SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_2634 FitnessBrowser__Cola:Echvi_2634 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 16 hits to proteins with known functional sites (download)

P0A7B5 Glutamate 5-kinase; Gamma-glutamyl kinase; GK; EC 2.7.2.11 from Escherichia coli (strain K12) (see paper)
36% identity, 93% coverage: 14:263/268 of query aligns to 5:253/367 of P0A7B5

query
sites
P0A7B5

K

Q

R

T

I

L

V

V

I

V

K

K

V

L

G

G

T

T

N

S

V

V

M

L

T

T

N

G

R

G

D

S

N

R

R

R

I

L

V

N

N

R

T

A

V

H

L

I

R

V

K

E

M

L

V

V

D

R

Q

Q

I

C

A

A

I

Q

L

L

Y

H

E

A

R

A

G

G

I

H

M

R

S

I

V

V

L

I

V

V

S

T

S
|
S

G

G

S

A

V

I

I

A

A

A

G

G

K

R

E

E

V

H

L

L

G

G

S

Y

K

P

V

E

G

-

I

L

K

P

D

A

K

T

I

I

I

A

R

S

R

K

Q

Q

V

L

F

L

S

A

A

A

V

V

G

G

Q

Q

P

S

R

R

M

L

M

I

R

Q

H

L

Y

W

Y

E

N

Q

I

L

F

F

Q

S

D

I

Y

Y

G

G

M

I

R

H

C

V

A

G

Q

Q

V

M

L

L

A

L

T

T

K

R

R

A

D

D

F

M

D

E

P

D

G

R

K

E

H

R

R

F

E

L

N

N

M

A

I

R

N

D

C

T

Y

L

E

R

G

A

L

L

L

L

S

D

E

N

G

N

I

I

I

V

P

P

I

V

A

I

N

N

E

E

D

N

D
|
D

A

A

V

V

S

A

L

T

S

A

M

E

S

I

T

K

F

V

T

G

D

D

N
|
N

D

D

E

N

L

L

A

S

S

A

L

L

V

A

A

A

E

I

L

L

T

A

K

G

A

A

D

D

M

K

L

L

I

L

L

L

L

L

T

T

D

D

T

Q

D

K

G

G

L

L

Y

Y

N

T

G

A

H

D

P

P

-

R

D

S

D

N

E

P

N

Q

T

A

E

E

R

L

I

I

A

K

H

D

V

V

-

Y

G

G

T

I

D

D

E

D

K

A

V

L

E

R

H

A

F

I

I

A

Q

G

E

D

S

S

T

V

K

S

G

G

E

-

A

-

E

L

G

G

R

T

G

G

G

G

M

M

K

S

S

T

K

K

L

L

N

Q

V

A

A

A

K

D

E

V

A

A

A

C

R

R

K

A

N

G

I

I

P

D

T

T

Y

I

I

I

A

A

N

A

G

G

N

S

V

K

D

P

N

G

M

V

I

I

L

G

D

D

I

V

V

M

D

E

G

G

K

I

E

S

V

V

G

G

T

T

S50 (= S59) binding
D137 (= D147) binding
N149 (= N159) binding

2j5tD Glutamate 5-kinase from escherichia coli complexed with glutamate (see paper)
35% identity, 93% coverage: 14:263/268 of query aligns to 3:251/365 of 2j5tD

query
sites
2j5tD

K

Q

R

T

I

L

V

V

I

V

K

K

V

L

G

G

T

T

N

S

V

V

M

L

T

T

N

G

R

G

D

S

N

R

R

R

I

L

V

N

N

R

T

A

V

H

L

I

R

V

K

E

M

L

V

V

D

R

Q

Q

I

C

A

A

I

Q

L

L

Y

H

E

A

R

A

G

G

I

H

M

R

S

I

V

V

L

I

V

V

S

T

S
|
S

G
|
G

S
x
A

V

I

I

A

A

A

G

G

K

R

E

E

V

H

L

L

G

G

S

Y

K

P

V

E

G

-

I

L

K

P

D

A

K

T

I

I

I

A

R

S

R

K

Q

Q

V

L

F

L

S

A

A

A

V

V

G

G

Q

Q

P

S

R

R

M

L

M

I

R

Q

H

L

Y

W

Y

E

N

Q

I

L

F

F

Q

S

D

I

Y

Y

G

G

M

I

R

H

C

V

A

G

Q

Q

V

M

L

L

A

L

T

T

K

R

R

A

D

D

F

M

D

E

P

D

G

R

K

E

H

R

R

F

E

L

N

N

M

A

I

R

N

D

C

T

Y

L

E

R

G

A

L

L

L

L

S

D

E

N

G

N

I

V

I

V

P

P

I

V

A

I

N
|
N

E

E

D

N

D
|
D

A

A

V

V

S

A

L

T

S

A

M

E

S

I

T

K

F

V

T

G

D
|
D

N
|
N

D

D

E

N

L

L

A

S

S

A

L

L

V

A

A

A

E

I

L

L

T

A

K

G

A

A

D

D

M

K

L

L

I

L

L

L

L

L

T

T

D

D

T

Q

D

K

G

G

L

L

Y

Y

N

T

G

A

H

D

P

P

-

R

D

S

D

N

E

P

N

Q

T

A

E

E

R

L

I

I

A

K

H

D

V

V

-

Y

G

G

T

I

D

D

E

D

K

A

V

L

E

R

H

A

F

I

I

A

Q

G

E

D

S

S

T

V

K

S

G

G

E

-

A

-

E

L

G

G

R

T

G

G

G

G

M

M

K

S

S

T

K

K

L

L

N

Q

V

A

A

A

K

D

E

V

A

A

A

C

R

R

K

A

N

G

I

I

P

D

T

T

Y

I

I

I

A

A

N

A

G

G

N

S

V

K

D

P

N

G

M

V

I

I

L

G

D

D

I

V

V

M

D

E

G

G

K

I

E

S

V

V

G

G

T

T

binding glutamic acid: S48 (= S59), G49 (= G60), A50 (≠ S61), N132 (= N144), D135 (= D147), D146 (= D158), N147 (= N159)

Sites not aligning to the query:

binding glutamic acid: 282, 285

7wx3B Gk domain of drosophila p5cs filament with glutamate, atp, and NADPH (see paper)
32% identity, 95% coverage: 14:267/268 of query aligns to 13:258/258 of 7wx3B

query
sites
7wx3B

K

R

R

R

I

L

V

V

I

V

K

K

V

L

G

G

T

S

N

A

V

V

M

I

T

T

N

R

R

E

D

D

N

N

R

H

-

G

I

L

V

A

N

L

T

G

V

R

L

L

R

A

K

S

M

I

V

V

D

E

Q

Q

I

V

A

A

I

E

L

C

Y

H

E

L

R

E

G

G

I

R

M

E

S

V

V

M

L

M

V

V

S

T

S
|
S

G

G

S
x
A

V

V

I

A

A

F

G

G

K

K

E

Q

V

K

L

L

G

A

S

Q

K
x
E

V

L

G

L

I
x
M

K

S

D
x
L

K
x
S

I
x
M

-
x
R

-

E

-

T

-

L

-

N

I

L

R

E

R

P

Q

R

V

A

F

A

S

A

A

A

V

V

G

G

Q

Q

P

S

R

G

M

L

M

M

R

S

H

L

Y

Y

Y

D

N

A

I

M

F

F

Q

A

D

Q

Y

Y

G

G

M

V

R

K

C

I

A

A

Q

Q

V

V

L

L

A

V

T

T

K

K

R

P

D

D

F

F

D

Y

P

N

G

E

K

E

H

T

R

R

E

N

N

N

M

L

I

F

N

C

C

T

Y

L

E

S

G

E

L

L

L

I

S

S

E

L

G

N

I

I

I

V

P

P

I

I

A

I

N
|
N

E
x
T

D

N

D
|
D

A

A

V

V

S

S

L

P

S

P

M
|
M

S

F

-

I

T

P

F

I

T

K

D

D

N
|
N

D

D

E

S

L

L

A

S

S

A

L

M

V

L

A

A

E

A

L

E

T

V

K

Q

A

A

D

D

M

L

L

L

I

I

L

L

L

M

T

S

D

D

T

V

D

D

G

G

L

I

Y

Y

N

N

G

-

H

K

P

P

D

P

D

W

E

E

N

D

T

G

E

A

R

K

I

L

A

M

H

H

V

T

G

Y

T

T

D

S

E

D

K

-

V

-

E

-

H

-

F

-

I

-

Q

-

E

-

S

-

T

-

K

-

G

-

E

-

A

-

E

D

G

S

R

N

G

S

G

I

M

M

K

D

S

S

K

K

L

V

N

K

V

A

A

A

K

T

E

W

A

A

A

L

R

D

K

R

N

G

I

V

P

S

T

V

Y

V

I

I

A

C

N

N

G

G

N

M

V

Q

D

E

N

K

M

A

I

I

L

K

D

T

I

I

V

I

D

G

G

G

K

R

E

K

V

V

G

G

T

T

K

F

V

F

S

T

D

E

binding gamma-glutamyl phosphate: S59 (= S59), A61 (≠ S61), N149 (= N144), T150 (≠ E145), D152 (= D147), M158 (= M153), N165 (= N159)
binding : E72 (≠ K72), M75 (≠ I75), L77 (≠ D77), S78 (≠ K78), M79 (≠ I79), R80 (vs. gap)

7f5xA Gk domain of drosophila p5cs filament with glutamate (see paper)
32% identity, 95% coverage: 14:267/268 of query aligns to 13:236/236 of 7f5xA

query
sites
7f5xA

K

R

R

R

I

L

V

V

I

V

K

K

V

L

G

G

T

S

N

A

V

V

M

I

T

T

N

R

R

E

D

D

N

N

R

H

-

G

I

L

V

A

N

L

T

G

V

R

L

L

R

A

K

S

M

I

V

V

D

E

Q

Q

I

V

A

A

I

E

L

C

Y

H

E

L

R

E

G

G

I

R

M

E

S

V

V

M

L

M

V

V

S

T

S
|
S

G

G

S
x
A

V

V

I

A

A

F

G

G

K

K

E

Q

V

K

L

L

G

A

S

Q

K

E

V

L

G

-

I

L

K

M

D

S

K

L

I

S

I

M

R

R

R

P

Q

R

V

A

F

A

S

A

A

A

V

V

G

G

Q

Q

P

S

R

G

M

L

M

M

R

S

H

L

Y

Y

Y

D

N

A

I

M

F

F

Q

A

D

Q

Y

Y

G

G

M

V

R

K

C

I

A

A

Q

Q

V

V

L

L

A

V

T

T

K

K

R

P

D

D

F

F

D

Y

P

N

G

E

K

E

H

T

R

R

E

N

N

N

M

L

I

F

N

C

C

T

Y

L

E

S

G

E

L

L

L

I

S

S

E

L

G

N

I

I

I

V

P

P

I

I

A

I

N
|
N

E

T

D

N

D
|
D

A

A

V

V

S

S

L

-

S

-

M

-

S

-

T

-

F

-

T

P

D

D

N

N

D

D

E

S

L

L

A

S

S

A

L

M

V

L

A

A

E

A

L

E

T

V

K

Q

A

A

D

D

M

L

L

L

I

I

L

L

L

M

T

S

D

D

T

V

D

D

G

G

L

I

Y

Y

N

N

G

-

H

K

P

P

D

P

D

W

E

E

N

D

T

G

E

A

R

K

I

L

A

M

H

H

V

T

G

Y

T

T

D

-

E

-

K

-

V

-

E

-

H

-

F

-

I

-

Q

-

E

-

S

-

T

-

K

-

G

-

E

-

A

-

E

-

G

-

R

-

G

-

G

-

M

-

K

-

S

S

K

K

L

V

N

K

V

A

A

A

K

T

E

W

A

A

A

L

R

D

K

R

N

G

I

V

P

S

T

V

Y

V

I

I

A

C

N

N

G

G

N

M

V

Q

D

E

N

K

M

A

I

I

L

K

D

T

I

I

V

I

D

G

G

G

K

R

E

K

V

V

G

G

T

T

K

F

V

F

S

T

D

E

binding gamma-l-glutamic acid: S59 (= S59), A61 (≠ S61), N143 (= N144), D146 (= D147)

2akoA Crystal structure of glutamate 5-kinase from campylobacter jejuni
32% identity, 87% coverage: 14:246/268 of query aligns to 1:219/241 of 2akoA

query
sites
2akoA

K

K

R

R

I

I

V

V

I

V

K

K

V

V

G

G

T

S

N
x
H

V

V

M

I

T

S

N

-

R

E

D

E

N

N

R

T

I

L

V

S

N

F

T

E

V

R

L

L

R

K

K

N

M

L

V

V

D

A

Q

F

I

L

A

A

I

K

L

L

Y

M

E

E

R

K

G

-

I

Y

M

E

S

V

V

I

L

L

V

V

S

T

S

S

G

A

S

A

V

I

I

S

A

A

G

G

K

H

E

-

V

-

L

-

G

-

S

T

K

K

V

L

G

D

I

I

K

D

D

R

K

K

-

N

I

L

I

I

R

N

R

K

Q

Q

V

V

F

L

S

A

A

A

V

I

G

G

Q

Q

P

P

R

F

M

L

M

I

R

S

H

V

Y

Y

Y

N

N

E

I

L

F

L

Q

A

D

K

Y

F

G

N

M

K

R

L

C

G

A

G

Q

Q

V

I

L

L

A

L

T

T

K

G

R

K

D

D

F

F

D

D

P

S

G

R

K

K

H

A

R

T

E

K

N

H

M

A

I

K

N

N

C

A

Y

I

E

D

G

M

L

M

L

I

S

N

E

L

G

G

I

I

I

L

P

P

I

I

A

I

N

N

E

E

D

N

D

D

A

A

V

T

S

A

L

I

S

E

M

E

S

I

T

V

F

F

T

G

D

D

N

N

D

D

E

S

L

L

A

S

S

A

L

Y

V

A

A

T

E

H

L

F

T

F

K

D

A

A

D

D

M

L

L

L

I

V

L

I

L

L

T
x
S

D
|
D

T
x
I

D

D

G

G

L
x
F

Y
|
Y

N

D

G

K

H
x
N

P
|
P

D

S

D

E

-

F

E

S

N

D

T

A

E

K

R

R

I

L

A

-

H

-

V

-

G

-

T

-

D

-

E

E

K

K

V

I

E

T

H

H

F

I

I

K

Q

E

E

E

S

W

T

L

K

-

G

-

E

-

A

-

E

H

G

G

R
x
T

G

G

G
|
G

M

I

K

V

S

T

K
|
K

L

L

N

K

V

A

A

A

K

K

E

F

A

L

A

L

R

E

K

H

N

N

I

K

P

K

T

M

Y

F

I

L

A

A

N

S

G

G

binding adenosine-5'-diphosphate: H10 (≠ N23), S161 (≠ T179), D162 (= D180), I163 (≠ T181), F166 (≠ L184), Y167 (= Y185), N170 (≠ H188), P171 (= P189), T194 (≠ R221), G196 (= G223), K200 (= K227)

2j5vB Glutamate 5-kinase from escherichia coli complexed with glutamyl-5- phosphate and pyroglutamic acid (see paper)
31% identity, 93% coverage: 14:263/268 of query aligns to 3:211/325 of 2j5vB

query
sites
2j5vB

K

Q

R

T

I

L

V

V

I

V

K
|
K

V

L

G

G

T
|
T

N

S

V

V

M

L

T

T

N

G

R

G

D

S

N

R

R

R

I

L

V

N

N

R

T

A

V

H

L

I

R

V

K

E

M

L

V

V

D

R

Q

Q

I

C

A

A

I

Q

L

L

Y

H

E

A

R

A

G

G

I

H

M

R

S

I

V

V

L

I

V

V

S

T

S
|
S

G
|
G

S
x
A

V
x
I

I
x
A

A

A

G

G

K

R

E

E

V

H

L

L

G

G

S

Y

K

P

V

E

G

-

I

L

K

P

D

A

K

T

I

I

I

A

R

S

R

K

Q

Q

V

L

F

L

S

A

A

A

V

V

G

G

Q

Q

P

S

R

R

M

L

M

I

R

Q

H

L

Y

W

Y

E

N

Q

I

L

F

F

Q

S

D

I

Y

Y

G

G

M

I

R

H

C

V

A

G

Q

Q

V

M

L

L

A

L

T

T

K

R

R

A

D

D

F

M

D

E

P

D

G

R

K

E

H

R

R

F

E

L

N

N

M

A

I

R

N

D

C

T

Y

L

E

R

G

A

L

L

L

L

S

D

E

N

G

N

I

V

I

V

P

P

I

V

A

I

N

N

E

E

D

N

D
|
D

A

A

V

V

S

A

L

T

S

A

M

E

S

I

T

K

F

V

T
x
G

D
|
D

N
|
N

D

D

E

N

L

L

A

S

S

A

L

L

V

A

A

A

E

I

L

L

T

A

K

G

A

A

D

D

M

K

L

L

I

L

L

L

L

L

T

T

D

D

T

-

D

-

G

-

L

-

Y

-

N

-

G

-

H

-

P

-

D

-

D

-

E

-

N

-

T

-

E

-

R

-

I

-

A

-

H

-

V

-

G

-

T

-

D

-

E

-

K

-

V

-

E

-

H

-

F

-

I

-

Q

-

E

-

S

-

T

-

K

-

G

-

E

-

A

-

E

-

G

-

R

Q

G

G

G

G

M

M

K

S

S

T

K

K

L

L

N

Q

V

A

A

A

K

D

E

V

A

A

A

C

R

R

K

A

N

G

I

I

P

D

T

T

Y

I

I

I

A

A

N

A

G

G

N

S

V

K

D

P

N

G

M

V

I

I

L

G

D

D

I

V

V

M

D

E

G

G

K

I

E

S

V

V

G

G

T

T

binding pyroglutamic acid: T11 (= T22), G49 (= G60), A50 (≠ S61), I51 (≠ V62), A52 (≠ I63), D135 (= D147)
binding gamma-glutamyl phosphate: K8 (= K19), S48 (= S59), D135 (= D147), G145 (≠ T157), D146 (= D158), N147 (= N159)

2j5vA Glutamate 5-kinase from escherichia coli complexed with glutamyl-5- phosphate and pyroglutamic acid (see paper)
30% identity, 93% coverage: 14:263/268 of query aligns to 3:209/323 of 2j5vA

query
sites
2j5vA

K

Q

R

T

I

L

V

V

I

V

K
|
K

V

L

G
|
G

T
|
T

N

S

V

V

M

L

T

T

N

G

R

G

D

S

N

R

R

R

I

L

V

N

N

R

T

A

V

H

L

I

R

V

K

E

M

L

V

V

D

R

Q

Q

I

C

A

A

I

Q

L

L

Y

H

E

A

R

A

G

G

I

H

M

R

S

I

V

V

L

I

V

V

S

T

S
|
S

G
|
G

S
x
A

V
x
I

I

A

A

A

G

G

K

R

E

E

V

H

L

L

G

G

S

Y

K

P

V

-

G

E

I

L

K

P

D

A

K

T

I

I

I

A

R

S

R

K

Q

Q

V

L

F

L

S

A

A

A

V

V

G

G

Q

Q

P

S

R

R

M

L

M

I

R

Q

H

L

Y

W

Y

E

N

Q

I

L

F

F

Q

S

D

I

Y

Y

G

G

M

I

R

H

C

V

A

G

Q

Q

V

M

L

L

A

L

T

T

K

R

R

A

D

D

F

M

D

E

P

D

G

R

K

E

H

R

R

F

E

L

N

N

M

A

I

R

N

D

C

T

Y

L

E

R

G

A

L

L

L

L

S

D

E

N

G

N

I

V

I

V

P

P

I

V

A

I

N

N

E

E

D

N

D
|
D

A

A

V

V

S

A

L

T

S

A

M

E

S

I

T

K

F

V

T

G

D
|
D

N
|
N

D

D

E

N

L

L

A

S

S

A

L

L

V

A

A

A

E

I

L

L

T

A

K

G

A

A

D

D

M

K

L

L

I

L

L

L

L
|
L

T

T

D

D

T

Q

D

-

G

-

L

-

Y

-

N

-

G

-

H

-

P

-

D

-

D

-

E

-

N

-

T

-

E

-

R

-

I

-

A

-

H

-

V

-

G

-

T

-

D

-

E

-

K

-

V

-

E

-

H

-

F

-

I

-

Q

-

E

-

S

-

T

-

K

-

G

-

E

-

A

-

E

-

G

-

R

-

G

-

G

-

M

M

K

S

S

T

K

K

L

L

N

Q

V

A

A

A

K

D

E

V

A

A

A

C

R

R

K

A

N

G

I

I

P

D

T

T

Y

I

I

I

A

A

N

A

G

G

N

S

V

K

D

P

N

G

M

V

I

I

L

G

D

D

I

V

V

M

D

E

G

G

K

I

E

S

V

V

G

G

T

T

binding magnesium ion: K8 (= K19), G10 (= G21), L166 (= L178)
binding pyroglutamic acid: T11 (= T22), S48 (= S59), G49 (= G60), A50 (≠ S61), I51 (≠ V62)
binding gamma-glutamyl phosphate: K8 (= K19), G10 (= G21), S48 (= S59), D135 (= D147), D146 (= D158), N147 (= N159)

8u0mA Isopentenyl phosphate kinase (see paper)
26% identity, 49% coverage: 134:265/268 of query aligns to 123:243/247 of 8u0mA

query
sites
8u0mA

L

L

L

L

S

E

E

L

G

K

I

F

I

I

P

P

I

V

A

L

N

F

E
x
G

D

D

D

T

A

A

V

I

S

A

L

L

S

D

M

K

S

G

T

I

-

D

F
x
I

T

L

D

S

N
x
G

D

D

E

Q

L

I

A

V

S

S

L

Y

V

L

A

A

E

K

L

M

T

L

K

K

A

P

D

S

M

K

L

V

I

I

L

F

L

L

T

M

D

D

T
x
V

D

D

G

G

L

I

Y
|
Y

N

D

G
x
R

H

N

P
|
P

D

K

D

E

E

R

N

D

T

A

E

K

R

L

I

I

A

E

H

E

V

L

G

N

T

V

D

E

E

E

K

-

V

I

E

R

H

H

F

L

I

L

Q

E

E

-

S

-

T

-

K

-

G

-

E

-

A

-

E

-

G

-

R

-

G

S

G
|
G

M
x
I

K

G

S

N

K
|
K

L

L

N

R

V

E

A

A

K

L

E

K

A

I

A

A

R

-

K

K

N

H

I

S

P

E

T

V

Y

Y

I

F

A

I

N

N

G

G

N

K

V

V

D

K

N

E

M

N

I

L

L

G

D

K

I

A

V

I

D

R

G

G

K

E

E

K

V

V

G

G

T

T

K

R

V

L

binding adenosine-5'-diphosphate: V171 (≠ T181), Y175 (= Y185), R177 (≠ G187), P179 (= P189), G202 (= G223), I203 (≠ M224), K206 (= K227)
binding (2E)-2-methylbut-2-en-1-yl dihydrogen phosphate: G134 (≠ E145), I146 (≠ F156), G149 (≠ N159)

Sites not aligning to the query:

8u0nA Isopentenyl phosphate kinase (see paper)
26% identity, 49% coverage: 134:265/268 of query aligns to 122:241/245 of 8u0nA

query
sites
8u0nA

L

L

L

L

S

E

E

L

G

K

I

F

I

I

P

P

I

V

A

L

N

F

E
x
G

D

D

D

T

A

A

V

I

S

A

L

L

S

D

M

K

S

G

T

I

-

D

F
x
I

T

L

D
x
S

N
x
G

D

D

E

Q

L

I

A

V

S

S

L

Y

V

L

A

A

E

K

L

M

T

L

K

K

A

P

D

S

M

K

L

V

I

I

L

F

L

L

T

M

D

D

T

V

D

D

G

G

L
x
I

Y
|
Y

N

D

G
x
R

H

N

P
|
P

D

K

D

E

E

R

N

D

T

A

E

K

R

L

I

I

A

E

H

E

V

L

G

N

T

V

D

E

E

E

K

-

V

I

E

R

H

H

F

L

I

L

Q

-

E

E

S

S

T
x
I

K

G

G

N

E
x
K

A

-

E

-

G

-

R

-

G

-

G

-

M

L

K

R

S

E

K

A

L

L

N

K

V

I

A

A

K

K

E

H

A

S

A

E

R

-

K

-

N

-

I

-

P

-

T

V

Y

Y

I

F

A

I

N

N

G

G

N

K

V

V

D

K

N

E

M

N

I

L

L

G

D

K

I

A

V

I

D

R

G

G

K

E

E

K

V

V

G

G

T

T

K

R

V

L

binding adenosine-5'-diphosphate: I173 (≠ L184), Y174 (= Y185), R176 (≠ G187), P178 (= P189), I201 (≠ T214), K204 (≠ E217)
binding 2-cyclopentylideneethyl dihydrogen phosphate: G133 (≠ E145), I145 (≠ F156), S147 (≠ D158), G148 (≠ N159)

Sites not aligning to the query:

8u0mB Isopentenyl phosphate kinase (see paper)
26% identity, 49% coverage: 134:265/268 of query aligns to 124:243/247 of 8u0mB

query
sites
8u0mB

L

L

L

L

S

E

E

L

G

K

I

F

I

I

P

P

I

V

A

L

N

F

E
x
G

D

D

D

T

A

A

V

I

S

A

L

L

S

D

M

K

S

G

T

I

-

D

F

I

T

L

D

S

N

G

D

D

E

Q

L

I

A

V

S

S

L

Y

V

L

A

A

E

K

L

M

T

L

K

K

A

P

D

S

M

K

L

V

I

I

L

F

L

L

T

M

D

D

T

V

D

D

G

G

L

I

Y
|
Y

N

D

G
x
R

H

N

P
|
P

D

K

D

E

E

R

N

D

T

A

E

K

R

L

I

I

A

E

H

E

V

L

G

N

T

V

D

E

E

E

K

-

V

I

E

R

H

H

F

L

I

L

Q

-

E

E

S

S

T
x
I

K

G

G

N

E
x
K

A

-

E

-

G

-

R

-

G

-

G

-

M

L

K

R

S

E

K

A

L

L

N

K

V

I

A

A

K

K

E

H

A

S

A

E

R

-

K

-

N

-

I

-

P

-

T

V

Y

Y

I

F

A

I

N

N

G

G

N

K

V

V

D

K

N

E

M

N

I

L

L

G

D

K

I

A

V

I

D

R

G

G

K

E

E

K

V

V

G

G

T

T

K

R

V

L

binding adenosine-5'-triphosphate: Y176 (= Y185), R178 (≠ G187), P180 (= P189), I203 (≠ T214), K206 (≠ E217)
binding (2E)-2-methylbut-2-en-1-yl dihydrogen phosphate: G135 (≠ E145)

Sites not aligning to the query:

8u0lA Isopentenyl phosphate kinase (see paper)
26% identity, 49% coverage: 134:265/268 of query aligns to 124:243/247 of 8u0lA

query
sites
8u0lA

L

L

L

L

S

E

E

L

G

K

I

F

I

I

P

P

I

V

A

L

N

F

E
x
G

D

D

D

T

A

A

V

I

S

A

L

L

S

D

M

K

S

G

T

I

-

D

F
x
I

T

L

D

S

N

G

D

D

E

Q

L

I

A

V

S

S

L

Y

V

L

A

A

E

K

L

M

T

L

K

K

A

P

D

S

M

K

L

V

I

I

L

F

L

L

T

M

D

D

T
x
V

D

D

G

G

L

I

Y
|
Y

N

D

G
x
R

H

N

P
|
P

D

K

D

E

E

R

N

D

T

A

E

K

R

L

I

I

A

E

H

E

V

L

G

N

T

V

D

E

E

E

K

-

V

I

E

R

H

H

F

L

I

L

Q

-

E

E

S

S

T
x
I

K

G

G

N

E
x
K

A

-

E

-

G

-

R

-

G

-

G

-

M

L

K

R

S

E

K

A

L

L

N

K

V

I

A

A

K

K

E

H

A

S

A

E

R

-

K

-

N

-

I

-

P

-

T

V

Y

Y

I

F

A

I

N

N

G

G

N

K

V

V

D

K

N

E

M

N

I

L

L

G

D

K

I

A

V

I

D

R

G

G

K

E

E

K

V

V

G

G

T

T

K

R

V

L

binding adenosine-5'-diphosphate: V172 (≠ T181), Y176 (= Y185), R178 (≠ G187), P180 (= P189), I203 (≠ T214), K206 (≠ E217)
binding (2E)-but-2-en-1-yl dihydrogen phosphate: G135 (≠ E145), I147 (≠ F156)

Sites not aligning to the query:

8u0kA Isopentenyl phosphate kinase (see paper)
26% identity, 49% coverage: 134:265/268 of query aligns to 124:243/247 of 8u0kA

query
sites
8u0kA

L

L

L

L

S

E

E

L

G

K

I

F

I

I

P

P

I

V

A

L

N

F

E
x
G

D

D

D

T

A

A

V

I

S

A

L

L

S

D

M

K

S

G

T

I

-

D

F
x
I

T

L

D
x
S

N
x
G

D

D

E

Q

L

I

A

V

S

S

L

Y

V

L

A

A

E

K

L

M

T

L

K

K

A

P

D

S

M

K

L

V

I

I

L

F

L

L

T

M

D

D

T

V

D

D

G

G

L
x
I

Y
|
Y

N

D

G
x
R

H

N

P
|
P

D

K

D

E

E

R

N

D

T

A

E

K

R

L

I

I

A

E

H

E

V

L

G

N

T

V

D

E

E

E

K

-

V

I

E

R

H

H

F

L

I

L

Q

-

E

E

S

S

T
x
I

K

G

G

N

E
x
K

A

-

E

-

G

-

R

-

G

-

G

-

M

L

K

R

S

E

K

A

L

L

N

K

V

I

A

A

K

K

E

H

A

S

A

E

R

-

K

-

N

-

I

-

P

-

T

V

Y

Y

I

F

A

I

N

N

G

G

N

K

V

V

D

K

N

E

M

N

I

L

L

G

D

K

I

A

V

I

D

R

G

G

K

E

E

K

V

V

G

G

T

T

K

R

V

L

binding Dimethylallyl monophosphate: G135 (≠ E145), I147 (≠ F156), S149 (≠ D158), G150 (≠ N159)
binding adenosine-5'-diphosphate: I175 (≠ L184), Y176 (= Y185), R178 (≠ G187), P180 (= P189), I203 (≠ T214), K206 (≠ E217)

Sites not aligning to the query:

binding Dimethylallyl monophosphate: 44, 49

3wwmA Crystal structure of lysz from thermus thermophilus with adp (see paper)
28% identity, 47% coverage: 134:259/268 of query aligns to 146:264/269 of 3wwmA

query
sites
3wwmA

L

L

L

L

S

Q

E

A

G

G

I

Y

I

L

P

P

I

V

A

L

N

T

E

P

D

P

D

A

A

L

V

S

S

Y

L

E

S

N

M

E

S

A

T

I

F

N

T

T

D

D

N

G

D
|
D

E

Q

L

I

A

A

S

A

L

L

V

L

A

A

E

T

L

L

T

Y

K

G

A

A

D

E

M

A

L

L

I

V

L

Y

L

L

T

S

D

N

T

V

D

P

G

G

L

L

Y

L

N

A

G

R

H
x
Y

P

P

D

D

D

E

E

A

N

S

T

L

E

V

R

R

I

E

A

I

H

P

V

V

G

E

T

R

D

I

E

E

K

D

V

P

E

E

H

Y

F

L

I

-

Q

-

E

-

S

-

T

-

K

-

G

-

E

-

A

A

E

L

G

A

R

Q

G

G

G

R

M
|
M

K

K

S

R

K
|
K

L

V

N

M

V

G

A

A

K

V

E

E

A

A

A

V

R

R

K

G

N

G

I

V

P

K

T

R

Y

V

I

V

-

F

A

A

N

D

G

A

N

R

V

V

D

E

N

N

M

P

I

I

L

R

D

R

I

A

V

L

D

S

G

G

K

E

active site: D172 (= D160), K231 (= K227)
binding adenosine-5'-diphosphate: Y200 (≠ H188), M228 (= M224)

Sites not aligning to the query:

active site: 5, 8, 38
binding adenosine-5'-diphosphate: 8, 36, 37, 38, 78

O50147 [LysW]-aminoadipate kinase; EC 2.7.2.17 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
28% identity, 47% coverage: 134:259/268 of query aligns to 146:264/269 of O50147

query
sites
O50147

L

L

L

L

S

Q

E

A

G

G

I

Y

I

L

P

P

I

V

A

L

N

T

E

P

D

P

D

A

A

L

V

S

S

Y

L

E

S

N

M

E

S

A

T

I

F

N

T

T

D

D

N

G

D

D

E

Q

L

I

A

A

S

A

L

L

V

L

A

A

E

T

L

L

T

Y

K

G

A

A

D

E

M

A

L

L

I

V

L

Y

L

L

T

S

D

N

T

V

D

P

G

G

L

L

Y

L

N

A

G

R

H

Y

P

P

D

D

D

E

E

A

N

S

T

L

E

V

R

R

I

E

A

I

H

P

V

V

G

E

T

R

D

I

E

E

K

D

V

P

E

E

H

Y

F

L

I

-

Q

-

E

-

S

-

T

-

K

-

G

-

E

-

A

A

E

L

G

A

R

Q

G

G

G
x
R

M

M

K

K

S
x
R

K
|
K

L

V

N

M

V

G

A

A

K

V

E

E

A

A

A

V

R

R

K

G

N

G

I

V

P

K

T

R

Y

V

I

V

-

F

A

A

N

D

G

A

N

R

V

V

D

E

N

N

M

P

I

I

L

R

D

R

I

A

V

L

D

S

G

G

K

E

R227 (≠ G223) mutation to E: Still binds LysW, but shows reduced activity.
R230 (≠ S226) mutation to E: Still binds LysW, but shows reduced activity.
K231 (= K227) mutation to E: Still binds LysW, but shows reduced activity.

Sites not aligning to the query:

57 H→A: Shows reduced activity.
111 R→E: Shows reduced activity.
112 R→E: Shows reduced activity.
113 K→E: Loss of activity.
117 K→E: Does not bind LysW.
123 K→E: Does not bind LysW.
125 K→E: Does not bind LysW.
128 R→E: Does not bind LysW.

7lntB Ternary complex of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus bound to benzyl monophosphate and atp (see paper)
23% identity, 40% coverage: 160:266/268 of query aligns to 152:256/260 of 7lntB

query
sites
7lntB

D

D

E

Q

L

C

A

M

S

E

L

V

V

L

A

C

E

R

L

M

T

F

K

D

A

P

D

E

M

K

L

V

I

V

L

F

L

V

T

S

D
|
D

T
x
I

D

D

G

G

L

L

Y
|
Y

N

T

G

A

H
x
D

P

P

D

K

D

T

E

D

N

K

T

K

E

A

R

R

I

L

A

I

H

G

V

E

G

V

T

T

D

R

E

K

K

K

V

L

E

D

H

E

F

A

I

L

Q

T

E

D

S

I

T

T

K

V

G

-

E

-

A

A

E

D

G

V

R

T

G

G

G

G

M

V

K

H

S

S

K

K

L

M

N

E

V

A

A

M

K

L

E

R

A

M

A

T

R

D

K

R

N

N

I

R

P

R

T

C

Y

Y

I

L

A

V

N

N

G

G

N

N

V

A

D

P

N

N

M

R

I

L

L

Y

D

S

I

L

V

L

D

K

G

G

K

E

E

T

V

V

G

T

T

C

K

T

V

V

S

A

binding adenosine-5'-diphosphate: D172 (= D180), I173 (≠ T181), Y177 (= Y185), D180 (≠ H188)

Sites not aligning to the query:

7lnuB Ternary complex of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus bound to isopentenyl monophosphate and atp (see paper)
23% identity, 40% coverage: 160:266/268 of query aligns to 153:257/261 of 7lnuB

query
sites
7lnuB

D

D

E

Q

L

C

A

M

S

E

L

V

V

L

A

C

E

R

L

M

T

F

K

D

A

P

D

E

M

K

L

V

I

V

L

F

L

V

T
x
S

D
|
D

T
x
I

D

D

G

G

L

L

Y
|
Y

N

T

G
x
A

H

D

P

P

D
x
K

D

T

E

D

N

K

T

K

E

A

R

R

I

L

A

I

H

G

V

E

G

V

T

T

D

R

E

K

K

K

V

L

E

D

H

E

F

A

I

L

Q

T

E

D

S

I

T

T

K

V

G

-

E

-

A

A

E

D

G

V

R

T

G

G

G

G

M

V

K

H

S

S

K
|
K

L

M

N

E

V

A

A

M

K

L

E

R

A

M

A

T

R

D

K

R

N

N

I

R

P

R

T

C

Y

Y

I

L

A

V

N

N

G

G

N

N

V

A

D

P

N

N

M

R

I

L

L

Y

D

S

I

L

V

L

D

K

G

G

K

E

E

T

V

V

G

T

T

C

K

T

V

V

S

A

binding adenosine-5'-diphosphate: S172 (≠ T179), D173 (= D180), I174 (≠ T181), Y178 (= Y185), A180 (≠ G187), K183 (≠ D190), K218 (= K227)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 8, 12
binding Isopentenyl phosphate: 48, 52, 53, 149, 151, 152

Query Sequence

>Echvi_2634 FitnessBrowser__Cola:Echvi_2634
MEKNTKMDNNEGPKRIVIKVGTNVMTNRDNRIVNTVLRKMVDQIAILYERGIMSVLVSSG
SVIAGKEVLGSKVGIKDKIIRRQVFSAVGQPRMMRHYYNIFQDYGMRCAQVLATKRDFDP
GKHRENMINCYEGLLSEGIIPIANEDDAVSLSMSTFTDNDELASLVAELTKADMLILLTD
TDGLYNGHPDDENTERIAHVGTDEKVEHFIQESTKGEAEGRGGMKSKLNVAKEAARKNIP
TYIANGNVDNMILDIVDGKEVGTKVSDD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory