SitesBLAST

P00512 ATP-dependent 6-phosphofructokinase; ATP-PFK; Phosphofructokinase; Phosphohexokinase; EC 2.7.1.11 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see 2 papers)
51% identity, 100% coverage: 1:324/324 of query aligns to 1:319/319 of P00512

query
sites
P00512

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RSVVR 21:25 (≠ RAVVR 21:25) binding
D59 (≠ N59) binding
RC 72:73 (≠ RS 72:73) binding
D103 (= D103) binding
TID 125:127 (= TID 126:128) binding in other chain
R154 (= R155) binding in other chain
R162 (= R163) binding
MGR 169:171 (= MGR 170:172) binding in other chain
GAE 185:187 (= GAE 186:188) binding in other chain
R211 (≠ K211) binding in other chain
KKH 213:215 (≠ KTS 213:215) binding in other chain
E222 (= E222) binding in other chain
R243 (≠ K248) binding
HVQR 249:252 (≠ HIQR 254:257) binding in other chain

1mtoA Crystal structure of a phosphofructokinase mutant from bacillus stearothermophilus bound with fructose-6-phosphate (see paper)
51% identity, 100% coverage: 1:324/324 of query aligns to 1:319/319 of 1mtoA

query
sites
1mtoA

M

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active site: G11 (= G11), R72 (= R72), C73 (≠ S73), D103 (= D103), G104 (= G104), G124 (= G125), T125 (= T126), D127 (= D128), D129 (= D130), R171 (= R172)
binding 6-O-phosphono-beta-D-fructofuranose: D127 (= D128), R162 (= R163), M169 (= M170), R171 (= R172), E222 (= E222), R243 (≠ K248), H249 (= H254), R252 (= R257)

4pfkA Phosphofructokinase. Structure and control (see paper)
50% identity, 100% coverage: 1:324/324 of query aligns to 1:319/319 of 4pfkA

query
sites
4pfkA

M

M

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active site: G11 (= G11), R72 (= R72), C73 (≠ S73), D103 (= D103), G104 (= G104), G124 (= G125), T125 (= T126), D127 (= D128), D129 (= D130), R171 (= R172)
binding adenosine-5'-diphosphate: S9 (= S9), Y41 (= Y41), R72 (= R72), C73 (≠ S73), F76 (= F76), K77 (≠ R77), G104 (= G104), G108 (= G108), R154 (= R155), G185 (= G186), R211 (≠ K211), G212 (≠ T212), K213 (= K213), H215 (≠ S215)
binding 6-O-phosphono-beta-D-fructofuranose: D127 (= D128), M169 (= M170), E222 (= E222), H249 (= H254), R252 (= R257)
binding magnesium ion: G185 (= G186), E187 (= E188)

3pfkA Phosphofructokinase. Structure and control (see paper)
50% identity, 100% coverage: 1:324/324 of query aligns to 1:319/319 of 3pfkA

query
sites
3pfkA

M

M

K

K

K

R

I

I

A

G

V

V

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L

T

T

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S

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G

G
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D

D

A

S

P

P

G

G

M

M

N

N

A

A

C

A

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C

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A

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V

G

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T

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D

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N

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D

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I

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P

G

G

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T

D

D

F

F

T

T

I

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A

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A

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D

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A

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S

S

V

I

I

I

V

I

V

V

A

A

E

E

G

G

D

-

E

-

E

V

G

G

S

S

A

G

A

V

E

D

I

F

M

G

E

R

K

Q

V

I

K

Q

N

E

I

A

I

T

N

G

D

F

D

E

T

T

K

-

D

-

F

-

K

R

V

V

T

T

T

V

L

L

G

G

H
|
H

I

V

Q

Q

R
|
R

G

G

N

S

P

P

T

T

G

A

K

F

D

D

R

R

M

V

L

L

A

A

S

S

R

R

C

L

G

G

M

A

A

R

A

A

V

V

E

E

G

L

L

L

M

L

N

E

G

G

Q

K

A

G

N

G

C

R

M

C

A

V

G

G

I

I

I

Q

H

N

D

N

E

Q

V

L

V

V

Y

D

T

H

S

D

F

I

E

A

D

E

C

A

I

L

T

A

K

N

S

K

K

H

P

T

L

I

N

D

K

Q

D

R

T

M

L

Y

K

A

L

L

I

S

E

K

I

E

L

L

S

S

I

I

active site: G11 (= G11), R72 (= R72), C73 (≠ S73), D103 (= D103), G104 (= G104), G124 (= G125), T125 (= T126), D127 (= D128), D129 (= D130), R171 (= R172)
binding phosphate ion: R154 (= R155), K213 (= K213), H249 (= H254), R252 (= R257)

4i4iA Crystal structure of bacillus stearothermophilus phosphofructokinase mutant t156a bound to pep (see paper)
50% identity, 100% coverage: 1:324/324 of query aligns to 1:319/319 of 4i4iA

query
sites
4i4iA

M

M

K

K

K

R

I

I

A

G

V

V

L

L

T

T

S

S

G

G

G
|
G

D

D

A

S

P

P

G

G

M

M

N

N

A

A

C

A

I

I

R
|
R

A

S

V

V

R
|
R

T

K

G

A

I

I

F

Y

H

H

G

G

M

V

E

E

M

V

Y

Y

G

G

I

V

M

Y

Y

H

G

G

Y

Y

D

A

G

G

M

L

I

I

N

A

G

G

E

N

I

I

Q

K

K

K

M

L

E

E

S

V

H

G

A

D

V

V

S
x
G

N

D

I

I

V

I

Q

H

R

R

G

G

T

T

I

I

L

L

K

Y

S

T

A

A

R
|
R

S
x
C

E

P

K

E

F

F

R

K

T

T

K

E

E

E

G

G

R

Q

K

K

I

K

A

G

Y

I

E

E

Q

Q

L

L

Q

K

K

K

H

H

G

G

I

I

E

E

G

G

L

L

V

V

A

V

I

I

G

G

D
|
D

G
|
G

T

S

F

Y

T

Q

G

G

A

A

K

K

V

K

F

L

Y

-

E

T

E

E

Y

H

G

G

I

F

P

P

T

C

I

V

G

G

C

V

P

P

G
|
G

T
|
T

I

I

D
|
D

N

N

D
|
D

I

I

Y

P

G

G

T

T

D

D

F

F

T

T

I

I

G

G

F

F

D

D

T

T

A

A

I

L

N

N

T

T

A

V

L

I

E

D

A

A

I

I

D

D

K

K

I

I

R
|
R

D

D

T

A

A

A

A

T

A

S

H

H

D

E

R

R

I

T

F

Y

F

V

I

I

E

E

V

V

M

M

G

G

R
|
R

D

H

S

A

G

G

Y

D

I

I

A

A

V

L

E

W

C

S

G

G

L

L

G

A

G

G

A

A

E

E

M

T

V

I

M

L

V

I

P

P

E

E

T

A

K

D

T

Y

T

D

L

M

H

N

E

D

V

V

V

I

E

A

K

R

L

L

K

K

-

R

G

G

S

H

R

E

K
x
R

T

G

K
|
K

T

K

S
x
H

S

S

V

I

I

I

V

I

V

V

A

A

E

E

G

G

D

-

E

-

E

V

G

G

S

S

A

G

A

V

E

D

I

F

M

G

E

R

K

Q

V

I

K

Q

N

E

I

A

I

T

N

G

D

F

D

E

T

T

K

-

D

-

F

-

K

R

V

V

T

T

T

V

L

L

G

G

H

H

I

V

Q

Q

R

R

G

G

N

S

P

P

T

T

G

A

K

F

D

D

R

R

M

V

L

L

A

A

S

S

R

R

C

L

G

G

M

A

A

R

A

A

V

V

E

E

G

L

L

L

M

L

N

E

G

G

Q

K

A

G

N

G

C

R

M

C

A

V

G

G

I

I

I

Q

H

N

D

N

E

Q

V

L

V

V

Y

D

T

H

S

D

F

I

E

A

D

E

C

A

I

L

T

A

K

N

S

K

K

H

P

T

L

I

N

D

K

Q

D

R

T

M

L

Y

K

A

L

L

I

S

E

K

I

E

L

L

S

S

I

I

active site: G11 (= G11), R72 (= R72), C73 (≠ S73), D103 (= D103), G104 (= G104), G124 (= G125), T125 (= T126), D127 (= D128), D129 (= D130), R171 (= R172)
binding phosphoenolpyruvate: R21 (= R21), R25 (= R25), G58 (≠ S58), R154 (= R155), R211 (≠ K211), K213 (= K213), H215 (≠ S215)

5xz7A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
53% identity, 95% coverage: 1:307/324 of query aligns to 1:304/322 of 5xz7A

query
sites
5xz7A

M

M

K

K

I

I

A

A

V

V

L

L

T

T

S

S

G

G

D

D

A

S

P

P

G

G

M

M

N

N

A

A

C

A

I

V

R

R

A

A

V

V

R

R

T

T

G

A

I

I

F

Y

H

N

G

E

M

I

E

E

M

V

Y

Y

G

G

I

V

M

Y

Y

H

G

G

Y

Y

D

Q

G

G

M

L

I

L

N

N

G

D

E

D

I

I

Q

H

K

K

M

L

E

E

S

L

H

G

A

S

V

V

S

G

N

D

I

T

V

I

Q

Q

R

R

G

G

T

T

I

F

L

L

K

Y

S

S

A

A

R
|
R

S

C

E

P

K

E

F

F

R

K

T

E

K

Q

E

E

G

V

R

R

K

K

I

V

A

A

Y

I

E

E

Q

N

L

L

Q

R

K

K

H

R

G

G

I

I

E

E

G

G

L

L

V

V

A

V

I

I

G

G

D

D

G

G

T

S

F

Y

T

R

G

G

A

A

K

Q

V

R

F

I

Y

S

E

E

Y

C

G

K

-

E

I

I

P

Q

T

T

I

I

G

G

C

I

P

P

G

G

T

T

I

I

D
|
D

N

N

D

D

I

I

Y

N

G

G

T

T

D

D

F

F

T

T

I

I

G

G

F

F

D

D

T

T

A

A

I

L

N

N

T

T

A

I

L

I

E

G

A

L

I

V

D

D

K

K

I

I

R

R

D

D

T

T

A

A

A

S

H

H

D

A

R

R

I

T

F

F

F

I

I

I

E

E

V

A

M
|
M

G

G

R
|
R

D

D

S

C

G

G

Y

D

I

L

A

A

V

L

E

W

C

A

G

G

L

L

G

S

G

V

G

G

A

A

E

E

M

T

V

I

M

V

V

V

P

P

E

E

T

V

K

K

T

T

T

D

L

I

H

K

E

E

V

I

V

A

E

D

K

K

L

I

-

E

K

Q

G

G

S

I

R

K

K

R

T

G

K

K

T

K

S

H

S

S

V

I

I

V

V

L

V

V

A

A

E
|
E

G

G

D

C

E

M

E

-

G

-

S

T

A

A

A

Q

E

D

I

C

M

Q

E

K

K

E

V

L

K

S

N

Q

I

Y

I

I

N

N

D

V

D

D

T

N

K

-

D

-

F

-

K

R

V

V

T

S

T

V

L

L

G

G

H
|
H

I

V

Q

Q

R
|
R

G

G

N

S

P

P

T

T

G

G

K

A

D

D

R

R

M

V

L

L

A

A

S

S

R

R

C

L

G

G

M

G

A

Y

A

A

V

V

E

D

G

L

L

L

M

M

N

Q

G

G

Q

E

A

T

N

A

C

K

M

G

A

V

G

G

I

I

I

K

H

N

D

N

E

K

V

I

V

V

Y

A

T

T

S

S

F

F

E

D

D

E

C

I

I

F

T

D

K

K

binding 6-O-phosphono-beta-D-fructofuranose: R72 (= R72), D129 (= D128), M171 (= M170), R173 (= R172), E224 (= E222), H251 (= H254), R254 (= R257)

5xz6A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
53% identity, 95% coverage: 1:307/324 of query aligns to 1:304/318 of 5xz6A

query
sites
5xz6A

M

M

K

K

I

I

A

A

V

V

L

L

T

T

S

S

G

G

D

D

A

S

P

P

G

G

M

M

N

N

A

A

C

A

I

V

R

R

A

A

V

V

R

R

T

T

G

A

I

I

F

Y

H

N

G

E

M

I

E

E

M

V

Y

Y

G

G

I

V

M

Y

Y

H

G

G

Y
|
Y

D

Q

G

G

M

L

I

L

N

N

G

D

E

D

I

I

Q

H

K

K

M

L

E

E

S

L

H

G

A

S

V

V

S

G

N

D

I

T

V

I

Q

Q

R

R

G

G

T

T

I

F

L

L

K

Y

S

S

A

A

R
|
R

S
x
C

E

P

K

E

F

F

R
x
K

T

E

K

Q

E

E

G

V

R

R

K

K

I

V

A

A

Y

I

E

E

Q

N

L

L

Q

R

K

K

H

R

G

G

I

I

E

E

G

G

L

L

V

V

A

V

I

I

G

G

G
|
G

D

D

G
|
G

T
x
S

F

Y

T
x
R

G

G

A

A

K

Q

V

R

F

I

Y

S

E

E

Y

C

G

K

-

E

I

I

P

Q

T

T

I

I

G

G

C

I

P

P

G

G

T

T

I

I

D

D

N

N

D

D

I

I

Y

N

G

G

T

T

D

D

F

F

T

T

I

I

G

G

F

F

D

D

T

T

A

A

I

L

N

N

T

T

A

I

L

I

E

G

A

L

I

V

D

D

K

K

I

I

R

R

D

D

T

T

A

A

A

S

H

H

D

A

R

R

I

T

F

F

F

I

I

I

E

E

V

A

M

M

G

G

R
|
R

D

D

S

C

G

G

Y

D

I

L

A

A

V

L

E

W

C

A

G

G

L

L

G

S

G

V

G

G

A

A

E

E

M

T

V

I

M

V

V

V

P

P

E

E

T

V

K

K

T

T

T

D

L

I

H

K

E

E

V

I

V

A

E

D

K

K

L

I

-

E

K

Q

G

G

S

I

R

K

K

R

T

G

K

K

T

K

S

H

S

S

V

I

I

V

V

L

V

V

A

A

E

E

G

G

D

C

E

M

E

-

G

-

S

T

A

A

A

Q

E

D

I

C

M

Q

E

K

K

E

V

L

K

S

N

Q

I

Y

I

I

N

N

D

V

D

D

T

N

K

-

D

-

F

-

K

R

V

V

T

S

T

V

L

L

G

G

H

H

I

V

Q

Q

R

R

G

G

N

S

P

P

T

T

G

G

K

A

D

D

R

R

M

V

L

L

A

A

S

S

R

R

C

L

G

G

M

G

A

Y

A

A

V

V

E

D

G

L

L

L

M

M

N

Q

G

G

Q

E

A

T

N

A

C

K

M

G

A

V

G

G

I

I

I

K

H

N

D

N

E

K

V

I

V

V

Y

A

T

T

S

S

F

F

E

D

D

E

C

I

I

F

T

D

K

K

binding phosphoaminophosphonic acid-adenylate ester: Y41 (= Y41), R72 (= R72), C73 (≠ S73), K77 (≠ R77), G102 (= G102), G104 (= G104), S105 (≠ T105), R107 (≠ T107), R173 (= R172)

5xzaA Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adp (see paper)
51% identity, 100% coverage: 1:324/324 of query aligns to 1:322/322 of 5xzaA

query
sites
5xzaA

M

M

K

K

I

I

A

A

V

V

L

L

T

T

S
|
S

G

G

D

D

A

S

P

P

G

G

M

M

N

N

A

A

C

A

I

V

R

R

A

A

V

V

R

R

T

T

G

A

I

I

F

Y

H

N

G

E

M

I

E

E

M

V

Y

Y

G

G

I

V

M

Y

Y

H

G

G

Y
|
Y

D

Q

G

G

M

L

I

L

N

N

G

D

E

D

I

I

Q

H

K

K

M

L

E

E

S

L

H

G

A

S

V

V

S

G

N

D

I

T

V

I

Q

Q

R

R

G

G

T

T

I

F

L

L

K

Y

S

S

A

A

R
|
R

S
x
C

E

P

K

E

F
|
F

R

K

T

E

K

Q

E

E

G

V

R

R

K

K

I

V

A

A

Y

I

E

E

Q

N

L

L

Q

R

K

K

H

R

G

G

I

I

E

E

G

G

L

L

V

V

A

V

I

I

G

G

G
|
G

D

D

G
|
G

T
x
S

F

Y

T
x
R

G
|
G

A

A

K

Q

V

R

F

I

Y

S

E

E

Y

C

G

K

-

E

I

I

P

Q

T

T

I

I

G

G

C

I

P

P

G

G

T

T

I

I

D

D

N

N

D

D

I

I

Y

N

G

G

T

T

D

D

F

F

T

T

I

I

G

G

F

F

D

D

T

T

A

A

I

L

N

N

T

T

A

I

L

I

E

G

A

L

I

V

D

D

K

K

I

I

R

R

D

D

T

T

A

A

A

S

H

H

D

A

R

R

I

T

F

F

F

I

I

I

E

E

V

A

M

M

G

G

R

R

D

D

S

C

G

G

Y

D

I

L

A

A

V

L

E

W

C

A

G

G

L

L

G

S

G

V

G

G

A

A

E

E

M

T

V

I

M

V

V

V

P

P

E

E

T

V

K

K

T

T

T

D

L

I

H

K

E

E

V

I

V

A

E

D

K

K

L

I

-

E

K

Q

G

G

S

I

R

K

K

R

T

G

K

K

T

K

S

H

S

S

V

I

I

V

V

L

V

V

A

A

E

E

G

G

D

C

E

M

E

-

G

-

S

T

A

A

A

Q

E

D

I

C

M

Q

E

K

K

E

V

L

K

S

N

Q

I

Y

I

I

N

N

D

V

D

D

T

N

K

-

D

-

F

-

K

R

V

V

T

S

T

V

L

L

G

G

H

H

I

V

Q

Q

R

R

G

G

N

S

P

P

T

T

G

G

K

A

D

D

R

R

M

V

L

L

A

A

S

S

R

R

C

L

G

G

M

G

A

Y

A

A

V

V

E

D

G

L

L

L

M

M

N

Q

G

G

Q

E

A

T

N

A

C

K

M

G

A

V

G

G

I

I

I

K

H

N

D

N

E

K

V

I

V

V

Y

A

T

T

S

S

F

F

E

D

D

E

C

I

I

F

T

D

-

G

K

K

S

D

K

H

P

K

L

F

N

D

K

Y

D

S

T

L

L

Y

K

E

L

L

I

A

E

N

I

K

L

L

S

S

I

I

binding adenosine-5'-diphosphate: S9 (= S9), Y41 (= Y41), R72 (= R72), C73 (≠ S73), F76 (= F76), G102 (= G102), G104 (= G104), S105 (≠ T105), R107 (≠ T107), G108 (= G108)

Q2FXM8 ATP-dependent 6-phosphofructokinase; ATP-PFK; Phosphofructokinase; Phosphohexokinase; EC 2.7.1.11 from Staphylococcus aureus (strain NCTC 8325 / PS 47) (see paper)
51% identity, 100% coverage: 1:324/324 of query aligns to 1:322/322 of Q2FXM8

query
sites
Q2FXM8

M

M

K

K

I

I

A

A

V

V

L

L

T

T

S

S

G

G

D

D

A

S

P

P

G

G

M

M

N

N

A

A

C

A

I

V

R

R

A

A

V

V

R

R

T

T

G

A

I

I

F

Y

H

N

G

E

M

I

E

E

M

V

Y

Y

G

G

I

V

M

Y

Y

H

G

G

Y

Y

D

Q

G

G

M

L

I

L

N

N

G

D

E

D

I

I

Q

H

K

K

M

L

E

E

S

L

H

G

A

S

V

V

S

G

N

D

I

T

V

I

Q

Q

R

R

G

G

T

T

I

F

L

L

K

Y

S

S

A

A

R
|
R

S
x
C

E

P

K

E

F

F

R

K

T

E

K

Q

E

E

G

V

R

R

K

K

I

V

A

A

Y

I

E

E

Q

N

L

L

Q

R

K

K

H

R

G

G

I

I

E

E

G

G

L

L

V

V

A

V

I

I

G

G

G
|
G

D
|
D

G
|
G

T
x
S

F

Y

T

R

G

G

A

A

K

Q

V

R

F

I

Y

S

E

E

Y

C

G

K

-

E

I

I

P

Q

T

T

I

I

G

G

C

I

P

P

G

G

T
|
T

I
|
I

D
|
D

N

N

D

D

I

I

Y

N

G

G

T

T

D

D

F

F

T

T

I

I

G

G

F

F

D

D

T

T

A

A

I

L

N

N

T

T

A

I

L

I

E
x
G

A
x
L

I

V

D

D

K

K

I

I

R

R

D

D

T

T

A

A

A

S

H

H

D

A

R
|
R

I

T

F

F

F

I

I

I

E

E

V

A

M
|
M

G
|
G

R
|
R

D

D

S

C

G

G

Y

D

I

L

A

A

V

L

E

W

C

A

G

G

L

L

G

S

G

V

G

G

A

A

E

E

M

T

V

I

M

V

V

V

P

P

E

E

T

V

K

K

T

T

T

D

L

I

H

K

E

E

V

I

V

A

E

D

K

K

L

I

-

E

K

Q

G

G

S

I

R

K

K

R

T

G

K

K

T

K

S

H

S

S

V

I

I

V

V

L

V

V

A

A

E
|
E

G

G

D

C

E

M

E

-

G

-

S

T

A

A

A

Q

E

D

I

C

M

Q

E

K

K

E

V

L

K

S

N

Q

I

Y

I

I

N

N

D

V

D

D

T

N

K

-

D

-

F

-

K
x
R

V

V

T

S

T

V

L

L

G

G

H
|
H

I
x
V

Q
|
Q

R
|
R

G

G

N

S

P

P

T

T

G

G

K

A

D

D

R

R

M

V

L

L

A

A

S

S

R

R

C

L

G

G

M

G

A

Y

A

A

V

V

E

D

G

L

L

L

M

M

N

Q

G

G

Q

E

A

T

N

A

C

K

M

G

A

V

G

G

I

I

I

K

H

N

D

N

E

K

V

I

V

V

Y

A

T

T

S

S

F

F

E

D

D

E

C

I

I

F

T

D

-

G

K

K

S

D

K

H

P

K

L

F

N

D

K

Y

D

S

T

L

L

Y

K

E

L

L

I

A

E

N

I

K

L

L

S

S

I

I

RC 72:73 (≠ RS 72:73) binding
GDGS 102:105 (≠ GDGT 102:105) binding
TID 127:129 (= TID 126:128) binding in other chain
G150 (≠ E149) mutation to D: Exhibits higher affinity for fructose 6-phosphate and higher catalytic activity with a loss of dimer conversion; in association with A-151.
L151 (≠ A150) mutation to A: Exhibits higher affinity for fructose 6-phosphate and higher catalytic activity with a loss of tetramer-dimer conversion; in association with D-150.
R164 (= R163) mutation to A: Complete loss of fructose 6-phosphate binding.
MGR 171:173 (= MGR 170:172) binding in other chain
E224 (= E222) binding in other chain
R245 (≠ K248) mutation to A: Complete loss of fructose 6-phosphate binding.
HVQR 251:254 (≠ HIQR 254:257) binding in other chain

5xz9A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
53% identity, 94% coverage: 1:303/324 of query aligns to 1:300/321 of 5xz9A

query
sites
5xz9A

M

M

K

K

I

I

A

A

V

V

L

L

T

T

S
|
S

G

G

D

D

A

S

P

P

G

G

M

M

N

N

A

A

C

A

I

V

R

R

A

A

V

V

R

R

T

T

G

A

I

I

F

Y

H

N

G

E

M

I

E

E

M

V

Y

Y

G

G

I

V

M

Y

Y

H

G

G

Y
|
Y

D

Q

G

G

M

L

I

L

N

N

G

D

E

D

I

I

Q

H

K

K

M

L

E

E

S

L

H

G

A

S

V

V

S

G

N

D

I

T

V

I

Q

Q

R

R

G

G

T

T

I

F

L

L

K

Y

S

S

A

A

R

R

S
x
C

E

P

K

E

F
|
F

R
x
K

T

E

K

Q

E

E

G

V

R

R

K

K

I

V

A

A

Y

I

E

E

Q

N

L

L

Q

R

K

K

H

R

G

G

I

I

E

E

G

G

L

L

V

V

A

V

I

I

G

G

G
|
G

D
|
D

G
|
G

T
x
S

F

Y

T
x
R

G
|
G

A

A

K

Q

V

R

F

I

Y

S

E

E

Y

C

G

K

-

E

I

I

P

Q

T

T

I

I

G

G

C

I

P

P

G

G

T

T

I

I

D

D

N

N

D

D

I

I

Y

N

G

G

T

T

D

D

F

F

T

T

I

I

G

G

F

F

D

D

T

T

A

A

I

L

N

N

T

T

A

I

L

I

E

G

A

L

I

V

D

D

K

K

I

I

R

R

D

D

T

T

A

A

A

S

H

H

D

A

R

R

I

T

F

F

F

I

I

I

E

E

V

A

M

M

G

G

R

R

D

D

S

C

G

G

Y

D

I

L

A

A

V

L

E

W

C

A

G

G

L

L

G

S

G

V

G

G

A

A

E

E

M

T

V

I

M

V

V

V

P

P

E

E

T

V

K

K

T

T

T

D

L

I

H

K

E

E

V

I

V

A

E

D

K

K

L

I

-

E

K

Q

G

G

S

I

R

K

K

R

T

G

K

K

T

K

S

H

S

S

V

I

I

V

V

L

V

V

A

A

E

E

G

G

D

C

E

M

E

-

G

-

S

T

A

A

A

Q

E

D

I

C

M

Q

E

K

K

E

V

L

K

S

N

Q

I

Y

I

I

N

N

D

V

D

D

T

N

K

-

D

-

F

-

K

R

V

V

T

S

T

V

L

L

G

G

H

H

I

V

Q

Q

R

R

G

G

N

S

P

P

T

T

G

G

K

A

D

D

R

R

M

V

L

L

A

A

S

S

R

R

C

L

G

G

M

G

A

Y

A

A

V

V

E

D

G

L

L

L

M

M

N

Q

G

G

Q

E

A

T

N

A

C

K

M

G

A

V

G

G

I

I

I

K

H

N

D

N

E

K

V

I

V

V

Y

A

T

T

S

S

F

F

E

D

D

E

binding adenosine-5'-triphosphate: S9 (= S9), Y41 (= Y41), C73 (≠ S73), F76 (= F76), K77 (≠ R77), G102 (= G102), D103 (= D103), G104 (= G104), S105 (≠ T105), R107 (≠ T107), G108 (= G108)

1pfkA Crystal structure of the complex of phosphofructokinase from escherichia coli with its reaction products (see paper)
44% identity, 98% coverage: 1:316/324 of query aligns to 2:313/320 of 1pfkA

query
sites
1pfkA

M

I

K

K

I

I

A

G

V

V

L

L

T

T

S

S

G
|
G

D

D

A

A

P

P

G

G

M

M

N

N

A

A

C

A

I

I

R
|
R

A

G

V

V

R
|
R

T

S

G

A

I

L

F

T

H

E

G

G

M

L

E

E

M

V

Y

M

G

G

I

I

M

Y

Y

D

G

G

Y

Y

D

L

G

G

M

L

I

Y

N

E

G

D

E

R

I

M

Q

V

K

Q

M

L

E

D

S

R

H
x
Y

A

S

V

V

S
|
S

N
x
D

I

M

V

I

Q

N

R

R

G

G

T

T

I

F

L

L

K

G

S

S

A

A

R
|
R

S
x
F

E

P

K

E

F
|
F

R
|
R

T

D

K

E

E

N

G

I

R

R

K

A

I

V

A

A

Y

I

E

E

Q

N

L

L

Q

K

K

K

H

R

G

G

I

I

E

D

G

A

L

L

V

V

A

V

I

I

G

G

G
|
G

D
|
D

G
|
G

T
x
S

F

Y

T
x
M

G
|
G

A

A

K

-

V

M

F

R

Y

L

E

T

E

E

Y

M

G

G

I

F

P

P

T

C

I

I

G

G

C

L

P

P

G
|
G

T
|
T

I

I

D
|
D

N

N

D
|
D

I

I

Y

K

G

G

T

T

D

D

F

Y

T

T

I

I

G

G

F

F

D

F

T

T

A

A

I

L

N

S

T

T

A

V

L

V

E

E

A

A

I

I

D

D

K

R

I

L

R
|
R

D

D

T

T

A

S

H

H

D

Q

R

R

I

I

F

S

F

V

I

V

E

E

V

V

M
|
M

G

G

R
|
R

D

Y

S

C

G

G

Y

D

I

L

A

T

V

L

E

A

C

A

G

A

L

I

G

A

G

G

G
|
G

A

C

E
|
E

M

F

V

V

M

V

V

V

P

P

E

E

T

V

K

E

T

F

T

S

L

R

H

E

E

D

V

L

V

V

E

N

K

E

L

I

K

K

-

A

G

G

S

I

R

A

K

K

T
x
G

K
|
K

T

K

S
x
H

S

A

V

I

I

V

V

A

V

I

A

T

E
|
E

G

-

D

-

E

-

G

-

S

H

A

M

A

C

E

D

I

V

M

D

E

E

K

L

V

A

K

H

N

F

I

I

I

E

N

K

D

E

D

T

T

G

K

R

D

E

F

T

K

R

V

A

T

T

T

V

L

L

G

G

H
|
H

I

I

Q

Q

R
|
R

G

G

N

S

P

P

T

V

G

P

K

Y

D

D

R

R

M

I

L

L

A

A

S

S

R

R

C

M

G

G

M

A

A

Y

A

A

V

I

E

D

G

L

L

L

M

L

N

A

G

G

Q

Y

A

G

N

G

C

R

M

C

A

V

G

G

I

I

I

Q

H

N

D

E

E

Q

V

L

V

V

Y

H

T

H

S

D

F

I

E

I

D

D

C

A

I

I

T

E

K

N

S

M

K

K

-

R

P

P

L

F

N

K

K

G

D

D

T

W

L

L

active site: G12 (= G11), R73 (= R72), F74 (≠ S73), D104 (= D103), G105 (= G104), G125 (= G125), T126 (= T126), D128 (= D128), D130 (= D130), R172 (= R172)
binding adenosine-5'-diphosphate: G11 (= G10), R22 (= R21), R26 (= R25), Y56 (≠ H55), S59 (= S58), D60 (≠ N59), R73 (= R72), F74 (≠ S73), F77 (= F76), R78 (= R77), G103 (= G102), D104 (= D103), G105 (= G104), S106 (≠ T105), M108 (≠ T107), G109 (= G108), R155 (= R155), G213 (≠ T212), K214 (= K213), H216 (≠ S215)
binding 1,6-di-O-phosphono-beta-D-fructofuranose: G12 (= G11), R73 (= R72), T126 (= T126), D128 (= D128), M170 (= M170), E223 (= E222), H250 (= H254), R253 (= R257)
binding magnesium ion: G186 (= G186), E188 (= E188)

P0A796 ATP-dependent 6-phosphofructokinase isozyme 1; ATP-PFK 1; Phosphofructokinase 1; 6-phosphofructokinase isozyme I; Phosphohexokinase 1; Sedoheptulose-7-phosphate kinase; EC 2.7.1.11 from Escherichia coli (strain K12) (see 2 papers)
44% identity, 98% coverage: 1:316/324 of query aligns to 2:313/320 of P0A796

query
sites
P0A796

M

I

K

K

I

I

A

G

V

V

L

L

T

T

S

S

G

G

G
|
G

D

D

A

A

P

P

G

G

M

M

N

N

A

A

C

A

I

I

R
|
R

A
x
G

V
|
V

R
|
R

T

S

G

A

I

L

F

T

H

E

G

G

M

L

E

E

M

V

Y

M

G

G

I

I

M

Y

Y

D

G

G

Y

Y

D

L

G

G

M

L

I

Y

N

E

G

D

E

R

I

M

Q

V

K

Q

M

L

E

D

S
x
R

H
x
Y

A
x
S

V
|
V

S
|
S

N
x
D

I

M

V

I

Q

N

R

R

G

G

T

T

I

F

L

L

K

G

S

S

A

A

R
|
R

S
x
F

E

P

K

E

F

F

R

R

T

D

K

E

E

N

G

I

R

R

K

A

I

V

A

A

Y

I

E

E

Q

N

L

L

Q

K

K

K

H

R

G

G

I

I

E

D

G

A

L

L

V

V

A

V

I

I

G

G

G
|
G

D
|
D

G
|
G

T
x
S

F

Y

T

M

G

G

A

A

K

-

V

M

F

R

Y

L

E

T

E

E

Y

M

G

G

I

F

P

P

T

C

I

I

G

G

C

L

P

P

G

G

T
|
T

I
|
I

D
|
D

N

N

D

D

I

I

Y

K

G

G

T

T

D

D

F

Y

T

T

I

I

G

G

F

F

D

F

T

T

A

A

I

L

N

S

T

T

A

V

L

V

E

E

A

A

I

I

D

D

K

R

I

L

R
|
R

D

D

T

T

A

S

H

H

D

Q

R
|
R

I

I

F

S

F

V

I

V

E

E

V

V

M

M

G

G

R
|
R

D

Y

S

C

G

G

Y

D

I

L

A

T

V

L

E

A

C

A

G

A

L

I

G

A

G

G

G
|
G

A
x
C

E
|
E

M

F

V

V

M

V

V

V

P

P

E

E

T

V

K

E

T

F

T

S

L

R

H

E

E

D

V

L

V

V

E

N

K

E

L

I

K

K

-

A

G

G

S

I

R

A

K

K

T

G

K
|
K

T
x
K

S
x
H

S

A

V

I

I

V

V

A

V

I

A

T

E
|
E

G

-

D

-

E

-

G

-

S

H

A

M

A

C

E

D

I

V

M

D

E

E

K

L

V

A

K

H

N

F

I

I

I

E

N

K

D

E

D

T

T

G

K

R

D

E

F

T

K
x
R

V

A

T

T

T

V

L

L

G

G

H
|
H

I
|
I

Q
|
Q

R
|
R

G

G

N

S

P

P

T

V

G

P

K

Y

D

D

R

R

M

I

L

L

A

A

S

S

R

R

C

M

G

G

M

A

A

Y

A

A

V

I

E

D

G

L

L

L

M

L

N

A

G

G

Q

Y

A

G

N

G

C

R

M

C

A

V

G

G

I

I

I

Q

H

N

D

E

E

Q

V

L

V

V

Y

H

T

H

S

D

F

I

E

I

D

D

C

A

I

I

T

E

K

N

S

M

K

K

-

R

P

P

L

F

N

K

K

G

D

D

T

W

L

L

G12 (= G11) binding
RGVVR 22:26 (≠ RAVVR 21:25) binding
RYSVSD 55:60 (≠ SHAVSN 54:59) binding
RF 73:74 (≠ RS 72:73) binding
GDGS 103:106 (≠ GDGT 102:105) binding
D104 (= D103) binding
TID 126:128 (= TID 126:128) binding in other chain
D128 (= D128) active site, Proton acceptor; mutation to S: 18000-fold reduction of catalytic rate.
R155 (= R155) binding in other chain
R163 (= R163) binding
R172 (= R172) mutation to S: 3.4-fold reduction in turnover numbers.
GCE 186:188 (≠ GAE 186:188) binding in other chain
KKH 214:216 (≠ KTS 213:215) binding in other chain
E223 (= E222) binding in other chain
R244 (≠ K248) binding
HIQR 250:253 (= HIQR 254:257) binding in other chain

Q01813 ATP-dependent 6-phosphofructokinase, platelet type; ATP-PFK; PFK-P; 6-phosphofructokinase type C; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphohexokinase; EC 2.7.1.11 from Homo sapiens (Human) (see paper)
39% identity, 96% coverage: 2:313/324 of query aligns to 25:369/784 of Q01813

query
sites
Q01813

K

K

K

A

I

I

A

G

V

V

L

L

T

T

S

S

G

G

D

D

A

A

P

Q

G

G

M

M

N

N

A

A

C

A

I

V

R

R

A

A

V

V

R

R

T

M

G

G

I

I

F

Y

H

V

G

G

M

A

E

K

M

V

Y

Y

G

F

I

I

M

Y

Y

E

G

G

Y

Y

D

Q

G

G

M

M

I

V

N

D

G

G

-

G

-

S

E

N

I

I

Q

A

K

E

M

A

E

D

S

W

H

E

A

S

V

V

S

S

N

S

I

I

V

L

Q

Q

R

V

G

G

T

T

I

I

L

I

K

G

S

S

A

A

R

R

S

C

E

Q

K

A

F

F

R

R

T

T

K

R

E

E

G

G

R

R

K

L

I

K

A

A

Y

A

E

C

Q

N

L

L

Q

L

K

Q

H

R

G

G

I

I

E

T

G

N

L

L

V

C

A

V

I

I

G

G

D

D

G

G

T

S

F

L

T

T

G

G

A

A

K

N

V

L

F

F

Y

R

E

K

E

E

Y

W

G

S

-

G

-

L

-

E

-

L

-

A

-

R

-

N

-

G

-

Q

-

I

-

D

-

K

-

E

-

A

-

V

-

Q

-

K

-

Y

-

A

-

Y

I

L

P

N

T

V

I

V

G

G

C

M

P

V

G

G

T

S

I

I

D

D

N

N

D

D

I

F

Y

C

G

G

T

T

D

D

F

M

T

T

I

I

G

G

F

T

D

D

T

S

A

A

I

L

N

H

T

R

A

I

L

I

E

E

A

V

I

V

D

D

K

A

I

I

R

M

D

T

T

T

A

A

A

Q

A

S

H

H

D

Q

R

R

I

T

F

F

F

V

I

L

E

E

V

V

M

M

G

G

R

R

D

H

S

C

G

G

Y

Y

I

L

A

A

V

L

E

V

C

S

G

A

L

L

G

A

G

C

G

G

A

A

E

D

M

W

V

V

M

F

V

L

P

P

E

E

T

S

K

P

T

P

-

E

-

G

T

W

L

E

H

E

E

Q

V

M

V

C

E

V

K

K

L

L

K

S

G

E

S

N

R

R

-

A

K

R

T

K

K

K

T

R

S

L

S

N

V

I

I

I

V

I

V

V

A

A

E

E

G

G

D

A

E

I

E

D

G

T

S

Q

A

N

A

K

E

P

I

I

M

T

-

S

E

E

K

K

V

I

K

K

N

E

I

L

I

V

N

V

D

T

D

Q

T

L

-

G

K

Y

D

D

F

T

K

R

V

V

T

T

T

I

L

L

G

G

H

H

I

V

Q

Q

R

R

G

G

N

T

P

P

T

S

G

A

K

F

D

D

R

R

M

I

L

L

A

A

S

S

R

R

C

M

G

G

M

V

A

E

A

A

V

V

E

I

G

A

L

L

M

L

N

E

G

A

Q

T

A

P

N

D

-

T

-

P

-

A

C

C

M

V

A

V

G

S

I

L

I

N

H

G

D

N

E

H

V

A

V

V

Y

R

T

L

S

P

F

L

E

M

D

E

C

C

I

V

T

Q

K

M

S

T

K

Q

P

D

L

V

N

Q

K

K

Sites not aligning to the query:

386 modified: Phosphoserine; S→A: Decreased interaction with ATG4B.

4xykA Crystal structure of human phosphofructokinase-1 in complex with adp, northeast structural genomics consortium target hr9275 (see paper)
39% identity, 96% coverage: 2:313/324 of query aligns to 9:353/737 of 4xykA

query
sites
4xykA

K

K

K

A

I

I

A

G

V

V

L

L

T

T

S

S

G

G

G
|
G

D

D

A

A

P

Q

G

G

M

M

N

N

A

A

C

A

I

V

R
|
R

A

A

V

V

R
|
R

T

M

G

G

I

I

F

Y

H

V

G

G

M

A

E

K

M

V

Y

Y

G

F

I

I

M

Y

Y

E

G

G

Y
|
Y

D

Q

G

G

M

M

I

V

N

D

G

G

-

G

-

S

E

N

I

I

Q

A

K

E

M

A

E

D

S

W

H

E

A

S

V

V

S
|
S

N

S

I

I

V

L

Q

Q

R

V

G

G

T

T

I

I

L

I

K

G

S

S

A

A

R
|
R

S
x
C

E

Q

K

A

F

F

R
|
R

T

T

K

R

E

E

G

G

R

R

K

L

I

K

A

A

Y

A

E

C

Q

N

L

L

Q

L

K

Q

H

R

G

G

I

I

E

T

G

N

L

L

V

C

A

V

I

I

G

G

G
|
G

D
|
D

G
|
G

T
x
S

F

L

T

T

G

G

A

A

K

N

V

L

F

F

Y

R

E

K

E

E

Y

W

G

S

-

G

-

L

-

E

-

L

-

A

-

R

-

N

-

G

-

Q

-

I

-

D

-

K

-

E

-

A

-

V

-

Q

-

K

-

Y

-

A

-

Y

I

L

P

N

T

V

I

V

G

G

C

M

P

V

G
|
G

T
x
S

I

I

D
|
D

N

N

D
|
D

I

F

Y

C

G

G

T

T

D

D

F

M

T

T

I

I

G

G

F

T

D

D

T

S

A

A

I

L

N

H

T

R

A

I

L

I

E

E

A

V

I

V

D

D

K

A

I

I

R

M

D

T

T

T

A

A

A

Q

A

S

H

H

D

Q

R

R

I

T

F

F

F

V

I

L

E

E

V

V

M

M

G

G

R
|
R

D

H

S

C

G

G

Y

Y

I

L

A

A

V

L

E

V

C

S

G

A

L

L

G

A

G

C

G

G

A

A

E

D

M

W

V

V

M

F

V

L

P

P

E

E

T

S

K

P

T

P

-

E

-

G

T

W

L

E

H

E

E

Q

V

M

V

C

E

V

K

K

L

L

K

S

G

E

S

N

R

R

-

A

K

R

T

K

K
|
K

T

R

S

L

S

N

V

I

I

I

V

I

V

V

A

A

E

E

G

G

D

A

E

I

E

D

G

T

S

Q

A

N

A

K

E

P

I

I

M

T

-

S

E

E

K

K

V

I

K

K

N

E

I

L

I

V

N

V

D

T

D

Q

T

L

-

G

K

Y

D

D

F

T

K

R

V

V

T

T

T

I

L

L

G

G

H

H

I

V

Q

Q

R

R

G

G

N

T

P

P

T

S

G

A

K

F

D

D

R

R

M

I

L

L

A

A

S

S

R

R

C

M

G

G

M

V

A

E

A

A

V

V

E

I

G

A

L

L

M

L

N

E

G

A

Q

T

A

P

N

D

-

T

-

P

-

A

C

C

M

V

A

V

G

S

I

L

I

N

H

G

D

N

E

H

V

A

V

V

Y

R

T

L

S

P

F

L

E

M

D

E

C

C

I

V

T

Q

K

M

S

T

K

Q

P

D

L

V

N

Q

K

K

active site: G18 (= G11), R81 (= R72), C82 (≠ S73), D112 (= D103), G156 (= G125), S157 (≠ T126), D159 (= D128), D161 (= D130), R203 (= R172)
binding adenosine-5'-diphosphate: Y48 (= Y41), R81 (= R72), C82 (≠ S73), R86 (= R77), G111 (= G102), D112 (= D103), G113 (= G104), S114 (≠ T105)
binding phosphate ion: R28 (= R21), R32 (= R25), S67 (= S58), K248 (= K213)

Sites not aligning to the query:

binding phosphate ion: 414, 418, 451, 452

4xyjA Crystal structure of human phosphofructokinase-1 in complex with atp and mg, northeast structural genomics consortium target hr9275 (see paper)
39% identity, 96% coverage: 2:313/324 of query aligns to 13:357/768 of 4xyjA

query
sites
4xyjA

K

K

K

A

I

I

A

G

V

V

L

L

T

T

S

S

G
|
G

D

D

A

A

P

Q

G

G

M

M

N

N

A

A

C

A

I

V

R
|
R

A

A

V

V

R
|
R

T

M

G

G

I

I

F

Y

H

V

G

G

M

A

E

K

M

V

Y

Y

G

F

I

I

M

Y

Y

E

G

G

Y
|
Y

D

Q

G

G

M

M

I

V

N

D

G

G

-

G

-

S

E

N

I

I

Q

A

K

E

M

A

E

D

S

W

H

E

A

S

V

V

S

S

N
x
S

I

I

V

L

Q

Q

R

V

G

G

T

T

I

I

L

I

K

G

S

S

A

A

R
|
R

S
x
C

E

Q

K

A

F
|
F

R
|
R

T

T

K

R

E

E

G

G

R

R

K

L

I

K

A

A

Y

A

E

C

Q

N

L

L

Q

L

K

Q

H

R

G

G

I

I

E

T

G

N

L

L

V

C

A

V

I

I

G

G

G
|
G

D
|
D

G
|
G

T
x
S

F

L

T

T

G
|
G

A

A

K

N

V

L

F

F

Y

R

E

K

E

E

Y

W

G

S

-

G

-

L

-

E

-

L

-

A

-

R

-

N

-

G

-

Q

-

I

-

D

-

K

-

E

-

A

-

V

-

Q

-

K

-

Y

-

A

-

Y

I

L

P

N

T

V

I

V

G

G

C

M

P

V

G
|
G

T
x
S

I

I

D
|
D

N

N

D
|
D

I

F

Y

C

G

G

T

T

D

D

F

M

T

T

I

I

G

G

F

T

D

D

T

S

A

A

I

L

N

H

T

R

A

I

L

I

E

E

A

V

I

V

D

D

K

A

I

I

R

M

D

T

T

T

A

A

A

Q

A

S

H

H

D

Q

R

R

I

T

F

F

F

V

I

L

E

E

V

V

M

M

G

G

R
|
R

D

H

S

C

G

G

Y

Y

I

L

A

A

V

L

E

V

C

S

G

A

L

L

G

A

G

C

G
|
G

A

A

E

D

M

W

V

V

M

F

V

L

P

P

E

E

T

S

K

P

T

P

-

E

-

G

T

W

L

E

H

E

E

Q

V

M

V

C

E

V

K

K

L

L

K

S

G

E

S

N

R

R

-

A

K

R

T

K

K
|
K

T

R

S

L

S

N

V

I

I

I

V

I

V

V

A

A

E

E

G

G

D

A

E

I

E

D

G

T

S

Q

A

N

A

K

E

P

I

I

M

T

-

S

E

E

K

K

V

I

K

K

N

E

I

L

I

V

N

V

D

T

D

Q

T

L

-

G

K

Y

D

D

F

T

K

R

V

V

T

T

T

I

L

L

G

G

H

H

I

V

Q

Q

R

R

G

G

N

T

P

P

T

S

G

A

K

F

D

D

R

R

M

I

L

L

A

A

S

S

R

R

C

M

G

G

M

V

A

E

A

A

V

V

E

I

G

A

L

L

M

L

N

E

G

A

Q

T

A

P

N

D

-

T

-

P

-

A

C

C

M

V

A

V

G

S

I

L

I

N

H

G

D

N

E

H

V

A

V

V

Y

R

T

L

S

P

F

L

E

M

D

E

C

C

I

V

T

Q

K

M

S

T

K

Q

P

D

L

V

N

Q

K

K

active site: G22 (= G11), R85 (= R72), C86 (≠ S73), D116 (= D103), G160 (= G125), S161 (≠ T126), D163 (= D128), D165 (= D130), R207 (= R172)
binding adenosine-5'-triphosphate: G21 (= G10), G22 (= G11), Y52 (= Y41), C86 (≠ S73), F89 (= F76), R90 (= R77), G115 (= G102), D116 (= D103), G117 (= G104), S118 (≠ T105), G121 (= G108), S161 (≠ T126), R207 (= R172)
binding magnesium ion: G21 (= G10), G22 (= G11), D116 (= D103), D165 (= D130)
binding phosphate ion: R32 (= R21), R36 (= R25), S72 (≠ N59), G221 (= G186), K252 (= K213)

Sites not aligning to the query:

binding phosphate ion: 418, 456

4xyjF Crystal structure of human phosphofructokinase-1 in complex with atp and mg, northeast structural genomics consortium target hr9275 (see paper)
39% identity, 96% coverage: 2:313/324 of query aligns to 11:355/761 of 4xyjF

query
sites
4xyjF

K

K

K

A

I

I

A

G

V

V

L

L

T

T

S

S

G
|
G

D

D

A

A

P

Q

G

G

M

M

N

N

A

A

C

A

I

V

R
|
R

A

A

V

V

R
|
R

T

M

G

G

I

I

F

Y

H

V

G

G

M

A

E

K

M

V

Y

Y

G

F

I

I

M

Y

Y

E

G

G

Y
|
Y

D

Q

G

G

M

M

I

V

N

D

G

G

-

G

-

S

E

N

I

I

Q

A

K

E

M

A

E

D

S

W

H

E

A

S

V

V

S
|
S

N
x
S

I

I

V

L

Q

Q

R

V

G

G

T

T

I

I

L

I

K

G

S

S

A

A

R
|
R

S
x
C

E

Q

K

A

F
|
F

R
|
R

T

T

K

R

E

E

G

G

R

R

K

L

I

K

A

A

Y

A

E

C

Q

N

L

L

Q

L

K

Q

H

R

G

G

I

I

E

T

G

N

L

L

V

C

A

V

I

I

G
|
G

D
|
D

G
|
G

T
x
S

F

L

T

T

G
|
G

A

A

K

N

V

L

F

F

Y

R

E

K

E

E

Y

W

G

S

-

G

-

L

-

E

-

L

-

A

-

R

-

N

-

G

-

Q

-

I

-

D

-

K

-

E

-

A

-

V

-

Q

-

K

-

Y

-

A

-

Y

I

L

P

N

T

V

I

V

G

G

C

M

P

V

G
|
G

T
x
S

I

I

D
|
D

N

N

D
|
D

I

F

Y

C

G

G

T

T

D

D

F

M

T

T

I

I

G

G

F

T

D

D

T

S

A

A

I

L

N

H

T

R

A

I

L

I

E

E

A

V

I

V

D

D

K

A

I

I

R

M

D

T

T

T

A

A

A

Q

A

S

H

H

D

Q

R

R

I

T

F

F

F

V

I

L

E

E

V

V

M

M

G

G

R
|
R

D

H

S

C

G

G

Y

Y

I

L

A

A

V

L

E

V

C

S

G

A

L

L

G

A

G

C

G
|
G

A

A

E

D

M

W

V

V

M

F

V

L

P

P

E

E

T

S

K

P

T

P

-

E

-

G

T

W

L

E

H

E

E

Q

V

M

V

C

E

V

K

K

L

L

K

S

G

E

S

N

R

R

-

A

K

R

T

K

K
|
K

T

R

S

L

S

N

V

I

I

I

V

I

V

V

A

A

E

E

G

G

D

A

E

I

E

D

G

T

S

Q

A

N

A

K

E

P

I

I

M

T

-

S

E

E

K

K

V

I

K

K

N

E

I

L

I

V

N

V

D

T

D

Q

T

L

-

G

K

Y

D

D

F

T

K

R

V

V

T

T

T

I

L

L

G

G

H

H

I

V

Q

Q

R

R

G

G

N

T

P

P

T

S

G

A

K

F

D

D

R

R

M

I

L

L

A

A

S

S

R

R

C

M

G

G

M

V

A

E

A

A

V

V

E

I

G

A

L

L

M

L

N

E

G

A

Q

T

A

P

N

D

-

T

-

P

-

A

C

C

M

V

A

V

G

S

I

L

I

N

H

G

D

N

E

H

V

A

V

V

Y

R

T

L

S

P

F

L

E

M

D

E

C

C

I

V

T

Q

K

M

S

T

K

Q

P

D

L

V

N

Q

K

K

active site: G20 (= G11), R83 (= R72), C84 (≠ S73), D114 (= D103), G158 (= G125), S159 (≠ T126), D161 (= D128), D163 (= D130), R205 (= R172)
binding adenosine-5'-triphosphate: G20 (= G11), Y50 (= Y41), R83 (= R72), C84 (≠ S73), F87 (= F76), R88 (= R77), G113 (= G102), D114 (= D103), G115 (= G104), S116 (≠ T105), G119 (= G108), S159 (≠ T126)
binding magnesium ion: G19 (= G10), G20 (= G11), G112 (= G101), D114 (= D103), G158 (= G125), D163 (= D130), R205 (= R172)
binding phosphate ion: R30 (= R21), R34 (= R25), S69 (= S58), S70 (≠ N59), G219 (= G186), K250 (= K213)

Sites not aligning to the query:

binding cobalt (ii) ion: 475, 510, 511
binding phosphate ion: 416, 420, 453, 454

4rh3A Amppcp-bound structure of human platelet phosphofructokinase in an r- state, crystal form ii (see paper)
39% identity, 96% coverage: 4:313/324 of query aligns to 3:345/731 of 4rh3A

query
sites
4rh3A

I

I

A

G

V

V

L

L

T

T

S

S

G
|
G

D

D

A

A

P

Q

G

G

M

M

N

N

A

A

C

A

I

V

R

R

A

A

V

V

R

R

T

M

G

G

I

I

F

Y

H

V

G

G

M

A

E

K

M

V

Y

Y

G

F

I

I

M

Y

Y

E

G

G

Y

Y

D

Q

G

G

M

M

I

V

N

D

G

G

-

G

-

S

E

N

I

I

Q

A

K

E

M

A

E

D

S

W

H

E

A

S

V

V

S

S

N

S

I

I

V

L

Q

Q

R

V

G

G

T

T

I

I

L

I

K

G

S

S

A

A

R
|
R

S
x
C

E

Q

K

A

F
|
F

R
|
R

T

T

K

R

E

E

G

G

R

R

K

L

I

K

A

A

Y

A

E

C

Q

N

L

L

Q

L

K

Q

H

R

G

G

I

I

E

T

G

N

L

L

V

C

A

V

I

I

G

G

G
|
G

D
|
D

G
|
G

T
x
S

F

L

T

T

G
|
G

A

A

K

N

V

L

F

F

Y

R

E

K

E

E

Y

W

G

S

-

G

-

L

-

E

-

L

-

A

-

R

-

N

-

G

-

Q

-

I

-

D

-

K

-

E

-

A

-

V

-

Q

-

K

-

Y

-

A

-

Y

I

L

P

N

T

V

I

V

G

G

C

M

P

V

G
|
G

T
x
S

I

I

D
|
D

N

N

D
|
D

I

F

Y

C

G

G

T

T

D

D

F

M

T

T

I

I

G

G

F

T

D

D

T

S

A

A

I

L

N

H

T

R

A

I

L

I

E

E

A

V

I

V

D

D

K

A

I

I

R

M

D

T

T

T

A

A

A

Q

A

S

H

H

D

Q

R

R

I

T

F

F

F

V

I

L

E

E

V

V

M

M

G

G

R
|
R

D

H

S

C

G

G

Y

Y

I

L

A

A

V

L

E

V

C

S

G

A

L

L

G

A

G

C

G

G

A

A

E

D

M

W

V

V

M

F

V

L

P

P

E

E

T

S

K

P

T

P

-

E

-

G

T

W

L

E

H

E

E

Q

V

M

V

C

E

V

K

K

L

L

K

S

G

E

S

N

R

R

-

A

K

R

T

K

K
|
K

T

R

S

L

S

N

V

I

I

I

V

I

V

V

A

A

E

E

G

G

D

A

E

I

E

D

G

T

S

Q

A

N

A

K

E

P

I

I

M

T

-

S

E

E

K

K

V

I

K

K

N

E

I

L

I

V

N

V

D

T

D

Q

T

L

-

G

K

Y

D

D

F

T

K

R

V

V

T

T

T

I

L

L

G

G

H

H

I

V

Q

Q

R

R

G

G

N

T

P

P

T

S

G

A

K

F

D

D

R

R

M

I

L

L

A

A

S

S

R

R

C

M

G

G

M

V

A

E

A

A

V

V

E

I

G

A

L

L

M

L

N

E

G

A

Q

T

A

P

N

D

-

T

-

P

-

A

C

C

M

V

A

V

G

S

I

L

I

N

H

G

D

N

E

H

V

A

V

V

Y

R

T

L

S

P

F

L

E

M

D

E

C

C

I

V

T

Q

K

M

S

T

K

Q

P

D

L

V

N

Q

K

K

active site: G10 (= G11), R73 (= R72), C74 (≠ S73), D104 (= D103), G148 (= G125), S149 (≠ T126), D151 (= D128), D153 (= D130), R195 (= R172)
binding phosphomethylphosphonic acid adenylate ester: G9 (= G10), R73 (= R72), C74 (≠ S73), F77 (= F76), R78 (= R77), G103 (= G102), D104 (= D103), G105 (= G104), S106 (≠ T105), G109 (= G108)
binding phosphate ion: K240 (= K213)

Sites not aligning to the query:

binding phosphate ion: 406, 410, 443, 444, 457, 552, 641, 647

P17858 ATP-dependent 6-phosphofructokinase, liver type; ATP-PFK; PFK-L; 6-phosphofructokinase type B; Phosphofructo-1-kinase isozyme B; PFK-B; Phosphohexokinase; EC 2.7.1.11 from Homo sapiens (Human) (see 3 papers)
39% identity, 96% coverage: 2:313/324 of query aligns to 16:360/780 of P17858

query
sites
P17858

K

K

K

A

I

I

A

G

V

V

L

L

T

T

S

S

G

G

D

D

A

A

P

Q

G

G

M

M

N

N

A

A

C

A

I

V

R

R

A

A

V

V

V

T

R

R

T

M

G

G

I

I

F

Y

H

V

G

G

M

A

E

K

M

V

Y

F

G

L

I

I

M

Y

Y

E

G

G

Y

Y

D

E

G

G

M

L

I

V

N

E

G

G

-

G

-

E

E

N

I

I

Q

K

K

Q

M

A

E

N

S

W

H

L

A

S

V

V

S

S

N

N

I

I

V

I

Q

Q

R

L

G

G

G
|
G

T

T

I

I

L

I

K

G

S

S

A

A

R

R

S

C

E

K

K

A

F

F

R

T

T

T

K

R

E

E

G

G

R

R

K

R

I

A

A

A

Y

A

E

Y

Q

N

L

L

Q

V

K

Q

H

H

G

G

I

I

E

T

G

N

L

L

V

C

A

V

I

I

G

G

D

D

G

G

T

S

F

L

T

T

G

G

A

A

K

N

V

I

F

F

Y

R

E

S

E

E

Y

W

G

G

-

S

-

L

-

E

-

L

-

V

-

A

-

E

-

G

-

K

-

I

-

S

-

E

-

T

-

A

-
x
R

-

T

-

Y

-

S

-

H

I

L

P

N

T

I

I

A

G

G

C

L

P

V

G

G

T

S

I

I

D

D

N

N

D

D

I

F

Y

C

G

G

T

T

D

D

F

M

T

T

I

I

G

G

F

T

D

D

T

S

A

A

I

L

N

H

T

R

A

I

L

M

E

E

A

V

I

I

D

D

K

A

I

I

R

T

D

T

T

T

A

A

A

Q

A

S

H

H

D

Q

R

R

I

T

F

F

F

V

I

L

E

E

V

V

M

M

G

G

R

R

D

H

S

C

G

G

Y

Y

I

L

A

A

V

L

E

V

C

S

G

A

L

L

G

A

G

S

G

G

A

A

E

D

M

W

V

L

M

F

V

I

P

P

E

E

T

A

K

P

T

P

-

E

-

D

-

G

T

W

L

E

H

N

E

F

V

M

V

C

E

E

K

R

L

L

K

G

G

E

S

T

R

R

K

S

T

R

K

G

T

S

S

R

-

L

S

N

V

I

I

I

V

I

A

A

E

E

G

G

-

A

-

I

D

D

E

R

E

N

G

G

-

K

-

P

-

I

S

S

A

S

E

Y

I

V

M

K

E

D

K

L

V

V

K

V

N

Q

I

R

I

L

N

G

D

F

D

D

T

T

K

-

D

-

F

-

K

R

V

V

T

T

T

V

L

L

G

G

H

H

I

V

Q

Q

R

R

G

G

N

T

P

P

T

S

G

A

K

F

D

D

R

R

M

I

L

L

A

S

S

S

R

K

C

M

G

G

M

M

A

E

A

A

V

V

E

M

G

A

L

L

M

L

N

E

G

A

Q

T

A

P

N

D

-

T

-

P

-

A

C

C

M

V

A

V

G

T

I

L

I

S

H

G

D

N

E

Q

V

S

V

V

Y

R

T

L

S

P

F

L

E

M

D

E

C

C

I

V

T

Q

K

M

S

T

K

K

P

E

L

V

N

Q

K

K

G81 (= G65) natural variant: G -> A
R151 (vs. gap) to W: in dbSNP:rs755851304

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
527 T→A: Does not affect GlcNAcylation.
529 modified: carbohydrate, O-linked (GlcNAc) serine; S→A: Prevents GlcNAcylation and enhance enzyme activity.

7lw1A Human phosphofructokinase-1 liver type bound to activator na-11 (see paper)
39% identity, 96% coverage: 2:313/324 of query aligns to 6:350/744 of 7lw1A

query
sites
7lw1A

K

K

K

A

I

I

A

G

V

V

L

L

T

T

S

S

G

G

D

D

A

A

P

Q

G

G

M

M

N

N

A

A

C

A

I

V

R

R

A

A

V

V

V

T

R

R

T

M

G

G

I

I

F

Y

H

V

G

G

M

A

E

K

M

V

Y

F

G

L

I

I

M

Y

Y

E

G

G

Y

Y

D

E

G

G

M

L

I

V

N

E

G

G

-

G

-

E

E

N

I

I

Q

K

K

Q

M

A

E

N

S

W

H

L

A

S

V

V

S

S

N

N

I

I

V

I

Q

Q

R

L

G

G

T

T

I

I

L

I

K

G

S

S

A

A

R
|
R

S
x
C

E

K

K

A

F

F

R
x
T

T

T

K

R

E

E

G

G

R

R

K

R

I

A

A

A

Y

A

E

Y

Q

N

L

L

Q

V

K

Q

H

H

G

G

I

I

E

T

G

N

L

L

V

C

A

V

I

I

G

G

G
|
G

D

D

G
|
G

T
x
S

F

L

T

T

G
|
G

A

A

K

N

V
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binding adenosine-5'-diphosphate: R78 (= R72), C79 (≠ S73), T83 (≠ R77), G108 (= G102), G110 (= G104), S111 (≠ T105), G114 (= G108), I117 (≠ V111)
binding 6-O-phosphono-beta-D-fructofuranose: I155 (= I127), D156 (= D128), R191 (= R163), M198 (= M170), R200 (= R172), E254 (= E222), R282 (≠ K248), H288 (= H254), R291 (= R257)
binding N-{(11S)-2-[2-(5-hydroxypent-1-yn-1-yl)phenyl]-4H,10H-pyrazolo[5,1-c][1,4]benzoxazepin-7-yl}acetamide: M164 (≠ F136), F298 (≠ K264), I301 (≠ M267), K305 (≠ R271), N331 (≠ D294)

Sites not aligning to the query:

Query Sequence

>Echvi_2325 FitnessBrowser__Cola:Echvi_2325
MKKIAVLTSGGDAPGMNACIRAVVRTGIFHGMEMYGIMYGYDGMINGEIQKMESHAVSNI
VQRGGTILKSARSEKFRTKEGRKIAYEQLQKHGIEGLVAIGGDGTFTGAKVFYEEYGIPT
IGCPGTIDNDIYGTDFTIGFDTAINTALEAIDKIRDTAAAHDRIFFIEVMGRDSGYIAVE
CGLGGGAEMVMVPETKTTLHEVVEKLKGSRKTKTSSVIVVAEGDEEGSAAEIMEKVKNII
NDDTKDFKVTTLGHIQRGGNPTGKDRMLASRCGMAAVEGLMNGQANCMAGIIHDEVVYTS
FEDCITKSKPLNKDTLKLIEILSI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory