SitesBLAST

Q2I8V6 1,5-anhydro-D-fructose reductase; Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming); EC 1.1.1.292 from Ensifer adhaerens (Sinorhizobium morelense) (see paper)
30% identity, 31% coverage: 62:213/486 of query aligns to 18:170/333 of Q2I8V6

query
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Q2I8V6

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S33 (≠ C77) mutation to D: No activity.
ST 33:34 (≠ CD 77:78) binding
R38 (= R82) binding
TTNELH 71:76 (≠ TPDHNH 118:123) binding
EK 93:94 (≠ QK 140:141) binding
K94 (= K141) mutation to G: Less than 1% remaining activity.
N120 (≠ G167) binding
WR 162:163 (vs. gap) binding

Sites not aligning to the query:

9:12 binding
10 S→G: Almost no effect.
13 A→G: Can use NAD as cosubstrate as well as NADP.
176 D→A: Less than 1% remaining activity.
180 H→A: Less than 2% remaining activity.
206 G→I: No effect.
283 binding

2glxA Crystal structure analysis of bacterial 1,5-af reductase (see paper)
30% identity, 31% coverage: 62:213/486 of query aligns to 17:169/332 of 2glxA

query
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2glxA

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active site: K93 (= K141)
binding acetate ion: K93 (= K141)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: S32 (≠ C77), T33 (≠ D78), R37 (= R82), S69 (= S117), T70 (= T118), N72 (≠ D120), H75 (= H123), E92 (≠ Q140), K93 (= K141), H121 (≠ Q169), W161 (vs. gap), R162 (vs. gap)

Sites not aligning to the query:

active site: 179
binding acetate ion: 179
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 7, 8, 9, 10, 11, 282

4n54A Crystal structure of scyllo-inositol dehydrogenase from lactobacillus casei with bound cofactor NAD(h) and scyllo-inositol
27% identity, 32% coverage: 46:201/486 of query aligns to 4:157/340 of 4n54A

query
sites
4n54A

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active site: K96 (= K141)
binding (1r,2r,3r,4r,5r,6r)-cyclohexane-1,2,3,4,5,6-hexol: R12 (≠ A54), K96 (= K141), D156 (≠ R200)
binding 1,4-dihydronicotinamide adenine dinucleotide: V8 (≠ A50), G9 (= G51), L10 (≠ I52), G11 (= G53), R12 (≠ A54), L13 (≠ G55), S35 (≠ D78), V36 (= V79), E40 (≠ R83), S73 (≠ T118), P74 (= P119), F77 (≠ N122), H78 (= H123), E95 (≠ Q140), K96 (= K141), M125 (≠ Q169)

Sites not aligning to the query:

active site: 183
binding (1r,2r,3r,4r,5r,6r)-cyclohexane-1,2,3,4,5,6-hexol: 179, 180, 183, 238, 244
binding 1,4-dihydronicotinamide adenine dinucleotide: 167, 284

3e18A Crystal structure of NAD-binding protein from listeria innocua
28% identity, 19% coverage: 52:144/486 of query aligns to 9:97/348 of 3e18A

query
sites
3e18A

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active site: K94 (= K141)
binding nicotinamide-adenine-dinucleotide: G12 (= G55), G13 (= G56), M14 (≠ K57), F34 (≠ C77), D35 (= D78), I36 (≠ V79), K40 (≠ R82), A70 (≠ S117), T71 (= T118), P72 (= P119), N73 (≠ D120), H76 (= H123), E93 (≠ Q140), K94 (= K141)

Sites not aligning to the query:

active site: 178
binding nicotinamide-adenine-dinucleotide: 122, 161, 162, 178

3nt5A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor and product inosose (see paper)
30% identity, 31% coverage: 49:201/486 of query aligns to 5:158/337 of 3nt5A

query
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3nt5A

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active site: K97 (= K141)
binding (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone: K97 (= K141), H155 (≠ T198)
binding nicotinamide-adenine-dinucleotide: G9 (= G53), G11 (= G55), A12 (≠ G56), I13 (≠ K57), D35 (= D78), V36 (= V79), S74 (≠ T118), W75 (vs. gap), G76 (vs. gap), E96 (≠ Q140), K97 (= K141)

Sites not aligning to the query:

3nt4A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor nadh and inositol (see paper)
30% identity, 31% coverage: 49:201/486 of query aligns to 5:158/337 of 3nt4A

query
sites
3nt4A

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active site: K97 (= K141)
binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: H155 (≠ T198)
binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G55), A12 (≠ G56), I13 (≠ K57), D35 (= D78), V36 (= V79), S74 (≠ T118), W75 (vs. gap), G76 (vs. gap), E96 (≠ Q140), K97 (= K141)

Sites not aligning to the query:

active site: 176
binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: 176, 235, 272
binding 1,4-dihydronicotinamide adenine dinucleotide: 176

3nt2B Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor (see paper)
30% identity, 31% coverage: 49:201/486 of query aligns to 5:158/337 of 3nt2B

query
sites
3nt2B

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P

P

active site: K97 (= K141)
binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G53), G11 (= G55), A12 (≠ G56), D35 (= D78), V36 (= V79), S74 (≠ T118), W75 (vs. gap), A78 (≠ D120), K97 (= K141)

Sites not aligning to the query:

active site: 176
binding 1,4-dihydronicotinamide adenine dinucleotide: 272, 280

3nt2A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor (see paper)
30% identity, 31% coverage: 49:201/486 of query aligns to 5:158/337 of 3nt2A

query
sites
3nt2A

I

I

A

G

G

V

I

I

G
|
G

A

T

G
|
G

G
x
A

K
x
I

G

G

Q

K

S

E

D

H

I

I

N

N

A

R

M

I

Y

T

Q

N

S

K

-

L

G

S

H

G

A

A

E

E

I

I

G

V

Y

A

L

V

C

T

D
|
D

V
|
V

D

N

D

Q

R

E

R

A

A

A

A

Q

T

K

S

V

R

V

E

E

R

Q

F

Y

P

Q

-

L

K

N

A

A

K

T

Y

V

Y

Y

K

P

D

N

F

D

R

D

E

S

L

L

E

A

K

D

E

E

H

-

K

-

N

N

I

V

D

D

A

A

V

V

T

L

V

V

S

T

T
x
S

-
x
W

-
x
G

P

P

D
x
A

H
|
H

N

E

H

S

A

S

V

V

I

-

T

-

L

L

A

K

A

A

M

I

Q

K

L

A

G

Q

K

K

H

Y

V

V

Y

F

V

C

Q
x
E

K
|
K

P

P

L

L

T

A

H

T

D

T

I

A

Y

E

E

G

A

C

-

M

R

R

M

I

L

V

T

E

E

E

A

E

A

I

E

K

K

V

Y

G

K

K

V

R

V

L

T

V

Q

Q

M

V

G

G

N

F

Q

M

G

R

S

R

S

Y

G

D

D

S

G

G

V

Y

R

V

R

Q

M

L

V

K

E

E

W

A

Y

L

D

D

A

N

G

H

L

V

I

I

G

G

E

E

A

P

T

L

K

M

V

I

W

H

C

C

W

A

T

H

D

R

R

N

P

P

active site: K97 (= K141)
binding nicotinamide-adenine-dinucleotide: G9 (= G53), G11 (= G55), A12 (≠ G56), I13 (≠ K57), D35 (= D78), V36 (= V79), S74 (≠ T118), W75 (vs. gap), G76 (vs. gap), A78 (≠ D120), H79 (= H121), E96 (≠ Q140), K97 (= K141)

Sites not aligning to the query:

active site: 176
binding nicotinamide-adenine-dinucleotide: 176, 280

Query Sequence

>Echvi_2281 FitnessBrowser__Cola:Echvi_2281
MKEQKPLQSGNSRRAFLKGAATAAAGFYLVPRHVLGGPGFTAPSDKIVIAGIGAGGKGQS
DINAMYQSGHAEIGYLCDVDDRRAATSRERFPKAKYYKDFRELLEKEHKNIDAVTVSTPD
HNHAVITLAAMQLGKHVYVQKPLTHDIYEARMLTEAAEKYKVVTQMGNQGSSGDGVRRMV
EWYDAGLIGEATKVWCWTDRPVWPQGIKWPEKGTTPPKELDWDLWLGTAPYKEYVDNLVP
FNWRGWWDYGTGALGDMACHIMEPPFRALGLGYPTEAECSVGSVYVGEFQRGYFPDGCPP
SSHVTLKFKMPSGKDLEFHWMDGGIQPTRPEELGPNEQMGDGGNGVIIEGTKGKMMCSTY
GINPMLLPTSREDGEKVPKTIPRVENGDNGHYAQWVKACVAGHGSKEFKELSSPFSIAGP
LTESVLMGNLAIRSYDIRKPREDNGFDYPGRGIKLVWDGPNMKVTNFDEANQFVKREYRG
DWTLNV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory