SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_2361 FitnessBrowser__Cola:Echvi_2361 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ho9A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-galactose and utp
66% identity, 100% coverage: 1:284/285 of query aligns to 1:285/294 of 4ho9A

query
sites
4ho9A

M

M

K

K

G

G

I

I

L
|
L

A

S

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

L

T

T

I

K

A

V

V

V

S

S

K

K

Q

Q

L

L

M

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

M
x
L

A

A

G

G

I

I

R

K

E

D

I

I

M

L

I

I

T

S

T

T

P

P

E

E

D

D

A

T

D

P

A

R

F

F

K

E

K

Q

L

L

G

G

D

G

G

G

S

S

H

E

L

L

G

G

C

I

E

S

F

L

T

S

F

Y

A

A

I

V

Q
|
Q

P

S

K

S

P
|
P

E

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

V

I

I

I

G

G

E

E

F

F

I

I

G

G

D

D

K

N

V

V

A

A

L

L

I

V

L
|
L

G

G

D
|
D

N

N

I

I

F

F

Y

Y

G
|
G

S
x
H

G
|
G

L

F

Q
x
T

E

E

T

L

L

L

L

Q

K

R

H

A

T

A

D

N

P

R

E

K

-

S

G

G

G

A

V

T

V

I

F

F

A

G

Y

Y

H

N

V

V

N

K

D

D

P

P

Q

Q

R

R

Y

F

G
|
G

V

V

E

E

F

F

D

D

Q

E

N

K

K

G

K

K

A

V

I

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

Q

E

A

E

P

P

K

K

S

S

N

S

F

Y

A

A

V
|
V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

R

R

V

V

E

D

I

I

A

A

K

K

Q

N

I

I

K

T

P

P

S

S

A

A

R
|
R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

V

N

N

N

K

A

A

Y

Y

L

L

K

E

E

L

G

G

K

E

L

L

S

H

V

V

G

E

I

L

L

L

S
x
G

R
|
R

G
|
G

T

F

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

Q

E

S

S

L

L

Q

L

Q

Q

A

A

G

S

Q

Q

F

F

V

I

E

E

V

T

I

I

E

E

E

K

R

R

Q

Q

G

S

L

L

K

K

I

V

G

A

C

C

V

L

E

E

V
x
I

A

A

Y

Y

H

R

K

M

G

G

F

Y

I

I

T

S

K

R

Q

E

Q

Q

L

L

L

I

A

K

Q

L

A

A

E

E

K

P

L

L

G

M

K

K

S

N

G

E

Y

Y

G

G

T

Q

Y

Y

L

L

K

M

G

N

I

L

binding galactose-uridine-5'-diphosphate: L6 (= L6), G8 (= G8), G9 (= G9), Q80 (= Q80), P83 (= P83), G85 (= G85), L86 (= L86), L106 (= L106), D108 (= D108), G144 (= G143), E159 (= E158), K160 (= K159), V170 (= V169), R192 (= R191), W221 (= W220)
binding uridine 5'-triphosphate: L43 (≠ M43), G113 (= G113), H114 (≠ S114), G115 (= G115), T117 (≠ Q117), G216 (≠ S215), R217 (= R216), G218 (= G217), I254 (≠ V253)

4ho4A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with thymidine and glucose-1-phosphate
66% identity, 100% coverage: 1:284/285 of query aligns to 1:285/289 of 4ho4A

query
sites
4ho4A

M

M

K

K

G

G

I

I

L

L

A

S

G

G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

L

T

T

I

K

A

V

V

V

S

S

K

K

Q

Q

L

L

M

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

M

L

A

A

G

G

I

I

R

K

E

D

I

I

M

L

I

I

T

S

T

T

P

P

E

E

D

D

A

T

D

P

A

R

F

F

K

E

K

Q

L

L

G

G

D

G

G

G

S

S

H

E

L

L

G

G

C

I

E

S

F

L

T

S

F

Y

A

A

I

V

Q

Q

P

S

K

S

P

P

E

D

G

G

L

L

A

A

Q

Q

A

A

F

F

V

I

I

I

G

G

E

E

F

F

I

I

G

G

D

D

K

N

V

V

A

A

L

L

I

V

L
|
L

G

G

D

D

N

N

I

I

F

F

Y

Y

G

G

S

H

G

G

L

F

Q

T

E

E

T

L

L

L

L

Q

K

R

H

A

T

A

D

N

P

R

E

K

-

S

G

G

G

A

V

T

V

I

F

F

A

G

Y

Y

H

N

V

V

N

K

D

D

P

P

Q

Q

R

R

Y
x
F

G
|
G

V

V

E

E

F

F

D

D

Q

E

N

K

K

G

K

K

A

V

I

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

Q

E

A

E

P

P

K

K

S

S

N

S

F

Y

A

A

V
|
V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

R

R

V

V

E

D

I

I

A

A

K

K

Q

N

I

I

K

T

P

P

S

S

A

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

V

N

N

N

K

A

A

Y

Y

L

L

K

E

E

L

G

G

K

E

L

L

S

H

V

V

G

E

I

L

L

L

S

G

R

R

G

G

T

F

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

Q

E

S

S

L

L

Q

L

Q

Q

A

A

G

S

Q

Q

F

F

V

I

E

E

V

T

I

I

E

E

E

K

R

R

Q

Q

G

S

L

L

K

K

I

V

G

A

C

C

V

L

E

E

V

I

A

A

Y

Y

H

R

K

M

G

G

F

Y

I

I

T

S

K

R

Q

E

Q

Q

L

L

L

I

A

K

Q

L

A

A

E

E

K

P

L

L

G

M

K

K

S

N

G

E

Y

Y

G

G

T

Q

Y

Y

L

L

K

M

G

N

I

L

binding 1-O-phosphono-alpha-D-glucopyranose: L106 (= L106), F143 (≠ Y142), G144 (= G143), E159 (= E158), K160 (= K159), V170 (= V169), W221 (= W220)

4ho6A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-glucose and utp
66% identity, 100% coverage: 1:284/285 of query aligns to 1:285/288 of 4ho6A

query
sites
4ho6A

M

M

K

K

G

G

I

I

L
|
L

A

S

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

L

T

T

I

K

A

V

V

V

S

S

K

K

Q

Q

L

L

M

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

M
x
L

A

A

G

G

I

I

R

K

E

D

I

I

M

L

I

I

T

S

T

T

P

P

E

E

D

D

A

T

D

P

A

R

F

F

K

E

K

Q

L

L

G

G

D

G

G

G

S

S

H

E

L

L

G

G

C

I

E

S

F

L

T

S

F

Y

A

A

I

V

Q
|
Q

P

S

K

S

P
|
P

E

D

G
|
G

L

L

A

A

Q

Q

A

A

F

F

V

I

I

I

G

G

E

E

F

F

I

I

G

G

D

D

K

N

V

V

A

A

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

S
x
H

G
|
G

L

F

Q
x
T

E

E

T

L

L

L

L

Q

K

R

H

A

T

A

D

N

P

R

E

K

-

S

G

G

G

A

V

T

V

I

F

F

A

G

Y

Y

H

N

V

V

N

K

D

D

P

P

Q

Q

R

R

Y

F

G
|
G

V

V

E

E

F

F

D

D

Q

E

N

K

K

G

K

K

A

V

I

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

Q

E

A

E

P

P

K

K

S

S

N

S

F

Y

A

A

V
|
V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

R

R

V

V

E

D

I

I

A

A

K

K

Q

N

I

I

K

T

P

P

S

S

A

A

R
|
R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

V

N

N

N

K

A

A

Y

Y

L

L

K

E

E

L

G

G

K

E

L

L

S

H

V

V

G

E

I

L

L

L

S

G

R

R

G

G

T

F

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

Q

E

S

S

L

L

Q

L

Q

Q

A

A

G

S

Q

Q

F

F

V

I

E

E

V

T

I

I

E

E

E

K

R

R

Q

Q

G

S

L

L

K

K

I

V

G

A

C

C

V

L

E

E

E
|
E

V
x
I

A

A

Y

Y

H

R

K

M

G

G

F

Y

I

I

T

S

K

R

Q

E

Q

Q

L

L

L

I

A

K

Q

L

A

A

E

E

K

P

L

L

G

M

K

K

S

N

G

E

Y

Y

G

G

T

Q

Y

Y

L

L

K

M

G

N

I

L

binding uridine-5'-diphosphate-glucose: L6 (= L6), G8 (= G8), G9 (= G9), Q80 (= Q80), P83 (= P83), G85 (= G85), D108 (= D108), G144 (= G143), E159 (= E158), K160 (= K159), V170 (= V169), R192 (= R191)
binding uridine 5'-triphosphate: L43 (≠ M43), Y112 (= Y112), G113 (= G113), H114 (≠ S114), G115 (= G115), T117 (≠ Q117), E253 (= E252), I254 (≠ V253)

4ho5A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with tdp-glucose
66% identity, 100% coverage: 1:284/285 of query aligns to 1:285/288 of 4ho5A

query
sites
4ho5A

M

M

K

K

G

G

I

I

L
|
L

A

S

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

L

T

T

I

K

A

V

V

V

S

S

K

K

Q
|
Q

L

L

M

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

M

L

A

A

G

G

I

I

R

K

E

D

I

I

M

L

I

I

T

S

T

T

P

P

E

E

D

D

A

T

D

P

A

R

F

F

K

E

K

Q

L

L

G

G

D

G

G

G

S

S

H

E

L

L

G

G

C

I

E

S

F

L

T

S

F

Y

A

A

I

V

Q
|
Q

P

S

K

S

P
|
P

E

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

V

I

I

I

G

G

E

E

F

F

I

I

G

G

D

D

K

N

V

V

A

A

L

L

I

V

L
|
L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

S
x
H

G
|
G

L
x
F

Q
x
T

E

E

T

L

L

L

L

Q

K

R

H

A

T

A

D

N

P

R

E

K

-

S

G

G

G

A

V

T

V

I

F

F

A

G

Y

Y

H

N

V

V

N

K

D

D

P

P

Q

Q

R

R

Y
x
F

G
|
G

V

V

E

E

F

F

D

D

Q

E

N

K

K

G

K

K

A

V

I

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

Q

E

A

E

P

P

K

K

S

S

N

S

F

Y

A

A

V
|
V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

R

R

V

V

E

D

I

I

A

A

K

K

Q

N

I

I

K

T

P

P

S

S

A

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

V

N

N

N

K

A

A

Y

Y

L

L

K

E

E

L

G

G

K

E

L

L

S

H

V

V

G

E

I

L

L

L

S
x
G

R
|
R

G
|
G

T

F

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

Q

E

S

S

L

L

Q

L

Q

Q

A

A

G

S

Q

Q

F

F

V

I

E

E

V

T

I

I

E

E

E

K

R

R

Q

Q

G

S

L

L

K

K

I

V

G

A

C

C

V

L

E

E

E
|
E

V
x
I

A

A

Y

Y

H

R

K

M

G

G

F

Y

I

I

T

S

K

R

Q

E

Q

Q

L

L

L

I

A

K

Q

L

A

A

E

E

K

P

L

L

G

M

K

K

S

N

G

E

Y

Y

G

G

T

Q

Y

Y

L

L

K

M

G

N

I

L

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: L6 (= L6), G8 (= G8), G9 (= G9), Q24 (= Q24), Q80 (= Q80), P83 (= P83), G85 (= G85), L86 (= L86), L106 (= L106), D108 (= D108), Y112 (= Y112), G113 (= G113), H114 (≠ S114), G115 (= G115), F116 (≠ L116), T117 (≠ Q117), F143 (≠ Y142), G144 (= G143), E159 (= E158), K160 (= K159), V170 (= V169), G216 (≠ S215), R217 (= R216), G218 (= G217), W221 (= W220), E253 (= E252), I254 (≠ V253)

4ho3A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with thymidine triphosphate
66% identity, 100% coverage: 1:284/285 of query aligns to 1:285/288 of 4ho3A

query
sites
4ho3A

M

M

K

K

G

G

I

I

L
|
L

A

S

G
|
G

S

S

G
|
G

T
|
T

R
|
R

L

L

Y

Y

P

P

L

L

T

T

I

K

A

V

V

V

S

S

K

K

Q
|
Q

L

L

M

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

M
x
L

A

A

G

G

I

I

R

K

E

D

I

I

M

L

I

I

T

S

T

T

P

P

E

E

D

D

A

T

D

P

A

R

F

F

K

E

K

Q

L

L

G

G

D

G

G

G

S

S

H

E

L

L

G

G

C

I

E

S

F

L

T

S

F

Y

A

A

I

V

Q
|
Q

P

S

K

S

P
|
P

E

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

V

I

I

I

G

G

E

E

F

F

I

I

G

G

D

D

K

N

V

V

A

A

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

S
x
H

G
|
G

L
x
F

Q
x
T

E

E

T

L

L

L

L

Q

K

R

H

A

T

A

D

N

P

R

E

K

-

S

G

G

G

A

V

T

V

I

F

F

A

G

Y

Y

H

N

V

V

N

K

D

D

P

P

Q

Q

R

R

Y

F

G

G

V

V

E

E

F

F

D

D

Q

E

N

K

K

G

K

K

A

V

I

I

S

S

I

I

E

E

K

K

P

P

Q

E

A

E

P

P

K

K

S

S

N

S

F

Y

A

A

V

V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

R

R

V

V

E

D

I

I

A

A

K

K

Q

N

I

I

K

T

P

P

S

S

A

A

R

R

G

G

E
|
E

L

L

E

E

I

I

T

T

D

D

V

V

N

N

N

K

A

A

Y

Y

L

L

K

E

E

L

G

G

K

E

L

L

S

H

V

V

G

E

I

L

L

L

S

G

R

R

G

G

T

F

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

Q

E

S

S

L

L

Q

L

Q

Q

A

A

G

S

Q

Q

F

F

V

I

E

E

V

T

I

I

E

E

E

K

R

R

Q

Q

G

S

L

L

K

K

I

V

G

A

C

C

V

L

E

E

E
|
E

V
x
I

A

A

Y

Y

H
x
R

K

M

G

G

F

Y

I

I

T

S

K

R

Q

E

Q

Q

L

L

L

I

A

K

Q

L

A

A

E

E

K

P

L

L

G

M

K

K

S

N

G

E

Y

Y

G

G

T

Q

Y

Y

L

L

K

M

G

N

I

L

binding thymidine-5'-triphosphate: L6 (= L6), G8 (= G8), G9 (= G9), G11 (= G11), T12 (= T12), R13 (= R13), Q24 (= Q24), L43 (≠ M43), Q80 (= Q80), P83 (= P83), G85 (= G85), L86 (= L86), D108 (= D108), Y112 (= Y112), G113 (= G113), H114 (≠ S114), G115 (= G115), F116 (≠ L116), T117 (≠ Q117), E194 (= E193), E253 (= E252), I254 (≠ V253), R257 (≠ H256)

P26393 Glucose-1-phosphate thymidylyltransferase; dTDP-glucose pyrophosphorylase; Ep; dTDP-glucose synthase; EC 2.7.7.24 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
65% identity, 98% coverage: 2:281/285 of query aligns to 5:285/292 of P26393

query
sites
P26393

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

V

T

T

I

M

A

A

V

V

S

S

K

K

Q

Q

L

L

M

L

P

P

V

I

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

M

L

A

A

G

G

I

I

R

R

E

D

I

I

M

L

I

I

T

S

T

T

P

P

E

Q

D

D

A

T

D

P

A

R

F

F

K

Q

L

L

G

G

D

D

G

G

S

S

H

Q

L

W

G

G

C

L

E

N

F

L

T

Q

F

Y

A

K

I

V

Q

Q

P

P

K

S

P

P

E

D

G

G

L

L

A

A

Q

Q

A

A

F

F

V

I

I

I

G

G

E

E

F

F

I

I

G

G

D

H

D

D

K

D

V

C

A

A

L

L

I

V

L

L

G

G

D

D

N

N

I

I

F

F

Y

Y

G

G

S

H

G

D

L

L

Q

P

E

K

T

L

L

M

L

E

K

A

H

A

T

V

D

N

P

K

E

E

-

S

G

G

G

A

V

T

V

V

F

F

A

A

Y

Y

H

H

V

V

N

N

D

D

P

P

Q

E

R

R

Y

Y

G

G

V

V

E

E

F

F

D

D

Q

Q

N

K

K

G

K

T

A

A

I

V

S

S

I

L

E

E

K

K

P

P

Q

L

A

Q

P

P

K

K

S

S

N

N

F

Y

A

A

V

V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

R

S

V

V

E

E

I

M

A

A

K

K

Q

N

I

L

K

K

P

P

S

S

A

A

R

R

G

G

E

E

L

L

E

E

I

I

T
|
T

D

D

V

I

N

N

N

R

A

I

Y

Y

L

M

K

E

E

Q

G

G

K

R

L

L

S

S

V

V

G

A

I

M

L

M

S

G

R

R

G

G

T

Y

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

Q

Q

S

S

L

L

Q

I

Q

E

A

A

G

S

Q

N

F

F

V

I

E

A

V

T

I

I

E

E

R

R

Q

Q

G

G

L

L

K

K

I

V

G

S

C

C

V

P

E

E

V

I

A

A

Y

F

H

R

K

K

G

N

F

F

I

I

T

N

K

A

Q

Q

L

V

L

I

A

E

Q

L

A

A

E

G

K

P

L

L

G

S

K

K

S

N

G

D

Y

Y

G

G

T

K

Y

Y

L

L

T201 (= T197) mutation to A: Two-fold increase in the conversion of 2-acetamido-2-deoxy-alpha-D-glucopyranosyl phosphate.
W224 (= W220) mutation to H: Is able to convert both 6-acetamido-6-deoxy-alpha-D-glucopyranosyl phosphate and alpha-D-glucopyranuronic acid 1-(dihydrogen phosphate), which are not accepted by the wild-type.

1iinA Thymidylyltransferase complexed with udp-glucose (see paper)
65% identity, 98% coverage: 2:281/285 of query aligns to 5:285/289 of 1iinA

query
sites
1iinA

K

K

G

G

I

I

L
|
L

A

A

G
|
G

S

S

G

G

T

T

R
|
R

L

L

Y

Y

P

P

L

V

T

T

I

M

A

A

V

V

S

S

K

Q

Q

Q

L

L

M

L

P

P

V

I

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

M

L

A

A

G

G

I

I

R

R

E

D

I

I

M

L

I

I

T

S

T

T

P

P

E

Q

D

D

A

T

D

P

A

R

F

F

K

Q

L

L

G

G

D

D

G

G

S

S

H

Q

L

W

G

G

C

L

E

N

F

L

T

Q

F

Y

A

K

I

V

Q
|
Q

P

P

K

S

P
|
P

E
x
D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

V

I

I

I

G

G

E

E

F

F

I

I

G

G

D

H

D

D

K

D

V

C

A

A

L

L

I

V

L
|
L

G

G

D
|
D

N

N

I

I

F

F

Y

Y

G

G

S

H

G

D

L

L

Q

P

E

K

T

L

L

M

L

E

K

A

H

A

T

V

D

N

P

K

E

E

-

S

G

G

G

A

V

T

V

V

F

F

A

A

Y

Y

H

H

V

V

N

N

D

D

P

P

Q

E

R

R

Y
|
Y

G
|
G

V

V

E

E

F

F

D

D

Q

Q

N

K

K

G

K

T

A

A

I

V

S

S

I

L

E

E

E
|
E

K
|
K

P

P

Q

L

A

Q

P

P

K

K

S

S

N

N

F

Y

A

A

V
|
V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

R

S

V

V

E

E

I

M

A

A

K

K

Q

N

I

L

K

K

P

P

S

S

A

A

R
|
R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

I

N

N

N

R

A

I

Y

Y

L

M

K

E

E

Q

G

G

K

R

L

L

S

S

V

V

G

A

I

M

L

M

S

G

R

R

G

G

T

Y

A

A

W
|
W

L

L

D

D

T

T

G

G

T

T

H

H

Q

Q

S

S

L

L

Q

I

Q

E

A

A

G

S

Q

N

F

F

V

I

E

A

V

T

I

I

E

E

R

R

Q

Q

G

G

L

L

K

K

I

V

G

S

C

C

V

P

E

E

V

I

A

A

Y

F

H

R

K

K

G

N

F

F

I

I

T

N

K

A

Q

Q

L

V

L

I

A

E

Q

L

A

A

E

G

K

P

L

L

G

S

K

K

S

N

G

D

Y

Y

G

G

T

K

Y

Y

L

L

binding uridine-5'-diphosphate-glucose: L9 (= L6), G11 (= G8), G12 (= G9), R16 (= R13), Q83 (= Q80), P86 (= P83), D87 (≠ E84), G88 (= G85), L89 (= L86), L109 (= L106), D111 (= D108), Y146 (= Y142), G147 (= G143), E162 (= E158), K163 (= K159), V173 (= V169), R195 (= R191), W224 (= W220)

1iimA Thymidylyltransferase complexed with ttp (see paper)
65% identity, 98% coverage: 2:281/285 of query aligns to 5:285/289 of 1iimA

query
sites
1iimA

K

K

G

G

I

I

L
|
L

A

A

G
|
G

S
|
S

G
|
G

T
|
T

R
|
R

L

L

Y

Y

P

P

L

V

T

T

I

M

A

A

V

V

S

S

K

Q

Q
|
Q

L

L

M

L

P

P

V

I

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

M

L

A

A

G

G

I

I

R

R

E

D

I

I

M

L

I

I

T

S

T

T

P

P

E

Q

D

D

A

T

D

P

A

R

F

F

K

Q

L

L

G

G

D

D

G

G

S

S

H

Q

L

W

G

G

C

L

E

N

F

L

T

Q

F

Y

A

K

I

V

Q
|
Q

P

P

K

S

P
|
P

E

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

V

I

I

I

G

G

E

E

F

F

I

I

G

G

D

H

D

D

K

D

V

C

A

A

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

S
x
H

G

D

L

L

Q
x
P

E

K

T

L

L

M

L

E

K

A

H

A

T

V

D

N

P

K

E

E

-

S

G

G

G

A

V

T

V

V

F

F

A

A

Y

Y

H

H

V

V

N

N

D

D

P

P

Q

E

R

R

Y

Y

G

G

V

V

E

E

F

F

D

D

Q

Q

N

K

K

G

K

T

A

A

I

V

S

S

I

L

E

E

K

K

P

P

Q

L

A

Q

P

P

K

K

S

S

N

N

F

Y

A

A

V

V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

R

S

V

V

E

E

I

M

A

A

K

K

Q

N

I

L

K

K

P

P

S

S

A

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

I

N

N

N

R

A

I

Y

Y

L

M

K

E

E

Q

G

G

K

R

L

L

S

S

V

V

G

A

I

M

L

M

S
x
G

R
|
R

G
|
G

T

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

Q

Q

S

S

L

L

Q

I

Q

E

A

A

G

S

Q

N

F

F

V

I

E

A

V

T

I

I

E

E

R

R

Q

Q

G

G

L

L

K

K

I

V

G
x
S

C

C

V

P

E

E

E
|
E

V
x
I

A

A

Y

F

H

R

K

K

G

N

F

F

I

I

T

N

K

A

Q

Q

L

V

L

I

A

E

Q

L

A

A

E

G

K

P

L

L

G

S

K

K

S

N

G

D

Y

Y

G

G

T

K

Y

Y

L

L

binding thymidine-5'-triphosphate: L9 (= L6), G11 (= G8), G12 (= G9), S13 (= S10), G14 (= G11), T15 (= T12), R16 (= R13), Q27 (= Q24), Q83 (= Q80), P86 (= P83), G88 (= G85), L89 (= L86), D111 (= D108), Y115 (= Y112), G116 (= G113), H117 (≠ S114), P120 (≠ Q117), G219 (≠ S215), R220 (= R216), G221 (= G217), S252 (≠ G248), E256 (= E252), I257 (≠ V253)

3pkpA Q83s variant of s. Enterica rmla with datp (see paper)
65% identity, 98% coverage: 2:281/285 of query aligns to 5:285/290 of 3pkpA

query
sites
3pkpA

K

K

G

G

I

I

L

L

A

A

G
|
G

S
|
S

G
|
G

T
|
T

R
|
R

L

L

Y

Y

P

P

L

V

T

T

I

M

A

A

V

V

S

S

K
|
K

Q
|
Q

L

L

M

L

P

P

V

I

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

M

L

A

A

G

G

I

I

R

R

E

D

I

I

M

L

I

I

T

S

T

T

P

P

E

Q

D

D

A

T

D

P

A

R

F

F

K

Q

L

L

G

G

D

D

G

G

S

S

H

Q

L

W

G

G

C

L

E

N

F

L

T

Q

F

Y

A

K

I

V

Q
x
S

P

P

K

S

P
|
P

E

D

G

G

L

L

A

A

Q

Q

A

A

F

F

V

I

I

I

G

G

E

E

F

F

I

I

G

G

D

H

D

D

K

D

V

C

A

A

L

L

I

V

L

L

G
|
G

D
|
D

N

N

I

I

F

F

Y

Y

G

G

S

H

G

D

L

L

Q

P

E

K

T

L

L

M

L

E

K

A

H

A

T

V

D

N

P

K

E

E

-

S

G

G

G

A

V

T

V

V

F

F

A

A

Y

Y

H

H

V

V

N

N

D

D

P

P

Q

E

R

R

Y

Y

G

G

V

V

E

E

F

F

D

D

Q

Q

N

K

K

G

K

T

A

A

I

V

S

S

I

L

E

E

K

K

P

P

Q

L

A

Q

P

P

K

K

S

S

N

N

F

Y

A

A

V

V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

R

S

V

V

E

E

I

M

A

A

K

K

Q

N

I

L

K

K

P

P

S

S

A

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

I

N

N

N

R

A

I

Y

Y

L

M

K

E

E

Q

G

G

K

R

L

L

S

S

V

V

G

A

I

M

L

M

S

G

R

R

G

G

T

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

Q

Q

S

S

L

L

Q

I

Q

E

A

A

G

S

Q

N

F

F

V

I

E

A

V

T

I

I

E

E

R

R

Q

Q

G

G

L

L

K

K

I

V

G

S

C

C

V

P

E

E

V

I

A

A

Y

F

H

R

K

K

G

N

F

F

I

I

T

N

K

A

Q

Q

L

V

L

I

A

E

Q

L

A

A

E

G

K

P

L

L

G

S

K

K

S

N

G

D

Y

Y

G

G

T

K

Y

Y

L

L

binding 2'-deoxyadenosine 5'-triphosphate: G11 (= G8), G12 (= G9), S13 (= S10), G14 (= G11), T15 (= T12), R16 (= R13), K26 (= K23), Q27 (= Q24), S83 (≠ Q80), P86 (= P83), G110 (= G107), D111 (= D108)

3pkpB Q83s variant of s. Enterica rmla with datp (see paper)
65% identity, 98% coverage: 2:281/285 of query aligns to 4:284/289 of 3pkpB

query
sites
3pkpB

K

K

G

G

I

I

L

L

A

A

G
|
G

S
|
S

G
|
G

T
|
T

R
|
R

L

L

Y

Y

P

P

L

V

T

T

I

M

A

A

V

V

S

S

K
|
K

Q
|
Q

L

L

M

L

P

P

V

I

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

M

L

A

A

G

G

I

I

R

R

E

D

I

I

M

L

I

I

T

S

T

T

P

P

E

Q

D

D

A

T

D

P

A

R

F

F

K

Q

L

L

G

G

D

D

G

G

S

S

H

Q

L

W

G

G

C

L

E

N

F

L

T

Q

F

Y

A

K

I

V

Q
x
S

P

P

K

S

P
|
P

E

D

G

G

L

L

A

A

Q

Q

A

A

F

F

V

I

I

I

G

G

E

E

F

F

I

I

G

G

D

H

D

D

K

D

V

C

A

A

L

L

I

V

L

L

G
|
G

D
|
D

N

N

I

I

F

F

Y

Y

G

G

S

H

G

D

L

L

Q

P

E

K

T

L

L

M

L

E

K

A

H

A

T

V

D

N

P

K

E

E

-

S

G

G

G

A

V

T

V

V

F

F

A

A

Y

Y

H

H

V

V

N

N

D

D

P

P

Q

E

R

R

Y

Y

G

G

V

V

E

E

F

F

D

D

Q

Q

N

K

K

G

K

T

A

A

I

V

S

S

I

L

E

E

K

K

P

P

Q

L

A

Q

P

P

K

K

S

S

N

N

F

Y

A

A

V

V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

R

S

V

V

E

E

I

M

A

A

K

K

Q

N

I

L

K

K

P

P

S

S

A

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

I

N

N

N

R

A

I

Y

Y

L

M

K

E

E

Q

G

G

K

R

L

L

S

S

V

V

G

A

I

M

L

M

S

G

R

R

G

G

T

Y

A

A

W

W

L

L

D
|
D

T

T

G

G

T

T

H

H

Q

Q

S

S

L

L

Q

I

Q

E

A

A

G

S

Q

N

F

F

V

I

E

A

V

T

I

I

E

E

R

R

Q

Q

G

G

L

L

K

K

I

V

G

S

C

C

V

P

E

E

V

I

A

A

Y

F

H

R

K

K

G

N

F

F

I

I

T

N

K

A

Q

Q

L

V

L

I

A

E

Q

L

A

A

E

G

K

P

L

L

G

S

K

K

S

N

G

D

Y

Y

G

G

T

K

Y

Y

L

L

binding 2'-deoxyadenosine 5'-triphosphate: G10 (= G8), G11 (= G9), S12 (= S10), G13 (= G11), T14 (= T12), R15 (= R13), K25 (= K23), Q26 (= Q24), S82 (≠ Q80), P85 (= P83), G109 (= G107), D110 (= D108)
binding magnesium ion: D110 (= D108), D225 (= D222)

1h5sB Thymidylyltransferase complexed with tmp (see paper)
65% identity, 98% coverage: 2:281/285 of query aligns to 5:285/291 of 1h5sB

query
sites
1h5sB

K

K

G

G

I

I

L
|
L

A

A

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

V

T

T

I

M

A

A

V

V

S

S

K

K

Q

Q

L

L

M

L

P

P

V

I

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

M

L

A

A

G

G

I

I

R

R

E

D

I

I

M

L

I

I

T

S

T

T

P

P

E

Q

D

D

A

T

D

P

A

R

F

F

K

Q

L

L

G

G

D

D

G

G

S

S

H

Q

L

W

G

G

C

L

E

N

F

L

T

Q

F

Y

A

K

I

V

Q
|
Q

P

P

K

S

P
|
P

E
x
D

G
|
G

L

L

A

A

Q

Q

A

A

F

F

V

I

I

I

G

G

E

E

F

F

I

I

G

G

D

G

D

D

K

D

V

C

A

A

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

S
x
H

G
x
D

L

L

Q

P

E

K

T

L

L

M

L

E

K

A

H

A

T

V

D

N

P

K

E

E

-

S

G

G

G

A

V

T

V

V

F

F

A

A

Y

Y

H

H

V

V

N

N

D

D

P

P

Q

E

R

R

Y

Y

G

G

V

V

E

E

F

F

D

D

Q

K

N

N

K

G

K

T

A

A

I

I

S

S

I

L

E

E

K

K

P

P

Q

L

A

E

P

P

K

K

S

S

N

N

F

Y

A

A

V

V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

R

D

V

V

E

Q

I

M

A

A

K

K

Q

N

I

L

K

K

P

P

S

S

A

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

I

N

N

N

R

A

I

Y

Y

L

L

K

E

E

Q

G

G

K

R

L

L

S

S

V

V

G

A

I

L

L

M

S
x
G

R
|
R

G
|
G

T

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

Q

Q

S

S

L

L

Q

I

Q

E

A

A

G

S

Q

N

F

F

V

I

E

A

V

T

I

I

E

E

R

R

Q

Q

G

G

L

L

K

K

I

V

G
x
S

C

C

V

P

E

E

E
|
E

V
x
I

A

A

Y

F

H
x
R

K

K

G

G

F

F

I

I

T

D

K

V

Q

E

Q

Q

L

V

L

R

A

K

Q

L

A

A

E

V

K

P

L

L

G

I

K

K

S

N

G

N

Y

Y

G

G

T

Q

Y

Y

L

L

binding thymidine-5'-phosphate: L9 (= L6), G11 (= G8), G12 (= G9), Q83 (= Q80), P86 (= P83), D87 (≠ E84), G88 (= G85), D111 (= D108), Y115 (= Y112), G116 (= G113), H117 (≠ S114), D118 (≠ G115), G219 (≠ S215), R220 (= R216), G221 (= G217), S252 (≠ G248), E256 (= E252), I257 (≠ V253), R260 (≠ H256)

1h5tA Thymidylyltransferase complexed with thymidylyldiphosphate-glucose (see paper)
65% identity, 98% coverage: 2:281/285 of query aligns to 4:284/290 of 1h5tA

query
sites
1h5tA

K

K

G

G

I

I

L
|
L

A

A

G
|
G

G

G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

V

T

T

I

M

A

A

V

V

S

S

K

K

Q

Q

L

L

M

L

P

P

V

I

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

M

L

A

A

G

G

I

I

R

R

E

D

I

I

M

L

I

I

T

S

T

T

P

P

E

Q

D

D

A

T

D

P

A

R

F

F

K

Q

L

L

G

G

D

D

G

G

S

S

H

Q

L

W

G

G

C

L

E

N

F

L

T

Q

F

Y

A

K

I

V

Q
|
Q

P

P

K

S

P
|
P

E

D

G
|
G

L

L

A

A

Q

Q

A

A

F

F

V

I

I

I

G

G

E

E

F

F

I

I

G

G

D

G

D

D

K

D

V

C

A

A

L

L

I

V

L
|
L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

S
x
H

G
x
D

L

L

Q

P

E

K

T

L

L

M

L

E

K

A

H

A

T

V

D

N

P

K

E

E

-

S

G

G

G

A

V

T

V

V

F

F

A

A

Y

Y

H

H

V

V

N

N

D

D

P

P

Q

E

R

R

Y
|
Y

G
|
G

V

V

E

E

F

F

D

D

Q

K

N

N

K

G

K

T

A

A

I

I

S

S

I

L

E

E

E
|
E

K
|
K

P

P

Q

L

A

E

P

P

K

K

S

S

N

N

F

Y

A

A

V
|
V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

R

D

V

V

E

Q

I

M

A

A

K

K

Q

N

I

L

K

K

P

P

S

S

A

A

R
|
R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

I

N

N

N

R

A

I

Y

Y

L

L

K

E

E

Q

G

G

K

R

L

L

S

S

V

V

G

A

I

M

L

M

S

G

R
|
R

G
|
G

T

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

Q

Q

S

S

L

L

Q

I

Q

E

A

A

G

S

Q

N

F

F

V

I

E

A

V

T

I

I

E

E

R

R

Q

Q

G

G

L

L

K

K

I
x
V

G
x
S

C

C

V

P

E

E

V

I

A

A

Y

F

H

R

K

K

G

G

F

F

I

I

T

D

K

V

Q

E

Q

Q

L

V

L

R

A

K

Q

L

A

A

E

V

K

P

L

L

G

I

K

K

S

N

G

N

Y

Y

G

G

T

Q

Y

Y

L

L

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: L8 (= L6), G10 (= G8), Q82 (= Q80), P85 (= P83), G87 (= G85), L108 (= L106), D110 (= D108), Y145 (= Y142), G146 (= G143), E161 (= E158), K162 (= K159), V172 (= V169), R194 (= R191)
binding thymidine-5'-diphosphate: Y114 (= Y112), G115 (= G113), H116 (≠ S114), D117 (≠ G115), R219 (= R216), G220 (= G217), V250 (≠ I247), S251 (≠ G248)

1h5rA Thymidylyltransferase complexed with thimidine and glucose-1-phospate (see paper)
65% identity, 98% coverage: 2:281/285 of query aligns to 4:284/290 of 1h5rA

query
sites
1h5rA

K

K

G

G

I

I

L
|
L

A

A

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

V

T

T

I

M

A

A

V

V

S

S

K

K

Q

Q

L

L

M

L

P

P

V

I

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

M

L

A

A

G

G

I

I

R

R

E

D

I

I

M

L

I

I

T

S

T

T

P

P

E

Q

D

D

A

T

D

P

A

R

F

F

K

Q

L

L

G

G

D

D

G

G

S

S

H

Q

L

W

G

G

C

L

E

N

F

L

T

Q

F

Y

A

K

I

V

Q
|
Q

P

P

K

S

P
|
P

E

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

V

I

I

I

G

G

E

E

F

F

I

I

G

G

D

G

D

D

K

D

V

C

A

A

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y
|
Y

G
|
G

S

H

G

D

L

L

Q

P

E

K

T

L

L

M

L

E

K

A

H

A

T

V

D

N

P

K

E

E

-

S

G

G

G

A

V

T

V

V

F

F

A

A

Y

Y

H

H

V

V

N

N

D

D

P

P

Q

E

R

R

Y
|
Y

G
|
G

V

V

E

E

F

F

D

D

Q

K

N

N

K

G

K

T

A

A

I

I

S

S

I

L

E

E

E
|
E

K
|
K

P

P

Q

L

A

E

P

P

K

K

S

S

N

N

F

Y

A

A

V
|
V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

R

D

V

V

E

Q

I

M

A

A

K

K

Q

N

I

L

K

K

P

P

S

S

A

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

I

N

N

N

R

A

I

Y

Y

L

L

K

E

E

Q

G

G

K

R

L

L

S

S

V

V

G

A

I

M

L

M

S

G

R

R

G

G

T

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

Q

Q

S

S

L

L

Q

I

Q

E

A

A

G

S

Q

N

F

F

V

I

E

A

V

T

I

I

E

E

R

R

Q

Q

G

G

L

L

K

K

I
x
V

G
x
S

C

C

V

P

E

E

E
|
E

V
x
I

A

A

Y

F

H

R

K

K

G

G

F

F

I

I

T

D

K

V

Q

E

Q

Q

L

V

L

R

A

K

Q

L

A

A

E

V

K

P

L

L

G

I

K

K

S

N

G

N

Y

Y

G

G

T

Q

Y

Y

L

L

binding 1-O-phosphono-alpha-D-glucopyranose: L88 (= L86), Y145 (= Y142), G146 (= G143), E161 (= E158), K162 (= K159), V172 (= V169)
binding thymidine: L8 (= L6), G10 (= G8), G11 (= G9), Q82 (= Q80), P85 (= P83), G87 (= G85), L88 (= L86), D110 (= D108), Y114 (= Y112), G115 (= G113), V250 (≠ I247), S251 (≠ G248), E255 (= E252), I256 (≠ V253)

4hocA Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with udp-n- acetylglucosamine
65% identity, 100% coverage: 1:284/285 of query aligns to 1:283/286 of 4hocA

query
sites
4hocA

M

M

K

K

G

G

I

I

L
|
L

A

S

G
|
G

S

S

G

G

T

T

R

R

L

L

Y

Y

P

P

L

L

T

T

I

K

A

V

V

V

S

S

K

K

Q

Q

L

L

M

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

V

Y

Y

P

P

L

L

S

S

V

V

L

L

M

M

M

L

A

A

G

G

I

I

R

K

E

D

I

I

M

L

I

I

T

S

T

T

P

P

E

E

D

D

A

T

D

P

A

R

F

F

K

E

K

Q

L

L

G

G

D

G

G

G

S

S

H

E

L

L

G

G

C

I

E

S

F

L

T

S

F

Y

A

A

I

V

Q
|
Q

P

S

K

S

P
|
P

E

D

G
|
G

L
|
L

A

A

Q

Q

A

A

F

F

V

I

I

I

G

G

E

E

F

F

I

I

G

G

D

D

K

N

V

V

A

A

L

L

I

V

L

L

G

G

D
|
D

N

N

I

I

F

F

Y

Y

G

G

S

H

G

G

L

F

Q

T

E

E

T

L

L

L

L

Q

K

R

H

A

T

A

D

N

P

R

E

K

-

S

G

G

G

A

V

T

V

I

F

F

A

G

Y

Y

H

N

V

V

N

K

D

D

P

P

Q

Q

R

R

Y

F

G
|
G

V

V

E

E

F

F

D

D

Q

E

N

K

K

G

K

K

A

V

I

I

S

S

I

I

E

E

E
|
E

K
|
K

P

P

Q

E

A

E

P

P

K

K

S

S

N

S

F

Y

A

A

V
|
V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

R

R

V

V

E

D

I

I

A

A

K

K

Q

N

I

I

K

T

P

P

S

S

A

-

R

-

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

V

N

N

N

K

A

A

Y

Y

L

L

K

E

E

L

G

G

K

E

L

L

S

H

V

V

G

E

I

L

L

L

S

G

R

R

G

G

T

F

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

Q

E

S

S

L

L

Q

L

Q

Q

A

A

G

S

Q

Q

F

F

V

I

E

E

V

T

I

I

E

E

E

K

R

R

Q

Q

G

S

L

L

K

K

I

V

G

A

C

C

V

L

E

E

V

I

A

A

Y

Y

H

R

K

M

G

G

F

Y

I

I

T

S

K

R

Q

E

Q

Q

L

L

L

I

A

K

Q

L

A

A

E

E

K

P

L

L

G

M

K

K

S

N

G

E

Y

Y

G

G

T

Q

Y

Y

L

L

K

M

G

N

I

L

binding uridine-diphosphate-n-acetylglucosamine: L6 (= L6), G8 (= G8), G9 (= G9), Q80 (= Q80), P83 (= P83), G85 (= G85), L86 (= L86), D108 (= D108), G144 (= G143), E159 (= E158), K160 (= K159), V170 (= V169)

3pkqA Q83d variant of s. Enterica rmla with dgtp (see paper)
64% identity, 98% coverage: 2:281/285 of query aligns to 3:280/285 of 3pkqA

query
sites
3pkqA

K

K

G

G

I

I

L
|
L

A

A

G
|
G

S
|
S

G
|
G

T
|
T

R
|
R

L

L

Y

Y

P

P

L

V

T

T

I

M

A

A

V

V

S

S

K

K

Q
|
Q

L

L

M

L

P

P

V

I

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

M

L

A

A

G

G

I

I

R

R

E

D

I

I

M

L

I

I

T

S

T

T

P

P

E

Q

D

D

A

T

D

P

A

R

F

F

K

Q

L

L

G

G

D

D

G

G

S

S

H

Q

L

W

G

G

C

L

E

N

F

L

T

Q

F

Y

A

K

I

V

Q
x
D

P

P

K
x
S

P

P

E

D

G

G

L

L

A

A

Q

Q

A

A

F

F

V

I

I

I

G

G

E

E

F

F

I

I

G

G

D

H

D

D

K

D

V

C

A

A

L

L

I

V

L
|
L

G
|
G

D
|
D

N

N

I

I

F

F

Y

Y

G

G

S

H

G

D

L

L

Q

P

E

K

T

L

L

M

L

E

K

A

H

A

T

V

D

N

P

K

E

E

-

S

G

G

G

A

V

T

V

V

F

F

A

A

Y

Y

H

H

V

V

N

N

D

D

P

P

Q

E

R

R

Y

Y

G

G

V

V

E

E

F

F

D

D

Q

-

N

-

K

-

K

T

A

A

I

V

S

S

I

L

E

E

K

K

P

P

Q

L

A

Q

P

P

K

K

S

S

N

N

F

Y

A

A

V

V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

N

R

S

V

V

E

E

I

M

A

A

K

K

Q

N

I

L

K

K

P

P

S

S

A

A

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

I

N

N

N

R

A

I

Y

Y

L

M

K

E

E

Q

G

G

K

R

L

L

S

S

V

V

G

A

I

M

L

M

S

G

R

R

G

G

T

Y

A

A

W

W

L

L

D
|
D

T

T

G

G

T

T

H

H

Q

Q

S

S

L

L

Q

I

Q

E

A

A

G

S

Q

N

F

F

V

I

E

A

V

T

I

I

E

E

R

R

Q

Q

G

G

L

L

K

K

I

V

G

S

C

C

V

P

E

E

V

I

A

A

Y

F

H

R

K

K

G

N

F

F

I

I

T

N

K

A

Q

Q

L

V

L

I

A

E

Q

L

A

A

E

G

K

P

L

L

G

S

K

K

S

N

G

D

Y

Y

G

G

T

K

Y

Y

L

L

binding 2'-deoxyguanosine-5'-triphosphate: L7 (= L6), G9 (= G8), G10 (= G9), S11 (= S10), G12 (= G11), T13 (= T12), R14 (= R13), Q25 (= Q24), D81 (≠ Q80), S83 (≠ K82), L107 (= L106), G108 (= G107), D109 (= D108)
binding magnesium ion: D109 (= D108), D221 (= D222)

4b2xB Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
64% identity, 100% coverage: 2:285/285 of query aligns to 13:297/302 of 4b2xB

query
sites
4b2xB

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

Y

H

P

P

L

A

T

T

I

L

A

A

V

I

S

S

K

K

Q

Q

L

L

M

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

M
x
L

A

A

G

G

I

I

R

R

E

E

I

I

M

L

I

I

T

S

T

T

P

P

E

Q

D

D

A

T

D

P

A

R

F

F

K

Q

L

L

G

G

D

D

G

G

S

S

H

N

L

W

G

G

C

L

E

D

F

L

T

Q

F

Y

A

A

I

V

Q

Q

P

P

K

S

P

P

E

D

G

G

L

L

A

A

Q

Q

A

A

F

F

V

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

K

L

V

S

A

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

Y

Y

G

G

S

H

G

D

L

F

Q

H

E

E

T

L

L

L

L

G

K

S

H

A

T

S

D

Q

P

R

E

Q

-

T

G

G

G

A

V

S

V

V

F

F

A

A

Y

Y

H

H

V

V

N

L

D

D

P

P

Q

E

R

R

Y

Y

G

G

V

V

E

E

F

F

D

D

Q

Q

N

G

K

G

K

K

A

A

I

I

S

S

I

L

E

E

K

K

P

P

Q

L

A

E

P

P

K

K

S

S

N

N

F

Y

A

A

V

V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

R

Q

V

V

E

D

I

I

A

A

K

R

Q

D

I

L

K

K

P

P

S

S

A

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

E

R

G

G

K

Q

L

L

S

S

V

V

G

E

I

I

L

M

S

G

R

R

G

G

T

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

Q

D

S

S

L

L

Q

L

Q

E

A

A

G

G

Q

Q

F

F

V

I

E

A

V

T

I

L

E

E

E

N

R

R

Q

Q

G

G

L

L

K

K

I

V

G

A

C

C

V

P

E

E

E
|
E

V

I

A

A

Y

Y

H

R

K
x
Q

G

K

F

W

I

I

T

D

K

A

Q

A

Q

Q

L

L

L

E

A

K

Q

L

A

A

E

A

K

P

L

L

G

A

K

K

S

N

G

G

Y

Y

G

G

T

Q

Y

Y

L

L

K

K

G

R

I

L

binding 6-amino-1-butyl-5-(cyclopentylamino)pyrimidine-2,4(1H,3H)-dione: L54 (≠ M43), Y122 (≠ F111), E264 (= E252), Q269 (≠ K257)

4b5bA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
64% identity, 100% coverage: 2:285/285 of query aligns to 4:288/293 of 4b5bA

query
sites
4b5bA

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

Y

H

P

P

L

A

T

T

I

L

A

A

V

I

S

S

K

K

Q

Q

L

L

M

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S
|
S

V

T

L

L

M

M

M
x
L

A

A

G

G

I

I

R

R

E

E

I

I

M

L

I

I

T

S

T

T

P

P

E

Q

D

D

A

T

D

P

A

R

F

F

K

Q

L

L

G

G

D

D

G

G

S

S

H

N

L

W

G

G

C

L

E

D

F

L

T

Q

F

Y

A

A

I

V

Q

Q

P

P

K

S

P

P

E

D

G

G

L

L

A

A

Q

Q

A

A

F

F

V

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

K

L

V

S

A

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

Y

Y

G
|
G

S

H

G

D

L

F

Q

H

E

E

T

L

L

L

L

G

K

S

H

A

T

S

D

Q

P

R

E

Q

-

T

G

G

G

A

V

S

V

V

F

F

A

A

Y

Y

H

H

V

V

N

L

D

D

P

P

Q

E

R

R

Y

Y

G

G

V

V

E

E

F

F

D

D

Q

Q

N

G

K

G

K

K

A

A

I

I

S

S

I

L

E

E

K

K

P

P

Q

L

A

E

P

P

K

K

S

S

N

N

F

Y

A

A

V

V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

R

Q

V

V

E

D

I

I

A

A

K

R

Q

D

I

L

K

K

P

P

S

S

A

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

E

R

G

G

K

Q

L

L

S

S

V

V

G

E

I

I

L

M

S

G

R

R

G

G

T

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

Q

D

S

S

L

L

Q

L

Q

E

A

A

G

G

Q

Q

F

F

V

I

E

A

V

T

I

L

E

E

E

N

R

R

Q

Q

G

G

L

L

K

K

I
x
V

G
x
A

C

C

V

P

E

E

E
|
E

V
x
I

A

A

Y

Y

H

R

K

Q

G

K

F

W

I

I

T

D

K

A

Q

A

Q

Q

L

L

L

E

A

K

Q

L

A

A

E

A

K

P

L

L

G

A

K

K

S

N

G

G

Y

Y

G

G

T

Q

Y

Y

L

L

K

K

G

R

I

L

binding N-(6-amino-1-benzyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-N,3-dimethylbenzenesulfonamide: S41 (= S39), L45 (≠ M43), Y113 (≠ F111), G115 (= G113), V250 (≠ I247), A251 (≠ G248), E255 (= E252), I256 (≠ V253)

4b4gA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
64% identity, 100% coverage: 2:285/285 of query aligns to 4:288/293 of 4b4gA

query
sites
4b4gA

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

Y

H

P

P

L

A

T

T

I

L

A

A

V

I

S

S

K

K

Q

Q

L

L

M

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

M
x
L

A

A

G

G

I

I

R

R

E

E

I

I

M

L

I

I

T

S

T

T

P

P

E

Q

D

D

A

T

D

P

A

R

F

F

K

Q

L

L

G

G

D

D

G

G

S

S

H

N

L

W

G

G

C

L

E

D

F

L

T

Q

F

Y

A

A

I

V

Q

Q

P

P

K

S

P

P

E

D

G

G

L

L

A

A

Q

Q

A

A

F

F

V

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

K

L

V

S

A

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

Y

Y

G
|
G

S

H

G

D

L
x
F

Q

H

E

E

T

L

L

L

L

G

K

S

H

A

T

S

D

Q

P

R

E

Q

-

T

G

G

G

A

V

S

V

V

F

F

A

A

Y

Y

H

H

V

V

N

L

D

D

P

P

Q

E

R

R

Y

Y

G

G

V

V

E

E

F

F

D

D

Q

Q

N

G

K

G

K

K

A

A

I

I

S

S

I

L

E

E

K

K

P

P

Q

L

A

E

P

P

K

K

S

S

N

N

F

Y

A

A

V

V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

R

Q

V

V

E

D

I

I

A

A

K

R

Q

D

I

L

K

K

P

P

S

S

A

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

E

R

G

G

K

Q

L

L

S

S

V

V

G

E

I

I

L

M

S

G

R

R

G

G

T

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

Q

D

S

S

L

L

Q

L

Q

E

A

A

G

G

Q

Q

F

F

V

I

E

A

V

T

I

L

E

E

E

N

R

R

Q

Q

G

G

L

L

K

K

I
x
V

G
x
A

C

C

V

P

E

E

E
|
E

V
x
I

A

A

Y

Y

H
x
R

K

Q

G

K

F

W

I

I

T

D

K

A

Q

A

Q

Q

L

L

L

E

A

K

Q

L

A

A

E

A

K

P

L

L

G

A

K

K

S

N

G

G

Y

Y

G

G

T

Q

Y

Y

L

L

K

K

G

R

I

L

binding N-(6-amino-1-benzyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-N-methylbutane-1-sulfonamide: L45 (≠ M43), Y113 (≠ F111), G115 (= G113), F118 (≠ L116), V250 (≠ I247), A251 (≠ G248), E255 (= E252), I256 (≠ V253), R259 (≠ H256)

4b42A Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
64% identity, 100% coverage: 2:285/285 of query aligns to 4:288/293 of 4b42A

query
sites
4b42A

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

Y

H

P

P

L

A

T

T

I

L

A

A

V

I

S

S

K

K

Q

Q

L

L

M

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S
|
S

V
x
T

L

L

M

M

M

L

A

A

G

G

I

I

R

R

E

E

I

I

M

L

I

I

T

S

T

T

P

P

E

Q

D

D

A

T

D

P

A

R

F

F

K

Q

L

L

G

G

D

D

G

G

S

S

H

N

L

W

G

G

C

L

E

D

F

L

T

Q

F

Y

A

A

I

V

Q

Q

P

P

K

S

P

P

E

D

G

G

L

L

A

A

Q

Q

A

A

F

F

V

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

K

L

V

S

A

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F
x
Y

Y

Y

G

G

S

H

G

D

L
x
F

Q

H

E

E

T

L

L

L

L

G

K

S

H

A

T

S

D

Q

P

R

E

Q

-

T

G

G

G

A

V

S

V

V

F

F

A

A

Y

Y

H

H

V

V

N

L

D

D

P

P

Q

E

R

R

Y

Y

G

G

V

V

E

E

F

F

D

D

Q

Q

N

G

K

G

K

K

A

A

I

I

S

S

I

L

E

E

K

K

P

P

Q

L

A

E

P

P

K

K

S

S

N

N

F

Y

A

A

V

V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

R

Q

V

V

E

D

I

I

A

A

K

R

Q

D

I

L

K

K

P

P

S

S

A

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

E

R

G

G

K

Q

L

L

S

S

V

V

G

E

I

I

L

M

S

G

R

R

G

G

T

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

Q

D

S

S

L

L

Q

L

Q

E

A

A

G

G

Q

Q

F

F

V

I

E

A

V

T

I

L

E

E

E

N

R

R

Q

Q

G

G

L

L

K

K

I
x
V

G

A

C

C

V

P

E

E

E
|
E

V
x
I

A

A

Y

Y

H

R

K

Q

G

K

F

W

I

I

T

D

K

A

Q

A

Q

Q

L

L

L

E

A

K

Q

L

A

A

E

A

K

P

L

L

G

A

K

K

S

N

G

G

Y

Y

G

G

T

Q

Y

Y

L

L

K

K

G

R

I

L

binding N-[6-azanyl-1-butyl-2,4-bis(oxidanylidene)pyrimidin-5-yl]-N-methyl-benzamide: S41 (= S39), T42 (≠ V40), Y113 (≠ F111), F118 (≠ L116), V250 (≠ I247), E255 (= E252), I256 (≠ V253)

4b3uA Pseudomonas aeruginosa rmla in complex with allosteric inhibitor (see paper)
64% identity, 100% coverage: 2:285/285 of query aligns to 10:294/299 of 4b3uA

query
sites
4b3uA

K

K

G

G

I

I

L

L

A

A

G

G

S

S

G

G

T

T

R

R

L

L

Y

H

P

P

L

A

T

T

I

L

A

A

V

I

S

S

K

K

Q

Q

L

L

M

L

P

P

V

V

Y

Y

D

D

K

K

P

P

M

M

I

I

Y

Y

P

P

L

L

S

S

V

T

L

L

M

M

M
x
L

A

A

G

G

I

I

R

R

E

E

I

I

M

L

I

I

T

S

T

T

P

P

E

Q

D

D

A

T

D

P

A

R

F

F

K

Q

L

L

G

G

D

D

G

G

S

S

H

N

L

W

G

G

C

L

E

D

F

L

T

Q

F

Y

A

A

I

V

Q

Q

P

P

K

S

P

P

E

D

G

G

L

L

A

A

Q

Q

A

A

F

F

V

L

I

I

G

G

E

E

E

S

F

F

I

I

G

G

D

N

D

D

K

L

V

S

A

A

L

L

I

V

L

L

G

G

D

D

N

N

I

L

F

Y

Y

Y

G

G

S

H

G

D

L

F

Q

H

E

E

T

L

L

L

L

G

K

S

H

A

T

S

D

Q

P

R

E

Q

-

T

G

G

G

A

V

S

V

V

F

F

A

A

Y

Y

H

H

V

V

N

L

D

D

P

P

Q

E

R

R

Y

Y

G

G

V

V

E

E

F

F

D

D

Q

Q

N

G

K

G

K

K

A

A

I

I

S

S

I

L

E

E

K

K

P

P

Q

L

A

E

P

P

K

K

S

S

N

N

F

Y

A

A

V

V

P

T

G

G

L

L

Y

Y

F

F

Y

Y

D

D

N

Q

R

Q

V

V

E

D

I

I

A

A

K

R

Q

D

I

L

K

K

P

P

S

S

A

P

R

R

G

G

E

E

L

L

E

E

I

I

T

T

D

D

V

V

N

N

N

R

A

A

Y

Y

L

L

K

E

E

R

G

G

K

Q

L

L

S

S

V

V

G

E

I

I

L

M

S

G

R

R

G

G

T

Y

A

A

W

W

L

L

D

D

T

T

G

G

T

T

H

H

Q

D

S

S

L

L

Q

L

Q

E

A

A

G

G

Q

Q

F

F

V

I

E

A

V

T

I

L

E

E

E

N

R

R

Q

Q

G

G

L

L

K

K

I

V

G

A

C

C

V

P

E

E

E
|
E

V
x
I

A

A

Y

Y

H
x
R

K
x
Q

G

K

F

W

I

I

T

D

K

A

Q

A

Q

Q

L

L

L

E

A

K

Q

L

A

A

E

A

K

P

L

L

G

A

K

K

S

N

G

G

Y

Y

G

G

T

Q

Y

Y

L

L

K

K

G

R

I

L

binding 6-amino-1-benzyl-5-(ethylamino)pyrimidine-2,4(1H,3H)-dione: L51 (≠ M43), E261 (= E252), I262 (≠ V253), R265 (≠ H256), Q266 (≠ K257)

Query Sequence

>Echvi_2361 FitnessBrowser__Cola:Echvi_2361
MKGIILAGGSGTRLYPLTIAVSKQLMPVYDKPMIYYPLSVLMMAGIREIMIITTPEDADA
FKKLLGDGSHLGCEFTFAIQPKPEGLAQAFVIGEEFIGDDKVALILGDNIFYGSGLQETL
LKHTDPEGGVVFAYHVNDPQRYGVVEFDQNKKAISIEEKPQAPKSNFAVPGLYFYDNRVV
EIAKQIKPSARGELEITDVNNAYLKEGKLSVGILSRGTAWLDTGTHQSLQQAGQFVEVIE
ERQGLKIGCVEEVAYHKGFITKQQLLAQAEKLGKSGYGTYLKGII

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory