 (see paper)
52% identity, 99% coverage: 5:381/381 of query aligns to 5:380/380 of 7mcyH

query
sites
7mcyH

T

T

K
|
K

V

L

I

I

H
|
H

A
x
G

G

G

V

H

A

T

P

T

D

D

P

D

T

Y

T

T

G

G

A

A

I

V

M

T

T

T

P

P

I

I

F

Y

Q

Q

T

T

S

S

T

T

Y

Y

V

L

Q

Q

K

D

S

D

P

I

G

G

Q

D

-

L

H

R

K

Q

G

G

F

Y

E

E

Y

Y

S

S

R

R

T

T

H

A

N

N

P

P

T

T

R

R

D

S

A

S

L

V

Q

E

K

S

S

V

I

I

A

A

A

T

L

L

E

E

N

N

G

G

K

K

H

H

G

G

L

F

C

A

F

F

S

S

S

S

G

G

M

V

G

A

A

A

I

I

D

S

A

A

L

V

I

V

K

M

L

L

L

L

S

D

P

K

G

G

D

D

E

H

V

I

I

I

S

L

T

N

N

S

D
|
D

L
x
V

Y
|
Y

G
|
G

G

G

T

T

Y
|
Y

R

R

I

A

F

L

T

T

K

K

V

V

F

F

A

T

K

R

Y

F

G

G

I

I

K

E

F

V

H

D

F

F

V

V

S

D

M

T

D

T

D

H

P

T

A

D

S

S

I

I

E

V

K

Q

Y

A

I

I

N

R

D

P

K

T

T

T

R

K

L

M

I

L

W

F

A

I

E

E

T

T

P

P

T

S

N
|
N

P
|
P

M

L

M

L

N

R

I

V

I

T

D

D

I

I

K

K

A

K

L

S

A

A

A

E

I

I

A

A

G

K

K

E

H

H

D

G

L

L

L

I

L

S

G

V

V

V

D

D

N

N

T

T

F

F

A

M

T

T

P

P

Y

Y

L

Y

Q

Q

N

N

P

P

L

L

D

D

L

L

G

G

A

I

D

D

L

I

V

V

M

L

H

H

S

S

V

A

T

T

K

K

Y

Y

L

L

A

G

G

G

H

H

S

S

D

D

V

V

V

V

M

A

G

G

A

L

L

V

V

A

V

T

N

S

D

D

D

D

R

K

L

L

A

A

E

E

D

R

L

L

A

A

F

F

I

I

Q

S

N

N

S

S

C

T

G

G

A

G

T

I

P

L

G

G

P

P

Q

Q

D

D

C

S

F

Y

L

L

V

L

L

V

R

R

G

G

I

I

K

K

T

T

L

L

H

G

L

L

R

R

M

M

E

E

R

Q

H

I

C

N

Q

R

N

S

G

V

K

I

T

E

I

I

A

I

G

K

Y

M

L

L

R

Q

H

A

H

H

P

P

K

A

V

V

D

Q